CN103196975B - Detection apparatus and detection method of medical relative oxidation reduction potential - Google Patents
Detection apparatus and detection method of medical relative oxidation reduction potential Download PDFInfo
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- CN103196975B CN103196975B CN201310069751.4A CN201310069751A CN103196975B CN 103196975 B CN103196975 B CN 103196975B CN 201310069751 A CN201310069751 A CN 201310069751A CN 103196975 B CN103196975 B CN 103196975B
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- 238000000034 method Methods 0.000 claims description 15
- 239000000284 extract Substances 0.000 claims description 3
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- 239000000243 solution Substances 0.000 description 30
- 210000002381 plasma Anatomy 0.000 description 12
- BASFCYQUMIYNBI-UHFFFAOYSA-N platinum Chemical compound [Pt] BASFCYQUMIYNBI-UHFFFAOYSA-N 0.000 description 12
- 238000012360 testing method Methods 0.000 description 8
- 210000004369 blood Anatomy 0.000 description 6
- 239000008280 blood Substances 0.000 description 6
- 229910052697 platinum Inorganic materials 0.000 description 6
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- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 3
- BPKIGYQJPYCAOW-FFJTTWKXSA-I calcium;potassium;disodium;(2s)-2-hydroxypropanoate;dichloride;dihydroxide;hydrate Chemical compound O.[OH-].[OH-].[Na+].[Na+].[Cl-].[Cl-].[K+].[Ca+2].C[C@H](O)C([O-])=O BPKIGYQJPYCAOW-FFJTTWKXSA-I 0.000 description 3
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- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 description 2
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- ZZZCUOFIHGPKAK-UHFFFAOYSA-N D-erythro-ascorbic acid Natural products OCC1OC(=O)C(O)=C1O ZZZCUOFIHGPKAK-UHFFFAOYSA-N 0.000 description 1
- HTTJABKRGRZYRN-UHFFFAOYSA-N Heparin Chemical compound OC1C(NC(=O)C)C(O)OC(COS(O)(=O)=O)C1OC1C(OS(O)(=O)=O)C(O)C(OC2C(C(OS(O)(=O)=O)C(OC3C(C(O)C(O)C(O3)C(O)=O)OS(O)(=O)=O)C(CO)O2)NS(O)(=O)=O)C(C(O)=O)O1 HTTJABKRGRZYRN-UHFFFAOYSA-N 0.000 description 1
- WHXSMMKQMYFTQS-UHFFFAOYSA-N Lithium Chemical compound [Li] WHXSMMKQMYFTQS-UHFFFAOYSA-N 0.000 description 1
- 206010063837 Reperfusion injury Diseases 0.000 description 1
- 229910021607 Silver chloride Inorganic materials 0.000 description 1
- CDBYLPFSWZWCQE-UHFFFAOYSA-L Sodium Carbonate Chemical compound [Na+].[Na+].[O-]C([O-])=O CDBYLPFSWZWCQE-UHFFFAOYSA-L 0.000 description 1
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- 230000001575 pathological effect Effects 0.000 description 1
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- 239000012286 potassium permanganate Substances 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
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- 229910052709 silver Inorganic materials 0.000 description 1
- 239000004332 silver Substances 0.000 description 1
- HKZLPVFGJNLROG-UHFFFAOYSA-M silver monochloride Chemical compound [Cl-].[Ag+] HKZLPVFGJNLROG-UHFFFAOYSA-M 0.000 description 1
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- Investigating Or Analyzing Non-Biological Materials By The Use Of Chemical Means (AREA)
Abstract
The invention discloses a detection apparatus and a detection method of medical relative oxidation reduction potential. The detection apparatus comprises a detecting tube and an elastic sealing plug mounted at a top opening of the detecting tube body, wherein the elastic sealing plug is provided with a first through hole and a second through hole, a detecting electrode passes through the first through hole, a communicating fine pipe passes through the second through hole, and a valve is mounted at one end of the communicating fine pipe located outside the detecting tube. The detection method comprises: (1) vacuumizing the detecting tube through the communicating fine pipe, adding reference liquid into the detecting tube, closing the valve, and detecting an oxidation reduction potential of the reference liquid; (2) opening the valve, adding to-be-detected liquid into the reference liquid, closing the valve, uniformly mixing to obtain a mixed liquid, and detecting an oxidation reduction potential of the mixed liquid; and (3) calculating a difference between the oxidation reduction potentials of the mixed liquid and the reference liquid to obtain an oxidation reduction potential of the to-be-detected liquid relative to the reference liquid.
