CN103202245A - Indoor cultivation method of corophium acherusicum - Google Patents

Indoor cultivation method of corophium acherusicum Download PDF

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Publication number
CN103202245A
CN103202245A CN2012100144865A CN201210014486A CN103202245A CN 103202245 A CN103202245 A CN 103202245A CN 2012100144865 A CN2012100144865 A CN 2012100144865A CN 201210014486 A CN201210014486 A CN 201210014486A CN 103202245 A CN103202245 A CN 103202245A
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sediments
river
bait
seawater
water
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CN103202245B (en
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王睿睿
闫启仑
关道明
梁军辉
林志浩
王超
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National Marine Environmental Monitoring Center
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National Marine Environmental Monitoring Center
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    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
    • Y02A40/81—Aquaculture, e.g. of fish

Abstract

The invention discloses a cultivation method of amphipoda corophium acherusicum. The cultivation method comprises the following concrete steps that corophium acherusicum individuals are collected in the field, and local seawater is collected; after a cultivation container is cleaned, clean sediments and clean seawater are added into the container; the corophium acherusicum individuals are selected and are placed into the prepared cultivation container, the rise (drop) temperature in each day does not exceed 3 DEG C, the rise (drop) salinity does not exceed 3, and the corophium acherusicum individuals are gradually domesticated to the laboratory long-period cultivation conditions that the temperature is 15 to 25 DEG C, the salinity is 22 to 26, chlamydomonas bait is fed, and synthesized bait is added; water is replaced every two days, and the bait is fed after the water is replaced; the oxygenation is carried out; sediments are replaced after 30 to 50 days, and adult bodies and young bodies are separated. The corophium acherusicum obtained through the cultivation by the cultivation method can be used as live bait organisms of economic organisms such as fishes, shrimps and crabs and can also be used as testing organisms for detecting the toxicity of ocean sediments, ocean dredged materials and seawater samples, and wide application prospects are realized.

