CN103203046A - blood bag system for decreasing collection damage and matched blood collection method - Google Patents
blood bag system for decreasing collection damage and matched blood collection method Download PDFInfo
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- CN103203046A CN103203046A CN2013100531612A CN201310053161A CN103203046A CN 103203046 A CN103203046 A CN 103203046A CN 2013100531612 A CN2013100531612 A CN 2013100531612A CN 201310053161 A CN201310053161 A CN 201310053161A CN 103203046 A CN103203046 A CN 103203046A
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Abstract
The invention provides a blood bag system for effectively decreasing 'collection damage' in whole blood collection and preparing blood components by the principle of 'collecting blood by proportionally adding preservative solution in a following manner', and a blood collection method matched with the system. By the system and the method, high-quality whole blood can be manually collected, high-quality blood products are prepared by separation on the basis, blood quality can be positively improved, and blood transfusion safety can be positively ensured. The method includes the steps: applying the blood bag system for manually collecting the whole blood by the principle of 'proportionally adding the preservative solution in the following manner' and preparing the blood components; and establishing a whole blood collection method suitable for the system.
Description
Technical field
The present invention relates to medical domain, particularly relate to blood collecting and supplying field whole blood gather employed, as can effectively to reduce " gathering damage " equipment special and with the blood collection method of its coupling.
Background technology
The blood that the blood donor contributes must mix with contained an amount of Precerving liquid in the blood taking bag, can guarantee that blood its physical and chemical index in the storage life of stipulating of gathering meets relevant prescription with function.Yet, conventional gravity blood collection method is used in the collection of whole blood at present, the blood that is the blood donor dropwise enters in the blood bag that adds the fixed amount Precerving liquid in advance by conduit, therefore, in the whole blood collection procedure before stopping to take a blood sample, blood volume in the blood bag all can't keep correct ratio with blood Precerving liquid capacity, cause especially at the collection initial stage, the blood of gathering and the ratio of Precerving liquid are high imbalance state, make the about 30ml blood that enters the blood bag at first, be subjected to the effect of acid Precerving liquid, cause erythrocytic membrane structure and physical and chemical index to change, cause erythrocytic damage, this is erythrocytic " gathering damage ".
" gathering damage " in the blood collection procedure and storage process cause erythrocyte that a series of biochemistrys and structural change " storing damage " takes place, the two all can influence erythrocytic membrane structure, energy metabolism and physiological function, the final blood quality that influences in the storage life, back erythrocyte existence and changing function cause transfusing blood.A large amount of research datas show that the residing low pH of erythrocyte is the key factor of erythrocyte damage, and the whole blood volume in the blood bag has only the ratio correct with the maintenance of blood Precerving liquid capacity, can guarantee that just erythrocytic storage condition pH is in proper level.Domestic and international normally used whole blood Precerving liquid ACD-B, CPD and CPDA-1, the pH value of himself be respectively 5.0,5.6 and the pH value of 5.6(normal human inner blood be 7.4), they mix the final proper ratio in back and are respectively 1:4,1:7 and 1:7 with blood, can guarantee that a whole blood of gathering and storing is at the prescription that stores whole latter stage.
Although can reduce erythrocytic collection damage by the method for continuous concussion blood bag, abundant mixing in the conventional blood collection method, but in the whole blood collection procedure before stopping to take a blood sample, blood and Precerving liquid all can't keep proper proportion all the time in the blood bag, therefore, if can adopt in proportion and gather blood with adding the Precerving liquid blood collection method, to make that blood and Precerving liquid remain proper proportion in the blood bag, can reduce " gathering damage " greatly to the influence of erythrocytic membrane structure, energy metabolism and physiological function, to improving blood quality, guaranteeing that transfusion safety has positive meaning.
The invention provides a kind of blood bag system and supporting blood-sampling method with it, the principle of this system and concrete grammar utilization " in proportion with adding the Precerving liquid blood sampling " can effectively reduce the generation of " gathering damage " when gathering whole blood.
Summary of the invention
The invention provides that a kind of effective minimizing " gather damage " takes place and and carry out the blood bag system of blood constituent preparation and the blood-sampling method that foundation matches, utilize this system and method to gather high-quality whole blood and separate and prepare high-quality Blood Preparations.
Method of the present invention may further comprise the steps: the blood bag system that " in proportion with adding Precerving liquid " principle is gathered whole blood and carried out the blood constituent preparation is used in invention, sets up the whole blood acquisition method that is suitable for this system.
