CN103224546B - Hydrated carbamyl glycine dipeptide chelated iron and preparation method thereof - Google Patents
Hydrated carbamyl glycine dipeptide chelated iron and preparation method thereof Download PDFInfo
- Publication number
- CN103224546B CN103224546B CN201310142525.4A CN201310142525A CN103224546B CN 103224546 B CN103224546 B CN 103224546B CN 201310142525 A CN201310142525 A CN 201310142525A CN 103224546 B CN103224546 B CN 103224546B
- Authority
- CN
- China
- Prior art keywords
- carbamoylglycine
- iron
- dipeptide
- carbamyl
- dipeptides
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 title claims abstract description 117
- 229910052742 iron Inorganic materials 0.000 title claims abstract description 59
- 238000002360 preparation method Methods 0.000 title claims abstract description 13
- -1 carbamyl glycine dipeptide Chemical compound 0.000 title abstract description 7
- 229910000359 iron(II) sulfate Inorganic materials 0.000 claims abstract description 15
- 239000011790 ferrous sulphate Substances 0.000 claims abstract description 10
- 235000003891 ferrous sulphate Nutrition 0.000 claims abstract description 10
- BAUYGSIQEAFULO-UHFFFAOYSA-L iron(2+) sulfate (anhydrous) Chemical compound [Fe+2].[O-]S([O-])(=O)=O BAUYGSIQEAFULO-UHFFFAOYSA-L 0.000 claims abstract 5
- 108010016626 Dipeptides Proteins 0.000 claims description 54
- KZVRXPPUJQRGFN-UHFFFAOYSA-N N-carbamoylglycine Chemical compound NC(=O)NCC(O)=O KZVRXPPUJQRGFN-UHFFFAOYSA-N 0.000 claims description 49
- 239000013522 chelant Substances 0.000 claims description 30
- 230000036571 hydration Effects 0.000 claims description 24
- 238000006703 hydration reaction Methods 0.000 claims description 24
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 18
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 claims description 8
- 238000001816 cooling Methods 0.000 claims description 6
- 238000002425 crystallisation Methods 0.000 claims description 6
- 238000001035 drying Methods 0.000 claims description 2
- 238000001914 filtration Methods 0.000 claims description 2
- 238000004519 manufacturing process Methods 0.000 abstract description 9
- 238000000034 method Methods 0.000 abstract description 6
- 239000000047 product Substances 0.000 abstract description 6
- 239000003960 organic solvent Substances 0.000 abstract description 5
- 239000002994 raw material Substances 0.000 abstract description 5
- 238000005516 engineering process Methods 0.000 abstract description 4
- 210000002966 serum Anatomy 0.000 abstract description 4
- 239000011573 trace mineral Substances 0.000 abstract description 4
- 235000013619 trace mineral Nutrition 0.000 abstract description 4
- 229910052751 metal Inorganic materials 0.000 abstract description 3
- 239000007864 aqueous solution Substances 0.000 abstract description 2
- 230000008569 process Effects 0.000 abstract description 2
- 241000282887 Suidae Species 0.000 abstract 1
- 235000019730 animal feed additive Nutrition 0.000 abstract 1
- 238000009776 industrial production Methods 0.000 abstract 1
- 230000003647 oxidation Effects 0.000 abstract 1
- 238000007254 oxidation reaction Methods 0.000 abstract 1
- 239000002244 precipitate Substances 0.000 abstract 1
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 12
- SURQXAFEQWPFPV-UHFFFAOYSA-L iron(2+) sulfate heptahydrate Chemical compound O.O.O.O.O.O.O.[Fe+2].[O-]S([O-])(=O)=O SURQXAFEQWPFPV-UHFFFAOYSA-L 0.000 description 10
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 8
- 239000002689 soil Substances 0.000 description 7
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 6
- 238000012360 testing method Methods 0.000 description 6
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 5
- 229910052757 nitrogen Inorganic materials 0.000 description 5
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 description 4
- 206010022971 Iron Deficiencies Diseases 0.000 description 4
- 238000010521 absorption reaction Methods 0.000 description 4
- 208000007502 anemia Diseases 0.000 description 4
- 230000008025 crystallization Effects 0.000 description 4
- 239000013642 negative control Substances 0.000 description 4
- 239000013641 positive control Substances 0.000 description 4
- 239000007787 solid Substances 0.000 description 4
- 238000003756 stirring Methods 0.000 description 4
- 238000000967 suction filtration Methods 0.000 description 4
- 238000001291 vacuum drying Methods 0.000 description 4
