CN103202246A - Indoor culturing method for Japanese grandidierella - Google Patents
Indoor culturing method for Japanese grandidierella Download PDFInfo
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- CN103202246A CN103202246A CN2012100160143A CN201210016014A CN103202246A CN 103202246 A CN103202246 A CN 103202246A CN 2012100160143 A CN2012100160143 A CN 2012100160143A CN 201210016014 A CN201210016014 A CN 201210016014A CN 103202246 A CN103202246 A CN 103202246A
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- roach
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- seawater
- grandidierella
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
Abstract
The invention discloses a culturing method for an amphipoda living thing of Japanese grandidierella. The culturing method comprises the following steps of: collecting Japanese grandidierella individuals in the wild and seawater of a sampling area; after a culturing container is cleaned, adding clean sedimentation and cultivation seawater in the container; selecting the Japanese grandidierella individuals, and placing in a prepared culturing container in the condition with daily temperature increase or decrease not more than 3 DEG C and salinity increase or decrease not more than 3, gradually acclimating to a lab long-term culturing condition with a temperature of 20-26 DEG C and the salinity of 10-26, throwing and feeding unialgal baits, and adding synthesized baits; changing water every other day, and throwing and feeding baits after water changing; oxygenizing, and replacing sedimentation in 30-50d (days), and separating adult grandidierella from baby grandidierella. The obtained Japanese grandidierella cultured by the method can be used as alive baits for economic living things of fish, shrimps, crabs and the like as well as test living things for toxicity detection of samples of sea sedimentation, sea dredges and seawater, and the culturing method has broad application prospect.
Description
Technical field
The present invention relates to marine benthos and cultivate, be specially the indoor cultural method of the big chela roach of a kind of amphipoda Japan.
Background technology
Amphipoda is the important component part of marine benthos, is the main source of fish and most of birds food; Kind is many, distribution is wide, has all over the world; Quantity is big, the breeding cycle is short.Amphipoda becomes the preferred biological subject that bio-toxicity detects because of above-mentioned advantage.
But the amphipoda biological subject that is used for the toxicity detection comes from field acquisition mostly, thereby has the problem of aspects such as biological subject acquisition time, quantity, biological quality, causes the toxicity testing to be subjected to very big restriction.For satisfying the needs of toxicity testing, the laboratory anniversary long-term cultivation of amphipoda biological subject is very necessary.
At present the amphipoda biological subject can accomplish in the kind of laboratory long term culture few kinds such as Ampelisca abdita, Leptocheirus plumulosus, Hyalella azteca are only arranged, during the laboratory cultures technology that comprises kinds such as Corophium volutator is constantly being groped.
The big chela roach (Grandidierella japonica) of Japan is under the jurisdiction of the big chela roach of Amphipoda Gammaridea Corophiidae and belongs to, and inhabits intertidal zone ooze and silt substrate.Be found in Japan at first, be world's kind that extensively distributes, all there is report the Atlantic Ocean and pacific rim, in China Bohai Sea, the Huanghai Sea and the East Sea distribution are arranged all, and the anniversary occurs.This kind biology is by U.S. material and the standard biological subject of test association as the sediment toxicity detection.The present invention is specially seawater acclimation and the indoor cultural method of the big chela roach of a kind of amphipoda Japan, the big chela roach of the Japan that cultivation obtains can be used as the biological subject of the ecological toxicity diagnosis of environmental pollution, the anniversary that ensures the big chela roach of Japan is supplied with, satisfy carrying out of marine sediment, ocean dredging thing and the testing of seawater sample bio-toxicity, solve the problem that environmental pollution biological detection biological subject exists at aspects such as acquisition time, quantity, biological qualities.
Summary of the invention
The present invention is in order to solve the existing in prior technology problem, and purpose is to provide the cultural method of the big chela roach of the biological Japan of a kind of amphipoda, provides quantity sufficient and colory biological subject, satisfies the needs of toxicity testing.
The present invention is domestication and cultivates the big chela roach of Japan, make its growth and breeding under the optimal culture condition of laboratory after taming, reach this kind biology can be in the laboratory long-term cultivation, guarantee that Japan supplies with in the big chela roach laboratory anniversary, satisfy the toxicity testing to the requirement of biological subject acquisition time, quantity and biological quality, ensure carrying out smoothly of toxicity testing.
