CN103181991A - Freeze-dried solanum lytatum thunb fruit for treating cancers - Google Patents
Freeze-dried solanum lytatum thunb fruit for treating cancers Download PDFInfo
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- CN103181991A CN103181991A CN2013100657767A CN201310065776A CN103181991A CN 103181991 A CN103181991 A CN 103181991A CN 2013100657767 A CN2013100657767 A CN 2013100657767A CN 201310065776 A CN201310065776 A CN 201310065776A CN 103181991 A CN103181991 A CN 103181991A
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Abstract
The invention relates to a medicine for treating tumors, which is prepared by utilizing freeze-dried olanum lytatum thunb fruit as a raw material. The medicine is characterized by containing the freeze-dried olanum lytatum thunb fruit, so that the curative effect of the medicine is better than that of medicines prepared by the whole herb of solanum lytatum thunb and sundried olanum lytatum thunb fruit.
Description
Technical field
The present invention relates to medicine, particularly a kind of lyophilizing Herba Solani Lyrati fruit for the treatment of cancer belongs to medical technical field.
Background technology
Herba Solani Lyrati is the dry herb of plant of Solanaceae Herba Solani Lyrati SolanumlyratumThunb., and the beginning is stated from Shennong's Herbal, classifies as top gradely, and most books on Chinese herbal medicine are all on the books thereafter." Chinese pharmacopoeia version in 1977 is recorded this medicine, and many provincial standards are all recorded as " Beijing Chinese crude drug standard ", " Guangxi Chinese crude drug standard " (version in 1996), " Guizhou Chinese crude drug standard ", " Henan Chinese crude drug standard " (version in 1993).Herba Solani Lyrati has heat-clearing and toxic substances removing, and effects such as detumescence are used for the treatment of diseases such as malaria, yellow cellulitis, edema, gonorrhea, rheumatic arthritis, cholecystitis, cancer, cervical erosion, leucorrhea abnormal, treatment poison.
Modern pharmacological research shows that Herba Solani Lyrati has effects such as antitumor, antiinflammatory, antifungal.The clinical multiple cancers such as pulmonary carcinoma, bladder cancer, cervical cancer, esophageal carcinoma, gastric cancer, hepatocarcinoma, cancer of pancreas, breast carcinoma that are used for the treatment of more.Especially pulmonary carcinoma, cervical cancer there is obvious curative effects.
We find through overtesting, and Herba Solani Lyrati fruit active anticancer is higher than the Herba Solani Lyrati herb, lyophilizing Herba Solani Lyrati fruit active anticancer is higher than the Herba Solani Lyrati fruit of drying.
Summary of the invention
The objective of the invention is with Herba Solani Lyrati really is the medicine of the highly active treatment tumor of feedstock production.The object of the present invention is achieved like this, really is the medicine of feedstock production treatment tumor with the lyophilizing Herba Solani Lyrati.
Really be the medicine of feedstock production treatment tumor with the lyophilizing Herba Solani Lyrati, be characterised in that described medicine contains lyophilizing Herba Solani Lyrati fruit.
Of the present invention really is the medicine of feedstock production treatment tumor with the lyophilizing Herba Solani Lyrati, is characterised in that described medicine is really to be prepared by single flavor Herba Solani Lyrati.
Of the present invention really is the medicine of feedstock production treatment tumor with the lyophilizing Herba Solani Lyrati, is characterised in that lyophilizing Herba Solani Lyrati fruit is directly pulverized to make powder and take.
Lyophilizing Herba Solani Lyrati fruit of the present invention is characterised in that with following technology lyophilizing: get Herba Solani Lyrati and really wash; Sabot, loading amount are about 5-8Kg/ ㎡; Solidification point control is between-28～-32 ℃; After reaching the control temperature, open vacuum pump immediately and be evacuated to drying chamber pressure 40-50Pa, start heater switch then, promote distillation; The temperature of distillation should be controlled below 40 ℃; The temperature of resolution phase should be controlled below 45 ℃, when temperature of charge and heating-up temperature near the time dry the end, get lyophilizing Herba Solani Lyrati fruit.