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 3
- 230000003078 antioxidant effect Effects 0.000 description 3
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 3
- 235000014590 basal diet Nutrition 0.000 description 3
- 125000003178 carboxy group Chemical group [H]OC(*)=O 0.000 description 3
- 239000013078 crystal Substances 0.000 description 3
- 230000002354 daily effect Effects 0.000 description 3
- 238000002329 infrared spectrum Methods 0.000 description 3
- 229910052760 oxygen Inorganic materials 0.000 description 3
- 239000001301 oxygen Substances 0.000 description 3
- 239000002245 particle Substances 0.000 description 3
- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 description 3
- 230000032258 transport Effects 0.000 description 3
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 description 2
- HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 description 2
- 238000002835 absorbance Methods 0.000 description 2
- 238000004458 analytical method Methods 0.000 description 2
- 239000007795 chemical reaction product Substances 0.000 description 2
- 150000001875 compounds Chemical class 0.000 description 2
- 229910052802 copper Inorganic materials 0.000 description 2
- 239000010949 copper Substances 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 235000012631 food intake Nutrition 0.000 description 2
- 238000005259 measurement Methods 0.000 description 2
- 230000035790 physiological processes and functions Effects 0.000 description 2
- 238000003860 storage Methods 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 230000003442 weekly effect Effects 0.000 description 2
- 239000011701 zinc Substances 0.000 description 2
- 229910052725 zinc Inorganic materials 0.000 description 2
- 206010006895 Cachexia Diseases 0.000 description 1
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 1
- 235000019750 Crude protein Nutrition 0.000 description 1
- 206010012735 Diarrhoea Diseases 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- PWHULOQIROXLJO-UHFFFAOYSA-N Manganese Chemical compound [Mn] PWHULOQIROXLJO-UHFFFAOYSA-N 0.000 description 1
- 244000046052 Phaseolus vulgaris Species 0.000 description 1
- 235000010627 Phaseolus vulgaris Nutrition 0.000 description 1
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 1
- 206010048222 Xerosis Diseases 0.000 description 1
- 240000008042 Zea mays Species 0.000 description 1
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 1
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 230000000996 additive effect Effects 0.000 description 1
- 239000003674 animal food additive Substances 0.000 description 1
- 239000003963 antioxidant agent Substances 0.000 description 1
- 230000036528 appetite Effects 0.000 description 1
- 235000019789 appetite Nutrition 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 230000037396 body weight Effects 0.000 description 1
- 239000011575 calcium Substances 0.000 description 1
- 229910052791 calcium Inorganic materials 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- 239000001569 carbon dioxide Substances 0.000 description 1
- 229910002092 carbon dioxide Inorganic materials 0.000 description 1
- 235000011089 carbon dioxide Nutrition 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 235000005822 corn Nutrition 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000003745 diagnosis Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 230000037149 energy metabolism Effects 0.000 description 1
- 238000003912 environmental pollution Methods 0.000 description 1
- DNJIEGIFACGWOD-UHFFFAOYSA-N ethanethiol Chemical compound CCS DNJIEGIFACGWOD-UHFFFAOYSA-N 0.000 description 1
- 230000003203 everyday effect Effects 0.000 description 1
- 235000021050 feed intake Nutrition 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 229910052739 hydrogen Inorganic materials 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-M hydroxide Chemical compound [OH-] XLYOFNOQVPJJNP-UHFFFAOYSA-M 0.000 description 1
- ZHUXMBYIONRQQX-UHFFFAOYSA-N hydroxidodioxidocarbon(.) Chemical compound [O]C(O)=O ZHUXMBYIONRQQX-UHFFFAOYSA-N 0.000 description 1
- 125000002887 hydroxy group Chemical group [H]O* 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 238000001802 infusion Methods 0.000 description 1
- 229910052500 inorganic mineral Inorganic materials 0.000 description 1
- 210000000936 intestine Anatomy 0.000 description 1
- 230000010438 iron metabolism Effects 0.000 description 1
