CN103181988A - Freeze-dried black nightshade for treating cancers - Google Patents
Freeze-dried black nightshade for treating cancers Download PDFInfo
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- CN103181988A CN103181988A CN2013100657733A CN201310065773A CN103181988A CN 103181988 A CN103181988 A CN 103181988A CN 2013100657733 A CN2013100657733 A CN 2013100657733A CN 201310065773 A CN201310065773 A CN 201310065773A CN 103181988 A CN103181988 A CN 103181988A
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Abstract
The invention relates to a medicine for treating tumors, which is prepared by utilizing freeze-dried black nightshade as a raw material. The medicine is characterized in that the whole herb of freeze-dried black nightshade or freeze-dried black nightshade fruit is utilized as a raw material to prepare the medicine for treating tumors, and the freeze-dried black nightshade fruit is preferably used as the raw material for preparing the medicine for treating the tumors; the antitumor activity of the medicine prepared by the whole herb of freeze-dried black nightshade and black nightshade fruit is higher than that of the medicine prepared by the whole herb of sundried black nightshade and black nightshade fruit.
Description
Technical field
The present invention relates to medicine, particularly a kind of lyophilizing Herba Solani Nigri for the treatment of cancer belongs to medical technical field.
Background technology
Herba Solani Nigri Solanum nigrumL. is Solanaceae (Solanaceace) Solanum annual herb plant.Another name Radix vitis romanetii, crow eyes grass, Herba Sonchi Oleracei, bitter certain herbaceous plants with big flowers.Herb of Herba Solani Nigri is used as medicine, and bitter in the mouth, cold in nature, little sweet, slightly poisonous is returned lung, stomach, urinary bladder channel, and the effect of heat-clearing and toxic substances removing, promoting blood circulation to remove blood stasis, inducing diuresis to remove edema, eliminating phlegm and stopping cough is arranged.Be used for sore and toxic, osteopatia sprain, chronic tracheitis, acute nephritis, skin eczema, dysuria, chronic senile tracheitis, leukorrhagia, prostatitis, diseases such as dysentery.Herba Solani Nigri and compound preparation thereof are widely used in treating all kinds of tumors clinically, are classified as one of ten big antitumor Chinese herbal medicine by Chinese medicine, and its antitumor action has caused the attention of research worker, and become the research focus of Chinese scholars.
We find through overtesting, and lyophilizing Herba Solani Nigri active anticancer is higher than the Herba Solani Nigri of drying.
Summary of the invention
The objective of the invention is the medicine that Herba Solani Nigri is the highly active treatment tumor of feedstock production.The object of the present invention is achieved like this, is the medicine of feedstock production treatment tumor with the lyophilizing Herba Solani Nigri.
Be the medicine of feedstock production treatment tumor with the lyophilizing Herba Solani Nigri, be characterised in that with lyophilizing herb of Herba Solani Nigri or lyophilizing Fructus Solani Nigri to be the medicine of feedstock production treatment tumor.
Of the present invention is the medicine of feedstock production treatment tumor with the lyophilizing Herba Solani Nigri, preferably is the medicine of feedstock production treatment tumor with the lyophilizing Fructus Solani Nigri.
Lyophilizing of the present invention is characterised in that with following technology lyophilizing herb of Herba Solani Nigri:
Bright herb of Herba Solani Nigri is cleaned roguing, is cut into segment; Sabot, sabot weight are 5-8 ㎏/㎡, are cooled to-20 ℃～– gradually and carry out pre-freeze for 60 ℃; It is 40～60Pa that sublimation drying is evacuated to system's absolute pressure, beginning heat temperature raising to 30 ℃～50 ℃, sublimation drying 15～24 hours; Parsing-desiccation keeps temperature of charge at 35 ℃～55 ℃, makes water content reach 2～3%, gets the lyophilizing herb of Herba Solani Nigri.
