CN103127266A - Loquat-shaped cake diuretic and extraction and preparation method thereof - Google Patents
Loquat-shaped cake diuretic and extraction and preparation method thereof Download PDFInfo
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- CN103127266A CN103127266A CN2011103854217A CN201110385421A CN103127266A CN 103127266 A CN103127266 A CN 103127266A CN 2011103854217 A CN2011103854217 A CN 2011103854217A CN 201110385421 A CN201110385421 A CN 201110385421A CN 103127266 A CN103127266 A CN 103127266A
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Abstract
The invention relates to a medicine made of loquat-shaped cakes and with a diuretic function, in particular to a loquat-shaped cake diuretic and an extraction and preparation method thereof. The loquat-shaped cake diuretic is characterized by being directly prepared and obtained from the loquat-shaped cakes, belonging to purely natural content, and being obvious in a diuretic effect. The loquat-shaped cake diuretic is taken orally, and is convenient to carry, easy to store, easy, convenient and feasible in extraction and preparation process, and capable of being produced in a large scale.
Description
Technical field
The present invention relates to a kind of medicine with diuresis of being made by Fractus Eriobotryae, particularly relate to a kind of Fractus Eriobotryae diuretic and extract preparation method.
Technical background
Folium Eriobotryae is very extensive in China's plantation, and Fractus Eriobotryae is rich in the various nutrients of needed by human body, is the health fruit of nutrition, is also the fruit with high medical value.Fractus Eriobotryae rich cellulose, pectin, carotene, malic acid, citric acid, potassium, phosphorus, ferrum, calcium and vitamin A, B, C.Traditional Chinese medical science record, Radix Eriobotryae is bitter, flat, nontoxic, and the effect of preventing phlegm from forming and stopping coughing, soothing the liver circulation of qi promoting, inducing diuresis to remove edema is arranged.Be used for cough with copious phlegm, hernia, edema, scrofula.Loquat is sweet, sour, cool, nontoxic, and the effect of nourishing the lung to keep the adverse QI downward is arranged, quenching the thirst is used for the lung-heat cough with asthma, tells contrary, excessive thirst.Cough-relieving, the antitussive medicine made take Folium Eriobotryae as raw material at present are more, but the diuresis of Folium Eriobotryae not yet draws attention, and the diuresis of loquat fruit is without record, and the extraction of Folium Eriobotryae diuresis material preparation there is not yet report.
The invention reside in from loquat to extract and have the natural component of diuretic activity, then it is prepared into the oral drugs that are easy to carry, preserve, simple process can carry out large-scale production, thereby improves the comprehensive utilization value of loquat.
Summary of the invention
The purpose of this invention is to provide a kind of Fractus Eriobotryae diuretic and extract preparation method; be characterized in extracting from Folium Eriobotryae and prepare the natural component with diuretic activity; it is prepared into the oral diuretic that is easy to carry, preserves, extraction and preparation technique is simple and feasible, is produced on a large scale.The present invention relates to a kind of Fractus Eriobotryae diuretic and extract preparation method, medicine of the present invention can extract preparation as follows:
1. the extraction of Fractus Eriobotryae diuretic:
The first extracting method of Fractus Eriobotryae diuretic: with Fractus Eriobotryae cleaning, pulverizing, drying, first water decocts to get decocting liquid; Then residue is extracted with the organic solvent of different volumes ratio and the mixed solvent of water successively, the volume ratio of organic solvent and water is 40: 60,60: 40,80: 20,95: 5, abandons at last residue; Merge above decocting liquid and mixed solvent extracting solution, get the full extract of Fractus Eriobotryae.
The second extracting method of Fractus Eriobotryae diuretic: with Fractus Eriobotryae cleaning, pulverizing, drying, extract with the organic solvent of different volumes ratio and the mixed solvent of water successively, the volume ratio of organic solvent and water is 95: 5,80: 20,60: 40,40: 60; Residue after extraction is used water extraction again, last waste; Merge above mixed solvent extracting solution and aqueous extract, get the full extract of Fractus Eriobotryae.
The organic solvent that relates in the extraction of above Fractus Eriobotryae diuretic is a kind of in methanol, ethanol or acetone, and extracting mode is that a kind of or combination of merceration extraction, heating extraction, diafiltration extraction, reflux, extract,, supersound extraction, microwave extraction mode is used.
2. Fractus Eriobotryae diuresis preparation approximately:
According to the full extract that said method makes, further refining, obtain Fractus Eriobotryae diuretic oral drugs.
The first preparation method of Fractus Eriobotryae diuretic: the full extract that will extract gained is condensed into the dope of distinguishing the flavor of without alcohol, be adjusted to again 1/5th to 1/8th volumes of former full extract volume with suitable quantity of water, then use defat with petroleum ether, concentrate drying after defat obtains Fractus Eriobotryae diuresis crude extract.Crude extract is further refining with macroporous resin, first uses the pure water eluting, removes strong polar fraction; Then use successively 20%-95% alcoholic solution eluting, merge pure water mixed solvent eluting section, make oral drugs after concentrate drying.
