CN103169738B - Method for removing heavy metal from ganoderma extract - Google Patents
Method for removing heavy metal from ganoderma extract Download PDFInfo
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- CN103169738B CN103169738B CN201310103722.5A CN201310103722A CN103169738B CN 103169738 B CN103169738 B CN 103169738B CN 201310103722 A CN201310103722 A CN 201310103722A CN 103169738 B CN103169738 B CN 103169738B
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Abstract
The invention discloses a method for removing heavy metal from ganoderma extract. The method sequentially comprises the following steps of weighing lucid ganoderma, coarsely crushing; adding water and boiling for extracting to obtain an extracting solution; filtering the extracting solution, concentrating the filtrate until the volume of the solution is 2-4fold(v/m) of lucid ganoderma, standing and cooling for 1-2 hours; centrifuging, and carrying out alumina column chromatography on centrifugate, wherein the sampling ratio of aluminum oxide to lucid ganoderma is (1:2) to (1:7), the ratio of the column diameter to the column height is (1:5) to (1:10), and the flow speed is 1.2-2.0ml/min; collecting the liquid generated after alumina column chromatography, concentrating the liquid until the specific gravity is 1.2; drying in a vacuum drying oven for 2.5-3 hours at 70 DEG C, and crushing to obtain the product. The method disclosed by the invention is used for removing the heavy metal from the ganoderma extract, and especially has good removal effect on heavy metal such as arsenic, lead and cadmium.
Description
Technical field
The present invention relates to the removing sulfuldioxide field of chemical residue composition in plant extract, particularly, relate to a kind of method removing heavy metal in Ganoderma extract.
Background technology
Ganoderma is basidiomycetes Polyporaceae Ganoderma plant, and its growth course comprises sporophore, mycelium and spore powder three phases.Ganoderma is just considered to a kind of effective strengthening by means of tonics medicine since ancient times, its application is maximum is the sporophore of Ganoderma, research shows, the main chemical compositions of Ganoderma has polysaccharide, ucleosides, three-iron acids, sterol, alkaloids and aminoacid etc., and its hypoxia-bearing capability at calmness, heart tonifying, defying age, raising body and all many-sides such as resisting fatigue and antitumor have and act on widely.Thus be used to prepare various medicine, food, beverage and cosmetic health product.Ganoderma extract is with Ganoderma sporophore and Ganoderma spore powder for raw material, and concentrate drying after solvent extraction and obtaining, its functional effect is equal to Ganoderma medical material.
Very pay close attention to the content problem of heavy metal in Chinese medicine in the world, it has become affects one of Chinese medicine outlet, the main restricting factor entering international market.Therefore, reduce the content of heavy metal in Ganoderma medicinal substances extract, the requirement of Initial adaption globalization is very urgent key subjects.
Summary of the invention
Technical problem to be solved by this invention is to provide a kind of method removing heavy metal in Ganoderma extract, and the method is used for removing heavy metal in Ganoderma extract, and its heavy metal arsenic, lead, cadmium removal effect are good especially.
The present invention solves the problems of the technologies described above adopted technical scheme: a kind of method removing heavy metal in Ganoderma extract, in turn includes the following steps:
Step (1), takes Ganoderma medical material, coarse pulverization;
Step (2), adds water boil and extracts, obtain extracting solution;
Step (3), by the extracting liquid filtering that described step (2) obtains, filtrate is concentrated into the 2-4 times amount (v/m) that liquor capacity is Ganoderma medical material, leaves standstill and lets cool 1-2 hour;
Step (4), centrifugal, alumina column chromatography in centrifugal liquid, loading ratio aluminium oxide: medical material=1:2 ~ 1:7, post footpath: post is high=1:5 ~ 1:10, flow velocity 1.2 ~ 2.0ml/min, collect lower column liquid, be concentrated into proportion 1.2;
Step (5), to put in 70 DEG C of vacuum drying ovens dry 2.5 ~ 3 hours, pulverizes, gets product.
Described step (2) concrete operations are: in the Ganoderma medical material that described step (1) is pulverized, the water boil that gradation adds described Ganoderma medical material 10 times amount and 8 times amount extracts 2 times, and boiling time is respectively 2h, 1.5h.
In described step (3), filtrate is concentrated into 3 times amount (v/m) that liquor capacity is described step (1) Ganoderma medical material.
In described step (4), described proportion adopts Baume measurement amount.
The present invention solves the problems of the technologies described above adopted technical scheme and also comprises the Ganoderma extract providing and adopt said method to prepare.
Further, in described Ganoderma extract, arsenic content is 0.11-0.24ppm, and lead content is 0.02-0.17ppm, and cadmium content is 0.14-0.28ppm.
Compared with prior art, tool has the following advantages in the present invention: the inventive method is used for for removing heavy metal in Ganoderma extract, and its heavy metal arsenic, lead, cadmium removal effect are good especially.
Detailed description of the invention
In order to understand content of the present invention better, be described further below in conjunction with specific embodiment.Should be understood that these embodiments only for the present invention is further described, and be not used in and limit the scope of the invention.In addition should be understood that, after having read content of the present invention, person skilled in art makes some nonessential change or adjustment to the present invention, still belongs to protection scope of the present invention.
Embodiment 1
Get 100g Ganoderma medical material, coarse pulverization, gradation adds medical material 10 times amount (v/m) and 8 times amount (v/m) water boil extracts 2 times, boiling time is respectively 2h, 1.5h, extracting liquid filtering, filtrate is concentrated into 2 times amount (v/m) that liquor capacity is Ganoderma medical material, leave standstill and let cool 1-2 hour, centrifugal, alumina column chromatography in centrifugal liquid, loading ratio aluminium oxide: medical material=1:2 ~ 1:7, post footpath: post is high=1:5 ~ 1:10, flow velocity 1.2ml/min, collect lower column liquid, be concentrated into proportion 1.20(Baume measurement amount), to put in 70 DEG C of vacuum drying ovens dry 2.7 hours, pulverize, cross 80 mesh sieves, get product.
