CN103169738A - Method for removing heavy metal from ganoderma extract - Google Patents
Method for removing heavy metal from ganoderma extract Download PDFInfo
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- CN103169738A CN103169738A CN2013101037225A CN201310103722A CN103169738A CN 103169738 A CN103169738 A CN 103169738A CN 2013101037225 A CN2013101037225 A CN 2013101037225A CN 201310103722 A CN201310103722 A CN 201310103722A CN 103169738 A CN103169738 A CN 103169738A
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Abstract
The invention discloses a method for removing heavy metal from ganoderma extract. The method sequentially comprises the following steps of weighing lucid ganoderma, coarsely crushing; adding water and boiling for extracting to obtain an extracting solution; filtering the extracting solution, concentrating the filtrate until the volume of the solution is 2-4fold(v/m) of lucid ganoderma, standing and cooling for 1-2 hours; centrifuging, and carrying out alumina column chromatography on centrifugate, wherein the sampling ratio of aluminum oxide to lucid ganoderma is (1:2) to (1:7), the ratio of the column diameter to the column height is (1:5) to (1:10), and the flow speed is 1.2-2.0ml/min; collecting the liquid generated after alumina column chromatography, concentrating the liquid until the specific gravity is 1.2; drying in a vacuum drying oven for 2.5-3 hours at 70 DEG C, and crushing to obtain the product. The method disclosed by the invention is used for removing the heavy metal from the ganoderma extract, and especially has good removal effect on heavy metal such as arsenic, lead and cadmium.
Description
Technical field
The present invention relates to the technical field that removes of residual chemical composition in plant extract, particularly, relate to a kind of method that removes heavy metal in Ganoderma extract.
Background technology
Ganoderma is basidiomycetes Polyporaceae Ganoderma plant, and its growth course comprises sporophore, mycelium and spore powder three phases.Ganoderma just is considered to a kind of effective strengthening by means of tonics medicine since ancient times, what its application was maximum is the sporophore of Ganoderma, studies show that, the main chemical compositions of Ganoderma has polysaccharide, ucleosides, three-iron acids, sterol, alkaloids and aminoacid etc., and its hypoxia-bearing capability and all many-side such as resisting fatigue and antitumor at calmness, heart tonifying, defying age, raising body has effect widely.Thereby be used to prepare various medicines, food, beverage and cosmetic health product.Ganoderma extract is take Ganoderma sporophore and Ganoderma spore powder as raw material, concentrate drying after solvent extraction and making, and its functional effect is equal to the Ganoderma medical material.
Extremely pay close attention in the world the content problem of heavy metal in Chinese medicine, it has become affects the Chinese medicine outlet, enter one of main restricting factor of international market.Therefore, reduce the content of heavy metal in the Ganoderma medicinal substances extract, the requirement that initiatively adapts to globalization is very urgent key subjects.
Summary of the invention
Technical problem to be solved by this invention is to provide a kind of method that removes heavy metal in Ganoderma extract, and the method is used for removing the Ganoderma extract heavy metal, and it is good especially to heavy metal arsenic, lead, cadmium removal effect.
The present invention solves the problems of the technologies described above the technical scheme that adopts: a kind of method that removes heavy metal in Ganoderma extract in turn includes the following steps:
Step (1) takes the Ganoderma medical material, coarse pulverization;
Step (2) adds water boil and extracts, and obtains extracting solution;
Step (3), with the extracting liquid filtering that described step (2) obtains, it is that the 2-4 of Ganoderma medical material doubly measures (v/m) that filtrate is concentrated into liquor capacity, standingly lets cool 1-2 hour;
Step (4), centrifugal, alumina column chromatography on centrifugal liquid, loading ratio aluminium oxide: medical material=1:2～1:7, the post footpath: post is high=1:5～1:10, flow velocity 1.2～2.0ml/min collects lower column liquid, is concentrated into proportion 1.2;
Step (5) was put in 70 ℃ of vacuum drying ovens dry 2.5～3 hours, pulverized, and got product.