The purpose of lyophilizing Herba Solani Lyrati fruit is to solve technical problems such as to dry loss of effective components bigger, and active anticancer is low.Directly preparing medicine of the present invention with bright Herba Solani Lyrati in the Herba Solani Lyrati harvest season can reduce cost.Accordingly, lyophilizing Herba Solani Lyrati of the present invention can replace with bright Herba Solani Lyrati fruit Herba Solani Lyrati fruit harvest season lyophilizing Herba Solani Lyrati fruit, and its consumption is really converted by extra dry white wine English.
Of the present invention serves as the medicine of fruit feedstock production treatment tumor with the lyophilizing Herba Solani Lyrati, is on the basis of existing technology, adopts the Chinese medicine preparation conventional method to be prepared into any pharmaceutically useful preparation.For example, the lyophilizing Herba Solani Lyrati is really pulverized, made powder and take after mixing it with water; Really by further extracting, extract adds adjuvant and makes tablet, capsule, granule, pill with the lyophilizing Herba Solani Lyrati, but this does not limit protection scope of the present invention.
Extraction of the present invention is extract at low temperature; Water, ethanol, methanol or their solution reflux, extract,, dipping, percolation.
Of the present invention is the medicine of feedstock production treatment tumor with the Herba Solani Lyrati, is really to be the medicine of multiple cancers such as feedstock production treatment pulmonary carcinoma, bladder cancer, cervical cancer, esophageal carcinoma, gastric cancer, hepatocarcinoma, cancer of pancreas, breast carcinoma with Herba Solani Lyrati.
Below by test example explanation usefulness of the present invention.
One, lyophilizing Herba Solani Lyrati herb, the anti-people's lung of Herba Solani Lyrati fruit source adenocarcinoma cell SPCA-1, human hepatoma cell line HepG2 and people's chronic myelogenous leukemia cell K562 test.
1, preparation lyophilizing Herba Solani Lyrati berry extract is got the lyophilizing Herba Solani Lyrati and is really pulverized, and adds 8 times of water gagings respectively; Stir to extract 4 hours at 20 ℃, totally 2 times, merge extractive liquid, filters, and is centrifugal, the centrifugal liquid freeze drying box of packing into, lyophilizing.Get lyophilizing Herba Solani Lyrati berry extract.
2, preparation is dried the Herba Solani Lyrati berry extract and is got and dry the Herba Solani Lyrati Herba Solani Lyrati and really pulverize, and individually decocts with water twice, adds 8 times of amounts of water for the first time, decocted 2 hours, and added 8 times of amounts of water for the second time, decocted 1 hour, merge medicinal liquid, medicinal liquid filters, and gets filtrate, being concentrated into relative density is 1.10(60 ℃) time, add ethanol and make and contain the alcohol amount and reach 75%, precipitation, left standstill 36 hours, and drew supernatant, reclaim ethanol, concentrate, drying is pulverized; Must dry the Herba Solani Lyrati berry extract.
3, tumor cell line people lung source adenocarcinoma cell SPCA-1, human hepatoma cell line HepG2 and people's chronic myelogenous leukemia cell K562 use the RPMI-1649 culture fluid, and 37 ℃, 5%CO
2, relative humidity 100% is cultivated, and attached cell 0.25% trypsinization goes down to posterity.
4, method MTT colorimetric method for determining extract is to the inhibitory action of growth of tumour cell.The trophophase cell of taking the logarithm is mixed with cell suspension with fresh RPMI-1640 culture fluid, 0.8 * 105/ml of 1 * 105/ml of suspension cell attached cell.Suspension cell is inoculated in 96 well culture plates after adding variable concentrations experiment medicine respectively; Attached cell is inoculated in earlier in 96 well culture plates, adds variable concentrations experiment medicine behind every hole 90 μ l 24h respectively.Final volume is that every hole 100 μ l and every group are established 3 parallel holes, establishes 4 groups altogether: be subjected to reagent group T (culture fluid+cell suspension+variable concentrations is subjected to reagent), negative control group N (culture fluid+cell suspension), medicine color matched group TC (culture fluid+variable concentrations is subjected to reagent), blank group B (culture fluid+normal saline).Be subjected to reagent concentration to be followed successively by 10 μ g/ml, 30 μ g/ml, 50 μ g/ml.Put 37 ℃, 5%CO
2After cultivating 72h in the incubator, behind every hole adding MTT solution 10 μ l concussion mixing, continue to cultivate 4h, add SDS90 μ l and stop cultivating, 37 ℃ are spent the night, and shake 10min at micro oscillator under the room temperature then, microplate reader is measured the absorbance (OD value) at 570nm wavelength place, and experiment repeats 3 times.