The second preparation method of Fractus Eriobotryae diuretic: the full extract that will extract gained is condensed into the dope of distinguishing the flavor of without alcohol, then is adjusted to 1/5th to 1/8th volumes of former full extract volume with suitable quantity of water; Then use successively petroleum ether, ethyl acetate, n-butanol extraction; Combined ethyl acetate extraction section and n-butanol extraction section are made oral drugs after concentrate drying.
The oral drugs that are prepared into are granule, tablet, capsule.
3. the diuretic activity of Fractus Eriobotryae diuretic experiment (testing the extraction preparation method of Fractus Eriobotryae diuretic used with reference to embodiment two)
1. test method: mouse metabolism cage method
The animal grouping: get 24 of mices, be divided at random 4 groups, 6 every group, male and female half and half.
Experimental technique: before experiment, water 12h is can't help in fasting, and during experiment, water is prohibited in fasting.The light mice lower abdomen of pressing drains remaining urine, and administration time is recorded in each treated animal per os gavage (ig) administration.Mice is put into respectively homemade metabolic cage, per hour use the electronic balance weighing urine collector 1 time, Continuous Observation record 7 hours.
Dosage: diuretic dosage of the present invention is 34mg/kg; The blank group gives the distilled water of equal volume; If two positive groups, the hydrochlorothiazide dosage is 25mg/kg, and quick-acting diuretic FRUSEMIDE dosages are 40mg/kg.Every day 1 time, measured 1 hour and 7 hours urine amounts.Above experiment repeated measure 6 times.
2. experimental result
The different samples of table 1 on the impact of mouse retention amount (
N=10)
Annotate: there were significant differences to organize relatively urine amount increase with blank,
*P＜0.05,
*P＜0.01,
* *P＜0.001
The specific embodiment
Embodiment one
With Fractus Eriobotryae cleaning, pulverizing, drying, carry out the diafiltration extraction with the ethanol of different volumes ratio and the mixed solvent of water successively, the volume ratio of ethanol and water is 95: 5,80: 20,60: 40,40: 60; Residue after extraction water diafiltration again extracts, last waste; Merge above mixed solvent extracting solution and aqueous extract, get the full extract of Fractus Eriobotryae.
The full extract of gained is condensed into the dope of distinguishing the flavor of without alcohol, then is adjusted to 1/5th to 1/8th volumes of former full extract volume with suitable quantity of water, then use defat with petroleum ether, concentrate drying after defat obtains Fractus Eriobotryae diuresis crude extract.Crude extract is further refining with macroporous resin, first uses the pure water eluting, removes strong polar fraction; Then use successively the alcohol-water (V/V) of 20: 80,40: 60,60: 40,80: 20,95: 5 to carry out gradient elution, merge pure water mixed solvent eluting section, concentrate drying is also pulverized, and crosses 80 mesh sieves, in incapsulating, and every 0.5g, and get final product.
Embodiment two
With Fractus Eriobotryae cleaning, pulverizing, drying, carry out the diafiltration extraction with the ethanol of different volumes ratio and the mixed solvent of water successively, the volume ratio of ethanol and water is 95: 5,80: 20,60: 40,40: 60; Residue after extraction water diafiltration again extracts, last waste; Merge above mixed solvent extracting solution and aqueous extract, get the full extract of Fractus Eriobotryae.
The full extract of gained is condensed into the dope of distinguishing the flavor of without alcohol, then is adjusted to 1/5th to 1/8th volumes of former full extract volume with suitable quantity of water; Then use successively petroleum ether, ethyl acetate, n-butanol extraction; Combined ethyl acetate extraction section and n-butanol extraction section are pulverized after concentrate drying, cross 80 mesh sieves, in incapsulating, and every 0.5g, and get final product.
Embodiment three
With Fractus Eriobotryae cleaning, pulverizing, drying, first water decocts to get decocting liquid; Then residue is carried out the diafiltration extraction with the ethanol of different volumes ratio and the mixed solvent of water successively, the volume ratio of ethanol and water is 40: 60,60: 40,80: 20,95: 5, abandons at last residue; Merge above decocting liquid and mixed solvent extracting solution, get the full extract of Fractus Eriobotryae.
The full extract of gained is condensed into the dope of distinguishing the flavor of without alcohol, then is adjusted to 1/5th to 1/8th volumes of former full extract volume with suitable quantity of water, then use defat with petroleum ether, concentrate drying after defat obtains Fractus Eriobotryae diuresis crude extract.Crude extract is further refining with macroporous resin, first uses the pure water eluting, removes strong polar fraction; Then use successively the alcohol-water (V/V) of 20: 80,40: 60,60: 40,80: 20,95: 5 to carry out gradient elution, merge pure water mixed solvent eluting section, concentrate drying is also pulverized, and crosses 80 mesh sieves, in incapsulating, and every 0.5g, and get final product.
