CN103173385A - Streptomyces and applications thereof to prevention and control of clubroots of crucifers - Google Patents
Streptomyces and applications thereof to prevention and control of clubroots of crucifers Download PDFInfo
- Publication number
- CN103173385A CN103173385A CN2013100497448A CN201310049744A CN103173385A CN 103173385 A CN103173385 A CN 103173385A CN 2013100497448 A CN2013100497448 A CN 2013100497448A CN 201310049744 A CN201310049744 A CN 201310049744A CN 103173385 A CN103173385 A CN 103173385A
- Authority
- CN
- China
- Prior art keywords
- streptomycete
- pests
- streptomyces
- biological
- plant disease
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Images
Landscapes
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses streptomyces and applications thereof to prevention and control of clubroots of crucifers. The streptomyces comprise streptomyces scabiei WS-24926 CCTCC NO: M 2012393 and/or streptomyces cuspidosporus WS-29246 CCTCC NO: M 2012413. The streptomyces can be fermented to produce a fermentation broth with a strong bacteriostasis, and the fermentation broth can be used for preparing a biocontrol suspension agent and a biocontrol slow-release capsule for resisting the clubroots of crucifers. The streptomyces are used for biologically preventing and controlling the clubroots of crucifers.
Description
Technical field
The present invention relates to biological pesticide technical field, specifically refer to a kind of streptomycete and the application in control Cruciferae club root thereof.
Background technology
Cruciferae club root (plasmodiophoa brassicae Woron) is a kind of worldwide soil-borne disease, and pathogenic bacterium are plasmodiophora brassica bacteria, belongs to the low mastigomycetes (also having the scholar to be classified to Acarasiales) that waits of a kind of obligatory parasitism, there is no at present the method artificial culture.The main harm brassicaceous vegetable is as Chinese cabbage, wild cabbage, radish etc.Its germ survives the winter the summer in soil or the barnyard manure that do not become thoroughly decomposed with sporocyst, and is strong to the poor environment resistibility, generally can survive more than 10 years.Carry disease germs from soil and the barnyard manure that do not become thoroughly decomposed in earliest infection source, field.Germ can be carried with dish seedling, earth again and be propagated into without the lesion.Germ is invaded from young root or the root hair of dish seedling, and stimulates root cell to accelerate division, causes the enlargement of root deformity, causes the plant transfusion tissue to be obstructed, the overground part poor growth, and blade is wilted, the severe one butt rot, plant is dead.In recent years, club root in Hubei, the ground high mountain brassicaceous vegetable growing areas such as Yunnan, Sichuan, Guizhou, Zhejiang, Fujian occur serious, become one of principal element that restricts China high mountain Chinese cabbage, wild cabbage, the production of radish anti-season, wherein especially be injured the heaviest with Chinese cabbage, wild cabbage, serious plot even has no harvest, and every mu of loss is more than 3000 yuan.
At present, control Cruciferae club root common method mainly comprises liming regulates soil pH, crop rotation, plantation disease-resistant variety etc., but owing to having soil compaction, deficiency in economic performance, the shortcoming such as anti-accumulating not, and above-mentioned measure is spread not all.The conventional sterilization agent has derosal, thiram, zinc manganese ethylenebisdithiocarbamate etc., but preventive effect is all not obvious, the preventive effect performance is preferably 50% fluazinam SC and the 10% cyazofamid SC combination of Japanese Ishihara Sangyo Kaisha Ltd., but owing to suppressing the reasons such as seed germination and use cost high (every mu nearly 400 yuan), also not yet obtain large scale application.about Cruciferae club root biological pesticide fungi, actinomycetes, all there is certain research report the aspects such as genus bacillus, and obtained certain achievement, as the Japanology person with Chinese cabbage sowing from wheatland, Rapeseed Field, in the soil that adopt back on Chinese cabbage ground and meadow, separate from the Chinese cabbage root and obtain a large amount of endogenetic fungus, screen by pot experiment and obtain 2 strain endogenetic fungus (Heteroconium chaetospira) H4007, M4006, disease refers to be respectively 23, 15, be significantly higher than other and belong to kind of processing and a blank, show growth-promoting functions by seed treatment, there is the investigator to utilize (Phoma glomerata) JCM 9972 effectively to control the generation of club root, the person that also has the foreign study obtains suppressing 3 trichoderma strains (TC32, TC45 and TC63) and 1 streptomycete bacterial strain (S99) of pathogen plasmodiophora by greenhouse and field test, the Korea S researchist is from the Chinese cabbage root portion from obtaining living actinomycetes in 81 strains, by Chinese cabbage club root pot experiment, obtain the interior living actinomycetes that 3 strains have potentiality to be exploited, wherein 2 strains (Microbispora rosea) and (subsp.rosea) preventive effect be respectively 58% and 33%, 1 strain (Streptomyces olivochromogenes) preventive effect and reach 42%, the researchist of China Sichuan agricultural university isolates 1 strain ash red streptomyces (Streptomyces griseoruber) from the Rhizosphere Soil of Sichuan, it reaches 73.69% to Chinese cabbage club root potted plant experiment preventive effect, the researchists such as He Yueqiu are separated to 1 bacillus subtilis (Bacillus subtilis) XF-1 from the Chinese cabbage rhizosphere soil, its control efficacy to club root reaches 85%, and field efficacy reaches 68.6 ~ 84.8%, Changjiang University is used for the club root integrated control with subtilis (Bacillus subtilis) and fixed nitrogen series bacillus (Paenibacillus azotofixans), and preventive effect reaches 85%.
Streptomycete has antibiosis, is developed as biological pesticide but have advantages of also that the field is grown etc. surely.The Environmental Protection Agency EPA was announced the biological pesticide research project of 1 scab streptomycete (Streptomyces acidiscabies) RL-110 for weeding in 2012.the registered streptomycete agricultural chemicals on the regular payroll in the whole world has 6 kinds, the registration countries and regions are respectively Korea S, European Union, Ukraine, Canada, the U.S., be specially (Streptomyces colombiensis) (Korea S), (Streptomyces kasugaensis) (Korea S), (Streptomyces griseoviridis) K61(European Union, Canada, the U.S.), (Streptomyces albus) (Ukraine), (Streptomyces avermitilis) (Ukraine), (Streptomyces lydicus) WYEC 108(Canada, the U.S.), controlling object is mainly fungal disease and comprises leaf diseases and soil-borne disease (wherein S.avermitilis is used for control beetle and tetranychid).But there is no so far, the streptomycete agriculture chemical registration of control Cruciferae club root.
Summary of the invention
One of purpose of the present invention is that a kind of streptomycete with control Cruciferae club root purposes will be provided, and this streptomycete comprises scab streptomycete (Streptomyces scabiei) WS-24926 CCTCC NO:M 2012393 and/or sharp spore streptomycete (Streptomyces cuspidosporus) WS-29246 CCTCC NO:M 2012413.Scab streptomycete provided by the present invention (Streptomyces scabiei) WS-24926 has been preserved in Chinese Typical Representative culture collection center (being called for short CCTCC) on October 10th, 2012; Preservation registration number is CCTCC M 2012393; The preservation place is: China, Wuhan, Wuhan University.Point spore streptomycete (Streptomyces cuspidosporus) WS-29246 has been preserved in Chinese Typical Representative culture collection center (being called for short CCTCC) on October 18th, 2012; Preservation registration number is CCTCC M 2012413, and the preservation place is: China, Wuhan, Wuhan University.
