CN103109973A - Method for preparing lactic acid bacteria feed additive by using eleocharis tuberosa starch wastewater - Google Patents
Method for preparing lactic acid bacteria feed additive by using eleocharis tuberosa starch wastewater Download PDFInfo
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- CN103109973A CN103109973A CN2013100642009A CN201310064200A CN103109973A CN 103109973 A CN103109973 A CN 103109973A CN 2013100642009 A CN2013100642009 A CN 2013100642009A CN 201310064200 A CN201310064200 A CN 201310064200A CN 103109973 A CN103109973 A CN 103109973A
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- lactic acid
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Abstract
The invention discloses a method for preparing lactic acid bacteria feed additive by using eleocharis tuberosa starch wastewater. The method is characterized by adopting the direct lactic acid bacteria agent of which the strain is lactobacillus plantarum as the initial strain, and comprising the following step of: carrying out the two-stage amplification, wherein the amplification condition is to seal at the natural environment temperature and stay the night, the rate of the first-stage volume to the amplification proportion is 1:5000-10000, and the rate of the second-stage volume to the amplification proportion is 1:4-9; performing in-series continuous fermentation; and obtaining the lactic acid bacteria feed additive after fermenting for 10 hours. According to the invention, the raw material is easily available, heat treatment during the production is not needed, and sterilization and preservation operations are not needed, so that a large amount of energy can be saved, the cost can be reduced, discharge of wastewater is avoided, the process is environmental-friendly, and the industrial production can be smoothly achieved.
Description
Technical field
The invention belongs to feed additive field, particularly utilize the water chestnut starch wastewater to produce the method for lactobacillus feed additive.
Background technology
Lactic acid bacteria has the promotion growth of animal, improves corrupt bacteria growing in feed digestibility and biological value, inhibition enteron aisle, guarantees that animal body is healthy, reduce the even multiple effects such as use of substitute antibiotics, and the application in the aquacultures such as beasts, birds and aquatic products is more and more wider.China Ministry of Agriculture is defined in microorganism available in feed addictive 16 kinds, and wherein 11 kinds is lactic acid bacteria.The lactic acid bacteria nutritional requirement is very complicated, up to now, the preparation of the lactic acid bacteria of having reported is nearly all to utilize with glucose, peptone, yeast extract, inorganic salts, trace element, vitamin etc. are fermented for the synthetic culture medium of raw material, as Chinese patent " preparation method of the biological feed additive of lactobacillus " (patent No.: 201010122147), " a kind of feeding lactobacillus high density fermentation culture medium and fermentation process thereof " (patent No. 201110116555), Chinese patent application " a kind of high-density cultivation method of lactic acid bacteria " (application number 200710004941.2.), " a kind of high-intensity culture method for lactobacilli " (application number 200910057650), and " development of efficient lactic acid bacterium throw type leaven " Tan Huan .[J]. the Agriculture in Hunan science, 2012, (9): 102-104, 107 literary compositions such as grade have all been used said method, cultivate lactic acid bacterial count in rear liquid and all can reach 10
9more than cfu/ml, but cost of material is high, and cost of material accounts for the 30%-50% of fermentation costs, and to carry out omnidistance sterilizing to culture medium, and also want constant temperature to 36 ℃ or 42 ℃ to be cultivated simultaneously, energy resource consumption is also high, the processing of fermented waste fluid is also very complicated in addition.Accidentally with biomass material, as pineapple peel, mushroom juice and potato juice, increased the report of bacterium, [J] dairy industry science and technologies such as " research of novel lactic acid bacteria enriched medium " Du Lei, .2012,35 (1) 4-7.), except the shortcoming with the sterilizing of needs, constant temperature equal energy source consumption height, also there are the defects such as unstable, the difficult preservation of raw material sources in the method.Therefore, also do not realize the suitability for industrialized production of lactic acid bacteria in China.For realizing the large-scale production of lactic acid bacteria, in the urgent need to find a kind of raw material inexpensive, be easy to get, the new method of process low-carbon, environmental protection.
Summary of the invention
The technical problem to be solved in the present invention be to provide a kind of raw material inexpensive, be easy to get, the lactic acid bacteria production method of process low-carbon, environmental protection.