Now at technical specification of the present invention and method, details are as follows respectively:
1. the blood bag system that a minimizing " is gathered damage "
The properties of product structure of the blood bag system of a kind of minimizing " gather damage " and consisting of: blood taking needle, flexible pipe, sealed mouth, intermediate plate, threeway, blood taking bag, flow regulator, pre-mixing apparatus, blood filter, leucocyte-removing filter, the bag that keeps sample, transfering bag, storage blood bag, blood Precerving liquid bag and the alserver's solution bag of rolling, see accompanying drawing for details.
2. the method that the blood bag system that uses a kind of minimizing " to gather damage " is gathered whole blood
(1). check the blood bag system, must confirm that each component of blood bag system do not have breakage, ne-leakage, pollution-free, pipeline unfolded, anticoagulant and Precerving liquid do not have variable color and preserve liquid and no foreign body, protect the pin medicated cap and do not have and come off, and can use before the deadline
(2). set the blood sampling parameter of effect according to the labelled amount of blood bag, routinely the blood donor is carried out after identity checks, links up and assess, carry out disinfection and puncture, the puncture front opening is at the flow regulator of opening between Precerving liquid bag (10) and the pre-mixing apparatus (8) (11), 3-5 is dripped after Precerving liquid splashes into pre-mixing apparatus, close flow regulator
(3). after the venipuncture success, if use the keep sample blood taking bag of bag of band, then unclamp the bag clips that keeps sample (6) earlier, treat that about 15～20 ml blood that are used for blood testing that flow out at first flow into the bag that keeps sample after, folder closes the bag clips that keeps sample again; If use not with the blood taking bag of the bag that keeps sample, then unclamp obstruction piece lower end flow-stopping clip (7), make blood flow directly into pre-mixing apparatus, keep the drop of venipuncture point and blood bag, keep blood flow unobstructed
(4). utilize flow regulator dropwise to go into the speed of pre-mixing apparatus according to the kind of Precerving liquid control Precerving liquid, making the blood that enters pre-mixing apparatus and the ratio of Precerving liquid is 1:4(ACD-B) or 1:7(CPD and CPDA-1) about
(5). adopt and mix hemostix or manual blood sampling (should mix 1 time in per at least 90 seconds) continuously, make to flow into the abundant mixing of blood taking bag (13) blood; When the blood volume in the blood taking bag reaches requirement, advise blood donor pine fist, unclamp tourniquet, clamp the needle handle rear tube with mosquito forceps (folder), press the point of puncture top with sterilized cotton ball, dial except syringe needle, press for a moment hemorrhage to not having
(6). after blood sampling finishes, if still leave a small amount of Precerving liquid in Precerving liquid bag and the pipeline, then with blood bag clamp specially Precerving liquid is clamp-oned in the blood taking bag, finish simultaneously keep sample, heat seal, labeling, record and checking work.
(7). utilize multi-joint bag technique, according to different centrifugal rotational speed, time and temperature, under suitable condition, carry out the preparation of blood constituent.
The present invention's said " blood bag system " refers to that blood taking bag and the transfering bag of being made up of blood taking needle, take a blood sample conduit and the solvent that contains an amount of anticoagulant of being made up of conduit and several plastic containers, keep sample bag (having/does not have), defecator and pre-mixing apparatus form jointly is used for preparing separating of blood sampling and blood constituent the hermetically sealed plastic containers system of preservation.
The present invention's said " blood bag " refers to for blood collection, keeps sample, the general name of the plastic containers of separation, storage, transportation and infusion.
The present invention's said " blood taking bag " refers to be made up of blood taking needle, the container of taking a blood sample conduit and containing an amount of anticoagulant, is used for the hermetically sealed plastic containers of blood sampling.
The present invention's said " transfering bag " is made up of conduit and plastic containers, is used for the separation preparation of blood constituent, the hermetically sealed plastic containers of preserving.
The present invention's said " bag keeps sample " is made up of conduit and plastic containers, is used for the hermetically sealed plastic containers that obtain and retain of blood preparation.
The present invention's said " whole blood " refers to adopt the blood that includes an amount of Precerving liquid and various blood constituents into the blood bag.
The present invention's said " Precerving liquid " refers to anticoagulant, glucose etc. to be the class medicament prevent blood coagulation, to keep various composition activities and physiological function in the blood of being used for of main component.