Embodiment 2
Get 100g Ganoderma medical material, coarse pulverization, add medical material 10 times amount (v/m) and 8 times amount (v/m) water boil extracts 2 times, boiling time is respectively 2h, 1.5h, extracting liquid filtering, filtrate is concentrated into 3 times amount (v/m) that liquor capacity is Ganoderma medical material, leave standstill and let cool 1-2 hour, centrifugal, alumina column chromatography in centrifugal liquid, loading ratio aluminium oxide: medical material=1:2 ~ 1:7, post footpath: post is high=1:5 ~ 1:10, flow velocity 2.0ml/min, collect lower column liquid, be concentrated into proportion 1.20(Baume measurement amount), to put in 70 DEG C of vacuum drying ovens dry 2.5 hours, pulverize, cross 80 mesh sieves, get product.
Embodiment 3
Get 100g Ganoderma medical material, coarse pulverization, add medical material 10 times amount (v/m) and 8 times amount (v/m) water boil extracts 2 times, boiling time is respectively 2h, 1.5h, extracting liquid filtering, filtrate is concentrated into 4 times amount (v/m) that liquor capacity is Ganoderma medical material, leave standstill and let cool 1-2 hour, centrifugal, alumina column chromatography in centrifugal liquid, loading ratio aluminium oxide: medical material=1:2 ~ 1:7, post footpath: post is high=1:5 ~ 1:10, flow velocity 1.6ml/min, collect lower column liquid, be concentrated into proportion 1.20(Baume measurement amount), to put in 70 DEG C of vacuum drying ovens dry 3 hours, pulverize, cross 80 mesh sieves, get product.
Measure the content of beary metal removing front and back of embodiment 1-3.
As above, just the present invention can be realized preferably.
Claims (5)
1. remove a method for heavy metal in Ganoderma extract, it is characterized in that, in turn include the following steps:
Step (1), takes Ganoderma medical material, coarse pulverization;
Step (2), adds water boil and extracts, obtain extracting solution;
Step (3), by the extracting liquid filtering that described step (2) obtains, filtrate is concentrated into the 2-4 times amount (v/m) that liquor capacity is Ganoderma medical material, leaves standstill and lets cool 1-2 hour;
Step (4), centrifugal, alumina column chromatography in centrifugal liquid, loading ratio aluminium oxide: medical material=1:2 ~ 1:7, post footpath: post is high=1:5 ~ 1:10, flow velocity 1.2 ~ 2.0ml/min, collect lower column liquid, be concentrated into proportion 1.2;
Step (5), to put in 70 DEG C of vacuum drying ovens dry 2.5 ~ 3 hours, pulverizes, gets product;
Described step (2) concrete operations are: in the Ganoderma medical material that described step (1) is pulverized, the water boil that gradation adds described Ganoderma medical material 10 times amount and 8 times amount extracts 2 times, and boiling time is respectively 2h, 1.5h.
2. a kind of method removing heavy metal in Ganoderma extract according to claim 1, is characterized in that, in described step (3), filtrate is concentrated into 3 times amount (v/m) that liquor capacity is described step (1) Ganoderma medical material.
3. a kind of method removing heavy metal in Ganoderma extract according to claim 1, is characterized in that, in described step (4), described proportion adopts Baume measurement amount.
4. adopt the Ganoderma extract that described in claim 1-3 any one, method prepares.
5. Ganoderma extract according to claim 4, is characterized in that, arsenic content is 0.11-0.24ppm, and lead content is 0.02-0.17ppm, and cadmium content is 0.14-0.28ppm.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN201310103722.5A CN103169738B (en) | 2013-03-28 | 2013-03-28 | Method for removing heavy metal from ganoderma extract |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201310103722.5A CN103169738B (en) | 2013-03-28 | 2013-03-28 | Method for removing heavy metal from ganoderma extract |
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| CN103169738A CN103169738A (en) | 2013-06-26 |
| CN103169738B true CN103169738B (en) | 2015-01-28 |
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Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN111213817A (en) * | 2020-03-25 | 2020-06-02 | 陕西嘉禾药业有限公司 | Method for removing heavy metal arsenic in oyster mushroom extract |
| CN112772825A (en) * | 2021-01-04 | 2021-05-11 | 山西昇力元生物科技有限公司 | Method for reducing heavy metal cadmium in ganoderma lucidum spore powder |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1363391A (en) * | 2001-01-04 | 2002-08-14 | 李晔 | Garnoderma product and its preparing process |
| CN1704156A (en) * | 2004-06-04 | 2005-12-07 | 天津大学 | Adsorbent for deeply purifying trace quantity arsenic in plant extract and preparation method thereof |
| CN101948150A (en) * | 2010-09-10 | 2011-01-19 | 中国科学院化学研究所 | Method for purifying water |
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- 2013-03-28 CN CN201310103722.5A patent/CN103169738B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1363391A (en) * | 2001-01-04 | 2002-08-14 | 李晔 | Garnoderma product and its preparing process |
| CN1704156A (en) * | 2004-06-04 | 2005-12-07 | 天津大学 | Adsorbent for deeply purifying trace quantity arsenic in plant extract and preparation method thereof |
| CN101948150A (en) * | 2010-09-10 | 2011-01-19 | 中国科学院化学研究所 | Method for purifying water |
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| CN103169738A (en) | 2013-06-26 |
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