CN101463383A - Sterilizing gas rapid detection reagent label - Google Patents

Abstract

The invention relates to a sterilization gas fast detection reagent paste. The main components comprise sodium carbonate, 4-[4-nitrobenzyl] pyridine, bacillus stearothemophilus liquid, 0.6*2.5cm neutral filter paper, a 10-15ml sulphydryl acetate culture medium and a 10-20ml plastic injector type device. The sterilization gas fast detection reagent paste is a special biological-chemical indicator and a novel sterilization gas fast detection reagent paste which is manufactured by the technologies of paper-cutting, sterilizing, dipping, drying, enriched culturing, drop dyeing, airing, aseptic packaging and the like and can be used as an identification reagent for monitoring and detecting the epoxy ethane sterilization effect in the fields such as medical treatment, sanitation, epidemic prevention, scientific research, teaching, environmental protection, food, animal husbandry, aquatic product, biological engineering, industry and commerce, quarantine, laundry and engineering supervision, etc. The sterilization gas fast detection reagent paste has the advantages as follows: the sterilization gas fast detection reagent paste is applicable to the detection of the effect of sterilization gas in various industries; the sterilization gas fast detection reagent paste is simple, fast and easy for operating and does not require special culture condition; the sterilization gas fast detection reagent paste has low cost and price; the sterilization gas fast detection reagent paste has good sensitivity, strong specificity and high accuracy; the sterilization gas fast detection reagent paste not only can detect the sterilization degree of the epoxy ethane but also can carry out the gas quantitative analysis of the epoxy ethane and can mark sterilization time fast and exactly to ensure that the detection step realizes a fast one-step method; and the sterilization gas fast detection reagent paste is not affected by interference factors.

Description

Sterilizing gas rapid detection reagent label

Technical field the present invention relates to a kind of sterilizing gas rapid detection reagent label, is a kind of indentifying substance that is specifically designed to the sterilising effect of rapid detection ethylene oxide sterilizing gas, and indicator is a bacillus stearothermophilus.Its main ingredient is yellow soda ash, 4-[4-nitrobenzyl] pyridine, bacillus stearothermophilus fat thermophilic bacteria sporeformer liquid; 0.6 the neutral filter paper of * 2.5cm; 10-15ml TGA salt culture medium; The device of 10-20ml plastic injector sample.Through paper-cut, sterilization, dipping, dry, increase bacterium cultivate, drip dye, dry, biology-chemical indicator that technology such as sterile packed forms a kind of specialty, the fast check reagent of novel sterilizing gas pastes.The fast check reagent of oxyethane.The indentifying substance that can be used for every field supervision detection ring oxidative ethane sterilisation effects such as medical treatment, health, epidemic prevention, scientific research, teaching, environmental protection, food, herding, aquatic products, biotechnology, industry and commerce, quarantine, laundry, technical supervision.Existing oxyethane detection reagent kind is a lot, and performance differs, and susceptibility is poor, specificity is weak, accuracy is low; Need the specific culture condition; Cost and price height; The working method complexity detects step and does not reach quick single stage method, and detection time is long; The detected result of oxyethane can't quantitative analysis; In detection, be subject to shortcomings such as interfering factors influence.Therefore, every profession and trade is wished to have always and a kind ofly can be overcome the insufficient sterilizing gas rapid detection reagent label of existing procucts.

Background technology oxyethane mix sterilization gas be a kind of spectrum, efficient, penetration power is strong, to slight sterilization, the disinfectant of sterilizing objects infringement.At home and abroad, especially developed country is extensive use of the sterilization of oxyethane to medicine equipment, various fabric, plastics, fur article etc.The quality of sterilising effect directly reflects the quality that is sterilized product.Check the effect of oxirane disinfections with several test-papers, or check qualitatively that sterilization bag and other sterilized containers have or not and spill ethylene oxide gas, thereby infer sterilisation effect indirectly, by domestic and international widespread use.

The object of the present invention is to provide that a kind of to overcome existing procucts insufficient, biology-chemical indicator, the fast check reagent of novel sterilizing gas pastes.Sterilising effect with the oxyethane that can be suitable for various occasions detects; Do not need the specific culture condition; Cost and price are low; Working method is simple and easy to do, detects step and reaches quick single stage method, and detection time is short; Susceptibility is good, high specificity, accuracy height; The sterilising effect of oxyethane detects can not only detect the sterilization degree, can also be to the gasometric analysis of oxyethane, and can represent disinfecting time; Characteristics such as interference-free factor affecting in detection.

Summary of the invention main points of the present invention are to select suitable component, and rationally prepare, through paper-cut, sterilization, dipping, dry, increase bacterium cultivate, drip dye, dry, biology-chemical indicator that technology such as sterile packed forms a kind of specialty, the fast check reagent of novel sterilizing gas pastes.

The prescription that the present invention selects is as follows: the 1-3% sodium carbonate solution; 1-3%4-[4-nitrobenzyl] pyridine acetone soln; 0.02-0.05ml106 fatty thermophilic bacteria sporeformer liquid; 0.6 the neutral filter paper of * 2.5cm; 10-15ml TGA salt culture medium; The device of 10-20ml plastic injector sample.

After steeping, filter paper becomes alkalescence in sodium carbonate solution, oxyethane and 4-[4-nitrobenzyl] pyridine issues biochemical reaction at alkaline condition, the intermediate product dehydration back that generates forms methine dyes, ethylene oxide concentration is high more, duration of contact is of a specified duration more, the methine dyes that generates is many more, and color relation is dark more.

