CN109799326A - A method of estimating different Feeding Habits Soil nematode biomass - Google Patents
A method of estimating different Feeding Habits Soil nematode biomass Download PDFInfo
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- 241000244206 Nematoda Species 0.000 title claims abstract description 133
- 239000002028 Biomass Substances 0.000 title claims abstract description 66
- 238000000034 method Methods 0.000 title claims abstract description 20
- 239000002689 soil Substances 0.000 title claims abstract description 19
- 238000005259 measurement Methods 0.000 claims abstract description 29
- 238000004364 calculation method Methods 0.000 claims abstract description 11
- 241000894006 Bacteria Species 0.000 claims abstract description 5
- 230000000877 morphologic effect Effects 0.000 claims abstract description 4
- 238000011156 evaluation Methods 0.000 abstract description 3
- 238000005457 optimization Methods 0.000 abstract description 2
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- 230000008878 coupling Effects 0.000 description 4
- 238000010168 coupling process Methods 0.000 description 4
- 238000005859 coupling reaction Methods 0.000 description 4
- 238000004173 biogeochemical cycle Methods 0.000 description 3
- 238000010586 diagram Methods 0.000 description 3
- 239000011521 glass Substances 0.000 description 2
- 238000005070 sampling Methods 0.000 description 2
- 238000007619 statistical method Methods 0.000 description 2
- 241000233866 Fungi Species 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 239000012141 concentrate Substances 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
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- HJUFTIJOISQSKQ-UHFFFAOYSA-N fenoxycarb Chemical compound C1=CC(OCCNC(=O)OCC)=CC=C1OC1=CC=CC=C1 HJUFTIJOISQSKQ-UHFFFAOYSA-N 0.000 description 1
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Abstract
The invention discloses a kind of method for estimating different Feeding Habits Soil nematode biomass, step: pedotheque A, is acquired, nematode, heat-killed nematode, fixed nematode, film-making is extracted and carries out Morphological Identification；B, nematode is divided into four feeding habits monoids according to retrieval foundation under the microscope, instant bacterium, fungivorous, phytophagous and omnivorous predatism, it takes pictures to every nematode and measurement of correlation software is used to measure maximum body breadth, nematode body length is not measured in the step, is estimated according to feeding habits by maximum body breadth；C, the biomass size of nematode is calculated separately according to the biomass calculation formula of different feeding habits monoids.Method is, to optimization and improvement that traditional evaluation method carries out, only need to measure nematode maximum body breadth according to the long linear relationship with maximum body breadth of different feeding habits nematode bodies, it is simple and easy to do, it is easy to operate, there is high accuracy, improve the efficiency of nematode biomass estimation.
Description
Technical field
The present invention relates to soil nematodes field of scientific study, it is more particularly to a kind of different Feeding Habits Soil nematodes biologies of estimation
The method of amount is suitable for the measuring and calculating of soil nematodes biomass.
Background technique
Soil nematodes is the type that quantity is most in multicellular organism, they are commonly distributed in the various ecosystems in the whole world
In.Soil nematodes plays an important role during ecosystem biogeochemical cycle, they are to environmental change response
Sensitivity plays great function in terms of as ecological indicator, and their feeding habits occupy the crucial link of food web extensively,
Soil nematodes receives significant attention in research fields such as biogeochemical cycle, Ecological indicator, food web trophic level relationships
(Bongers and Ferris,1999；Nneher,2010).The size of soil nematodes feeding habits composition and its biomass is to determine
The most important index (Ferris, 2010) of its contribution amount during biogeochemical cycle, traditional nematode biomass (W)
Estimation must be calculated by measurement nematode body long (L) and maximum body breadth (D), calculation formula is W=(L × D2)/
(1.6×106).However, often showing diversified body after carrying out heat-killed fixation to sample since nematode body is elongated
Type, this significantly increases the workload of the long measurement of nematode body.The measurement long compared to nematode body, the measurement ten of maximum body breadth
Divide simple and quick.Therefore, it is necessary to be improved to nematode biomass estimation method, to improve nematode biomass analysis and measurement
Working efficiency.