Description
Technical field
The invention belongs to clinical medicine detection field, relate in particular to a kind of pick-up unit and detection method of medical relative oxidation-reduction potential.
Background technology
Oxidation-reduction potential (Oxidation Reduction Potential, ORP) is a composite target of medium (comprising soil, natural water, nutrient culture media etc.) environmental baseline, and it has characterized the relative extent of medium oxidisability or reductibility.
For a water body, often there is multiple oxidation-reduction pair, form complicated redox system.The oxidation-reduction potential of water body is the synthesis result of many oxide matter and reducing substances generation redox reaction, is used for reflecting macroscopical oxidation-reducibility that in aqueous solution, all substances show.This index contributes to understand the electrochemical characteristic of water body, the character of analyzing water body.
The measurement mechanism of oxidation-reduction potential is made up of ORP electrode and potential difference meter conventionally, and ORP electrode is a kind of electrode that can carry out Electron absorption or release on its sensitive layer surface, and this sensitive layer is a kind of inert metal, conventionally makes of platinum and Jin Lai.Contrast electrode is silver/silver chloride electrode or saturated calomel electrode.
Traditional measurement oxidation-reduction potential generally adopts platinum electrode direct measuring method or depolarization determination method etc.Platinum electrode direct measuring method directly inserts in medium and measures by platinum electrode and contrast electrode.But in the time measuring weak equilibrium system, due to platinum electrode nisi inertia, its surface can form oxide film or adsorb other materials, thereby affects the electron exchange speed of each oxidation-reduction pair on platinum electrode, makes the foundation of equilibrium potential very slow.
Adopt the processing such as beveled electrodes so traditional assay method before each mensuration more, to ensure the stability of mensuration state of electrode.And traditional open, semi open model assay method, due to the impact of extraneous oxygen etc., inevitably will affect the stability of measurement result.
But while mensuration, also there is no special pick-up unit, is generally to select the ready-made instrument in laboratory to assemble according to the requirement of correlation method to obtain pick-up unit.Not only complex structure, complex steps, and often run into the problem that poorly sealed or water sample can not proper flow, cannot the real conditions of simulated water sample in actual environment, and polarization effect very easily occurs electrode, produces unnecessary error.
Notification number is that the Chinese patent literature of CN202083664U discloses a kind of anti-electrode polarization effect ORP detection bottle, bottle top end opening is also provided with gland bonnet, bottle side wall is provided with measurement scale line, bottle internal upper part 1/5th places are provided with screen pack, and 1/5th places, bottom are provided with separation net.Gland bonnet is provided with 2～5 holes, for laying temperature sensor and detecting electrode.
For the application of medical domain, because human internal environment is the liquid environment of sealing, and existing oxidation reduction potential determination method complicated operation, electrode before mensuration is processed loaded down with trivial details, stable electrode condition is difficult for holding, and accidental error is larger, is not suitable with clinical medical timely monitoring and measuring application.
Summary of the invention
The invention provides a kind of pick-up unit of medical relative oxidation-reduction potential, utilize this device under the mobile state of sealing, the oxidation-reduction potential of solution to be measured, avoid external environment impact, increased the stability of measuring.
A kind of pick-up unit of medical relative oxidation-reduction potential, comprise detector tube and the resilient seal plug that is arranged on detector tube body top end opening, described resilient seal is provided with the first through hole and the second through hole beyond the Great Wall, in the first through hole, be penetrated with detecting electrode, in the second through hole, be penetrated with connection tubule, one end that connection tubule is positioned at outside detector tube is provided with valve.
Detecting electrode and the first through hole, be communicated with tubule and the second through hole is sealed and matched respectively, ensure the sealed environment in detector tube.
Described valve is preferably three-way cock, and a port of this three-way cock is connected with connection tubule, and all the other ports are connected with feed tube.Usually, select syringe to add solution in detector tube, therefore, the caliber of feed tube should adapt with syringe.The feed tube of two ports is respectively used to add reference liquid and solution to be measured, avoids syringe mutually to pollute.
