CN103099788A - Traditional Chinese medicine composite decoration from powder, preparation and decoction methods and dosage relation thereof - Google Patents
Traditional Chinese medicine composite decoration from powder, preparation and decoction methods and dosage relation thereof Download PDFInfo
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Abstract
The invention provides a traditional Chinese medicine composite decoration from powder. The particle size ranges of the Chinese medicine decoction piece are as follows: 1) the particle sizes of medicinal materials of roots, stems, seeds and fruits are 10-100 meshes; 2) the particle sizes of the medicinal materials of floral leaf and grass are 10-50 meshes; 3) the animal medicaments are 10-100 meshes; and/or 4) ore shellfish shell medicaments are 60-200 meshes. A preparation method and a decoction method of the traditional Chinese medicine composite decoration from powder, and the relation between the application dosage of the traditional Chinese medicine composite decoration from powder and the application dosage of traditional Chinese medicine decoction pieces with the same ingredients are also provided by the invention. The extraction rate of effective ingredients of the traditional Chinese medicine decoction pieces is improved; the dosage of the traditional Chinese medicine decoction piece is reduced; the availability and the curative effect of the medicament are improved; the difference of the different particle sizes of particles and traditional decoction pieces is evaluated by extract indexes and pharmacological indexes; and consistent pesticide effects are ensured when the quantitative use is determined, so as to reduce the bulk pharmaceutical chemicals and save resources.
Description
Technical field
The invention belongs to the field of Chinese medicines, relate to a kind of Chinese medicine decoction made from powder particulate composition and preparation method thereof and decocting method, the invention still further relates to the relation between the using dosage of the prepared slices of Chinese crude drugs of the using dosage of Chinese medicine decoction made from powder particulate composition and identical component.
Background technology
In recent years, due to the scarcity of Chinese material medicine resource, the medicine valency skyrockets, and this original dosage form decocting method medical material consumption of decoction is large, effective ingredient is difficult for stripping, and this has become the obstacle of Development of Chinese Medicine on some degree.Present Chinese medicine decoction mostly is the prepared slices of Chinese crude drugs and directly decocts after formula, and effective ingredient can not fully leach in decoction process, and the decoction pieces of different medical materials is different because of size, material, accurately divided dose.
The Chinese medicine decoction made from powder is that Chinese medicine powder is broken into the coarse granule slightly larger than powder, boils with decocting, and a kind of dosage form that the extracting juice that removes slag is taken is a kind of modification of ancient Chinese medicine decoction.The Chinese medicine decoction made from powder is from the different of decoction: 1. decoction refer to common pharmaceutical decocting piece directly with decoct or soak after the liquid dosage form that the method for extracting juice makes that removes slag; 2. the Chinese medicine decoction made from powder is the prepared slices of Chinese crude drugs to be ground into coarse powder be mixed with compound recipe according to clinical prescription again, remove slag after decoction or immersion and take, or the dosage form of directly taking.The Chinese crude drug overwhelming majority is animal and plant, be the former medicine with cell tissue, small part is mineral, metal, and the leaching of ingredient refers to that the solvent such as water enters its effective ingredient of dissolving after cell tissue, through diffusing into solvent, behave and drink and bring into play therapeutic effect.
Usually the leaching of effective ingredient will be conducive to after pulverizing medicinal materials due to the surface area increase.But in actual applications, the meticulous meeting of pulverizing medicinal materials causes absorption affinity to improve, the difficult dispersion, may cause the leaching of active ingredient, paste-forming rate to descend, simultaneously for decocting rear medicine juice and bringing difficulty separating of medicinal residues, make and sneak into a large amount of useless medicinal residues in medicine juice, cause and take inconvenience.Therefore, as foundation, the grinding particle size of certain limit is determined in screening with objective indicator, not only improves drug effect but also facilitate the Chinese medicine decoction made from powder that uses to reach, and becomes the problem demanding prompt solution of this dosage form of development Chinese medicine decoction made from powder.Simultaneously, should be take pharmacodynamic index as the basis, the dosage laws of use of clear and definite decoction made from powder and traditional decoction pieces instructs clinical application.
Summary of the invention
One object of the present invention is the deficiency for existing Chinese medicine decoction and Chinese medicinal granule; a kind of requirement that can satisfy accurate ingredients is provided; meet again the Chinese medicine traditional theory fully, and effectively reduce the Chinese crude drug loss, the Chinese medicine decoction made from powder particulate composition of protection Chinese material medicine resource.
Another object of the present invention is to provide the preparation method of above-mentioned Chinese medicine decoction made from powder particulate composition.
Another purpose of the present invention is to provide the decocting method of above-mentioned Chinese medicine decoction made from powder particulate composition.
Also another purpose of the present invention is to provide the relation between the using dosage of the prepared slices of Chinese crude drugs of the using dosage of above-mentioned Chinese medicine decoction made from powder particulate composition and identical component, and the dosage laws of use with clear and definite decoction made from powder and traditional decoction pieces instructs clinical application.
For above-mentioned purpose, technical scheme of the present invention is as follows:
On the one hand, the invention provides a kind of Chinese medicine decoction made from powder particulate composition, in described decoction made from powder particulate composition, the particle size range of the prepared slices of Chinese crude drugs is:
1) root, stem, Seed-fruit Chinese drugs are 10 ~ 100 orders, are preferably 20 orders;
2) floral leaf, careless class medical material are 10 ~ 50 orders, are preferably 20 orders;
3) animal drugs is 10 ~ 100 orders, is preferably 20 orders; And/or
4) Ore shellfish Class A medicine is 60 ~ 200 orders, is preferably 100 orders.
Preferably, described, stem, Seed-fruit Chinese drugs are selected from: Radix Notoginseng, Radix Puerariae, Rhizoma Coptidis, the Radix Astragali, Radix Salviae Miltiorrhizae, Ramulus Cinnamomi, Semen Nelumbinis, Fructus Corni, Hericium erinaceus (Bull. Ex Fr.) Pers., Radix Rehmanniae Preparata, Radix Angelicae Sinensis, the Radix Paeoniae Alba, Rhizoma Chuanxiong, Radix Ginseng, Radix Ophiopogonis, Radix Glycyrrhizae, Radix Rehmanniae, Radix Et Rhizoma Rhei, the Rhizoma Pinelliae, Cortex Lycii, Radix Gentianae Macrophyllae, the Rhizoma Anemarrhenae, Caulis Akebiae, Rhizoma Typhonii, Fructus Schisandrae Chinensis, Fructus Gardeniae, Fructus Jujubae and Semen Armeniacae Amarum;
Described floral leaf, careless class medical material are selected from: Herba Hedyotidis Diffusae, Flos Lonicerae, Folium Perillae, Flos Inulae, Folium Bambusae, Herba Ephedrae and Herba Artemisiae Scopariae;
Described animal drugs is selected from: Pheretima, Placenta Hominis, Hirudo, Serpentis, Bombyx Batryticatus and Scorpio; And/or
Described Ore shellfish Class A medicine is selected from: Carapax Et Plastrum Testudinis, Talcum, Concha Ostreae, Gypsum Fibrosum, Haematitum and Carapax Trionycis.
Preferably, described decoction made from powder particulate composition becomes the fractional pack of 1 ~ 10 gram or the large of 500 ~ 1000 grams to pack with non-woven fabrics, paper bag or plastic bag packaging; The Chinese medicine decoction made from powder particulate composition that contains 1 gram, 2 grams, 3 grams, 5 grams, 6 grams or 10 grams in preferred single fractional pack.
On the other hand, the invention provides the preparation method of described Chinese medicine decoction made from powder particulate composition, described method comprises that the prepared slices of Chinese crude drugs with described each composition medical material are crushed to above-mentioned particle size range.
Preferably, described method comprises and described Chinese medicine decoction made from powder particulate composition is become 1 ~ 10 gram by clinical usual amounts with non-woven fabrics, paper bag or plastic bag packaging, preferred 1 gram, 2 grams, 3 grams, 5 grams, 6 grams or 10 grams.
Preferably, described decoction made from powder particulate composition is packaged into the large packing of 500 ~ 1000 grams, is placed in respectively special-purpose particle packaging machine hopper, hybrid packed according to doctor's clinical formulation setting amount of taking, form the decoction made from powder particulate composition.
Preferably, described fractional pack decoction made from powder granule is taken appropriate amount according to doctor's clinical formulation, mix before use or directly drop into decocting.
Another aspect the invention provides the decocting method of described Chinese medicine decoction made from powder particulate composition, comprises the following steps:
1) get described decoction made from powder particulate composition and pour container into or with the non-woven bag parcel, add cold water soak, then straight fire boils, and with slow fire boiling, leaches medicinal liquid after boiling;
2) add entry in step 1) gained medicinal residues, straight fire boils, and uses slow fire boiling instead after boiling, and leaches medicinal liquid;
3) with step 1) and step 2) merging of gained medicinal liquid, and get final product.
Preferably, be incorporated as the cold water soak of 8 ~ 12 times of weight of described decoction made from powder particulate composition weight in described step 1).
Preferably, soak 20 ~ 60min after adding cold water in described step 1).
Preferably, in described step 1), the slow fire decocting time is 20 ~ 40min.
Preferably, be incorporated as the water of 6 ~ 10 times of weight of decoction made from powder particle weight described step 2).
Preferably, described step 2), the slow fire decocting time is 10 ~ 30min.
Also on the other hand, the invention provides the relation between the using dosage of the prepared slices of Chinese crude drugs of the using dosage of above-mentioned Chinese medicine decoction made from powder particulate composition and identical component, the using dosage of wherein said Chinese medicine decoction made from powder particulate composition is 30 % by weight ~ 40 % by weight of using dosage of the prepared slices of Chinese crude drugs of identical component; Preferably, the using dosage of described Chinese medicine decoction made from powder particulate composition be identical component the prepared slices of Chinese crude drugs using dosage 1/3.
Chinese medicine decoction made from powder particulate composition of the present invention, because the prepared slices of Chinese crude drugs have been carried out appropriate pulverizing, be conducive to accurate allotment, effectively guarantee accuracy and the convenience of prepared slices of Chinese crude drugs formula, improve on the decrease the extraction ratio of prepared slices of Chinese crude drugs effective ingredient when medicine decoction pieces usage quantity, improved availability and the curative effect of medicine.The invention provides granular size and the decocting method of different pulverizing medicinal materials, guaranteed the prepared slices of Chinese crude drugs in the ingredients process accuracy and decoction process in the leaching of effective ingredient, making the Chinese medicine decoction made from powder use has the method to follow, and reduces the waste of natural resources of Chinese medicinal materials.Estimated the difference of different grain size decoction made from powder granule and traditional decoction pieces by extractum index and pharmacology index, when clear and definite its use is quantitative, guaranteed that drug effect is consistent, used thereby reduce medical material, economized on resources.
The specific embodiment
Below in conjunction with following embodiment, the present invention is done further and describe in detail.
Embodiment 1
The method of preparation and use of Radix Notoginseng decoction made from powder granule
1) get the Chinese medicine notoginseng decoction piece, cross respectively 10,20,50,100 mesh sieves after pulverizing, get four kinds of Radix Notoginseng decoction made from powder granules;
2) get step 1) described Radix Notoginseng decoction made from powder granule, be packaged into respectively 1g, 2g, 3g, 5g, 6g, 10g with paper bag or plastic bag, namely get prepared slices of Chinese crude drugs decoction made from powder formula fractional pack powder particles.
