CN103211974B - Medicinal liquor for improving immunity and application thereof - Google Patents
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Abstract
The invention provides medicinal liquor for improving immunity. The medicinal liquor is obtained by soaking the following raw materials in parts by weight in grain liquor: 1-20 parts of codonopsis pilosula, 1-20 parts of cornua cervi pantotrichum, 1-20 parts of astragalus mongholicus, 1-20 parts of pseudo-ginseng, 1-20 parts of rehmannia glutinosa, 1-10 parts of cordyceps sinensis, 1-10 parts of yam, 1-10 parts of medlar, 1-10 parts of ginseng, 1-10 parts of carthamus tinctorius, 1-10 parts of eucommia ulmoides, 1-10 parts of lucid ganoderma, 1-10 parts of gecko, 1-10 parts of red date, 1-10 parts of sea horse, 1-10 parts of cistanche, 1-10 parts of herba epimedii, 1-10 parts of radix polygonati officinalis and the like. The medicinal liquor has the advantages of remarkably improving the tumor weight inhibition ratio and the NK (Natural Killer) cell activity, reducing the TNF (tumor necrosis factor) alpha content in the serum and promoting the IFN (Interferon) gamma and IL-2 (interleukin-2) contents to rise, so that the medicinal liquor is capable of boosting the humoral immunity and cell immunity of the human body with remarkable effect; and at the same time, the medicinal liquor is obtained by soaking natural animal and plant ingredients as well as pure traditional Chinese medicine materials, so that the color of the liquor is natural amber, no toxic or side effects are caused and the preparation is convenient.
Description
Technical field
The present invention relates to a kind of medicated wine and the application thereof that improve immunocompetence, specifically, is a kind of Chinese patent medicine prepared for raw material with raw material food medicine base material.
Background technology
Immunologic function is the very important biological function of human body, can give rise to diseases and promote human senility under this function.Equally, tumor is also a kind ofly reduce the disease caused because of immunologic function, be that the cell of human organ tissue produces under the external and interior long term at harmful factor a kind of take cell hyperproliferation as the neoplasm of main feature.The physiological need of this neoplasm and afflicted organ has nothing to do, and its rule not according to normal organ grows, and loses Normocellular function, destroys the structure of original organ, and what have can transfer to other position, threat to life.In recent years, tumor incidence improves gradually, becomes study hotspot to the suppression of tumor and treatment.
The tumor of being born in body is attributed to lump in the abdomen category by the traditional Chinese medical science.Entangle its cause of disease, often solidifying with the stagnation of QI, blood stasis, expectorant, becomes silted up malicious, that void is lost etc. is relevant.For trouble tumor person, can also treat with medicated wine at certain phase, particularly to reconvalescent, comparatively suitable.Some have the Chinese medicine of antitumor action, as Nidus Vespae, Scorpio, Pseudobulbus cremastrae seu pleiones, Cutis Bufonis, Gekko Swinhonis, Radix Actinidiae Chinensis, Radix Kansui, Rhizoma Dioscoreae Bulbiferaes etc. make medicated wine through steeping in wine, have certain curative effect to Therapeutic cancer, and in addition, Chinese patent literature CN 201010106429.0, applying date 2010-02-01, disclose a kind of Chinese medicinal liquor for the treatment of tumor, by grain neutral spirit and blood circulation promoting and blood stasis dispelling, dispersing swelling and dissipating binds, activate non-viable non-apoptotic cell, strengthen immunologic function, defying age, anti-cancer and anticancer Chinese crude drug is brewed forms, wherein said Chinese crude drug and weight portion thereof are: Radix Semiaquilegiae 5-7 part, Rhizoma Dioscoreae Bulbiferae 3-5 part, Dioscorea bulbifera L. mores than zero sub-2-4 part, Rhizoma