CN103182393A - Method for regulating and controlling festuca arundinacea lawn soil nematode community structure by adopting complexant - Google Patents
Method for regulating and controlling festuca arundinacea lawn soil nematode community structure by adopting complexant Download PDFInfo
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Abstract
The invention discloses a method for regulating and controlling a festuca arundinacea lawn soil nematode community structure by adopting a complexant. The method comprises the following steps: adopting a plastic flowerpot with a height of 25 cm and a diameter of 12 cm, shearing a gauze into a circle with appropriate size to fix the bottom part of the flowerpot after cleaning and drying, sowing 4.5 g festuca arundinacea seeds in each container which weighs 2 kg, supplying water quantitatively everyday during the experiment to satisfy the need of the plant growth, adding EDTA ( Ethylene Diamine Tetraacetic Acid ),NTA ( Nitrilo Triacetic Acid ) or DTPA ( Diethylene Triamine Pentaacetic Acid ) with a concentration of 5 to 10 mmol /kg<-1>respectively, and culturing under the situations that the temperature is 25-34 DEG C, the relative humidity is 31-48%, the light condition is natural incident light, and the whole test lasts 120 days. The experiment result shows that the adoption of complexant increases the quantity of the soil nematodes on the whole, and particularly increases the quantity of the plant parasitic type nematodes; meanwhile the quantity of the bacteria eating type and polyphagia feeding type nematodes is reduced, thereby influencing the health of the soil ecosystem.
Description
Technical field
The invention belongs to environmental protection technical field, relate to particularly a kind of method that adopts complexing agent regulation and control tall fescue turf soil nematodes structure of community of urban afforestation.
Background technology
Phytoremediation is a kind of contaminated soil recovery technique of rising in recent years, owing to its environmental friendliness, economy, original position reparation, be applicable to advantage such as large tracts of land, has been subjected to paying close attention to widely and becoming the focus of environmental science research.Domestic and international in adding the influence research of complexing agent to plant at present, the application that the interpolation of relevant complexing agent is repaired the heavy metal pollution edaphophyte is also many.It is a very important restriction factor that the bioavailability of heavy metal hangs down in the process of plant absorbing heavy metal ion, especially in neutrality or basic soil.The collaborative big crops of some biomass down of organic complexing agent, for example heronsbill, corn, soybean and winter wheat more successfully are applied in the research of complexing phytoremediation.Research thinks that EDTA can improve the plant availability of rhizosphere soil Pb, promotes Pb transportation to ground portion from root system, significantly improves plant to the accumulation ability of Pb.
Complexing agent is the key point that complexing agent is applied to phytoremediation to the activation of heavy metal in soil.The activation of complexing agent mainly is to be combined by the heavy metal ion of complexing agent in the soil liquid, reduce the concentration of metal ions in the liquid phase of soil, for keeping the balance of metal ion between liquid phase and solid phase, heavy metal is from the soil particle surface desorption, be converted into soluble state by insoluble attitude, for the absorption of plant creates favorable conditions.Intercalating agent not only can improve enriching plant to the accumulation of heavy metal, and general crop is also had same effect.Complexing agent is the heavy metal in the activating soil effectively, and then promotes plant to the enrichment of heavy metal, therefore is widely used in fortification of plants to the extraction reparation of heavy metal in soil.After complexing agent was added in the soil, the effect of complexing agent can change the occurrence patterns of heavy metal in soil, improved the biological effectiveness of heavy metal, so can fortification of plants to the absorption of target heavy metal.As everyone knows, EDTA be a kind of very effective increase heavy metal at the complexing agent of soil and plant bulk concentration, so it is often used in the soil technology of phytoremediation heavy metal pollution.Much studies show that: the volume of plant roots, density and distribute can the appreciable impact heavy metal motion in soil and plant to the bioaccumulation efficiency of heavy metal, to the high resistance of EDTA and heavy metallic poison, it has very big potentiality and value in phytoremediation.
Soil and lawn are organic wholes, and the two supplements and complements each other, interacts.Soil system provides essential nutriment for the lawn plant growth, and the thing that withers and falls that the plant growth produces can improve the fertility state of soil.Edaphon is as the driving force of the soil organism and nutrient conversion.Soil nematodes occupies a plurality of trophic levels in the soil food web, account for the center in soil saprophage food web, and soil ecosystem is stable for keeping, the promotion material circulates and energy flows significant.But at present, relevant interpolation complexing agent is less relatively to the influence research of soil ecology feature, especially adopts the research of the method for complexing agent regulation and control tall fescue turf soil nematodes structure of community, does not see bibliographical information as yet.