The present invention's said " ACD-B liquid " refers to blood anticoagulant and preserves with compound recipe sodium citrate liquid (citric acid, sodium citrate and glucose).
The present invention's said " CPD liquid " refers to blood anticoagulant and preserves with the solution that contains citric acid, sodium citrate, sodium dihydrogen phosphate and glucose.
The present invention's said " CPDA-1 liquid " refers to blood anticoagulant and preserves with the solution that contains citric acid, sodium citrate, sodium dihydrogen phosphate, glucose and adenine.
The present invention's said " alserver's solution " refers to add in order to keep erythrocyte one class medicament active and physiological function, as MAP, SAGM, CPDA-1, and AS-1, AS-3, AS-5 and 0.9% sodium chloride solution etc.
Description of drawings
Accompanying drawing 1: blood bag system (bigeminy bag or three bags) structural representation
1: blood taking needle, 2: threeway, 3: flexible pipe, 4: bag keeps sample, 5: bag interface keeps sample, 6: the sackholder sheet keeps sample, 7: blood sampling path flexible pipe intermediate plate, 8: pre-mixing apparatus, 9: roll mouth, 10: blood Precerving liquid bag, 11: flow regulator, 12: blood filter, 3: blood taking bag, 14: do not remove white path flexible pipe intermediate plate, 15: the leucocyte-removing filter, 16: leucocyte-removing filter path intermediate plate, 17: the whole blood transfering bag, 18: blood plasma transfering bag intermediate plate, 19: alserver's solution path flexible pipe intermediate plate, 20: the alserver's solution bag, 21: the blood plasma transfering bag
Accompanying drawing 2: blood bag system (three bags or tetrad bag) structural representation
1: blood taking needle, 2: threeway, 3: flexible pipe, 4: bag keeps sample, 5: bag interface keeps sample, 6: the sackholder sheet keeps sample, 7: blood sampling path flexible pipe intermediate plate, 8: pre-mixing apparatus, 9: roll mouth, 10: blood Precerving liquid bag, 11: flow regulator, 12: blood filter, 13: blood taking bag, 14: do not remove white path flexible pipe intermediate plate, 15: the leucocyte-removing filter, 16: leucocyte-removing filter path intermediate plate, 17: the whole blood transfering bag, 18: blood plasma transfering bag intermediate plate, 19: alserver's solution path flexible pipe intermediate plate, 20: the alserver's solution bag, 21: the blood plasma transfering bag, 22: transfering bag, 23: the transfering bag intermediate plate.
The specific embodiment
Abide by " China's blood transfusion regulations for technical operation " (2012 editions), State Standard of the People's Republic of China GB18469-2012 and " whole blood and component blood prescription " during the present invention uses and separate and prepare, operate by preceding method.
Embodiment 1 gathers the 200ml whole blood of ACD-B anticoagulant
(1). check multi-joint bag (bigeminy bag, three bags or tetrad bag) blood bag system, each component of affirmation blood bag system does not have breakage, ne-leakage, and is pollution-free, the pipeline unfolded, anticoagulant and Precerving liquid do not have variable color and preserve liquid and no foreign body, and protect the pin medicated cap and do not have and come off, and before the deadline
(2). set the blood sampling parameter of effect according to the labelled amount of blood bag, routinely the blood donor is carried out after identity checks, links up and assess, carry out disinfection and puncture, the puncture front opening is at the flow regulator of opening between Precerving liquid bag and the pre-mixing apparatus, 3-5 is dripped after Precerving liquid splashes into pre-mixing apparatus, close flow regulator
(3). after the venipuncture success, if use the keep sample blood taking bag of bag of band, then unclamp the bag clips that keeps sample earlier, treat that about 15～20 ml blood that are used for blood testing that flow out at first flow into the bag that keeps sample after, folder closes the bag clips that keeps sample again; If use not with the blood taking bag of the bag that keeps sample, then unclamp obstruction piece lower end flow-stopping clip, make blood flow directly into pre-mixing apparatus, keep the drop of venipuncture point and blood bag, keep blood flow unobstructed
(4). utilize flow regulator dropwise to go into the speed of pre-mixing apparatus according to the kind of Precerving liquid control Precerving liquid, making the blood that enters pre-mixing apparatus and the ratio of Precerving liquid is about 1:4
(5). adopt and mix hemostix or manual blood sampling (should mix 1 time in per at least 90 seconds) continuously, make to flow into the abundant mixing of blood taking bag blood; When the blood volume in the blood taking bag reaches requirement, advise blood donor pine fist, unclamp tourniquet, clamp the needle handle rear tube with mosquito forceps (folder), press the point of puncture top with sterilized cotton ball, dial except syringe needle, press for a moment hemorrhage to not having
(6). after blood sampling finishes, if still leave a small amount of Precerving liquid in Precerving liquid bag and the pipeline, then with blood bag clamp specially Precerving liquid is clamp-oned in the blood taking bag, finish simultaneously keep sample, heat seal, labeling, record and checking work.