To inspect paper soon and be placed on the most difficult disinfectant position in the sterilizing objects, with together disinfection of article.The paper variable color situation of inspecting is soon observed in the sterilization back.The color of inspecting paper is deepened gradually with the dosage of contactless ring oxidative ethane and the increase of time, soon: by original white or faint yellow, gradually become light blue-mazarine-black-and-blue-grey black.Generally reach black-and-blue and the grey black person, the indication sterilising effect is good.Inspecting soon to dye on the paper has fatty thermophilic bacteria gemma, can drop into after the sterilization in the liquid hydrogen sulfenyl acetate substratum, cultivates 7 days down in 60 ℃, observes to have or not growth.To inspect paper soon and can indicate disinfecting time in order to make, put in the device of plastic injector sample, put into the unapproachable position of oxyethane inspecting paper soon.Oxyethane is penetrated into to be inspected paper soon and needs 1.5 hours approximately, by observing the variable color situation of inspecting paper soon, not only show the contactless ring oxidative ethane what, also signable disinfecting time.

The preparation method of product of the present invention is:

1), filter paper is cut into 0.5 * 2cm size, immersed in 2% sodium carbonate solution airing or 37 ℃ of oven dry 10 minutes;

2), more above-mentioned filter paper bar was immersed in 2%4-[4-nitrobenzyl] the pyridine acetone solns airing 10 minutes;

3), indicator fat thermophilic bacteria gemma cultivates 48h, is prepared as the bacteria suspension of 5*106, drips and dyes 0.02ml, make to inspect soon that paper dries, sterile packed.

The present invention has the following advantages: (1). and sterilizing gas rapid detection reagent label is suitable for the detection of the ethylene oxide sterilizing effect of various occasions.(2). do not need the specific culture condition.(3). cost and cheap.(4). detect step and reach quick single stage method, detection time is short.(5). the susceptibility of product is good, high specificity, accuracy height.(6). working method is simple and easy to do, makes advanced Measurement for Biotechnique be simplified to general personnel and can both grasp operation.(7). inspect the sterilization degree that paper can not only the detection ring oxidative ethane soon, can also be to the gasometric analysis of oxyethane, and can indicate disinfecting time fast and accurately; (8). sterilizing gas rapid detection reagent label is put in the device of plastic injector sample, interference-free factor affecting in detection.

The present invention is described in further detail below in conjunction with embodiment for embodiment:

Be prepared according to following table institute's column data and described step thereof:

Prescription one:

The 1-3% sodium carbonate solution;

1-3%4-[4-nitrobenzyl] pyridine acetone soln;

0.02-0.05ml106 fatty thermophilic bacteria sporeformer liquid;

0.6 the neutral filter paper of * 2.5cm;

10-15ml TGA salt culture medium;

The device of 10-20ml plastic injector sample.

Prescription two:

The 1-2.5% sodium carbonate solution;

1-2.5%4-[4-nitrobenzyl] pyridine acetone soln;

0.02-0.04ml106 fatty thermophilic bacteria sporeformer liquid;

0.6 the neutral filter paper of * 2.0cm;

10-13ml TGA salt culture medium;

The device of 10-25ml plastic injector sample.

Prescription three:

The 1.5-3% sodium carbonate solution;

1.5-3%4-[4-nitrobenzyl] pyridine acetone soln;

0.03-0.05ml106 fatty thermophilic bacteria sporeformer liquid;

0.8 the neutral filter paper of * 2.5cm;

12-15ml TGA salt culture medium;

The device of 15-20ml plastic injector sample.

Prescription four:

The 1.5-2.5% sodium carbonate solution;

1.5-2.5%4-[4-nitrobenzyl] pyridine acetone soln;

0.03-0.04ml106 fatty thermophilic bacteria sporeformer liquid;

0.8 the neutral filter paper of * 2.0cm;

12-14ml TGA salt culture medium;

The device of 15-18ml plastic injector sample.

Claims (2)

1, sterilizing gas rapid detection reagent label is with sodium carbonate solution, 4-[4-nitrobenzyl] device of pyridine solution, bacillus stearothermophilus bacterium liquid, neutral filter paper, TGA salt culture medium, plastic injector sample through a series of paper-cuts, sterilization, dipping, dry, increase bacterium cultivate, drip dye, dry, technology such as sterile packed forms; It is characterized in that having following prescription: sodium carbonate solution 1-3%; 4-[4-nitrobenzyl] pyridine acetone soln 1-3%; 106 fatty thermophilic bacteria sporeformer liquid 0.02-0.05ml; Neutral filter paper 0.6 * 2.5cm; TGA salt culture medium 10-15ml; The device of 10-20ml plastic injector sample.

2, the preparation method of the described sterilizing gas rapid detection reagent label of a kind of claim 1 is characterized in that having following step:

1), filter paper is cut into 0.5 * 2cm size, immersed in 2% sodium carbonate solution airing or 37 ℃ of oven dry 10 minutes;

2), more above-mentioned filter paper bar was immersed in 2%4-[4-nitrobenzyl] the pyridine acetone solns airing 10 minutes;

3), indicator fat thermophilic bacteria gemma cultivates 48h, is prepared as the bacteria suspension of 5*106, drips and dyes 0.02ml, make to inspect soon that paper dries, sterile packed.