Cyperi 1-3 part, Cortex Eucommiae 3-5 part processed, Caulis Sargentodoxae 2-4 part, fragrant Caulis seu Radix Schisandrae Henryi 1-3 part, Ramulus Uncariae Cum Uncis 5-15 part, Radix Acanthopanacis Trifoliati 2-4 part, Rhizoma Acori Graminei 1-3 part, Radix Ampelopsis delavayanae 1-3 part and Cortex Araliae Chinensis 2-4 part, described Chinese liquor is the Chinese liquor that Oryza glutinosa is brewageed, and alcoholic strength is 38-45, is loaded by above-mentioned Chinese crude drug in altar, and sealing soaks 3-5 month, obtains medicated wine, be applicable to lymphoma after filtration, fibroma, mastadenoma, bone tumor, hemangioma, bony spur and epidermis can be palpable lump, Chinese patent literature CN 200710013426.0, the applying date: 2007-01-31, disclose a kind of medicated wine and compound method thereof of Therapeutic cancer, it mainly forms: Phellinus igniarius (L. ex Fr.) Quel. 3-15 part, Ganoderma 3-20 part, Bufo siccus 3-20 part, Agkistrodon halys wine 5-50 part, yellow wine 3000-5000 part, first the Agkistrodon halys that lives is soaked in more than 2 months obtained Agkistrodon halys wine in the brewing grains white spirit of more than 50 degree, again by Phellinus igniarius (L. ex Fr.) Quel., Ganoderma, Bufo siccus is dried and grinds foam and Agkistrodon halys wine and the comprehensive exchange of yellow wine and form, this medicated wine can blood circulation promoting and blood stasis dispelling, to stimulate the menstrual flow eliminating stagnation, counteracting toxic substances is drawn out pus by applying a plaster to the affected part and is had no side effect, the symptom of tumour patient is made to improve after taking, tumor progressively reduces and disappears, and can shift by Tumor suppression, strengthen the immunity of organisms of patient.But above-mentioned medicated wine is all without definite animal or its inhibitory action on tumor of clinical trial certificate and the impact on body immunity.Therefore, the medicated wine that determined curative effect improves immunocompetence is significantly needed badly.
Summary of the invention
The object of the invention is for deficiency of the prior art, a kind of medicated wine improving immunocompetence is provided.
Of the present invention again one object be that the purposes of above-mentioned medicated wine is provided.
For achieving the above object, the technical scheme that the present invention takes is:
Improve a medicated wine for immunocompetence, it is used by the crude drug of following weight portion that grain wine is brewed forms: Radix Codonopsis 1-20 part, Cornu Cervi Pantotrichum 1-20 part, Radix Astragali 1-20 part, Radix Notoginseng 1-20 part, Radix Rehmanniae Preparata 1-20 part, Cordyceps 1-10 part, Rhizoma Dioscoreae 1-10 part, Fructus Lycii 1-10 part, Radix Ginseng 1-10 part, Flos Carthami 1-10 part, Cortex Eucommiae 1-10 part, Ganoderma 1-10 part, Gecko 1-10 part, Fructus Jujubae 1-10 part, Hippocampus 1-10 part, Herba Cistanches 1-10 part, Herba Epimedii 1-10 part, Rhizoma Polygonati Odorati 1-10 part.
Preferably, described grain wine is 40-60 degree grain wine.
Preferred, described grain wine is 50 degree of grain wine.
Preferably, described raw material food medicine base material and the weight proportion of grain wine are 1: (5-7).
Preferred, described raw material food medicine base material and the weight proportion of grain wine are 1: 6.
Preferably, described medicated wine is eaten medicine base material by the raw material of following weight portion and is used that grain wine is brewed to be formed: Radix Codonopsis 10 parts, 10 parts, Cornu Cervi Pantotrichum, the Radix Astragali 10 parts, Radix Notoginseng 10 parts, 10 parts, Radix Rehmanniae Preparata, Cordyceps 5 parts, Rhizoma Dioscoreae 5 parts, Fructus Lycii 5 parts, Radix Ginseng 5 parts, 5 parts, Flos Carthami, the Cortex Eucommiae 5 parts, Ganoderma 5 parts, Gecko 5 parts, Fructus Jujubae 5 parts, Hippocampus 5 parts, Herba Cistanches 5 parts, Herba Epimedii 5 parts, Rhizoma Polygonati Odorati 5 parts.
