CN103102018B - Compound water ecology restoration agent and preparation method thereof - Google Patents
Compound water ecology restoration agent and preparation method thereof Download PDFInfo
- Publication number
- CN103102018B CN103102018B CN201310049073.5A CN201310049073A CN103102018B CN 103102018 B CN103102018 B CN 103102018B CN 201310049073 A CN201310049073 A CN 201310049073A CN 103102018 B CN103102018 B CN 103102018B
- Authority
- CN
- China
- Prior art keywords
- oxidizing bacteria
- preparation
- water
- pseudomonas
- compound water
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Landscapes
- Purification Treatments By Anaerobic Or Anaerobic And Aerobic Bacteria Or Animals (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention relates to a microorganism inoculant, and especially relates to a compound water ecology restoration agent and a preparation method. The compound water ecology restoration agent is a composition composed of 48-55% of ammonia oxidizing bacteria, 33-40% of nitrogen oxidizing bacteria and 5-15% of denitrifying bacteria. The restoration agent has good effects on water environment ecological treatment technology, urban landscape water treatment, ecological lake comprehensive treatment, river water environment comprehensive treatment, rural sewage treatment, aquaculture circulating water treatment and the like.
Description
Technical field
The present invention relates to a kind of microbial bacteria preparation, relate in particular to a kind of Compound Water restoration of the ecosystem preparation and preparation method thereof.
Background technology
At present, pollution and the situation of preventing and treating of the aspect such as the urban landscape water of China, ecological lake, river water burst environment, domestic sewage in rural areas by using, aquiculture recycled water allow of no optimist.Due to the reinforcement of urban construction, the increase in demand of urban landscape water, requires further aggravation to the improvement of its water surrounding.At present China is for the improvement of these water surroundings, and what often adopt is water treatment device, and these devices not only takes electricity, also must be in the time administering competence exertion effect, once stop using, water surrounding still can continue deterioration.
Summary of the invention
For above deficiency, the invention provides a kind of activity strong, can continue to repair and change the microbial bacteria preparation of water surrounding, and provide the method for preparing said preparation.
The present invention achieves the above object by following scheme:
A kind of Compound Water restoration of the ecosystem preparation, the composition being formed by ammonia oxidizing bacteria, nitrogen oxidizing bacteria and denitrifying bacterium, the weight percent of wherein said each component is ammonia oxidizing bacteria 48-55%, nitrogen oxidizing bacteria 33-40%, denitrifying bacterium 5-15%.
The weight percent of described each component is ammonia oxidizing bacteria 53%, nitrogen oxidizing bacteria 37% and denitrifying bacterium 10%,
Described ammonia oxidizing bacteria comprises one or more in Nitromonas, Nitrosococcus.
Wherein Nitromonas comprises
nitrosomonas aestuarii, N. communis, N. cryotolerans, N. europaea, N. eutropha, N. halophila, N. marina, N. nitrosa, N. oligotropha, N. ureae, N. sp.deng, Nitrosococcus comprises
nitrosococcus oceanus, Nitrosococcus oceani, N. halophilusdeng.
Described nitrogen oxidizing bacteria comprises one or more in nitric acid Pseudomonas, nitrated spiral Pseudomonas.
Wherein, nitric acid Pseudomonas comprises Vickers bacterium nitrobacter, Vickers nitric acid bacillus etc., and nitrated spiral Pseudomonas comprises
nitrospira marina, N. moscoviensis, N. sp.deng.
Described denitrifying bacterium comprises Pseudomonas aeruginosa
p. Aeruginosa, pseudomonas syringae
p. syringae, pseudomonas putida
p. putida, Pseudomonas fluorescens
p. fluorescens.in one or more.
Prepare a method for above-mentioned Compound Water restoration of the ecosystem preparation, comprise the each bacterial classification in each component described in inoculation culture, the rear spraying of having fermented is dry.