According to concrete needs, can select to have the switch of different number ports, but the number of port is at least two.Usually, three-way cock can meet basic testing requirement.
When use, at the bottom of detecting electrode should being stretched into the pipe of detector tube, so use a small amount of solution can infiltrate detecting electrode as far as possible, save solution to be measured; But detecting electrode can not contact with at the bottom of the pipe of detector tube, in order to avoid detecting electrode is caused damage, affects testing result.
The present invention should provide a kind of method of utilizing described pick-up unit to detect relative oxidation-reduction potential, comprising:
(1) after detector tube being vacuumized by connection tubule, in detector tube, add reference liquid, valve-off, the oxidation-reduction potential of detection reference liquid;
(2) open valve, in reference liquid, add solution to be measured, valve-off, mixes and obtains mixed liquor, detects the oxidation-reduction potential of mixed liquor;
(3) difference of the calculating oxidation-reduction potential of mixed liquor and the oxidation-reduction potential of reference liquid, obtains the oxidation-reduction potential of solution phase to be measured for reference liquid.
Preferably, adopt syringe to vacuumize.Syringe is easy to obtain, easy and simple to handle.Before each detection, all detector tube is vacuumized, avoid the impact on SOLUTION PROPERTIES of residual air in detector tube, ensure the accuracy of testing result.
Detected temperatures is preferably 23～25 DEG C, more preferably 25 DEG C.Approach with room temperature, be easy to control.
The volume ratio of reference liquid and solution to be measured is used the method requirement of measuring electrode according to reality, require to regulate according to liquid measure.
While detecting oxidation-reduction potential, detector tube is placed on horizontal shaking table and is slowly run, rotating speed is preferably 20～30r/min, more preferably 20r/min.So can accelerate the reaction velocity of solution and detecting electrode.Rotate and appropriate to the occasion detector tube is vertically placed, in order to avoid affect the liquid level requirement of the mensuration liquid liquid measure in detector tube.
Pick-up unit of the present invention and method are specially adapted to clinical medicine domain, the liquid environment in human body are carried out to the detection of relative oxidation-reduction potential, to characterize its redox state with respect to reference liquid, carry out the quantification monitoring of vivo oxidation reducing condition.When mensuration, relatively different body fluid should be selected the close reference liquid of physiological property with it.
Because blood is the main carriers of carrying various metabolic products, be the important liquid environment that regulates metabolism in body, the redox state of blood is the redox state of the interior liquid environment of antimer totally.Therefore in clinical medicine, often select blood plasma as detecting sample (using Lactated Ringer'S Solution as reference liquid).Blood plasma has been got rid of the impact of cell component etc., is conducive to stability, the reliability measured.
Compared with prior art, beneficial effect of the present invention is:
(1) pick-up unit of the present invention is measured the oxidation-reduction potential of solution under the mobile state of sealing, has avoided the impact of the factors such as the oxygen in external environment, has increased the Stability and veracity of testing result;
(2) detection method of the present invention utilizes the relative determination value of reference liquid as quantizating index, the accidental error having caused because detecting electrode is inconsistent while having avoided each mensuration.
Brief description of the drawings
Fig. 1 is the structural representation of the pick-up unit of the medical relative oxidation-reduction potential of the present invention;
Fig. 2 is the dynamic monitor result figure of detection method of the present invention to burn patient blood plasma; Horizontal ordinate represent blood plasma take from patient burn after the 0th, 1,3,5,7 day and while leaving hospital.
Embodiment
Below in conjunction with the drawings and specific embodiments, technical scheme of the present invention is described in further detail.
Embodiment 1
The pick-up unit of medical relative oxidation-reduction potential as shown in Figure 1, comprises detector tube 1, is arranged on the resilient seal plug 2 of detector tube 1 body top end opening.
Resilient seal plug 2 is provided with in the first through hole 21 and the second through hole 22, the first through holes 21 and is penetrated with in detecting electrode 3, the second through holes 22 and is penetrated with and is communicated with tubule 4, and one end that connection tubule 4 is positioned at outside detector tube 1 is provided with three-way cock 51.One of them port of three-way cock 51 is connected with connection tubule 4, and two other port connects respectively feed tube 52, feed tube 53.