- 210000004185 liver Anatomy 0.000 description 1
- 229910052748 manganese Inorganic materials 0.000 description 1
- 239000011572 manganese Substances 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 239000011707 mineral Substances 0.000 description 1
- 235000010755 mineral Nutrition 0.000 description 1
- 210000003205 muscle Anatomy 0.000 description 1
- 238000011056 performance test Methods 0.000 description 1
- 229910052698 phosphorus Inorganic materials 0.000 description 1
- 239000011574 phosphorus Substances 0.000 description 1
- 238000001243 protein synthesis Methods 0.000 description 1
- 235000019633 pungent taste Nutrition 0.000 description 1
- 238000005527 soil sampling Methods 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 210000000952 spleen Anatomy 0.000 description 1
- 210000002784 stomach Anatomy 0.000 description 1
- 239000013589 supplement Substances 0.000 description 1
- 230000014616 translation Effects 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
- 208000016318 wasting Diseases 0.000 description 1
- 230000004584 weight gain Effects 0.000 description 1
- 235000019786 weight gain Nutrition 0.000 description 1
Landscapes
- Fodder In General (AREA)
- Peptides Or Proteins (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Abstract
The invention discloses hydrated carbamyl glycine dipeptide chelated iron and a preparation method thereof. The invention uses carbamyl glycine dipeptide and ferrous sulfate as main raw materials, and directly precipitates crystalline hydrated carbamyl glycine dipeptide chelated iron in aqueous solution without using organic solvent. The method has the advantages of easily available raw materials, simple process, low cost, high product purity, stable quality, convenient technology for industrial production and the like. The hydrated carbamyl glycine dipeptide chelated iron can be used as an animal feed additive, the production performance of weaned pigs is improved, the content of trace elements in serum is improved, the content of metal elements in excrement is reduced, and the oxidation resistance of organisms is improved.
Description
Technical field
The invention belongs to field of feed additive technology, particularly hydration carbamoylglycine dipeptide iron chelate and preparation method thereof.
Background technology
Iron is one of the most necessary trace element of animal body.In animal body, the iron of nearly 2/3rds is present in the myohaemoglobin of erythrocytic oxyphorase and muscle, and the iron of 20% is present in liver, spleen and its hetero-organization in different forms.The main Physiological Function of iron transports oxygen and carbon dioxide by oxyphorase, and play a part fixing by myohaemoglobin and store oxygen.Modern study proves, iron and energy metabolism closely related because its biochemical action of competence exertion when having enzyme over half and factor iron content in tricarboxylic cycle or only have iron to exist, complete physiological function.Iron also affects protein synthesis in animal body and immunity function.
Utilizing of iron deficiency or iron is bad, and will cause the disturbed metabolic processes such as the transport of oxygen, storage, the transport of carbonic acid gas and redox, impact is grown and even the various diseases such as anaemia occurred.Piglet stores iron or takes the photograph iron deficiency, then occur hypoferric anemia.Children sucking pig in age (especially nascent) easily suffer from iron deficiency, this is because in its body, iron storage is relatively low.Piglet iron deficiency shows as poor appetite, and poor growth is day by day become thin, and burnout happiness is sleeping, and breathe urgent easily, rough coat, xerosis cutis lacks flexibility, sometimes laxativeness, and ight soil is lead, stench.Blood has obvious change, in incarnadine, thin in water, and this is the important evidence of diagnosis piglet anaemia disease.Piglet gently then forms cad pig after suffering from anemia, heavy then death in 3 ~ 4 weeks.
Animal supplements inorganic iron usage quantity greatly, easily causes a large amount of wastings of resources.The iron ion of feed intake and hydroxide ion easily form the polymkeric substance that insoluble hydroxyl closes complex compound, thus reduce its absorption rate.Therefore only have little part (5% ~ 8%) to be absorbed, remaining is then discharged with ight soil by enteron aisle, easily causes environmental pollution.
