CN103181982A - Medicine for treating cancer - Google Patents
Medicine for treating cancer Download PDFInfo
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- CN103181982A CN103181982A CN2013100657678A CN201310065767A CN103181982A CN 103181982 A CN103181982 A CN 103181982A CN 2013100657678 A CN2013100657678 A CN 2013100657678A CN 201310065767 A CN201310065767 A CN 201310065767A CN 103181982 A CN103181982 A CN 103181982A
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Abstract
The invention relates to a medicine for treating tumor with high activity by taking one of lyophilized solanum lyratum, lyophilized pseudocapsicum var. diflorum, lyophilized bittersweet and lyophilized nightshade as a raw material. The medicine is characterized by taking the lyophilized herb or the lyophilized fruit as the raw material to prepare the medicine for treating tumor, preferably the lyophilized fruit. The medicine which is prepared with lyophilized solanum lyratum, lyophilized pseudocapsicum var. diflorum, lyophilized bittersweet or lyophilized nightshade has better anti-cancer effect than that prepared by the dried material.
Description
Technical field
The present invention relates to medicine, particularly a kind of a kind of medicine for the treatment of cancer of cancer for the treatment of belongs to medical technical field.
Background technology
Herba Solani Lyrati is the dry herb of plant of Solanaceae Herba Solani Lyrati SolanumlyratumThunb., and the beginning is stated from Shennong's Herbal, classifies as top gradely, and most books on Chinese herbal medicine are all on the books thereafter." Chinese pharmacopoeia version in 1977 is recorded this medicine, and many provincial standards are all recorded as " Beijing Chinese crude drug standard ", " Guangxi Chinese crude drug standard " (version in 1996), " Guizhou Chinese crude drug standard ", " Henan Chinese crude drug standard " (version in 1993).Herba Solani Lyrati has heat-clearing and toxic substances removing, and effects such as detumescence are used for the treatment of diseases such as malaria, yellow cellulitis, edema, gonorrhea, rheumatic arthritis, cholecystitis, cancer, cervical erosion, leucorrhea abnormal, treatment poison.Modern pharmacological research shows that Herba Solani Lyrati has effects such as antitumor, antiinflammatory, antifungal.The clinical multiple cancers such as pulmonary carcinoma, bladder cancer, cervical cancer, esophageal carcinoma, gastric cancer, hepatocarcinoma, cancer of pancreas, breast carcinoma that are used for the treatment of more.Especially pulmonary carcinoma, cervical cancer there is obvious curative effects.
Corallium Japonicum Kishinouye bean (Solanum pseudo-capsicumvar diflorum (Vell.) Bitter) is Solanaceae (Solanaceae) Solanum (Solanum L) plant, have another name called: see Fructus Jujubae, Corallium Japonicum Kishinouye cherry, Jerusalem cherry, beautiful Corallium Japonicum Kishinouye, Corallium Japonicum Kishinouye, its root is used as medicine, warm in nature, the little hardship of salty in the mouth, tool pain relieving effect is used for lumbar muscle strain.Clinically do not see the report that is used for the treatment of cancer.
Herba Solani Nigri Solanum nigrumL. is Solanaceae (Solanaceace) Solanum annual herb plant.Another name Radix vitis romanetii, crow eyes grass, Herba Sonchi Oleracei, bitter certain herbaceous plants with big flowers.Herb of Herba Solani Nigri is used as medicine, and bitter in the mouth, cold in nature, little sweet, slightly poisonous is returned lung, stomach, urinary bladder channel, and the effect of heat-clearing and toxic substances removing, promoting blood circulation to remove blood stasis, inducing diuresis to remove edema, eliminating phlegm and stopping cough is arranged.Be used for sore and toxic, osteopatia sprain, chronic tracheitis, acute nephritis, skin eczema, dysuria, chronic senile tracheitis, leukorrhagia, prostatitis, diseases such as dysentery.Herba Solani Nigri and compound preparation thereof are widely used in treating all kinds of tumors clinically, are classified as one of ten big antitumor Chinese herbal medicine by Chinese medicine, and its antitumor action has caused the attention of research worker, and become the research focus of Chinese scholars.