Described scab streptomycete (Streptomyces scabiei) WS-24926 separates from the plant rhizosphere soil of liuyang hunan outer suburbs and obtains.
The result of morphological feature, physio-biochemical characteristics and the 16S rDNA sequential analysis of comprehensive scab streptomycete (Streptomyces scabiei) WS-24926 is accredited as the scab streptomycete with it.Concrete qualification result is as follows:
(1) morphological specificity of thalline
Scab streptomycete (Streptomyces scabiei) WS-24926 bacterial strain is on the ISP-2 solid medium, and bacterium colony is dry, rough, projection is arranged, light gray; Spore is many, chain, and the bat shape, size (long 1.5 μ m-2 μ m, wide 0.6 μ m-0.8 μ m), grey, gas silk oyster white or grey have the spiral fibrillae of spores to produce; Pigment is not obvious; Non-pigment produces on the Cha Shi solid medium, gas silk and sorus white, and the base silk is colourless.The substratum silk is yellow on the asparagine solid medium, gas silk and sorus canescence, and non-pigment produces.
(2) physio-biochemical characteristics
Do not produce melanochrome, can utilize dextrin, polysorbate40, L-arabinose, D-wood sugar, monomethyl succinate, pyruvic acid, glycerol, D-ribose, D-L-alpha-phosphate glycerine.can not utilize: tween 80, alpha-cylodextrin, glycogen, mannosans, D-R, D-Fructose, the D-cellobiose, the D-semi-lactosi, the D-galacturonic acid, PEARLITOL 25C, alpha-D-glucose, maltonic acid, maltose, lactulose, D-MANNOSE, ALANINE, D-alanine, succsinic acid, the L-alanyl-glycine, ALANINE amine, Pidolidone, adenosine, inosine, thymidine, the L-rhamnosyl, uridine, sucrose, saligenin, 6-phosphoric acid-D-Fructose, 6-phosphoric acid-D-Glucose.
(3) 16SrDNA sequential analysis
Scab streptomycete (Streptomyces scabiei) WS-24926 bacterial strain is through round pcr, the DNA sequencer analysis, and the 16SrDNA sequence of this bacterial strain is by 1391 based compositions.With blast program, listed 16SrDNA sequence in the 16SrDNA sequence of scab streptomycete (Streptomyces scabiei) WS-24926 and GenBank is carried out nucleotide homology relatively, result and scab streptomycete (S.scabiei) the ATCC 49173(T that has reported) the 16SrDNA sequence homology of (accession number AB026199) reaches 100%, identifies that it is the scab streptomycete.
Described sharp spore streptomycete (Streptomyces cuspidosporus) WS-29246 separates from the plant rhizosphere soil of outer suburbs, Yuexi, Anhui and obtains.
The result of morphological feature, physio-biochemical characteristics and the 16SrDNA sequential analysis of comprehensive sharp spore streptomycete (Streptomyces cuspidosporus) WS-29246 is accredited as sharp spore streptomycete with it.Concrete qualification result is as follows:
(1) morphological specificity of thalline
Point spore streptomycete (Streptomyces cuspidosporus) WS-29246 is on the ISP-2 solid medium, and bacterium colony is dry, rough, and projection is arranged, and fold is arranged, and oyster white or little yellow have the spirrillum fibrillae of spores, and the 2-5 circle does not wait; Base silk tawny without separating, does not rupture, and is undaform, multi-branched, diameter 0.4 μ m-0.6 μ m.Non-pigment produces on the Cha Shi solid medium, and the bacterium colony smooth surface is not produced spore.Produce lemon yellow pigment on the PDA substratum.
(2) physio-biochemical characteristics
Do not produce melanochrome, utilize D-Glucose, L-arabinose, D-wood sugar, D-Fructose, sucrose, rhamnosyl, raffinose, inositol, PEARLITOL 25C and D-semi-lactosi, lactose, seminose, melibiose, trehalose, D-Maltose, Trisodium Citrate, sodium succinate.
(3) 16SrDNA sequential analysis
point spore streptomycete (Streptomyces cuspidosporus) WS-29246 is through round pcr, DNA sequencer is analyzed, obtain the 16S rDNA sequence of this bacterial strain, then with blast program, listed 16S rDNA sequence in the 16S rDNA sequence of sharp spore streptomycete (Streptomyces cuspidosporus) WS-29246 and GenBank is carried out nucleotide homology relatively, result reaches 100% with the 16S rDNA sequence homology of the sharp spore streptomycete (Streptomyces cuspidosporus) of having reported, identify that it is sharp spore streptomycete (Streptomyces cuspidosporus).
Described scab streptomycete (Streptomyces scabiei) WS-24926 and described sharp spore streptomycete (Streptomyces cuspidosporus) WS-29246 adopt liquid fermentation and culture, and the substratum of its use is as follows:
(1) slant medium: culture medium prescription is as follows: take malt extract: 1.0 ~ 2.0g/L, Zulkovsky starch: 0 ~ 5g/L, yeast extract: 0.4 ~ 1.0g/L, glucose: 0 ~ 2.0g/L, sucrose: 0 ~ 2.0g/L, agar: 1.5 ~ 2.0g/L, pH value 7.0 ~ 8.0 is settled to 1000mL.
(2) seed culture medium: N.F,USP MANNITOL: 10 ~ 20g/L, soy peptone: 5 ~ 20g/L, fish meal: 0 ~ 5.0g/L, soya-bean oil: 0.5 ~ 5.0g/L, K
2HPO
4: 0.15 ~ 0.5g/L, CaCO
3: 0.1 ~ 0.4g/L, pH value 7.0 ~ 8.0 is settled to 1000mL.
(3) fermention medium: glucose: 2 ~ 8g/L, glucose: 2 ~ 8g/L, Zulkovsky starch: 5 ~ 10g/L, soyflour: 0 ~ 10g/L, cotton seed meal: 0 ~ 25g/L, yeast extract: 1 ~ 3g/L, CaCO
3: 2 ~ 5g/L, NaCl:2 ~ 5g/L, pH value 7.0 ~ 8.0 is settled to 1000mL.
Cultivate flow process:
(1) described scab streptomycete (Streptomyces scabiei) WS-24926 and described sharp spore streptomycete (Streptomyces cuspidosporus) WS-29246 bacterial classification are inoculated respectively on the test tube slant substratum, under 28 ± 1 ℃ of conditions of temperature, activation culture 5d;
(2) bacterial classification with activation is seeded in seed culture medium, cultivates 3 ~ 4d in the shaking table of 28 ± 1 ℃ of temperature;
(3) with step 2) in the bacterial classification cultivated be inoculated in fermention medium, cultivate 3 ~ 4d at the condition bottom fermentation of 28 ± 1 ℃ of temperature.
The invention provides the application of described streptomycete in control Cruciferae club root.