Description

The indoor cultural method of a kind of river Guo Luo roach
Technical field
The present invention relates to marine benthos and cultivate, be specially the indoor cultural method of a kind of amphipoda river Guo Luo roach.
Background technology
Amphipoda Gammaridea Guo Luo roach belongs to for universal big genus, is benthic important component part, is the high-quality bait of marine fish, shrimp, crab class.Guo Luo roach at first is applied to cultured fishes, because the development of shrimp culture industry discovers that Guo Luo roach also is that prawn is young, the high-quality living bait of juvenile prawn life stage.
It still is the preferred biological subject that toxicity detects that Guo Luo roach belongs to biology, the part kind has been applied to bio-toxicity and has detected, wherein more comprehensive for the research of Corophium valutator, Environment Canada is with its standard biological subject as the sediment toxicity biological detection.Corophium orientale, Corophium colo, Corophium multisetosum and Corophium insidiosum are also underway as the research of biological subject.
River Guo Luo roach (corophium acherusicum) is that China sea Guo Luo roach belongs to one of 20 kinds of biologies finding, inhabits intertidal zone ooze and silt substrate, in countries such as China, Japan, Australia, New Zealand distribution is arranged all.Still be the biological subject that toxicity detects as food organisms no matter, all very necessary for the research of this kind biology laboratory cultural method.The present invention is specially the indoor cultural method of a kind of amphipoda river Guo Luo roach, cultivates the river Guo Luo roach that obtains and can be used as high-quality bait, is used for economic biology cultures such as marine fish, shrimp, crab class, improves economic biological output; The river Guo Luo roach that cultivation obtains also can be used as the biological subject of the ecological toxicity diagnosis of environmental pollution, the anniversary that guarantees river Guo Luo roach is supplied with, satisfy carrying out of marine sediment, ocean dredging thing and the testing of seawater sample bio-toxicity, solve the problem that environmental pollution biological detection biological subject exists at aspects such as acquisition time, quantity, biological qualities.
Summary of the invention
The present invention is in order to solve the existing in prior technology problem, purpose is to provide the indoor cultural method of a kind of amphipoda river Guo Luo roach, for marine fish, shrimp, crab class etc. provide high-quality bait, for the toxicity testing provides quantity sufficient and colory biological subject.
For achieving the above object, the technical solution used in the present invention is as follows:
An aspect of of the present present invention is: the indoor cultural method of a kind of amphipoda river Guo Luo roach, it comprises the steps:
A. river Guo Luo roach is gathered
Gather the surface deposit about the Guo Luo roach habitat 5cm of river, put into the sieve that mesh is 0.5mm, sift out sediments, collect amphipoda, put it in the container that fills on-the-spot seawater lucifuge oxygenation in the transportation;
B. river Guo Luo roach culture matrix
The clean sediments of culture vessel splendid attire 2-3cm, interpolation seawater volume are 3 times of volume of sediment;
C. river Guo Luo roach is tamed condition
Select river Guo Luo roach individuality, place the culture vessel of getting ready, the unit cell algae bait of throwing something and feeding, oxygenation; Change water every one day, change the unit cell algae bait of throwing something and feeding behind the water, oxygenation; The temperature that rises every day or fall is no more than 3 ℃, rises or the salinity of falling is no more than 3, and domestication is to laboratory long-term cultivation condition gradually;
D. river Guo Luo roach condition of culture
15～25 ℃ of temperature, salinity 22～26, the unit cell algae bait of throwing something and feeding, oxygenation; Change water every one day, change the unit cell algae bait of throwing something and feeding behind the water; 30～50d changes sediments, separates adult and the young.
Concrete, in above-mentioned cultural method, the described culture vessel of step b after the cleaning, is that 10%HCl solution soaked 6 hours at least with concentration, water is rinsed well.
Concrete, in above-mentioned cultural method, step b is described:
Cultivate sediments: gather the collection ground from river Guo Luo roach, through the sieve screening of 0.5mm, remove stone and bulky grain thing, the sediments after sieving is contained in the container, places refrigerator-20 ℃ frozen 24h at least, removes the other biological in the sediments; Frozen sediments is put into 60 ℃ in baking oven to drying or natural air drying, and it is standby to pulverize the back;
Cultivate seawater: its salinity is that coastal seawater is allocated gained through sand filtration, precipitation and 20 μ m silk cover filterings and the running water behind aeration.
