CN103190405A - Use of natural compound as agricultural bactericide or pesticide - Google Patents
Use of natural compound as agricultural bactericide or pesticide Download PDFInfo
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- CN103190405A CN103190405A CN2013101298460A CN201310129846A CN103190405A CN 103190405 A CN103190405 A CN 103190405A CN 2013101298460 A CN2013101298460 A CN 2013101298460A CN 201310129846 A CN201310129846 A CN 201310129846A CN 103190405 A CN103190405 A CN 103190405A
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- auraptenol
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Abstract
The invention discloses use of a natural compound Auraptenol as an agricultural bactericide or pesticide. The Auraptenol can be taken as the pesticide for preventing and treating Lepidoptera and homopetera insects or the bactericide for preventing and treating agricultural fungus diseases, and especially has remarkable control efficiency for sclerotinia rot of colza, fusarium wilt of cucumber, rice sheath blight disease, scab of wheat, anthracnose of hot pepper, rice bakanae disease, wheat sharp eyespot, tomato late blight, gray mold and the like. The Auraptenol as the agricultural bactericide or pesticide is good in effect, broad in spectrum, low in toxicity, free from residue, non-pollution and nuisanceless, and is especially in favor of environment protection.
Description
Technical field
The invention belongs to agricultural technology field, be specifically related to a kind of native compound Auraptenol prepares agricultural fungicides or bactericide as active component purposes.
Background technology
Pest are taken place in the many regional large tracts of land of China, and frequency is in rising trend, and harm increases the weight of.Still be the major control measure at present with the chemical pesticide control.Because the long-term chemical pesticide control that uses, cause insect that number of chemical insecticides such as organochlorine, organic phosphor, carbamate, pyrethroid have been produced pesticide resistance in various degree, control efficiency significantly descends, make the peasant have to strengthen dosage to improve preventive effect, still reduced the safety of agricultural product again.
In addition, in the agricultural production, the pathogen of damage to crops also has fungi, bacterium, virus etc., having the greatest impact of fungi wherein, and crop production reduction is obvious, because plant disease especially be can not be ignored by the economic loss that fungus-caused disease causes.
Auraptenol, CAS number: 1221-43-8, chemical name: (+)-8-(2-Hydroxy-3-methyl-3-butenyl)-7-methoxy-2H-1-benzopyran-2-one, molecular structure:
Auraptenol is colourless acicular crystal, is dissolved in organic solvents such as methyl alcohol, ethanol, acetone and chloroform, to its activity research seldom, and the pertinent literature or the report that do not have it to use in agricultural as bactericide or insecticide so far.
Summary of the invention
The objective of the invention is to explore Auraptenol as the application of active component at preparation agricultural fungicides or bactericide.
The present invention is by studies have shown that in a large number, and Auraptenol has excellent insecticidal activity as the agricultural fungicides that active component prepares, and can be used for preventing and treating various pest on crop, especially for control Lepidoptera and Homoptera insect.Lepidopterous insects can be Noctuidae, diamond-back moth section, Sulfur butterfly insect; Described noctuid is preferably prodenia litura, beet armyworm, mythimna separata; Diamond-back moth section insect is preferably diamond-back moth; The Sulfur butterfly insect is preferably cabbage caterpillar.Described Homoptera insect is the Aphidiadae insect, is preferably black peach aphid.
The agricultural bacteriocide that Auraptenol prepares as active component also has excellent bactericidal activity, can be applicable to the disease on the various crops of control, be particularly suitable for preventing and treating agricultural fungal diseases, as: sclerotinia rot of colza, cucumber fusarium axysporum, rice sheath blight disease, wheat scab, pepper anthracnose, bakanae disease of rice, wheat sharp eyespot, tomato late blight, gray mold etc.