Calculate growth inhibition ratio as follows:
Growth inhibition ratio (%)=(N group OD average-B group OD average)-(T group OD average-TC group OD average)/N group OD average-B group OD average * 100%.
4, different extracts to the suppression ratio of people's lung source adenocarcinoma cell SPCA-1, human hepatoma cell line HepG2 and people's chronic myelogenous leukemia cell K562 the results are shown in Table 1, table 2, table 3.
The suppression ratio result of table 1 couple SPCA-1
| Suppression ratio (%) | 10（μg/ml） | 30（μg/ml） | 50（μg/ml） |
| The lyophilizing berry extract | 77.25 | 81.36 | 87.83 |
| Dry berry extract | 61.25 | 73.30 | 81.21 |
The suppression ratio result of table 2 couple HepG2
| Suppression ratio (%) | 10（μg/ml） | 30（μg/ml） | 50（μg/ml） |
| The lyophilizing berry extract | 73.26 | 82.73 | 89.12 |
| Dry berry extract | 54.44 | 59.79 | 83.61 |
The suppression ratio result of table 3 couple K562
| Suppression ratio (%) | 10（μg/ml） | 30（μg/ml） | 50（μg/ml） |
| The lyophilizing berry extract | 76.59 | 81.68 | 87.96 |
| Dry berry extract | 68.73 | 73.6 | 78.91 |
The Herba Solani Lyrati of table lyophilizing as a result fruit, dry the Herba Solani Lyrati berry extract propagation of SPCA-1, HepG2, K562 cell is all had inhibitory action, its curative effect lyophilizing Herba Solani Lyrati really is better than and dries the Herba Solani Lyrati fruit.
The specific embodiment
The medicine that really adds other raw material of Chinese medicine preparation treatment tumors with the lyophilizing Herba Solani Lyrati.
Really be the medicine of feedstock production treatment tumor with single flavor lyophilizing Herba Solani Lyrati.
Make powder with direct pulverizing of lyophilizing Herba Solani Lyrati fruit, be used for the treatment of tumor.
With following technology lyophilizing Herba Solani Lyrati really: get Herba Solani Lyrati and really wash; Sabot, loading amount are about 5-8Kg/ ㎡; Solidification point control is between-28～-32 ℃; After reaching the control temperature, open vacuum pump immediately and be evacuated to drying chamber pressure 40-50Pa, start heater switch then, promote distillation; The temperature of distillation should be controlled below 40 ℃; The temperature of resolution phase should be controlled below 45 ℃, and dry end the when temperature of charge is lower than 2 ℃ of heating-up temperatures gets lyophilizing Herba Solani Lyrati fruit.
The lyophilizing Herba Solani Lyrati really replaces with bright Herba Solani Lyrati fruit, and its consumption is really converted by extra dry white wine English, the medicine of preparation treatment tumor.
Adopting the Chinese medicine preparation conventional method, really is that feedstock production becomes any pharmaceutically useful preparation with the lyophilizing Herba Solani Lyrati.
The lyophilizing Herba Solani Lyrati is really pulverized, made powder and take after mixing it with water;
Really by extracting, extract adds adjuvant and makes tablet, capsule, granule, pill with the lyophilizing Herba Solani Lyrati.
Extract at low temperature lyophilizing Herba Solani Lyrati fruit.
Water, ethanol, methanol or their solution backflow respectively, dipping, percolation extract lyophilizing Herba Solani Lyrati fruit.
Really be the medicine of multiple cancers such as feedstock production treatment pulmonary carcinoma, bladder cancer, cervical cancer, esophageal carcinoma, gastric cancer, hepatocarcinoma, cancer of pancreas, breast carcinoma with Herba Solani Lyrati.
Embodiment 1
Get fresh Herba Solani Lyrati fruit (young fruit, crude fruit, mellow fruit mix), clean roguing; Sabot, sabot weight are 5 ㎏/㎡, are cooled to-20 ℃ ℃ gradually and carry out pre-freeze; It is 40Pa that sublimation drying is evacuated to system's absolute pressure, beginning heat temperature raising to 30 ℃ ℃, sublimation drying 20 hours; Parsing-desiccation keeps temperature of charge at 35 ℃, makes water content reach 2～3%, gets lyophilizing Herba Solani Lyrati fruit.