Summary of the invention
The purpose of the invention is to provide a kind of methods for estimating different Feeding Habits Soil nematode biomass.Method is easy
Row, easy to operate, this method is to utilize maximum body breadth according to the long extremely significant linear relationship with maximum body breadth of nematode body
(measurement is easy) (measurement is time-consuming) long to body is estimated, and then nematode biomass estimation formula is made to be reduced to one by two variables
A simpler variable easily surveyed, reaches and improves nematode biomass estimation efficiency, mentions for the correlative study of soil nematodes biomass
More convenient and effective evaluation method is supplied.
In order to achieve the above purpose, the present invention uses following technical measures:
Actual measurement obtains the soil line polypide length and maximum body breadth data of Different ecosystems, by nematode according to four feeding habits classes
Group's (food bacterium, fungivorous, phytophagous and omnivorous predatism) classification is simultaneously long to the body of each feeding habits related to maximum body breadth respectively
Relationship is for statistical analysis, the results showed that the body of each feeding habits is long to have extremely significant correlativity between maximum body breadth, so
Propose each feeding habits nematode body length and the wide fit equation of body on this basis afterwards, and then will be in the biomass estimation formula of nematode
Body length replaced with body is wide, propose the biology for each feeding habits nematode using maximum body breadth as unique measurement index respectively
Amount estimation formula.
A method of it estimating different Feeding Habits Soil nematode biomass, the steps include:
A, pedotheque is acquired, nematode, heat-killed nematode, fixed nematode, film-making is extracted and carries out Morphological Identification；
B, four feeding habits monoids, instant bacterium (Ba), food are divided into according to by nematode according to certain retrieval under the microscope
Fungi (Fu), phytophagous (He) and omnivorous predatism (OmPr) take pictures to every nematode and use the measurement of measurement of correlation software maximum
Body is wide (D, unit: μm).It is different from and body length and maximum body breadth is required while measured in traditional nematode biomass measuring and calculating technology
Technical requirements do not need in the step to measure nematode body length.Since the long measurement of nematode body is measured than nematode maximum body breadth
Consume more times, thus greatly improve nematode figure measure consumed by the time, by nematode sample par with
Nematode figure is averaged complexity estimation, and the average analysis time of single sample can save 60 minutes or more, greatly improve
The time efficiency of measurement；
C, biomass size (W, the unit: μ of nematode are calculated separately according to the biomass calculation formula of different feeding habits monoids
g).Each feeding habits nematode biomass calculation formula is as follows:
Bacterial-feeding nematodes biomass WBa=(19.192 × DBa 3)/(1.6×106)
Fungal-feeding nematode biomass WFu=(23.403 × DFu 3)/(1.6×106)
Phytophagous nematodes biomass WHe=(24.260 × DHe 3)/(1.6×106)
Omnivorous predatism nematode biomass WOmPr=(22.352 × DOmPr 3)/(1.6×106)
Only include one measurement index of nematode maximum body breadth in above formula, and includes that nematode body is long and most in existing formula
Substantially wide two measurement indexes are compared, and are simplified formula and are improved the time efficiency of nematode biomass measuring.
Compared with prior art, the present invention having the following advantages that and effect:
According to soil line polypide is long and the linear relationship of maximum body breadth, the optimization that traditional evaluation method is carried out and is changed
Into, nematode maximum body breadth only need to be measured, it is simple and easy to do, it is easy to operate, there is high accuracy, improve nematode biomass and estimate
Nematode biomass estimation formula is reduced to an index by two measurement indexes by the efficiency of calculation, and the measurement index is simpler
Singly it is easy to measure.Since there are certain relationships between nematode feeding habits monoid and biomass, proposed respectively for different feeding habits monoids
Corresponding biomass estimation formula, to ensure the precision of nematode biomass measuring and calculating.
Therefore, which can significantly improve the efficiency of nematode biomass measuring and calculating when guaranteeing estimation precision.
Detailed description of the invention
Fig. 1 is the body length and maximum body breadth fit correlation figure of a kind of different feeding habits monoid nematodes.
