CN104749175A - Method for detecting organophosphorus pesticide residues in fruits and vegetables - Google Patents
Method for detecting organophosphorus pesticide residues in fruits and vegetables Download PDFInfo
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- CN104749175A CN104749175A CN201510148324.4A CN201510148324A CN104749175A CN 104749175 A CN104749175 A CN 104749175A CN 201510148324 A CN201510148324 A CN 201510148324A CN 104749175 A CN104749175 A CN 104749175A
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Abstract
The invention discloses a method for detecting organophosphorus pesticide residues in fruits and vegetables, and belongs to the field of food safety detection. The method comprises the following steps: soaking blank filter paper in a solution to be detected for 1min, airing, fumigating with bromine vapour, then developing by taking 5% Congo ethanol solution as a developing reagent, and directly reading the content of organophosphorus pesticides in the solution to be detected by comparing with colours on a standard colourimetric card. The invention provides a rapid detection method for organophosphorus pesticide contents in fruits and vegetables, wherein the detectable pesticide concentration range is 0-100mg/L. The method provided by the invention has the advantages of being sensitive, rapid, simple and convenient to operate, low in cost, and the like, does not need to be operated by professionals, facilitates self-use of common people in families, and can also be used for rapid detection in industrial and commercial law enforcement, food safety and markets.
Description
Technical field
The invention belongs to field of detection of food safety, particularly a kind of method detecting organophosphorus pesticide residue in fruit and vegetable.
Background technology
World today people tendency be the food of green health, the fresh fruit of vegetables of low pesticide concentration has caused the strong interest of various circles of society, and progressively becomes the development trend of green health food.In recent years, the unreasonable use of agricultural chemicals and excessively using, the Pesticide Residue in the agricultural product such as fruit and vegetable has become the heat that Chinese society shows great attention to and has determined problem.
Organophosphorus insecticide is widely used in desinsection, the sterilization and weeding etc. of crops, it is the maximum class agricultural chemicals of China's use amount, it belongs to neurotoxic substance, the activity of enzyme acetylcholine in main suppression blood and tissue, cause neurotransmitter, a large amount of savings of acetylcholine, thus blocked spiritual conduction, thus cause nervous centralis poisoning.The pesticide species of strictly registering in China has reached 363 kinds, and the subject matter that Pesticide use exists is that Irrational Use of Drugs and high-toxic pesticide are still in a large amount of use.In order to ensure that consumers in general's is healthy, research measures Pesticide Residue in Vegetable mensuration fast, comprehensively and has very important significance.
The detection method of current organophosphorus pesticide is numerous, generally has spotting method, photometry, liquid phase chromatography and vapor-phase chromatography etc. on inhibiting AChE, immunization, paper.Above-mentioned detection method all could need operate under the certain condition environment such as laboratory, and the process in early stage of the method such as liquid phase, gas phase sample is more complicated, and the operation of large-scale instrument need carry out special training, and testing cost is high, and the time is long.Therefore, be badly in need of a kind of cost low, use simply and fast method for detecting the content of Pesticide Residue in Vegetable.
Summary of the invention
The object of the invention is to overcome the shortcoming that exists in prior art with not enough, a kind of method detecting organophosphorus pesticide residue in fruit and vegetable is provided.
Object of the present invention is achieved through the following technical solutions: a kind of method detecting organophosphorus pesticide residue in fruit and vegetable, comprises the steps:
Blank filter paper is placed in solution to be measured and soaks 1min, dry, after bromine vapor is stifling, develop the color as chromogenic reagent using 5% the Congo's ethanolic solution, by with the color contrast on standard color comparison card, the directly content of organophosphorus pesticide in reading solution to be measured.
Described blank filter paper is the filter paper of quantitative filter paper, qualitative filter paper or other materials, is preferably qualitative filter paper; The selection of filter paper is can adsorb to be tested and chromogenic reagent for standard;
The size of described blank filter paper is 3cm × 0.5cm.
Described solution to be measured is adopted and is prepared with the following method: fruit and vegetable 10% aqueous sodium persulfate solution is soaked 10min, obtains solution to be measured.
Organophosphorus pesticide concentration corresponding in described standard color comparison card is respectively 0mg/L, 20mg/L, 40mg/L, 60mg/L, 80mg/L and 100mg/L.
Described standard color comparison card is adopted and is prepared with the following method:
1) draw respectively 200mg/L organophosphorus standard solution 0,1,2,3,4,5mL, join in 6 10mL color comparison tubes, distilled water is settled to scale;
2) blank for 6 3cm × 0.5cm qualitative filter papers are immersed in step 1 respectively completely) color comparison tube in, after 1min, taking-up is dried;
3) by step 2) filter paper that dries be placed in stifling on bromine vapor after, an each upper 5% Congo red ethanolic solution, after chromogenic reaction terminates, by the color on each test-paper of cameras record, colour print obtains the standard color comparison card of organophosphorus pesticide.