Lyophilizing of the present invention is characterised in that with following technology lyophilizing Fructus Solani Nigri: get Fructus Solani Nigri and wash; Sabot, loading amount are about 5-8Kg/ ㎡; Solidification point control is between-28～-32 ℃; After reaching the control temperature, open vacuum pump immediately and be evacuated to drying chamber pressure 40-50Pa, start heater switch then, promote distillation; The temperature of distillation should be controlled below 40 ℃; The temperature of resolution phase should be controlled below 45 ℃, when temperature of charge and heating-up temperature near the time dry the end, get the lyophilizing Fructus Solani Nigri.
The purpose of lyophilizing Herba Solani Nigri is to solve technical problems such as to dry loss of effective components bigger, and active anticancer is low.Directly preparing medicine of the present invention with bright Herba Solani Nigri in the Herba Solani Nigri harvest season can reduce cost.Accordingly, lyophilizing Herba Solani Nigri of the present invention the Herba Solani Nigri harvest season lyophilizing Herba Solani Nigri of the present invention can replace with bright Herba Solani Nigri, or the lyophilizing Fructus Solani Nigri can replace with bright Fructus Solani Nigri, its consumption is pressed the dry product conversion.
Of the present invention is the medicine of feedstock production treatment tumor with the lyophilizing Herba Solani Nigri, is on the basis of existing technology, adopts the Chinese medicine preparation conventional method to be prepared into any pharmaceutically useful preparation.For example, lyophilizing herb of Herba Solani Nigri or lyophilizing Fructus Solani Nigri are pulverized, made powder and take after mixing it with water; Lyophilizing herb of Herba Solani Nigri or lyophilizing Fructus Solani Nigri are extracted by further, and extract adds adjuvant and makes tablet, capsule, granule, pill, but this does not limit protection scope of the present invention.
Extraction of the present invention is extract at low temperature; Water, ethanol, methanol or their solution backflow, dipping, percolation extract.
Of the present invention is the medicine of feedstock production treatment tumor with the Herba Solani Nigri, is to be the medicine of multiple cancers such as feedstock production treatment pulmonary carcinoma, bladder cancer, cervical cancer, esophageal carcinoma, gastric cancer, hepatocarcinoma, cancer of pancreas, breast carcinoma with the Herba Solani Nigri.
Below by test example explanation usefulness of the present invention.
One, dries Herba Solani Nigri and lyophilizing Herba Solani Nigri water-soluble extractives and alkaloid relatively
1, Herba Solani Nigri and lyophilizing Herba Solani Nigri are dried in preparation
Herba Solani Nigri is dried in preparation: gets Herba Solani Nigri and removes impurity, and the spray clear water, few profit, cutting is dried.
Preparation lyophilizing Herba Solani Nigri:
1) bright Herba Solani Nigri raw material is removed impurity, is cut into the Herba Solani Nigri section of 2-5cm;
2) with the sabot of Herba Solani Nigri section, sabot weight is 3 ㎏/㎡, and Jiang Wen Zhi – carries out pre-freeze for 40 ℃ gradually, and freezing speed is about 0.5-1.0 ℃/min.
3) to be evacuated to system's absolute pressure be 50Pa to sublimation drying, beginning heat temperature raising to 50 ℃, sublimation drying 24 hours;
4) parsing-desiccation keeps temperature of charge at 45 ℃, makes water content reach 2～3%, gets the lyophilizing Herba Solani Nigri.
2, dry Herba Solani Nigri and lyophilizing Herba Solani Nigri water-soluble extractives, alkaloid content determination
1) adopt " one one of Chinese pharmacopoeia version in 2010, appendix XA method is carried out the water-soluble extractives assay, the result: drying Herba Solani Nigri water extraction content is 13.21%, lyophilizing Herba Solani Nigri water-soluble extractives content is 21.54%.
2) dry the alkaloid of Herba Solani Nigri and lyophilizing Herba Solani Nigri in order to following method mensuration.
