CN103229780A - Cyantraniliprole-imidacloprid pesticide composition - Google Patents
Cyantraniliprole-imidacloprid pesticide composition Download PDFInfo
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- CN103229780A CN103229780A CN2013101411389A CN201310141138A CN103229780A CN 103229780 A CN103229780 A CN 103229780A CN 2013101411389 A CN2013101411389 A CN 2013101411389A CN 201310141138 A CN201310141138 A CN 201310141138A CN 103229780 A CN103229780 A CN 103229780A
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- imidacloprid
- bemisia tabaci
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- insect amide
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Abstract
The invention discloses a pesticide prepared from effective ingredients of cyantraniliprole and imidacloprid according to an effective mass ratio of (9:1)-(1:1.5) and application thereof, especially application thereof in controlling tobacco whiteflies. The mixing of the cyantraniliprole and the imidacloprid according to the mass ratio shows remarkable synergistic or additive action, improves the control effect on pests (especially tobacco whiteflies), reduces the consumption of field pesticides and postpones the pesticide resistance of pests.
Description
Technical field
The present invention relates to a kind of insecticide, belong to the pesticide field of crop pest control.
Background technology
Bemisia tabaci Bemisia tabaci belongs to Homoptera (Semiptera), Aleyrodidae, mainly cause harm cucumber, tomato, eggplant, cowpea, dish with multiple horticultural crops such as soybean, cabbage mustard and cauliflowers, it not only stings and inhales the host plant of causing harm, but also propagate multiple virus disease, due to the tomato yellow leaf curl virus disease (TYLCD) that causes some areas tomato canopy total crop failure that highlighted in the production is in the last few years namely propagated by Bemisia tabaci.The control of China's Bemisia tabaci is in the chemical control mode at present.Bemisia tabaci adults has certain ability of circling in the air, and is one of insect that is difficult to prevent and treat most on producing, and along with the continuous use of single insecticide, Bemisia tabaci has produced pesticide resistance in various degree to increasing insecticide.Especially in recent years rapid variation has taken place in China's Bemisia tabaci biotype, most of area was Q type Bemisia tabaci by former Type B Bemisia tabaci succession of causing harm, and host's adaptability of Q type Bemisia tabaci is more extensive, the ability of propagation geminivirus infection is higher, stronger to the patience of insecticide, therefore, Q type Bemisia tabaci replacement Type B Bemisia tabaci makes China face more challenges to the control of this worm.
A lot of researchs are verified, for most of insecticides, Q type Bemisia tabaci has stronger drug resistance and pesticide resistance than Type B Bemisia tabaci, indoor Q type resistance Bemisia tabaci population still keeps higher resistance level (Nauen et al. Pest Manag. Sci. after raising 2 years, 2002,58:868-875; Horowitz et al., Arch. Insect Biochem. Physiol., 2005,58:216-225).Domestic a lot of area all monitors the drug-fast generation of Q type Bemisia tabaci, and the resistant multiple is higher than the Type B Bemisia tabaci, and is especially obvious for nicotinic insecticide.Imidacloprid is first generation nicotinic insecticide, has characteristics such as wide spectrum, efficient, low toxicity, low-residual, and have tag, multiple activity such as stomach toxicity and interior suction, be mainly used in preventing and treating the sucking pest in field, as aleyrodid, aphid, plant hopper etc.Its insecticidal mechanism mainly is that selectivity suppresses insect nervous system nicotinic acetylcholine esterase acceptor, thereby the normal conduction of blocking-up insect central nervous system causes insect paralysis and then dead to occur.China develops Imidacloprid in phase early 1990s, and the production of Imidacloprid presents fast rise trend always since 1996, is widely used.Owing to use for a long time and frequently, insect rises obviously to its pesticide resistance.Especially in recent years the substituting of Bemisia tabaci biotype (the Q type substitutes Type B), research show that tentatively substituting of this biotype may have closely related property with the long-term and use widely of nicotinic insecticide such as Imidacloprid.The phenomenon that Imidacloprid significantly descends for the control efficiency of Q type Bemisia tabaci is also found in the field, and some areas even proposed to suspend the warning that Imidacloprid is prevented and treated Bemisia tabaci prevent and treat the field of this class pest and Imidacloprid and beaten alarm bell service life to Imidacloprid.
The bromine cyanogen insect amide is a kind of novel anthranilamide-based insecticide, belongs to second generation ryania acceptor insecticide, and its mechanism of action is by activating the ryania acceptor of target pest, discharge the calcium in striated muscle and the smooth muscle cell, causing the insect paralysis dead.High medicament insecticidal spectrum is extensive, and evidence is not only effective to lepidoptera pest, and sucking pests such as Bemisia tabaci, aphid, thrips are also had the better prevention effect.This medicament has extremely strong two-way conduction and interior absorption energy, also has certain contact toxicity, no cross resistance (Li et al. Pest Manag. Sci. such as evidence bromine cyanogen insect amide and anabasine and pyrrole propyl ether, 2012,68:83-81), therefore having the Bemisia tabaci field population that nicotinic insecticide is developed immunity to drugs will have higher cytotoxicity and the good anti-effect of gram.