Summary of the invention
The object of the present invention is to provide a kind of method that adopts complexing agent regulation and control tall fescue turf soil nematodes group.The present invention is that the lawn organizational system system of matrix is used common complexing agent at single soil on the basis of optimizing experiment, uses three kinds of common complexing agents, and realization will have important function to the regulating and controlling effect of tall fescue turf soil nematodes group.
For achieving the above object, the invention provides following technical scheme:
The application of complexing agent aspect preparation conduct regulation and control tall fescue turf soil nematodes group; Described complexing agent is disodium ethylene diamine tetraacetate, aminotriacetic acid misfortune diethyl triamine tetraacethyl.
Application of the present invention refers to the application of complexing agent aspect the quantity reduction food bacterium class that increases soil nematodes and omnivorous predation class nematode population.Wherein refer to complexing agent EDTA and NTA and handle the increase that the back promotes line insect number.
Employing complexing agent of the present invention is regulated and control the method for tall fescue turf soil nematodes group and is undertaken by following step:
(1) it is standby the pedotheque of gathering to be crossed 2 mm sieve mixing; Lawn plant is selected Festuca Arundinacea for use, and complexing agent selects for use disodium ethylene diamine tetraacetate, aminotriacetic acid, diethyl triamine tetraacethyl standby;
(2) plastic flowerpot of high 25 cm, diameter 12cm is adopted in test, will be cut into fixedly flowerpot bottom of sizeable circle behind the gauze cleaning, drying, and each container 2kg that weighs sows tall fescue seed 4.5g;
(3) experimental session quantitative water supply every day, required to satisfy plant growth, when growth of Festuca elata by 28-35 days, add concentration 5-10mmolkg respectively
-1EDTA, NTA or DTPA;
(4) temperature is 25-34 ℃ between culture period, and relative humidity is 31%-48%, and illumination condition is the nature incident light, and whole test is lasted 120 days, the Festuca Arundinacea aerial part is cradled in 60 days in experiment, stays the 1cm stubble, measures one batch of careless plant height and biomass; When finishing, experiment carries out the measurement of eldish plant height and biomass; Separate before the experiment beginning, identify for line insect types and quantity in the examination soil as nematode background data;
After (5) 4 months, separate evaluation and respectively handle kind and the quantity of nematode, be applied to the reparation of turf establishment system heavy metal to the influence of soil nematodes in the hope of understanding complexing agent.
The more detailed preparation method of the present invention is as follows:
1 materials and methods
1.1 experiment material and sampling method
Take from the institute of Tianjin Normal University for the examination pedotheque.Depth of soil is 0-20 cm, and it is standby that the pedotheque of collection is crossed 2 mm sieve mixing.Lawn plant select for use Festuca Arundinacea (
Festuca arundinacea L.), complexing agent is selected disodium ethylene diamine tetraacetate (ethylenediaminetetraacetic acid-Na for use
2), aminotriacetic acid (be called for short NTA), diethyl triamine tetraacethyl (be called for short DTPA.
The plastic flowerpot of high 25 cm, diameter 12cm is adopted in test, will be cut into fixedly flowerpot bottom of sizeable circle behind the gauze cleaning, drying.Each container 2kg experiment purposes of weighing, sowing tall fescue seed 4.5 g.Experimental session quantitative water supply every day, required to satisfy the plant growth.Plant growth regulation 28d adds EDTA, and EDTA concentration adopts 0,5,10,15 mmolkg
-14 processing horizontals are with 0 mmolkg
-1EDTA is contrast, and test is 3 repetitions.Plant is cultivated and carries out in the laboratory, and temperature is 25-34 ℃ between culture period, and relative humidity is 31%-48%, and illumination condition is the nature incident light, and whole test is lasted 120 d.Cradle at the Festuca Arundinacea aerial part of experiment 60 d, stay the 1cm stubble, measure one batch of careless plant height and biomass.When finishing, experiment carries out the measurement of eldish plant height and biomass.Separate before the experiment beginning, identify for line insect types and quantity in the examination soil as nematode background data.After 4 months, separate evaluation and respectively handle kind and the quantity of nematode, be applied to the reparation of turf establishment system heavy metal to the influence of soil nematodes in the hope of understanding complexing agent.