Herba Solani Lyrati is the dry aerial parts of the blue or green Qi of plant of Solanaceae (Solanum septemlobum Bunge).Another name has: Yang Quan, blue or green Qi, Herba Saginae Japonicae, Herba Solani Septemlobi, wild Fructus Lycii, Radix solani coagulantis, child's fist, red ear pendant etc.Tap late summer, removes impurity, dries.The property hardship, cold, return liver, the stomach warp.The effect of tool heat-clearing and toxic substances removing.Be used for hot carbuncle toxin, laryngopharynx swelling and pain, itchy skin eruption.
We find that lyophilizing Herba Solani Lyrati, lyophilizing Corallium Japonicum Kishinouye bean, lyophilizing Herba Solani Lyrati, lyophilizing Herba Solani Nigri active anticancer are higher than Herba Solani Lyrati, Corallium Japonicum Kishinouye bean, Herba Solani Lyrati, the Herba Solani Nigri of drying through overtesting.
Summary of the invention
The objective of the invention is with a kind of in lyophilizing Herba Solani Lyrati, lyophilizing Corallium Japonicum Kishinouye bean, lyophilizing Herba Solani Lyrati, the lyophilizing Herba Solani Nigri is the medicine of the highly active treatment tumor of feedstock production.
A kind of medicine for the treatment of cancer is characterised in that with a kind of in lyophilizing Herba Solani Lyrati, lyophilizing Corallium Japonicum Kishinouye bean, lyophilizing Herba Solani Lyrati, the lyophilizing Herba Solani Nigri be feedstock production.
A kind of medicine for the treatment of cancer of the present invention is characterised in that with a kind of in lyophilizing Herba Solani Lyrati fruit, lyophilizing Corallium Japonicum Kishinouye bean fruit, lyophilizing Herba Solani Lyrati fruit, the lyophilizing Fructus Solani Nigri be feedstock production.
Lyophilizing of the present invention is characterised in that and uses following technology:
Bright herb is cleaned roguing, is cut into segment; Sabot, sabot weight are 5-8 ㎏/㎡, are cooled to-20 ℃～– gradually and carry out pre-freeze for 60 ℃; It is 40～60Pa that sublimation drying is evacuated to system's absolute pressure, beginning heat temperature raising to 30 ℃～60 ℃, sublimation drying 15～24 hours; Parsing-desiccation keeps temperature of charge at 35 ℃～65 ℃, makes water content reach 2～3%, gets the lyophilizing medical material.
The purpose of lyophilizing Herba Solani Lyrati, Corallium Japonicum Kishinouye bean, Herba Solani Lyrati, Herba Solani Nigri is to solve technical problems such as to dry loss of effective components bigger, and active anticancer is low.Directly can reduce cost with bright product preparation medicine of the present invention in their harvest seasons.Accordingly, lyophilizing Herba Solani Lyrati of the present invention, Corallium Japonicum Kishinouye bean, Herba Solani Lyrati, Herba Solani Nigri can replace with bright product at their harvest seasons dried frozen aquatic products of the present invention, and its consumption is pressed the dry product conversion.
Of the present invention is the medicine of feedstock production treatment tumor with lyophilizing Herba Solani Lyrati, Corallium Japonicum Kishinouye bean, Herba Solani Lyrati, Herba Solani Nigri, is on the basis of existing technology, adopts the Chinese medicine preparation conventional method to be prepared into any pharmaceutically useful preparation.For example, lyophilizing herb or lyophilizing are really pulverized, made powder and take after mixing it with water; By further extraction, extract adds adjuvant and makes tablet, capsule, granule, pill, but this does not limit protection scope of the present invention.
Extraction of the present invention is extract at low temperature; Water, ethanol, methanol or their solution backflow, dipping, percolation extract.
A kind of medicine for the treatment of cancer is the medicine of multiple cancers such as treatment pulmonary carcinoma, bladder cancer, cervical cancer, esophageal carcinoma, gastric cancer, hepatocarcinoma, cancer of pancreas, breast carcinoma.
Below by test example explanation usefulness of the present invention.
One, lyophilizing Herba Solani Lyrati herb, lyophilizing Herba Solani Lyrati fruit, anti-people's lung source adenocarcinoma cell SPCA-1, human hepatoma cell line HepG2 and people's chronic myelogenous leukemia cell K562 test.