For realizing above-mentioned second object, the technical scheme that the present invention takes is:
As above arbitrary described medicated wine improves the application in the medicine of immunocompetence in preparation;
As above arbitrary described medicated wine application of reducing TNF alpha content in serum in preparation and/or improve in the medicine of IFN γ and IL-2 content in serum.
It should be noted that, described Cornu Cervi Pantotrichum refers to the unossified and young horn with fine hair of the stag of Cervus nippon Temminck or Cervus elaphus linnaeus, and nature and flavor are sweet, salty, warm, can kidney-Yang invigorating, fill blood, beneficial marrow, bone and muscle strengthening; Described Flos Carthami formal name used at school Flos Carthami, pungent, warm, GUIXIN, Liver Channel, have promoting blood circulation to restore menstrual flow, effect of stasis-dispelling and pain-killing; Described grain wine refers to various grains such as Sorghum vulgare Pers., Semen Maydis, rice, Oryza glutinosa, Fructus Hordei Vulgaris, Semen Tritici aestivi, Semen setariae, Semen avenae nudaes for raw material, after saccharifying, fermentation, adopts the Chinese liquor of distillating method brew.
The invention has the advantages that:
1, through testing medicated wine of the present invention to S-180 implanted solid tumor growth and the impact on immune function of mice, and using injection along amine chlorine platinum as positive control, result shows, compared to blank group, use the mice of medicated wine gavage of the present invention, its NK cytoactive significantly raises, in serum, TNF alpha content declines, IFN γ, IL-2 content raises, and shows that medicated wine of the present invention can strengthen humoral immunization and the immunization of cell of mice, Be very effective;
2, form by pure Chinese medicine is brewed, have no side effect, patient compliance is good;
3, preparation method is easy, is convenient to implement.
Detailed description of the invention
Below detailed description of the invention provided by the invention is elaborated.
the preparation (one) of embodiment 1 medicated wine of the present invention
get Gecko, put to death, clear up gastral content to clean, then with Radix Codonopsis 1 part, 20 parts, Cornu Cervi Pantotrichum, the Radix Astragali 1 part, Radix Notoginseng 20 parts, 1 part, Radix Rehmanniae Preparata, Cordyceps 5 parts, Rhizoma Dioscoreae 10 parts, Fructus Lycii 1 part, Radix Ginseng 10 parts, 1 part, Flos Carthami, the Cortex Eucommiae 10 parts, Ganoderma 1 part, Fructus Jujubae 10 parts, Hippocampus 1 part, Herba Cistanches 10 parts, Herba Epimedii 1 part, Rhizoma Polygonati Odorati 10 parts etc., be put in sealing in 540 parts (5 times of crude drug gross weight) 50 degree of rice grain wine together to soak, front ten days, every day stirs once, stir weekly once after ten days, total immersion steeps two months, then leaves standstill five.
the preparation (two) of embodiment 2 medicated wine of the present invention
Get Gecko, put to death, clear up gastral content to clean, then with Radix Codonopsis 20 parts, 1 part, Cornu Cervi Pantotrichum, the Radix Astragali 20 parts, Radix Notoginseng 1 part, 20 parts, Radix Rehmanniae Preparata, Cordyceps 5 parts, Rhizoma Dioscoreae 1 part, Fructus Lycii 10 parts, Radix Ginseng 1 part, 10 parts, Flos Carthami, the Cortex Eucommiae 1 part, Ganoderma 10 parts, Fructus Jujubae 1 part, Hippocampus 10 parts, Herba Cistanches 1 part, Herba Epimedii 10 parts, Rhizoma Polygonati Odorati 1 part etc., be put in sealing in 826 parts (7 times of crude drug gross weight) 40 degree of Semen setariae grain wine together to soak, front ten days, every day stirs once, stir weekly once after ten days, total immersion steeps two months, then leaves standstill five.