1.2 the separation of soil nematodes, authentication method
Each soil sample takes by weighing 100g, adopts to eluriate-sieve-sucrose centrifugal process separation nematode.According to soil moisture, the soil nematodes population quantity is converted to the number that per 100 g dry ground contain nematode.
According to the head morphological feature of nematode with get the food habitat soil nematodes is divided into following 4 nutrition monoids: food bacterium class, food Mycophyta, phytotrophy class and predation/Omnivora.The nematode classification identifies section's genus level.Adopt the diversity indices of soil nematodes ecologist widespread usage to measure the soil nematodes community diversity.
Three basic quantizating index are respectively: N is the individual number of identifying; S is for identifying the number of taxon, and a certain given taxon can be regarded as i taxon; P
iIt is individual shared ratio in i the taxon.Can calculate following cardinal index according to above-mentioned three indexs:
Diversity indices (Diversity) H '=-Σ P
iLn P
i
Evenness index (Evenness) J=H '/H '
Max, H ' wherein
Max=lnS
Diversity index (Richness) SR=(S-1)/lnN
Dominance index (Dominance) λ=Σ P
i 2
1.3 data statistics and analysis
The data MICROSOFT EXCEL 2007 and SPSS17.0 software carry out significance test and correlation analysis.
2 development results and analysis
2.1 complexing agent is to the influence of one batch of careless biomass of Festuca Arundinacea
Three processing of NTA all have in various degree facilitation to the ground fresh weight in this experiment, wherein with 10 mmolkg
-1The processing effect is the most remarkable, when 60 d its on the ground fresh weight be 162.0% of contrast, dry weight is 185.3% of contrast on the ground, fresh and dried ratio is 90.9% of contrast.The meaning of fresh and dried ratio is with reference to chapter 2.Experiment phase I three kinds of complexing agents are to the existing significantly influence of Festuca Arundinacea form growth, and the influence of variable concentrations is identical with the chapter 2 general trend.
Table 1 complexing agent is to the influence (g/column) of one batch of careless biomass of Festuca Arundinacea
2.2 complexing agent is to the influence of Festuca Arundinacea eldish biomass
Cradle at the Festuca Arundinacea aerial part of experiment the 120th d, measure the regrowth hair biomass.Observe the growing state of eldish during this period, the one batch of grass of comparing, the eldish growing way is slow, and plant is very thin, and color is more shallow, and the plant water requirement is starkly lower than one batch of grass growth required water.According to table 2 as can be known, three processed group of EDTA are fresh weight on the ground, and the control group of comparing all has reduction in various degree, is respectively 62.3%, 57.9% and 58.8% of contrast.On the ground dry weight all be starkly lower than contrast (
P＜0.05).5 mmolkg
-1With 10 mmolkg
-1NTA handles contrast increasing by a small margin, improves 28.5% and 18.4% respectively.Dry weight is except 5 mmolkg on the ground
-1Handle, other handle also be starkly lower than contrast (
P＜0.05).DTPA eldish growing way obviously is weaker than other two kinds of complexing agents, finishes in experiment, and three processed group ground fresh weights only are 75.0%, 58.8% and 32.9% of contrast.
Table 2 complexing agent is to the influence (g/column) of Festuca Arundinacea eldish biomass
2.3 background soil nematodes quantity, section belong to classification and nutrition monoid
The division number of individuals of each monoid predominance degree accounts for total quantity of the catch 10% above person and is dominant groups, and number of individuals accounts for total quantity of the catch 1%～10% and is common monoid, and number of individuals accounts for total quantity of the catch and is rare monoid below 1%.Before testing beginning quantity and section's genus classification for the examination soil nematodes have been carried out recording (table 3), observe 1570.11 of soil nematodes line insect numbers altogether, 9 sections 21 belong to, and wherein the spiral of phytotrophy monoid genus and the genus that spirals are dominant generas, and relative aboundance accounts for 79.8% and 11.4% of soil nematodes respectively.Nematode population is 2.3～1252.87 in the every 100g dry ground of background soil.Wherein the genus of minimum number is for the deer horn lip belongs to, stem belongs to, downgrade genus, and maximum is that spiral belongs to.
In background soil nematodes group nutrition monoid, phytotrophy monoid absolute abundance is the highest, and is omnivorous-predation monoid absolute abundance minimum (table 3).Illustrate that the Parasita nematode to the plant hazard maximum is preponderated in the background soil nematodes.