Get Herba Solani Nigri and dry medical material and each about 2.0g of lyophilizing Herba Solani Nigri medical material sample fine powder, the accurate title, decide, add dehydrated alcohol 50ml respectively, soaked overnight (12 o'clock), supersound extraction 40min, filter, filtrate is put evaporate to dryness in 50 ℃ of waters bath with thermostatic control, residue adds the 20ml10% acetum makes dissolving, repeatedly extracts with the equal-volume petroleum ether, colourless to petroleum ether layer, water layer changes in the evaporating dish, transfer PH to 9-10 with strong aqua ammonia, put evaporate to dryness in 50 ℃ of waters bath with thermostatic control, residue adds the 20ml1mol ethanol solution hydrochloride makes dissolving, back hydrolysis 1h, take out, put coldly, transfer PH to 9-10 with strong aqua ammonia, use 3 (30ml of chloroform extraction again, 20ml, 10ml), the combined chloroform extract, put evaporate to dryness in 50 ℃ of waters bath with thermostatic control, residue is transferred in the 10ml volumetric flask with chloroform, and the chloroform standardize solution is got 5ml and is transferred in the separatory funnel, the bromocresol green alcoholic solution colour developing that adds 7ml0.1%, collect chloroform layer, it is colourless repeatedly to be extracted to chloroform layer, the combined chloroform layer, put evaporate to dryness in 50 ℃ of waters bath with thermostatic control, residue adds chloroform and repeatedly dissolves on a small quantity and be settled to 10ml, and each surveys absorbance at 414nm place, the calculating alkaloid.
The result: the alkaloid that dries Herba Solani Nigri and lyophilizing Herba Solani Nigri is respectively 0.6% and 2.4%, and lyophilizing Herba Solani Nigri alkaloid is to dry 4 times of Herba Solani Nigri.
Contain multiple biological activity in the Herba Solani Nigri, water-soluble extractives has the immunization of antitumor, antiinflammatory, enhancing body, and water-soluble extractives often is used as the important indicator of estimating quality of medicinal material.Alkaloid in the Herba Solani Nigri is the main effective ingredient of antineoplastic, and its content more high bioactivity is more high.Above experimental result as can be seen lyophilizing Herba Solani Nigri water-soluble extractives content apparently higher than drying Herba Solani Nigri, lyophilizing Herba Solani Nigri alkaloid is apparently higher than drying Herba Solani Nigri, and then strengthening immunity as can be known, the antineoplastic pharmacologically active is higher than solarization lyophilizing Herba Solani Nigri, is used for the treatment of tumor efficiency and is better than the solarization Herba Solani Nigri.
Two, dry Herba Solani Nigri and lyophilizing Herba Solani Nigri to human hepatoma cell strain SMMC-7721, HepG2 and stomach cancer cell line MGC-803 inhibition of proliferation effect test.
1, preparation is dried Herba Solani Nigri and lyophilizing Herba Solani Nigri extract and is got respectively and dry Herba Solani Nigri and the lyophilizing Herba Solani Nigri is pulverized, and respectively decocts with water twice, adds 8 times of amounts of water for the first time, decocted 2 hours, and added 8 times of amounts of water for the second time, decocted 1 hour, merge medicinal liquid, medicinal liquid filters, and gets filtrate, being concentrated into relative density is 1.10(60 ℃) time, add ethanol and make and contain the alcohol amount and reach 75%, precipitation, left standstill 36 hours, and drew supernatant, reclaim ethanol, concentrate, drying is pulverized; Must dry Herba Solani Nigri and lyophilizing Herba Solani Nigri extract.
2, three kinds of cell strains of method MTT method SMMC-7721, HepG2 and MGC-803 are with containing 10% deactivation newborn calf serum, the DMEM culture medium of the penicillin of 100 U/ml and 100 μ g/ml streptomycins is at 37 ℃, cultivate under the 5%CO2 condition, the trophophase cell of taking the logarithm is used for experiment.Getting SMMC-7721, HepG2 and the MGC-803 cell strain 90 μ l that concentration is 2 * 106/ml respectively cultivates in 96 orifice plates, the blank group adds 10 μ lPBS, what the administration group added 10 μ l various dose respectively is subjected to reagent liquid, medicine final concentration (pressing crude drug calculates).If negative control group, negative control group are the medicinal liquids that adds various dose in not celliferous culture fluid.If 6 parallel holes, cultivate 24h after the cell administration, every hole adds 5.0mg/mlMTT solution 20 μ l, behind the vibration mixing, place incubator to continue to hatch 4h, discard the supernatant in each hole, add 100 μ lDMSO stopped reactions again, vibration mixing 10min, treat that purple crystal in its each hole dissolves fully after, with microplate reader at 490 nm places mensuration its light absorption value (A490).Calculate cell inhibitory rate by following formula:
Growth inhibition ratio (%)=(N group OD average-B group OD average)-(T group OD average-TC group OD average)/N group OD average-B group OD average * 100%.