For achieving the above object, the technical solution used in the present invention is as follows:
An aspect of of the present present invention is: the indoor cultural method of the big chela roach of a kind of amphipoda Japan, it comprises the steps:
A. the big chela roach of Japan is gathered
Gather the surface deposit about the big chela roach habitat 5cm of Japan, put into the sieve that mesh is 0.5mm, sift out sediments, collect amphipoda, put it in the container that fills on-the-spot seawater lucifuge oxygenation in the transportation;
B. the big chela roach culture matrix of Japan
The clean sediments of culture vessel splendid attire 2-3cm, interpolation seawater volume are 3 times of volume of sediment;
C. the big chela roach of Japan is tamed condition
Select the big chela roach individuality of Japan, place the culture vessel of getting ready, the unit cell algae bait of throwing something and feeding, oxygenation; Change water every one day, change the unit cell algae bait of throwing something and feeding behind the water, oxygenation; The temperature that rises every day or fall is no more than 3 ℃, rises or the salinity of falling is no more than 3, and domestication is to laboratory long-term cultivation condition gradually;
D. the big chela roach condition of culture of Japan
20～26 ℃ of temperature, salinity 10～26, the unit cell algae bait of throwing something and feeding, oxygenation; Change water every one day, change the unit cell algae bait of throwing something and feeding behind the water; 30～50d changes sediments, separates adult and the young.
Concrete, in above-mentioned cultural method, the described culture vessel of step b after the cleaning, is that 10%HCl solution soaked 6 hours at least with concentration, water is rinsed well.
Concrete, in above-mentioned cultural method, step b is described:
Cultivate sediments: gather the collection ground from the big chela roach of Japan, through the sieve screening of 0.5mm, remove stone and bulky grain thing, the sediments after sieving is contained in the container, places refrigerator-20 ℃ frozen 24h at least, removes the other biological in the sediments; Frozen sediments is put into 60 ℃ in baking oven to drying or natural air drying, and it is standby to pulverize the back;
Cultivate seawater: its salinity is that coastal seawater is allocated gained through sand filtration, precipitation and 20 μ m silk cover filterings and the running water behind aeration.
Concrete, in above-mentioned cultural method, the volume that changes water described in step c or the d is 2/3 of former interpolation seawater volume.
Concrete, in above-mentioned cultural method, the unit cell algae bait described in step c or the d is following a kind of:
6 * 10
6The Nitzschia closterium minutissima of cell/ml;
6 * 10
5The big flat algae in the Qingdao of cell/ml;
Nitzschia closterium minutissima (6 * 10
6Cell/ml) and the big flat algae (6 * 10 in Qingdao
5Cell/ml) with the equal-volume mixed solution;
Nitzschia closterium minutissima (6 * 10
6Cell/ml) and the big flat algae (6 * 10 in Qingdao
5Cell/ml) with the equal-volume mixed solution, additional synthetic diet (0.1mg/ml); Described synthetic diet be the prawn zoea stage or acanthosoma used synthetic diet.
The present invention is the big chela roach of indoor cultivation Japan, it can be grown and breed under the laboratory cultures condition after taming, reach this kind biology can be in the laboratory long-term cultivation, for marine fish, shrimp, crab provide the high-quality living bait, for the toxicity testing provides quantity and biological colory biological subject.
Embodiment
Following non-limiting example can make those of ordinary skill in the art more fully understand the present invention, but does not limit the present invention in any way.
Embodiment 1
A. the big chela roach of Japan is gathered
In morning or afternoon low tide one, select the big chela roach population density higher position of Japan, scrape gently with spade and to get 5cm left and right sides surface deposit, putting into mesh is the stainless steel sieve of 0.5mm, sift out sediments, collect amphipoda, put it in the Plastic Drum that fills on-the-spot seawater, avoid illumination, take back the laboratory rapidly; As the need long-distance transport, need oxygenation, to guarantee oxygen abundance in the seawater.