Get lyophilizing Herba Solani Lyrati fruit, be crushed to impalpable powder, be packed as the 3g/ bag, get Herba Solani Lyrati powder.
Embodiment 2
Get fresh Herba Solani Lyrati fruit young fruit, clean roguing; Sabot, sabot weight are 8 ㎏/㎡, and Jiang Wen Zhi – carries out pre-freeze for 60 ℃ gradually; It is 60Pa that sublimation drying is evacuated to system's absolute pressure, beginning heat temperature raising to 60 ℃, sublimation drying 24 hours; Parsing-desiccation keeps temperature of charge at 65 ℃, the dry end when temperature of charge is lower than 1 ℃ of heating-up temperature, and, get lyophilizing Herba Solani Lyrati fruit.
Get lyophilizing Herba Solani Lyrati fruit, be crushed to fine powder, be packed as the 10g/ bag, get Herba Solani Lyrati powder, liver cancer patient is oral, 3 bags of every days, divides clothes 3 times.Curative effect is obvious.
Embodiment 3
Get fresh immaturity Herba Solani Lyrati fruit, sabot, sabot weight is 6 ㎏/㎡, is cooled to 30 ℃ gradually and carries out pre-freeze; It is 50Pa that sublimation drying is evacuated to system's absolute pressure, beginning heat temperature raising to 50 ℃, sublimation drying 20 hours; Parsing-desiccation keeps temperature of charge at 45 ℃, makes water content reach 2～3%, gets lyophilizing Herba Solani Lyrati fruit.
Get lyophilizing Herba Solani Lyrati fruit, be crushed to coarse powder, adds 4 times of water gagings, stir at 20 ℃ and extracted 4 hours, totally 2 times, merge extractive liquid,, filtration, centrifugal, the centrifugal liquid freeze drying box of packing into, lyophilizing.Get lyophilizing Herba Solani Lyrati berry extract.
Get extract, add an amount of dextrin, granulate, the reduced vacuum drying, granulate is packed as the 10g/ bag, gets lyophilizing Herba Solani Lyrati granule, and patients with gastric cancer is oral, 2 bags of every days (being equivalent to lyophilizing Herba Solani Lyrati leaf 50g), divides clothes 2 times.Curative effect is obvious.
Embodiment 4
Adopt ZG-100 ㎡ type vacuum freeze drier, get ripe Herba Solani Lyrati and really wash; Sabot, loading amount are about 7Kg/ ㎡; Solidification point control is at-28 ℃; After reaching the control temperature, open vacuum pump immediately and be evacuated to below the drying chamber pressure 45Pa, start heater switch then, promote to rise; The temperature of China's distillation should be controlled below 40 ℃; The temperature of resolution phase should be controlled below 45 ℃, and dry end the when temperature of charge is lower than 5 ℃ of heating-up temperatures gets lyophilizing Herba Solani Lyrati fruit.
Get lyophilizing lyophilizing Herba Solani Lyrati and really be crushed to coarse powder, add 6 times of water gagings, stir to extract 4 hours at 20 ℃, totally 2 times, merge extractive liquid, filters, and is centrifugal, the centrifugal liquid freeze drying box of packing into, lyophilizing.Get lyophilizing Herba Solani Lyrati berry extract.
Get lyophilizing Herba Solani Lyrati berry extract, pulverize, wet granulation, the reduced vacuum drying, granulate, the adding Pulvis Talci is an amount of, and tabletting gets lyophilizing Herba Solani Lyrati chankings, and the patient with breast cancer is oral, 18 of every days (being equivalent to lyophilizing Herba Solani Lyrati fruit 40g), divides clothes 3 times.Curative effect is obvious.
Embodiment 5
Get the lyophilizing Herba Solani Lyrati and really be crushed to coarse powder, add 4 times of water gagings, stir to extract 4 hours at 40 ℃, totally 2 times, merge extractive liquid, filters, and is centrifugal, the centrifugal liquid lyophilizing of packing into.Get lyophilizing Herba Solani Lyrati berry extract.