Natural nitrated-denitrification flora increases very slow, just be difficult to recover once destroyed, and in preparation for repairing of the present invention, contain through purify and cultivate nitrated-denitrifying bacterium flora, ammonia oxidizing bacteria and nitrogen oxidizing bacteria all belong to nitrobacteria, bring into play different functions at different nitrifying processes respectively, when these microorganisms enter after polluted water body, by promoting the circulation of nitrogen in water body-biology-atmosphere three-phase, thereby unnecessary nitrogen is migrated out from water body, or transfer in atmosphere with the form of gas, or be stored in plant with organic form, microorganism, in the body of high hydrocoles etc.Nitrated principle is reacted as shown in Figure 1, specifically, comprising:
1) nitrated NH
3+ CO
2+ 1.5O
2+ ammonia oxidizing bacteria → NO
2 -+ H
2o+H
+
NO
2 -+ CO
2+ 0.5O
2+ nitrogen oxidizing bacteria → NO
3 -
NH
3+ O
2+ ammonia oxidizing bacteria → NO
2 -+ 3H
++ 2e
-
NO
2 -+H
2O→NO
3 -+2H
++2e
-
1) denitrification 2NO
3 -+ 4H
++ 4e
-→ 2NO
2 -+ 2H
2o
2NO
2 -+4H
++2e
-→2NO+2H
2O
2NO+2H
++2e-→N
2O+H
2O
N
2O+2H
++2e
-→N
2+H
2O。
In preparation for repairing of the present invention each microbial profile in water body after, taking the nutritive element that is rich in water as food, raised growth breeding, accelerates to consume nutritive salt in water, and water body is purified.They can long-term existence in water body, water body has been become to huge " biological reaction tank ", in the time that unpredictable pollution of area source occurs, the Eutrophication materials that flows into water body is decomposed by these natural Institute of Micro-biology, they digest these " pollutents " by the effect of self, make pollution of area source controlled.
By this preparation for repairing, waterplant, animal acting in conjunction, promote the indigenous microorganism growth and breeding of occurring in nature, for polluted water body is created an environment that can complete smoothly pollutent natural degradation function.Cultivate by original position, increase microorganism active, by microbial life activity, to water pollutant shift, conversion and Degradation, thereby promote water body to develop to the aerobic aquatic ecosystem of cleaning, food chain is extended and stable system, improve impact resistance, recover gradually the unify self-purification function of water body of aquatic ecosystem.
Apply the present invention to carry out in aquaculture and landscape pond compliance test result test.Certain Fanyu raiser's more than 100 mu of grass carp cultivating pool, before use, often there are various bacteriosises, bottom material of pond for breeding blackout, transparency 20cm, in water, ammonia nitrogen and nitrite are respectively 1.2mg/L and 0.3mg/L, after 3 days of use embodiment 1, measure, ammonia nitrogen and nitrate are reduced to respectively 0.2mg/L and 0.05mg/L, and transparency reaches 35cm.After 1 month of use embodiment 1, the substrate blackout part in pond starts to bleach, and mud has part defeated and dispersed.10 days in advance results fishes of this raiser, and before when untapped preparation for repairing the specification of 10 rear results fishes similar, there is not any bacteriosis to receipts this pond of fish always.
This preparation for repairing is in water surrounding ecological management technology, urban look water treatment, and the comprehensive regulation of ecological lake, river water burst Water Environmental Synthesis Dredge, rural domestic sewage treatment, the aspects such as aquiculture recycled water processing have all obtained extraordinary effect.
Brief description of the drawings
Fig. 1 is the nitrated principle reaction schematic diagram of the denitrifying bacterium of preparation for repairing of the present invention.
Embodiment
Below in conjunction with specific embodiment, the present invention is further described.
Embodiment 1:
A kind of Compound Water restoration of the ecosystem preparation:
nitrosomonas aestuarii53%, Vickers bacterium nitrobacter 37% and Pseudomonas aeruginosa
p. Aeruginosa10%.
Preparation process: occurring in nature gathers bacterium, line separation of bacterial, the kind of biochemical identification bacterium, filters out each bacterial classification, and shaking table is cultivated, and each bacterial classification is preserved in lyophilize.Each bacterial classification renewed vaccination is cultivated, shaking table one-level amplification culture, secondary amplifies bacterial classification, continues enlarged culturing bacterial classification three times, and the rear spray dried prod that fermented, makes finished product packing.
Embodiment 2:
A kind of Compound Water restoration of the ecosystem preparation:
nitrosococcus oceanus48%, Vickers nitric acid bacillus 37% and pseudomonas syringae
p. syringae15%.
Preparation process: occurring in nature gathers bacterium, line separation of bacterial, the kind of biochemical identification bacterium, filters out each bacterial classification, and shaking table is cultivated, and each bacterial classification is preserved in lyophilize.Each bacterial classification renewed vaccination is cultivated, shaking table one-level amplification culture, secondary amplifies bacterial classification, continues enlarged culturing bacterial classification three times, and the rear spray dried prod that fermented, makes finished product packing.
Embodiment 3:
A kind of Compound Water restoration of the ecosystem preparation:
n. communis55%,
nitrospira marina40% and pseudomonas putida
p. putida5%.
