CN103194526A - Childcare institution disinfection effect supervision and sampling neutralizing liquid - Google Patents
Childcare institution disinfection effect supervision and sampling neutralizing liquid Download PDFInfo
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- CN103194526A CN103194526A CN2012100016299A CN201210001629A CN103194526A CN 103194526 A CN103194526 A CN 103194526A CN 2012100016299 A CN2012100016299 A CN 2012100016299A CN 201210001629 A CN201210001629 A CN 201210001629A CN 103194526 A CN103194526 A CN 103194526A
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- disinfection
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- Apparatus For Disinfection Or Sterilisation (AREA)
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Abstract
The invention relates to a childcare institution disinfection effect supervision and sampling neutralizing liquid used for supervising and evaluating disinfection factor true bactericidal and bacteriostatic effects of a childcare institution disinfection effect. The neutralizing liquid is used for evaluating childcare institution object surface disinfection effect. A disinfection action time and a preset time of the contact of a disinfection liquid and microbes are strictly controlled. When the time is reached, the action is instantly released, such that a childcare institution object surface disinfection liquid disinfection effect precise data can be accurately obtained.
Description
Technical field the present invention relates to a kind of nurseries and kindergartens mechanism sterilisation effect supervision sampling neutralizer.Be used for supervision and estimate the true sterilization of disinfection factor and the bacteriostatic action of nurseries and kindergartens mechanism sterilisation effect.Estimate the disinfection of nurseries and kindergartens mechanism body surface, strict grasp disinfection time and thimerosal and microorganism touched after predetermined time, remove its effect immediately, so just can accurately obtain the precise information of nurseries and kindergartens mechanism surface disinfection liquid action effect; Must not contain remaining thimerosal in the sample after taking to sterilize, prevent that microorganism continues to be subjected to the effect of thimerosal in recovering to cultivate, and go out the sterilization logarithmic value of school falseness.Therefore, design the experiment that nurseries and kindergartens mechanism surface disinfection effect is estimated in a supervision, the reliable method of removing remaining thimerosal all must be arranged.Do not adopt the experiment of removing remaining thimerosal measure, should think serious mistake in design, the result who draws according to such experiment is insecure.The chemistry neutralisation is the thimerosal method of at present the most frequently used removal remnants, and its advantage is that method is simple, and is easy to use, reliable for effect.Nurseries and kindergartens mechanism body surface sample solution, usefulness is the disinfection that physiological saline or phosphate buffered saline (PBS) are used for estimating medical treatment mostly, the experimental data of reporting, 60% is null value, this just makes data reliability to increase greatly.The laboratory disconnects with supervision sampling, the thimerosal neutralizing agent that the laboratory is considered, and common have acid, alkali, absorption, dilution, centrifugal a, filtration etc.The above-mentioned screening of all passing through strictness also can't not determined its working concentration; Corresponding thimerosal do not had solid neutralizing effect; Neutralizing agent itself or with the product of thimerosal reaction the experiment microorganism is had killing or suppress the effect of its growth; To cultivating composition destruction is arranged, also influence its physical behavior.The different varieties sterilizing agent that uses along with nurseries and kindergartens mechanism also is to be badly in need of capturing to the research of neutralizing agent.Disinfection experiment and worker supervision wish to have a kind of nurseries and kindergartens mechanism sterilisation effect supervision sampling neutralizer that can overcome above-mentioned neutralizing agent deficiency always.
Background technology kindergarten nurseries and kindergartens mechanism is the place of children's collective livelihood, study, activity, and it is the important factor that causes hand foot mouth disease and other infectious disease transmissions that its sanitary condition and children's hand and body surface pollute.For strengthening the hygienic quality management to nurseries and kindergartens mechanism, understand nurseries and kindergartens mechanism disinfecting situation, improve child's health care level of starting going to a nursery, disease prevention and sanitary inspection department all will regularly carry out sample detecting to nurseries and kindergartens mechanism disinfecting situation by the requirement of the Ministry of Health.Purpose of the present invention is to provide a kind of deficiency that can overcome existing sample solution prescription, compares with other class sample solution to possess following characteristics: to corresponding nurseries and kindergartens mechanism surface disinfection liquid have solid in and sampling action; Neutralizing agent itself or with the product of nurseries and kindergartens mechanism body surface sample solution reaction the experiment microorganism is not had killing or suppress the effect of its growth; Do not have destruction to cultivating composition, also do not influence its physical behavior.
Summary of the invention main points of the present invention are to select suitable component, and rationally are mixed, through heat, dissolve, technology such as mixing, cooling, packing, high-temperature sterilization forms.
The prescription following (component and weight percent % thereof) that the present invention selects: glycine 1.0-3.0; Tween-80 1.0-3.0; Sulfothiorine 1.0-3.0; Histidine 1.0-3.0; Nutrient broth 1.0-2.0; Phosphate buffered water adds to 100.
Glycine, tween-80, Histidine, Sulfothiorine, meat soup can with thimerosal generation chemical reaction in the nurseries and kindergartens body surface of remnants, generate to the experiment microorganism do not kill or suppress its growth effect, do not have destruction to cultivating composition, physical behavior not have the neutralized reaction product that influences.Corresponding medical object surface thimerosal had solid neutralizing effect.
The preparation method of product of the present invention is:
1), tween-80, nutrient broth are heated in proportion, is dissolved in the phosphate buffered water;
2), glycine, Histidine, Sulfothiorine are dissolved in the phosphate buffered water in proportion;
3), will be above-mentioned two liquid mix, carry out packing after the cooling, 105 ℃ of sterilizations 15 minutes, check, qualified back is standby.
The present invention has the following advantages: corresponding nurseries and kindergartens surface disinfection liquid is had solid neutralizing effect; Neutralizing agent itself or with nurseries and kindergartens mechanism body surface in and the product of sample solution reaction the experiment microorganism is not had killing or suppresses the effect of its growth; Do not have destruction to cultivating composition, also do not influence its physical behavior.
The present invention is described in further detail below in conjunction with embodiment for embodiment:
Be configured according to following table institute column data (weight percent %) and described step thereof:
Claims (2)
1. the present invention relates to a kind of nurseries and kindergartens mechanism sterilisation effect supervision sampling neutralizer, it is characterized in that having following prescription (component and weight percent % thereof): glycine 1.0-3.0; Tween-80 1.0-3.0; Sulfothiorine 1.0-3.0; Histidine 1.0-3.0; Nutrient broth 1.0-2.0; Phosphate buffered water adds to 100.
2. the described nurseries and kindergartens of claim 1 a mechanism sterilisation effect is supervised the sampling neutralizer, it is characterized in that having following preparation technology:
1), tween-80, nutrient broth are heated in proportion, is dissolved in the phosphate buffered water;
2), glycine, Histidine, Sulfothiorine are dissolved in the phosphate buffered water in proportion;
3), will be above-mentioned two liquid mix, carry out packing after the cooling, 105 ℃ of sterilizations 15 minutes, check, qualified back is standby.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2012100016299A CN103194526A (en) | 2012-01-05 | 2012-01-05 | Childcare institution disinfection effect supervision and sampling neutralizing liquid |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2012100016299A CN103194526A (en) | 2012-01-05 | 2012-01-05 | Childcare institution disinfection effect supervision and sampling neutralizing liquid |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN103194526A true CN103194526A (en) | 2013-07-10 |
Family
ID=48717341
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2012100016299A Pending CN103194526A (en) | 2012-01-05 | 2012-01-05 | Childcare institution disinfection effect supervision and sampling neutralizing liquid |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103194526A (en) |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101463385A (en) * | 2007-12-18 | 2009-06-24 | 曲奕 | Medical institution personnel hand neutralization sampling solution |
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2012
- 2012-01-05 CN CN2012100016299A patent/CN103194526A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101463385A (en) * | 2007-12-18 | 2009-06-24 | 曲奕 | Medical institution personnel hand neutralization sampling solution |
Non-Patent Citations (2)
| Title |
|---|
| 林凤: "不同中和剂对几种消毒剂的试验效果观察", 《预防医学情报杂志》 * |
| 陈淑敏: "《医院感染预防与控制临床指南》", 31 August 2011 * |
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| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C05 | Deemed withdrawal (patent law before 1993) | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20130710 |