The present invention also provides biological and ecological methods to prevent plant disease, pests, and erosion suspension agent of the control Cruciferae club root that described streptomycete is prepared from and preparation method thereof.Described biological and ecological methods to prevent plant disease, pests, and erosion suspension agent is comprised of 10 ~ 80% zymocyte liquid, 0.4 ~ 10% dispersion agent, 0.5 ~ 5% emulsifying agent, 0.4 ~ 10% thickening material, 0.4 ~ 10% sanitas, 0 ~ 3.0% defoamer, 0 ~ 10.0% frostproofer and the water of surplus by weight percentage, wherein, described zymocyte liquid is the bacterium liquid that is obtained by described streptomycete fermentation.
Further, described dispersion agent select in sodium lignosulfonate, calcium lignin sulphonate, naphthalenesulfonic acid-formaldehyde condensate, fatty amide-N methyl taurine salt, alkyl sulfo-succinic acid sodium salt, sodium dibutyl naphthalene sulfonate, sodium lauryl sulphate, polyaryl phenol polyethenoxy ether and edetate choose any one kind of them or several; Described emulsifying agent is to choose any one kind of them in alkylbenzene sulfonate, alkyl alcohol Soxylat A 25-7, alkylphenol polyoxyethylene, styroyl phenol polyethenoxy ether, sorbic ester and polyhydric alcohol fatty acid ester or several; Described thickening material selects xanthan gum, gelatin, gum arabic, melon glue, sodium alginate, Zulkovsky starch, mixed polysaccharide, Xylo-Mucine, sodium hydroxyethlcellulose, polyvinyl alcohol, sodium polyacrylate, poly amic acid, urea, neusilin, silicon-dioxide, diatomite, wilkinite and Attapulgite to choose any one kind of them or several; Described sanitas selects Sodium Benzoate, potassium sorbate and ethyl p-hydroxybenzoate to choose any one kind of them or several; Described frostproofer be in ethylene glycol, propylene glycol, glycerol, glycol ether, triglycol, polyoxyethylene glycol choose any one kind of them or several; Described defoamer is to choose any one kind of them in isooctyl alcohol, primary isoamyl alcohol, stearic acid, lauric acid, polyglycol ether and organosilicon or several.
The preparation method of described biological and ecological methods to prevent plant disease, pests, and erosion suspension agent comprises the following steps:
1) fermented liquid that described streptomycete fermentation is obtained, centrifugal 5 ~ 20min, obtain zymocyte liquid under 4000 ~ 10000r/min condition;
2) mix according to the water of described weight percent with described zymocyte liquid, dispersion agent, emulsifying agent, thickening material, sanitas, defoamer, frostproofer and surplus, obtain mixture;
3) mixture is sheared be uniformly dispersed after, be ground to 90% above raw meal particle size degree and reach below 5 μ m, stir, adjusting pH to 4.0 ~ 5.0 namely obtain the biological and ecological methods to prevent plant disease, pests, and erosion suspension agent.
Experiment shows: biological and ecological methods to prevent plant disease, pests, and erosion suspension agent of the present invention reaches 55 ~ 80% to Cruciferae club root field test prevention effect, and its pot experiment prevention effect is 75 ~ 90%, higher than contrast medicaments such as fluazinam and cyazofamids.
Biological and ecological methods to prevent plant disease, pests, and erosion suspension agent using method of the present invention is: spray method: adopt spray method to carry out soil treatment before transplanting, 50 ~ 200 times of sprayings of described suspension agent dilution are used 1 ~ 2 time with the usage quantity of described suspension agent 1000～2000mL by mu.Root-pouring method: adopt root-pouring method to process during transplanting, with 50 ~ 200 times of suspension agent dilutions, by the suspension 50 ~ 100mL after the described dilution of every strain filling root, mu is 1000 ~ 3000mL with described suspension agent dosage, fills with root whole vegetative period 1 ~ 3 time.
The present invention also provides biological and ecological methods to prevent plant disease, pests, and erosion slow releasing capsule of the control Cruciferae club root that a kind of described streptomycete is prepared from and preparation method thereof.Described biological and ecological methods to prevent plant disease, pests, and erosion slow releasing capsule comprises gelatin or the Xylo-Mucine of the gum arabic of the zymocyte liquid of 1 ~ 3 part of weight, 1 ~ 3 part of weight or seaweed gel, 1 ~ 3 part of weight, and wherein, described zymocyte liquid is the bacterium liquid that is obtained by described streptomycete fermentation.
The preparation method of described biological and ecological methods to prevent plant disease, pests, and erosion slow releasing capsule: comprise the following steps:
1) fermented liquid that first described streptomycete fermentation is obtained, centrifugal 5 ~ 20min, obtain zymocyte liquid under 4000 ~ 10000r/min condition, and the zymocyte liquid of getting 1 ~ 3 part of weight adds reaction vessel;
2) secondly, get the gelatin of 1 ~ 3 part of weight or Xylo-Mucine and add water to be configured to weight percent be after 3 ~ 8% the aqueous solution, to add reaction vessel, and with the pH value to 3 of acetum conditioned reaction thing ~ 5;
3) again, get the gum arabic of 1 ~ 3 part of weight or seaweed gel and add water to be mixed with weight percent be after 2 ~ 8% the aqueous solution, to add and so answer container, continue to stir 10 ~ 30min;
4) then, add formalin to solidify cyst wall in the reaction vessel, continue to stir 10 ~ 30min, namely get the microcapsule aqueous solution;
5) last, will obtain can obtaining the biological and ecological methods to prevent plant disease, pests, and erosion slow releasing capsule after spraying drying after microcapsule aqueous solution centrifugal treating.
Experimental results show that: biological and ecological methods to prevent plant disease, pests, and erosion slow releasing capsule of the present invention is 65 ~ 80% to Chinese cabbage club root field efficacy, and when the field operation technique is Chinese cabbage or wild cabbage transplanting, 2g biological and ecological methods to prevent plant disease, pests, and erosion slow releasing capsule is spread fertilizer over the fields in every cave.
Description of drawings
Fig. 1 is scab streptomycete (Streptomyces scabiei) WS-24926 colonial morphology on the ISP-2 solid medium.
Fig. 2 is the colonial morphology of sharp spore streptomycete (Streptomyces cuspidosporus) WS-29246 on ISP-2.
Embodiment
In order to explain better the present invention, below in conjunction with specific embodiment and Figure of description, streptomycete of the present invention and the application in control Cruciferae club root thereof are described in further detail.
Separation, the screening of embodiment 1 scab streptomycete (Streptomyces scabiei) WS-24926 and sharp spore streptomycete (Streptomyces cuspidosporus) WS-29246 bacterial strain
Soil sampling adopts that conventional method separates, purifying and preservation.We isolate 30000 strain actinomycetes altogether from soil, adopt the active way of following the trail of of lead compound, in conjunction with HPLC-MS natural product compound database, select relative low toxicity, easy cultivation to have 109 strain actinomycetes of Commercialization Trends, by Chinese cabbage club root pot experiment and field test, experimental result sees Table 1.Final definite WS-24926 and WS-29246 are optimum, and its disease index is minimum, proofread and correct preventive effect and are respectively 95% and 79%.