Preparation process: occurring in nature gathers bacterium, line separation of bacterial, the kind of biochemical identification bacterium, filters out each bacterial classification, and shaking table is cultivated, and each bacterial classification is preserved in lyophilize.Each bacterial classification renewed vaccination is cultivated, shaking table one-level amplification culture, secondary amplifies bacterial classification, continues enlarged culturing bacterial classification three times, and the rear spray dried prod that fermented, makes finished product packing.
Embodiment 4:
A kind of Compound Water restoration of the ecosystem preparation:
n. halophilus52%,
n. moscoviensis33% and Pseudomonas fluorescens
p. fluorescens.15%.
Embodiment 5:
A kind of Compound Water restoration of the ecosystem preparation:
n. halophila54%,
n. sp.38% and pseudomonas syringae
p. syringae8%.
Embodiment 6:
A kind of Compound Water restoration of the ecosystem preparation:
n. oligotropha50%,
nitrospira marina36% and Pseudomonas fluorescens
p. fluorescens.14%.
Embodiment 7:
A kind of Compound Water restoration of the ecosystem preparation:
n. europaea49%, Vickers bacterium nitrobacter 39% and Pseudomonas aeruginosa
p. Aeruginosa12%.
Embodiment 8:
A kind of Compound Water restoration of the ecosystem preparation:
nitrosococcus oceani51%, Vickers nitric acid bacillus 34% and pseudomonas putida
p. putida15%.
The above; it is only preferably specific embodiment of the present invention; but protection scope of the present invention is not limited to this; any be familiar with those skilled in the art the present invention disclose technical scope in; be equal to replacement or changed according to technical scheme of the present invention and design thereof, all should be encompassed in protection scope of the present invention.
Claims (3)
1. a Compound Water restoration of the ecosystem preparation, it is characterized in that, by ammonia oxidizing bacteria, the composition of nitrogen oxidizing bacteria and denitrifying bacterium composition, the weight percent of wherein said each component is ammonia oxidizing bacteria 48-55%, nitrogen oxidizing bacteria 33-40%, denitrifying bacterium 5-15%, described ammonia oxidizing bacteria comprises Nitromonas, one or more in Nitrosococcus, described nitrogen oxidizing bacteria comprises nitric acid Pseudomonas, one or more in nitrated spiral Pseudomonas, described denitrifying bacterium comprises pseudomonas putida, Pseudomonas aeruginosa, pseudomonas syringae, one or more in Pseudomonas fluorescens.
2. a kind of Compound Water restoration of the ecosystem preparation as claimed in claim 1, is characterized in that, the weight percent of described each component is ammonia oxidizing bacteria 53%, nitrogen oxidizing bacteria 37% and denitrifying bacterium 10%.
3. a method of preparing Compound Water restoration of the ecosystem preparation in claim 1-2, is characterized in that, comprises the each bacterial classification in each component described in inoculation culture, and the rear spraying of having fermented is dry.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310049073.5A CN103102018B (en) | 2013-02-07 | 2013-02-07 | Compound water ecology restoration agent and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310049073.5A CN103102018B (en) | 2013-02-07 | 2013-02-07 | Compound water ecology restoration agent and preparation method thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103102018A CN103102018A (en) | 2013-05-15 |
| CN103102018B true CN103102018B (en) | 2014-12-10 |
Family
ID=48310262
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310049073.5A Active CN103102018B (en) | 2013-02-07 | 2013-02-07 | Compound water ecology restoration agent and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103102018B (en) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103484413A (en) * | 2013-10-14 | 2014-01-01 | 青岛蔚蓝生物集团有限公司 | Pseudomonas putida strain and application thereof |
| CN105540873B (en) * | 2016-01-30 | 2018-02-13 | 昆山华苏原料药有限公司 | A kind of method for handling tetracycline antibiotics waste water |
| KR102652219B1 (en) * | 2021-09-03 | 2024-03-29 | 주식회사 에코씨디아이 | Novel Pseudomonas putida strain having denitrification activity and use of the same |
Family Cites Families (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1205135C (en) * | 2003-05-30 | 2005-06-08 | 成都海发（集团）股份有限公司 | Technique for treating wastewater by using microorganism |
| CN101082029A (en) * | 2006-06-01 | 2007-12-05 | 上海泓宝绿色水产科技发展有限公司 | Preparation of water purification bacterium and method for restoring aquaculture environment |