Getting when 3) using needs the Radix Notoginseng of dosage decoction made from powder granule, opens plastic bag and pours container into, adds 12 times of amount cold water soak 20min, and straight fire boils, and boils rear slow fire boiling 10min, leaches medicinal liquid;
4) step 3) gained medicinal residues are added 10 times of water gagings, straight fire boils, and uses slow fire boiling 10min after boiling instead, leaches medicinal liquid;
5) step 3) and step 4) gained medicinal liquid are merged the extracting juice that removes slag.
6) the Radix Notoginseng decoction made from powder granule of paper bag packing can be by step 3), 4) and 5) method directly whole decoct a drug wrapped boil after, take out paper bag and get final product with juice.
Compare with traditional decoction pieces effective component extraction rate
Precision takes respectively organizes each about 4g of Radix Notoginseng decoction made from powder granule and traditional decoction pieces, puts in centrifuge tube, and precision adds 40ml water, weighed weight is soaked 30min, and 100 ℃ of water-baths were placed 1 hour, jolting in every 5 minutes once, taking-up lets cool, and weighed weight is again supplied the weight of less loss with water, shake up, the centrifugal 10min of 15000rpm inclines and gets supernatant, and is standby.
Precision measures above-mentioned decocting liquid 1ml, adds methanol and is diluted to 5ml, and 0.45 μ m filtering with microporous membrane is got subsequent filtrate, by " under 2010 editions First Radix Notoginseng items of Chinese pharmacopoeia, content assaying method is measured the ginsenoside Rg
1(C
42H
72O
14), ginsenoside Rb
1(C
54H
92O
23) and Panax Notoginseng saponin R
1(C
47H
80O
18) total amount, calculate total extraction ratio of these three kinds of saponin, the results are shown in Table 1-1.
Precision measures filtrate 20ml, puts in the evaporating dish that is dried to constant weight, after evaporate to dryness in water-bath, in 105 ℃ of dryings 3 hours, puts in exsiccator cooling 30 minutes, and rapid accurately weighed weight is calculated paste-forming rate (%), the results are shown in Table 1-1.
Table 1-1 Radix Notoginseng decoction made from powder granule and decoction pieces extraction ratio are relatively
| Group | Paste-forming rate (%) | Three kinds of total extraction ratios of saponin (%) |
| Radix Notoginseng decoction made from powder granule 10 orders | 37.2 | 4.82 |
| Radix Notoginseng decoction made from powder granule 20 orders | 39.4 | 5.08 |
| Radix Notoginseng decoction made from powder granule 50 orders | 40.0 | 5.11 |
| Radix Notoginseng decoction made from powder granule 100 orders | 37.8 | 5.12 |
| Common decoction pieces | 21.42 | 3.13 |
According to table 1-1 as seen, Radix Notoginseng decoction made from powder granule 20 orders and 50 purpose paste-forming rates and effective component extraction rate are optimum, and both difference is little.Consider that the fried medicine juice of 50 purpose Radix Notoginseng decoction made from powder granules is comparatively muddy, therefore select Radix Notoginseng decoction made from powder granule 20 orders further to investigate.
Mice is divided into 6 groups at random: blank group, model group, notoginseng decoction piece (1g/kg BW), Radix Notoginseng decoction made from powder granule high dose (1g/kg BW), dosage in Radix Notoginseng decoction made from powder granule (0.5g/kg BW), Radix Notoginseng decoction made from powder granule low dosage (0.3g/kg BW).Gastric infusion after each administration group medical material decocts according to above-mentioned decocting method.Except the blank group, other each dosage groups give warfarin 0.2ml/10g every day, every day 1 time, 4d altogether.Each administration group of modeling 4d begins to give relative medicine, every day 1 time.The water inlet of normally taking food of blank group and model group.Measured a bleeding time, bleeding time of the 7th day location survey in 5 days for continuously reagent.Bleeding time: measure the same day, each dosage group mice administration cut off the tail point apart from tail end 1.5cm place respectively after one hour, and was dipped in 37 ℃ of normal saline, the degree of depth is 2cm approximately, begin timing from cutting in tail, stop timing when the blood streak disappears, this section is the mice bleeding time.
T check and variance analysis are adopted in result SPSS statistical disposition.See Table 1-2.
The impact of table 1-2 on the blood coagulation disorders mice bleeding time
| Group | N | Dosage (g/kg) | The 5d bleeding time (s) | The 7d bleeding time (s) |
| The blank group | 10 | —— | 110.18±41.15 | 165.75±53.97 |
| Model group | 10 | —— | 150.34±43.23 | 201.64±63.21 |
| Notoginseng decoction piece | 10 | 1 | 103.74±46.76* | 135.95±50.01* |
| The Radix Notoginseng decoction made from powder is high | 10 | 1 | 81.43±18.10*** | 124.53±28.58** |
| In the Radix Notoginseng decoction made from powder | 10 | 0.5 | 92.47±21.55** | 131.23±34.43* |
| The Radix Notoginseng decoction made from powder is low | 10 | 0.3 | 104.64±47.05* | 140.05±70.63 |
Annotate: compare * P＜0.05**P＜0.01***P＜0.001 with model group
By table 1-2 as seen, it is substantially close to the drug effect in blood coagulation disorders mice bleeding time that granularity is 20 purpose Radix Notoginseng decoction made from powder granule low dosages and notoginseng decoction piece group, and middle dosage group and high dose group obviously are better than the notoginseng decoction piece group.Confirm, the Radix Notoginseng decoction made from powder granule of 1/3 dosage can be obtained the effect close with decoction pieces, and the Radix Notoginseng decoction made from powder granule of 1/2 dosage is better than the decoction pieces drug effect.
Embodiment 2
The method of preparation and use of Radix Ginseng preparata's scattered seed
1) get Chinese medicine Radix Ginseng decoction pieces, cross respectively 10,20,50,100 mesh sieves after pulverizing, get 4 kinds of Radix Ginseng preparata's scattered seeds;
2) get step 1) described Radix Ginseng preparata's scattered seed, be packaged into respectively 1g, 2g, 3g, 5g, 6g, 10g with paper bag or plastic bag, namely get prepared slices of Chinese crude drugs decoction made from powder formula fractional pack powder particles.
Getting when 3) using needs the Radix Ginseng preparata of dosage scattered seed, opens plastic bag and pours container into, adds 12 times of amount cold water soak 20min, and straight fire boils, and boils rear slow fire boiling 10min, leaches medicinal liquid;
4) step 3) gained medicinal residues are added 10 times of water gagings, straight fire boils, and uses slow fire boiling 10min after boiling instead, leaches medicinal liquid;
5) step 3) and step 4) gained medicinal liquid are merged the extracting juice that removes slag.
6) Radix Ginseng preparata's scattered seed of paper bag packing can be by step 3), 4) and 5) method directly whole decoct a drug wrapped boil after, take out paper bag and get final product with juice.Compare with traditional decoction pieces extraction ratio
Precision takes each about 4g of various Radix Ginseng preparata's scattered seeds and traditional decoction pieces, puts in centrifuge tube, and precision adds 40ml water, weighed weight is soaked 30min, and 100 ℃ of water-baths were placed 1 hour, jolting in every 5 minutes once, taking-up lets cool, and weighed weight is again supplied the weight of less loss with water, shake up, the centrifugal 10min of 15000rpm inclines and gets supernatant, and is standby.
Get supernatant appropriate, with 0.45 μ m filtering with microporous membrane, get subsequent filtrate, by " under 2010 editions First Radix Ginseng items of Chinese pharmacopoeia, content assaying method is measured the ginsenoside Rg
1(C
42H
72O
14), ginsenoside Re (C
48H
82O
18) and ginsenoside R
b1(C
54H
92O
23) content, calculate the extraction ratio of three kinds of saponin, the results are shown in Table 2-1.
Precision measures filtrate 20ml, puts in the evaporating dish that is dried to constant weight, after evaporate to dryness in water-bath, in 105 ℃ of dryings 3 hours, puts in exsiccator cooling 30 minutes, and rapid accurately weighed weight is calculated paste-forming rate (%), the results are shown in Table 2-1.
Table 2-1 Radix Ginseng preparata's scattered seed and decoction pieces extraction ratio are relatively
According to table 2-1 as seen, Radix Ginseng preparata's scattered seed 20 orders and 50 purpose paste-forming rates and effective component extraction rate be optimum, and zero differences substantially both.Therefore selecting Radix Ginseng preparata's scattered seed 20 orders further investigates.
Mice is divided into 5 groups at random: matched group, Radix Ginseng decoction pieces (1g/kg BW), Radix Ginseng preparata's scattered seed high dose (1g/kg BW), dosage in Radix Ginseng preparata's scattered seed (0.5g/kg BW), Radix Ginseng preparata's scattered seed low dosage (0.3g/kg BW).Gastric infusion after each administration group medical material decocts according to above-mentioned decocting method.Administration 4w, matched group give equivalent distilled water gavage.After last administration 30min, the sheet lead of mouse tail root load 5% body weight is put into the swimming trunk of 25 ℃ of water temperatures, depth of water 35cm.Begin to exhaust Post-dead duration to power from swimming with the stopwatch record, be the swimming time of mice.
Table 2-2 is on the impact of mice swimming time (X ± S)
| Group | N | Dosage (g/kg) | Swimming time (s) |
| Matched group | 10 | —— | 317±163.7 |
| The Radix Ginseng decoction pieces | 10 | 1 | 460±129.2* |
| The Radix Ginseng decoction made from powder is high | 10 | 1 | 640±101.1* |
| In the Radix Ginseng decoction made from powder | 10 | 0.5 | 527±143.0* |
| The Radix Ginseng decoction made from powder is low | 10 | 0.3 | 455±133.7* |
Annotate: compare * P＜0.05 with matched group
Each administration group sees Table 2-2 to the impact of mice swimming time (S).Result shows, each administration group all can extend the mice swimming time in various degree, and 20 order Radix Ginseng decoction made from powder low dose group are close with Radix Ginseng decoction pieces group drug effect.
Embodiment 3
The method of preparation and use of Radix Glycyrrhizae decoction made from powder granule
1) get the glycyrrhiza uralensis fisch decoction pieces, cross respectively 10,20,50,100 mesh sieves after pulverizing, get Radix Glycyrrhizae decoction made from powder granule;
2) get step 1) described Radix Glycyrrhizae decoction made from powder granule, be packaged into respectively 1g, 2g, 3g, 5g, 6g, 10g with paper bag or plastic bag, namely get prepared slices of Chinese crude drugs decoction made from powder formula fractional pack powder particles.
Getting when 3) using needs the Radix Glycyrrhizae of dosage decoction made from powder granule, opens plastic bag and pours container into, adds 12 times of amount cold water soak 20min, and straight fire boils, and boils rear slow fire boiling 10min, leaches medicinal liquid;
4) step 3) gained medicinal residues are added 10 times of water gagings, straight fire boils, and uses slow fire boiling 10min after boiling instead, leaches medicinal liquid;
5) step 3) and step 4) gained medicinal liquid are merged the extracting juice that removes slag.