When use, at the bottom of detecting electrode 3 being extended as far as possible to the pipe of detector tube 1, ensure that detecting electrode 3 fully immerses in solution, also can detect in the time only having a small amount of solution in detector tube 1.The degree that stretches into of detecting electrode 3, not to be advisable with contacting at the bottom of the pipe of detector tube 1, in order to avoid detecting electrode 3 is caused to damage, affects testing result.
Usually, adopt syringe that solution is added in detector tube 1, feed tube 52, feed tube 53 are respectively used to add reference liquid and solution to be measured, avoid drawing between the syringe of corresponding solution and mutually pollute.
In this embodiment, detecting electrode 3 adopts the ORP electrode 31 that Italian Kim Yong-jin model is HI3131B.
While carrying out the detection of relative oxidation-reduction potential, first adjust ORP electrode 31, be communicated with the relative fixed position of tubule 4 on resilient seal plug 2, then resilient seal plug 2 is installed in detector tube 1 body top end opening;
Adopt syringe that the air in detector tube 1 is extracted out by three-way cock 51, add reference liquid from feed tube 52 through being communicated with tubule 4, adopt syringe extract detector tube 1 out and be communicated with the residual air in tubule 4, close three-way cock 51; Pick-up unit is vertically placed on shaking table and is slowly run, and rotating speed is 20r/min, to accelerate detection rates; Utilize ORP electrode 31 to detect oxidation-reduction potential the record of reference liquid;
Open three-way cock 51, add solution to be measured from feed tube 53 through being communicated with tubule 4, adopt syringe extract detector tube 1 out and be communicated with the residual air in tubule 4, close three-way cock 51; Solution to be measured and reference liquid are mixed and obtain mixed liquor, detect oxidation-reduction potential the record of mixed liquor;
Calculate the difference of the oxidation-reduction potential of mixed liquor and the oxidation-reduction potential of reference liquid, obtain the oxidation-reduction potential of solution phase to be measured pair and reference liquid, in order to indicate the redox state of solution to be measured.
Embodiment 2
Below taking burn patient blood plasma (plasma) as testing sample, with potassium permanganate (KMnO
4), vitamin C (Vc) is control sample, with Lactated Ringer'S Solution (the lactated ringer's solution of identical with blood plasma pH (through adding the medical 5% soda solution modulation of trace), LR) be reference liquid, to the Stability and veracity of pick-up unit of the present invention and detection method.
The acquisition process of burn patient blood plasma is:
Patient temperature is controlled at about 37-38 DEG C, the associated treatment such as row antioxidant not in half an hour before blood sampling, and row arterial blood gas analysis checks simultaneously, and confirm after Non Apparent Abnormality, adopt negative pressure of vacuum to get blood vessel (BD company product in the stipulated time in the morning, heparin lithium anti-freezing), get the about 2-3mL of femoral vein blood; Under normal temperature, the centrifugal 5min of 2500r/min, gets supernatant blood plasma, injects another airtight identical negative pressure of vacuum and gets in blood vessel, stand-by (can-80 degree preserve).
Detection method is with identical described in embodiment 1, and detection system and testing result are as shown in table 1.
The accuracy test of table 1 detection method of the present invention
From table 1, with LR solution phase ratio, KMnO
4the Δ ORP of LR solution is-231mV to show KMnO relatively
4lR solution is oxidation state relatively, with KMnO
4for the general knowledge of oxygenant conforms to; With LR solution phase ratio, the Δ ORP of the relative LR solution of Vc is 323mV, shows that the relative LR solution of Vc is reduction-state, and the general knowledge that is reductive agent with Vc conforms to.Illustrate that detection method of the present invention has galvanochemistry accuracy.
Embodiment 3
Adopt pick-up unit and the detection method of embodiment 1, adopt the detection system of embodiment 2, under without condition (instant detection with after-80 DEG C of preservations of spending the night detected), the relative oxidation-reduction potential of plasma sample to be measured is carried out to 10 continuous detecting (detecting under 25 DEG C of room temperatures), testing result is in table 2.
Table 2 detection method stability test of the present invention
From table 2,10 times continuous measurement result has shown less value for coefficient of variation, has shown that assay method has good stability.