1, preparation lyophilizing Herba Solani Lyrati herb, lyophilizing Herba Solani Lyrati berry extract are got lyophilizing Herba Solani Lyrati herb lyophilizing Herba Solani Lyrati respectively and are really pulverized, and add 8 times of water gagings respectively; Stir to extract 4 hours at 20 ℃, totally 2 times, merge extractive liquid, filters, and is centrifugal, the centrifugal liquid freeze drying box of packing into, lyophilizing.Get lyophilizing Herba Solani Lyrati herb, lyophilizing Herba Solani Lyrati berry extract respectively.
2, preparation is dried the Herba Solani Lyrati herb, is dried the Herba Solani Lyrati berry extract and get Herba Solani Lyrati herb, Herba Solani Lyrati leaf, Herba Solani Lyrati respectively and really pulverize, and individually decocts with water twice, adds 8 times of amounts of water for the first time, decocted 2 hours, and added 8 times of amounts of water for the second time, decocted 1 hour, merge medicinal liquid, medicinal liquid filters, and gets filtrate, being concentrated into relative density is 1.10(60 ℃) time, add ethanol and make and contain the alcohol amount and reach 75%, precipitation, left standstill 36 hours, and drew supernatant, reclaim ethanol, concentrate, drying is pulverized; Must dry the Herba Solani Lyrati herb, dry the Herba Solani Lyrati leaf, dry the Herba Solani Lyrati berry extract.
3, tumor cell line people lung source adenocarcinoma cell SPCA-1, human hepatoma cell line HepG2 and people's chronic myelogenous leukemia cell K562 use the RPMI-1649 culture fluid, and 37 ℃, 5%CO
2, relative humidity 100% is cultivated, and attached cell 0.25% trypsinization goes down to posterity.
4, method MTT colorimetric method for determining extract is to the inhibitory action of growth of tumour cell.The trophophase cell of taking the logarithm is mixed with cell suspension with fresh RPMI-1640 culture fluid, 0.8 * 105/ml of 1 * 105/ml of suspension cell attached cell.Suspension cell is inoculated in 96 well culture plates after adding variable concentrations experiment medicine respectively; Attached cell is inoculated in earlier in 96 well culture plates, adds variable concentrations experiment medicine behind every hole 90 μ l 24h respectively.Final volume is that every hole 100 μ l and every group are established 3 parallel holes, establishes 4 groups altogether: be subjected to reagent group T (culture fluid+cell suspension+variable concentrations is subjected to reagent), negative control group N (culture fluid+cell suspension), medicine color matched group TC (culture fluid+variable concentrations is subjected to reagent), blank group B (culture fluid+normal saline).Be subjected to reagent concentration to be followed successively by 10 μ g/ml, 30 μ g/ml, 50 μ g/ml.Put 37 ℃, 5%CO
2After cultivating 72h in the incubator, behind every hole adding MTT solution 10 μ l concussion mixing, continue to cultivate 4h, add SDS90 μ l and stop cultivating, 37 ℃ are spent the night, and shake 10min at micro oscillator under the room temperature then, microplate reader is measured the absorbance (OD value) at 570nm wavelength place, and experiment repeats 3 times.
Calculate growth inhibition ratio as follows:
Growth inhibition ratio (%)=(N group OD average-B group OD average)-(T group OD average-TC group OD average)/N group OD average-B group OD average * 100%.
4, different extracts to the suppression ratio of people's lung source adenocarcinoma cell SPCA-1, human hepatoma cell line HepG2 and people's chronic myelogenous leukemia cell K562 the results are shown in Table 1, table 2, table 3.
The suppression ratio result of table 1 couple SPCA-1
| Suppression ratio (%) | 10（μg/ml） | 30（μg/ml） | 50（μg/ml） |
| The lyophilizing herb extract | 57.28 | 63.49 | 75.21 |
| The lyophilizing berry extract | 77.25 | 81.36 | 87.83 |
| Dry herb extract | 46.27 | 53.43 | 69.15 |
| Dry berry extract | 61.25 | 73.30 | 81.21 |
The suppression ratio result of table 2 couple HepG2
| Suppression ratio (%) | 10（μg/ml） | 30（μg/ml） | 50（μg/ml） |
| The lyophilizing herb extract | 41.96 | 54.19 | 79.54 |
| The lyophilizing berry extract | 73.26 | 82.73 | 89.12 |
| Dry herb extract | 34.29 | 45.61 | 71.81 |
| Dry berry extract | 54.44 | 59.79 | 83.61 |
The suppression ratio result of table 3 couple K562
| Suppression ratio (%) | 10（μg/ml） | 30（μg/ml） | 50（μg/ml） |
| The lyophilizing herb extract | 57.82 | 69.90 | 76.78 |
| The lyophilizing berry extract | 76.59 | 81.68 | 87.96 |
| Dry herb extract | 51.20 | 60.08 | 68.27 |
| Dry berry extract | 68.73 | 73.6 | 78.91 |
The result shows that lyophilizing Herba Solani Lyrati herb, lyophilizing Herba Solani Lyrati fruit all have inhibitory action to the propagation of SPCA-1, HepG2, K562 cell, and its curative effect is better than dries Herba Solani Lyrati herb, Herba Solani Lyrati fruit.