the preparation (three) of embodiment 3 medicated wine of the present invention
Get Gecko, put to death, clear up gastral content to clean, then with Radix Codonopsis 1 part, 1 part, Cornu Cervi Pantotrichum, the Radix Astragali 20 parts, Radix Notoginseng 20 parts, 1 part, Radix Rehmanniae Preparata, Cordyceps 5 parts, Rhizoma Dioscoreae 1 part, Fructus Lycii 10 parts, Radix Ginseng 10 parts, 1 part, Flos Carthami, the Cortex Eucommiae 1 part, Ganoderma 10 parts, Fructus Jujubae 10 parts, Hippocampus 1 part, Herba Cistanches 1 part, Herba Epimedii 10 parts, Rhizoma Polygonati Odorati 10 parts etc., be put in sealing in 648 parts (6 times of crude drug gross weight) 60 degree of Sorghum vulgare Pers. grain wine together to soak, front ten days, every day stirs once, stir weekly once after ten days, total immersion steeps two months, then leaves standstill five.
the preparation (four) of embodiment 4 medicated wine of the present invention
Get Gecko, put to death, clear up gastral content to clean, then with Radix Codonopsis 20 parts, 20 parts, Cornu Cervi Pantotrichum, the Radix Astragali 1 part, Radix Notoginseng 1 part, 20 parts, Radix Rehmanniae Preparata, Cordyceps 5 parts, Rhizoma Dioscoreae 10 parts, Fructus Lycii 1 part, Radix Ginseng 1 part, 10 parts, Flos Carthami, the Cortex Eucommiae 10 parts, Ganoderma 1 part, Fructus Jujubae 1 part, Hippocampus 10 parts, Herba Cistanches 10 parts, Herba Epimedii 1 part, Rhizoma Polygonati Odorati 1 part etc., be put in sealing in 708 parts (6 times of crude drug gross weight) 40 degree of Oryza glutinosa grain wine together to soak, front ten days, every day stirs once, stir weekly once after ten days, total immersion steeps two months, then leaves standstill five.
the preparation (five) of embodiment 5 medicated wine of the present invention
Get Gecko, put to death, clear up gastral content to clean, then with Radix Codonopsis 1 part, 20 parts, Cornu Cervi Pantotrichum, the Radix Astragali 20 parts, Radix Notoginseng 1 part, 20 parts, Radix Rehmanniae Preparata, Cordyceps 5 parts, Rhizoma Dioscoreae 10 parts, Fructus Lycii 1 part, Radix Ginseng 10 parts, 10 parts, Flos Carthami, the Cortex Eucommiae 1 part, Ganoderma 10 parts, Fructus Jujubae 10 parts, Hippocampus 1 part, Herba Cistanches 10 parts, Herba Epimedii 10 parts, Rhizoma Polygonati Odorati 1 part etc., be put in sealing in 680 parts (5 times of crude drug gross weight) 50 degree of Fructus Hordei Vulgaris grain wine together to soak, front ten days, every day stirs once, stir weekly once after ten days, total immersion steeps two months, then leaves standstill five.
the preparation (six) of embodiment 6 medicated wine of the present invention
Get Gecko, put to death, clear up gastral content to clean, then with Radix Codonopsis 20 parts, 1 part, Cornu Cervi Pantotrichum, the Radix Astragali 1 part, Radix Notoginseng 20 parts, 1 part, Radix Rehmanniae Preparata, Cordyceps 5 parts, Rhizoma Dioscoreae 1 part, Fructus Lycii 10 parts, Radix Ginseng 1 part, 1 part, Flos Carthami, the Cortex Eucommiae 10 parts, Ganoderma 1 part, Fructus Jujubae 1 part, Hippocampus 10 parts, Herba Cistanches 1 part, Herba Epimedii 1 part, Rhizoma Polygonati Odorati 10 parts etc., be put in sealing in 630 parts (7 times of crude drug gross weight) 60 degree of Semen Tritici aestivi grain wine together to soak, front ten days, every day stirs once, stir weekly once after ten days, total immersion steeps two months, then leaves standstill five.
