CN103202245B - The indoor cultural method of a kind of river Guo Luo roach - Google Patents
The indoor cultural method of a kind of river Guo Luo roach Download PDFInfo
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- CN103202245B CN103202245B CN201210014486.5A CN201210014486A CN103202245B CN 103202245 B CN103202245 B CN 103202245B CN 201210014486 A CN201210014486 A CN 201210014486A CN 103202245 B CN103202245 B CN 103202245B
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- river
- sediments
- guo luo
- roach
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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Abstract
The present invention discloses the cultural method of a kind of amphipoda river Guo Luo roach.Concrete steps are as follows: field acquisition river Guo Luo roach individuality and sampling site seawater; After cleaning culture vessel, add clean sediments, clean seawater in a reservoir; Select river Guo Luo roach individual, be placed in ready culture vessel, every day, liter (falling) temperature was no more than 3 DEG C, rise (falling) salinity and be no more than 3, domestication is to laboratory long term culture conditions gradually: temperature 15 ~ 25 DEG C, salinity 22 ~ 26, Micro Algae bait of throwing something and feeding, additional synthetic diet; Water is changed, bait of throwing something and feeding after changing water every one day; Oxygenation; 30-50d changes sediments, is separated adult and the young.The present invention cultivates the river Guo Luo roach obtained, and the living bait that can be used as the economic livings such as fish, shrimp, crab is biological, also can be used as the biological subject of marine sediment, ocean dredging thing and seawater sample toxicity detection, has broad application prospects.
Description
Technical field
The present invention relates to marine benthos to cultivate, be specially the indoor cultural method of a kind of amphipoda river Guo Luo roach.
Background technology
Amphipoda Gammaridea Guo Luo roach belongs to for universal large genus, and being benthic important component part, is the high-quality bait of marine fish, shrimp, crab class.First Guo Luo roach is applied to cultured fishes, and due to the development of shrimp culture industry, research finds that Guo Luo roach is also that prawn is young, the high-quality living bait of juvenile prawn life stage.
Guo Luo roach belongs to biological or the preferred biological subject of toxicity detection, Partial Species has been applied to bio-toxicity and has detected, research wherein for Corophium valutator is comparatively comprehensive, and Environment Canada it can be used as the standard biological subject of sediment toxicity biological detection.Corophium orientale, Corophium colo, Corophium multisetosum and Corophiuminsidiosum are also underway as the research of biological subject.
River Guo Luo roach (corophium acherusicum) belongs to one of 20 kinds of biologies found for China sea Guo Luo roach, inhabits intertidal zone ooze and silt substrate, all has distribution in countries such as China, Japan, Australia, New Zealand.No matter as food organisms or the biological subject of toxicity detection, the research for this kind of biology laboratory cultural method is all very necessary.The present invention is specially the indoor cultural method of a kind of amphipoda river Guo Luo roach, cultivates the river Guo Luo roach obtained and can be used as high-quality bait, for economic living cultivation such as marine fish, shrimp, crab classes, improves the output of economic living; Cultivate the biological subject that the river Guo Luo roach obtained also can be used as the diagnosis of environmental pollution ecotoxicological, ensure the anniversary supply of river Guo Luo roach, meet marine sediment, the carrying out of ocean dredging thing and the testing of seawater sample bio-toxicity, solve environmental pollution biological detection biological subject Problems existing in acquisition time, quantity, biological quality etc.
Summary of the invention
The present invention is the problem in order to solve existing for prior art, object is the indoor cultural method providing a kind of amphipoda river Guo Luo roach, for marine fish, shrimp, crab class etc. provide high-quality bait, for toxicity detection work provides quantity abundance and colory biological subject.
For achieving the above object, the technical solution used in the present invention is as follows:
An aspect of of the present present invention is: the indoor cultural method of a kind of amphipoda river Guo Luo roach, it comprises the steps:
A. river Guo Luo roach gathers
Gather the surface deposit of river Guo Luo roach habitat about 5cm, put into the sieve that mesh is 0.5mm, sift out sediments, collect amphipoda, put it in the container filling on-the-spot seawater, lucifuge oxygenation in transportation;
B. river Guo Luo roach culture matrix
The clean sediments of culture vessel splendid attire 2-3cm, adds 3 times that seawater bulk is volume of sediment;
C. river Guo Luo roach acclimation conditions
Select river Guo Luo roach individual, be placed in the culture vessel got ready, Micro Algae bait of throwing something and feeding, oxygenation; Water is changed, Micro Algae bait of throwing something and feeding after changing water, oxygenation every one day; Every day rises or the temperature of falling is no more than 3 DEG C, rises or the salinity of falling is no more than 3, and domestication is to laboratory long term culture conditions gradually;
D. river Guo Luo roach condition of culture
Temperature 15 ~ 25 DEG C, salinity 22 ~ 26, Micro Algae bait of throwing something and feeding, oxygenation; Water is changed, Micro Algae bait of throwing something and feeding after changing water every one day; 30 ~ 50d changes sediments, is separated adult and the young.