6) the Radix Glycyrrhizae decoction made from powder granule of paper bag packing can be by step 3), 4) and 5) method directly whole decoct a drug wrapped boil after, take out paper bag and get final product with juice.Compare with traditional decoction pieces extraction ratio
Precision takes each about 4g of 4 kinds of Radix Glycyrrhizae decoction made from powder granules and traditional decoction pieces, puts in centrifuge tube, and precision adds 40ml water, weighed weight is soaked 30min, and 100 ℃ of water-baths were placed 1 hour, jolting in every 5 minutes once, taking-up lets cool, and weighed weight is again supplied the weight of less loss with water, shake up, the centrifugal 10min of 15000rpm inclines and gets supernatant, and is standby.
Precision measures above-mentioned decocting liquid 1ml, adds the 70%v/v alcoholic solution and is diluted to 50ml, and 0.45 μ m filtering with microporous membrane is got subsequent filtrate, by " under 2010 editions First Radix Glycyrrhizae items of Chinese pharmacopoeia, content assaying method is measured liquirtin (C
21H
22O
9) and glycyrrhizic acid (C
42H
62O
16), and calculate respectively its extraction ratio, the results are shown in Table 3-1.
Precision measures filtrate 20ml, puts in the evaporating dish that is dried to constant weight, after evaporate to dryness in water-bath, in 105 ℃ of dryings 3 hours, puts in exsiccator cooling 30 minutes, and rapid accurately weighed weight is calculated paste-forming rate (%), the results are shown in Table 3-1.
Table 3-1 Radix Glycyrrhizae decoction made from powder granule and decoction pieces extraction ratio are relatively
| Group | Paste-forming rate (%) | Liquirtin extraction ratio (%) | Glycyrrhizic acid extraction ratio (%) |
| Radix Glycyrrhizae decoction made from powder granule 10 orders | 32.23 | 0.52 | 2.06 |
| Radix Glycyrrhizae decoction made from powder granule 20 orders | 35.73 | 0.58 | 2.55 |
| Radix Glycyrrhizae decoction made from powder granule 50 orders | 35.91 | 0.59 | 2.59 |
| Radix Glycyrrhizae decoction made from powder granule 100 orders | 37.03 | 0.59 | 2.63 |
| Common decoction pieces | 20.62 | 0.38 | 1.31 |
3-1 can get according to table, Radix Glycyrrhizae decoction made from powder granule 20 orders, 50 orders, 100 purpose paste-forming rates and the basic zero difference of effective component extraction rate.Consider the facility of separating that decocts rear medicine juice and medicinal residues, therefore select Radix Glycyrrhizae decoction made from powder granule 20 orders further to investigate.
Mice is divided into 5 groups at random: matched group, licorice piece (1g/kg BW), Radix Glycyrrhizae decoction made from powder granule high dose (1g/kg BW), dosage in Radix Glycyrrhizae decoction made from powder granule (0.5g/kg BW), Radix Glycyrrhizae decoction made from powder granule low dosage (0.3g/kg BW).Gastric infusion after each administration group medical material decocts according to above-mentioned decocting method.Administration 2w, 1h after the last administration, 500ml beaker back-off inject cotton balls with syringe with 28% strong aqua ammonia 2ml in desktop, are placed in the beaker of back-off, take out cotton balls after 30s and put into mice, record cough number of times in incubation period and 3min.
The impact of table 3-2 on mouse cough incubation period and cough number of times
| Group | N | Dosage (g/kg) | Cough latent period (s) | The cough number of times |
| Matched group | 10 | —— | 37.5±9.2 | 37.8±8.6 |
| Licorice piece | 9 | 1 | 46.5±7.7* | 32.9±7.1* |
| The Radix Glycyrrhizae decoction made from powder is high | 10 | 1 | 53.7±11.1** | 25.3±4.6** |
| In the Radix Glycyrrhizae decoction made from powder | 10 | 0.5 | 48.1±6.3* | 29.4±7.2* |
| The Radix Glycyrrhizae decoction made from powder is low | 10 | 0.3 | 45.2±5.8* | 32.0±6.5* |
Annotate: compare * P＜0.05**P＜0.01 with matched group
Each administration group sees Table 3-2 to the impact of mouse cough.Result shows, each administration group all can extend mouse cough incubation period in various degree, reduces the cough number of times, and 20 order Radix Glycyrrhizae decoction made from powder low dose group are close with decoction pieces group drug effect.
Embodiment 4
The method of preparation and use of Radix Puerariae decoction made from powder granule
1) get the Chinese medicament kudzu-vine root decoction pieces, cross respectively 10,20,50,100 mesh sieves after pulverizing, get Radix Puerariae decoction made from powder granule;
2) get step 1) described 4 kinds of Radix Puerariae decoction made from powder granules, be packaged into respectively 1g, 2g, 3g, 5g, 6g, 10g with paper bag or plastic bag, namely get prepared slices of Chinese crude drugs decoction made from powder formula fractional pack powder particles.
Getting when 3) using needs the Radix Puerariae of dosage decoction made from powder granule, opens plastic bag and pours container into, adds 12 times of amount cold water soak 20min, and straight fire boils, and boils rear slow fire boiling 10min, leaches medicinal liquid;
4) step 3) gained medicinal residues are added 10 times of water gagings, straight fire boils, and uses slow fire boiling 10min after boiling instead, leaches medicinal liquid;
5) step 3) and step 4) gained medicinal liquid are merged the extracting juice that removes slag.
6) the Radix Puerariae decoction made from powder granule of paper bag packing can be by step 3), 4) and 5) method directly whole decoct a drug wrapped boil after, take out paper bag and get final product with juice.Compare with traditional decoction pieces extraction ratio
Precision takes each about 4g of 4 kinds of Radix Puerariae decoction made from powder granules and traditional decoction pieces, puts in centrifuge tube, and precision adds 40ml water, weighed weight is soaked 30min, and 100 ℃ of water-baths were placed 1 hour, jolting in every 5 minutes once, taking-up lets cool, and weighed weight is again supplied the weight of less loss with water, shake up, the centrifugal 10min of 15000rpm inclines and gets supernatant, and is standby.
Precision measures above-mentioned decocting liquid 1ml, adds the 30%v/v alcoholic solution and is diluted to 50ml, and 0.45 μ m filtering with microporous membrane is got subsequent filtrate, by " under 2010 editions First Radix Puerariae items of Chinese pharmacopoeia, content assaying method is measured puerarin (C
21H
20O
9) content, and calculate its extraction ratio, the results are shown in Table 4-1.
Precision measures filtrate 20ml, puts in the evaporating dish that is dried to constant weight, after evaporate to dryness in water-bath, in 105 ℃ of dryings 3 hours, puts in exsiccator cooling 30 minutes, and rapid accurately weighed weight is calculated paste-forming rate (%), the results are shown in Table 4-1.
Table 4-1 Radix Puerariae decoction made from powder granule and decoction pieces extraction ratio are relatively
| Group | Paste-forming rate (%) | Puerarin extraction ratio (%) |
| Radix Puerariae decoction made from powder granule 10 orders | 36.37 | 2.18 |
| Radix Puerariae decoction made from powder granule 20 orders | 40.45 | 2.52 |
| Radix Puerariae decoction made from powder granule 50 orders | 41.37 | 2.53 |
| Radix Puerariae decoction made from powder granule 100 orders | 42.86 | 2.59 |
| Common decoction pieces | 26.42 | 2.03 |
According to table 4-1 as seen, Radix Puerariae decoction made from powder granule 20 orders, 50 purpose paste-forming rates and the basic zero difference of effective component extraction rate.Consider the facility of separating that decocts rear medicine juice and medicinal residues, therefore select Radix Puerariae decoction made from powder granule 20 orders further to investigate.
The SD rat is divided into 6 groups at random: matched group, model group, Radix Puerariae decoction pieces (1g/kg BW), Radix Puerariae decoction made from powder granule high dose (1g/kg BW), dosage in Radix Puerariae decoction made from powder granule (0.5g/kg BW), Radix Puerariae decoction made from powder granule low dosage (0.3g/kg BW).After each organized gastric infusion 1w, after last administration 1h, 20% urethane (20mg/kg) intraperitoneal injection of anesthesia, opened the abdominal cavity, is the narrow left renal artery of 0.25mm silver brain clip with internal diameter, sew up, and after continuing administration every day 3w, measuring blood pressure and heart rate.
The impact of table 4-2 on renal hypertensive rat blood pressure and heart rate
| Group | N | Dosage (g/kg) | Blood pressure (mmHg) | Heart rate (inferior/minute) |
| The blank group | 10 | —— | 102.5±19.53 | 328.53±19.32 |
| Model group | 10 | —— | 146.9±24.64 | 368.47±21.63 |
| The Radix Puerariae decoction pieces | 10 | 1 | 122.1±14.60* | 342.04±18.24* |
| The Radix Puerariae decoction made from powder is high | 10 | 1 | 112.3±17.04** | 340.45±19.95* |
| In the Radix Puerariae decoction made from powder | 10 | 0.5 | 120.6±18.28* | 341.15±17.00* |
| The Radix Puerariae decoction made from powder is low | 10 | 0.3 | 124.7±17.35* | 343.77±15.22* |
Annotate: compare * P＜0.05**P＜0.01 with model group
Each administration group sees Table 4-2 to the impact of rat blood pressure heart rate.Result shows, each administration group all can reduce kidney type rat blood pressure and heart rate in various degree, and 20 order Radix Puerariae decoction made from powder low dose group are close with decoction pieces group drug effect, and middle dosage group slightly is better than common decoction pieces.
Embodiment 5
The method of preparation and use of Radix Salviae Miltiorrhizae decoction made from powder granule
1) get the salviamiltiorrhizabung decoction pieces, cross respectively 10,20,50,100 mesh sieves after pulverizing, get Radix Salviae Miltiorrhizae decoction made from powder granule;
2) get step 1) described 4 kinds of Radix Salviae Miltiorrhizae decoction made from powder granules, be packaged into respectively 1g, 2g, 3g, 5g, 6g, 10g with paper bag or plastic bag, namely get prepared slices of Chinese crude drugs decoction made from powder formula fractional pack powder particles.
Getting when 3) using needs the Radix Salviae Miltiorrhizae of dosage decoction made from powder granule, opens plastic bag and pours container into, adds 12 times of amount cold water soak 20min, and straight fire boils, and boils rear slow fire boiling 10min, leaches medicinal liquid;
4) step 3) gained medicinal residues are added 10 times of water gagings, straight fire boils, and uses slow fire boiling 10min after boiling instead, leaches medicinal liquid;
5) step 3) and step 4) gained medicinal liquid are merged the extracting juice that removes slag.
6) the Radix Salviae Miltiorrhizae decoction made from powder granule of paper bag packing can be by step 3), 4) and 5) method directly whole decoct a drug wrapped boil after, take out paper bag and get final product with juice.Compare with traditional decoction pieces extraction ratio
Precision takes each about 4g of the Radix Salviae Miltiorrhizae decoction made from powder granule of 4 kinds of granularities and traditional decoction pieces, puts in centrifuge tube, and precision adds 40ml water, weighed weight is soaked 30min, and 100 ℃ of water-baths were placed 1 hour, jolting in every 5 minutes once, taking-up lets cool, and weighed weight is again supplied the weight of less loss with water, shake up, the centrifugal 10min of 15000rpm inclines and gets supernatant, and is standby.