Two, lyophilizing Corallium Japonicum Kishinouye bean Herb, lyophilizing Corallium Japonicum Kishinouye bean leaf, the anti-people's lung of lyophilizing Corallium Japonicum Kishinouye bean fruit source adenocarcinoma cell SPCA-1, human hepatoma cell line HepG2 and people's chronic myelogenous leukemia cell K562 test.
Method is with test one, the results are shown in Table 4, table 5, table 6.
The suppression ratio result of table 4 couple SPCA-1
| Suppression ratio (%) | 10（μg/ml） | 30（μg/ml） | 50（μg/ml） |
| The lyophilizing whole strain extract | 64.54 | 75.27 | 84.46 |
| The lyophilizing leaf extract | 68.32 | 87.97 | 89.89 |
| The lyophilizing berry extract | 86.78 | 89.50 | 90.86 |
| Dry whole strain extract | 53.77 | 64.25 | 80.13 |
| Dry leaf extract | 54.03 | 81.06 | 83.27 |
| Dry berry extract | 68.7 | 85.76 | 88.34 |
The suppression ratio result of table 5 couple HepG2
| Suppression ratio (%) | 10（μg/ml） | 30（μg/ml） | 50（μg/ml） |
| The lyophilizing whole strain extract | 52.78 | 60.81 | 88.69 |
| The lyophilizing leaf extract | 65.24 | 76.38 | 89.91 |
| The lyophilizing berry extract | 87.53 | 90.23 | 91.67 |
| Dry whole strain extract | 40.36 | 52.65 | 83.10 |
| Dry leaf extract | 55.26 | 66.07 | 84.97 |
| Dry berry extract | 62.55 | 70.81 | 90.34 |
The suppression ratio result of table 6 couple K562
| Suppression ratio (%) | 10（μg/ml） | 30（μg/ml） | 50（μg/ml） |
| The lyophilizing whole strain extract | 69.22 | 76.91 | 84.24 |
| The lyophilizing leaf extract | 82.47 | 86.46 | 90.11 |
| The lyophilizing berry extract | 89.61 | 90.88 | 91.74 |
| Dry whole strain extract | 60.56 | 66.98 | 79.90 |
| Dry leaf extract | 77.68 | 78.76 | 80.67 |
| Dry berry extract | 79.34 | 80.43 | 89.23 |
The result shows that lyophilizing Corallium Japonicum Kishinouye bean Herb, lyophilizing Corallium Japonicum Kishinouye bean leaf, lyophilizing Corallium Japonicum Kishinouye bean berry extract all have inhibitory action to the propagation of SPCA-1, HepG2, K562 cell.Its curative effect is better than preparation lyophilizing Corallium Japonicum Kishinouye bean Herb, lyophilizing Corallium Japonicum Kishinouye bean leaf, lyophilizing Corallium Japonicum Kishinouye bean berry extract.
Three, dry Herba Solani Nigri and lyophilizing Herba Solani Nigri to human hepatoma cell strain SMMC-7721, HepG2 and stomach cancer cell line MGC-803 inhibition of proliferation effect test.
Method is with test one, the results are shown in Table 7, table 8, table 9.