Pot experiment implementation step: after each strains tested actication of culture, adopt the secondary shake flask culture, elementary shake flask fermentation adopts seed culture medium, and culture temperature is 28 ℃, 150rpm, second order fermentation adopts fermention medium, inoculum size is 5 ~ 10%, 28 ℃ of culture condition, rotating speed 150rpm, incubation time 4 ~ 6d, nutrient solution 100mL packs in the every 500mL triangular flask of liquid amount.5 bottles of every strain culturing, every bottled 100mL fermention medium.
Adopt the cave dish to cultivate the healthy seedling of Chinese cabbage, grow to 2 ~ 3 leaf periods for experiment.
Pot experiment adopts the inoculation of bacterium local method, and the Chinese cabbage knee of collecting is shredded with potting soil by 1:100(v/v) mix, flowerpot (ф packs into
On=15cm, ф
Under=10cm, h=13cm) middle for experiment.During transplanting, pour into each bacterial strain shake flask fermentation liquid, every alms bowl 100mL fills with root 1 time every 10d afterwards, and each 100mL fills with root whole vegetative period 4 times altogether.Result demonstration WS-24926 and WS-29246 are best to the prevention effect of Chinese cabbage club root, proofread and correct preventive effect and are respectively 95% and 79%.
The club root grade scale:
0 grade: do not adhere to knee;
1 grade: knee is attached on lateral root, and quantity accounts for 1 ~ 25%;
2 grades: have knee to adhere on main root, on lateral root, knee quantity accounts for more than 25%;
3 grades: knee quantity accounts for 50 ~ 75%; There is knee to adhere on main root;
4 grades: have knee to adhere on main root, knee quantity accounts for the root system more than 75%.
Calculation formula:
The results from pot experiment test of table 1 109 strain actinomycetes to the Cruciferae club root
Annotate: economize " WS-" before strain number.
Embodiment 2 scab streptomycetes (Streptomyces scabiei) WS-24926 and sharp spore streptomycete (Streptomyces cuspidosporus) WS-29246 strain identification
The bacterial strain that screens is through morphological observation, and Physiology and biochemistry experiment and molecular biology identification determine that finally WS-24926 is streptomyces scab streptomycete, and WS-29246 is streptomyces point spore streptomycete.
Scab streptomycete of the present invention (Streptomyces scabiei) WS-24926 and sharp spore streptomycete (Streptomyces cuspidosporus) WS-29246 bacterial strain colonial morphology on the ISP-2 flat board are seen respectively accompanying drawing 1, Fig. 2.
16S rDNA sequential analysis: extract scab streptomycete (Streptomyces scabiei) WS-24926 and sharp spore streptomycete (Streptomyces cuspidosporus) WS-29246 genomic dna, carry out pcr amplification in 50 μ L systems.Adopt primer:
PrimerA：5’-AGAGTTTGATCCTGGCTCAG-3’；
PrimerB：5’-TACGGCTACCTTGTTACGACTT-3’。
Reaction conditions: 94 ℃ of denaturation 5min, 94 ℃ of 1min, 55.5 ℃ of 1min, 72 ℃ of 3min, 35 circulations, 72 ℃ are extended 10min.The 16SrDNA sequencing result utilizes BLAST software to carry out homology search and sequence alignment analysis in GenBank.WS-29246 bacterial strain 16SrRNA gene order GenBank accession number is JQ712974.
By pot experiment and field test, obtain Cruciferae club root biological and ecological methods to prevent plant disease, pests, and erosion streptomycete: obtain scab streptomycete (Streptomyces scabiei) WS-24926 and sharp spore streptomycete (Streptomyces cuspidosporus) WS-29246.The fermented liquid that scab streptomycete (Streptomyces scabiei) WS-24926 and sharp spore streptomycete (Streptomyces cuspidosporus) WS-29246 fermentation is obtained, centrifugal 5 ~ 20min under 4000 ~ 10000r/min condition respectively obtains respectively the zymocyte liquid of the zymocyte liquid of scab streptomycete (Streptomyces scabiei) WS-24926, sharp spore streptomycete (Streptomyces cuspidosporus) WS-29246; Two kinds of zymocyte liquids are mixed to get the zymocyte liquid of scab streptomycete (Streptomyces scabiei) WS-24926 and sharp spore streptomycete (Streptomyces cuspidosporus) WS-29246.Take a sample respectively three kinds of zymocyte liquids standby.
Embodiment 3
getting in the water that the 4kg xanthan gum adds 112kg THE ADIABATIC SHEAR IN mixes well, add afterwards the described standby zymocyte liquid of 800kg (described three kinds of standby zymocyte liquids respectively to be got once, the present embodiment can prepare three kinds of streptomycete biological and ecological methods to prevent plant disease, pests, and erosion suspension agents), the 4kg sodium lignosulfonate, the 20kg alkylbenzene sulfonate, the 30kg sorbic ester, the 20kg potassium sorbate, the 10kg propylene glycol, obtain mixture, dispersed with stirring is even, after grinding by the horizontal sand mill firsts and seconds, through granularity Detection, fineness of materials more than 90% reaches below 5 μ m, stop grinding and stirring, be packaged as finished product, namely obtain the biological and ecological methods to prevent plant disease, pests, and erosion suspension agent.
For high mountain anti-season Chinese cabbage club root, adopt root-pouring method, fill with root 100mL when transplanting, fill with root 1 time every 10d afterwards, fill with root whole vegetative period 3 times.Three kinds of streptomycete biological and ecological methods to prevent plant disease, pests, and erosion suspension agents of the present embodiment and the transnational agricultural chemicals Japan chemical pesticide 50% fluazinam SC of Ishihara Sangyo Kaisha Ltd. of company and 10% cyazofamid SC combination prevent and treat high mountain Chinese cabbage club root field test results and show, streptomycete biological and ecological methods to prevent plant disease, pests, and erosion suspension agent disease index of the present invention is lower than the contrast medicament.Prevention effect is shown in Table 2 in detail.
Table 2 streptomycete biological and ecological methods to prevent plant disease, pests, and erosion suspension agent control Chinese cabbage club root disease index and prevention effect
Annotate: in table 2, " 50% fluazinam SC+50% cyazofamid SC " processes and adopts 150 g/acres of (50% fluazinam SC300mL) even spraying soil of fluazinam, fills with 2 cyazofamids of root (1500 times of liquid of 10% cyazofamid SC dilution) vegetative period.Contrast medicament A is 1,000,000,000 living spores/gram bacillus amyloliquefaciens wettable powder, and contrast medicament B is 50% carbendazol wettable powder, and contrast medicament C is the wettable powder of good fortune more than 50%.Remembered that with column data acceptance of the bid the mean number of different lowercases has significant difference (P＜0.05, Duncan multiple comparisons method (Duncan ' S multiple range test, DMRT)).