Nematode density in the table 3 background soil (individual/100 g soil), group are formed, relative abundance (RA, %) and dominance
Annotate: +++, RA〉10 dominant genera Dominant genus; ++, 1≤RA≤10 common genus Ordinary genus; +, RA＜1, rare genus Rare genus
3 discuss
3.1 complexing agent is used the influence to one batch of Festuca Arundinacea, regrowth hair biomass
Each complexing agent variable concentrations is identical with experimental result trend cardinal principle in the chapter 2 to the influence of one batch of careless biomass of Festuca Arundinacea by analysis, does not repeat them here.After one batch of grass of plant (60 d) cradles, keep 1 cm stubble, test 120 d and measure the regrowth hair biomass.The heavy metal kind difference of selected EDTA, NTA, DTPA activation in the experiment is also different in the half-life of soil effect.Wherein with time half-life of NTA for the shortest, the experiment second stage is used each processed group of NTA, and increase is by a small margin arranged compared with the control, may fade away relevant with the drug effect of NTA.Along with the growth of experimental period, the reinforcement of soil aging effect, the biological effectiveness of three kinds of complexing agents descends, thus make and contrast between otherness reduce gradually.
3.2 complexing agent belongs to the influence of classification and nutrition monoid to lawn soil nematode population, section
Table 4 showed experiment after 4 months, and EDTA uses the situation of change to nematode population in the soil and section's genus.EDTA 5 mmolkg
-1In the processing, observe soil nematodes 9 sections 14 and belong to, the kind level obviously is less than background soil, and each nutrition monoid of nematode group is formed all and reduced to some extent, and dominant genera still is that the spiral of phytotrophy monoid belongs to, and relative aboundance reaches 88.8%, be significantly higher than background soil (
p＜0.05); 10 mmolkg
-1In the processing, observe soil nematodes 5 sections 8 and belong to, dominant genera is that the spiral of phytotrophy monoid belongs to and food bacterium class plate lip belongs to, and relative aboundance reaches 75.51% and 10.20% respectively, but soil nematodes adds up to 233.33, obviously is less than background soil.15 mmolkg
-1In the processing, 1630 of soil nematodes sums belong to 8 sections 14 and belong to.The spiral that dominant genera is the phytotrophy class belongs to, and section belongs to kind and is starkly lower than background soil, three handle soil nematodes omnivorous-the predation monoid disappears.
In three processing of NTA (table 5), observe respectively that nematode 10 sections 20 belong to, 11 sections 17 belong to and 7 sections 14 belong to, spiral belongs to and is sociales, and relative aboundance reaches 89.97%, 90.04% and 90.46%(respectively
p＜0.05).All the other are rare monoid.The background soil of comparing after three concentration are handled all reduces the short body section of phytotrophy class and hymeniderm section, 5 mmolkg
-1With 10 mmolkg
-1Handle line insect number and be respectively 2924.03 and 1944.72, apparently higher than background soil, and all increase phytotrophy class ring section and food fungi comparison Aphelenchidae; 15 mmolkg
-1It is less that processing section genus level is compared other two processing.NTA handles four kinds of nematode types of soil nematodes and all exists, and has promoted the increase of food Mycophyta and omnivorous-predation class section genus.
DTPA(table 6 in this research) three horizontal processing nematode sections belong to than the contrast minimizing obviously, and the soil nematodes sum also obviously reduces.5 mmolkg wherein
-1Only save as 3 sections 6 in the processing and belong to, food Mycophyta and food bacterium class all disappear.Phytotrophy class spiral genus and the genus that spirals were sociales during each was handled, and common kind is that food bacterium class plate lip belongs to and little bar genus, and eating bacterium monoid nematode mainly is food with the bacterium, to not directly injury of plant.If all the other each owner's phytotrophy classes, and phytotrophy monoid nematode is food with the host plant, to the injury maximum of plant.Above presentation of results, there is notable difference in different complexing agents to the influence of nematode group, but all promoted colony's quantity of phytotrophy monoid in various degree, the nutrition monoid of non-plant parasitism has then been had in various degree effect, especially promoted growing of food bacterium monoid.Therefore, three kinds of complexing agents are used the growth of the back tired colony of soil nematodes phytotrophy quantity, and the reduction of non-plant parasitic nematode nutrition monoid illustrates that the complexing agent application has influenced soil quality.From the soil animal angle, this result has reflected that also lawn system complexing agent is applied to may change soil microflora in the heavy metal reparation, suppressed beneficial microorganism growth in the soil, thereby promoted colony's quantity of plant nematode, increased the harm of soil nematodes to plant.