In order to overcome Bemisia tabaci to the higher pesticide resistance of Imidacloprid and other nicotinic insecticides, especially the pesticide resistance of Q type Bemisia tabaci, simultaneously in order to prolong the service life of cheap Imidacloprid, improve the control efficiency of Bemisia tabaci, reduce the usage amount of field insecticide, be necessary to seek or research and develop new insecticide.
Summary of the invention
At the deficiencies in the prior art and demand, the present invention has filtered out the high co-toxicity of ratio range these two mechanism of action of bromine cyanogen insect amide and Imidacloprid different insecticides has to(for) Bemisia tabaci.
Technical problem to be solved by this invention is: a kind of low toxicity, low-residual are provided, are suitable for the efficient chemical control insecticide of vegetables field Bemisia tabaci.
Technical scheme provided by the invention is: a kind of insecticide, its active ingredient are to be made of bromine cyanogen insect amide and the Imidacloprid mass ratio 9:1-1:1.5 according to active ingredient.
Preferably, the mass ratio of described bromine cyanogen insect amide and Imidacloprid active ingredient is 9:1-7:3.
More preferably, the mass ratio of described bromine cyanogen insect amide and Imidacloprid active ingredient is 9:1 or 4:1.
Insecticide of the present invention can be mixed with agricultural and go up wettability pulvis, suspending agent, granule, water dispersible granules, missible oil and the microemulsion formulation etc. that allow.
It is sucking pest that described insecticide is applied to prevent and treat crop pest, in particular for preventing and treating Bemisia tabaci.
The present invention has following beneficial effect: the present invention filters out the composition pesticide with synergistic effect that contains bromine cyanogen insect amide and Imidacloprid, when bromine cyanogen insect amide and Imidacloprid carry out proportioning according to effective mass than 9:1-1:1.5, show tangible synergy or summation action when the Bemisia tabaci of control vegetables field, the synergistic effect during wherein with the 9:1-7:3 ratio is more obvious.Insecticides low toxicity of the present invention, low-residual, be suitable for the efficient chemical control of vegetables field Bemisia tabaci, simultaneously have the double effect of controlling for other suckings pest such as aphid, thrips, this composition can reduce field insecticide consumption, slow down the service life of the development of sucking pest resistance to insecticide and prolongation nicotinic insecticide etc.
Embodiment
Detailed description below by embodiment is further illustrated the present invention, but is not limitation of the present invention, only does the example explanation.
For examination worm source and medicament
The Bemisia tabaci that adopts in the test Bemisia tabaci population that to be this seminar gathered on the eggplant of Haidian District Beijing field " assorted No. 16 of circle " in 2012 indoorly is accredited as the Q type through biotype.Surpassed for 3 generations in indoor feeding before the proportioning screening test, do not contact any insecticide.
But supplying the examination insecticide is 10% bromine cyanogen insect amide dispersed oil suspending agent (du pont company product); 10% imidacloprid wettable powder (Hebei Weiyuan Biochemical Co., Ltd.'s product).
Host's blade that the test of indoor Bemisia tabaci adults toxicity test is used by the no worm wild cabbage of cultivating (
Brassica oleraceaL. var.
Capitata) obtain on the seedling, kind is " rich No. 1 of capital ".
Data are handled and computational methods
Test data adopts each concentration of Probit software analysis to handle the dead ratio of different time points Bemisia tabaci, draws slope, the LC of each medicament and different proportionings
50And 95% confidence limit etc.The lethality of blank can not surpass 10%, otherwise writes off, and repeats this test.The computational methods of the different proportioning co-toxicity coefficients with Imidacloprid of bromine cyanogen insect amide (CTC) are carried out according to the abundant method of Sun Yun, actual and the theoretical toxicity index (ATI, TTI) of single agent toxicity index (TI) of each proportioning of difference, mixture (being made as M), last co-toxicity coefficient (CTC) of being tried to achieve each proportioning by ATI/TTI.Judge according to being total to poison dilution CTC whether the two exists synergistic effect.Be antagonism when CTC<80,80~120 is summation action, and>120 o'clock is synergistic effect, and has only the ratio that is mixed when CTC>200 usually, just is regarded as having remarkable synergistic effect in actual production.
The The data DPS v3.01 professional version that efficiency test investigation obtains is calculated bromine cyanogen insect amide and the agent of Imidacloprid list and different proportioning thereof to the relative control effect of Bemisia tabaci adults, handles the differences significance and adopts the LSD method to analyze.Computational methods are two formula of face as follows.