Embodiment 4
get the 15kg xanthan gum, the 25kg gelatin, the 30kg gum arabic, 20kg melon glue and 10kg urea add in the water of 611kg THE ADIABATIC SHEAR IN to mix well, add afterwards the standby scab streptomycete of 100kg (Streptomyces scabiei) WS-24926 zymocyte liquid, the 15kg sodium dibutyl naphthalene sulfonate, the 20kg sodium lauryl sulphate, 25kg polyaryl phenol polyethenoxy ether, 25kg alkyl alcohol Soxylat A 25-7, the 4kg ethyl p-hydroxybenzoate, the 30kg glycerol, the 20kg glycol ether, 40kg triglycol and 10kg polyoxyethylene glycol, obtain mixture, dispersed with stirring is even, after grinding by the horizontal sand mill firsts and seconds, through granularity Detection, fineness of materials more than 90% reaches below 5 μ m, stop grinding and stirring, be packaged as finished product, namely obtain the biological and ecological methods to prevent plant disease, pests, and erosion suspension agent.
At Lichuan, Hubei Province high mountain wild cabbage growing area, select club root that relatively serious plot occurs, with 100 times of gained suspension agent dilutions, adopt healthy seedling, root 50 ~ 100mL is filled with in every strain when wild cabbage is transplanted, every mu of ground usage quantity is 3kg, and the field test prevention effect of club-root is reached 66.67%, the heavy 0.89kg of strain.
Embodiment 5
getting 25kg silicon-dioxide and 25kg diatomite adds in 225kg water THE ADIABATIC SHEAR IN to mix well, the zymocyte liquid that adds afterwards 500kg point spore streptomycete (Streptomyces cuspidosporus) WS-29246, the 50kg sodium lignosulfonate, the 40kg calcium lignin sulphonate, the 10kg naphthalenesulfonic acid-formaldehyde condensate, the 5kg sorbic ester, the 20kg Sodium Benzoate, the 10kg potassium sorbate, the 12kg isooctyl alcohol, the 18kg primary isoamyl alcohol, 50kg ethylene glycol, the 10kg propylene glycol, obtain mixture, dispersed with stirring is even, after grinding by the horizontal sand mill firsts and seconds, through granularity Detection, fineness of materials more than 90% reaches below 5 μ m, stop grinding and stirring, be packaged as finished product, namely obtain the biological and ecological methods to prevent plant disease, pests, and erosion suspension agent.
In experimental plot, Hubei Prov. Acdemy of Agricutural Sciences Wuhan, with knee by 1 ︰ 100(v/v) with the potting soil mixing after the dress alms bowl standby, with 50 ~ 100 times of scab streptomycete (Streptomyces scabiei) WS-24926 suspension agent dilutions, root 50 ~ 100mL is filled with in every strain when purple flowering stalk is transplanted, fill with again root after 10d 1 time, 60d " Invest, Then Investigate " preventive effect, streptomycete reaches 90% to the pot experiment prevention effect of purple flowering stalk club root, to plant without poisoning.
Embodiment 6
getting 10kg and 55kg urea xanthan gum adds the water THE ADIABATIC SHEAR IN of 300kg to mix well, the zymocyte liquid mixed solution that adds afterwards the standby scab streptomycete of 395kg (Streptomyces scabiei) WS-24926 and sharp spore streptomycete (Streptomyces cuspidosporus) WS-29246, 30kg polyaryl phenol polyethenoxy ether, the 50kg edetate, the 15kg alkylphenol polyoxyethylene, 25kg styroyl phenol polyethenoxy ether, the 100kg Sodium Benzoate, the 20kg stearic acid, , obtain mixture, dispersed with stirring is even, after grinding by the horizontal sand mill firsts and seconds, through granularity Detection, fineness of materials more than 90% reaches below 5 μ m, stop grinding and stirring, be packaged as finished product, namely obtain the biological and ecological methods to prevent plant disease, pests, and erosion suspension agent.
At Lichuan, Hubei Province high mountain wild cabbage growing area, with gained biological and ecological methods to prevent plant disease, pests, and erosion suspension agent dilution 5 barrels of water of 200 times of every even spraying soil per mu (every barrel of 15 ~ 20kg water), mu suspension agent 1000mL.Transplant healthy seedling, transplant at wild cabbage 2 ~ 3 barrels of water of again spraying when punch the cave, every mu usage quantity be 500mL, to the field test preventive effect of club-root greater than 77.14%, strain weight 1.09kg.
Embodiment 7
getting 34kg Zulkovsky starch and 52kg mixed polysaccharide adds in 246kg water THE ADIABATIC SHEAR IN to mix well, add afterwards the standby scab streptomycete of 435kg (Streptomyces scabiei) WS-24926 zymocyte liquid, 48kg polyaryl phenol polyethenoxy ether, the 36kg alkylphenol polyoxyethylene, the 75kg sodium lignosulfonate, the 5kg polyglycol ether, the 69kg polyoxyethylene glycol, obtain mixture, dispersed with stirring is even, after grinding by the horizontal sand mill firsts and seconds, through granularity Detection, fineness of materials more than 90% reaches below 5 μ m, stop grinding and stirring, be packaged as finished product, namely obtain the biological and ecological methods to prevent plant disease, pests, and erosion suspension agent.
In experimental plot, Hubei Prov. Acdemy of Agricutural Sciences Wuhan, with knee by 1 ︰ 100(v/v) with the potting soil mixing after the dress alms bowl standby, with 90 times of the present embodiment streptomycete suspension agent dilutions, root 60 ~ 100mL is filled with in every strain when purple flowering stalk is transplanted, fill with again root after 10d 1 time, 60d " Invest, Then Investigate " preventive effect, streptomycete reaches 92% to the pot experiment prevention effect of purple flowering stalk club root, to plant without poisoning.
Embodiment 8
get the 25kg poly amic acid add with 144kg water in THE ADIABATIC SHEAR IN mix well, the zymocyte liquid that adds afterwards 680kg point spore streptomycete (Streptomyces cuspidosporus) WS-29246, the 20kg sodium lauryl sulphate, the 30kg alkylphenol polyoxyethylene, the 50kg potassium sorbate, the 15kg organosilicon, the 36kg triglycol, obtain mixture, dispersed with stirring is even, after grinding by the horizontal sand mill firsts and seconds, through granularity Detection, fineness of materials more than 90% reaches below 5 μ m, stop grinding and stirring, be packaged as finished product, namely obtain the biological and ecological methods to prevent plant disease, pests, and erosion suspension agent.
The high mountain wild cabbage growing area of bestowing favour in Hubei Province is with gained biological and ecological methods to prevent plant disease, pests, and erosion suspension agent dilution 5 barrels of water of 150 times of every even spraying soil per mu (every barrel of 15 ~ 20kg water), mu suspension agent 1000mL.Transplant healthy seedling, transplant at wild cabbage 2 ~ 3 barrels of water of again spraying when punch the cave, every mu usage quantity be 500mL, to the field test preventive effect of club-root greater than 75.22%, strain weight 1.13kg.
Embodiment 9
getting in the water that 23kg polyvinyl alcohol and 43kg sodium polyacrylate add 414kg THE ADIABATIC SHEAR IN mixes well, the zymocyte liquid mixed solution that adds afterwards 336kg scab streptomycete (Streptomyces scabiei) WS-24926 and sharp spore streptomycete (Streptomyces cuspidosporus) WS-29246, 11kg polyaryl phenol polyethenoxy ether, 66kg alkyl sulfo-succinic acid sodium salt, 22kg ethyl phenol Soxylat A 25-7, the 48kg ethyl p-hydroxybenzoate, the 12kg stearic acid, the 25kg propylene glycol, obtain mixture, dispersed with stirring is even, after grinding by the horizontal sand mill firsts and seconds, through granularity Detection, fineness of materials more than 90% reaches below 5 μ m, stop grinding and stirring, be packaged as finished product, namely obtain the biological and ecological methods to prevent plant disease, pests, and erosion suspension agent.