The suppression ratio result of table 7 couple SMMC-7721
| Suppression ratio (%) | 10（μg/ml） | 30（μg/ml） | 50（μg/ml） |
| The lyophilizing Herba Solani Nigri | 66.35 | 83.27 | 95.18 |
| Dry Herba Solani Nigri | 55.23 | 63.84 | 79.67 |
The suppression ratio result of table 8 couple HepG2
| Suppression ratio (%) | 10（μg/ml） | 30（μg/ml） | 50（μg/ml） |
| The lyophilizing Herba Solani Nigri | 63.42 | 72.41 | 79.56 |
| Dry Herba Solani Nigri | 51.20 | 66.91 | 70.87 |
The suppression ratio result of table 9 couple MGC-803
| Suppression ratio (%) | 10（μg/ml） | 30（μg/ml） | 50（μg/ml） |
| The lyophilizing Herba Solani Nigri | 73.25 | 89.10 | 90.70 |
| Dry Herba Solani Nigri | 54.39 | 71.52 | 83.75 |
The result shows, dries Herba Solani Nigri and lyophilizing Herba Solani Nigri to human hepatoma cell strain SMMC-7721, and HepG2 and stomach cancer cell line MGC-803 propagation all have the work of inhibition, and lyophilizing Herba Solani Nigri curative effect is better than dries Herba Solani Nigri.
Four, dry Herba Solani Lyrati and lyophilizing Herba Solani Lyrati to human hepatoma cell strain SMMC-7721, HepG2 and stomach cancer cell line MGC-803 inhibition of proliferation effect test.
Method is with test one, the results are shown in Table 10, table 11, table 12.
The suppression ratio result of table 10 couple SMMC-7721
| Suppression ratio (%) | 10（μg/ml） | 30（μg/ml） | 50（μg/ml） |
| The lyophilizing Herba Solani Lyrati | 57.32 | 79.09 | 84.76 |
| Dry Herba Solani Lyrati | 48.73 | 54.80 | 68.99 |
The suppression ratio result of table 11 couple HepG2
| Suppression ratio (%) | 10（μg/ml） | 30（μg/ml） | 50（μg/ml） |
| The lyophilizing Herba Solani Lyrati | 56.31 | 67.47 | 80.25 |
| Dry Herba Solani Lyrati | 45.75 | 57.94 | 68.36 |
The suppression ratio result of table 12 couple MGC-803
| Suppression ratio (%) | 10（μg/ml） | 30（μg/ml） | 50（μg/ml） |
| The lyophilizing Herba Solani Lyrati | 67.18 | 78.64 | 86.70 |
| Dry Herba Solani Lyrati | 46.82 | 62.54 | 79.81 |
The result shows, dries Herba Solani Lyrati and lyophilizing Herba Solani Lyrati to human hepatoma cell strain SMMC-7721, and HepG2 and stomach cancer cell line MGC-803 propagation all have the work of inhibition, and lyophilizing Herba Solani Lyrati curative effect is better than dries Herba Solani Lyrati.
The specific embodiment
Be the medicine of feedstock production treatment tumor with a kind of in lyophilizing Herba Solani Lyrati, lyophilizing Corallium Japonicum Kishinouye bean, lyophilizing Herba Solani Lyrati, the lyophilizing Herba Solani Nigri.
Be the medicine that feedstock production is treated tumor with a kind of in lyophilizing Herba Solani Lyrati fruit, lyophilizing Corallium Japonicum Kishinouye bean fruit, lyophilizing Herba Solani Lyrati fruit, the lyophilizing Fructus Solani Nigri.
With following technology lyophilizing Herba Solani Lyrati, Corallium Japonicum Kishinouye bean, Herba Solani Lyrati, Herba Solani Nigri: bright herb, clean roguing, be cut into segment; Sabot, sabot weight are 5-8 ㎏/㎡, are cooled to-20 ℃～– gradually and carry out pre-freeze for 60 ℃; It is 40～60Pa that sublimation drying is evacuated to system's absolute pressure, beginning heat temperature raising to 30 ℃～60 ℃, sublimation drying 15～24 hours; Parsing-desiccation keeps temperature of charge at 35 ℃～65 ℃, makes water content reach 2～3%, gets the lyophilizing medical material.
With following technology lyophilizing Herba Solani Lyrati fruit, Corallium Japonicum Kishinouye bean fruit, Herba Solani Lyrati fruit, Fructus Solani Nigri: get above-mentioned fresh fruit and wash; Sabot, loading amount are about 5-8Kg/ ㎡; Solidification point control is between-28～-32 ℃; After reaching the control temperature, open vacuum pump immediately and be evacuated to drying chamber pressure 40-50Pa, start heater switch then, promote distillation; The temperature of distillation should be controlled below 40 ℃; The temperature of resolution phase should be controlled below 45 ℃, when temperature of charge and heating-up temperature near the time dry the end, get the lyophilizing fruit.