(7). utilize multi-joint bag technique, according to different centrifugal rotational speed, time and temperature, under suitable condition, carry out the preparation of blood constituent.
Embodiment 2 gathers the 300ml whole blood of CPD anticoagulant
(1). check multi-joint bag (bigeminy bag, three bags or tetrad bag) blood bag system, each component of affirmation blood bag system does not have breakage, ne-leakage, and is pollution-free, the pipeline unfolded, anticoagulant and Precerving liquid do not have variable color and preserve liquid and no foreign body, and protect the pin medicated cap and do not have and come off, and before the deadline
(2). set the blood sampling parameter of effect according to the labelled amount of blood bag, routinely the blood donor is carried out after identity checks, links up and assess, carry out disinfection and puncture, the puncture front opening is at the flow regulator of opening between Precerving liquid bag and the pre-mixing apparatus, 3-5 is dripped after Precerving liquid splashes into pre-mixing apparatus, close flow regulator
(3). after the venipuncture success, if use the keep sample blood taking bag of bag of band, then unclamp the bag clips that keeps sample earlier, treat that about 15～20 ml blood that are used for blood testing that flow out at first flow into the bag that keeps sample after, folder closes the bag clips that keeps sample again; If use not with the blood taking bag of the bag that keeps sample, then unclamp obstruction piece lower end flow-stopping clip, make blood flow directly into pre-mixing apparatus, keep the drop of venipuncture point and blood bag, keep blood flow unobstructed
(4). utilize flow regulator dropwise to go into the speed of pre-mixing apparatus according to the kind of Precerving liquid control Precerving liquid, making the blood that enters pre-mixing apparatus and the ratio of Precerving liquid is about 1:7
(5). adopt and mix hemostix or manual blood sampling (should mix 1 time in per at least 90 seconds) continuously, make to flow into the abundant mixing of blood taking bag blood; When the blood volume in the blood taking bag reaches requirement, advise blood donor pine fist, unclamp tourniquet, clamp the needle handle rear tube with mosquito forceps (folder), press the point of puncture top with sterilized cotton ball, dial except syringe needle, press for a moment hemorrhage to not having
(6). after blood sampling finishes, if still leave a small amount of Precerving liquid in Precerving liquid bag and the pipeline, then with blood bag clamp specially Precerving liquid is clamp-oned in the blood taking bag, finish simultaneously keep sample, heat seal, labeling, record and checking work.
(7). utilize multi-joint bag technique, according to different centrifugal rotational speed, time and temperature, under suitable condition, carry out the preparation of blood constituent.