Described step (2) concrete operations are: in the Ganoderma medical material that described step (1) is pulverized, gradation adds the water boil of 10 times of amounts of described Ganoderma medical material and 8 times of amounts to extract 2 times, and boiling time is respectively 2h, 1.5h.
In described step (3), filtrate is concentrated into 3 times of amounts (v/m) that liquor capacity is described step (1) Ganoderma medical material.
In described step (4), described proportion adopts Baume instrumentation amount.
The present invention solves the problems of the technologies described above the technical scheme that adopts and also comprises the Ganoderma extract that adopts said method to prepare is provided.
Further, in described Ganoderma extract, arsenic content is 0.11-0.24ppm, and lead content is 0.02-0.17ppm, and cadmium content is 0.14-0.28ppm.
The present invention compared with prior art has following advantage: the inventive method is used for removing the Ganoderma extract heavy metal, and it is good especially to heavy metal arsenic, lead, cadmium removal effect.
The specific embodiment
In order to understand better content of the present invention, be described further below in conjunction with specific embodiment.Should be understood that these embodiment only for the present invention is further described, limit the scope of the invention and be not used in.Should be understood that in addition after having read content of the present invention, the person skilled in art makes some nonessential change or adjustment to the present invention, still belongs to protection scope of the present invention.
Embodiment 1
get 100g Ganoderma medical material, coarse pulverization, gradation adds 10 times of amounts of medical material (v/m) and 8 times of amounts (v/m) water boil to extract 2 times, boiling time is respectively 2h, 1.5h, extracting liquid filtering, filtrate is concentrated into 2 times of amounts (v/m) that liquor capacity is the Ganoderma medical material, standingly let cool 1-2 hour, centrifugal, alumina column chromatography on centrifugal liquid, loading ratio aluminium oxide: medical material=1:2～1:7, the post footpath: post is high=1:5～1:10, flow velocity 1.2ml/min, collect lower column liquid, be concentrated into proportion 1.20(Baume instrumentation amount), put in 70 ℃ of vacuum drying ovens dry 2.7 hours, pulverize, cross 80 mesh sieves, get product.
Embodiment 2
get 100g Ganoderma medical material, coarse pulverization, add 10 times of amounts of medical material (v/m) and 8 times of amounts (v/m) water boil to extract 2 times, boiling time is respectively 2h, 1.5h, extracting liquid filtering, filtrate is concentrated into 3 times of amounts (v/m) that liquor capacity is the Ganoderma medical material, standingly let cool 1-2 hour, centrifugal, alumina column chromatography on centrifugal liquid, loading ratio aluminium oxide: medical material=1:2～1:7, the post footpath: post is high=1:5～1:10, flow velocity 2.0ml/min, collect lower column liquid, be concentrated into proportion 1.20(Baume instrumentation amount), put in 70 ℃ of vacuum drying ovens dry 2.5 hours, pulverize, cross 80 mesh sieves, get product.
Embodiment 3
get 100g Ganoderma medical material, coarse pulverization, add 10 times of amounts of medical material (v/m) and 8 times of amounts (v/m) water boil to extract 2 times, boiling time is respectively 2h, 1.5h, extracting liquid filtering, filtrate is concentrated into 4 times of amounts (v/m) that liquor capacity is the Ganoderma medical material, standingly let cool 1-2 hour, centrifugal, alumina column chromatography on centrifugal liquid, loading ratio aluminium oxide: medical material=1:2～1:7, the post footpath: post is high=1:5～1:10, flow velocity 1.6ml/min, collect lower column liquid, be concentrated into proportion 1.20(Baume instrumentation amount), put in 70 ℃ of vacuum drying ovens dry 3 hours, pulverize, cross 80 mesh sieves, get product.
Measure the content of beary metal that removes front and back of embodiment 1-3.
As above, just can realize preferably the present invention.