Precision measures above-mentioned decocting liquid 1ml, adds the 75%v/v methanol solution and is diluted to 25ml, and 0.45 μ m filtering with microporous membrane is got subsequent filtrate, by " under 2010 editions First Radix Salviae Miltiorrhizae items of Chinese pharmacopoeia, the content of danshinolic acid B assay method is measured salvianolic acid B (C
36H
30O
16) content, and calculate its extraction ratio, the results are shown in Table 5-1.
Precision measures filtrate 20ml, puts in the evaporating dish that is dried to constant weight, after evaporate to dryness in water-bath, in 105 ℃ of dryings 3 hours, puts in exsiccator cooling 30 minutes, and rapid accurately weighed weight is calculated paste-forming rate (%), the results are shown in Table 5-1.
Table 5-1 Radix Salviae Miltiorrhizae decoction made from powder granule and decoction pieces extraction ratio are relatively
| Group | Paste-forming rate (%) | Salvianolic acid B extraction ratio (%) |
| Radix Salviae Miltiorrhizae decoction made from powder granule 10 orders | 25.85 | 2.12 |
| Radix Salviae Miltiorrhizae decoction made from powder granule 20 orders | 31.04 | 2.69 |
| Radix Salviae Miltiorrhizae decoction made from powder granule 50 orders | 31.41 | 2.72 |
| Radix Salviae Miltiorrhizae decoction made from powder granule 100 orders | 34.36 | 2.79 |
| Common decoction pieces | 22.42 | 1.96 |
According to table 5-1 as seen, Radix Salviae Miltiorrhizae decoction made from powder granule 20 orders, 50 purpose paste-forming rates and the basic zero difference of effective component extraction rate.Consider the facility of separating that decocts rear medicine juice and medicinal residues, therefore select Radix Salviae Miltiorrhizae decoction made from powder granule 20 orders further to investigate.
Mice is divided into 6 groups at random: blank group, model group, salvia piece (1g/kg BW), Radix Salviae Miltiorrhizae decoction made from powder granule high dose (1g/kg BW), dosage in Radix Salviae Miltiorrhizae decoction made from powder granule (0.5g/kg BW), Radix Salviae Miltiorrhizae decoction made from powder granule low dosage (0.3g/kg BW).Gastric infusion after each administration group medical material decocts according to above-mentioned decocting method.Modeling and experimental technique are with embodiment 1.
The impact of table 5-2 on the blood coagulation disorders mice bleeding time
| Group | N | Dosage (g/kg) | The 5d bleeding time (s) | The 7d bleeding time (s) |
| The blank group | 10 | —— | 110.18±41.15 | 165.75±53.97 |
| Model group | 10 | —— | 150.34±43.23 | 201.64±63.21 |
| Salvia piece | 10 | 1 | 107.23±32.17* | 131.37±48.21* |
| The Radix Salviae Miltiorrhizae decoction made from powder is high | 10 | 1 | 77.36±25.71*** | 122.58±39.28** |
| In the Radix Salviae Miltiorrhizae decoction made from powder | 10 | 0.5 | 88.19±20.72** | 127.39±52.95** |
| The Radix Salviae Miltiorrhizae decoction made from powder is low | 10 | 0.3 | 105.21±32.08* | 134.21±68.37* |
Annotate: compare * P＜0.05**P＜0.01***P＜0.001 with model group
By table 5-2 as seen, 20 purpose Radix Salviae Miltiorrhizae decoction made from powder granule low dosages and salvia piece group are substantially close to the drug effect in blood coagulation disorders mice bleeding time, and middle dosage group and high dose group obviously are better than the salvia piece group.The Radix Salviae Miltiorrhizae decoction made from powder granule that confirms 1/3rd dosage can be obtained the effect close with decoction pieces, and the Radix Salviae Miltiorrhizae decoction made from powder granule of 1/2nd dosage is better than the decoction pieces drug effect.
Embodiment 6
The method of preparation and use of Rhizoma Coptidis decoction made from powder granule
1) get the Chinese Drug Rhizomes of Coptis decoction pieces, cross respectively 10,20,50,100 mesh sieves after pulverizing, get 4 kinds of Rhizoma Coptidis decoction made from powder granules;
2) get step 1) described 4 kinds of Rhizoma Coptidis decoction made from powder granules, be packaged into respectively 1g, 2g, 3g, 5g, 6g, 10g with paper bag or plastic bag, namely get prepared slices of Chinese crude drugs decoction made from powder formula fractional pack powder particles.
Getting when 3) using needs the Rhizoma Coptidis of dosage decoction made from powder granule, opens plastic bag and pours container into, adds 12 times of amount cold water soak 20min, and straight fire boils, and boils rear slow fire boiling 10min, leaches medicinal liquid;
4) step 3) gained medicinal residues are added 10 times of water gagings, straight fire boils, and uses slow fire boiling 10min after boiling instead, leaches medicinal liquid;
5) step 3) and step 4) gained medicinal liquid are merged the extracting juice that removes slag.
6) the Rhizoma Coptidis decoction made from powder granule of paper bag packing can be by step 3), 4) and 5) method directly whole decoct a drug wrapped boil after, take out paper bag and get final product with juice.
Compare with traditional decoction pieces extraction ratio
Precision takes each about 4g of 4 kinds of Rhizoma Coptidis decoction made from powder granules and traditional decoction pieces, puts in centrifuge tube, and precision adds 40ml water, weighed weight is soaked 30min, and 100 ℃ of water-baths were placed 1 hour, jolting in every 5 minutes once, taking-up lets cool, and weighed weight is again supplied the weight of less loss with water, shake up, the centrifugal 10min of 15000rpm inclines and gets supernatant, and is standby.
Precision measures above-mentioned decocting liquid 1ml, adds methanol and is diluted to 100ml, and 0.45 μ m filtering with microporous membrane is got subsequent filtrate, by " assay content of berberine under 2010 editions First Rhizoma Coptidis items of Chinese pharmacopoeia calculates its extraction ratio, the results are shown in Table 6-1.
Precision measures filtrate 20ml, puts in the evaporating dish that is dried to constant weight, after evaporate to dryness in water-bath, in 105 ℃ of dryings 3 hours, puts in exsiccator cooling 30 minutes, and rapid accurately weighed weight is calculated paste-forming rate (%), the results are shown in Table 6-1.
Table 6-1 Rhizoma Coptidis decoction made from powder granule and decoction pieces extraction ratio are relatively
| Group | Paste-forming rate (%) | Berberine extraction ratio (%) |
| Rhizoma Coptidis decoction made from powder granule 10 orders | 20.94 | 4.41 |
| Rhizoma Coptidis decoction made from powder granule 20 orders | 24.47 | 5.48 |
| Rhizoma Coptidis decoction made from powder granule 50 orders | 25.29 | 5.63 |
| Rhizoma Coptidis decoction made from powder granule 100 orders | 26.37 | 5.80 |
| Common decoction pieces | 19.22 | 4.29 |
According to table 6-1 as seen, Rhizoma Coptidis decoction made from powder granule 20 orders, 50 purpose paste-forming rates and the basic zero difference of effective component extraction rate.Consider the facility of separating that decocts rear medicine juice and medicinal residues, therefore select Rhizoma Coptidis decoction made from powder granule 20 orders further to investigate.
With 20 icteric sclera connect the decoction made from powder granule dilute respectively 1/2 and 1/3 as in low dose group.Get check bacterium staphylococcus aureus, dysentery bacterium, be inoculated in the ordinary broth agar slant, cultivate 18h for 37 ℃, wash lawn with the 10ml broth bouillon, be diluted to the even suspension of 0.1 times.By " 95 editions two appendix methods of Chinese pharmacopoeia are got two dish, add in stainless steel tube and respectively organize medicine 10ml, cultivate 18h for 37 ℃, measure antibacterial circle diameter.
The impact of table 6-2 on antibacterial circle diameter
| Sample | Gold Portugal's antibacterial circle diameter (mm) | Dysentery antibacterial circle diameter (mm) |
| The Rhizoma Coptidis decoction pieces | 19.7 | 21.7 |
| The Rhizoma Coptidis decoction made from powder is high | 25.2 | 28.5 |
| In the Rhizoma Coptidis decoction made from powder | 21.7 | 23.6 |
| The Rhizoma Coptidis decoction made from powder is low | 19.0 | 21.3 |
By table 6-2 as seen, it is close with common decoction pieces bacteriostasis that 20 icteric sclera connect decoction made from powder granule low dose group, and middle dosage is better than common decoction pieces.
Embodiment 7
The method of preparation and use of Ramulus Cinnamomi decoction made from powder granule
1) get prepared slices of Chinese crude drugs Ramulus Cinnamomi sheet, cross respectively 10,20,50,100 mesh sieves after pulverizing, get Ramulus Cinnamomi sheet decoction made from powder granule;
2) get step 1) described Ramulus Cinnamomi sheet decoction made from powder granule, be packaged into respectively 500g, 1kg with paper bag or plastic bag;
3) get step 2) Ramulus Cinnamomi sheet decoction made from powder granule after described packing, be placed in respectively special traditional medicine disperser, by clinician's formula of writing out a prescription, mix homogeneously namely gets prepared slices of Chinese crude drugs decoction made from powder formula.
Getting when 4) using needs the Ramulus Cinnamomi of dosage decoction made from powder granule, opens plastic bag and pours container into, adds 12 times of amount cold water soak 20min, and straight fire boils, and boils rear slow fire boiling 10min, leaches medicinal liquid;
5) step 4) gained medicinal residues are added 10 times of water gagings, straight fire boils, and uses slow fire boiling 10min after boiling instead, leaches medicinal liquid;
6) step 4) and step 5) gained medicinal liquid are merged the extracting juice that removes slag.
7) the Ramulus Cinnamomi decoction made from powder granule of paper bag packing can be by step 4), 5) and 6) method directly whole decoct a drug wrapped boil after, take out paper bag and get final product with juice.Compare with traditional decoction pieces extraction ratio
Precision takes each about 4g of 4 kinds of Ramulus Cinnamomi decoction made from powder granules and traditional decoction pieces, puts in centrifuge tube, and precision adds 40ml water, weighed weight is soaked 30min, and 100 ℃ of water-baths were placed 1 hour, jolting in every 5 minutes once, taking-up lets cool, and weighed weight is again supplied the weight of less loss with water, shake up, the centrifugal 10min of 15000rpm inclines and gets supernatant, and is standby.
Precision measures above-mentioned decocting liquid 1ml, add methanol and be diluted to 50ml, precision measures diluent 1ml and adds methanol and be diluted to 25ml again, and 0.45 μ m filtering with microporous membrane is got subsequent filtrate by " under 2010 editions First Ramulus Cinnamomi items of Chinese pharmacopoeia, content assaying method is measured cinnamic aldehyde (C
9H
8O) content calculates its extraction ratio, the results are shown in Table 7-1.
Precision measures filtrate 20ml, puts in the evaporating dish that is dried to constant weight, after evaporate to dryness in water-bath, in 105 ℃ of dryings 3 hours, puts in exsiccator cooling 30 minutes, and rapid accurately weighed weight is calculated paste-forming rate (%), the results are shown in Table 7-1.