Embodiment 4
Adopt pick-up unit and the detection method of embodiment 1, adopt the detection system of embodiment 2, the plasma specimen early stage to the course of disease of 23 routine Severe Burn Patients carries out clinical practice dynamic monitoring, and monitoring result as shown in Figure 2.
As seen from Figure 2, in the early stage plasma specimen clinical practice dynamic monitoring of the course of disease of 23 routine Severe Burn Patients, show the dynamic change of patient's oxidative stress status: in early days because shock stage changes the generation (increase of blood oxygen voltinism) that the ischemical reperfusion injury causing causes oxidative stress, and after active treatment, the performance of go down (oxidisability of blood gradually reduces) of oxidative stress.Verify the consistance of this assay method result and clinical pathologic characteristic.Show the feasibility of the practical application of this assay method.
Claims (6)
1. the detection method of a medical relative oxidation-reduction potential, it is characterized in that, the pick-up unit of the medical relative oxidation-reduction potential adopting comprises detector tube (1) and is arranged on the resilient seal plug (2) of detector tube (1) body top end opening, described resilient seal plug (2) is provided with the first through hole (21) and the second through hole (22), in the first through hole (21), be penetrated with detecting electrode (3), in the second through hole (22), be penetrated with and be communicated with tubule (4), connection tubule (4) is positioned at the outer one end of detector tube (1) valve (5) is installed, described valve (5) is three-way cock (51), and a port of this three-way cock (51) is connected with connection tubule (4), and all the other ports are connected with feed tube (52,53),
The method of the relative oxidation-reduction potential of described detection comprises:
(1) after detector tube (1) being vacuumized by connection tubule (4), in detector tube (1), add reference liquid, valve-off (5), the oxidation-reduction potential of detection reference liquid;
(2) open valve (5), in reference liquid, add solution to be measured, valve-off (5), mixes and obtains mixed liquor, detects the oxidation-reduction potential of mixed liquor;
(3) difference of the calculating oxidation-reduction potential of mixed liquor and the oxidation-reduction potential of reference liquid, obtains the oxidation-reduction potential of solution phase to be measured for reference liquid.
2. the method for claim 1, is characterized in that, adopts syringe to vacuumize, and before each mensuration, extracts the gas of detector tube capacity out.
3. the method for claim 1, is characterized in that, detected temperatures is 23～25 DEG C.
4. method as claimed in claim 3, is characterized in that, detected temperatures is 25 DEG C.
5. the method for claim 1, is characterized in that, while detecting oxidation-reduction potential, detector tube is placed on horizontal shaking table and is slowly run, and rotating speed is 20～30r/min.
6. method as claimed in claim 5, is characterized in that, while detecting oxidation-reduction potential, detector tube is placed on horizontal shaking table and is slowly run, and rotating speed is 20r/min.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201310069751.4A CN103196975B (en) | 2013-03-05 | 2013-03-05 | Detection apparatus and detection method of medical relative oxidation reduction potential |
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|---|---|---|---|
| CN201310069751.4A CN103196975B (en) | 2013-03-05 | 2013-03-05 | Detection apparatus and detection method of medical relative oxidation reduction potential |
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| CN103196975B true CN103196975B (en) | 2014-11-05 |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN202083664U (en) * | 2011-01-11 | 2011-12-21 | 南开大学 | Oxidation reduction potential (ORP) detecting bottle capable of preventing electrode polarization effect |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| JP2004053613A (en) * | 2002-07-19 | 2004-02-19 | Shotaro Oka | Oxidation-reduction potential measuring device |
| US8709709B2 (en) * | 2007-05-18 | 2014-04-29 | Luoxis Diagnostics, Inc. | Measurement and uses of oxidative status |
| JP5157880B2 (en) * | 2008-12-22 | 2013-03-06 | 東亜ディーケーケー株式会社 | Electrode inspection method for redox potential measuring device and standard solution for electrode inspection of redox potential measuring device |
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| CN202083664U (en) * | 2011-01-11 | 2011-12-21 | 南开大学 | Oxidation reduction potential (ORP) detecting bottle capable of preventing electrode polarization effect |
Non-Patent Citations (1)
| Title |
|---|
| 苏有勇.氧化还原电位的测定.《沼气发酵检测技术》.2011, * |
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