Claims (6)
1. a method that removes heavy metal in Ganoderma extract, is characterized in that, in turn includes the following steps:
Step (1) takes the Ganoderma medical material, coarse pulverization;
Step (2) adds water boil and extracts, and obtains extracting solution;
Step (3), with the extracting liquid filtering that described step (2) obtains, it is that the 2-4 of Ganoderma medical material doubly measures (v/m) that filtrate is concentrated into liquor capacity, standingly lets cool 1-2 hour;
Step (4), centrifugal, alumina column chromatography on centrifugal liquid, loading ratio aluminium oxide: medical material=1:2～1:7, the post footpath: post is high=1:5～1:10, flow velocity 1.2～2.0ml/min collects lower column liquid, is concentrated into proportion 1.2;
Step (5) was put in 70 ℃ of vacuum drying ovens dry 2.5～3 hours, pulverized, and got product.
2. a kind of method that removes heavy metal in Ganoderma extract according to claim 1, it is characterized in that, described step (2) concrete operations are: in the Ganoderma medical material that described step (1) is pulverized, gradation adds the water boil of 10 times of amounts of described Ganoderma medical material and 8 times of amounts to extract 2 times, and boiling time is respectively 2h, 1.5h.
3. a kind of method that removes heavy metal in Ganoderma extract according to claim 1, is characterized in that, in described step (3), filtrate is concentrated into 3 times of amounts (v/m) that liquor capacity is described step (1) Ganoderma medical material.
4. a kind of method that removes heavy metal in Ganoderma extract according to claim 1, is characterized in that, in described step (4), described proportion adopts Baume instrumentation amount.
5. the Ganoderma extract that adopts said method to prepare.
6. Ganoderma extract according to claim 5, is characterized in that, arsenic content is 0.11-0.24ppm, and lead content is 0.02-0.17ppm, and cadmium content is 0.14-0.28ppm.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN201310103722.5A CN103169738B (en) | 2013-03-28 | 2013-03-28 | Method for removing heavy metal from ganoderma extract |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201310103722.5A CN103169738B (en) | 2013-03-28 | 2013-03-28 | Method for removing heavy metal from ganoderma extract |
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| CN103169738A true CN103169738A (en) | 2013-06-26 |
| CN103169738B CN103169738B (en) | 2015-01-28 |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111213817A (en) * | 2020-03-25 | 2020-06-02 | 陕西嘉禾药业有限公司 | Method for removing heavy metal arsenic in oyster mushroom extract |
| CN112772825A (en) * | 2021-01-04 | 2021-05-11 | 山西昇力元生物科技有限公司 | Method for reducing heavy metal cadmium in ganoderma lucidum spore powder |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1363391A (en) * | 2001-01-04 | 2002-08-14 | 李晔 | Garnoderma product and its preparing process |
| CN1704156A (en) * | 2004-06-04 | 2005-12-07 | 天津大学 | Adsorbent for deeply purifying trace quantity arsenic in plant extract and preparation method thereof |
| CN101948150A (en) * | 2010-09-10 | 2011-01-19 | 中国科学院化学研究所 | Method for purifying water |
-
2013
- 2013-03-28 CN CN201310103722.5A patent/CN103169738B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1363391A (en) * | 2001-01-04 | 2002-08-14 | 李晔 | Garnoderma product and its preparing process |
| CN1704156A (en) * | 2004-06-04 | 2005-12-07 | 天津大学 | Adsorbent for deeply purifying trace quantity arsenic in plant extract and preparation method thereof |
| CN101948150A (en) * | 2010-09-10 | 2011-01-19 | 中国科学院化学研究所 | Method for purifying water |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111213817A (en) * | 2020-03-25 | 2020-06-02 | 陕西嘉禾药业有限公司 | Method for removing heavy metal arsenic in oyster mushroom extract |
| CN112772825A (en) * | 2021-01-04 | 2021-05-11 | 山西昇力元生物科技有限公司 | Method for reducing heavy metal cadmium in ganoderma lucidum spore powder |
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| CN103169738B (en) | 2015-01-28 |
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