Auraptenol is that separation and purification obtains or synthetic can getting from natural plants.Auraptenol is mixed with any one formulation that allows on the agricultural chemicals as active component and insecticides adjuvant, excipients.All right and other agriculture chemical compounding of Auraptenol is mixed and made into any one formulation that allows on the agricultural chemicals with insecticides adjuvant, excipients.Described formulation can be missible oil, suspending agent, aqueous emulsion, wetting powder, microemulsion or water dispersion granule etc.Used auxiliary agent can adopt calcium dodecyl benzene sulfonate, pyrrolidones, polyoxyethylene polyoxy third rare block compound, polyoxyethylene nonylphenol ether etc., and excipient can be cyclohexanone, solvent naphtha, solvent naphtha, water, propane diols, deionized water etc.Composite other used agricultural chemicals are organic phosphates, as: thimet (3911), demeton (1059, E1059), parathion (1605, E605), dichlorvos (DDV), Rogor, chlorophos etc.; Pyrethroid, as: cypermethrin, decamethrin, kill bacterium fat etc.; The microbial source insecticide is as bacillus thuringiensis,Bt (Bt), Avermectin, white muscardine fungi etc.
Beneficial effect of the present invention: Auraptenol is as insecticide or bactericide compared with the prior art, and insecticidal spectrum, antimicrobial spectrum are wide, and desinsection, antibacterial time are short, and the lasting period is long, is insecticide, the bactericide of agriculture coideal.Compare with general chemical pesticide; insect had tag, stomach poison function, effect is remarkable, and Auraptenol derives from the extract of natural plants; have low toxicity, noresidue, pollution-free, non-harmful characteristics for economic crops such as various grains, melon and fruit, vegetables, more favorable environment protection.
Embodiment
Below by embodiment effect of the present invention is described further:
The control of the agricultural fungal diseases of embodiment 1:Auraptenol
Strains tested Fusarium graminearum (Fusarium.graminearm) obtains from the separation of wheat scab fringe; Fusarium oxysporum bacterium (F.oxysporum) obtains from the separation of cucumber fusarium axysporum diseased plant; Rhizoctonia solani Kuhn (Rhizoctonia solani) obtains from the separation of rice sheath blight disease leaf; Cereal rhizoctonia (Rhizoctonia solani) obtains from the separation of wheat sharp eyespot leaf; Sclerotinite (Sclerotioia selerotiorum) obtains from the separation of sclerotinia rot of colza bacterial strain; Fusarium moniliforme (F.moniliform) separates from the bakanae disease of rice fringe; Capsicum anthrax-bacilus (Colletotrichum capsici) separates from the sick leaf of capsicum; Botrytis cinerea (Botrytis cinerea) separates from cucumber; Alternaria solani sorauer bacterium (Alternariasolani) separates from the sick leaf of early blight of tomato; Phytophthora capsici (phytophthora capsici) separates from the capsicum diseased plant.Reagent agent Auraptenol (separating evaluation from the samphire root of Dahurain angelica obtains) is dissolved in acetone with it and makes 1000 μ g/mL mother liquors, and 4 ℃ of preservations are standby.PDA medium: 200g peeling potato is cut into piece, boil 10min after, get juice, add 20g agar, be heated to fusing, add 20g glucose again, water is settled to 1000mL.
Adopt the mycelial growth rate determination method that the inhibition of hypha,hyphae growth is measured, pre-incubated fungi edge is beaten and is got bacterium dish (diameter 5mm) from the PDA flat board, the reagent agent mother liquor is evenly mixed with medium, make that the reagent agent ultimate density is respectively 0,1,2.5,5,10ppm, mixed liquor impouring diameter respectively is to make flat board in the sterilization culture dish of 9cm, every ware is inoculated a bacterium dish, and mycelia faces up, and every processing repeats 3 times.25 ℃ are cultured to the contrast bacterium colony and are covered with culture dish 2/3rds when above, and the right-angled intersection method is measured bacterium colony growth diameter.Calculate growth inhibition ratio by colony diameter mean value (mm).Data to gained are handled with DPS V3.01 professional version software analysis, draw calibration curve and EC
50Value.Positive reference substance is Osthole (available from Nat'l Pharmaceutical ﹠ Biological Products Control Institute), calculates the conk inhibiting rate with same concentration with quadrat method.
Auraptenol can prevent and treat sclerotinia rot of colza, cucumber fusarium axysporum, rice sheath blight disease, wheat scab, pepper anthracnose, bakanae disease of rice, wheat sharp eyespot, tomato late blight, gray mold etc. effectively as can be seen from the result, concentration EC during these several fungal diseases are suppressed
50Be 2.84～18.46ppm, concrete experimental result sees Table 1; And the positive reference substance Osthole is to concentration EC in sclerotinia rot of colza, cucumber fusarium axysporum, rice sheath blight disease, wheat scab, these 5 kinds of fungal diseases inhibition of wheat sharp eyespot
50Be respectively 43.33,41.82,26.84,56.94,39.96ppm, demonstrated fully the remarkable result of agricultural chemicals of the present invention by the contrast experiment.