| JP2008017713A (en) * | 2006-07-10 | 2008-01-31 | Japan Science & Technology Agency | Method for enrichment culture of ammonia-oxidizing bacterium community and denitrifying bacterium community |
| CN101033450A (en) * | 2007-01-18 | 2007-09-12 | 赵志龙 | Method of producing composite and highly effective microorganism preparation for waste water treatment |
| CN101306903B (en) * | 2007-05-18 | 2011-09-21 | 中国石油化工股份有限公司 | Biochemical treatment process for high-concentration ammonia-nitrogen-containing waste water |
| TWI386374B (en) * | 2008-12-02 | 2013-02-21 | Ind Tech Res Inst | System and method for treating waste water containing ammonia |
| CN101434907B (en) * | 2008-12-18 | 2010-12-08 | 天津大学 | Microbial preparation for processing refuse leachate and preparation |
| CN101823812A (en) * | 2010-03-16 | 2010-09-08 | 福建科佳奇迈生物工程有限公司 | Method for treating pollution biogas slurry of poultry and livestock breeding |
| CN102718327B (en) * | 2012-07-05 | 2013-11-06 | 浙江皇冠科技有限公司 | Nano-biological water body remediation agent for aquaculture and preparation method thereof |
-
2013
- 2013-02-07 CN CN201310049073.5A patent/CN103102018B/en active Active
Non-Patent Citations (2)
| Title |
|---|
| 硝化细菌分子生态学研究进展;马英等;《中国水产科学》;20070915(第05期);第1段、第1节第1段、摘要、第1.1节、1.2节 * |
| 马英等.硝化细菌分子生态学研究进展.《中国水产科学》.2007,(第05期), * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN103102018A (en) | 2013-05-15 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104803570B (en) | Ecological restoration and purification method for landscape water environment | |
| CN101823859B (en) | Light ecological concrete brick and preparation method thereof | |
| Todd et al. | Ecological design applied | |
| US20190045756A1 (en) | Dark-bright integrated greenhouse system in intensive recirculating eco-aquaculture and aquaculture method | |
| CN101475265B (en) | Water purification method for circulating water industrialized aquiculture system and complex bacterial agent thereof | |
| González-Fernández et al. | Nitrogen transformations under different conditions in open ponds by means of microalgae–bacteria consortium treating pig slurry | |
| Browdy et al. | Biofloc‐based aquaculture systems | |
| CN101607809B (en) | Microorganism concrete brick and preparation method thereof | |
| CN101885543A (en) | Method for effectively treating sewage by using both microbial cells and enzyme preparations | |
| CN104862257A (en) | Enrichment culture method of indigenous nitrogen transforming microorganism and application of same to treatment of water ammonia-nitrogen pollution | |
| CN1693236A (en) | Process for water body repairing by microorganism film biologic chinampa for large type water body pollution treatment | |
| CN109879445A (en) | A kind of river water quality original position method for improving and system | |
| CN103102018B (en) | Compound water ecology restoration agent and preparation method thereof | |
| CN109052834A (en) | A kind of administering method of eutrophication water | |
| CN101701197B (en) | Novel microorganism flora mixture and mixed nutrient medium thereof | |
| CN109179936B (en) | In-situ treatment method suitable for ecological restoration of light-pollution clean pond | |
| CN107400648A (en) | A kind of complex microorganism combines microbial inoculum, its preparation method and application, a kind of comprehensive processing method of black-odor riverway | |
| CN205088059U (en) | Rural low contaminated water deep purification system | |
| CN109081447A (en) | Method for removing nitrogen and phosphorus in culture wastewater by combining chlorella, acinetobacter and pseudomonas | |
| CN105524860A (en) | Culture method of fungicide for water purification | |
| CN109055252A (en) | Heterotrophic nitrification-aerobic denitrification composite microbial preparation and preparation method thereof | |
| CN103667109A (en) | Rhodobacter capsulatus and application thereof | |
| CN104120099B (en) | The cultural method of anaerobic ammonium oxidizing bacteria in the substrate of a kind of artificial swamp | |
| CN105036344A (en) | Method for biologically purifying aquaculture water body | |
| CN105309371A (en) | A pig and fish three-dimensional combined culture waste water internal circulation method |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| TR01 | Transfer of patent right | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20201221 Address after: 510700 room 501-8, 25 yayingshi Road, Huangpu District, Guangzhou City, Guangdong Province Patentee after: Guangdong litejing disinfection products Co.,Ltd. Address before: 510370 room 8036, 109 Huanhua Road, Liwan District, Guangzhou City, Guangdong Province Patentee before: GUANGZHOU DAYU ENVIRONMENTAL PROTECTION TECHNOLOGY Co.,Ltd. |