Insect population rate (%)=[insect population radix before (medicine before insect population radix-medicine after insect population number)/medicine] * 100 of going down
Control efficiency (%)=[(insect population of processing go down the insect population of rate-blank go down rate)/(insect population of 100-blank go down rate)] * 100
Embodiment one: the Bemisia tabaci adults Toxicity Determination
On the basis of preliminary experiment, bromine cyanogen insect amide and Imidacloprid are measured it to the co-toxicity of Bemisia tabaci adults according to the scope that the mass ratio of active ingredient is set to 9:1~1:9 respectively.On the basis of calculating two kinds of insecticide consumptions, take by weighing an amount of bromine cyanogen insect amide and Imidacloprid respectively, add suitable quantity of water again it is dissolved to desired concn, namely be used for following agar leaf dipping method determination test.
The toxicity test of Bemisia tabaci adults is carried out in the glass dactylethrae.Specific operation process is as follows: spread 1.8%~2% agar 2mL in glass dactylethrae bottom, treat that agar solidifies the back and wipes away main wall steam with blotting paper.Gathering wild cabbage not dispenser, smooth does not have the blade of worm seedling, gets the leaf disk that diameter is 22mm with card punch, avoids thick vein place as far as possible.Test arranges 5~7 concentration, and establishes the clear water blank, and every processing arranges 4 repetitions.After preparing soup, each roundleaf sheet soaked in the soup of variable concentrations 10 seconds, and it is smooth in the pipe end that room temperature is dried the back face down.The dactylethrae mouth of pipe is patted and is stopped the blade have for the examination Bemisia tabaci Bemisia tabaci adults is freely flown into down, and 20~35 of every pipes are filled in 2/3 pipe range with tampon, keep with blade pitch from being not less than 15mm.Then dactylethrae is inverted and is put in illumination box, 25 ± 1 ℃ of temperature are set, the photoperiod is 14h: 8h(L: D), check death toll and the survival number of Bemisia tabaci in each pipe behind the 48h.
Bromine cyanogen insect amide and Imidacloprid and the toxicity test concrete outcome that the two mixes Bemisia tabaci according to different proportionings see Table 1.
Table 1 bromine cyanogen insect amide and Imidacloprid are to the co-toxicity of Bemisia tabaci
Table 1 has shown under bromine cyanogen insect amide and the agent of Imidacloprid list and the different proportioning situation thereof, to the indoor virulence of Bemisia tabaci adults.The bromine cyanogen insect amide is to the LC of Bemisia tabaci
50Be 54.41mg/L, Imidacloprid is to the LC of Bemisia tabaci
50Be 238.57mg/L.By the abundant method result of calculation of Sun Yun as can be known, increasing gradually of minimizing, Imidacloprid adding proportion gradually along with bromine cyanogen insect amide ratio in the compound, compound reduces gradually to the cytotoxicity of Bemisia tabaci adults, be that the LC50 value raises gradually, but its co-toxicity coefficient changes to some extent, when mixing with the 9:1-7:3 ratio with bromine cyanogen insect amide and Imidacloprid, the CTC value is greater than 120, illustrate that the aforementioned proportion scope is synergistic effect, when wherein mixing with the 9:1 ratio with bromine cyanogen insect amide and Imidacloprid, synergistic effect is the most remarkable, and co-toxicity coefficient CTC is 492.58.And 1.5:1-1:1.5 presents summation action, and bad reaction is not seen in indoor mixing, so field efficiency test is carried out in next step simulation.
Embodiment two: the efficiency test of each medicament and different proportionings thereof
Field efficiency test carries out at the no worm cotton seedling " middle cotton 49 " of indoor pot, and cotton is 4-6 sheet true leaf during test.The mass ratio that bromine cyanogen insect amide (No. 1), Imidacloprid (No. 2), bromine cyanogen insect amide and Imidacloprid active ingredient are set respectively is respectively 9:1(3 number), 4:1(4 number), 7:3(5 number), 1.5:1(6 number), 1:1(7 number), 1:1.5(8 number), 3:7(9 number), 1:4(10 number) with 1:9(11 number) etc. 11 different chemicals treatment, add clear water contrast, totally 12 different disposal.Every strain cotton seedling is as 1 repetition, and each processing arranges 4 strain cotton seedlings, repeats as 4 times respectively.But measure 10% an amount of bromine cyanogen insect amide dispersed oil suspending agent and 10% Imidacloprid preparation wetting powder respectively, after then the two mass ratio according to the active ingredient of 9:1,4:1,7:3,1.5:1,1:1,1:1.5,3:7,1:4 and 1:9 thoroughly being mixed, get the 1-11 Treatment Solution respectively, dilute 2000 times of liquid, it is standby to spray.The liquid medicine of different disposal is all now with the current.