A, b, c, d respectively indicate bacterial-feeding nematodes, Fungal-feeding nematode, phytophagous nematodes and omnivorous predatism nematode in figure
Body is long and maximum body breadth is there are extremely significant linear relationship, their body long (y) can be fitted with maximum body breadth (x),
Its fit equation is respectively y=19.192x, y=23.403x, y=24.260x, y=22.352x.
Fig. 2 is a kind of comparison diagram of the invention that from the prior art different feeding habits nematode biomass are calculated with result difference.
Four feeding habits nematode biomass are calculated respectively with the prior art using the present invention, the results showed that the present invention
There is no notable difference with the results of measuring of the prior art, illustrates the precision present invention can ensure that calculating.
Fig. 3 is the time loss of a kind of present invention and prior art nematode biomass in the case where analyzing land use pattern
Comparison diagram.
Comprising 4 Land-Uses, respectively artificial herbage land, farmland, scondary forest and natural Inertial coupling in figure, indicate
There are significant difference, the present invention and the prior arts for the time loss of the present invention and the prior art when carrying out the measuring and calculating of nematode biomass
It compares, can be respectively the nematode biomass analysis saving of artificial herbage land, farmland, scondary forest and each sample of natural Inertial coupling
45,102,83 and 60 minutes.
Specific embodiment
Present invention will now be described in detail with reference to the accompanying drawings.: realizing that the schematic diagram of the principle of the present invention is as shown in Figure 1.
Embodiment 1:
A method of it estimating different Feeding Habits Soil nematode biomass, the steps include:
1) using earth boring auger using accidental sampling acquire pedotheque 500g, using modified Baermann funnel method (Barker,
1985) nematode is extracted, nematode sample is placed in 3 minutes or so kill nematodes in 60 DEG C or so water-baths, standing is cooled to room
8% formalin is added after temperature and shakes up, a drop concentrate will be taken to be placed on glass slide and add after nematode sample centrifugal concentrating
Lid coverslip carries out film-making.
2) nematode is identified at Nikon DIC differential interference microscope ECLIPSE 80i, according to actual needs according to
Certain retrieval foundation is divided into different feeding habits monoids to section/category/Zhong Houzai by nematode analysis to feeding habits or by nematode analysis,
Then it takes pictures to every nematode and measurement of correlation software is used to measure and record maximum body breadth (D, unit: μm), camera system is Buddhist nun
The DS-U3/L3-Vi1/Fi1/Fi2 of health.The Survey Software is iWorks.
3) the corresponding biological meter of feeding habits monoid according to the maximum body breadth (D) of every of measurement nematode, where selecting it
Formula is calculated, its biomass size (W) is calculated.Each feeding habits nematode biomass calculation formula is as follows:
Bacterial-feeding nematodes biomass WBa=(19.192 × DBa 3)/(1.6×106)
Fungal-feeding nematode biomass WFu=(23.403 × DFu 3)/(1.6×106)
Phytophagous nematodes biomass WHe=(24.260 × DHe 3)/(1.6×106)
Omnivorous predatism nematode biomass WOmPr=(22.352 × DOmPr 3)/(1.6×106)
The DBa 3、DFu 3、DHe 3、DOmPr 3Respectively bacterial-feeding nematodes, Fungal-feeding nematode, phytophagous nematodes and omnivorous catch
The cube of the maximum body breadth of feeding habits nematode, the method for the present invention, which need to only measure nematode maximum body breadth, can carry out estimating for biomass
It calculates, substantially increases the efficiency of nematode biomass estimation.
Embodiment 2:
A kind of method that estimating different Feeding Habits Soil nematode biomass is (with nematode biometric measurement under land use pattern
For the time loss comparison of amount), it the steps include:
1) accidental sampling is used using 5cm internal diameter earth boring auger, it is (artificial herbage land, farmland, secondary in four Land-Uses
Raw woods and natural Inertial coupling) 1kg pedotheque is acquired respectively in total 16 sample prescriptions；Using modified Baermann funnel method (Barker,
1985) nematode is extracted；Nematode sample after extraction is placed in 3 minutes kill nematodes in 60 DEG C of water-baths, standing is cooled to room
8% formalin is added after temperature to shake up；Sample placement 48 hours or more, nematode sample is concentrated with centrifuge, a drop is taken to be concentrated
Liquid, which is placed on glass slide and covers coverslip, carries out film-making.