Summary of the invention
The object of the invention is to the deficiency overcoming prior art, a kind of hydration carbamoylglycine dipeptide iron chelate and preparation method thereof is provided.
The technical solution used in the present invention is:
The preparation method of hydration carbamoylglycine dipeptide iron chelate, comprises the following steps:
1) by water-soluble for carbamoylglycine dipeptides, adjust pH to be 5.0 ~ 6.0, be heated to 50 ~ 70 DEG C;
2) add xitix and ferrous sulfate successively again, 60 ~ 80 DEG C are reacted 0.5 ~ 1 hour;
3) hydration carbamoylglycine dipeptide iron chelate is obtained after crystallisation by cooling, filtration, drying.
Preferably, the mol ratio of carbamoylglycine dipeptides and ferrous sulfate is (1.7 ~ 2.3): 1.
More excellent, carbamoylglycine dipeptides and ferrous sulfate mole be 2:1.
Preferably, the consumption of xitix be carbamoylglycine dipeptides, ferrous sulfate and water quality and 2 ‰ ~ 3 ‰.
Preferably, in step 1), the mass volume ratio of carbamoylglycine dipeptides and water is 1g:(2.0 ~ 3.0) ml.
More excellent, the mass volume ratio of carbamoylglycine dipeptides and water is 1g:2.5ml.
The invention has the beneficial effects as follows:
The present invention is direct crystallize out hydration carbamoylglycine dipeptide iron chelate in aqueous, do not need with an organic solvent, the inventive method has that raw material is easy to get, technique is simple, cost is low, product purity is high, steady quality, technology are convenient to the advantages such as suitability for industrialized production.
Hydration carbamoylglycine dipeptide iron chelate of the present invention can be used as animal feedstuff additive, improves the production performance of weanling pig, improves the content of Trace Elements In Serum, reduces the content of metallic element in ight soil, improves the antioxidant property of body.
Accompanying drawing explanation
Fig. 1 is the infrared spectrogram of hydration carbamoylglycine dipeptide iron chelate;
Fig. 2 is the infrared spectrogram of carbamoylglycine dipeptides.
Embodiment
The present invention utilizes carbamoylglycine dipeptides, ferrous sulfate as main raw material, according to the difference of end product saturation solubility at different temperatures, by reducing temperature, makes end product directly crystallize out from inorganic aqueous solution.It is a kind of inner complex containing crystal water that the present invention obtains product, and namely hydration carbamoylglycine dipeptide iron chelate, at the uniform velocity discharges in animal body, little to intestines and stomach pungency, as organic molecule, is easy to directly be absorbed by animal.Owing to not containing organic solvent in production technique of the present invention, when dropping into suitability for industrialized production, decrease the infusion of financial resources of raw material and equipment, reduce the impact on production safety and environment.
Below in conjunction with embodiment, the present invention is further illustrated, but be not limited thereto.
embodiment 1
The preparation method of hydration carbamoylglycine dipeptide iron chelate, its concrete steps are as follows:
Take 23.6g(0.2mol) carbamoylglycine dipeptides, the 60ml that adds water dissolves, and regulates pH to 5.0, be heated to 60 DEG C with sodium hydroxide; Add 0.35g xitix again, 27.8g(0.1mol) iron vitriol, control temperature, at 60 DEG C, reacts 1.0 hours; Cooling and stirring crystallization, suction filtration, vacuum-drying under gained solid 60 DEG C of conditions, obtains hydration carbamoylglycine dipeptide iron chelate 29.2g.
Detecting iron level in dipeptide iron chelate is 13.72%, and nitrogen content is 13.84%.
embodiment 2
The preparation method of hydration carbamoylglycine dipeptide iron chelate, its concrete steps are as follows:
Take 40.12g(0.34mol) carbamoylglycine dipeptides, the 80ml that adds water dissolves, and regulates pH to 6.0, be heated to 50 DEG C with sodium hydroxide; Add 0.70g xitix again, 55.6g(0.2mol) iron vitriol, control temperature, at 70 DEG C, reacts 0.5 hour; Cooling and stirring crystallization, suction filtration, the vacuum-drying at 60 DEG C of gained solid, obtains hydration carbamoylglycine dipeptide iron chelate 59.4g.