3, the results are shown in Table 1, table 2, table 3.
The suppression ratio result of table 1 couple SMMC-7721
| Suppression ratio (%) | 10（μg/ml） | 30（μg/ml） | 50（μg/ml） |
| The lyophilizing Herba Solani Nigri | 62.62 | 81.67 | 92.03 |
| Dry Herba Solani Nigri | 53.18 | 61.54 | 74.59 |
The suppression ratio result of table 2 couple HepG2
| Suppression ratio (%) | 10（μg/ml） | 30（μg/ml） | 50（μg/ml） |
| The lyophilizing Herba Solani Nigri | 60.14 | 68.52 | 76.11 |
| Dry Herba Solani Nigri | 48.15 | 62.53 | 66.06 |
The suppression ratio result of table 3 MGC-803
| Suppression ratio (%) | 10（μg/ml） | 30（μg/ml） | 50（μg/ml） |
| The lyophilizing Herba Solani Nigri | 70.23 | 85.10 | 91.72 |
| Dry Herba Solani Nigri | 51.24 | 70.17 | 80.35 |
The result shows, dries Herba Solani Nigri and lyophilizing Herba Solani Nigri to human hepatoma cell strain SMMC-7721, and HepG2 and stomach cancer cell line MGC-803 propagation all have the work of inhibition, and lyophilizing Herba Solani Nigri curative effect is better than dries Herba Solani Nigri.
The specific embodiment
Be the medicine of feedstock production treatment tumor with the lyophilizing herb of Herba Solani Nigri.
Be the medicine of feedstock production treatment tumor with the lyophilizing Fructus Solani Nigri.
The lyophilizing herb of Herba Solani Nigri: bright herb of Herba Solani Nigri, clean roguing, be cut into segment, sabot weight is 5-8 ㎏/㎡, is cooled to-20 ℃～– gradually and carries out pre-freeze for 60 ℃; It is 40～60Pa that sublimation drying is evacuated to system's absolute pressure, beginning heat temperature raising to 30 ℃～50 ℃, sublimation drying 15～24 hours; Parsing-desiccation keeps temperature of charge at 35 ℃～55 ℃, makes water content reach 2～3%, gets the lyophilizing herb of Herba Solani Nigri.
Lyophilizing Fructus Solani Nigri: get Fructus Solani Nigri and wash; Sabot, loading amount are about 5-8Kg/ ㎡; Solidification point control is between-28～-32 ℃; After reaching the control temperature, open vacuum pump immediately and be evacuated to drying chamber pressure 40-50Pa, start heater switch then, promote to rise; The temperature of China's distillation should be controlled below 40 ℃; The temperature of resolution phase should be controlled below 45 ℃, and dry the end gets the lyophilizing Fructus Solani Nigri when temperature of charge is lower than 1 ℃ of heating-up temperature.
Adopting the preparation of Chinese medicine preparation conventional method, is the medicine of feedstock production treatment tumor with the lyophilizing Herba Solani Nigri.
Lyophilizing herb of Herba Solani Nigri or lyophilizing Fructus Solani Nigri are pulverized, made powder.
Lyophilizing herb of Herba Solani Nigri or lyophilizing Fructus Solani Nigri are passed through to extract, and extract adds adjuvant and makes tablet, capsule, granule, pill, but this does not limit protection scope of the present invention.
Water extract at low temperature lyophilizing Herba Solani Nigri.
Water, ethanol, methanol or their solution backflow respectively, dipping, percolation extract the lyophilizing Herba Solani Nigri.
Be the medicine of multiple cancers such as feedstock production treatment pulmonary carcinoma, bladder cancer, cervical cancer, esophageal carcinoma, gastric cancer, hepatocarcinoma, cancer of pancreas, breast carcinoma with the lyophilizing Herba Solani Nigri.
