CN103194525A - Novel bio-chemical public hygiene gas sterilization effect indicating agent - Google Patents
Novel bio-chemical public hygiene gas sterilization effect indicating agent Download PDFInfo
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- CN103194525A CN103194525A CN2012100016388A CN201210001638A CN103194525A CN 103194525 A CN103194525 A CN 103194525A CN 2012100016388 A CN2012100016388 A CN 2012100016388A CN 201210001638 A CN201210001638 A CN 201210001638A CN 103194525 A CN103194525 A CN 103194525A
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- sterilizing
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Abstract
The invention relates to a novel bio-chemical public hygiene gas sterilization effect indicating agent, and is a special identification reagent used for detecting the effect of a polluted public environment sterilized by using a sterilizing gas. The indicating agent can be used in supervision and inspection of the fields of public hygiene, technical supervision and the like, and is used for identifying disinfection and sterilizing effects of ethylene oxide, glutaraldehyde, formaldehyde, and ozone. The indicating agent provided by the invention has the advantages that: 1, the novel bio-chemical public hygiene gas sterilization effect indicating agent is suitable for sterilizing gas effect detections in various occasions; 2, no special culturing condition is need, and cost and price are low; 3, operation method is simple and feasible, a rapid one-step method is achieved in the detection step, and detection time is short; 4, the product has good sensitivity, high specificity, and high accuracy; 5, sterilizing gas sterilizing degree can be detected, gas quantitative analysis can be carried out, and disinfection time can be indicated rapidly and accurately; and 6, the public hygiene gas sterilization effect indicating agent is placed into a plastic syringe-like device, such that the indicating agent is not affected by interfering factors in detections.
Description
Technical field the present invention relates to a kind of new bio-chemical public health gaseous sterilization effect indicator, is that a kind of being specifically designed to detected contaminated PE, with the indentifying substance of the effect after the sterilizing gas sterilization.Its main ingredient is the device of magnesium chloride solution, tetrabromophenol sulfonphthalein hydrochloric acid soln, bacillus subtilis black variety gemma bacterium liquid, neutral filter paper, TGA salt culture medium, plastic injector sample.Through paper-cut, sterilization, dipping, dry, increase bacterium cultivate, drip dye, dry, technology such as sterile packed forms a kind of professional new bio-chemical public health gaseous sterilization effect indicator.Can be used for field supervision such as public health, technical supervision and detect, differentiate oxyethane, glutaraldehyde, formaldehyde, the sterilization of ozone, sterilising effect.Existing sterilization gas detection reagent kind is a lot, and performance differs, and susceptibility is poor, specificity is weak, accuracy is low; Need the specific culture condition; Cost and price height; The working method complexity detects step and does not reach quick single stage method, and detection time is long; The detected result of sterilization gas can't quantitative analysis; In detection, be subject to shortcomings such as interfering factors influence.Therefore, every profession and trade wishes to have a kind of new bio-chemical public health gaseous sterilization effect indicator that can overcome the existing procucts deficiency always.
Sterilization gas such as background technology oxyethane, glutaraldehyde, formaldehyde, ozone be a kind of spectrum, efficient, penetration power strong, to slight sterilization, the disinfectant of sterilizing objects infringement.At home and abroad, especially developed country is extensive use of the mixing sterilization gas of oxyethane, glutaraldehyde, formaldehyde to the sterilization of medicine equipment, various fabric, plastics, fur article, public place etc.The quality of sterilising effect directly reflects the quality that is sterilized product.Check the effect of the mixing sterilization gas sterilization of oxyethane, glutaraldehyde, formaldehyde with several test-papers, check that qualitatively sterilization bag and other sterilized containers have or not the mixing sterilization gas gas that spills oxyethane, glutaraldehyde, formaldehyde, ozone, thereby infer sterilisation effect indirectly, by domestic and international widespread use.The object of the present invention is to provide a kind of existing procucts deficiency that can overcome, the two-way indication new bio of biological-chemical indicator-chemical public health gaseous sterilization effect indicator.Having single sterilising effect of planting, mixing sterilization gas that can be suitable for oxyethane, glutaraldehyde, formaldehyde, ozone detects; Do not need the specific culture condition; Cost and price are low; Working method is simple and easy to do, detects step and reaches quick single stage method, and detection time is short; Susceptibility is good, high specificity, accuracy height; Single sterilising effect of planting, mixing sterilization gas of oxyethane, glutaraldehyde, formaldehyde, ozone detects; Can not only detect the sterilization degree, can also plant, mix the gasometric analysis of sterilization gas the list of oxyethane, glutaraldehyde, formaldehyde, ozone, and can represent disinfecting time; Characteristics such as interference-free factor affecting in detection.
Summary of the invention main points of the present invention are to select suitable component, and rationally prepare, through paper-cut, sterilization, dipping, dry, increase bacterium cultivate, drip dye, dry, new bio-chemical public health gaseous sterilization effect indicator that technology such as sterile packed forms a kind of specialty.
The prescription that the present invention selects is as follows: 5*10
6/ ml bacillus subtilis black variety gemma bacterium liquid 0.06-0.08ml; TGA salt culture medium 16-18ml; Magnesium chloride solution 100-150%; Tetrabromophenol sulfonphthalein hydrochloric acid soln 4-5%; Neutral filter paper 0.6 * 2.5cm; The device 10-20ml of plastic injector sample.