Get Radix Codonopsis 1kg, Caulis Spatholobi 0.6kg uses water extraction 2 times, adds 8 times of water gagings for the first time, extracted 2 hours, and added 6 times of water gagings for the second time and extracted merge extractive liquid, 1 hour, filter, concentrate drying, get the Radix Codonopsis Caulis Spatholobi extract, add lyophilizing Herba Solani Lyrati extract 300g, mixing, granulate drying, granulate, it is an amount of to add Pulvis Talci, tabletting, every 0.4g.Be used for the treatment of cervical cancer, 12 of every days, divide clothes 3 times.Curative effect is obvious.
Embodiment 6
Get the lyophilizing Herba Solani Lyrati berry extract that embodiment 5 makes, add an amount of dextrin, granulate, the reduced vacuum drying, granulate is packed as 10g/ bag (being equivalent to lyophilizing Herba Solani Lyrati 50g), gets lyophilizing Rhizoma Dioscoreae granule, is used for the treatment of pulmonary carcinoma and suffers from, and 2 bags of every days, divides clothes 2 times.Curative effect is obvious.
Embodiment 7
Get the lyophilizing lyophilizing Herba Solani Lyrati berry extract that embodiment 5 makes, add an amount of methylcellulose, granulate, the reduced vacuum drying, granulate incapsulates, and gets lyophilizing Herba Solani Lyrati capsule, and patients with gastric cancer is oral, 15 of every days (being equivalent to lyophilizing Herba Solani Lyrati 15g), divides clothes 3 times.Curative effect is obvious.
Embodiment 8
Get the lyophilizing lyophilizing Herba Solani Lyrati berry extract that embodiment 5 makes, add an amount of differential silica gel, pill, the reduced vacuum drying is packed as the 10g/ bag, and liver cancer patient is oral, 2 bags of every days, divides clothes 2 times.Curative effect is obvious.
Claims (6)
1. really be the medicine of feedstock production treatment tumor with the lyophilizing Herba Solani Lyrati, be characterised in that described medicine contains lyophilizing Herba Solani Lyrati fruit.
According to claim 1 really be the medicine of feedstock production treatment tumor with the lyophilizing Herba Solani Lyrati, be characterised in that by single flavor Herba Solani Lyrati really to prepare.
According to claim 1 or claim 2 really be the medicine of feedstock production treatment tumor with the lyophilizing Herba Solani Lyrati, be characterised in that lyophilizing Herba Solani Lyrati fruit is directly pulverized to make powder and take.
According to claim 1 or claim 2 or claim 3 really be the medicine of feedstock production treatment tumor with the lyophilizing Herba Solani Lyrati, be characterised in that with following technology lyophilizing Herba Solani Lyrati really: get Herba Solani Lyrati and really wash; Sabot, loading amount are about 5-8Kg/ ㎡; Solidification point control is between-28～-32 ℃; After reaching the control temperature, open vacuum pump immediately and be evacuated to drying chamber pressure 40-50Pa, start heater switch then, promote distillation; The temperature of distillation should be controlled below 40 ℃; The temperature of resolution phase should be controlled below 45 ℃, when temperature of charge and heating-up temperature near the time dry the end, get lyophilizing Herba Solani Lyrati fruit.
According to claim 1 or claim 2 or claim 3 really be the medicine of feedstock production treatment tumor with the lyophilizing Herba Solani Lyrati, be characterised in that the lyophilizing Herba Solani Lyrati really replaces with bright Herba Solani Lyrati fruit, its consumption is really converted by extra dry white wine English.
According to claim 1 or claim 2 or claim 3 really be the medicine of feedstock production treatment tumor with the lyophilizing Herba Solani Lyrati, be characterised in that and adopt the Chinese medicine preparation conventional method to be prepared into any pharmaceutically useful preparation.
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Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1061531A (en) * | 1990-11-10 | 1992-06-03 | 蚌埠市雄蜂实业有限公司 | The new method that a kind of Chinese crude drug is concocted |
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2013
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Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1061531A (en) * | 1990-11-10 | 1992-06-03 | 蚌埠市雄蜂实业有限公司 | The new method that a kind of Chinese crude drug is concocted |
Non-Patent Citations (1)
| Title |
|---|
| 王文昌等: "白英化学成分及生物活性研究进展", 《广州化工》 * |
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Application publication date: 20130703 |