Precision takes Ramulus Cinnamomi decoction made from powder granule and each 120g of traditional decoction pieces respectively, by " 2010 editions First appendix XD determination of volatile oil Division A League Matches of French Football methods of Chinese pharmacopoeia, add in the 1000ml flask, add water 500ml and bead number, after jolting mixes, put and slowly be heated in electric jacket boil, and keep little and boiled approximately 2 hours, stopped heating reads the volatilization oil mass in accordance with the law, and volatile oil extraction ratio (%) in calculation sample, the results are shown in Table 7-1.
Table 7-1 Ramulus Cinnamomi decoction made from powder granule and traditional decoction pieces extraction ratio and volatile oil extraction ratio are relatively
| Sample | Paste-forming rate (%) | Cinnamic aldehyde extraction ratio (%) | Volatile oil extraction ratio (%) |
| Ramulus Cinnamomi decoction made from powder granule 10 orders | 18.36 | 0.63 | 0.41 |
| Ramulus Cinnamomi decoction made from powder granule 20 orders | 24.13 | 0.92 | 0.56 |
| Ramulus Cinnamomi decoction made from powder granule 50 orders | 25.28 | 0.97 | 0.59 |
| Ramulus Cinnamomi decoction made from powder granule 100 orders | 27.14 | 1.05 | 0.61 |
| Common decoction pieces | 15.42 | 0.57 | 0.38 |
According to table 7-1 as seen, Ramulus Cinnamomi decoction made from powder granule 20 orders, 50 purpose paste-forming rates and the basic zero difference of effective component extraction rate.Consider the facility of separating that decocts rear medicine juice and medicinal residues, therefore select Ramulus Cinnamomi decoction made from powder granule 20 orders further to investigate.
Mice is divided into 6 groups at random: blank group, model group, Ramulus Cinnamomi decoction pieces (1g/kg BW), Ramulus Cinnamomi decoction made from powder granule high dose (1g/kg BW), dosage in Ramulus Cinnamomi decoction made from powder granule (0.5g/kg BW), Ramulus Cinnamomi decoction made from powder granule low dosage (0.3g/kg BW).The blank group is respectively at experiment the 1st, 7,14 and 21d Intraperitoneal injection normal saline 0.2ml; 28d begins to atomize with normal saline and sucks 30min, 60min normal saline 0.5ml gavage, 7d continuously before atomizing sucks.Model group is got the normal saline 0.2ml that contains egg protein 10 μ g, aluminium hydroxide 20mg, in the 1st, 7,14 and the 21d Intraperitoneal injection; 28d begins to atomize with the normal saline that mass fraction is 5% egg protein and sucks 30min, 60min normal saline 0.5ml gavage, 7d continuously before atomizing sucks.Each administration group mouse sensitization with excite same asthma group.60min medicine gavage before atomizing sucks, 7d continuously.Test the disconnected marrow of 35d and put to death mice, dissect and take out lungs, measure IFN-γ, IL-4.
Table 7-2 asthmatic mouse IFN-γ, IL-4 are relatively
| Group | N | Dosage (g/kg) | IFN-γ | IL-4 |
| The blank group | 9 | —— | 3.4248±0.9986 | 2.1675±0.2262 |
| Model group | 10 | —— | 14.3124±2.7473 | 21.9564±7.8421 |
| The Ramulus Cinnamomi decoction pieces | 10 | 1 | 24.2326±3.1377* | 5.3587±1.2361** |
| The Ramulus Cinnamomi decoction made from powder is high | 10 | 1 | 26.3376±4.7157* | 4.1658±1.0628** |
| In the Ramulus Cinnamomi decoction made from powder | 10 | 0.5 | 25.1956±3.2672* | 4.8439±1.9645** |
| The Ramulus Cinnamomi decoction made from powder is low | 10 | 0.3 | 24.2841±3.0158* | 5.7421±1.7437** |
Annotate: compare * P＜0.05**P＜0.01 with model group
By table 7-2 as seen, 20 order Ramulus Cinnamomi decoction made from powder granule low dosages are substantially close to the mouse asthma drug effect with Ramulus Cinnamomi decoction pieces group, and middle dosage group slightly is better than Ramulus Cinnamomi decoction pieces group.The Ramulus Cinnamomi decoction made from powder granule that confirms 1/3rd dosage can be obtained the effect close with decoction pieces.
Embodiment 8
The method of preparation and use of Semen Nelumbinis decoction made from powder granule
1) get the Semen Nelumbinis decoction pieces, cross respectively 10,20,50,100 mesh sieves after pulverizing, get 4 kinds of Semen Nelumbinis decoction made from powder granules;
2) get step 1) described Semen Nelumbinis decoction made from powder granule, be packaged into respectively 500g, 1kg with paper bag or plastic bag;
3) get step 2) Semen Nelumbinis decoction made from powder granule after described packing, be placed in respectively special traditional medicine disperser, by clinician's formula of writing out a prescription, mix homogeneously namely gets prepared slices of Chinese crude drugs decoction made from powder formula.
Getting when 4) using needs the Semen Nelumbinis of dosage decoction made from powder granule, opens plastic bag and pours container into, adds 12 times of amount cold water soak 20min, and straight fire boils, and boils rear slow fire boiling 10min, leaches medicinal liquid;
5) step 4) gained medicinal residues are added 10 times of water gagings, straight fire boils, and uses slow fire boiling 10min after boiling instead, leaches medicinal liquid;
6) step 4) and step 5) gained medicinal liquid are merged the extracting juice that removes slag.
7) the Semen Nelumbinis decoction made from powder granule of paper bag packing can be by step 4), 5) and 6) method directly whole decoct a drug wrapped boil after, take out paper bag and get final product with juice.
Compare with traditional decoction pieces extraction ratio
Precision takes each about 4g of 4 kinds of Semen Nelumbinis decoction made from powder granules and traditional decoction pieces, puts in centrifuge tube, and precision adds 40ml water, weighed weight, soak 30min, 100 ℃ of water-baths were placed 1 hour, and jolting in every 5 minutes once, taking-up lets cool, weighed weight is again supplied the weight of less loss with water, shake up, the centrifugal 10min of 15000rpm inclines and gets supernatant.Precision measures filtrate 20ml, put in the evaporating dish that is dried to constant weight, after evaporate to dryness in water-bath, in 105 ℃ of dryings 3 hours, put in exsiccator cooling 30 minutes, rapid accurately weighed weight, calculating composition extraction ratio (%), the composition extraction ratio of Semen Nelumbinis decoction made from powder granule 10,20,50,100 orders and traditional decoction pieces is respectively 7.36%, 11.54%, 12.42%, 13.93% and 4.23% as a result.Consider the facility of separating that decocts rear medicine juice and medicinal residues, select Semen Nelumbinis decoction made from powder granule 20 orders further to investigate.
Mice is divided into 6 groups at random: blank group, model group, Semen Nelumbinis decoction pieces (1g/kg BW), Semen Nelumbinis decoction made from powder granule high dose (1g/kg BW), dosage in Semen Nelumbinis decoction made from powder granule (0.5g/kg BW), Semen Nelumbinis decoction made from powder granule low dosage (0.3g/kg BW).Model group and each administration group nape every day subcutaneous injection D-of section galactose (normal saline is made into 5%, and injection volume is 0.25mL/10g) are made the carbohydrate metabolism aging model, give continuously D-galactose 40d.Since the 1st day, gastric infusion and with the volume normal saline respectively, administration every day 1 time, successive administration 30d.2h after giving D-galactose and perfusion last 1 time, mouse orbit is got blood, and anticoagulant heparin is measured antioxidation.
Table 8 couple aging model Mouse Blood SOD, the impact of CAT vigor
| Group | N | Dosage (g/kg) | SOD vigor (U/L) | The CAT vigor (* 10-4) |
| The blank group | 9 | —— | 2037±703 | 2.983±0.387 |
| Model group | 10 | —— | 964±473 | 1.715±0.421 |
| The Semen Nelumbinis decoction pieces | 10 | 1 | 1435±536* | 2.937±0.361* |
| The Semen Nelumbinis decoction made from powder is high | 10 | 1 | 1689±731* | 3.558±0.706* |
| In the Semen Nelumbinis decoction made from powder | 10 | 0.5 | 1529±408* | 3.137±0.640* |
| The Semen Nelumbinis decoction made from powder is low | 10 | 0.3 | 1473±484* | 2.874±0.437* |
Annotate: compare * P＜0.01 with model group
By as seen from Table 8,20 order Semen Nelumbinis decoction made from powder granule low dosages are substantially close to the mouse aging antioxidation with Semen Nelumbinis decoction pieces group, and middle dosage group slightly is better than Semen Nelumbinis decoction pieces group.The Semen Nelumbinis decoction made from powder granule that confirms 1/3rd dosage can be obtained the effect close with common decoction pieces.
Embodiment 9
Preparation and the decoction of Fructus Corni decoction made from powder granule:
1) pulverize: Chinese medicine Fructus Corni decoction pieces is crushed to respectively 10,20,50,100 orders, gets 4 kinds of Fructus Corni decoction made from powder granules;
2) take the above-mentioned Fructus Corni decoction made from powder of 30g granule, with the non-woven bag parcel, add 8 times of amount cold water soak 20min, straight fire boils, and boils rear slow fire boiling 30min, leaches medicinal liquid;
3) with step 2) the gained medicinal residues add 6 times of water gagings, and straight fire boils, and uses slow fire boiling 20min after boiling instead, leaches medicinal liquid;
4) with step 2) and step 3) gained medicinal liquid merge, be concentrated into driedly, weigh, calculate paste volume and paste-forming rate.
The decoction of Fructus Corni decoction pieces:
1) take Fructus Corni decoction pieces 90g, with the non-woven bag parcel, add 12 times of amount cold water soak 20min, straight fire boils, and boils rear slow fire boiling 10min, leaches medicinal liquid;
2) step 1) gained medicinal residues are added 10 times of water gagings, straight fire boils, and uses slow fire boiling 10min after boiling instead, leaches medicinal liquid;
3) with step 1) and step 2) the gained medicinal liquid merges, and is concentrated into driedly, weighs, and calculates paste volume and paste-forming rate.
With reference to " Determination of Loganin in Chinese pharmacopoeia high effective liquid chromatography for measuring gained soft extracts also calculates the loganin extraction ratio.The results are shown in Table 9-1.
The comparison of table 9-1 Fructus Corni decoction made from powder granule and decoction pieces extraction ratio
| Group | Paste-forming rate (%) | Loganin extraction ratio (%) |
| Fructus Corni decoction made from powder granule 10 orders | 47.2 | 12.79 |
| Fructus Corni decoction made from powder granule 20 orders | 49.1 | 12.94 |
| Fructus Corni decoction made from powder granule 50 orders | 50.7 | 13.38 |
| Fructus Corni decoction made from powder granule 100 orders | 50.3 | 13.38 |
| The Fructus Corni decoction pieces | 21.42 | 5.73 |
According to table 9-1 as seen, Fructus Corni decoction made from powder granule 20 orders, 50 purpose paste-forming rates and the basic zero difference of effective component extraction rate.Consider the facility of separating that decocts rear medicine juice and medicinal residues, therefore select Fructus Corni decoction made from powder granule 20 orders further to investigate.