During table 4 EDTA handles nematode density (individual/100 g soil), group form, relative abundance (RA, %) and dominance
During table 5 NTA handles nematode density (individual/100 g soil), group form, relative abundance (RA, %) and dominance
During table 6 .DTPA handles nematode density (individual/100 g soil), group form, relative abundance (RA, %) and dominance
3.3 complexing agent is to the influence of lawn soil nematode community diversity
Ecosystem function is considered to the important factor that population continues and a certain specified level bio-diversity is kept; Bio-diversity is an important factor of ecosystem process stability, and wherein different species are being brought into play different effects with population, thereby the forfeiture of a certain special species or population may cause the forfeiture of ecosystem function.The comparatively general application diversity indices of present most soil ecology scholar is measured the diversity of soil nematodes group.Originally discover when experiment is final, diversity indices and the diversity index of soil nematodes all were lower than background soil (table 7) during three kinds of complexing agents were handled, explanation was through 4 months growth and breeding, and complexing agent is used and suppressed the variation of soil nematodes and the uniformity of kind; Three kinds of complexing agents are used the back dominance and all are higher than background soil, and EDTA uses has small size raising than background soil, and NTA and DTPA evenness index descend, but difference is not remarkable.
Table 7 complexing agent is to the influence of lawn soil nematode bio-diversity
4 development conclusions
In this experiment, through 4 months growth and breedings and the alternation of generations, nematode group is showing significant difference aspect quantity, composition structure and the bio-diversity in the soil that complexing agent is used.Line insect number has different the variation than background soil in the soil after complexing agent is used, and wherein EDTA and NTA processing is facilitation to the increase of line insect number, but belongs to the level affects difference in section.Though not generation and the disappearance of equal genus are arranged after three kinds of processing, do not change the sociales kind of soil, namely phytotrophy class spiral belongs to real dominant genera still.Increased food Mycophyta and food bacterium class section genus after NTA handles, the plant growth has been had certain facilitation.Food fungoid nematode and omnivorous-predatism nematode are the nutrition monoids of negligible amounts in this research.DTPA belongs to quantity for the section that reduces the phytotrophy monoid and has certain effect, but still can not change the status that spiral belongs to dominant genera.In a word, use complexing agent to increase quantity, the especially quantity of phytotrophy class nematode of soil nematodes substantially, reduced the quantity of food bacterium class and omnivorous predation class simultaneously, thereby the health of soil ecosystem is exerted an influence.
The specific embodiment:
The present invention will be further described below in conjunction with specific embodiment, and following each embodiment only is used for explanation the present invention and is not limitation of the present invention.
Embodiment 1
(1) it is standby the pedotheque of gathering to be crossed 2 mm sieve mixing; Lawn plant is selected Festuca Arundinacea for use, and complexing agent selects for use disodium ethylene diamine tetraacetate, aminotriacetic acid or diethyl triamine tetraacethyl standby;
(2) plastic flowerpot of high 25cm, diameter 12cm is adopted in test, will be cut into fixedly flowerpot bottom of sizeable circle behind the gauze cleaning, drying, and each container 2kg that weighs sows tall fescue seed 4.5 g;
(3) experimental session quantitative water supply every day, required to satisfy plant growth, when growth of Festuca elata by 28-35 days, add concentration 5-10mmolkg respectively
-1EDTA, NTA or DTPA;
(4) temperature is 25 ℃ between culture period, and relative humidity is 31%, and illumination condition is the nature incident light, and whole test is lasted 120 days, the Festuca Arundinacea aerial part is cradled in 60 days in experiment, stays the 1cm stubble, measures one batch of careless plant height and biomass; When finishing, experiment carries out the measurement of eldish plant height and biomass; Separate before the experiment beginning, identify for line insect types and quantity in the examination soil as nematode background data;
After (5) 4 months, separate evaluation and respectively handle kind and the quantity of nematode, be applied to the reparation of turf establishment system heavy metal to the influence of soil nematodes in the hope of understanding complexing agent.