the preparation (seven) of embodiment 7 medicated wine of the present invention
Get Gecko, put to death, clear up gastral content to clean, then with Radix Codonopsis 1 part, 20 parts, Cornu Cervi Pantotrichum, the Radix Astragali 20 parts, Radix Notoginseng 20 parts, 1 part, Radix Rehmanniae Preparata, Cordyceps 5 parts, Rhizoma Dioscoreae 10 parts, Fructus Lycii 10 parts, Radix Ginseng 10 parts, 1 part, Flos Carthami, the Cortex Eucommiae 10 parts, Ganoderma 10 parts, Fructus Jujubae 10 parts, Hippocampus 1 part, Herba Cistanches 10 parts, Herba Epimedii 10 parts, Rhizoma Polygonati Odorati 1 part etc., be put in sealing in 870 parts (6 times of crude drug gross weight) 50 degree of rice grain wine together to soak, front ten days, every day stirs once, stir weekly once after ten days, total immersion steeps two months, then five are left standstill.
the preparation (eight) of embodiment 8 medicated wine of the present invention
Get Gecko, put to death, clear up gastral content to clean, then with Radix Codonopsis 1 part, 1 part, Cornu Cervi Pantotrichum, the Radix Astragali 1 part, Radix Notoginseng 1 part, 10 parts, Radix Rehmanniae Preparata, Cordyceps 5 parts, Rhizoma Dioscoreae 10 parts, Fructus Lycii 5 parts, Radix Ginseng 5 parts, 10 parts, Flos Carthami, the Cortex Eucommiae 1 part, Ganoderma 1 part, Fructus Jujubae 5 parts, Hippocampus 10 parts, Herba Cistanches 1 part, Herba Epimedii 10 parts, Rhizoma Polygonati Odorati 5 parts etc., be put in sealing in 385 parts (5 times of crude drug gross weight) 50 degree of Semen avenae nudae grain wine together to soak, front ten days, every day stirs once, stir weekly once after ten days, total immersion steeps two months, then leaves standstill five.
the preparation (nine) of embodiment 9 medicated wine of the present invention
Get Gecko, put to death, clear up gastral content to clean, then with Radix Codonopsis 10 parts, 20 parts, Cornu Cervi Pantotrichum, the Radix Astragali 1 part, Radix Notoginseng 20 parts, 20 parts, Radix Rehmanniae Preparata, Cordyceps 5 parts, Rhizoma Dioscoreae 10 parts, Fructus Lycii 5 parts, Radix Ginseng 1 part, 5 parts, Flos Carthami, the Cortex Eucommiae 10 parts, Ganoderma 1 part, Fructus Jujubae 10 parts, Hippocampus 10 parts, Herba Cistanches 1 part, Herba Epimedii 1 part, Rhizoma Polygonati Odorati 1 part etc., be put in sealing in 882 parts (7 times of crude drug gross weight) 40 degree of rice grain wine together to soak, front ten days, every day stirs once, stir weekly once after ten days, total immersion steeps two months, then leaves standstill five.
the preparation (ten) of embodiment 10 medicated wine of the present invention
Get Gecko, put to death, clear up gastral content to clean, then with Radix Codonopsis 10 parts, 10 parts, Cornu Cervi Pantotrichum, the Radix Astragali 10 parts, Radix Notoginseng 10 parts, 10 parts, Radix Rehmanniae Preparata, Cordyceps 5 parts, Rhizoma Dioscoreae 5 parts, Fructus Lycii 5 parts, Radix Ginseng 5 parts, 5 parts, Flos Carthami, the Cortex Eucommiae 5 parts, Ganoderma 5 parts, Fructus Jujubae 5 parts, Hippocampus 5 parts, Herba Cistanches 5 parts, Herba Epimedii 5 parts, Rhizoma Polygonati Odorati 5 parts etc., be put in sealing in 630 parts (6 times of crude drug gross weight) 50 degree of rice grain wine together to soak, front ten days, every day stirs once, stir weekly once after ten days, total immersion steeps two months, then leaves standstill five.