Mice is divided into 5 groups at random: matched group, Fructus Corni decoction pieces (1g/kg BW), Fructus Corni decoction made from powder granule high dose (1g/kg BW), dosage in Fructus Corni decoction made from powder granule (0.5g/kg BW), Fructus Corni decoction made from powder granule low dosage (0.3g/kg BW).Hypoxia endurance test carries out in the airtight wide mouthed bottle of 100ml, mice in every bottle, with soda lime as CO
2Indicator.
The impact of table 9-2 on the anoxia in mice toleration
| Group | N | Dosage (g/kg) | The hypoxic tolerance time (s) |
| Matched group | 9 | —— | 468.3±87.7 |
| The Fructus Corni decoction pieces | 10 | 1 | 642.9±74.1* |
| The Fructus Corni decoction made from powder is high | 10 | 1 | 735.8±115.7* |
| In the Fructus Corni decoction made from powder | 10 | 0.5 | 683.1±90.6* |
| The Fructus Corni decoction made from powder is low | 10 | 0.3 | 631.4±52.4* |
Annotate: compare * P＜0.01 with model group
By table 9-2 as seen, 20 purpose Fructus Corni decoction made from powder granule low dosages are substantially close to the hypoxia mice resisting oxygen lack with Fructus Corni decoction pieces group.The Fructus Corni decoction made from powder granule that confirms 1/3rd dosage can be obtained the effect close with common decoction pieces.
Embodiment 10
Preparation and the decoction of Herba Hedyotidis Diffusae decoction made from powder granule:
1) pulverize: cross respectively 10 orders, 20 orders, 50 orders and 80 mesh sieves after the Herba Hedyotidis Diffusae decoction pieces is pulverized, get Herba Hedyotidis Diffusae decoction made from powder granule;
2) take above-mentioned three kinds of each 90g of Herba Hedyotidis Diffusae decoction made from powder granule, respectively with the non-woven bag parcel, add 12 times of amount cold water soak 20min, straight fire boils, and boils rear slow fire boiling 10min, leaches medicinal liquid;
3) with step 2) the gained medicinal residues add respectively 10 times of water gagings, and straight fire boils, and uses slow fire boiling 10min after boiling instead, leaches medicinal liquid;
4) with step 2) and step 3) gained medicinal liquid merge, be concentrated into driedly, weigh, calculate paste volume and paste-forming rate.
The decoction of Herba Hedyotidis Diffusae decoction pieces:
1) get and need the Herba Hedyotidis Diffusae of dosage decoction pieces granule, put into container with the non-woven bag parcel, add 10 times of amount cold water soak 20min, straight fire boils, and boils rear slow fire boiling 10min, leaches medicinal liquid;
2) step 1) gained medicinal residues are added 8 times of water gagings, straight fire boils, and uses slow fire boiling 10min after boiling instead, leaches medicinal liquid;
3) with step 1) and step 2) the gained medicinal liquid merges the extracting juice that removes slag.Be concentrated into driedly, weigh, calculate paste-forming rate.
Content of oleanolic acid in tlc scanning determination gained soft extracts also calculates the oleanolic acid extraction ratio.The results are shown in Table 10.
The comparison of table 10 Herba Hedyotidis Diffusae decoction made from powder granule and decoction pieces paste-forming rate and extraction ratio
Paste-forming rate and oleanolic acid extraction ratio than its decoction of Herba Hedyotidis Diffusae decoction pieces when as shown in Table 10, Chinese medicine Herba Hedyotidis Diffusae decoction made from powder grain diameter of the present invention is 10 ~ 50 order all obviously increase.Wherein Herba Hedyotidis Diffusae decocts scattered seed and selects 20 orders, paste-forming rate and oleanolic acid extraction ratio all more common decoction pieces and 10 orders to decoct scattered seed high, therefore and convenient operation again, is selected Herba Hedyotidis Diffusae to decoct loose particle diameter and is preferably 20 orders.
Embodiment 11
The method of preparation and use of Flos Lonicerae decoction made from powder granule
1) extracting honeysuckle decoction pieces is crossed respectively 10,20,50,80 mesh sieves after pulverizing, get Flos Lonicerae decoction made from powder granule;
2) get step 1) described Flos Lonicerae decoction made from powder granule, be packaged into respectively 500g, 1kg with paper bag or plastic bag;
3) get step 2) Flos Lonicerae decoction made from powder granule after described packing, be placed in respectively special traditional medicine disperser, by clinician's formula of writing out a prescription, mix homogeneously namely gets prepared slices of Chinese crude drugs decoction made from powder formula.
Getting when 4) using needs the Flos Lonicerae of dosage decoction made from powder granule, opens plastic bag and pours container into, adds 10 times of amount cold water soak 20min, and straight fire boils, and boils rear slow fire boiling 10min, leaches medicinal liquid;
5) step 4) gained medicinal residues are added 10 times of water gagings, straight fire boils, and uses slow fire boiling 10min after boiling instead, leaches medicinal liquid;
6) step 4) and step 5) gained medicinal liquid are merged the extracting juice that removes slag.
7) the Flos Lonicerae decoction made from powder granule of paper bag packing can be by step 4), 5) and 6) method directly whole decoct a drug wrapped boil after, take out paper bag and get final product with juice.
Compare with traditional decoction pieces extraction ratio
Precision takes each about 4g of 4 kinds of Flos Lonicerae decoction made from powder granules and traditional decoction pieces, puts in centrifuge tube, and precision adds 40ml water, weighed weight is soaked 30min, and 100 ℃ of water-baths were placed 1 hour, jolting in every 5 minutes once, taking-up lets cool, and weighed weight is again supplied the weight of less loss with water, shake up, the centrifugal 10min of 15000rpm inclines and gets supernatant, and is standby.
Precision measures above-mentioned decocting liquid 1ml, adds methanol and is diluted to 50ml, and 0.45 μ m filtering with microporous membrane is got subsequent filtrate by " under 2010 editions First Flos Lonicerae items of Chinese pharmacopoeia, the determination of chlorogenic acid method is measured chlorogenic acid (C
16H
18O
9) content, calculate chlorogenic acid yield; Separately directly get decocting liquid and filter with 0.45 μ m microporous filter membrane, get subsequent filtrate by " under 2010 editions First Flos Lonicerae items of Chinese pharmacopoeia, the content of luteolin assay method is measured luteolin (C
21H
20O
11) content, calculate the luteolin extraction ratio.The results are shown in Table 11-1.
Precision measures filtrate 20ml, puts in the evaporating dish that is dried to constant weight, after evaporate to dryness in water-bath, in 105 ℃ of dryings 3 hours, puts in exsiccator cooling 30 minutes, and rapid accurately weighed weight is calculated paste-forming rate (%), the results are shown in Table 11-1.
Table 11-1 Flos Lonicerae decoction made from powder granule and traditional decoction pieces extraction ratio are relatively
By table 11-1 as can be known, when Flos Lonicerae decoction made from powder grain diameter of the present invention is 10 ~ 50 order, paste-forming rate and the effective component extraction rate than its decoction of decoction pieces all obviously increases.Wherein Flos Lonicerae decocts scattered seed and selects 20 orders, yield of extract and effective component extraction rate all more common decoction pieces and 10 orders to decoct scattered seed high, therefore and convenient operation again, is selected Flos Lonicerae to decoct loose particle diameter and is preferably 20 orders and further studies.
With 20 order Flos Lonicerae decoction made from powder granules dilute respectively 1/2 and 1/3 as in low dose group.Get check bacterium staphylococcus aureus, dysentery bacterium, be inoculated in the ordinary broth agar slant, cultivate 18h for 37 ℃, wash lawn with the 10ml broth bouillon, be diluted to the even suspension of 0.1 times.By " 95 editions two appendix methods of Chinese pharmacopoeia are got two dish, add in stainless steel tube and respectively organize medicine 10ml, cultivate 18h for 37 ℃, measure antibacterial circle diameter.
The impact of table 11-2 on antibacterial circle diameter
| Sample | Gold Portugal's antibacterial circle diameter (mm) | Dysentery antibacterial circle diameter (mm) |
| The Flos Lonicerae decoction pieces | 19.8 | 21.0 |
| The Flos Lonicerae decoction made from powder is high | 25.5 | 27.8 |
| In the Flos Lonicerae decoction made from powder | 21.4 | 22.4 |
| The Flos Lonicerae decoction made from powder is low | 19.8 | 21.2 |
By table 11-2 as seen, 20 order Flos Lonicerae decoction made from powder granule 1/3 dosage groups are close with common decoction pieces bacteriostasis, and 1/2 dosage is better than common decoction pieces.
Embodiment 12
The method of preparation and use of Folium Perillae decoction made from powder granule
1) get the Folium Perillae decoction pieces, cross respectively 10,20,50 mesh sieves after pulverizing, get 3 kinds of Folium Perillae decoction made from powder granules;
2) get step 1) described Folium Perillae decoction made from powder granule, be packaged into respectively 500g, 1kg with paper bag or plastic bag;
3) get step 2) Folium Perillae decoction made from powder granule after described packing, be placed in respectively special traditional medicine disperser, by clinician's formula of writing out a prescription, mix homogeneously namely gets prepared slices of Chinese crude drugs decoction made from powder formula.
Getting when 4) using needs the Folium Perillae of dosage decoction made from powder granule, opens plastic bag and pours container into, adds 10 times of amount cold water soak 15min, and straight fire boils, and boils rear slow fire boiling 10min, leaches medicinal liquid;
5) step 4) gained medicinal residues are added 10 times of water gagings, straight fire boils, and uses slow fire boiling 5min after boiling instead, leaches medicinal liquid;
6) step 4) and step 5) gained medicinal liquid are merged the extracting juice that removes slag.
7) the Folium Perillae decoction made from powder granule of paper bag packing can be by step 4), 5) and 6) method directly whole decoct a drug wrapped boil after, take out paper bag and get final product with juice.
Compare with traditional decoction pieces volatile oil extraction ratio
Precision takes 3 kinds of Folium Perillae decoction made from powder granules and each 150g of traditional decoction pieces respectively, by " 2010 editions First appendix XD determination of volatile oil Division A League Matches of French Football methods of Chinese pharmacopoeia, add in the 1000ml flask, add water 600ml and bead number, after jolting mixes, put and slowly be heated in electric jacket boil, and keep little and boiled approximately 2.5 hours, stopped heating reads the volatilization oil mass in accordance with the law, and the extraction ratio (%) of volatile oil in calculation sample, the results are shown in Table 12.Precision measures filtrate 20ml, puts in the evaporating dish that is dried to constant weight, after evaporate to dryness in water-bath, in 105 ℃ of dryings 3 hours, puts in exsiccator cooling 30 minutes, and rapid accurately weighed weight is calculated paste-forming rate (%).
Table 12 Folium Perillae decoction made from powder granule and traditional decoction pieces paste-forming rate and volatile oil extraction ratio are relatively
| Sample | Paste-forming rate (%) | Volatile oil extraction ratio (%) |
| Folium Perillae decoction made from powder granule 10 orders | 9.95 | 0.38 |
| Folium Perillae decoction made from powder granule 20 orders | 10.63 | 0.43 |
| Folium Perillae decoction made from powder granule 50 orders | 11.13 | 0.45 |
| Common decoction pieces | 9.62 | 0.36 |
Paste-forming rate and volatile oil extraction ratio than its decoction of decoction pieces when as shown in Table 12, Folium Perillae decoction made from powder grain diameter of the present invention is 10 ~ 50 order all obviously increase.Wherein Folium Perillae decocts scattered seed and selects 20 orders, paste-forming rate and volatile oil extraction ratio all more common decoction pieces and 10 orders to decoct scattered seed high, therefore and convenient operation again, is selected Folium Perillae to decoct loose particle diameter and is preferably 20 orders.