In experimental plot, Hubei Prov. Acdemy of Agricutural Sciences Wuhan, with knee by 1 ︰ 100(v/v) with the potting soil mixing after the dress alms bowl standby, the suspension agent of the present embodiment is diluted 150 times, root 60 ~ 100mL is filled with in every strain when purple flowering stalk is transplanted, fill with again root after 10d 1 time, 60d " Invest, Then Investigate " preventive effect, streptomycete reaches 89.3% to the pot experiment prevention effect of purple flowering stalk club root, to plant without poisoning.
Embodiment 10
Getting in the water that the 33kg polyvinyl alcohol adds 382kg THE ADIABATIC SHEAR IN mixes well, add afterwards the standby scab streptomycete of 442kg (Streptomyces scabiei) WS-24926 zymocyte liquid, 43kg sodium lignosulfonate, 15kg sorbic ester, 42kg Sodium Benzoate, 28kg isooctyl alcohol, 15kg glycol ether, obtain mixture, dispersed with stirring is even, after grinding by the horizontal sand mill firsts and seconds, through granularity Detection, fineness of materials more than 90% reaches below 5 μ m, stop grinding and stirring, be packaged as finished product, namely obtain the biological and ecological methods to prevent plant disease, pests, and erosion suspension agent.
In experimental plot, Hubei Prov. Acdemy of Agricutural Sciences Wuhan, with knee by 1 ︰ 100(v/v) with the potting soil mixing after the dress alms bowl standby, with 120 times of the present embodiment streptomycete suspension agent dilutions, root 90 ~ 120mL is filled with in every strain when purple flowering stalk is transplanted, fill with again root after 15d 1 time, 60d " Invest, Then Investigate " preventive effect, streptomycete reaches 81.08% to the pot experiment prevention effect of purple flowering stalk club root, to plant without poisoning.
Embodiment 11
Getting the 86kg xanthan gum adds in 449kg water THE ADIABATIC SHEAR IN to mix well, the zymocyte liquid, 95kg sodium lignosulfonate, 45kg alkyl alcohol Soxylat A 25-7,60kg potassium sorbate, 20kg defoamer lauric acid, the 15kg ethylene glycol that add afterwards 230kg point spore streptomycete (Streptomyces cuspidosporus) WS-29246, obtain mixture, dispersed with stirring is even, after grinding by the horizontal sand mill firsts and seconds, through granularity Detection, fineness of materials more than 90% reaches below 5 μ m, stop grinding and stirring, be packaged as finished product, namely obtain the biological and ecological methods to prevent plant disease, pests, and erosion suspension agent.
At Tujia Autonomous County of Changyang, Hubei Province high mountain wild cabbage growing area, with gained biological and ecological methods to prevent plant disease, pests, and erosion suspension agent dilution 5 barrels of water of 120 times of every even spraying soil per mu (every barrel of 15 ~ 20kg water), mu suspension agent 980mL.Transplant healthy seedling, transplant at wild cabbage 2 ~ 3 barrels of water of again spraying when punch the cave, every mu usage quantity be 600mL, to the preventive effect of club-root greater than 76.09%, strain weight 0.98kg.
Embodiment 12
getting the 20kg poly amic acid adds in 177kg water THE ADIABATIC SHEAR IN to mix well, the zymocyte liquid mixed solution that adds afterwards 723kg scab streptomycete (Streptomyces scabiei) WS-24926 and sharp spore streptomycete (Streptomyces cuspidosporus) WS-29246, the 12kg sodium lignosulfonate, the 15kg polyhydric alcohol fatty acid ester, the 35kg potassium sorbate, the 12kg polyglycol ether, the 6kg glycol ether, obtain mixture, dispersed with stirring is even, after grinding by the horizontal sand mill firsts and seconds, through granularity Detection, fineness of materials more than 90% reaches below 5 μ m, stop grinding and stirring, be packaged as finished product, namely obtain the biological and ecological methods to prevent plant disease, pests, and erosion suspension agent.
At Lichuan, Hubei Province high mountain wild cabbage growing area, with gained biological and ecological methods to prevent plant disease, pests, and erosion suspension agent dilution 6 barrels of water of 120 times of every even spraying soil per mu (every barrel of 15 ~ 20kg water), mu suspension agent 1100mL.Transplant healthy seedling, transplant at wild cabbage 2 ~ 3 barrels of water of again spraying when punch the cave, every mu usage quantity be 600mL, to the field test preventive effect of club-root greater than 78.02%, strain weight 1.03kg.
Embodiment 13
Getting the standby scab streptomycete of 1kg (Streptomyces scabiei) WS-24926 zymocyte liquid adopts sand mill grinding 1h to be added in reactor, get and be added in aforesaid reaction vessel after the 3kg gelatin is added in 100kg water dissolving, 45 ℃ of temperature controls, rotating speed is 400r/min rotation 60min, and adding weight percent is that 10% acetic acid transfers to 4～5 with system pH.Continue to stir 15min, adding 60 kg weight percents is 5% the gum arabic aqueous solution, adjusting rotary speed stirs 30min to 300r/min, adds 1.8kg formaldehyde solution, stirring reaction 10min, obtain the slow-release microcapsule aqueous solution, more centrifugal rear employing water cleans 1 ~ 2 time, more centrifugal concentrated solution, sieve after spraying drying after shaping, namely obtain slow releasing capsule.
Burn high mountain anti-season growth of Chinese cabbage district, level ground township (annual 5 ~ October) in the Tujia Autonomous County of Changyang, when Chinese cabbage is transplanted punching, sustained-release granular formulation 2g is spread fertilizer over the fields in every cave, 0.5 mu of ground of co-processing, at market vegetables ripening stage investigation club root incidence, calculate by statistics, it is 69.4% to Chinese cabbage club root field control effect.
Embodiment 14
Get the 3kg seaweed gel, add in the water of 97kg and dissolve.Zymocyte liquid, the employing sand mill of getting 2kg point spore streptomycete (Streptomyces cuspidosporus) WS-29246 grind 1h and are added in reactor.Get and be added in aforesaid reaction vessel after the 3kg gelatin is added in 97kg water dissolving, 45 ℃ of temperature controls, rotating speed are 300r/min rotation 60min, and adding weight percent is that 10% acetic acid transfers to 4 ~ 5 with system pH.Continue to stir 15min, add the seaweed gel aqueous solution of preparing, adjusting rotary speed stirs 30min to 300r/min.Add 1.8kg formaldehyde solution, stirring reaction 10min obtains the slow-release microcapsule aqueous solution, more centrifugal rear employing water cleans 3 times, more centrifugal concentrated solution, after the shaping of sieving after spraying drying, namely obtains slow releasing capsule.