Lyophilizing Herba Solani Lyrati, Corallium Japonicum Kishinouye bean, Herba Solani Lyrati, Herba Solani Nigri replace with bright product, and its consumption is pressed the dry product conversion.
Be the medicine of feedstock production treatment tumor with lyophilizing Herba Solani Lyrati, Corallium Japonicum Kishinouye bean, Herba Solani Lyrati, Herba Solani Nigri, adopt the Chinese medicine preparation conventional method to be prepared into any pharmaceutically useful preparation.
Be the medicine of feedstock production treatment tumor with lyophilizing Herba Solani Lyrati, Corallium Japonicum Kishinouye bean, Herba Solani Lyrati, Herba Solani Nigri, lyophilizing herb or lyophilizing are really pulverized, make powder and take after mixing it with water;
Be the medicine of feedstock production treatment tumor with lyophilizing Herba Solani Lyrati, Corallium Japonicum Kishinouye bean, Herba Solani Lyrati, Herba Solani Nigri, by extracting, extract adds adjuvant and makes tablet, capsule, granule, pill, but this does not limit protection scope of the present invention.
Water extract at low temperature lyophilizing Herba Solani Lyrati, Corallium Japonicum Kishinouye bean, Herba Solani Lyrati, Herba Solani Nigri.
Chalk water, ethanol, methanol or their solution backflow, dipping, percolation extract lyophilizing Herba Solani Lyrati, Corallium Japonicum Kishinouye bean, Herba Solani Lyrati, Herba Solani Nigri.
Be the medicine of multiple cancers such as feedstock production treatment pulmonary carcinoma, bladder cancer, cervical cancer, esophageal carcinoma, gastric cancer, hepatocarcinoma, cancer of pancreas, breast carcinoma with lyophilizing Herba Solani Lyrati or lyophilizing Corallium Japonicum Kishinouye bean or lyophilizing Herba Solani Lyrati or lyophilizing Herba Solani Nigri.
Embodiment 1
Get fresh Herba Solani Lyrati herb, clean roguing, be cut into the segment of length 3-10cm; Sabot, sabot weight are 5 ㎏/㎡, are cooled to-20 ℃ ℃ gradually and carry out pre-freeze; It is 40Pa that sublimation drying is evacuated to system's absolute pressure, beginning heat temperature raising to 30 ℃, sublimation drying 20 hours; Parsing-desiccation keeps temperature of charge at 35 ℃, makes water content reach 2～3%, gets lyophilizing Herba Solani Lyrati herb.
Get lyophilizing Herba Solani Lyrati herb, be crushed to impalpable powder, be packed as the 3g/ bag, get Herba Solani Lyrati powder.
Embodiment 2
Get fresh Corallium Japonicum Kishinouye bean herb, clean roguing, be cut into the segment of length 5cm; Sabot, sabot weight are 8 ㎏/㎡, and Jiang Wen Zhi – carries out pre-freeze for 60 ℃ gradually; It is 60Pa that sublimation drying is evacuated to system's absolute pressure, beginning heat temperature raising to 60 ℃, sublimation drying 24 hours; Parsing-desiccation keeps temperature of charge at 65 ℃, makes water content reach 2～3%, gets lyophilizing Corallium Japonicum Kishinouye bean.
Get lyophilizing Corallium Japonicum Kishinouye bean, be crushed to fine powder, be packed as the 10g/ bag, get Herba Solani Lyrati powder, liver cancer patient is oral, 3 bags of every days, divides clothes 3 times.
Embodiment 3
Get fresh Herba Solani Lyrati herb, sabot, sabot weight is 6 ㎏/㎡, is cooled to 30 ℃ gradually and carries out pre-freeze; It is 50Pa that sublimation drying is evacuated to system's absolute pressure, beginning heat temperature raising to 50 ℃, sublimation drying 20 hours; Parsing-desiccation keeps temperature of charge at 45 ℃, makes water content reach 2～3%, gets the lyophilizing Herba Solani Lyrati.