Put into the small-sized gauze tent that is placed with the cotton seedling respectively in getting Bemisia tabaci adults at dusk, treat Bemisia tabaci adults radix in the every strain of its stable " Invest, Then Investigate " the next morning.Carry out the positive and negative blade face of complete stool even spraying with manual sprayer then, (No. 12) are handled in the clear water spraying in contrast.Afterwards respectively at 1 day, 3 days and each Bemisia tabaci adults quantity of handling of investigation in 7 days behind the medicine.
Investigate the insect population radix of variant processing before the spraying efficiency test, investigated the quantity of respectively handling Bemisia tabaci adults respectively in 1 day, 3 days and 7 days after the dispenser, different chemicals treatment are to relative control effect and the significance of difference analysis in table 2 of Bemisia tabaci in different control times.
Table 2 bromine cyanogen insect amide and Imidacloprid and different proportioning thereof are to the control efficiency of Bemisia tabaci adults relatively
Annotate: data are the mean value of 4 repetitions in the table.Lowercase is identical, and to be illustrated in the p=0.05 level difference not remarkable, and showing at the p=0.01 level difference that capitalization is identical is not remarkable.
As can be seen from Table 2, along with the prolongation of spraying time, the relative control effect of each chemicals treatment all rises to some extent, but not obvious.Behind the medicine 1 day, bromine cyanogen insect amide and Imidacloprid all show certain quick-acting, its relative control effect is respectively 81.47% and 50.07%, wherein Imidacloprid list agent preventive effect significantly is lower than the relative control effect of all the other each processing, the bromine cyanogen insect amide is then a little less than the relative control effect under the 1:1.5 proportioning, and difference is not remarkable.Bromine cyanogen insect amide and Imidacloprid show obvious synergistic effect during according to the mass ratio of 9:1,4:1 and 7:3 active ingredient, the control efficiency of the additive combination ratio of relative control effect in the 1.5:1-1:1.5 scope; Along with the further minimizing (when itself and Imidacloprid ratio are 3:7,1:4 and 1:9) of bromine cyanogen insect amide effective mass ratio, the relative control effect of its combination descends, and significantly is lower than the preventive effect of all the other ratios, but still is higher than the control efficiency of Imidacloprid list agent.3 days trend during with 7 days is consistent behind the medicine.
Therefore, the field analog reslt further shows, when bromine cyanogen insect amide and Imidacloprid carry out proportioning according to the quality 9:1-1:1.5 of active ingredient, control efficiency when the relative control effect of 1 day, 3 days and 7 days all is higher than two kinds of single agent and deals with separately behind the medicine, the synergistic effect during wherein with the 9:1-7:3 ratio is more obvious.
Claims (6)
1. insecticide is characterized in that: its active ingredient is to be made of bromine cyanogen insect amide and the Imidacloprid mass ratio 9:1-1:1.5 according to active ingredient.
2. insecticide according to claim 1, it is characterized in that: the mass ratio of described bromine cyanogen insect amide and Imidacloprid active ingredient is 9:1-7:3.
3. insecticide according to claim 2, it is characterized in that: the mass ratio of described bromine cyanogen insect amide and Imidacloprid active ingredient is 9:1 or 4:1.
4. according to each described insecticide of claim 1-3, it is characterized in that: described insecticide is wettability pulvis, suspending agent, granule, water dispersible granules, missible oil or microemulsion formulation.
5. according to the application of each described insecticide of claim 1 to 4 in control sucking mouth parts crop pest.
6. application according to claim 5 is characterized in that: it is used for preventing and treating Bemisia tabaci.
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CN104054741A (en) * | 2014-06-24 | 2014-09-24 | 北京燕化永乐生物科技股份有限公司 | Insecticidal composition |
CN105284876A (en) * | 2015-12-02 | 2016-02-03 | 河北省农林科学院植物保护研究所 | Insecticide composition for preventing and treating cylas formicarius |
CN106070295A (en) * | 2016-06-21 | 2016-11-09 | 南京华洲药业有限公司 | A kind of brominated cyanogen insect amide and the Synergistic insecticidal compositions of imidacloprid and application thereof |
CN109100443A (en) * | 2018-09-30 | 2018-12-28 | 浙江省检验检疫科学技术研究院 | The method of various new nicotinoids drug and its metabolite residue amount in royal jelly is measured simultaneously |
CN110495469A (en) * | 2019-10-09 | 2019-11-26 | 江苏钟山化工有限公司 | A kind of composition pesticide and its application |
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Publication number | Priority date | Publication date | Assignee | Title |
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CN104054741A (en) * | 2014-06-24 | 2014-09-24 | 北京燕化永乐生物科技股份有限公司 | Insecticidal composition |
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CN110495469A (en) * | 2019-10-09 | 2019-11-26 | 江苏钟山化工有限公司 | A kind of composition pesticide and its application |
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