It should be noted that, the color of the rice wine that the present invention prepares is natural amber color (brownish red).
the zoopery of embodiment 11 medicated wine of the present invention
1 experiment material
Test medicine: medicated wine prepared by embodiment 10.
Positive control medicine: injection is along amine chlorine platinum, and production unit: Qilu Pharmaceutical Factory, lot number: the accurate word 20023461 of traditional Chinese medicines, character: glassy yellow or orange-yellow crystalline powder, odorless, prepares: normal saline is made into 1mg/kg.
Laboratory animal: strain: Kun Ming mice, source: Shanghai Slac Experimental Animal Co., Ltd., the quality certification number: SCXK(Shanghai) 2009-0019, body weight: 20 ± 2 grams, sex: male, 6 week age.
Experimental cell: transplanted tumor S-180 cell strain is that pharmaceutcal corporation, Ltd of Hutchison China Trade Holdings goes down to posterity maintenance; YAC-1 cell is purchased from cell institute of Chinese Academy of Sciences cell bank.
INF γ, TNF α, IL-2 Elisa test kit: be purchased from Wuhan doctor's moral company (China, Wuhan).
2 experimental techniques
2.1 eliminating evil researchs (impact on mice S-180 solid tumor)
Modeling: the well-grown ascites of aseptic aspiration is 15ml altogether, and cell concentration is about 10
7/ ml, every mice axil subcutaneous vaccination 0.2ml.
Grouping: the mice after modeling is divided into five groups at random, is respectively: dosage group (20g crude drug/kg), medicated wine high dose group (40g crude drug/kg) in blank group, suitable amine chlorine platinum group, medicated wine low dose group (10g crude drug/kg), medicated wine.
Administering mode: administration next day after inoculation, medicated wine high dose group is daily 2 times, other groups daily 1 time.In medicated wine high dose group, medicated wine, dosage group, each administration volume of medicated wine low dose group are 0.5ml/20g body weight, continuous gavage 7 days.Dosage along amine chlorine platinum group is 8mg/kg, carries out lumbar injection 0.3ml, at inoculation the 1st, 3,5 days lumbar injections.Blank group uses normal saline gavage every day once.Inoculate latter 14 days de-necks to put to death, solution takes tumor block, scale tumor weight, and result judges according to following formula: tumor control rate (%)=[(matched group average tumor weight-administration group average tumor weight) the average tumor weight of/matched group] × 100%.
2.2 NK cytoactive detections
2.2.1 the preparation of target cell:
Get well-grown YAC-1 cell centrifugal with 1000rpm, 5min after, with being 1 × 10 containing the RPMI1640 complete medium of 10% calf serum by its furnishing concentration
5the cell suspension of/ml is stand-by.
2.2.2 the preparation of effector lymphocyte:
After being put to death by mice cervical dislocation, asepticly win spleen, divide into groups the RPMI-1640 be placed in containing 10% calf serum, soaks, after 20 minutes, spleen is cut into 2-3mm
2, the grinding of glass syringe nook closing member is broken, with the sieved filter of 200 order rustless steel cell, after cell suspension is centrifugal with 1000rpm, 10min, is 2 × 10 by the RPMI1640 culture medium containing 10% calf serum by cell furnishing concentration
6the cell suspension of/ml is stand-by.