Concrete, in above-mentioned cultural method, the volume that changes water described in step c or the d is 2/3 of former interpolation seawater volume.
Concrete, in above-mentioned cultural method, the unit cell algae bait described in step c or the d is following a kind of:
6 * 10 6The Nitzschia closterium minutissima of cell/ml;
6 * 10 5The big flat algae in the Qingdao of cell/ml;
Nitzschia closterium minutissima (6 * 10 6Cell/ml) and the big flat algae (6 * 10 in Qingdao 5Cell/ml) with the equal-volume mixed solution;
Nitzschia closterium minutissima (6 * 10 6Cell/ml) and the big flat algae (6 * 10 in Qingdao 5Cell/ml) with the equal-volume mixed solution, additional synthetic diet (0.1mg/ml); Described synthetic diet be the prawn zoea stage or acanthosoma used synthetic diet.
The present invention is indoor cultivation river Guo Luo roach, it can be grown and breed under the laboratory cultures condition after taming, reach this kind biology can be in the laboratory long-term cultivation, for marine fish, shrimp, crab provide the high-quality living bait, for the toxicity testing provides quantity sufficient and biological colory biological subject.
Embodiment
Following non-limiting example can make those of ordinary skill in the art more fully understand the present invention, but does not limit the present invention in any way.
Embodiment 1
A. river Guo Luo roach is gathered
In morning or afternoon low tide one, select river Guo Luo roach population density higher position, scrape gently with spade and to get 5cm left and right sides surface deposit, putting into mesh is the stainless steel sieve of 0.5mm, sift out sediments, collect amphipoda, put it in the Plastic Drum that fills on-the-spot seawater, avoid illumination, take back the laboratory rapidly; As the need long-distance transport, need oxygenation, to guarantee oxygen abundance in the seawater.
B. river Guo Luo roach culture matrix
In the laboratory, select the square plastic tank of 30 * 20 * 15cm for use; After cleaning culture vessel, soaked at least 6 hours for 10%HCl solution with concentration, rinse well with fresh water then.In container, add clean sediments, clean seawater, standby;
Cultivate sediments: for gathering the collection ground from river Guo Luo roach, through the sieve screening of 0.5mm, remove stone and bulky grain thing, the sediments after sieving is contained in the container, places refrigerator-20 ℃ frozen 24h at least, removes the other biological in the sediments.Frozen sediments is put into 60 ℃ in baking oven to drying or natural air drying, pulverize the back and be used for biological culture.
Cultivate seawater: its salinity is that coastal seawater is allocated gained through sand filtration, precipitation and 20 μ m silk cover filterings and the running water behind aeration.The clean sediments of culture vessel splendid attire is that 2-3cm is thick, and interpolation seawater volume is 3 times of volume of sediment.
C. river Guo Luo roach is tamed condition
Select river Guo Luo roach individuality, place the culture vessel of getting ready, the bait of throwing something and feeding, oxygenation; Changed water every one day, the volume that changes water every other day is 2/3 of former interpolation seawater volume.Change the bait of throwing something and feeding behind the water, oxygenation.Liter every day (falling) temperature is no more than 3 ℃, rises (falling) salinity and is no more than 3, and domestication is to laboratory long-term cultivation condition gradually.
Described bait is: Nitzschia closterium minutissima (6 * 10 6Cell/ml) and the big flat algae (6 * 10 in Qingdao 5Cell/ml) 1: 1 solution of Zu Chenging, additional synthetic diet (0.1mg/ml).
D. river Guo Luo roach condition of culture
Laboratory long-term cultivation condition is: 15～25 ℃ of temperature, salinity 22～26, the bait of throwing something and feeding, oxygenation; Change water every one day, change the bait of throwing something and feeding behind the water; 30～50d changes sediments, separates adult and the young.
Described bait is: Nitzschia closterium minutissima (6 * 10 6Cell/ml) and the big flat algae (6 * 10 in Qingdao 5Cell/ml) 1: 1 solution of Zu Chenging, additional synthetic diet (0.1mg/ml).Described synthetic diet be the prawn zoea stage or acanthosoma used synthetic diet.
Embodiment 2
Basic cultural method is with embodiment 1, and difference is:
The culture vessel of described step b is 20-25L round plastic basin.
Among described step c or the d, the bait of throwing something and feeding is: 6 * 10 6The Nitzschia closterium minutissima of cell/ml.
Embodiment 3
Basic cultural method is with embodiment 1, and difference is:
The culture vessel of described step b is 20-25L round plastic basin.
Among described step c or the d, the bait of throwing something and feeding is: 6 * 10 5The big flat algae in the Qingdao of cell/ml.
Embodiment 4
Basic cultural method is with embodiment 1, and difference is:
The culture vessel of described step b is 20-25L round plastic basin.
Among described step c or the d, the bait of throwing something and feeding is: the big flat algae (6 * 10 in Qingdao 5Cell/ml) and Nitzschia closterium minutissima (6 * 10 6Cell/ml) the two isopyknic mixed liquor.