With oxyethane, glutaraldehyde, formaldehyde gas reaction, generated magnesium hydroxide after filter paper steeps in saturated magnesium chloride solution, become alkalescence PH is raise, sterilization gas concentration is more high, and duration of contact is more of a specified duration, and color relation is more dark.Indicating card is placed in the sterilizing objects position of difficult sterilization, with together disinfection of article.The paper variable color situation of inspecting is soon observed in the sterilization back.The color of inspecting paper is deepened gradually with the dosage of contact sterilization gas and the increase of time, soon: by original white or light blue, gradually become light blue-mazarine-blue-greenish colour-deep green.Generally reach blue-greenish colour and deep green person, the indication sterilising effect is good.Dying on the indicating card has bacillus subtilis black variety gemma bacterium liquid, can drop into after the sterilization in the liquid hydrogen sulfenyl acetate substratum, cultivates 7 days down in 60 ℃, observes to have or not growth.In order to make indicating card can indicate disinfecting time, put in the device of plastic injector sample inspecting paper soon, put into the unapproachable position of sterilization gas.Sterilization gas is penetrated into to be inspected paper soon and needs 1.5 hours approximately, by observing the variable color situation of inspecting paper soon, not only show contact sterilization gas what, also signable disinfecting time.
The preparation method of product of the present invention is:
1), filter paper is cut into 0.5 * 2cm size, interior 10 minutes of immersion 100-150% magnesium chloride solution, 37 ℃ of oven dry;
2), more above-mentioned filter paper bar was immersed in the 4-5% tetrabromophenol sulfonphthalein hydrochloric acid soln airing 10 minutes;
3), indicator bacillus subtilis black variety gemma bacterium liquid cultivates 48h, is prepared as 5*10
6The bacteria suspension of/ml drips and dyes 0.02ml;
4), make that indicating card dries, sterile packed.
The present invention has the following advantages:
1. new bio-chemical public health gaseous sterilization effect indicator is suitable for the detection of the sterilizing gas effect of various occasions.
2. do not need the specific culture condition, cost and cheap.3. working method is simple and easy to do, detects step and reaches quick single stage method, and detection time is short.4. good, the high specificity of the susceptibility of product, accuracy height.5. can not only detect the sterilization degree of sterilization gas, can also be to gasometric analysis, and can indicate disinfecting time fast and accurately.6. public health gaseous sterilization effect indicator is put in the device of plastic injector sample, interference-free factor affecting in detection.
The present invention is described in further detail below in conjunction with embodiment for embodiment:
Be prepared according to following table institute's column data and described step thereof:
5*10
6/ ml bacillus subtilis black variety gemma bacterium liquid 0.06-0.08ml; TGA salt culture medium 16-18ml; Magnesium chloride solution 100-150%; Tetrabromophenol sulfonphthalein hydrochloric acid soln 4-5%; Neutral filter paper 0.6 * 2.5cm; The device 10-20ml of plastic injector sample.
1), filter paper is cut into 0.5 * 2cm size, interior 10 minutes of immersion 100-150% magnesium chloride solution, 37 ℃ of oven dry;
2), more above-mentioned filter paper bar was immersed in the 4-5% tetrabromophenol sulfonphthalein hydrochloric acid soln airing 10 minutes;
3), indicator bacillus subtilis black variety gemma bacterium cultivates 48h, is prepared as 5*10
6The bacteria suspension of/ml drips and dyes 0.02ml;
4), make that indicating card dries, sterile packed.
Claims (2)
1. the present invention relates to a kind of new bio-chemical public health gaseous sterilization effect indicator, it is characterized in that having following prescription: 5*10
6/ ml bacillus subtilis black variety gemma bacterium liquid 0.06-0.08ml; TGA salt culture medium 16-18ml; Magnesium chloride solution 100-150%; Tetrabromophenol sulfonphthalein hydrochloric acid soln 4-5%; Neutral filter paper 0.6 * 2.5cm; The plastic injector sampling device of 10-20ml.
2. the described a kind of new bio of claim 1-chemical public health gaseous sterilization effect indicator is characterized in that having following preparation technology:
1), filter paper is cut into 0.5 * 2cm size, interior 10 minutes of immersion 100-150% magnesium chloride solution, 37 ℃ of oven dry;
2), more above-mentioned filter paper bar was immersed in the 4-5% tetrabromophenol sulfonphthalein hydrochloric acid soln airing 10 minutes;
3), indicator bacillus subtilis black variety gemma bacterium liquid cultivates 48h, is prepared as the bacteria suspension of 5*106/ml, drip and dye 0.02ml;
4), make that indicating card dries, sterile packed.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2012100016388A CN103194525A (en) | 2012-01-05 | 2012-01-05 | Novel bio-chemical public hygiene gas sterilization effect indicating agent |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2012100016388A CN103194525A (en) | 2012-01-05 | 2012-01-05 | Novel bio-chemical public hygiene gas sterilization effect indicating agent |
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| CN103194525A true CN103194525A (en) | 2013-07-10 |
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| CN2012100016388A Pending CN103194525A (en) | 2012-01-05 | 2012-01-05 | Novel bio-chemical public hygiene gas sterilization effect indicating agent |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112724964A (en) * | 2020-12-29 | 2021-04-30 | 优品保技术（北京）有限公司 | Disinfectant with indication and tracing functions and application thereof |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101541944A (en) * | 2006-09-20 | 2009-09-23 | 美国杀菌器公司 | Sterilization indicator |
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2012
- 2012-01-05 CN CN2012100016388A patent/CN103194525A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101541944A (en) * | 2006-09-20 | 2009-09-23 | 美国杀菌器公司 | Sterilization indicator |
Non-Patent Citations (2)
| Title |
|---|
| 张晓春等: "正确使用环氧乙烷灭菌效果的监测方法", 《中华医院感染学杂志》 * |
| 杨革: "《微生物学实验教程》", 31 January 2010 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112724964A (en) * | 2020-12-29 | 2021-04-30 | 优品保技术（北京）有限公司 | Disinfectant with indication and tracing functions and application thereof |
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Application publication date: 20130710 |