Table 1Auraptenol is to agriculture fungi toxicity test result
Embodiment 2:Auraptenol as active component to agricultural insect management
Lay eggs to pupating in indoor artificial feed raising for field population 3～6 instar larvaes of gathering for trying the worm source, select indoor F1 and F2 and use (wherein, cabbage caterpillar is that population is gathered in the field) for the larva of big or small basically identical for toxicity test.Accurately take by weighing Auraptenol (separate identify obtain) from the samphire root of Dahurain angelica, it be dissolved in acetone, make concentration and be 2.000mg/ml for the examination mother liquor, it is standby as supplying reagent liquid to be diluted to 6 different concentration with 1 ‰ TritonX-100 aqueous emulsions then.With defoliator with fresh clean cabbage leaves (wherein, mythimna separata is fresh tea leaf in its with wheat leaf blade, tea geometrid) make the leaf dish that diameter is 1.5cm, take out after then it being soaked 10 seconds in for reagent liquid, the back is taken out to dry and is placed the culture dish that contains 1% water agar or the filter paper of preserving moisture, insert the examination worm of hungry 3h, place artificial climate indoor [(25 ± 1) ℃, L/D=14/10, RH75%], the soup of each concentration is handled 30 cephalonts.The blank group adds 1 ‰ Triton100 aqueous emulsions dipping blade with acetone, and positive reference substance is Osthole (available from Nat'l Pharmaceutical ﹠ Biological Products Control Institute), handles the examination worm with the same leaf dipping method of same concentration.48h checks lethality, calculates each dead borer population of handling, borer population alive, calculates larval mortality.
The result shows: in the 48h, reagent agent is effective to prodenia litura, beet armyworm, diamond-back moth, cabbage caterpillar, mythimna separata, black peach aphid, and concrete lethality sees Table 2, has tested the virulence activity to the anomala corpulenta adult in addition, and it is invalid to chafer that the result shows; And positive controls, the lethality that examination worm prodenia litura, beet armyworm, mythimna separata, the black peach aphid that concentration is handled for the 1.000mg/ml Osthole tags is all less than 40%.Show that by comparative trial Auraptenol has the advantage that the desinsection time is short, the desinsection scope is wide as agricultural insecticide.
The virulence test result of the insect of table 2Auraptenol
Embodiment 3:
Take by weighing conventional auxiliary materials such as Auraptenol and lignin, diatomite according to common process, make active ingredient concentration and be 2% wetting powder.
Embodiment 4:
Take by weighing Auraptenol and calcium dodecyl benzene sulfonate, phenethyl phenol polyethenoxy ether, the conventional auxiliary material of solvent wet goods according to common process, make active ingredient concentration and be 2% missible oil.
Embodiment 5:
Claim conventional auxiliary materials such as Auraptenol and wooden sodium sulfonate, kaolin according to common process, make active ingredient concentration and be 2% water dispersible granules.
Embodiment 6:
Take by weighing Auraptenol and solvent naphtha, pyrrolidones, conventional auxiliary material such as xanthans is according to common process, makes active ingredient concentration and be 1% aqueous emulsion.
Embodiment 7:
Take by weighing conventional auxiliary materials such as Auraptenol and calcium dodecyl benzene sulfonate, pyrrolidones according to common process, make active ingredient concentration and be 1% microemulsion.
Embodiment 8:
Take by weighing Auraptenol and thimet according to 1: 5 ratio of weight ratio, with solvent naphtha, pyrrolidones, conventional auxiliary material such as xanthans is according to common process, makes active ingredient concentration and be 12% aqueous emulsion.
Embodiment 9:
Take by weighing Auraptenol and decamethrin according to 6: 1 ratio of weight ratio, with solvent naphtha, pyrrolidones, conventional auxiliary material such as xanthans is according to common process, makes active ingredient concentration and be 21% aqueous emulsion.
Embodiment 10:
Take by weighing Auraptenol and Avermectin according to 1: 3 ratio of weight ratio, with solvent naphtha, pyrrolidones, conventional auxiliary material such as xanthans is according to common process, makes active ingredient concentration and be 4% aqueous emulsion.