Detecting iron level in dipeptide iron chelate is 13.88%, and nitrogen content is 13.76%.
embodiment 3
The preparation method of hydration carbamoylglycine dipeptide iron chelate, its concrete steps are as follows:
Take 74.34g(0.63mol) carbamoylglycine dipeptides, the 193ml that adds water dissolves, and regulates pH to 5.0, be heated to 55 DEG C with sodium hydroxide; Add 1.05g xitix again, 83.4g(0.3mol) iron vitriol, control temperature, at 80 DEG C, reacts 1.0 hours; Cooling and stirring crystallization, suction filtration, the vacuum-drying at 60 DEG C of gained solid, obtains hydration carbamoylglycine dipeptide iron chelate 85.8g.
Detecting iron level in dipeptide iron chelate is 13.81%, and nitrogen content is 13.79%.
embodiment 4
The preparation method of hydration carbamoylglycine dipeptide iron chelate, its concrete steps are as follows:
Take 108.56g(0.92mol) carbamoylglycine dipeptides, the 326ml that adds water dissolves, and regulates pH to 5.0, be heated to 70 DEG C with sodium hydroxide; Add 1.40g xitix again, 111.2g(0.4mol) iron vitriol, control temperature, at 80 DEG C, reacts 0.5 hour; Cooling and stirring crystallization, suction filtration, the vacuum-drying at 60 DEG C of gained solid, obtains hydration carbamoylglycine dipeptide iron chelate 116.8g.
Detecting iron level in dipeptide iron chelate is 13.87%, and nitrogen content is 13.82%.
Product absolute ethanol washing embodiment 1 obtained, dry, can obtain the hydration carbamoylglycine dipeptide iron chelate that purity is higher, be pale yellow crystals particle, soluble in water, is insoluble to ethanol, methyl alcohol, acetone and other organic solvent.Sample presentation surveys infrared spectra, and as shown in Figure 1, the infrared spectrogram of corresponding carbamoylglycine dipeptides as shown in Figure 2 for gained collection of illustrative plates.
Fig. 2 is the infrared spectrogram of carbamoylglycine dipeptides, and in carbamoylglycine dipeptides, amino and carboxyl are respectively with H
3n
+and COO
-state exist, carbamoylglycine dipeptides is at 3228.14cm
-1there is wider absorption band at place, and this is amino charateristic avsorption band, and the antisymmetric stretching vibration of carboxylic acid ion in carbamoylglycine dipeptides and symmetrical stretching vibration are at 1541.81cm
-1and 1241.55cm
-1.Known by Fig. 1, at 3359.12cm
-1there is wider absorption peak in place, shows to have the existence of water molecules or the existence of OH in inner complex.Another at 1596.74cm
-1occur water in carboxyl stretching vibration absorb and at 1313.47cm
-1the absorption of the O-H at place, shows the existence having water molecules in carbamoylglycine dipeptide iron chelate.
The infrared spectra of comparison diagram 1 and Fig. 2 is known, after formation inner complex, and H
3n
+characteristic absorbance there occurs red shift, simultaneously the characteristic absorbance of carboxyl is blue shifted to 1596.74cm
-1, 1313.47cm
-1place, this is because the amino of carbamoylglycine dipeptides and carboxyl oxygen participate in the result that coordination forms inner complex.
The crystalline particle sample presentation of this dipeptide iron chelate is carried out ultimate analysis, according to measurement result, infers that the molecular formula of this chelated iron is for (C
6h
10n
4o
6fe) 6H
2o.The measurement result of ultimate analysis and calculated value contrast as shown in the table:
| ? | Fe | C | H | N |
| Theoretical value | 14.03% | 18.10% | 5.57% | 14.07% |
| Measured value | 13.96% | 18.16% | 5.57% | 13.92% |
Examples of implementation 2 ~ 4 products obtained therefrom, through same way process, all obtains pale yellow crystals particle, soluble in water, is insoluble to ethanol, methyl alcohol, acetone and other organic solvent, and sample presentation detects, and gained infrared spectra and Fig. 1 are extremely similar, are characterized by same compound.The content of Fe, C, H, N is all close to above-mentioned theory value.
animal experiment:
The hydration carbamoylglycine dipeptide iron chelate prepared with embodiment 1 below carries out animal experiment.