Embodiment 1
Get fresh herb of Herba Solani Nigri, clean roguing, be cut into the segment of length 3-10cm; Sabot, sabot weight are 5 ㎏/㎡, are cooled to-20 ℃ gradually and carry out pre-freeze; It is 40Pa that sublimation drying is evacuated to system's absolute pressure, beginning heat temperature raising to 30 ℃, sublimation drying 20 hours; Parsing-desiccation keeps temperature of charge at 35 ℃, makes water content reach 2～3%, gets the lyophilizing herb of Herba Solani Nigri.
Get the lyophilizing herb of Herba Solani Nigri, be crushed to fine powder, be packed as the 3g/ bag, get Herba Solani Nigri powder.Patients with lung cancer is oral, 3 bags of every days, divides clothes 3 times.
Embodiment 2
Get fresh herb of Herba Solani Nigri, clean roguing, be cut into the segment of length 5cm; Sabot, sabot weight are 8 ㎏/㎡, and Jiang Wen Zhi – carries out pre-freeze for 60 ℃ gradually; It is 60Pa that sublimation drying is evacuated to system's absolute pressure, beginning heat temperature raising to 50 ℃, sublimation drying 24 hours; Parsing-desiccation keeps temperature of charge at 55 ℃, makes water content reach 2～3%, gets the lyophilizing herb of Herba Solani Nigri.
Get the lyophilizing herb of Herba Solani Nigri, be crushed to fine powder, be packed as the 10g/ bag, get Herba Solani Nigri powder, liver cancer patient is oral, 3 bags of every days, divides clothes 3 times.
Embodiment 3
Get fresh herb of Herba Solani Nigri, sabot, sabot weight is 6 ㎏/㎡, is cooled to 30 ℃ gradually and carries out pre-freeze; It is 50Pa that sublimation drying is evacuated to system's absolute pressure, beginning heat temperature raising to 50 ℃, sublimation drying 20 hours; Parsing-desiccation keeps temperature of charge at 45 ℃, makes water content reach 2～3%, gets the lyophilizing herb of Herba Solani Nigri.
Get the lyophilizing herb of Herba Solani Nigri, be crushed to coarse powder, add 4 times of water gagings, stir to extract 4 hours at 20 ℃, totally 2 times, merge extractive liquid, filters, and is centrifugal, the centrifugal liquid freeze drying box of packing into, lyophilizing.Get the lyophilizing Herba Solani Nigri extract.
Get extract, add an amount of dextrin, granulate, the reduced vacuum drying, granulate is packed as the 10g/ bag, gets lyophilizing Herba Solani Nigri granule, and patients with gastric cancer is oral, 2 bags of every days (being equivalent to lyophilizing Herba Solani Nigri 25g), divides clothes 2 times.
Embodiment 4
Adopt ZG-100 ㎡ type vacuum freeze drier.Getting bright Fructus Solani Nigri (young fruit, crude fruit and mellow fruit mix) washes; Sabot, loading amount are about 7Kg/ ㎡; Solidification point control is at-28 ℃; After reaching the control temperature, open vacuum pump immediately and be evacuated to below the drying chamber pressure 45Pa, start heater switch then, promote to rise; The temperature of China's distillation should be controlled below 40 ℃; The temperature of resolution phase should be controlled below 45 ℃, and dry the end gets the lyophilizing Fructus Solani Nigri when temperature of charge is lower than 5 ℃ of heating-up temperatures.
Get lyophilizing lyophilizing Fructus Solani Nigri and be crushed to coarse powder, add 6 times of water gagings, stir to extract 4 hours at 20 ℃, totally 2 times, merge extractive liquid, filters, and is centrifugal, the centrifugal liquid freeze drying box of packing into, lyophilizing.Get lyophilizing Fructus Solani Nigri extract.
Get lyophilizing Fructus Solani Nigri extract, pulverize, wet granulation, the reduced vacuum drying, granulate, the adding Pulvis Talci is an amount of, and tabletting gets lyophilizing Herba Solani Nigri chankings, and the cancer patient is oral, 18 of every days (being equivalent to lyophilizing Fructus Solani Nigri 40g), divides clothes 3 times.