2) Morphological Identification is carried out to nematode at Nikon differential interference microscope ECLIPSE80i, by nematode analysis to category
(genus) and it is divided into four feeding habits monoids (food bacterium, fungivorous, phytophagous and omnivorous predatism)；Nikon DS- is utilized simultaneously
Preceding 150 nematodes encountered when U3/L3-Vi1/Fi1/Fi2 camera system is to identification are taken pictures, and (nematode population is less than 150
Sample, take pictures to all nematodes), and with image analysis Survey Software iWorks measure and record nematode maximum body breadth
(D, unit: μm) He Tichang (L, unit: μm)；Record simultaneously consumed when the long measurement of maximum body breadth and body time (unit:
s)。
3) sample art methods (Andrassy, 1956；Ferris, 2010), most according to every of measurement nematode
Substantially wide (D) He Tichang (L), calculates its biomass size (W), and biomass calculation formula is W=(L × D2)/(1.6×106)。
It samples the method for the present invention and the feeding habits monoid pair where it is selected according to the maximum body breadth (D) of every of measurement nematode
The biomass calculation formula answered calculates its biomass size (W).Each feeding habits nematode biomass calculation formula is as follows:
Bacterial-feeding nematodes biomass WBa=(19.192 × DBa 3)/(1.6×106)
Fungal-feeding nematode biomass WFu=(23.403 × DFu 3)/(1.6×106)
Phytophagous nematodes biomass WHe=(24.260 × DHe 3)/(1.6×106)
Omnivorous predatism nematode biomass WOmPr=(22.352 × DOmPr 3)/(1.6×106)
Wherein DBa、DFu、DHeAnd DOmPrRespectively bacterial-feeding nematodes, Fungal-feeding nematode, phytophagous nematodes and omnivorous predatism
The maximum body breadth of nematode.
The method of the present invention and the prior art do not have notable difference (Fig. 2) to the biomass results of measuring of four feeding habits nematodes,
Illustrate present invention can ensure that calculating accuracy.
Calculate simultaneously and the time consumed when the long measurement of maximum body breadth and body carried out to each sample, with maximum body breadth measurement when
Between as time loss of the invention, using the sum of time of maximum body breadth and the long measurement of body as the time loss of the prior art,
T-test statistical method, the time loss of the comparison present invention and the prior art are sampled for each Land-Use respectively.As a result
Show that the present invention can be respectively the line of artificial herbage land, farmland, scondary forest and each sample of natural Inertial coupling than the prior art
The analysis of worm biomass is saved 45,102,83 and 60 minutes, the measuring and calculating time of soil nematodes biomass under land use pattern
It is whole to save nearly 20 hours (being calculated within 8 hours by work daily, the experiment is total to save 2.5 working days).
Claims (1)
1. a kind of method for estimating different Feeding Habits Soil nematode biomass, the steps include:
A, pedotheque is acquired, nematode, heat-killed nematode, fixed nematode, film-making is extracted and carries out Morphological Identification；
B, four feeding habits monoids are divided into according to by nematode according to retrieval under the microscope, instant bacterium, fungivorous, phytophagous and
Omnivorous predatism takes pictures to every nematode and measurement of correlation software is used to measure maximum body breadth, not to nematode body progress in the step
Row measurement is estimated according to feeding habits by maximum body breadth；
C, the biomass size of nematode, each feeding habits nematode biology are calculated separately according to the biomass calculation formula of different feeding habits monoids
It is as follows to measure calculation formula:
Bacterial-feeding nematodes biomass WBa=(19.192 × DBa 3)/(1.6×106)；
Fungal-feeding nematode biomass WFu=(23.403 × DFu 3)/(1.6×106)；
Phytophagous nematodes biomass WHe=(24.260 × DHe 3)/(1.6×106)；
Omnivorous predatism nematode biomass WOmPr=(22.352 × DOmPr 3)/(1.6×106)。
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| CN113740512A (en) * | 2021-09-06 | 2021-12-03 | 宁波大学 | Green ecological buoy field domestication method for farmland soil animal activity biosensor |
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