Embodiment 2
(1) it is standby the pedotheque of gathering to be crossed 2 mm sieve mixing; Lawn plant is selected Festuca Arundinacea for use, and complexing agent selects for use disodium ethylene diamine tetraacetate, aminotriacetic acid or diethyl triamine tetraacethyl standby;
(2) plastic flowerpot of high 25cm, diameter 12cm is adopted in test, will be cut into fixedly flowerpot bottom of sizeable circle behind the gauze cleaning, drying, and each container 2kg that weighs sows tall fescue seed 4.5g;
(3) experimental session quantitative water supply every day, required to satisfy plant growth, when growth of Festuca elata by 28-35 days, add concentration 5-10mmolkg respectively
-1EDTA, NTA or DTPA;
(4) temperature is 34 ℃ between culture period, and relative humidity is 48%, and illumination condition is the nature incident light, and whole test is lasted 120 days, the Festuca Arundinacea aerial part is cradled in 60 days in experiment, stays the 1cm stubble, measures one batch of careless plant height and biomass; When finishing, experiment carries out the measurement of eldish plant height and biomass; Separate before the experiment beginning, identify for line insect types and quantity in the examination soil as nematode background data;
After (5) 4 months, separate evaluation and respectively handle kind and the quantity of nematode, be applied to the reparation of turf establishment system heavy metal to the influence of soil nematodes in the hope of understanding complexing agent.
Claims (4)
1. the application of complexing agent aspect preparation conduct regulation and control tall fescue turf soil nematodes group; Described complexing agent is disodium ethylene diamine tetraacetate, aminotriacetic acid misfortune diethyl triamine tetraacethyl.
2. the described application of claim 1 refers to the application of complexing agent aspect the quantity reduction food bacterium class that increases soil nematodes and omnivorous predation class nematode population.
3. the described application of claim 1 wherein refers to complexing agent EDTA and NTA and handles the increase that the back promotes line insect number.
4. the described application of claim 1, wherein adopt the method for complexing agent regulation and control tall fescue turf soil nematodes group to be undertaken by following step:
(1) it is standby the pedotheque of gathering to be crossed 2 mm sieve mixing; Lawn plant is selected Festuca Arundinacea for use, and complexing agent selects for use disodium ethylene diamine tetraacetate, aminotriacetic acid, diethyl triamine tetraacethyl standby;
(2) plastic flowerpot of high 25 cm, diameter 12cm is adopted in test, will be cut into fixedly flowerpot bottom of sizeable circle behind the gauze cleaning, drying, and each container 2kg that weighs sows tall fescue seed 4.5 g;
(3) experimental session quantitative water supply every day, required to satisfy plant growth, when growth of Festuca elata by 28-35 days, add concentration 5-10mmolkg respectively
-1EDTA, NTA or DTPA;
(4) temperature is 25-34 ℃ between culture period, and relative humidity is 31%-48%, and illumination condition is the nature incident light, and whole test is lasted 120 days, the Festuca Arundinacea aerial part is cradled in 60 days in experiment, stays the 1cm stubble, measures one batch of careless plant height and biomass; When finishing, experiment carries out the measurement of eldish plant height and biomass; Separate before the experiment beginning, identify for line insect types and quantity in the examination soil as nematode background data;
After (5) 4 months, separate evaluation and respectively handle kind and the quantity of nematode, be applied to the reparation of turf establishment system heavy metal to the influence of soil nematodes in the hope of understanding complexing agent.
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| CN103797923B (en) * | 2014-03-11 | 2016-01-13 | 天津师范大学 | A kind of modified Nano carbon improves the method for soil matrix turfgrass reviviscence |
| CN103814744B (en) * | 2014-03-11 | 2016-01-13 | 天津师范大学 | A kind of modified Nano carbon is to the multifarious regulate and control method of soil nematodes |
| CN106069247A (en) * | 2016-06-21 | 2016-11-09 | 天津师范大学 | The method using carbon nanomaterial regulation and control lawn composting substrate nematode trophic group |
| CN106105855A (en) * | 2016-06-21 | 2016-11-16 | 天津师范大学 | A kind of regulate and control lawn composting substrate small arthropod trophic group method |
| CN109799326A (en) * | 2019-03-03 | 2019-05-24 | 中国科学院亚热带农业生态研究所 | A method of estimating different Feeding Habits Soil nematode biomass |
| CN109799326B (en) * | 2019-03-03 | 2021-12-14 | 中国科学院亚热带农业生态研究所 | Method for estimating biomass of nematodes in different feeding soil |
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