In high mountain anti-season growth of Chinese cabbage district, He Jiaping town, Tujia Autonomous County of Changyang (annual 5 ~ October), when Chinese cabbage is transplanted punching, sustained-release granular formulation 2g is spread fertilizer over the fields in every cave, 0.5 mu of ground of co-processing, at market vegetables ripening stage investigation club root incidence, calculate by statistics, it is 74.5% to Chinese cabbage club root field control effect.
Embodiment 15
Get the 2kg seaweed gel, add in the water of 78kg and dissolve, get 3kg scab streptomycete (Streptomyces scabiei) WS-24926 and sharp spore streptomycete (Streptomyces cuspidosporus) WS-29246 the zymocyte liquid mixed solution, adopt sand mill to grind 1h to be added in reactor.Get and be added in aforesaid reaction vessel after the 3kg Xylo-Mucine is added in 96kg water dissolving, 45 ℃ of temperature controls, rotating speed are 400r/min rotation 10min, and adding weight percent is that 10% acetic acid transfers to 4 ~ 5 with system pH.Continue to stir 15min, add the seaweed gel aqueous solution of preparing, adjusting rotary speed stirs 30min to 300r/min.Add 1.8kg formaldehyde solution, stirring reaction 10min obtains the slow-release microcapsule aqueous solution, more centrifugal rear employing water cleans 1 ~ 2 time, more centrifugal concentrated solution, after the shaping of sieving after spraying drying slow releasing capsule.
High mountain anti-season growth of Chinese cabbage district (annual 5 ~ October) bestows favour in Hubei Province, when Chinese cabbage is transplanted punching, sustained-release granular formulation 2g is spread fertilizer over the fields in every cave, 0.5 mu of ground of co-processing, at market vegetables ripening stage investigation club root incidence, calculate by statistics, it is 71.6% to Chinese cabbage club root field control effect.
Embodiment 16
Get the gum arabic of 2kg, add in the water of 78kg and dissolve, get 2kg scab streptomycete (Streptomyces scabiei) WS-24926 and sharp spore streptomycete (Streptomyces cuspidosporus) WS-29246 the zymocyte liquid mixed solution, adopt sand mill to grind 1h to be added in reactor.Get and be added in aforesaid reaction vessel after the 5kg gelatin is added in 950kg water dissolving, 45 ℃ of temperature controls, rotating speed are 200r/min rotation 60min, and adding weight percent content is that 10% acetic acid transfers to 4 ~ 5 with system pH.Continue to stir 15min, add the gum arabic aqueous solution of preparing, adjusting rotary speed stirs 30min to 300r/min.Add 1.8kg formaldehyde solution, stirring reaction 10min obtains the slow-release microcapsule aqueous solution, more centrifugal rear employing water cleans 1 ~ 2 time, more centrifugal concentrated solution, after the shaping of sieving after spraying drying, namely obtains slow releasing capsule.
In anti-season growth of Chinese cabbage district, mountain, mayor Yanggao County, Lichuan (annual 5 ~ October), when Chinese cabbage is transplanted punching, sustained-release granular formulation 2g is spread fertilizer over the fields in every cave, 0.5 mu of ground of co-processing, at market vegetables ripening stage investigation club root incidence, calculate by statistics, it is 78.2% to Chinese cabbage club root field control effect.
Claims (7)
1. a streptomycete, comprise scab streptomycete (Streptomyces scabiei) WS-24926 CCTCC NO:M 2012393 and/or sharp spore streptomycete (Streptomyces cuspidosporus) WS-29246 CCTCC NO:M 2012413.
2. the application of the described streptomycete of claim 1 in control Cruciferae club root.
3. biological and ecological methods to prevent plant disease, pests, and erosion suspension agent that utilizes the control Cruciferae club root that the described streptomycete of claim 1 is prepared from, it is characterized in that: described biological and ecological methods to prevent plant disease, pests, and erosion suspension agent is comprised of 10 ~ 80% zymocyte liquid, 0.4 ~ 10% dispersion agent, 0.5 ~ 5% emulsifying agent, 0.4 ~ 10% thickening material, 0.4 ~ 10% sanitas, 0 ~ 3.0% defoamer, 0 ~ 10.0% frostproofer and the water of surplus by weight percentage, wherein, described zymocyte liquid is the bacterium liquid that is obtained by described streptomycete fermentation.
4. biological and ecological methods to prevent plant disease, pests, and erosion slow releasing capsule that utilizes the control cress club root that the described streptomycete of claim 1 is prepared from, it is characterized in that: described biological and ecological methods to prevent plant disease, pests, and erosion slow releasing capsule comprises gelatin or the Xylo-Mucine of the gum arabic of the zymocyte liquid of 1 ~ 3 part of weight, 1 ~ 3 part of weight or seaweed gel, 1 ~ 3 part of weight, wherein, described zymocyte liquid is the bacterium liquid that is obtained by described streptomycete fermentation.
5. biological and ecological methods to prevent plant disease, pests, and erosion suspension agent according to claim 3 is characterized in that: described dispersion agent be in sodium lignosulfonate, calcium lignin sulphonate, naphthalenesulfonic acid-formaldehyde condensate, fatty amide-N methyl taurine salt, alkyl sulfo-succinic acid sodium salt, sodium dibutyl naphthalene sulfonate, sodium lauryl sulphate, polyaryl phenol polyethenoxy ether and edetate choose any one kind of them or several; Described emulsifying agent is to choose any one kind of them in alkylbenzene sulfonate, alkyl alcohol Soxylat A 25-7, alkylphenol polyoxyethylene, styroyl phenol polyethenoxy ether, sorbic ester and polyhydric alcohol fatty acid ester or several; Described thickening material is that xanthan gum, gelatin, gum arabic, melon glue, sodium alginate, Zulkovsky starch, mixed polysaccharide, Xylo-Mucine, sodium hydroxyethlcellulose, polyvinyl alcohol, sodium polyacrylate, poly amic acid, urea, neusilin, silicon-dioxide, diatomite, wilkinite and Attapulgite are chosen any one kind of them or several; Described sanitas is that Sodium Benzoate, potassium sorbate and ethyl p-hydroxybenzoate are chosen any one kind of them or several; Described defoamer is to choose any one kind of them in isooctyl alcohol, primary isoamyl alcohol, stearic acid, lauric acid, polyglycol ether and organosilicon or several; Described frostproofer be in ethylene glycol, propylene glycol, glycerol, glycol ether, triglycol, polyoxyethylene glycol choose any one kind of them or several.
6. the preparation method of the described biological and ecological methods to prevent plant disease, pests, and erosion suspension agent of claim 3, is characterized in that, it comprises the following steps:
1) fermented liquid that described streptomycete fermentation is obtained, centrifugal 5 ~ 20min, obtain zymocyte liquid under 4000 ~ 10000r/min condition;
2) mix according to the water of described weight percent with described zymocyte liquid, dispersion agent, emulsifying agent, thickening material, sanitas, defoamer, frostproofer and surplus, obtain mixture;
3) mixture is sheared be uniformly dispersed after, be ground to 90% above raw meal particle size degree and reach below 5 μ m, stir, adjusting pH to 4.0 ~ 5.0 namely obtain the biological and ecological methods to prevent plant disease, pests, and erosion suspension agent.