1, test is arranged
The 21 age in days weanling pig 90 select close batch, body weight is close, being in a good state of health, raises one week in advance.Be divided at random 5 groups (1 negative control group, 1 positive control group, 3 test group), often organize 3 repetitions, each repetition 6 piglets.Test 21 d by a definite date.Duration of test, free choice feeding is drunk water; Natural lighting, temperature is at 22-28 DEG C, and humidity is at 60-70%.The crude protein content of basal diet is 18.0%, and metabolizable energy is 3400Kcal/kg, and calcium contents is 0.8%, and available phosphorus content is 0.35%.
Wherein, the feed of negative control group is corn bean pulp type basal diet, and mineral substance does not add iron, and iron is less than 3PPm; Based on the feed of positive control group, daily ration adds the iron vitriol of iron content 12ppm; Based on the feed of 3 test group, daily ration adds hydration carbamoylglycine dipeptide iron chelate, iron-holder is respectively 6,12,18ppm.
2, production performance test
Food consumption, the ight soil index of time recording every day every hurdle piglet; Measure weekly the individual weight of each piglet; Add up feedstuff-meat ratio weekly, calculate the production performances such as all food consumptions, day weight gain; The content of ight soil sampling determination iron, copper, zinc, manganese; Measure body Antioxidant Indexes SOD vigor and GSH-Px vigor.
3, test-results
The weanling pig average daily gain of 3 test group significantly improves (P < 0.05), and diarrhea rate significantly reduces (P < 0.05), and Iron in Serum content significantly improves (P < 0.05); In 12ppm, 18ppm interpolation group piglet ight soil, iron level significantly reduces (P < 0.05), and in ight soil, copper, zinc, Fe content are all lower than positive and negative control group, and reduces along with the increase of hydration carbamoylglycine dipeptide iron chelate addition; The feedstuff-meat ratio of 12ppm interpolation group piglet is minimum, and SOD vigor and GSH-Px vigor significantly improve (P < 0.05) than positive and negative control group.
Result shows: appropriate interpolation product hydration carbamoylglycine dipeptide iron chelate of the present invention in basal diet, the production performance of weanling pig can be improved to a certain extent, improve the content of Trace Elements In Serum, reduce the content of metallic element in ight soil, improve the antioxidant property of body.
Claims (3)
1. the preparation method of hydration carbamoylglycine dipeptide iron chelate, comprises the following steps:
1) by water-soluble for carbamoylglycine dipeptides, adjust pH to be 5.0 ~ 6.0, be heated to 50 ~ 70 DEG C;
2) add xitix and ferrous sulfate successively again, 60 ~ 80 DEG C are reacted 0.5 ~ 1 hour;
3) hydration carbamoylglycine dipeptide iron chelate is obtained after crystallisation by cooling, filtration, drying;
The mol ratio of carbamoylglycine dipeptides and ferrous sulfate is (1.7 ~ 2.3): 1;
The consumption of xitix be carbamoylglycine dipeptides, ferrous sulfate and water quality and 2 ‰ ~ 3 ‰;
In step 1), the mass volume ratio of carbamoylglycine dipeptides and water is 1g:(2.0 ~ 3.0) ml.
2. preparation method according to claim 1, is characterized in that: carbamoylglycine dipeptides and ferrous sulfate mole be 2:1.