Concrete, in above-mentioned cultural method, the culture vessel described in step b, after clean, be that 10%HCl solution at least soaks 6 hours by concentration, rinse well with water.
Concrete, in above-mentioned cultural method, described in step b:
Cultivate sediments: gather from river Guo Luo roach locality, the sieve through 0.5mm sieves, remove stone and bulky grain thing, the sediments after sieving holds in a reservoir, is placed in the frozen at least 24h of-20 DEG C, refrigerator, removes the other biological in sediments; Frozen sediments is put into 60 DEG C, baking oven to dry or natural air drying, pulverize rear for subsequent use;
Cultivate seawater: its salinity is that coastal seawater is allocated gained through sand filtration, precipitation and 20 μm of silk cover filterings with the running water after aeration.
Concrete, in above-mentioned cultural method, the volume changing water described in step c or d is 2/3 of former interpolation seawater bulk.
Concrete, in above-mentioned cultural method, the Micro Algae bait described in step c or d is following one:
6 × 10
6the Nitzschia closterium minutissima of cell/ml;
6 × 10
5the Platymonas helgolandica var of cell/ml;
Nitzschia closterium minutissima (6 × 10
6and Platymonas helgolandica var (6 × 10 cell/ml)
5cell/ml) with equal-volume mixed solution;
Nitzschia closterium minutissima (6 × 10
6and Platymonas helgolandica var (6 × 10 cell/ml)
5cell/ml) with equal-volume mixed solution, additional synthetic diet (0.1mg/ml); Described synthetic diet be the prawn zoea stage or acanthosoma synthetic diet used.
The present invention is indoor cultivation river Guo Luo roach, make it can Growth and reproduction under laboratory cultures condition after domestication, reach this kind of biology can in laboratory long-term cultivation, for marine fish, shrimp, crab provide high-quality living bait, for toxicity detection work provides quantity abundance and the colory biological subject of biology.
Embodiment
Following non-limiting example can make the present invention of those of ordinary skill in the art's comprehend, but does not limit the present invention in any way.
Embodiment 1
A. river Guo Luo roach gathers
In morning or afternoon low tide one, select river Guo Luo roach population density higher position, with spade about scraping 5cm surface deposit gently, put into the stainless steel sieve that mesh is 0.5mm, sift out sediments, collect amphipoda, put it in the Plastic Drum filling on-the-spot seawater, avoid illumination, take back laboratory rapidly; As needed long-distance transport, need oxygenation, to ensure that in seawater, oxygen is sufficient.
B. river Guo Luo roach culture matrix
In laboratory, select the square plastic tank of 30 × 20 × 15cm; After cleaning culture vessel, be that 10%HCl solution soaks at least 6 hours by concentration, then clean with fresh water flush.Add clean sediments, clean seawater in a reservoir, for subsequent use;
Cultivate sediments: for gathering from river Guo Luo roach locality, the sieve through 0.5mm sieves, and remove stone and bulky grain thing, the sediments after sieving holds in a reservoir, is placed in the frozen at least 24h of-20 DEG C, refrigerator, removes the other biological in sediments.Frozen sediments is put into 60 DEG C, baking oven to dry or natural air drying, for biological culture after pulverizing.
Cultivate seawater: its salinity is that coastal seawater is allocated gained through sand filtration, precipitation and 20 μm of silk cover filterings with the running water after aeration.The clean sediments of culture vessel splendid attire is that 2-3cm is thick, adds 3 times that seawater bulk is volume of sediment.
C. river Guo Luo roach acclimation conditions
Select river Guo Luo roach individual, be placed in the culture vessel got ready, bait of throwing something and feeding, oxygenation; Changed water every one day, the volume changing water is every other day 2/3 of former interpolation seawater bulk.To throw something and feed after changing water bait, oxygenation.Every day, liter (falling) temperature was no more than 3 DEG C, and rise (falling) salinity and be no more than 3, domestication is to laboratory long term culture conditions gradually.
Described bait is: Nitzschia closterium minutissima (6 × 10
6and Platymonas helgolandica var (6 × 10 cell/ml)
5cell/ml) 1: 1 solution formed, additional synthetic diet (0.1mg/ml).
D. river Guo Luo roach condition of culture
Laboratory long term culture conditions is: temperature 15 ~ 25 DEG C, salinity 22 ~ 26, bait of throwing something and feeding, oxygenation; Water is changed, bait of throwing something and feeding after changing water every one day; 30 ~ 50d changes sediments, is separated adult and the young.
Described bait is: Nitzschia closterium minutissima (6 × 10
6and Platymonas helgolandica var (6 × 10 cell/ml)
5cell/ml) 1: 1 solution formed, additional synthetic diet (0.1mg/ml).Described synthetic diet be the prawn zoea stage or acanthosoma synthetic diet used.
Embodiment 2
Basic cultural method is with embodiment 1, and difference is:
The culture vessel of described step b is 20-25L round plastic basin.