Get the lyophilizing Herba Solani Lyrati, be crushed to coarse powder, add 4 times of water gagings, stir to extract 4 hours at 20 ℃, totally 2 times, merge extractive liquid, filters, and is centrifugal, the centrifugal liquid freeze drying box of packing into, lyophilizing.Get lyophilizing Herba Solani Lyrati extract.
Get extract, add an amount of dextrin, granulate, the reduced vacuum drying, granulate is packed as the 10g/ bag, gets lyophilizing Herba Solani Lyrati granule, and patients with gastric cancer is oral, 2 bags of every days (being equivalent to lyophilizing Herba Solani Lyrati 50g), divides clothes 2 times.
Embodiment 4
Adopt ZG-100 ㎡ type vacuum freeze drier.Getting Herba Solani Lyrati really washes; Sabot, loading amount are about 7Kg/ ㎡; Solidification point control is at-28 ℃; After reaching the control temperature, open vacuum pump immediately and be evacuated to below the drying chamber pressure 45Pa, start heater switch then, promote distillation; The temperature of distillation should be controlled below 40 ℃; The temperature of resolution phase should be controlled below 45 ℃, when temperature of charge and heating-up temperature near the time dry the end, get lyophilizing Herba Solani Lyrati fruit.
Get lyophilizing lyophilizing Herba Solani Lyrati and really be crushed to coarse powder, add 6 times of water gagings, stir to extract 4 hours at 20 ℃, totally 2 times, merge extractive liquid, filters, and is centrifugal, the centrifugal liquid freeze drying box of packing into, lyophilizing.Get lyophilizing Herba Solani Lyrati berry extract.
Get lyophilizing Herba Solani Lyrati berry extract, pulverize, wet granulation, the reduced vacuum drying, granulate, tabletting gets lyophilizing Herba Solani Lyrati chankings, and the patient with breast cancer is oral, 18 of every days (being equivalent to lyophilizing Herba Solani Lyrati fruit 40g), divides clothes 3 times.
Embodiment 5
Adopt ZG-100 ㎡ type vacuum freeze drier.Choose fresh Herba Solani Nigri, clean roguing, be cut into the segment of length 3cm, sabot, sabot weight is about 7kg/ ㎡, carries out pre-freeze to-28 ℃ after lowering the temperature gradually 2 hours; Be evacuated to the absolute pressure 40Pa of system then, beginning heat temperature raising to 35 ℃, sublimation drying 15 hours; Keep 35 ℃ of temperature of charge again, when temperature of charge and heating-up temperature near the time dry the end, get the lyophilizing Herba Solani Nigri.
Get the lyophilizing Herba Solani Nigri and be crushed to coarse powder, add 4 times of water gagings, stir to extract 4 hours at 40 ℃, totally 2 times, merge extractive liquid, filters, and is centrifugal, the centrifugal liquid lyophilizing of packing into.Get Herba Solani Nigri extract.
Herba Hedyotidis Diffusae, Radix Clematidis, each 600kg of Rhizoma Imperatae use water extraction 2 times, add 8 times of water gagings for the first time, extracted 2 hours, and added 6 times of water gagings for the second time and extracted merge extractive liquid, 1 hour, filter, concentrate drying, get the Radix Codonopsis Caulis Spatholobi extract, add lyophilizing Herba Solani Nigri extract 300kg, mixing, granulate drying, granulate, tabletting, every 0.4g.Be used for treatment of esophagus cancer, 12 of every days, divide clothes 3 times.Effective percentage 80%.
Embodiment 6
Get lyophilizing Herba Solani Lyrati extract, add an amount of dextrin, granulate, the reduced vacuum drying, granulate is packed as 10g/ bag (being equivalent to lyophilizing Herba Solani Lyrati 50g), gets granule, is used for the treatment of pulmonary carcinoma and suffers from, and divides clothes 2 times 2 bags of every days, and 1 month is a course for the treatment of.
Embodiment 7
Get Herba Solani Lyrati extract, add appropriate amount of starch, granulate, the reduced vacuum drying, granulate incapsulates, and gets lyophilizing Herba Solani Lyrati capsule, and the cervical cancer prostatitis patient is oral, divides clothes 3 times 15 of every days (being equivalent to lyophilizing Herba Solani Nigri 30g), cures in 7 days.