Embodiment 3 gathers the 400ml whole blood of CPDA-1 anticoagulant
(1). check multi-joint bag (bigeminy bag, three bags or tetrad bag) blood bag system, each component of affirmation blood bag system does not have breakage, ne-leakage, and is pollution-free, the pipeline unfolded, anticoagulant and Precerving liquid do not have variable color and preserve liquid and no foreign body, and protect the pin medicated cap and do not have and come off, and before the deadline
(2). set the blood sampling parameter of effect according to the labelled amount of blood bag, routinely the blood donor is carried out after identity checks, links up and assess, carry out disinfection and puncture, the puncture front opening is at the flow regulator of opening between Precerving liquid bag and the pre-mixing apparatus, 3-5 is dripped after Precerving liquid splashes into pre-mixing apparatus, close flow regulator
(3). after the venipuncture success, if use the keep sample blood taking bag of bag of band, then unclamp the bag clips that keeps sample earlier, treat that about 15～20 ml blood that are used for blood testing that flow out at first flow into the bag that keeps sample after, folder closes the bag clips that keeps sample again; If use not the blood taking bag with the bag that keeps sample, then unclamp obstruction piece lower end flow-stopping clip, make blood flow directly into pre-mixing apparatus, keep the drop of venipuncture point and blood bag, keep blood flow unobstructed (4). utilize flow regulator dropwise to go into the speed of pre-mixing apparatus according to the kind control Precerving liquid of Precerving liquid, making the blood that enters pre-mixing apparatus and the ratio of Precerving liquid is about 1:7
(5). adopt and mix hemostix or manual blood sampling (should mix 1 time in per at least 90 seconds) continuously, make to flow into the abundant mixing of blood taking bag blood; When the blood volume in the blood taking bag reaches requirement, advise blood donor pine fist, unclamp tourniquet, clamp the needle handle rear tube with mosquito forceps (folder), press the point of puncture top with sterilized cotton ball, dial except syringe needle, press for a moment hemorrhage to not having
(6). after blood sampling finishes, if still leave a small amount of Precerving liquid in Precerving liquid bag and the pipeline, then with blood bag clamp specially Precerving liquid is clamp-oned in the blood taking bag, finish simultaneously keep sample, heat seal, labeling, record and checking work.
(7). utilize multi-joint bag technique, according to different centrifugal rotational speed, time and temperature, under suitable condition, carry out the preparation of blood constituent.
Claims (4)
1. the blood bag system that a minimizing " is gathered damage " is characterized in that having:
The blood bag system that invention is gathered whole blood and carried out the blood constituent preparation according to " in proportion with adding Precerving liquid " principle.
2. blood bag system as claimed in claim 1 is characterized in that its properties of product structure and consists of: blood taking needle, flexible pipe, sealed mouth, intermediate plate, threeway, blood taking bag, flow regulator, pre-mixing apparatus, blood filter, leucocyte-removing filter, the bag that keeps sample, transfering bag, storage blood bag, blood Precerving liquid bag and the alserver's solution bag of rolling.
3. the method for " in proportion with adding Precerving liquid " as claimed in claim 1, it is characterized in that, in whole blood collection procedure, according to the kind of Precerving liquid, add the blood Precerving liquid in proportion, make blood volume in the blood bag and blood Precerving liquid capacity keep correct ratio.
4. blood bag system according to claim 1, set up the blood-sampling method with this system support, it is characterized in that must be in whole blood collection procedure, utilize flow regulator according to the kind of Precerving liquid, the control Precerving liquid is dropwise gone into the speed of pre-mixing apparatus, and making the blood that enters pre-mixing apparatus and the ratio of Precerving liquid is 1:4(ACD-B) or 1:7(CPD and CPDA-1) about; After blood sampling finishes, if still leave a small amount of Precerving liquid in Precerving liquid bag and the pipeline, then with blood bag clamp specially Precerving liquid is clamp-oned in the blood taking bag, made that blood and Precerving liquid remain proper proportion in the blood bag.
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| Application Number | Priority Date | Filing Date | Title |
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| CN2013100531612A CN103203046A (en) | 2013-04-15 | 2013-04-15 | blood bag system for decreasing collection damage and matched blood collection method |
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| Application Number | Priority Date | Filing Date | Title |
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| CN2013100531612A CN103203046A (en) | 2013-04-15 | 2013-04-15 | blood bag system for decreasing collection damage and matched blood collection method |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN114533060A (en) * | 2022-03-01 | 2022-05-27 | 无锡创盟精准医学科技有限公司 | Method for preparing solid or liquid natural concentrated growth factor CGF from autoblood |
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| CN202446542U (en) * | 2012-01-13 | 2012-09-26 | 深圳市血液中心 | Multifunctional blood sampling and transfusing device |
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| US20040186414A1 (en) * | 2003-02-03 | 2004-09-23 | Maurice Behague | Collection bag system with preformed loop |
| WO2004096319A1 (en) * | 2003-04-30 | 2004-11-11 | Crb Nederland B.V. | Method and apparatus for fractionating blood |
| CN102215885A (en) * | 2008-12-03 | 2011-10-12 | 泰尔茂株式会社 | Blood bag system and blood processing method |
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| CN114533060A (en) * | 2022-03-01 | 2022-05-27 | 无锡创盟精准医学科技有限公司 | Method for preparing solid or liquid natural concentrated growth factor CGF from autoblood |
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Application publication date: 20130717 |