The present invention solves the problems of the technologies described above with following technical scheme:
Processing step is:
1), select commercialization to deliver directly agent of lactic acid bacteria, bacterial classification is Lactobacillus plantarum (Lactobacillus plantarum), by GB4789.35-2010 " check of food security national standard food microbiological analysis lactic acid bacteria " method, detected, lactic acid bacterial count is not less than 10
11cfu/g is qualified;
2), the bacterial classification one-level is amplified: in the volumetric ratio access water chestnut starch wastewater by bacterial classification by 1 ︰ 5000～10000, at the natural environment temperature, sealing is spent the night, and is cultured to the pH value lower than 4.0, obtains the one-level kind;
3) the bacterial classification secondary amplifies: in the volumetric ratio access water chestnut starch wastewater by the one-level kind by 1 ︰ 4～9, at the natural environment temperature, sealing is spent the night, and is cultured to the pH value lower than 4.0, obtains the secondary kind;
4) the water chestnut starch wastewater continuously ferments: the secondary kind is all pumped in large tank, large tankage size is 6 times-10 times of secondary kind total capacity, then pump into waste water, waste water is filled it up with rear next tank that automatically flows into, totally 6-10 the series connection large tanks, time to last tank is no less than 10 hours, and pH is lower than 4.0;
5) obtain the lactobacillus feed additive finished product: last tank fermentation waste water out, the Lactobacillus plantarum counting is not less than 10
9cfu/ml, but direct packaging in airtight container as lactobacillus feed additive, can be widely used in the beasts, birds and aquatic products cultivation.
The present invention has following advantage:
1) cost of material is cheap, due to what utilize, is the water chestnut starch wastewater, so the cost of nutrient solution is almost nil.
2) raw material is easy to get, and produces season, and a large amount of discharge of wastewater are arranged every day, and with the starch production scale calculating of 2000 tons/season, every day, 120 tons of waste water of discharge can be disposed in time.
3) process low-carbon, whole production process is without any need for heat treatment, and both not sterilizings, be not incubated again, can save mass energy.
4) technique environmental protection, waste water all becomes lactobacillus suspension, and waste water is almost zero-emission.
The specific embodiment
The soluble solid that the water chestnut starch wastewater contains 1.5% left and right, experiment records and wherein contains 0.4% total reducing sugar, and 0.35% protein, also have a large amount of vitamin and mineral matter in addition, nutritious.The present invention be take the water chestnut starch wastewater as raw material, selects and delivers directly agent of lactic acid bacteria, after two-stage is amplified, pumps into large tank and is continuously fermented, and after 10 hours, the results lactic acid bacterial count reaches 10
9the high concentration lactobacillus suspension of cfu/ml.
Following examples are to further describe of the present invention, but the present invention is not limited to following examples.It is example that embodiment be take the plant chimney stalk of 2000 tons of water chestnut starch scales of per season.
Embodiment 1
Get 20g and deliver directly agent of lactic acid bacteria, join in the Plastic Drum that 100L water chestnut starch wastewater is housed, natural environment temperature lower seal spends the night; In the plastic tank that the capacity of pouring into again is 1000L, add waste water to 1000L, natural environment temperature lower seal spends the night; Afterwards, all pump in the plastic tank of first 6000L capacity, then pass into continuously waste water, flow velocity 5000L/ hour, enter next series can after the waste water canful, totally 10 tank series connection; 10 tanks all are full of about 12h, use the lactobacillus feed additive of the direct filling gained of 25L Plastic Drum from the outlet of last tank.Can utilize 120 tons of waste water productions every day is 10 with the lactic acid bacteria density of amount
9the lactobacillus feed additive of cfu/ml.
Embodiment 2
Get 20g and deliver directly agent of lactic acid bacteria, join in the Plastic Drum that 200L water chestnut starch wastewater is housed, natural environment temperature lower seal spends the night; In the plastic tank that the capacity of pouring into again is 1000L, add waste water to 1000L, natural environment temperature lower seal spends the night; Afterwards, all pump in the plastic tank of 10000L capacity, then pass into continuously waste water, flow velocity 5000L/ hour, enter next series can after the waste water canful, totally 6 tank series connection; Waste water naturally continuously ferments after 12h in 6 tanks, from the 6th tank, out directly is filled into the 25L Plastic Drum, obtains lactobacillus feed additive.Can utilize 120 tons of waste water every day, the lactic acid bacteria density of producing with amount is 10
9the lactobacillus feed additive of cfu/ml.
Embodiment 3
Get 20g and deliver directly agent of lactic acid bacteria, join in the Plastic Drum that 100L water chestnut starch wastewater is housed, natural environment temperature lower seal spends the night; In the plastic tank that the capacity of pouring into again is 1000L, add waste water to 1000L, natural environment temperature lower seal spends the night; Afterwards, all pump in the plastic tank of 6000L capacity, then pass into continuously waste water, flow velocity 5000L/ hour, enter next series can after the waste water canful, and wherein front 5 tanks are the 6000L tank, and rear 3 tanks are the 10000L tank, totally 8 tank series connection; Waste water in 8 tanks after spontaneous fermentation 11h, from the 8th tank direct filling 25L Plastic Drum out, obtains lactobacillus feed additive.Can utilize 120 tons of waste water every day, the lactic acid bacteria density of producing with amount is 10
9the lactobacillus feed additive of cfu/ml.