7. the preparation method of the described biological and ecological methods to prevent plant disease, pests, and erosion slow releasing capsule of claim 4, is characterized in that, it comprises the following steps:
1) fermented liquid that first described streptomycete fermentation is obtained, centrifugal 5 ~ 20min, obtain zymocyte liquid under 4000 ~ 10000r/min condition, and the zymocyte liquid of getting 1 ~ 3 part of weight adds reaction vessel;
2) secondly, get the gelatin of 1 ~ 3 part of weight or Xylo-Mucine and add water to be configured to weight percent be after 3 ~ 8% the aqueous solution, to add reaction vessel, and with the pH value to 3 of acetum conditioned reaction thing ~ 5;
3) again, get the gum arabic of 1 ~ 3 part of weight or seaweed gel and add water to be mixed with weight percent be after 2 ~ 8% the aqueous solution, to add and so answer container, continue to stir 10 ~ 30min;
4) then, add formalin to solidify cyst wall in reaction vessel, continue to stir 10 ~ 30min, namely get the microcapsule aqueous solution;
5) last, will obtain spray drying treatment after microcapsule aqueous solution centrifugal treating, can obtain the biological and ecological methods to prevent plant disease, pests, and erosion slow releasing capsule.
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410128658.0A CN103911330B (en) | 2013-02-07 | 2013-02-07 | Point spore streptomycete and the application in preventing and treating Cruciferae clubroot thereof |
| CN201310049744.8A CN103173385B (en) | 2013-02-07 | 2013-02-07 | Streptomyces and applications thereof to prevention and control of clubroots of crucifers |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310049744.8A CN103173385B (en) | 2013-02-07 | 2013-02-07 | Streptomyces and applications thereof to prevention and control of clubroots of crucifers |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410128658.0A Division CN103911330B (en) | 2013-02-07 | 2013-02-07 | Point spore streptomycete and the application in preventing and treating Cruciferae clubroot thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103173385A true CN103173385A (en) | 2013-06-26 |
| CN103173385B CN103173385B (en) | 2014-11-05 |
Family
ID=48633579
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310049744.8A Active CN103173385B (en) | 2013-02-07 | 2013-02-07 | Streptomyces and applications thereof to prevention and control of clubroots of crucifers |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103173385B (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104663403A (en) * | 2015-03-06 | 2015-06-03 | 四川省农业科学院植物保护研究所 | Floating seedling raising method capable of preventing and controlling club roots of cruciferae crops |
| CN104004526B (en) * | 2014-06-16 | 2017-02-22 | 四川省农业科学院植物保护研究所 | Soil conditioner for preventing and treating clubroot diseases of cruciferae crops, and preparing method and application of soil conditioner |
| CN114176097A (en) * | 2021-11-15 | 2022-03-15 | 贵州省植物保护研究所 | Pesticide composition of seed treatment suspending agent for preventing and treating clubroot of cruciferous vegetables |
| CN117187121A (en) * | 2023-08-28 | 2023-12-08 | 广西地源之本肥业有限公司 | Kangshi grass-inhibiting bacteria and application thereof |
-
2013
- 2013-02-07 CN CN201310049744.8A patent/CN103173385B/en active Active
Non-Patent Citations (3)
| Title |
|---|
| G.PENG等: "Potential biological control of clubroot on canola and crucifer vegetable croops", 《PLANT PATHOLOGY》 * |
| 王靖等: "两株根肿病生防放线菌的鉴定及其防病效果", 《中国农业科学》 * |
| 王靖等: "油菜根肿病菌拮抗微生物的筛选及其防治效果测定", 《中国油料作物学报》 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104004526B (en) * | 2014-06-16 | 2017-02-22 | 四川省农业科学院植物保护研究所 | Soil conditioner for preventing and treating clubroot diseases of cruciferae crops, and preparing method and application of soil conditioner |
| CN104663403A (en) * | 2015-03-06 | 2015-06-03 | 四川省农业科学院植物保护研究所 | Floating seedling raising method capable of preventing and controlling club roots of cruciferae crops |
| CN114176097A (en) * | 2021-11-15 | 2022-03-15 | 贵州省植物保护研究所 | Pesticide composition of seed treatment suspending agent for preventing and treating clubroot of cruciferous vegetables |
| CN117187121A (en) * | 2023-08-28 | 2023-12-08 | 广西地源之本肥业有限公司 | Kangshi grass-inhibiting bacteria and application thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN103173385B (en) | 2014-11-05 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103342604B (en) | Bio-control bacterium foliage fertilizer, and preparation method and application thereof | |
| CN100334201C (en) | Bacillus subtilis and its uses | |
| CN102876576B (en) | Acidic soil conditioner, and preparation method and application thereof | |
| CN102925386B (en) | Bacillus amyloliquefaciens and application thereof in prevention and treatment of walnut anthracnose | |
| CN106164247A (en) | Inoculation microbial inoculum for stress soil | |
| AU2016389840A1 (en) | Microbial consortia | |
| CN103074271B (en) | Bacillus subtilis YB-05, microbial preparation thereof, and application of Bacillus subtilis YB-05 or microbial preparation | |
| Hahn et al. | Rhizobial inoculation, alone or coinoculated with Azospirillum brasilense, promotes growth of wetland rice | |
| CN101481668A (en) | Bacillus thuringiensis suspending agent for killing cotton spider mites, preparation and use | |
| CN110205273A (en) | A kind of bacillus amyloliquefaciens and its application with growth promotion and resistant effect | |
| CN109852565A (en) | A kind of salt-soda soil composite modifying agent and its method of administration | |
| CN109679884A (en) | One plant of efficient Promoting bacteria of corn that can be reduced nitrogen phosphorus fertilizer application and its application | |
| CN103911330B (en) | Point spore streptomycete and the application in preventing and treating Cruciferae clubroot thereof | |
| CN103173385B (en) | Streptomyces and applications thereof to prevention and control of clubroots of crucifers | |
| CN105154339B (en) | A kind of Trichoderma viride bacteria strain and its application | |
| CN105132296B (en) | A kind of hook-shaped trichoderma strain and its application | |
| CN101892173A (en) | Bacillus subtilis with bio-control effect on continuous cropping diseases of strawberries and application thereof | |
| CN106967635A (en) | A kind of preparation method for the biocontrol agent for preventing and treating apple tree fungal disease | |
| CN106146194A (en) | A kind of anti-continuous cropping microbial inoculum | |
| CN104830706B (en) | One plant of bacillusmusilaginosiengineering and the organic sustained-controll-release fertiliser of potato special bio | |
| CN107857681A (en) | A kind of special fertilizer for cole and preparation method and application | |
| CN102533564B (en) | A kind of screening method of bio-control trichoderma in corn seedling stage root rot period | |
| CN104403976A (en) | Antagonistic actinomycete for preventing and controlling continuously cropped strawberry root rot and preparation method of biocontrol agent of antagonistic actinomycete | |
| CN108477221B (en) | Method for preventing and treating root-knot nematode by using biological pesticide | |
| CN101619293B (en) | Streptomyces vinaceusdrappus, filtering method and application |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant |