CN103202246B - The indoor cultural method of a kind of Japan large chela roach - Google Patents
The indoor cultural method of a kind of Japan large chela roach Download PDFInfo
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- CN103202246B CN103202246B CN201210016014.3A CN201210016014A CN103202246B CN 103202246 B CN103202246 B CN 103202246B CN 201210016014 A CN201210016014 A CN 201210016014A CN 103202246 B CN103202246 B CN 103202246B
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- roach
- japan
- sediments
- chela
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
Abstract
The present invention discloses the cultural method of the large chela roach of the biological Japan of a kind of amphipoda.Concrete steps are as follows: the large chela roach individuality of field acquisition Japan and sampling site seawater; After cleaning culture vessel, add clean sediments in a reservoir, cultivate seawater; Select the large chela roach of Japan individual, be placed in ready culture vessel, every day, liter (falling) temperature was no more than 3 DEG C, rise (falling) salinity and be no more than 3, domestication is to laboratory long term culture conditions gradually: temperature 20 ~ 26 DEG C, salinity 10 ~ 26, Micro Algae bait of throwing something and feeding, additional synthetic diet; Water is changed, bait of throwing something and feeding after changing water every one day; Oxygenation; 30-50d changes sediments, is separated adult and the young.The present invention cultivates the large chela roach of the Japan obtained, and the living bait that can be used as the economic livings such as fish, shrimp, crab is biological, also can be used as the biological subject of marine sediment, ocean dredging thing and seawater sample toxicity detection, has broad application prospects.
Description
Technical field
The present invention relates to marine benthos to cultivate, be specially the indoor cultural method of the large chela roach of a kind of amphipoda Japan.
Background technology
Amphipoda is the important component part of marine benthos, is the main source of fish and most of birds food; Kind is many, distribution is wide, has all over the world; Quantity is large, the breeding cycle is short.Amphipoda because of above-mentioned advantage become bio-toxicity detect preferred biological subject.
But mostly come from field acquisition for the amphipoda biological subject of toxicity detection, thus there is the problem of the aspects such as biological subject acquisition time, quantity, biological quality, cause toxicity detection work to be extremely restricted.For meeting toxicity detection requirements of one's work, the laboratory anniversary long-term cultivation of amphipoda biological subject is very necessary.
Current amphipoda biological subject can accomplish the kind that the kind of long-term cultivation in the lab only has Ampelisca abdita, Leptocheirus plumulosus, Hyalella azteca etc. few, during the laboratory cultures technology comprising the kinds such as Corophium volutator is constantly groped.
The large chela roach (Grandidierella japonica) of Japan is under the jurisdiction of the large chela roach of Amphipoda Gammaridea Corophiidae and belongs to, and inhabits intertidal zone ooze and silt substrate.Be found in Japan at first, for the world extensively divides fabric, all there is report the Atlantic Ocean and pacific rim, and all have distribution at China's Bohai and Yellow Seas and the East Sea, the anniversary occurs.This kind of biology by U.S. material with test the standard biological subject learning to detect as sediment toxicity.The present invention is specially the seawater acclimation of the large chela roach of a kind of amphipoda Japan and indoor cultural method, cultivate the biological subject that the large chela roach of Japan obtained can be used as the diagnosis of environmental pollution ecotoxicological, ensure the anniversary supply of the large chela roach of Japan, meet marine sediment, the carrying out of ocean dredging thing and the testing of seawater sample bio-toxicity, solve environmental pollution biological detection biological subject Problems existing in acquisition time, quantity, biological quality etc.
Summary of the invention
The present invention is the problem in order to solve existing for prior art, and object is the cultural method providing the large chela roach of the biological Japan of a kind of amphipoda, provides quantity abundance and colory biological subject, meets toxicity detection requirements of one's work.
The present invention is domestication and cultivates the large chela roach of Japan, make its through domestication after under the optimal culture condition of laboratory Growth and reproduction, reach this kind of biology can in laboratory long-term cultivation, ensure the large chela roach laboratory anniversary supply of Japan, meet the requirement of toxicity detection work to biological subject acquisition time, quantity and biological quality, ensure carrying out smoothly of toxicity detection work.
For achieving the above object, the technical solution used in the present invention is as follows:
An aspect of of the present present invention is: the indoor cultural method of the large chela roach of a kind of amphipoda Japan, it comprises the steps:
A. the large chela roach of Japan gathers
Gather the surface deposit of large chela roach habitat about the 5cm of Japan, put into the sieve that mesh is 0.5mm, sift out sediments, collect amphipoda, put it in the container filling on-the-spot seawater, lucifuge oxygenation in transportation;
B. the large chela roach culture matrix of Japan
The clean sediments of culture vessel splendid attire 2-3cm, adds 3 times that seawater bulk is volume of sediment;
C. the large chela roach acclimation conditions of Japan
Select the large chela roach of Japan individual, be placed in the culture vessel got ready, Micro Algae bait of throwing something and feeding, oxygenation; Water is changed, Micro Algae bait of throwing something and feeding after changing water, oxygenation every one day; Every day rises or the temperature of falling is no more than 3 DEG C, rises or the salinity of falling is no more than 3, and domestication is to laboratory long term culture conditions gradually;
D. the large chela roach condition of culture of Japan
Temperature 20 ~ 26 DEG C, salinity 10 ~ 26, Micro Algae bait of throwing something and feeding, oxygenation; Water is changed, Micro Algae bait of throwing something and feeding after changing water every one day; 30 ~ 50d changes sediments, is separated adult and the young.
Concrete, in above-mentioned cultural method, the culture vessel described in step b, after clean, be that 10%HCl solution at least soaks 6 hours by concentration, rinse well with water.
Concrete, in above-mentioned cultural method, described in step b:
Cultivate sediments: gather from the large chela roach of Japan locality, the sieve through 0.5mm sieves, and remove stone and bulky grain thing, the sediments after sieving holds in a reservoir, is placed in the frozen at least 24h of-20 DEG C, refrigerator, removes the other biological in sediments; Frozen sediments is put into 60 DEG C, baking oven to dry or natural air drying, pulverize rear for subsequent use;
Cultivate seawater: its salinity is that coastal seawater is allocated gained through sand filtration, precipitation and 20 μm of silk cover filterings with the running water after aeration.
Concrete, in above-mentioned cultural method, the volume changing water described in step c or d is 2/3 of former interpolation seawater bulk.
Concrete, in above-mentioned cultural method, the Micro Algae bait described in step c or d is following one:
6 × 10
6the Nitzschia closterium minutissima of cell/ml;
6 × 10
5the Platymonas helgolandica var of cell/ml;
Nitzschia closterium minutissima (6 × 10
6and Platymonas helgolandica var (6 × 10 cell/ml)
5cell/ml) with equal-volume mixed solution;
Nitzschia closterium minutissima (6 × 10
6and Platymonas helgolandica var (6 × 10 cell/ml)
5cell/ml) with equal-volume mixed solution, additional synthetic diet (0.1mg/ml); Described synthetic diet be the prawn zoea stage or acanthosoma synthetic diet used.
The present invention is the large chela roach of indoor cultivation Japan, make it can Growth and reproduction under laboratory cultures condition after domestication, reach this kind of biology can in laboratory long-term cultivation, for marine fish, shrimp, crab provide high-quality living bait, for toxicity detection work provides quantity and the colory biological subject of biology.
Embodiment
Following non-limiting example can make the present invention of those of ordinary skill in the art's comprehend, but does not limit the present invention in any way.
Embodiment 1
A. the large chela roach of Japan gathers
In morning or afternoon low tide one, select the large chela roach population density higher position of Japan, with spade about scraping 5cm surface deposit gently, put into the stainless steel sieve that mesh is 0.5mm, sift out sediments, collect amphipoda, put it in the Plastic Drum filling on-the-spot seawater, avoid illumination, take back laboratory rapidly; As needed long-distance transport, need oxygenation, to ensure that in seawater, oxygen is sufficient.
B. the large chela roach culture matrix of Japan
In laboratory, select the square plastic tank of 30 × 20 × 15cm; After cleaning culture vessel, be that 10%HCl solution soaks at least 6 hours by concentration, then clean with fresh water flush.Add clean sediments, clean seawater in a reservoir, for subsequent use;
Cultivate sediments: for gathering from the large chela roach of Japan locality, the sieve through 0.5mm sieves, and remove stone and bulky grain thing, the sediments after sieving holds in a reservoir, is placed in the frozen at least 24h of-20 DEG C, refrigerator, removes the other biological in sediments.Frozen sediments is put into 60 DEG C, baking oven to dry or natural air drying, for biological culture after pulverizing.
Cultivate seawater: its salinity is that coastal seawater is allocated gained through sand filtration, precipitation and 20 μm of silk cover filterings with the running water after aeration.Sediments cultivated by culture vessel splendid attire is that 2-3cm is thick, adds 3 times that seawater bulk is volume of sediment.
C. the large chela roach acclimation conditions of Japan
Select the large chela roach of Japan individual, be placed in the culture vessel got ready, bait of throwing something and feeding, oxygenation; Changed water every one day, the volume changing water is every other day 2/3 of former interpolation seawater bulk.To throw something and feed after changing water bait, oxygenation.Every day, liter (falling) temperature was no more than 3 DEG C, and rise (falling) salinity and be no more than 3, domestication is to laboratory long term culture conditions gradually.
Described bait is: Nitzschia closterium minutissima (6 × 10
6and Platymonas helgolandica var (6 × 10 cell/ml)
5cell/ml) 1: 1 solution formed, additional synthetic diet (0.1mg/ml).
D. the large chela roach condition of culture of Japan
Laboratory long term culture conditions is: temperature 20 ± 1 DEG C, salinity 25 ± 1, bait of throwing something and feeding, oxygenation; Water is changed, bait of throwing something and feeding after changing water every one day; 30 ~ 50d changes sediments, is separated adult and the young.
Described bait is: Nitzschia closterium minutissima (6 × 10
6and Platymonas helgolandica var (6 × 10 cell/ml)
5cell/ml) 1: 1 solution formed, additional synthetic diet (0.1mg/ml).Described synthetic diet be the prawn zoea stage or acanthosoma synthetic diet used.
Embodiment 2
Basic cultural method is with embodiment 1, and difference is:
The culture vessel of described step b is 20-25L round plastic basin.
In described step c or d, bait of throwing something and feeding is: 6 × 10
6the Nitzschia closterium minutissima of cell/ml.
Embodiment 3
Basic cultural method is with embodiment 1, and difference is:
The culture vessel of described step b is 20-25L round plastic basin.
In described step c or d, bait of throwing something and feeding is: 6 × 10
5the Platymonas helgolandica var of cell/ml.
Embodiment 4
Basic cultural method is with embodiment 1, and difference is:
The culture vessel of described step b is 20-25L round plastic groove.
In described step c or d, bait of throwing something and feeding is: Platymonas helgolandica var (6 × 10
5and Nitzschia closterium minutissima (6 × 10 cell/ml)
6cell/ml) the two isopyknic mixed liquor.
Embodiment 5
Basic cultural method is with embodiment 1, and difference is:
The culture vessel of described step b is 20-25L round plastic basin.
Described cultivation temperature can be 20 ~ 26 DEG C, accelerated the Growth and reproduction of the large chela roach of Japan, but temperature raising every day is no more than 3 DEG C by raised temperature.