B. the big chela roach culture matrix of Japan
In the laboratory, select the square plastic tank of 30 * 20 * 15cm for use; After cleaning culture vessel, soaked at least 6 hours for 10%HCl solution with concentration, rinse well with fresh water then.In container, add clean sediments, clean seawater, standby;
Cultivate sediments: for gathering the collection ground from the big chela roach of Japan, through the sieve screening of 0.5mm, remove stone and bulky grain thing, the sediments after sieving is contained in the container, places refrigerator-20 ℃ frozen 24h at least, removes the other biological in the sediments.Frozen sediments is put into 60 ℃ in baking oven to drying or natural air drying, pulverize the back and be used for biological culture.
Cultivate seawater: its salinity is that coastal seawater is allocated gained through sand filtration, precipitation and 20 μ m silk cover filterings and the running water behind aeration.It is that 2-3cm is thick that the culture vessel splendid attire is cultivated sediments, and interpolation seawater volume is 3 times of volume of sediment.
C. the big chela roach of Japan is tamed condition
Select the big chela roach individuality of Japan, place the culture vessel of getting ready, the bait of throwing something and feeding, oxygenation; Changed water every one day, the volume that changes water every other day is 2/3 of former interpolation seawater volume.Change the bait of throwing something and feeding behind the water, oxygenation.Liter every day (falling) temperature is no more than 3 ℃, rises (falling) salinity and is no more than 3, and domestication is to laboratory long-term cultivation condition gradually.
Described bait is: Nitzschia closterium minutissima (6 * 10
6Cell/ml) and the big flat algae (6 * 10 in Qingdao
5Cell/ml) 1: 1 solution of Zu Chenging, additional synthetic diet (0.1mg/ml).
D. the big chela roach condition of culture of Japan
Laboratory long-term cultivation condition is: 20 ± 1 ℃ of temperature, salinity 25 ± 1, the bait of throwing something and feeding, oxygenation; Change water every one day, change the bait of throwing something and feeding behind the water; 30～50d changes sediments, separates adult and the young.
Described bait is: Nitzschia closterium minutissima (6 * 10
6Cell/ml) and the big flat algae (6 * 10 in Qingdao
5Cell/ml) 1: 1 solution of Zu Chenging, additional synthetic diet (0.1mg/ml).Described synthetic diet be the prawn zoea stage or acanthosoma used synthetic diet.
Embodiment 2
Basic cultural method is with embodiment 1, and difference is:
The culture vessel of described step b is 20-25L round plastic basin.
Among described step c or the d, the bait of throwing something and feeding is: 6 * 10
6The Nitzschia closterium minutissima of cell/ml.
Embodiment 3
Basic cultural method is with embodiment 1, and difference is:
The culture vessel of described step b is 20-25L round plastic basin.
Among described step c or the d, the bait of throwing something and feeding is: 6 * 10
5The big flat algae in the Qingdao of cell/ml.
Embodiment 4
Basic cultural method is with embodiment 1, and difference is:
The culture vessel of described step b is 20-25L round plastic groove.
Among described step c or the d, the bait of throwing something and feeding is: the big flat algae (6 * 10 in Qingdao
5Cell/ml) and Nitzschia closterium minutissima (6 * 10
6Cell/ml) the two isopyknic mixed liquor.
Embodiment 5
Basic cultural method is with embodiment 1, and difference is:
The culture vessel of described step b is 20-25L round plastic basin.
Described cultivation temperature can be 20～26 ℃, accelerate growth and the breeding of the big chela roach of Japan by the rising temperature, but temperature raising every day is no more than 3 ℃.
Claims (5)
1. the indoor cultural method of the big chela roach of amphipoda Japan is characterized in that, comprises the steps:
A. the big chela roach of Japan is gathered
Gather the surface deposit about the big chela roach habitat 5cm of Japan, put into the sieve that mesh is 0.5mm, sift out sediments, collect amphipoda, put it in the container that fills on-the-spot seawater lucifuge oxygenation in the transportation;
B. the big chela roach culture matrix of Japan
The clean sediments of culture vessel splendid attire 2-3cm, interpolation seawater volume are 3 times of volume of sediment;
C. the big chela roach of Japan is tamed condition
Select the big chela roach individuality of Japan, place the culture vessel of getting ready, the unit cell algae bait of throwing something and feeding, oxygenation; Change water every one day, change the unit cell algae bait of throwing something and feeding behind the water, oxygenation; The temperature that rises every day or fall is no more than 3 ℃, rises or the salinity of falling is no more than 3, and domestication is to laboratory long-term cultivation condition gradually;
D. the big chela roach condition of culture of Japan
20～26 ℃ of temperature, salinity 10～26, the unit cell algae bait of throwing something and feeding, oxygenation; Change water every one day, change the unit cell algae bait of throwing something and feeding behind the water; 30～50d changes sediments, separates adult and the young.