Claims (10)
1.Auraptenol as the purposes of active component in preparation agricultural fungicides or bactericide.
2. purposes according to claim 1 is characterized in that Auraptenol is mixed with any one formulation that allows on the agricultural chemicals as active component and insecticides adjuvant, excipients; Perhaps Auraptenol is mixed with any one formulation that allows on the agricultural chemicals as active component and other agricultural chemicals and insecticides adjuvant, excipients.
3. purposes according to claim 2 is characterized in that described formulation is missible oil, suspending agent, aqueous emulsion, wetting powder, microemulsion or water dispersion granule; Described other agricultural chemicals are organophosphorus insecticide, pyrethroid pesticide or microbial source insecticide.
4. purposes according to claim 1 is characterized in that described insecticide is used for control Lepidoptera and Homoptera insect.
5. purposes according to claim 4 is characterized in that described lepidopterous insects is noctuid, diamond-back moth section insect, Sulfur butterfly insect.
6. purposes according to claim 5 is characterized in that described noctuid is prodenia litura, beet armyworm, mythimna separata; Described diamond-back moth section insect is diamond-back moth; Described Sulfur butterfly insect is cabbage caterpillar.
7. purposes according to claim 4 is characterized in that described Homoptera insect is the Aphidiadae insect.
8. purposes according to claim 7 is characterized in that described Aphidiadae insect is black peach aphid.
9. purposes according to claim 1 is characterized in that Auraptenol is used for preventing and treating agricultural fungal diseases as bactericide.
10. application according to claim 9 is characterized in that described agricultural fungal diseases is sclerotinia rot of colza, cucumber fusarium axysporum, rice sheath blight disease, wheat scab, pepper anthracnose, bakanae disease of rice, wheat sharp eyespot, tomato late blight, gray mold.
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| CN201310129846.0A CN103190405B (en) | 2013-04-16 | 2013-04-16 | Use of natural compound as agricultural bactericide or pesticide |
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| CN201410009288.9A Division CN103858874B (en) | 2013-04-16 | 2013-04-16 | Application of natural compound as agricultural bactericide |
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| CN103190405B CN103190405B (en) | 2014-05-21 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108157393A (en) * | 2017-12-13 | 2018-06-15 | 中央民族大学 | A kind of Angelica Dahurica extract and its application as Genes For Plant Tolerance fungus |
Citations (2)
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| CN101456853A (en) * | 2009-01-05 | 2009-06-17 | 江苏省农业科学院 | Bionic novel series compounds 7-alkoxyl-8-(3,3'-disubstituted propyl) benzo pyran-2-ones synthesis and its application as pesticides |
| CN102093322A (en) * | 2010-12-31 | 2011-06-15 | 厦门大学 | Application of osthole in preparation of marine antifouling paint |
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101456853A (en) * | 2009-01-05 | 2009-06-17 | 江苏省农业科学院 | Bionic novel series compounds 7-alkoxyl-8-(3,3'-disubstituted propyl) benzo pyran-2-ones synthesis and its application as pesticides |
| CN102093322A (en) * | 2010-12-31 | 2011-06-15 | 厦门大学 | Application of osthole in preparation of marine antifouling paint |
Non-Patent Citations (5)
| Title |
|---|
| BIKASH RANJAN BARIK: "《coumarins of murraya exotiva -absolute configuration of auraptenol》", 《PHYTOCHEMISTRY》 * |
| W.L.STANLEY: "《auraptenol,a coumarin compound in bitter orange oil》", 《TETRAHEDRON》 * |
| 吴寿金、赵泰等: "《现代中草药成分化学》", 30 December 2002 * |
| 陈艳: "《蛇床子的化学成分及药理作用的研究进展》", 《沈阳药科大学学报》 * |
| 韦献果: "《柑橘及其近缘属植物中天然香豆素化合物及药效作用综述》", 《食品科学》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108157393A (en) * | 2017-12-13 | 2018-06-15 | 中央民族大学 | A kind of Angelica Dahurica extract and its application as Genes For Plant Tolerance fungus |
| CN108157393B (en) * | 2017-12-13 | 2021-08-20 | 中央民族大学 | Angelica dahurica extract and application thereof as plant antibacterial |
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