Claims (1)
1. a method of utilizing the water chestnut starch wastewater to produce lactobacillus feed additive, processing step is:
1) select commercialization to deliver directly agent of lactic acid bacteria, bacterial classification is Lactobacillus plantarum, by GB GB4789.35-2010 method, is detected, and lactic acid bacterial count is not less than 10
11cfu/g is qualified;
2) the bacterial classification one-level is amplified: in the volumetric ratio access water chestnut starch wastewater by bacterial classification by 1 ︰ 5000～10000, at the natural environment temperature, sealing is spent the night, and is cultured to the pH value lower than 4.0, obtains the one-level kind;
3) the bacterial classification secondary amplifies: in the volumetric ratio access water chestnut starch wastewater by the one-level kind by 1 ︰ 4～9, at the natural environment temperature, sealing is spent the night, and is cultured to the pH value lower than 4.0, obtains the secondary kind;
4) the water chestnut starch wastewater continuously ferments: the secondary kind is all pumped in large tank, large tankage size is 6 times-10 times of secondary kind total measurement (volume), then pump into waste water, waste water is filled it up with rear next tank that automatically flows into, totally 6-10 the series connection large tanks, time to last tank is no less than 10 hours, and pH is lower than 4.0;
5) obtain the lactobacillus feed additive finished product: last tank fermentation waste water out, the Lactobacillus plantarum counting is not less than 10
9cfu/ml, but direct packaging in airtight container as lactobacillus feed additive, be widely used in the beasts, birds and aquatic products cultivation.
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| CN2013100642009A CN103109973A (en) | 2013-02-28 | 2013-02-28 | Method for preparing lactic acid bacteria feed additive by using eleocharis tuberosa starch wastewater |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108277183A (en) * | 2018-03-16 | 2018-07-13 | 中科美大（北京）生态环境工程有限公司 | A method of preparing animal probiotics using potato starch wastewater |
| CN108669289A (en) * | 2018-04-09 | 2018-10-19 | 广东农工商职业技术学院(农业部华南农垦干部培训中心) | A kind of technique preparing feed addictive |
Citations (4)
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| JP2000125776A (en) * | 1998-10-20 | 2000-05-09 | Moriyuki Sakamoto | Liquid feed using wastewater of rice washing and its production |
| CN101822310A (en) * | 2010-05-06 | 2010-09-08 | 江南大学 | Method for cultivating lactobacillus and producing live lactobacillus feed by utilizing scum |
| CN102210383A (en) * | 2011-04-08 | 2011-10-12 | 甘肃圣大方舟马铃薯变性淀粉有限公司 | Method for preparing mycoprotein feed from waste water and waste residues in processing process of potatoes |
| CN102815795A (en) * | 2012-09-12 | 2012-12-12 | 广西民族大学 | Method for processing starch wastewater as well as product and application thereof |
-
2013
- 2013-02-28 CN CN2013100642009A patent/CN103109973A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2000125776A (en) * | 1998-10-20 | 2000-05-09 | Moriyuki Sakamoto | Liquid feed using wastewater of rice washing and its production |
| CN101822310A (en) * | 2010-05-06 | 2010-09-08 | 江南大学 | Method for cultivating lactobacillus and producing live lactobacillus feed by utilizing scum |
| CN102210383A (en) * | 2011-04-08 | 2011-10-12 | 甘肃圣大方舟马铃薯变性淀粉有限公司 | Method for preparing mycoprotein feed from waste water and waste residues in processing process of potatoes |
| CN102815795A (en) * | 2012-09-12 | 2012-12-12 | 广西民族大学 | Method for processing starch wastewater as well as product and application thereof |
Non-Patent Citations (1)
| Title |
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| 龚丽，等: "马蹄淀粉废渣的处理和利用", 《现代农业装备》, no. 4, 31 December 2003 (2003-12-31) * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108277183A (en) * | 2018-03-16 | 2018-07-13 | 中科美大（北京）生态环境工程有限公司 | A method of preparing animal probiotics using potato starch wastewater |
| CN108669289A (en) * | 2018-04-09 | 2018-10-19 | 广东农工商职业技术学院(农业部华南农垦干部培训中心) | A kind of technique preparing feed addictive |
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Application publication date: 20130522 |