Embodiment 13
Preparation and the decoction of Concha Ostreae decoction made from powder granule
1) get the Concha Ostreae decoction pieces, cross 60,100,200 mesh sieves after pulverizing, get 3 seed oyster decoction made from powder granules;
2) get step 1) described Concha Ostreae decoction made from powder granule, be packaged into respectively 1g, 2g, 3g, 5g, 6g, 10g with paper bag or plastic bag, namely get prepared slices of Chinese crude drugs decoction made from powder formula fractional pack powder particles.
3) take above-mentioned three each 72g of seed oyster decoction made from powder granule, open plastic bag and pour the decoction made from powder granule into the decoction container, add 12 times of amount cold water soak 60min, straight fire boils, and boils rear slow fire boiling 40min, leaches medicinal liquid;
4) step 3) gained medicinal residues are added 10 times of water gagings, straight fire boils, and uses slow fire boiling 30min after boiling instead, leaches medicinal liquid;
5) step 3) and step 4) gained medicinal liquid are merged, extracting juice removes slag.
6) the Concha Ostreae decoction made from powder granule of paper bag packing is directly put into the decoction container with paper bag, by step 3), 4) and 5) after method decocts, taking-up paper bag juice.
7) step 5) gained medicinal liquid is concentrated into dried, weighs, calculate extraction ratio, the results are shown in Table 13.
The comparison of table 13 Concha Ostreae decoction made from powder granule and decoction pieces extraction ratio
| Group | Pulverize yield (%) | Extraction ratio (%) |
| Concha Ostreae decoction made from powder 200 order granules | 80.12 | 5.95 |
| Concha Ostreae decoction made from powder 100 order granules | 95.02 | 5.87 |
| Concha Ostreae decoction made from powder 60 order granules | 94.96 | 3.55 |
| The Concha Ostreae decoction pieces | - | 2.27 |
Extraction ratio than its decoction of decoction pieces when as shown in Table 13, Concha Ostreae decoction made from powder grain diameter of the present invention is 60 ~ 200 order obviously increases.Concha Ostreae decoction made from powder 100 order granules wherein, extraction ratio obviously increases than 60 orders, compares with 200 orders that to pulverize yield higher, therefore determine that its particle diameter is preferably 100 orders.
Embodiment 14
Preparation and the decoction of Carapax Et Plastrum Testudinis decoction made from powder granule:
1) pulverize: cross respectively 60 orders, 100 orders, 200 mesh sieves after Chinese medicine Carapax Et Plastrum Testudinis decoction pieces is pulverized, get Carapax Et Plastrum Testudinis decoction made from powder granule;
2) take above-mentioned three kinds of above-mentioned each 72g of Carapax Et Plastrum Testudinis decoction made from powder granule, respectively with the non-woven bag parcel, add 12 times of amount cold water soak 60min, straight fire boils, and boils rear slow fire boiling 40min, leaches medicinal liquid;
3) with step 2) the gained medicinal residues add 10 times of water gagings, and straight fire boils, and uses slow fire boiling 30min after boiling instead, leaches medicinal liquid;
4) with step 2) and step 3) gained medicinal liquid merge, be concentrated into driedly, weigh, calculate extraction ratio, the results are shown in Table 14-1.
The decoction of Carapax Et Plastrum Testudinis decoction pieces:
1) get and need the Carapax Et Plastrum Testudinis of dosage decoction made from powder granule, directly put into container with the non-woven bag parcel, add 12 times of amount cold water soak 60min, straight fire boils, and boils rear slow fire boiling 40min, leaches medicinal liquid;
2) step 1) gained medicinal residues are added 10 times of water gagings, straight fire boils, and uses slow fire boiling 30min after boiling instead, leaches medicinal liquid;
3) with step 1) and step 2) the gained medicinal liquid merges the extracting juice that removes slag.Be concentrated into driedly, weigh, calculate extraction ratio, the results are shown in Table 14-1.The comparison of table 14-1 Carapax Et Plastrum Testudinis decoction made from powder granule and decoction pieces extraction ratio
| Group | Pulverize yield (%) | Extraction ratio (%) |
| Carapax Et Plastrum Testudinis decoction made from powder 100 order granules | 98.16 | 5.71 |
| Carapax Et Plastrum Testudinis decoction made from powder 200 order granules | 82.42 | 5.84 |
| Carapax Et Plastrum Testudinis decoction made from powder 60 order granules | 98.35 | 3.10 |
| The Carapax Et Plastrum Testudinis decoction pieces | - | 2.09 |
By table 14-1 as can be known, when Chinese medicine Carapax Et Plastrum Testudinis decoction made from powder grain diameter of the present invention is 60 ~ 200 order, the extraction ratio than its decoction of Carapax Et Plastrum Testudinis decoction pieces all obviously increases.Carapax Et Plastrum Testudinis decoction made from powder 100 order granules wherein, extraction ratio obviously increases than 60 orders, approximately increases by 1 times, and when pulverizing 200 order, the operation more complicated is pulverized yield lower, but it is suitable with 100 orders to decoct out cream, therefore definite its particle diameter is preferably 100 orders.Therefore selecting 100 order Carapax Et Plastrum Testudinis decoction made from powder granules further investigates.
Mice is divided into 6 groups at random: the blank group, model, Carapax Et Plastrum Testudinis decoction pieces (0.3g/kg BW), Carapax Et Plastrum Testudinis decoction made from powder granule high dose (0.3g/kg BW), dosage in Carapax Et Plastrum Testudinis decoction made from powder granule (0.15g/kg BW), Carapax Et Plastrum Testudinis decoction made from powder granule low dosage (0.1g/kg BW), the above decoction made from powder of respectively organizing is 100 order Carapax Et Plastrum Testudinis decoction made from powder granules.3mg thyroid tablet and 0.02mg reserpine solution/20g are to the mice modeling, and modeling in the 9th day is successful.Blank group is to 3% tween solution of equivalent, and treatment group is in the modeling while, and gavage gives each medicine, 1 time/d, is total to 8d.Every day, the mice weighing, calculated Mouse Weight recruitment after administration, and the SPSS16.0 statistical software analyzes.The results are shown in Table 14-2.
The impact of table 14-2 on deficiency of YIN Mouse Weight
| Group | N | Dosage (g/kg) | Body weight increases (g) |
| The blank group | 9 | —— | 3.13±1.78 |
| Model group | 9 | —— | -3.52±1.25 |
| The Carapax Et Plastrum Testudinis decoction pieces | 10 | 0.3 | -1.83±1.11* |
| The Carapax Et Plastrum Testudinis decoction made from powder is high | 10 | 0.3 | -1.28±1.07** |
| In the Carapax Et Plastrum Testudinis decoction made from powder | 10 | 0.15 | -1.71±1.06* |
| The Carapax Et Plastrum Testudinis decoction made from powder is low | 10 | 0.1 | -1.80±1.40* |
By table 14-2 as seen, 100 order Carapax Et Plastrum Testudinis decoction made from powder granule 1/3 dosage are close to the effect of deficiency of YIN Mouse Weight with common decoction pieces.The Carapax Et Plastrum Testudinis decoction made from powder granule effect of 1/2 dosage and same dose is better than common decoction pieces.
Embodiment 15
The preparation of Talcum decoction made from powder granule
1) get the Talcum decoction pieces, cross respectively 60,100,200 mesh sieves after pulverizing, get Talcum decoction made from powder granule;
2) get step 1) described Talcum decoction made from powder granule, be packaged into respectively 1g, 2g, 3g, 5g, 6g, 10g with paper bag or plastic bag;
3) get step 2) Talcum decoction made from powder granule after described packing, be placed in respectively special traditional medicine disperser, by clinician's formula of writing out a prescription, mix homogeneously namely gets prepared slices of Chinese crude drugs decoction made from powder formula.
The decoction of Talcum decoction made from powder granule:
1) take above-mentioned three kinds of each 72g of Talcum decoction made from powder granule, respectively with the non-woven bag parcel, add 12 times of amount cold water soak 60min, straight fire boils, and boils rear slow fire boiling 40min, leaches medicinal liquid;
2) with step 2) the gained medicinal residues add 10 times of water gagings, and straight fire boils, and uses slow fire boiling 30min after boiling instead, leaches medicinal liquid;
3) with step 1) and step 2) the gained medicinal liquid merges, and is concentrated into driedly, weighs, and calculates extraction ratio, the results are shown in Table 15-1.
The comparison of table 15-1 Talcum decoction made from powder granule and decoction pieces extraction ratio
| Group | Pulverize yield (%) | Extraction ratio (%) |
| Talcum decoction made from powder 200 order granules | 80.86 | 6.82 |
| Talcum decoction made from powder 100 order granules | 87.82 | 6.25 |
| Talcum decoction made from powder 60 order granules | 98.11 | 4.90 |
| The Talcum decoction pieces | - | 3.52 |
By table 15-1 as can be known, when Talcum decoction made from powder grain diameter of the present invention is 60 ~ 200 order, the extraction ratio than its decoction of decoction pieces all obviously increases.Wherein 100 order granule extraction ratios obviously increase than 60 orders, and time operation is cumbersome and pulverize 200 order, pulverize yield lower, but it are suitable with 100 orders to decoct out cream, therefore definite its particle diameter is preferably 100 orders.Therefore selecting 100 order Talcum decoction made from powder granules further investigates.
The SD rat is divided into 5 groups at random: matched group, Talcum decoction pieces (100mg/kg BW), Talcum decoction made from powder granule high dose (100mg/kg BW), dosage in Talcum decoction made from powder granule (50mg/kg BW), Talcum decoction made from powder granule low dosage (30mg/kg BW), the above decoction made from powder of respectively organizing is 100 order Talcum decoction made from powder granules.The FCA of 0.1ml/rat is injected the right sufficient sole of the foot of rat, after 2 weeks, confirm that arthritis is induced.Gastric infusion 2w measures joint edema situation.The results are shown in Table 15-2.
The impact of table 15-2 on rat joint edema variation
| Group | N | Dosage (mg/kg) | Swelling |
| Matched group | 9 | —— | 52.69±2.70 |
| The Talcum decoction pieces | 10 | 100 | 44.26±2.51 |
| The Talcum decoction made from powder is high | 10 | 100 | 43.28±2.07 |
| In the Talcum decoction made from powder | 10 | 50 | 43.51±2.06 |
| The Talcum decoction made from powder is low | 10 | 30 | 44.86±1.98 |
By table 15-2 as seen, all there is no statistical significance although each group is compared with matched group, Talcum is respectively organized swelling and is relatively reduced, and 1/3 dosage group is suitable with the decoction pieces effect.