Claims (1)
1. an indoor cultural method for the large chela roach of amphipoda Japan, is characterized in that, comprise the steps:
A. the large chela roach of Japan gathers
Gather the surface deposit of large chela roach habitat about the 5cm of Japan, put into the sieve that mesh is 0.5mm, sift out sediments, collect amphipoda, put it in the container filling on-the-spot seawater, lucifuge oxygenation in transportation;
B. the large chela roach culture matrix of Japan
The clean sediments of culture vessel splendid attire 2-3cm, adds 3 times that seawater bulk is volume of sediment;
C. the large chela roach acclimation conditions of Japan
Select the large chela roach of Japan individual, be placed in the culture vessel got ready, Micro Algae bait of throwing something and feeding, oxygenation; Water is changed, Micro Algae bait of throwing something and feeding after changing water, oxygenation every one day; Every day rises or the temperature of falling is no more than 3 DEG C, rises or the salinity of falling is no more than 3, and domestication is to laboratory long term culture conditions gradually;
D. the large chela roach condition of culture of Japan
Temperature 20 ~ 26 DEG C, salinity 10 ~ 26, Micro Algae bait of throwing something and feeding, oxygenation; Water is changed, Micro Algae bait of throwing something and feeding after changing water every one day; 30 ~ 50d changes sediments, is separated adult and the young;
Culture vessel described in step b, after clean, be that 10%HCl solution at least soaks 6 hours by concentration, rinse well with water;
Described in step b:
Cultivate sediments: gather from the large chela roach of Japan locality, the sieve through 0.5mm sieves, and remove stone and bulky grain thing, the sediments after sieving holds in a reservoir, is placed in the frozen at least 24h of-20 DEG C, refrigerator, removes the other biological in sediments; Frozen sediments is put into 60 DEG C, baking oven to dry or natural air drying, pulverize rear for subsequent use;
Cultivate seawater: its salinity is that coastal seawater is allocated gained through sand filtration, precipitation and 20 μm of silk cover filterings with the running water after aeration;
The volume changing water described in step c or d is 2/3 of former interpolation seawater bulk;
Micro Algae bait described in step c or d is following one:
6 × 10
6the Nitzschia closterium minutissima of cell/ml;
6 × 10
5the Platymonas helgolandica var of cell/ml;
6 × 10
6the Nitzschia closterium minutissima of cell/ml and 6 × 10
5the Platymonas helgolandica var equal-volume mixed solution of cell/ml;
6 × 10
6the Nitzschia closterium minutissima of cell/ml and 6 × 10
5the Platymonas helgolandica var equal-volume mixed solution of cell/ml, the synthetic diet of additional 0.1mg/ml; Described synthetic diet be the prawn zoea stage or acanthosoma synthetic diet used.
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|---|---|---|---|
| CN201210016014.3A CN103202246B (en) | 2012-01-17 | 2012-01-17 | The indoor cultural method of a kind of Japan large chela roach |
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|---|---|---|---|
| CN201210016014.3A CN103202246B (en) | 2012-01-17 | 2012-01-17 | The indoor cultural method of a kind of Japan large chela roach |
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| CN103202246A CN103202246A (en) | 2013-07-17 |
| CN103202246B true CN103202246B (en) | 2015-08-26 |
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Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2011244795A (en) * | 2010-05-21 | 2011-12-08 | Minamikyushu City | Method for producing on land of sand-submerged bivalves, such as littleneck clam |
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| CN101560113B (en) * | 2008-04-19 | 2014-01-08 | 黄定忠 | Hard surface treatment composition and application thereof, cladding formed after hard surface treatment and hard surface material with same |
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Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2011244795A (en) * | 2010-05-21 | 2011-12-08 | Minamikyushu City | Method for producing on land of sand-submerged bivalves, such as littleneck clam |
Non-Patent Citations (4)
| Title |
|---|
| 实验室培养日本大螯蜚f1代的生活史;王睿睿等;《海洋环境科学》;20090620;第28卷(第3期);第272-274、287页 * |
| 实验室培养的端足类日本大螯蜚F1代对Cd的急性毒性响应;闫启仑等;《海洋环境科学》;20070620;第26卷(第3期);第225-228页 * |
| 室内培养底栖端足类日本大螯蜚饵料研究;陈红星等;《海洋环境科学》;19980220;第17卷(第1期);第21-25页 * |
| 盐度对端足类日本大螯蜚生长发育的影响;韩明辅等;《海洋科学环境》;19980520;第17卷(第2期);第40-44页 * |
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