2.2.3 mtt assay measures NK cells in mice activity:
If blank group (100 μ l culture medium), experimental group (50 μ l splenocyte+50 μ l YAC-1 cell), effector lymphocyte's matched group (50 μ l splenocyte+50 μ l culture medium), target cell matched group (50 μ l YAC-1 cell+50 μ l culture medium), cell is added in 96 orifice plates, Tissue Culture Plate is put into 37 degree of 5% CO
2cell culture incubator reacts 4 hours, adds 5mg/ml MTT 10 μ l, Tissue Culture Plate is put into 37 degree of 5% CO
2cell culture incubator reacts 4 hours, centrifugal segregation supernatant, adds DMSO solution 150 μ l, measures OD
492value.The actual OD value in each hole=this hole actual measurement OD value-blank well OD value.
According to formulae discovery NK cytoactive: NK cytoactive=[target cell control wells OD value-(experimental port OD value-imitate cell control well OD value)]/target cell control wells OD value × 100%.
The mensuration of 2.3 tumor-bearing mice serum TNF alpha contents
The mensuration of TNF alpha content is with reference to mice TNF α Elisa-Kit description program, and every hole adds 100 μ l standard substance or testing samples, according to O.D
450drawing standard curve, detects cytokine content in test serum.
The mensuration of 2.4 tumor-bearing mice serum I FN γ content
The mensuration of IFN-γ content is with reference to mice IFN-γ Elisa-Kit description program, and every hole adds 100 μ l standard substance or testing samples, according to O.D
450drawing standard curve, detects cytokine content in test serum.
The mensuration of 2.5 tumor-bearing mice soluble Interleukin-2s
The reference mice IL-2 Elisa-Kit description program of IL-2 assay, every hole adds 100 μ l standard substance or testing samples, according to O.D
450drawing standard curve, detects cytokine content in test serum.
2.6 statistical disposition
Result with means standard deviation (
± s) represent; The significance of difference is matched
tinspection.
3 result of the tests
The heavy inhibitory action of tumor of 3.1 pairs of S-180 solid tumors
Medicated wine of the present invention at dosage 10g, during 20g, 40g crude drug/kg, to the tumour inhibiting rate result of the test of S-180 solid tumor in table 1.
Table 1 medicated wine of the present invention to the tumor-like hyperplasia of tumor-bearing mice (
± s)
Note: compare with blank group, *
p<0.05.
2.2 impacts on S-180 solid tumor NK cytoactive
Medicated wine of the present invention at dosage 10g, during 20g, 40g crude drug/kg, on the impact of the NK cytoactive of S-180 solid tumor in table 2.
Table 2 medicated wine of the present invention is on the impact of tumor-bearing mice NK cytoactive
Note: compare with blank group, *
p<0.05.
3.3 impacts on mice S-180 solid tumor serum TNF alpha content
Medicated wine of the present invention at dosage 10g, during 20g, 40g crude drug/kg, on the impact of the serum TNF alpha content of S-180 solid tumor in table 3.
Table 3 medicated wine of the present invention is on the impact (pg/ml) of tumor-bearing mice serum TNF alpha content
Note: compared with blank group, *
p<0.05, * *
p<0.01.
3.4 impacts on mice S-180 solid tumor serum I FN γ content
Medicated wine of the present invention at dosage 10g, during 20g, 40g crude drug/kg, on the impact of S-180 solid tumor serum I FN γ content in table 4.
Table 4 medicated wine of the present invention is on the impact (pg/ml) of tumor-bearing mice serum I FN γ content
Note: compare with blank group, *
p<0.05.
3.5 impacts on mice S-180 solid tumor soluble Interleukin-2
Medicated wine of the present invention at dosage 10g, during 20g, 40g crude drug/kg, on the impact of S-180 solid tumor soluble Interleukin-2 in table 5.
Table 5 medicated wine of the present invention is on the impact (pg/ml) of tumor-bearing mice soluble Interleukin-2
Note: compare with blank group, *
p<0.05.