2. cultural method according to claim 1, it is characterized in that: the described culture vessel of step b after the cleaning, is that 10%HCl solution soaked 6 hours at least with concentration, and water is rinsed well.
3. cultural method according to claim 1 and 2, it is characterized in that: step b is described:
Cultivate sediments: gather the collection ground from the big chela roach of Japan, through the sieve screening of 0.5mm, remove stone and bulky grain thing, the sediments after sieving is contained in the container, places refrigerator-20 ℃ frozen 24h at least, removes the other biological in the sediments; Frozen sediments is put into 60 ℃ in baking oven to drying or natural air drying, and it is standby to pulverize the back;
Cultivate seawater: its salinity is that coastal seawater is allocated gained through sand filtration, precipitation and 20 μ m silk cover filterings and the running water behind aeration.
4. cultural method according to claim 3, it is characterized in that: the volume that changes water described in step c or the d is 2/3 of former interpolation seawater volume.
5. according to claim 1 or 4 described cultural methods, it is characterized in that: the unit cell algae bait described in step c or the d is following a kind of:
6 * 10
6The Nitzschia closterium minutissima of cell/ml;
6 * 10
5The big flat algae in the Qingdao of cell/ml;
Nitzschia closterium minutissima (6 * 10
6Cell/ml) and the big flat algae (6 * 10 in Qingdao
5Cell/ml) with the equal-volume mixed solution;
Nitzschia closterium minutissima (6 * 10
6Cell/ml) and the big flat algae (6 * 10 in Qingdao
5Cell/ml) with the equal-volume mixed solution, additional synthetic diet (0.1mg/ml); Described synthetic diet be the prawn zoea stage or acanthosoma used synthetic diet.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210016014.3A CN103202246B (en) | 2012-01-17 | 2012-01-17 | The indoor cultural method of a kind of Japan large chela roach |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210016014.3A CN103202246B (en) | 2012-01-17 | 2012-01-17 | The indoor cultural method of a kind of Japan large chela roach |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103202246A true CN103202246A (en) | 2013-07-17 |
| CN103202246B CN103202246B (en) | 2015-08-26 |
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|---|---|---|---|
| CN201210016014.3A Expired - Fee Related CN103202246B (en) | 2012-01-17 | 2012-01-17 | The indoor cultural method of a kind of Japan large chela roach |
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20110045189A1 (en) * | 2008-04-19 | 2011-02-24 | Dingzhong Huang | Composition for treating hard surface and use thereof, coating formed thereby and hard surface material with the coating |
| JP2011244795A (en) * | 2010-05-21 | 2011-12-08 | Minamikyushu City | Method for producing on land of sand-submerged bivalves, such as littleneck clam |
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2012
- 2012-01-17 CN CN201210016014.3A patent/CN103202246B/en not_active Expired - Fee Related
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20110045189A1 (en) * | 2008-04-19 | 2011-02-24 | Dingzhong Huang | Composition for treating hard surface and use thereof, coating formed thereby and hard surface material with the coating |
| JP2011244795A (en) * | 2010-05-21 | 2011-12-08 | Minamikyushu City | Method for producing on land of sand-submerged bivalves, such as littleneck clam |
Non-Patent Citations (4)
| Title |
|---|
| 王睿睿等: "实验室培养日本大螯蜚f1代的生活史", 《海洋环境科学》 * |
| 闫启仑等: "实验室培养的端足类日本大螯蜚F1代对Cd的急性毒性响应", 《海洋环境科学》 * |
| 陈红星等: "室内培养底栖端足类日本大螯蜚饵料研究", 《海洋环境科学》 * |
| 韩明辅等: "盐度对端足类日本大螯蜚生长发育的影响", 《海洋科学环境》 * |
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