Embodiment 5
Adopt ZG-100 ㎡ type vacuum freeze drier.Get bright imperial young fruit, wash; Sabot, loading amount are about 7Kg/ ㎡; Solidification point control is at-28 ℃; After reaching the control temperature, open vacuum pump immediately and be evacuated to below the drying chamber pressure 45Pa, start heater switch then, promote to rise; The temperature of China's distillation should be controlled below 40 ℃; The temperature of resolution phase should be controlled below 45 ℃, and dry the end gets the lyophilizing Fructus Solani Nigri when temperature of charge is lower than 1 ℃ of heating-up temperature.
Get lyophilizing lyophilizing Fructus Solani Nigri and be crushed to coarse powder, add 6 times of water gagings, stir to extract 4 hours at 20 ℃, totally 2 times, merge extractive liquid, filters, and is centrifugal, the centrifugal liquid freeze drying box of packing into, lyophilizing.Get lyophilizing Fructus Solani Nigri extract.
Get lyophilizing Fructus Solani Nigri extract, pulverize, wet granulation, the reduced vacuum drying, granulate, the adding Pulvis Talci is an amount of, and tabletting gets lyophilizing Herba Solani Nigri chankings, and the cancer patient is oral, 18 of every days (being equivalent to lyophilizing Fructus Solani Nigri 40g), divides clothes 3 times.
Embodiment 6
Adopt ZG-100 ㎡ type vacuum freeze drier.Get bright Herba Solani Nigri immaturity and ripe the mixing really, wash; Sabot, loading amount are about 7Kg/ ㎡; Solidification point control is at-28 ℃; After reaching the control temperature, open vacuum pump immediately and be evacuated to below the drying chamber pressure 45Pa, start heater switch then, promote to rise; The temperature of China's distillation should be controlled below 40 ℃; The temperature of resolution phase should be controlled below 45 ℃, and dry the end gets the lyophilizing Fructus Solani Nigri when temperature of charge is lower than 1 ℃ of heating-up temperature.
Get lyophilizing lyophilizing Fructus Solani Nigri and be crushed to coarse powder, add 6 times of water gagings, stir to extract 4 hours at 20 ℃, totally 2 times, merge extractive liquid, filters, and is centrifugal, the centrifugal liquid freeze drying box of packing into, lyophilizing.Get lyophilizing Fructus Solani Nigri extract.
Get lyophilizing Fructus Solani Nigri extract, pulverize, wet granulation, the reduced vacuum drying, granulate, the adding Pulvis Talci is an amount of, and tabletting gets lyophilizing Herba Solani Nigri chankings, and the cancer patient is oral, 18 of every days (being equivalent to lyophilizing Fructus Solani Nigri 40g), divides clothes 3 times.
Embodiment 7
Adopt ZG-100 ㎡ type vacuum freeze drier.Get bright Herba Solani Nigri mellow fruit, wash; Sabot, loading amount are about 7Kg/ ㎡; Solidification point control is at-28 ℃; After reaching the control temperature, open vacuum pump immediately and be evacuated to below the drying chamber pressure 45Pa, start heater switch then, promote to rise; The temperature of China's distillation should be controlled below 40 ℃; The temperature of resolution phase should be controlled below 45 ℃, and dry the end gets the lyophilizing Fructus Solani Nigri when temperature of charge is lower than 1 ℃ of heating-up temperature.
Get lyophilizing lyophilizing Fructus Solani Nigri and be crushed to coarse powder, add 6 times of water gagings, stir to extract 4 hours at 20 ℃, totally 2 times, merge extractive liquid, filters, and is centrifugal, the centrifugal liquid freeze drying box of packing into, lyophilizing.Get lyophilizing Fructus Solani Nigri extract.
Get lyophilizing Fructus Solani Nigri extract, pulverize, wet granulation, the reduced vacuum drying, granulate, the adding Pulvis Talci is an amount of, and tabletting gets lyophilizing Herba Solani Nigri chankings, and the cancer patient is oral, 18 of every days (being equivalent to lyophilizing Fructus Solani Nigri 40g), divides clothes 3 times.