Above experimental result shows, medicated wine of the present invention is demonstrating inhibitory action simultaneously to mice S-180 solid tumor, demonstrate the humoral immunization and immunization of cell that strengthen mice.This experiment is pointed out, and medicated wine of the present invention is relevant with its immunity moderation function.
The above is only the preferred embodiment of the present invention; it should be pointed out that for those skilled in the art, under the prerequisite not departing from the inventive method; can also make some improvement and supplement, these improve and supplement and also should be considered as protection scope of the present invention.
Claims (7)
1. one kind is improved the medicated wine of immunocompetence, it is characterized in that, it forms by the raw material food medicine base material grain wine of following weight portion is brewed: Radix Codonopsis 10 parts, 10 parts, Cornu Cervi Pantotrichum, the Radix Astragali 10 parts, Radix Notoginseng 10 parts, 10 parts, Radix Rehmanniae Preparata, Cordyceps 5 parts, Rhizoma Dioscoreae 5 parts, Fructus Lycii 5 parts, Radix Ginseng 5 parts, 5 parts, Flos Carthami, the Cortex Eucommiae 5 parts, Ganoderma 5 parts, Gecko 5 parts, Fructus Jujubae 5 parts, Hippocampus 5 parts, Herba Cistanches 5 parts, Herba Epimedii 5 parts, Rhizoma Polygonati Odorati 5 parts.
2. medicated wine according to claim 1, is characterized in that, described grain wine is 40-60 degree grain wine.
3. medicated wine according to claim 2, is characterized in that, described grain wine is 50 degree of grain wine.
4. medicated wine according to claim 1, is characterized in that, described raw material food medicine base material and the weight proportion of grain wine are 1: (5-7).
5. medicated wine according to claim 4, is characterized in that, described raw material food medicine base material and the weight proportion of grain wine are 1: 6.
6. the arbitrary described medicated wine of claim 1-5 improves the application in the medicine of immunocompetence in preparation.
7. the arbitrary described medicated wine of the claim 1-5 application that reduces TNF alpha content in serum in preparation and/or improve in the medicine of IFN γ and IL-2 content in serum.
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| CN103656121A (en) * | 2013-12-06 | 2014-03-26 | 山东中大药业有限公司 | Immunity-improving traditional Chinese medicine composition and preparation method thereof |
| CN105596548A (en) * | 2016-01-26 | 2016-05-25 | 赵建国 | Health care wine based on black Chinese wolfberry and preparation method of heath care wine |
| CN106237121A (en) * | 2016-08-31 | 2016-12-21 | 贵州省喜春源生态农业有限公司 | A kind of medicated wine improving immunity |
| CN106474344A (en) * | 2016-12-21 | 2017-03-08 | 神农架林区中医医院 | Medicinal liquor |
| CN109350696A (en) * | 2018-11-27 | 2019-02-19 | 刘忠明 | A kind of Chinese medicine preparation and preparation method thereof assisting in the treatment of coronary heart disease |
| CN109589374A (en) * | 2019-01-11 | 2019-04-09 | 陕西稻香网络科技有限公司 | A kind of multi-functional healthy medicated wine and its brewing method |
| CN110373298A (en) * | 2019-08-20 | 2019-10-25 | 山西省农业科学院食用菌研究所 | The production method of ganoderma lucidum stilbene ginseng series of products |
| CN111773374A (en) * | 2020-07-07 | 2020-10-16 | 深圳市清大生物科技有限公司 | Formula of sealwort oyster peptide for improving immunity |
| CN111991549A (en) * | 2020-09-30 | 2020-11-27 | 四川省大生堂药品开发有限公司 | Traditional Chinese medicine composition for improving immunity and preparation method thereof |
| CN113559152B (en) * | 2021-09-27 | 2021-12-14 | 北京本草源生物科技有限公司 | Liver-protecting traditional Chinese medicine composition and beverage as well as preparation method and application thereof |
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| CN100457158C (en) * | 2005-04-18 | 2009-02-04 | 潘文祥 | Medicine for fortifying human body immune function |
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