Claims (5)

1. the indoor cultural method of an amphipoda river Guo Luo roach is characterized in that, comprises the steps:
A. river Guo Luo roach is gathered
Gather the surface deposit about the Guo Luo roach habitat 5cm of river, put into the sieve that mesh is 0.5mm, sift out sediments, collect amphipoda, put it in the container that fills on-the-spot seawater lucifuge oxygenation in the transportation;
B. river Guo Luo roach culture matrix
The clean sediments of culture vessel splendid attire 2-3cm, interpolation seawater volume are 3 times of volume of sediment;
C. river Guo Luo roach is tamed condition
Select river Guo Luo roach individuality, place the culture vessel of getting ready, the unit cell algae bait of throwing something and feeding, oxygenation; Change water every one day, change the unit cell algae bait of throwing something and feeding behind the water, oxygenation; The temperature that rises every day or fall is no more than 3 ℃, rises or the salinity of falling is no more than 3, and domestication is to laboratory long-term cultivation condition gradually;
D. river Guo Luo roach condition of culture
15～25 ℃ of temperature, salinity 22～26, the unit cell algae bait of throwing something and feeding, oxygenation; Change water every one day, change the unit cell algae bait of throwing something and feeding behind the water; 30～50d changes sediments, separates adult and the young.
2. cultural method according to claim 1, it is characterized in that: the described culture vessel of step b after the cleaning, is that 10%HCl solution soaked 6 hours at least with concentration, and water is rinsed well.
3. cultural method according to claim 1 and 2, it is characterized in that: step b is described:
Cultivate sediments: gather the collection ground from river Guo Luo roach, through the sieve screening of 0.5mm, remove stone and bulky grain thing, the sediments after sieving is contained in the container, places refrigerator-20 ℃ frozen 24h at least, removes the other biological in the sediments; Frozen sediments is put into 60 ℃ in baking oven to drying or natural air drying, and it is standby to pulverize the back;
Cultivate seawater: its salinity is that coastal seawater is allocated gained through sand filtration, precipitation and 20 μ m silk cover filterings and the running water behind aeration.
4. cultural method according to claim 3, it is characterized in that: the volume that changes water described in step c or the d is 2/3 of former interpolation seawater volume.
5. according to claim 1 or 4 described cultural methods, it is characterized in that: the unit cell algae bait described in step c or the d is following a kind of:
6 * 10 6The Nitzschia closterium minutissima of cell/ml;
6 * 10 5The big flat algae in the Qingdao of cell/ml;
Nitzschia closterium minutissima (6 * 10 6Cell/ml) and the big flat algae (6 * 10 in Qingdao 5Cell/ml) with the equal-volume mixed solution;
Nitzschia closterium minutissima (6 * 10 6Cell/ml) and the big flat algae (6 * 10 in Qingdao 5Cell/ml) with the equal-volume mixed solution, additional synthetic diet (0.1mg/ml); Described synthetic diet be the prawn zoea stage or acanthosoma used synthetic diet.
CN201210014486.5A 2012-01-17 2012-01-17 The indoor cultural method of a kind of river Guo Luo roach Expired - Fee Related CN103202245B (en)

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Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103454381A (en) * 2013-09-10 2013-12-18 沈阳航空航天大学 Biological diagnosis method of sediment toxicity
CN107691318A (en) * 2017-11-13 2018-02-16 烟台大学 A kind of high-efficient culture method of comprehensive ecological Guo Luo roaches
CN112293308A (en) * 2020-11-19 2021-02-02 广东大麟洋海洋生物有限公司 Golden pomfret breeding method

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US20010045189A1 (en) * 1999-12-30 2001-11-29 Mcneil Roderick J. Shrimp culture system
JP2011244795A (en) * 2010-05-21 2011-12-08 Minamikyushu City Method for producing on land of sand-submerged bivalves, such as littleneck clam

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JP2011244795A (en) * 2010-05-21 2011-12-08 Minamikyushu City Method for producing on land of sand-submerged bivalves, such as littleneck clam

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Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103454381A (en) * 2013-09-10 2013-12-18 沈阳航空航天大学 Biological diagnosis method of sediment toxicity
CN107691318A (en) * 2017-11-13 2018-02-16 烟台大学 A kind of high-efficient culture method of comprehensive ecological Guo Luo roaches
CN107691318B (en) * 2017-11-13 2020-04-17 烟台大学 Comprehensive ecological corophium acherusicum cultivation method
CN112293308A (en) * 2020-11-19 2021-02-02 广东大麟洋海洋生物有限公司 Golden pomfret breeding method

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