In described step c or d, bait of throwing something and feeding is: 6 × 10
6the Nitzschia closterium minutissima of cell/ml.
Embodiment 3
Basic cultural method is with embodiment 1, and difference is:
The culture vessel of described step b is 20-25L round plastic basin.
In described step c or d, bait of throwing something and feeding is: 6 × 10
5the Platymonas helgolandica var of cell/ml.
Embodiment 4
Basic cultural method is with embodiment 1, and difference is:
The culture vessel of described step b is 20-25L round plastic basin.
In described step c or d, bait of throwing something and feeding is: Platymonas helgolandica var (6 × 10
5and Nitzschia closterium minutissima (6 × 10 cell/ml)
6cell/ml) the two isopyknic mixed liquor.
Claims (1)
1. an indoor cultural method for amphipoda river Guo Luo roach, is characterized in that, comprise the steps:
A. river Guo Luo roach gathers
Gather the surface deposit of river Guo Luo roach habitat about 5cm, put into the sieve that mesh is 0.5mm, sift out sediments, collect amphipoda, put it in the container filling on-the-spot seawater, lucifuge oxygenation in transportation;
B. river Guo Luo roach culture matrix
The clean sediments of culture vessel splendid attire 2-3cm, adds 3 times that seawater bulk is volume of sediment;
C. river Guo Luo roach acclimation conditions
Select river Guo Luo roach individual, be placed in the culture vessel got ready, Micro Algae bait of throwing something and feeding, oxygenation; Water is changed, Micro Algae bait of throwing something and feeding after changing water, oxygenation every one day; Every day rises or the temperature of falling is no more than 3 DEG C, rises or the salinity of falling is no more than 3, and domestication is to laboratory long term culture conditions gradually;
D. river Guo Luo roach condition of culture
Temperature 15 ~ 25 DEG C, salinity 22 ~ 26, Micro Algae bait of throwing something and feeding, oxygenation; Water is changed, Micro Algae bait of throwing something and feeding after changing water every one day; 30 ~ 50d changes sediments, is separated adult and the young;
Culture vessel described in step b, after clean, be that 10%HCl solution at least soaks 6 hours by concentration, rinse well with water;
Described in step b:
Cultivate sediments: gather from river Guo Luo roach locality, the sieve through 0.5mm sieves, remove stone and bulky grain thing, the sediments after sieving holds in a reservoir, is placed in the frozen at least 24h of-20 DEG C, refrigerator, removes the other biological in sediments; Frozen sediments is put into 60 DEG C, baking oven to dry or natural air drying, pulverize rear for subsequent use;
Cultivate seawater: its salinity is that coastal seawater is allocated gained through sand filtration, precipitation and 20 μm of silk cover filterings with the running water after aeration;
The volume changing water described in step c or d is 2/3 of former interpolation seawater bulk;
Micro Algae bait described in step c or d is following one:
6 × 10
6the Nitzschia closterium minutissima of cell/ml;
6 × 10
5the Platymonas helgolandica var of cell/ml;
6 × 10
6the Nitzschia closterium minutissima of cell/ml and 6 × 10
5the Platymonas helgolandica var of cell/ml is with equal-volume mixed solution;
6 × 10
6the Nitzschia closterium minutissima of cell/ml and 6 × 10
5the Platymonas helgolandica var of cell/ml, with equal-volume mixed solution, adds the synthetic diet of 0.1mg/ml; Described synthetic diet be the prawn zoea stage or acanthosoma synthetic diet used.
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| CN103202245B true CN103202245B (en) | 2015-08-26 |
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| CN103454381A (en) * | 2013-09-10 | 2013-12-18 | 沈阳航空航天大学 | Biological diagnosis method of sediment toxicity |
| CN107691318B (en) * | 2017-11-13 | 2020-04-17 | 烟台大学 | Comprehensive ecological corophium acherusicum cultivation method |
| CN112293308A (en) * | 2020-11-19 | 2021-02-02 | 广东大麟洋海洋生物有限公司 | Golden pomfret breeding method |
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| US20010045189A1 (en) * | 1999-12-30 | 2001-11-29 | Mcneil Roderick J. | Shrimp culture system |
| JP2011244795A (en) * | 2010-05-21 | 2011-12-08 | Minamikyushu City | Method for producing on land of sand-submerged bivalves, such as littleneck clam |
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Non-Patent Citations (3)
| Title |
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| 室内培养底栖端足类日本大螯蜚饵料研究;陈红星等;《海洋环境科学》;19980220;第17卷(第1期);第21-25页 * |
| 河蜾蠃蜚对镉的急性毒性响应;王睿睿等;《生态毒理学报》;20100215;第5卷(第1期);第87-90页 * |
| 王超等.端足类河蜾蠃蜚生活周期及其沉积物毒理敏感性研究.《华东师范大学学报(自然科学版)》.2009,(第3期),第1-6、16页. * |
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