Claims (6)
1. be the medicine of feedstock production treatment tumor with the lyophilizing Herba Solani Nigri.
According to claim 1 be the medicine of feedstock production treatment tumor with the lyophilizing Herba Solani Nigri, be characterised in that with lyophilizing herb of Herba Solani Nigri or lyophilizing Fructus Solani Nigri to be the medicine of feedstock production treatment tumor.
According to claim 1 or claim 2 be the medicine of feedstock production treatment tumor with the lyophilizing Herba Solani Nigri, be characterised in that with the lyophilizing Fructus Solani Nigri to be the medicine of feedstock production treatment tumor.
According to claim 1 or claim 2 be the medicine of feedstock production treatment tumor with the lyophilizing Herba Solani Nigri, be characterised in that with following technology lyophilizing herb of Herba Solani Nigri: bright herb of Herba Solani Nigri, segment is cut in clean roguing; Sabot, sabot weight are 5-8 ㎏/㎡, are cooled to-20 ℃～– gradually and carry out pre-freeze for 60 ℃; It is 40～60Pa that sublimation drying is evacuated to system's absolute pressure, beginning heat temperature raising to 30 ℃～60 ℃, sublimation drying 15～24 hours; Parsing-desiccation keeps temperature of charge at 35 ℃～65 ℃, makes water content reach 2～3%, gets the lyophilizing herb of Herba Solani Nigri.
According to claim 1 or claim 2 or claim 3 be the medicine of feedstock production treatment tumor with the lyophilizing Herba Solani Nigri, be characterised in that with following technology lyophilizing Fructus Solani Nigri: get Fructus Solani Nigri and wash; Sabot, loading amount are about 5-8Kg/ ㎡; Solidification point control is between-28～-32 ℃; After reaching the control temperature, open vacuum pump immediately and be evacuated to drying chamber pressure 40-50Pa, start heater switch then, promote distillation; The temperature of distillation should be controlled below 40 ℃; The temperature of resolution phase should be controlled below 45 ℃, when temperature of charge and heating-up temperature near the time dry the end, get the lyophilizing Fructus Solani Nigri.
According to claim 1 or claim 2 or claim 3 be the medicine of feedstock production treatment tumor with the lyophilizing Herba Solani Nigri, be characterised in that the lyophilizing Herba Solani Nigri replaces with bright herb of Herba Solani Nigri or bright Fructus Solani Nigri, its consumption press dry product and is converted.
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| Application Number | Priority Date | Filing Date | Title |
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| CN2013100657733A CN103181988A (en) | 2013-03-03 | 2013-03-03 | Freeze-dried black nightshade for treating cancers |
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| CN2013100657733A CN103181988A (en) | 2013-03-03 | 2013-03-03 | Freeze-dried black nightshade for treating cancers |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107260685A (en) * | 2017-06-02 | 2017-10-20 | 神威药业集团有限公司 | A kind of preparation method of Solanum nigrum granule |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1061531A (en) * | 1990-11-10 | 1992-06-03 | 蚌埠市雄蜂实业有限公司 | The new method that a kind of Chinese crude drug is concocted |
| CN1255342A (en) * | 1998-11-26 | 2000-06-07 | 沈阳药科大学 | Process for preparing black nightshade extract as anticancer medicine |
-
2013
- 2013-03-03 CN CN2013100657733A patent/CN103181988A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1061531A (en) * | 1990-11-10 | 1992-06-03 | 蚌埠市雄蜂实业有限公司 | The new method that a kind of Chinese crude drug is concocted |
| CN1255342A (en) * | 1998-11-26 | 2000-06-07 | 沈阳药科大学 | Process for preparing black nightshade extract as anticancer medicine |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107260685A (en) * | 2017-06-02 | 2017-10-20 | 神威药业集团有限公司 | A kind of preparation method of Solanum nigrum granule |
| CN107260685B (en) * | 2017-06-02 | 2020-11-24 | 神威药业集团有限公司 | Preparation method of solanum nigrum fruit formula granules |
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Application publication date: 20130703 |