Obtained organophosphorus pesticide standard color comparison card carries out plastic packaging, prevents contaminated or damages.
In described detection fruit and vegetable, the method for organophosphorus pesticide residue can be applied to the concentration range detecting organophosphorus pesticide is 0 ~ 100mg/L.
The present invention has following advantage and effect relative to prior art:
(1) the invention provides a kind of method for quick for organophosphorus pesticide content in fruit and vegetable, the pesticide concentration scope that can detect is 0 ~ 100mg/L.
(2) method of the present invention has sensitive, quick, easy and simple to handle and low cost and other advantages, and without the need to professional's operation, is convenient to the common people and uses voluntarily in the family, also can be used for the quick detection in industrial and commercial law enforcement, food security and market.
Accompanying drawing explanation
Fig. 1 is the other colour atla of organophosphorus pesticide standard of embodiment 3.
Fig. 2 is the testing result figure of organophosphorus pesticide content in 4 kinds of fruit and vegetables of embodiment 4.
Embodiment
Below in conjunction with embodiment and accompanying drawing, the present invention is described in further detail, but embodiments of the present invention are not limited thereto.
Embodiment 1
Get 3 parts not containing each 10mL of blank distilled water of organophosphorus pesticide, the blank qualitative filter paper sheet respectively 3 being of a size of 3cm × 0.5cm is immersed in each solution completely, take out after 1min and dry, be placed in after bromine vapor is fumigated, instill 1 5% Congo red ethanolic solution, question response terminates rear observation color result of variations.Result shows, 3 containing the test-paper color result indifference of agricultural chemicals.
Embodiment 2
Getting 3 parts containing organophosphorus pesticide concentration is each 10mL of 20mg/L standard aqueous solution, the blank qualitative filter paper sheet respectively 3 being of a size of 3cm × 0.5cm is immersed in each solution completely, take out after 1min and dry, be placed in after bromine vapor is fumigated, instill 1 5% Congo red ethanolic solution, question response terminates rear observation color result of variations.Result shows, 3, containing the test-paper color result indifference of organophosphorus pesticide, illustrate that the method is reproducible.
Embodiment 3
Draw organophosphorus pesticide titer 0 that concentration is 200mg/L, 1,2,3,4,5mL is added to distilled water constant volume in 6 10ml color comparison tubes respectively.The blank qualitative filter paper sheet respectively 6 being of a size of 3cm × 0.5cm is immersed in each solution completely, and take out after 1min and dry, be placed in after bromine vapor is fumigated, instill 1 5% Congo red ethanolic solution, question response terminates rear observation color result of variations.Color result color printing plastic packaging is preserved, makes and become standard color comparison card, see Fig. 1.
Embodiment 4
The present embodiment adopts the standard color comparison card of organophosphorus pesticide in detection method in embodiment 1,2 and embodiment 3.Get 2 strawberries, 2 little tomatoes, 3 French beans and 1 cuke, weigh respectively and record lifting capacity, being placed in 4 clean beakers of 100mL, numbering 1,2,3,4, add 10% aqueous sodium persulfate solution 50mL, take out fruits and vegetables after concussion drip washing 5min, obtain liquid to be measured.
Getting 4 sizes is that the blank filter paper of 3cm × 0.5cm is immersed in 4 organophosphorus pesticide liquid to be measured, take out after 1min and dry, after fumigating with bromine vapor again, each instillation 1 5% Congo red ethanolic solution, after color reaction terminates, organophosphorus pesticide standard color comparison card in test-paper and embodiment 3 is contrasted, directly reads the content of organophosphorus pesticide in liquid to be measured.Replicate determination three times, Fig. 2 is shown in color change, and testing result data are in table 1.
Table 1 testing result
Found by contrast test, the time of fruit and vegetable being soaked drip washing is increased to 30min or longer time, measured organophosphorus pesticide content results and immersion 5min indifference.
Above-described embodiment is the present invention's preferably embodiment; but embodiments of the present invention are not restricted to the described embodiments; change, the modification done under other any does not deviate from Spirit Essence of the present invention and principle, substitute, combine, simplify; all should be the substitute mode of equivalence, be included within protection scope of the present invention.
Claims (8)
1. detect a method for organophosphorus pesticide residue in fruit and vegetable, it is characterized in that, comprise the steps:
Blank filter paper is placed in solution to be measured and soaks 1min, dry, after bromine vapor is stifling, develop the color as chromogenic reagent using 5% the Congo's ethanolic solution, by with the color contrast on standard color comparison card, the directly content of organophosphorus pesticide in reading solution to be measured.
2. the method detecting organophosphorus pesticide residue in fruit and vegetable according to claim 1, it is characterized in that, the size of described blank filter paper is 3cm × 0.5cm.