Embodiment 8
Get the lyophilizing Herba Solani Nigri, decoct with water twice, add 8 times of amounts of water for the first time, decocted 2 hours, and added 8 times of amounts of water for the second time, decocted 1 hour, merge medicinal liquid, medicinal liquid filters, and gets filtrate, being concentrated into relative density is 1.10(60 ℃) time, add ethanol and make and contain the alcohol amount and reach 75%, precipitation, left standstill 36 hours, and drew supernatant, reclaim ethanol, concentrate, drying is pulverized; Get Herba Solani Nigri extract.
Get Herba Solani Nigri extract, add appropriate amount of starch and granulate drying, granulate, tabletting.Every is equivalent to Herba Solani Nigri 2g.Be used for the treatment of liver cancer patient, each 3, every day 3 times.1 month was 1 course for the treatment of, and total effective rate is 70.88%.
Claims (6)
1. medicine for the treatment of cancer is characterized in that with a kind of in lyophilizing Herba Solani Lyrati, lyophilizing Corallium Japonicum Kishinouye bean, lyophilizing Herba Solani Lyrati, the lyophilizing Herba Solani Nigri be feedstock production.
2. according to a kind of medicine for the treatment of cancer of claim 1, it is characterized in that with a kind of in lyophilizing Herba Solani Lyrati fruit, lyophilizing Corallium Japonicum Kishinouye bean fruit, lyophilizing Herba Solani Lyrati fruit, the lyophilizing Fructus Solani Nigri be feedstock production.
3. according to a kind of medicine for the treatment of cancer of claim 1 or claim 2, it is characterized in that with following technology lyophilizing: bright medical material, clean roguing, be cut into segment; Sabot, sabot weight are 5-8 ㎏/㎡, are cooled to-20 ℃～– gradually and carry out pre-freeze for 60 ℃; It is 40～60Pa that sublimation drying is evacuated to system's absolute pressure, beginning heat temperature raising to 30 ℃～60 ℃, sublimation drying 15～24 hours; Parsing-desiccation keeps temperature of charge at 35 ℃～65 ℃, makes water content reach 2～3%, gets the lyophilizing medical material.
4. according to a kind of medicine for the treatment of cancer of claim 1 or claim 2, it is characterized in that dried frozen aquatic products replaces with bright product, its consumption is pressed the dry product conversion.
5. according to a kind of medicine for the treatment of cancer of claim 1 or claim 2, it is characterized in that adopting the Chinese medicine preparation conventional method to be prepared into any pharmaceutically useful preparation.
6. according to a kind of medicine for the treatment of cancer of claim 1 or claim 2 or claim 5, it is characterized in that making tablet, capsule, granule, pill.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2013100657678A CN103181982A (en) | 2013-03-03 | 2013-03-03 | Medicine for treating cancer |
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| Application Number | Priority Date | Filing Date | Title |
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| CN2013100657678A CN103181982A (en) | 2013-03-03 | 2013-03-03 | Medicine for treating cancer |
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| CN103181982A true CN103181982A (en) | 2013-07-03 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104083522A (en) * | 2013-04-01 | 2014-10-08 | 张锦超 | Processing method of fresh solanum nigrum fruit, solanum nigrum fruit extract particles and use of solanum nigrum fruit extract particles |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1061531A (en) * | 1990-11-10 | 1992-06-03 | 蚌埠市雄蜂实业有限公司 | The new method that a kind of Chinese crude drug is concocted |
| CN1255342A (en) * | 1998-11-26 | 2000-06-07 | 沈阳药科大学 | Process for preparing black nightshade extract as anticancer medicine |
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2013
- 2013-03-03 CN CN2013100657678A patent/CN103181982A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1061531A (en) * | 1990-11-10 | 1992-06-03 | 蚌埠市雄蜂实业有限公司 | The new method that a kind of Chinese crude drug is concocted |
| CN1255342A (en) * | 1998-11-26 | 2000-06-07 | 沈阳药科大学 | Process for preparing black nightshade extract as anticancer medicine |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104083522A (en) * | 2013-04-01 | 2014-10-08 | 张锦超 | Processing method of fresh solanum nigrum fruit, solanum nigrum fruit extract particles and use of solanum nigrum fruit extract particles |
| CN104083522B (en) * | 2013-04-01 | 2017-11-14 | 张锦超 | The processing method and extract particles and purposes of a kind of black nightshade fresh fruit |
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Application publication date: 20130703 |