Embodiment four
With Fractus Eriobotryae cleaning, pulverizing, drying, first water decocts to get decocting liquid; Then residue is carried out the diafiltration extraction with the ethanol of different volumes ratio and the mixed solvent of water successively, the volume ratio of ethanol and water is 40: 60,60: 40,80: 20,95: 5, abandons at last residue; Merge above decocting liquid and mixed solvent extracting solution, get the full extract of Fractus Eriobotryae.
The full extract of gained is condensed into the dope of distinguishing the flavor of without alcohol, then is adjusted to 1/5th to 1/8th volumes of former full extract volume with suitable quantity of water; Then use successively petroleum ether, ethyl acetate, n-butanol extraction; Combined ethyl acetate extraction section and n-butanol extraction section are pulverized after concentrate drying, cross 80 mesh sieves, in incapsulating, and every 0.5g, and get final product.
Claims (9)
1. a diuretic, it is characterized in that being made by Fractus Eriobotryae.
2. Fractus Eriobotryae according to claim 1 comprises Folium Eriobotryae peel, loquat fruit, Radix Eriobotryae.
3. the first extracting method of according to claim 1-2 described diuretic: with Fractus Eriobotryae cleaning, pulverizing, drying, extract with the organic solvent of different volumes ratio and the mixed solvent of water successively, the volume ratio of organic solvent and water is 95: 5,80: 20,60: 40,40: 60; Residue after extraction is used water extraction again, last waste; Merge above mixed solvent extracting solution and aqueous extract, get the full extract of Fractus Eriobotryae.
4. the second extracting method of according to claim 1-2 described diuretic: with Fractus Eriobotryae cleaning, pulverizing, drying, first water decocts to get decocting liquid; Then residue is extracted with the organic solvent of different volumes ratio and the mixed solvent of water successively, the volume ratio of organic solvent and water is 40: 60,60: 40,80: 20,95: 5, abandons at last residue; Merge above decocting liquid and mixed solvent extracting solution, get the full extract of Fractus Eriobotryae.
5. according to claim 3,4 described organic solvents are a kind of in methanol, ethanol or acetone.
6. according to claim 3,4 described mixed solvents extract, extracting mode is that a kind of or combination of merceration extraction, heating extraction, diafiltration extraction, reflux, extract,, supersound extraction, microwave extraction mode is used.
7. the first preparation method of according to claim 1-6 described Fractus Eriobotryae diuretic: according to claim 1-6 full extracts that make are condensed into dope without the alcohol flavor, be adjusted to again 1/5th to 1/8th volumes of former full extract volume with suitable quantity of water, then use defat with petroleum ether, concentrate drying after defat obtains Fractus Eriobotryae diuresis crude extract.Crude extract is further refining with macroporous resin, first uses the pure water eluting, removes strong polar fraction; Then use successively the alcohol-water (V/V) of 20: 80,40: 60,60: 40,80: 20,95: 5 to carry out gradient elution, merge pure water mixed solvent eluting section, make oral drugs after concentrate drying.
8. the second preparation method of according to claim 1-6 described Fractus Eriobotryae diuretic: according to claim 1-6 full extracts that make are condensed into dope without the alcohol flavor, then are adjusted to 1/5th to 1/8th volumes of former full extract volume with suitable quantity of water; Then use successively petroleum ether, ethyl acetate, n-butanol extraction; Combined ethyl acetate extraction section and n-butanol extraction section are made oral drugs after concentrate drying.
9. according to claim 7,8 described oral drugs are granule, tablet, capsule.
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| Application Number | Priority Date | Filing Date | Title |
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| CN2011103854217A CN103127266A (en) | 2011-11-29 | 2011-11-29 | Loquat-shaped cake diuretic and extraction and preparation method thereof |
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| CN2011103854217A CN103127266A (en) | 2011-11-29 | 2011-11-29 | Loquat-shaped cake diuretic and extraction and preparation method thereof |
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS55164628A (en) * | 1979-06-12 | 1980-12-22 | Yoshimi Takenaka | Powdery coffee containing medicinal herb |
| JP2004242586A (en) * | 2003-02-14 | 2004-09-02 | Eifuji:Kk | Method for producing processed food, composite food thereof or juice |
| CN1931258A (en) * | 2006-09-21 | 2007-03-21 | 福州大学 | Prepn process and use of loquat seed extract |
| CN101591607A (en) * | 2009-07-09 | 2009-12-02 | 杭州同福永酿酒有限公司 | Brewing method for loquat wine |
-
2011
- 2011-11-29 CN CN2011103854217A patent/CN103127266A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS55164628A (en) * | 1979-06-12 | 1980-12-22 | Yoshimi Takenaka | Powdery coffee containing medicinal herb |
| JP2004242586A (en) * | 2003-02-14 | 2004-09-02 | Eifuji:Kk | Method for producing processed food, composite food thereof or juice |
| CN1931258A (en) * | 2006-09-21 | 2007-03-21 | 福州大学 | Prepn process and use of loquat seed extract |
| CN101591607A (en) * | 2009-07-09 | 2009-12-02 | 杭州同福永酿酒有限公司 | Brewing method for loquat wine |
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Application publication date: 20130605 |