3. the method detecting organophosphorus pesticide residue in fruit and vegetable according to claim 1, it is characterized in that, described blank filter paper is the filter paper of quantitative filter paper, qualitative filter paper or other materials.
4. the method detecting organophosphorus pesticide residue in fruit and vegetable according to claim 3, it is characterized in that, described blank filter paper is qualitative filter paper.
5. the method detecting organophosphorus pesticide residue in fruit and vegetable according to claim 1, is characterized in that, described solution to be measured is adopted and prepared with the following method: fruit and vegetable 10% aqueous sodium persulfate solution is soaked 10min, obtains solution to be measured.
6. the method detecting organophosphorus pesticide residue in fruit and vegetable according to claim 1, it is characterized in that, organophosphorus pesticide concentration corresponding in described standard color comparison card is respectively 0mg/L, 20mg/L, 40mg/L, 60mg/L, 80mg/L and 100mg/L.
7. the method detecting organophosphorus pesticide residue in fruit and vegetable according to claim 1, it is characterized in that, described standard color comparison card is adopted and is prepared with the following method:
1) draw respectively 200mg/L organophosphorus standard solution 0,1,2,3,4,5mL, join in 6 10mL color comparison tubes, distilled water is settled to scale;
2) blank for 6 3cm × 0.5cm qualitative filter papers are immersed in step 1 respectively completely) color comparison tube in, after 1min, taking-up is dried;
3) by step 2) filter paper that dries be placed in stifling on bromine vapor after, an each upper 5% Congo red ethanolic solution, after chromogenic reaction terminates, by the color on each test-paper of cameras record, colour print obtains the standard color comparison card of organophosphorus pesticide.
8. in detection fruit and vegetable according to claim 1, the method for organophosphorus pesticide residue is applied to the concentration range detecting organophosphorus pesticide is 0 ~ 100mg/L.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201510148324.4A CN104749175A (en) | 2015-03-31 | 2015-03-31 | Method for detecting organophosphorus pesticide residues in fruits and vegetables |
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| CN201510148324.4A CN104749175A (en) | 2015-03-31 | 2015-03-31 | Method for detecting organophosphorus pesticide residues in fruits and vegetables |
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| CN201510148324.4A Pending CN104749175A (en) | 2015-03-31 | 2015-03-31 | Method for detecting organophosphorus pesticide residues in fruits and vegetables |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105021604A (en) * | 2015-07-15 | 2015-11-04 | 江苏大学 | Fast imaging detection method of heavy metal element content and device thereof |
| CN105938102A (en) * | 2016-07-15 | 2016-09-14 | 四川理工学院 | Method for rapid determination of pesticide residues in fruits and vegetables through chemical developing method |
| CN109655452A (en) * | 2017-10-11 | 2019-04-19 | 中国科学院大连化学物理研究所 | A method of organophosphorus pesticide in the colorimetric detection fruits and vegetables based on filter paper |
| CN111323413A (en) * | 2020-02-17 | 2020-06-23 | 中国农业大学 | Device for detecting pesticide by evaporating solution-nucleic acid test paper |
| CN112378904A (en) * | 2020-11-24 | 2021-02-19 | 临沂华澳食品有限公司 | Rapid detection method for pesticide residues in melons, fruits and vegetables |
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| CN201660632U (en) * | 2009-10-16 | 2010-12-01 | 北京智云达科技有限公司 | Quick fruit and vegetable pesticide residue testing card |
| CN103728298A (en) * | 2014-01-28 | 2014-04-16 | 黄石市食品药品检验所 | Quick quantity-limited detection method for detecting quantity of sulfur dioxide residual in traditional Chinese medicinal materials, decoction pieces of traditional Chinese medicinal materials, agricultural products and foods |
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| CN201660632U (en) * | 2009-10-16 | 2010-12-01 | 北京智云达科技有限公司 | Quick fruit and vegetable pesticide residue testing card |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105021604A (en) * | 2015-07-15 | 2015-11-04 | 江苏大学 | Fast imaging detection method of heavy metal element content and device thereof |
| CN105938102A (en) * | 2016-07-15 | 2016-09-14 | 四川理工学院 | Method for rapid determination of pesticide residues in fruits and vegetables through chemical developing method |
| CN105938102B (en) * | 2016-07-15 | 2020-01-14 | 四川理工学院 | Method for rapidly determining pesticide residues in fruits and vegetables by chemical color development method |
| CN109655452A (en) * | 2017-10-11 | 2019-04-19 | 中国科学院大连化学物理研究所 | A method of organophosphorus pesticide in the colorimetric detection fruits and vegetables based on filter paper |
| CN111323413A (en) * | 2020-02-17 | 2020-06-23 | 中国农业大学 | Device for detecting pesticide by evaporating solution-nucleic acid test paper |
| CN112378904A (en) * | 2020-11-24 | 2021-02-19 | 临沂华澳食品有限公司 | Rapid detection method for pesticide residues in melons, fruits and vegetables |
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