3. preparation method according to claim 1, is characterized in that: the mass volume ratio of carbamoylglycine dipeptides and water is 1g:2.5ml.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310142525.4A CN103224546B (en) | 2013-05-06 | 2013-05-06 | Hydrated carbamyl glycine dipeptide chelated iron and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310142525.4A CN103224546B (en) | 2013-05-06 | 2013-05-06 | Hydrated carbamyl glycine dipeptide chelated iron and preparation method thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103224546A CN103224546A (en) | 2013-07-31 |
| CN103224546B true CN103224546B (en) | 2015-01-28 |
Family
ID=48835206
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310142525.4A Active CN103224546B (en) | 2013-05-06 | 2013-05-06 | Hydrated carbamyl glycine dipeptide chelated iron and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103224546B (en) |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106804893A (en) * | 2016-06-14 | 2017-06-09 | 张品林 | A kind of functional form pig feed additive and preparation method thereof |
| CN108651735B (en) * | 2018-05-16 | 2022-02-01 | 大连三仪动物药品有限公司 | Peptide chelated iron composite preparation for improving production performance of sows and piglets |
| CN109329607A (en) * | 2018-11-07 | 2019-02-15 | 上海交通大学 | A kind of small peptide microelement chelate combination agent improving piglet oxidation resistance |
| CN113678948A (en) * | 2021-08-31 | 2021-11-23 | 广州康瑞德饲料科技有限公司 | Preparation method of self-assembled small peptide chelated iron for feed additive |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101606715A (en) * | 2008-06-17 | 2009-12-23 | 浙江科技学院 | A kind of preparation method who disturbs anionic food-grade amino acid ferrous chelate compound that do not contain |
| CN102093467B (en) * | 2010-11-02 | 2013-12-11 | 中国科学院亚热带农业生态研究所 | Dipeptide iron chelate for feed additives and preparation method thereof |
| CN102860512B (en) * | 2012-10-10 | 2014-07-02 | 中国食品发酵工业研究院 | Preparation method of black-bone chicken peptide and iron chelated biological iron supplement |
-
2013
- 2013-05-06 CN CN201310142525.4A patent/CN103224546B/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| CN103224546A (en) | 2013-07-31 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103224546B (en) | Hydrated carbamyl glycine dipeptide chelated iron and preparation method thereof | |
| CN102132782B (en) | Micropterus salmoides compound feed and preparation method thereof | |
| CN101731451B (en) | Compound mouldproof agent with acidizing effect and preparation method thereof | |
| CN103224461B (en) | Preparation method and application of large-particle crystal form methionine metal chelate | |
| CN103340293B (en) | Use of alpha-ketoglutaric acid | |
| CN101785534B (en) | Application of zinc glutamate and derivatives thereof as animal feed additive for promoting growth | |
| EP3670493B1 (en) | N,n-dimethylglycine organic acid compound salt and composition and application thereof | |
| EP3674286B1 (en) | Conjugate acid salt of n,n-dimethylglycine and organic acid, and composition and application thereof | |
| EP2680710B1 (en) | Enhanced bioavailable iodine molecules | |
| CN102603778A (en) | N-carbamyl-L-glutamic zinc complex and preparation method of N-carbamyl-L-glutamic zinc complex | |
| JP6179906B2 (en) | Use of dietary paranitroamino derivatives to reduce methane emissions in ruminants | |
| CN101735089A (en) | Method for synthesizing iron amino acid chelates by utilizing carbonyl iron | |
| CN104719631A (en) | Special vitamin nutrition agent for seawater industrial culture of Atlantic salmon, and preparation method thereof | |
| CN101289411B (en) | Method for preparing zinc threonine | |
| CN102516108A (en) | Formula for preparing ferrous bisglycinate and method thereof | |
| CN112409200B (en) | Preparation method and application of isoleucine chelate metal | |
| CN112293577A (en) | Fully-soluble ferrous glycinate and preparation method and application thereof | |
| JP6227762B2 (en) | Use of ethylenediamine metal complexes to deliver superabsorbent metals for animal nutrition | |
| CN103497133B (en) | Method for preparing N-methylol group-D,L-calcium methionine microelement chelates by means of saponification liquid produced through D,L- methionine | |
| CN113729121A (en) | Feed additive containing zinc benzoate and production process and application thereof | |
| CN109349221B (en) | Method for determining energy and protein demand of Tibet white cashmere goats in growth period | |
| CN102718803A (en) | Preparation method and application of N-carbamoyl-L-chromium glutamate complex | |
| CN102718685A (en) | Preparation method and application of N-carbamyl-L-glutamic acid manganese complex | |
| CN103431201A (en) | Threonine-free dietary premix for growing pigs, and use method thereof | |
| CN104472889A (en) | Special compound trace element nutrient for industrial mariculture of epinephelus malabaricus and preparation process thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant |