CN103202391B - Compound lactobacillus probiotics for effectively preventing and improving bovine mastitis - Google Patents
Compound lactobacillus probiotics for effectively preventing and improving bovine mastitis Download PDFInfo
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Abstract
Disclosed is compound lactobacillus probiotics for effectively preventing and improving bovine mastitis. The compound lactobacillus probiotics is characterized by comprising, by mass, 10% of lactobacillus casei HM-09 (L.casei HM-09), 10% of lactobacillus plantarum HM-10(L.plantarum HM-10) and the rest 80% of zeolite powder, viable count of the L.casei HM-09 in the compound lactobacillus probiotics is more than or equal to 2.5x108cfu/g, and viable count of the L.plantarum HM-10 is more than or equal to 2.5x108cfu/g. The lactobacillus casei and the lactobacillus plantarum are preserved in China General Microbiological Culture Collection Center of China Committee for Culture Collection of Microorganisms, in Datun road, Beijing, on October 29th, 2012, and the preservation numbers of the lactobacillus casei HM-09 (L.casei HM-09) and the lactobacillus plantarum HM-10(L.plantarum HM-10) are CGMCC No.6736 and CGMCC No.6741 respectively.
Description
Technical field
The present invention relates to prevention and improve the compound micro-ecological preparation of garget, especially a kind ofly effectively can reduce somatic number, reduce effective prevention of latent mammitis sickness rate and improve the compound micro-ecological preparation of garget.
Background technology
Mastitis is that in world's milk cow production, sickness rate is the highest, popular the soonest, cause damage one of maximum disease, at Lactation of Dairy Cow phase sickness rate up to more than 78%.Recessive or clinic mastitis all can cause the decline of milk yield and milk quality, causes high risks, comprising: the minimizing directly causing milk yield to milk cow production; Milk processing characteristics is bad, and Quality of Dairy Products is declined; Milk modification; Drug residue makes people not eat; Some milk cows cannot be cured and be eliminated.And the loss that latent mammitis causes is higher than clinic mastitis, this loss is that people can not observe directly.
Utilize antibiotic therapy mastitis to be modal methods for the treatment of, but antibiotic extensive application bring many negative effects, as the appearance etc. of antibiotic remains, Resistant strain.And novel biological control method, herbal treatment, the anti-therapy of probiotics are risen gradually.
Probiotics is utilized to prevent and improve garget, strengthen animal body immunizing power because it has, maintain intestinal microecology balance, produce the several functions such as beneficial metabolic product, and have no side effect, the advantage such as not residual, free from environmental pollution, therefore, can by development probiotics, strengthen immunizing power and the disease resistance of milk cow with probiotics, reach the object of control bovine subclinical mastitis, there is larger potentiality and vast potential for future development.
The present invention is directed to above-mentioned problem, develop a kind of Novel dry Lactobacillus paracasei HM-09 (L.casei HM-09) and plant lactobacillus HM-10(L.plantarum HM-10) compound micro-ecological preparation, definite value is can be good in gastrointestinal tract mucous after feeding cow, pathogenic bacteria is suppressed to grow, improve intestinal beneficial flora, enhancing body immunizing power; Effectively can reduce somatic number, reduce latent mammitis sickness rate.
Summary of the invention
The object of the present invention is to provide and a kind ofly effectively can reduce somatic number, reduce the prevention of latent mammitis sickness rate and improve the compound micro-ecological preparation of garget.
The object of the invention is to be achieved through the following technical solutions:
A kind of effective prevention and improve the compound micro-ecological preparation of garget, it is characterized in that: in every gram of compound micro-ecological preparation, lactobacterium casei HM-09 (L.casei HM-09) mass fraction is 10%, plant lactobacillus HM-10(L.plantarum HM-10) mass fraction is 10%, all the other 80% are zeolite powder, simultaneously L.casei HM-09 number of viable>=2.5 × 10 in compound micro-ecological preparation
8cfu/g, L.plantarum HM-10 number of viable>=2.5 × 10
8cfu/g;
Described lactobacterium casei HM-09 (L.casei HM-09) and plant lactobacillus HM-10(L.plantarum HM-10) be great Tun road, Beijing on October 29th, 2012 in address, the center preservation of China Committee for Culture Collection of Microorganisms's common micro-organisms, lactobacterium casei HM-09 (L.casei HM-09) culture presevation number: CGMCC No.6736; Plant lactobacillus HM-10(L.plantarum HM-10) culture presevation number: CGMCC No.6741.
L.casei HM-09 is separated in healthy Roll road, can be good at definite value in gastrointestinal tract mucous, suppresses pathogenic bacteria to grow, improves intestinal beneficial flora, enhancing body immunizing power; L.plantarum HM-10 is separated in fermented feed, can effective degradative fungi toxin, has good acid resistance, energy for growth is strong, acid production speed is fast.
Described probiotics is in the application prevented and improve in mammitis of cow, and concrete grammar is as follows:
To feed scheme: mixed with feed by 100g probiotics powder, then disposable feeding suffers from the ox of mastitis, feeds continuously 21 days.
To feed probiotics 100 grams, every ox every day each 1 time sooner or later, feed continuously 10 days, then the somatic number in DHI detection method test cow's milk is utilized, result shows: in claim 1 of feeding, compound micro-ecological preparation effectively can improve mammitis of cow, significantly can reduce the somatocyte of suffering from mastitis cow's milk.
Probiotics provided by the invention has following positively effect:
After compound micro-ecological preparation of the present invention mixes with feed, feed and suffer from the milk cow of mastitis, feed every day 100g, feed continuously 21 days, to mastitis ox, there is well prevention and improvement result, mastitis sickness rate can be reduced by 84.7%, sickness rate obviously declines, and improves milk cow health level.
The feed of feeding containing 100 grams of compound micro-ecological preparations, every ox every day each 1 time sooner or later, feed continuously 10 days, effectively can reduce somatic number, reduced rate is 78.3%, improves milk quality.
Accompanying drawing explanation
Accompanying drawing is that mastitis before and after probiotics of feeding improves the schematic diagram of situation.
Embodiment
Below in conjunction with embodiment, the invention will be further described; Following embodiment is illustrative, does not limit the scope of the invention.
Experimental technique in following embodiment, if no special instructions, is ordinary method.
A kind of effective prevention and improve the compound micro-ecological preparation of garget, in every gram of compound micro-ecological preparation, lactobacterium casei HM-09 (L.casei HM-09) mass fraction is 10%, plant lactobacillus HM-10(L.plantarum HM-10) mass fraction is 10%, all the other 80% are zeolite powder, simultaneously L.casei HM-09 number of viable>=2.5 × 10 in compound micro-ecological preparation
8cfu/g, L.plantarum HM-10 number of viable>=2.5 × 10
8cfu/g;
Described lactobacterium casei HM-09 (L.casei HM-09) and plant lactobacillus HM-10(L.plantarum HM-10) be great Tun road, Beijing on October 29th, 2012 in address, the center preservation of China Committee for Culture Collection of Microorganisms's common micro-organisms, lactobacterium casei HM-09 (L.casei HM-09) culture presevation number: CGMCC No.6736; Plant lactobacillus HM-10(L.plantarum HM-10) culture presevation number: CGMCC No.6741.
L.casei HM-09 is separated in healthy Roll road, can be good at definite value in gastrointestinal tract mucous, suppresses pathogenic bacteria to grow, improves intestinal beneficial flora, enhancing body immunizing power; L.plantarum HM-10 is separated in fermented feed, can effective degradative fungi toxin, has good acid resistance, energy for growth is strong, acid production speed is fast.
Experiment 1:L.casei HM-09 and the improvement result of L.plantarum HM-10 compound micro-ecological preparation to the different newborn district of mastitis.
Utilize compound micro-ecological preparation respectively in Heilungkiang, Tianjin, Shandong, the different pasture in Henan and the Inner Mongol test, the 304 Ge Ru districts of 76 mastitis oxen are investigated, feed before probiotics, the detection of CTM method is carried out to each newborn district, diagnose the weight of different newborn districts mastitis degree, the feed of then feeding containing 100 grams of probioticses, every ox every day 1 time, feed continuously 21 days, the detection of CTM method is carried out again to the newborn district of difference, Detection results before and after probiotics of feeding is compared, observes its improvement situation.To feed different newborn districts mastitis changing conditions before and after probiotics in table 1.
Table 1 is fed different newborn district mastitis changing conditions (Zong Ru district 304) before and after probiotics
Note: it is more serious that " ★ " represents mastitis more, efficient=(experiment foremilk district number-test rear milk area number)/experiment foremilk district number) × 100%
From experimental result, this probiotics feed continuously after 21 days, mastitis be improved significantly, and the more serious improved effect in newborn district of mastitis is more obvious, 64.4%, 53.3%, 48.5% is have dropped respectively from high to low by mastitis severity, it can thus be appreciated that this compound micro-ecological preparation has good prevention and improves the effect of garget.
Experiment 2:L.casei HM-09 and L.plantarum HM-10 compound micro-ecological preparation are to improvement result somatic in Bovine Mastitis Milk.
Utilize the different pasture of compound micro-ecological preparation Inner Mongolia, 10 oxen suffering from mastitis carry out somatocyte test, feed before probiotics, utilize the somatic number in DHI detection method test cow's milk, the feed of then feeding containing 100 grams of probioticses, every ox respectively feeds 1 time every day sooner or later, feeds continuously 10 days, then utilize DHI detection method again to test somatic number in cow's milk, the somatic number before and after compound micro-ecological preparation of feeding is compared.Test-results is in table 2.
Table 2 to be fed before and after compound micro-ecological preparation somatic number changing conditions (unit: ten thousand/mL) in cow's milk
Known by table 2 data analysis, in 10 test oxen, obviously declining appears in the somatic number of 9 oxen, only has 1 ox to no effect.If being greater than 500,000/mL by somatic number is clinic mastitis, so test-results illustrates: wherein 4 oxen are cured, 5 ox successfuls.Before the test of 10 oxen, average body cell count are 784.4 ten thousand/mL, and after test, average body cell count is 170.5 ten thousand/mL, have dropped 78.3%, and the compound micro-ecological preparation that shows to feed effectively can improve mammitis of cow, reduces the somatocyte in cow's milk.
Experiment 3:L.casei HM-09 and L.plantarum HM-10 compound micro-ecological preparation are to the improvement result of mammitis of cow breast.
85 oxen of compound micro-ecological preparation to the different pasture in Heilungkiang, Tianjin, Shandong, Henan and the Inner Mongol are utilized to test, consider from improving mastitis whole structure: namely from improving at least one newborn district breast, or more serious newborn district has clear improvement and sets out, investigate probiotics to the improvement situation of mastitis; The feed that process of the test is fed containing 100 grams of compound micro-ecological preparations to every ox, every ox every day 1 time, feed continuously 21 days, by the improvement situation of mastitis before and after CTM method determination test, result shows that 72 cow head mastitises are obviously improved, efficiently reach 84.7%, test-results See Figure 1, illustrate that this compound micro-ecological preparation can effectively prevent and improve mammitis of cow.
Claims (1)
1. the compound micro-ecological preparation effectively preventing and improve garget, it is characterized in that: in every gram of compound micro-ecological preparation, lactobacterium casei HM-09 (L.casei HM-09) mass fraction is 10%, plant lactobacillus HM-10 (L.plantarum HM-10) mass fraction is 10%, all the other 80% are zeolite powder, simultaneously L.casei HM-09 number of viable>=2.5 × 10 in compound micro-ecological preparation
8cfu/g, L.plantarum HM-10 number of viable>=2.5 × 10
8cfu/g;
Described L.casei HM-09 is separated in healthy Roll road, has excellent acidproof, bile tolerance and enteron aisle definite value ability, can promote intestinal mucosal immune, suppress enteropahtogenic microganism, regulating intestinal canal flora; Described L.plantarum HM-10 is separated in fermented feed, can effective degradative fungi toxin, suppression enteropahtogenic microganism; Above-mentioned two strain bacterial strains have been preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center, lactobacterium casei HM-09 (L.casei HM-09) culture presevation number: CGMCC No.6736; Plant lactobacillus HM-10 (L.plantarum HM-10) culture presevation number: CGMCC No.6741.
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| CN105517557B (en) | 2013-04-23 | 2021-11-30 | 特雷根控股有限公司 | Bacterial strains having antimicrobial activity and biocontrol compositions comprising said strains |
| CN104510779B (en) * | 2013-09-29 | 2017-12-22 | 瑞普（天津）生物药业有限公司 | A kind of Chinese medicine microecological preparation for treating cow subclinical mastitis |
| CN103952336B (en) * | 2014-03-28 | 2016-06-29 | 内蒙古和美科盛生物技术有限公司 | Bacillus licheniformis, bacillus subtilis and lactobacillus casei preparation and preparation |
| CN103911328B (en) * | 2014-03-28 | 2017-01-18 | 内蒙古和美科盛生物技术有限公司 | Preparation method of bacillus coagulans and lactobacillus casei compound bacterial preparation |
| CN103911324B (en) * | 2014-03-28 | 2016-05-25 | 内蒙古和美科盛生物技术有限公司 | Contain probiotics preparation of Lactobacillus plantarum and bacillus licheniformis and preparation method thereof |
| CN103981123B (en) * | 2014-03-28 | 2016-06-15 | 内蒙古和美科盛生物技术有限公司 | Probiotics and preparation method thereof containing Lactobacillus plantarum, Bifidobacterium lactis |
| CN103911323B (en) * | 2014-03-28 | 2016-06-29 | 内蒙古和美科盛生物技术有限公司 | Bacillus licheniformis, bacillus subtilis and lactobacillus plantarum preparation and preparation |
| CN103911325B (en) * | 2014-03-28 | 2016-04-27 | 内蒙古和美科盛生物技术有限公司 | Bacillus coagulans, lactobacterium casei, plant lactobacillus probiotic agent and preparation |
| CN103952345B (en) * | 2014-04-17 | 2017-01-11 | 青岛九和宜生生物科技有限公司 | Probiotic preparation containing Lactobacillus casei and Bacillus licheniformis and preparation method thereof |
| CN103952346B (en) * | 2014-04-17 | 2016-08-31 | 青岛九和宜生生物科技有限公司 | Lactobacillus plantarum, lactobacillus casei and the preparation of Bacillus licheniformis composite bacteria agent capable |
| CN103937715B (en) * | 2014-04-17 | 2017-03-29 | 青岛九和宜生生物科技有限公司 | Tiny ecosystem probiotics preparation containing lactobacillus casei and preparation method thereof |
| CN104017751B (en) * | 2014-04-17 | 2017-03-29 | 青岛九和宜生生物科技有限公司 | Bacillus coagulans, Lactobacillus plantarum and Lactobacillus casei preparation and its preparation |
| CN104208118B (en) * | 2014-09-11 | 2018-03-06 | 保定冀中药业有限公司 | Reduce the Chinese medicine and probiotics combination and preparation method of milk cow somatic number |
| CN105748534B (en) * | 2016-04-29 | 2018-12-18 | 内蒙古和美科盛生物技术有限公司 | A kind of compound lactobacillus nipple cleaning solution improving mammilla of milk cattle microecosystem |
| CN109306327A (en) * | 2017-07-27 | 2019-02-05 | 内蒙古蒙正牧康实业有限公司 | The fresh storage fermenting agent of niblet and fresh storage zymotechnique |
| CN117070413A (en) * | 2023-08-21 | 2023-11-17 | 广东海洋大学 | Lactobacillus paracasei BY5 and application thereof |
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