Embodiment 16
The method of preparation and use of Pheretima decoction made from powder granule
1) get earthworm decocting pieces, cross respectively 10,20,50 mesh sieves after pulverizing, get Pheretima decoction made from powder granule;
2) get step 1) described Pheretima decoction made from powder granule, be packaged into respectively 1g, 2g, 3g, 5g, 6g, 10g with paper bag or plastic bag;
3) get step 2) Pheretima decoction made from powder granule after described packing, be placed in respectively special traditional medicine disperser, by clinician's formula of writing out a prescription, mix homogeneously namely gets prepared slices of Chinese crude drugs decoction made from powder formula.
Getting when 4) using needs the Pheretima of dosage decoction made from powder granule, opens plastic bag and pours container into, adds 12 times of amount cold water soak 20min, and straight fire boils, and boils rear slow fire boiling 10min, leaches medicinal liquid;
5) step 4) gained medicinal residues are added 10 times of water gagings, straight fire boils, and uses slow fire boiling 10min after boiling instead, leaches medicinal liquid;
6) step 4) and step 5) gained medicinal liquid are merged the extracting juice that removes slag.
7) the Pheretima decoction made from powder granule of paper bag packing can be by step 4), 5) and 6) method directly whole decoct a drug wrapped boil after, take out paper bag and get final product with juice.
Measure as follows the content of 3 kinds of Pheretima decoction made from powder granules and common decoction pieces water-soluble extractives.
Get approximately 3g of test sample, accurately weighed, to put in the conical flask of 250ml, precision adds water 50ml, close plug, weighed weight after standing 1 hour, connects reflux condensing tube, is heated to boiling, and keeps little and boiled 1 hour.After letting cool, take off conical flask, close plug, weighed weight again, water is supplied the weight of less loss, shakes up, filter with dry filter, precision measures filtrate 25ml, puts in the evaporating dish that is dried to constant weight, after evaporate to dryness in water-bath, in 105 ℃ of dryings 3 hours, put in exsiccator cooling 30 minutes, rapid accurately weighed weight is calculated the content (%) of water-soluble extractives in test sample with dry product.The results are shown in Table 16.
The comparison of table 16 Pheretima decoction made from powder granule and decoction pieces water-soluble extractives content
| Group | Water-soluble extractives (%) |
| Pheretima decoction made from powder granule 50 orders | 20.62 |
| Pheretima decoction made from powder granule 20 orders | 19.21 |
| Pheretima decoction made from powder granule 10 orders | 17.90 |
| Earthworm decocting pieces | 16.52 |
Obviously increase than its water-soluble extractives of decoction pieces when as shown in Table 16, Pheretima decoction made from powder grain diameter of the present invention is 10 ~ 50 order.Because the muddy difficulty of 50 order decocting liquids is divided, therefore select 20 order Pheretima decoction made from powder granules for preferred.
Embodiment 17
The method of preparation and use of Hericium erinaceus (Bull. Ex Fr.) Pers. decoction made from powder granule
1) get the Hericium erinaceus (Bull. Ex Fr.) Pers. decoction pieces, cross 20 mesh sieves after pulverizing, get Hericium erinaceus (Bull. Ex Fr.) Pers. decoction made from powder granule;
2) get step 1) described Hericium erinaceus (Bull. Ex Fr.) Pers. decoction made from powder granule, be packaged into respectively 1g, 2g, 3g, 5g, 6g, 10g with paper bag or plastic bag;
3) get step 2) Hericium erinaceus (Bull. Ex Fr.) Pers. decoction made from powder granule after described packing, be placed in respectively special traditional medicine disperser, by clinician's formula of writing out a prescription, mix homogeneously namely gets prepared slices of Chinese crude drugs decoction made from powder formula.
Getting when 4) using needs the Hericium erinaceus (Bull. Ex Fr.) Pers. of dosage decoction made from powder granule, opens plastic bag and pours container into, adds 12 times of amount cold water soak 20min, and straight fire boils, and boils rear slow fire boiling 10min, leaches medicinal liquid;
5) step 4) gained medicinal residues are added 10 times of water gagings, straight fire boils, and uses slow fire boiling 10min after boiling instead, leaches medicinal liquid;
6) step 4) and step 5) gained medicinal liquid are merged the extracting juice that removes slag.
7) the Hericium erinaceus (Bull. Ex Fr.) Pers. decoction made from powder granule of paper bag packing can be by step 4), 5) and 6) method directly whole decoct a drug wrapped boil after, take out paper bag and get final product with juice.
Embodiment 18
The method of preparation and use of SIWU TANG decoction made from powder particulate composition is got Radix Rehmanniae Preparata decoction made from powder granule (crossing 20 orders) 15 grams, Radix Angelicae Sinensis decoction made from powder granule (crossing 40 orders) 15 grams, Radix Paeoniae Alba decoction made from powder granule (crossing 80 orders) 10 grams, Rhizoma Chuanxiong decoction made from powder granule (crossing 40 orders) 8 grams, mix homogeneously, with paper bag or plastic bag packaging composition, SIWU TANG decoction made from powder particulate composition.
Get step 1) SIWU TANG decoction made from powder particulate composition, open plastic bag and pour container into, add 12 times of amount cold water soak 20min, straight fire boils, and boils rear slow fire boiling 10min, leaches medicinal liquid;
With step 2) the gained medicinal residues add 10 times of water gagings, and straight fire boils, and uses slow fire boiling 10min after boiling instead, leaches medicinal liquid;
With step 2) and step 3) gained medicinal liquid merge the extracting juice that removes slag.
The SIWU TANG decoction made from powder particulate composition of paper bag packing can be by step 2), 3) and 4) method directly whole decoct a drug wrapped boil after, take out paper bag and get final product with juice.
Get common decoction pieces Radix Rehmanniae Preparata 15 grams, Radix Angelicae Sinensis 15 grams, the Radix Paeoniae Alba 10 grams, Rhizoma Chuanxiong 8 grams, mix homogeneously, with paper bag or plastic bag packaging composition, SIWU TANG side.
After the SIWU TANG side 48g of common decoction pieces preparation and SIWU TANG decoction made from powder particulate composition 16g were added respectively 5 times of water gagings and decoct 30min respectively, the extracting juice that removes slag was concentrated into 50ml.In continuous one week of gastric infusion of kunming mice 0.15ml, give mice disposable restraint stress 18h when testing the 6th day.Water is prohibited in all mice fasting during this time.After restraining 18h, the mice etherization, the heart blood sampling is placed in the centrifuge tube of heparin processing, the centrifugal 5min of 5000rpm, separated plasma-20 ℃ storage is used for ALT and AST measures.The results are shown in Table 17.
The impact of table 17 on stress load mice plasma ALT and AST
| Group | N | Dosage (g/kg) | ALT（IU/L） | AST（IU/L） |
| The blank group | 10 | —— | 14.73 | 48.25 |
| Model group | 10 | —— | 44.25 | 136.32 |
| SIWU TANG side | 10 | 6 | 24.85* | 114.21* |
| SIWU TANG decoction made from powder granule | 10 | 2 | 21.25* | 109.42* |
By as seen from Table 17, tradition SIWU TANG side and SIWU TANG decoction made from powder granule all can reduce restraint stress mice plasma ALT and AST content (P＜0.05), the basic zero difference in SIWU TANG side and SIWU TANG decoction made from powder granule side confirms that the SIWU TANG decoction made from powder granular recipe of common drink 1/3 dosage and regular convention formula drug effect are substantially suitable.
Embodiment 19
SHENGMAI SAN decoction made from powder particulate composition and using method
1) get Radix Ginseng preparata's scattered seed (cross 20 orders) 9 grams, decoction made from powder granule Radix Ophiopogonis (crossing 10 orders) 9 grams, Fructus Schisandrae Chinensis decoction made from powder granule (crossing 20 orders) 6 grams, mix homogeneously, with paper bag or plastic bag packaging composition, SHENGMAI SAN decoction made from powder particulate composition.
2) get step 1) SHENGMAI SAN decoction made from powder particulate composition, open plastic bag and pour container into, add 12 times of amount cold water soak 20min, straight fire boils, and boils rear slow fire boiling 10min, leaches medicinal liquid;
3) with step 2) the gained medicinal residues add 10 times of water gagings, and straight fire boils, and uses slow fire boiling 10min after boiling instead, leaches medicinal liquid;
4) with step 2) and step 3) gained medicinal liquid merge the extracting juice that removes slag.
5) the SHENGMAI SAN decoction made from powder particulate composition of paper bag packing can be by step 2), 3) and 4) method directly whole decoct a drug wrapped boil after, take out paper bag and get final product with juice.
Get common decoction pieces Radix Ginseng 9 grams, Radix Ophiopogonis 9 gram, Fructus Schisandrae Chinensis 6 gram mix homogeneously, with paper bag or plastic bag packaging composition, SHENGMAI SAN side.
After the SHENGMAI SAN side 48g of common decoction pieces preparation and SHENGMAI SAN decoction made from powder particulate composition 16g were added respectively 5 times of water gagings and decoct 30min, the extracting juice that removes slag was concentrated into 50ml.The continuous gastric infusion of kunming mice 0.15ml, the mice nape every day subcutaneous injection D-of section galactose (normal saline is made into 5%, and injection volume is 0.25mL/10g) is 40d altogether.Since the 1st day, gastric infusion and with the volume normal saline respectively, administration every day 1 time, successive administration 30d.2h after giving D-galactose and perfusion last 1 time, mouse orbit is got blood, and anticoagulant heparin is measured antioxidation.
Table 18 couple Mouse Blood SOD, the impact of CAT vigor
Annotate: compare * P＜0.01 with model group
By as seen from Table 18, tradition SHENGMAI SAN side and SHENGMAI SAN decoction made from powder granule all can improve anti-oxidative ability of mice (P＜0.01), the basic zero difference in SHENGMAI SAN decoction pieces side and SHENGMAI SAN decoction made from powder granule side confirms that the SHENGMAI SAN decoction made from powder granular recipe of common decoction pieces 1/3 dosage and regular convention formula drug effect are substantially suitable.
Embodiment 20
MAXINGSHIGAN TANG decoction made from powder particulate composition and using method
1) get Herba Ephedrae decoction made from powder granule (crossing 40 orders) 6 grams, Semen Armeniacae Amarum decoction made from powder granule (crossing 20 orders) 9 grams, Gypsum Fibrosum decoction made from powder granule (crossing 120 orders) 24 grams, Radix Glycyrrhizae decoction made from powder granule (crossing 40 orders) 6 grams, mix homogeneously, with paper bag or plastic bag packaging composition, MAXINGSHIGAN TANG decoction made from powder particulate composition.
2) get step 1) MAXINGSHIGAN TANG decoction made from powder particulate composition, open plastic bag and pour container into, add 12 times of amount cold water soak 20min, straight fire boils, and boils rear slow fire boiling 10min, leaches medicinal liquid;
3) with step 2) the gained medicinal residues add 10 times of water gagings, and straight fire boils, and uses slow fire boiling 10min after boiling instead, leaches medicinal liquid;
4) with step 2) and step 3) gained medicinal liquid merge the extracting juice that removes slag.
5) the MAXINGSHIGAN TANG decoction made from powder particulate composition of paper bag packing can be by step 2), 3) and 4) method directly whole decoct a drug wrapped boil after, take out paper bag and get final product with juice.
Get common decoction pieces Herba Ephedrae 6 grams, Semen Armeniacae Amarum 9 grams, Gypsum Fibrosum 24 grams, Radix Glycyrrhizae 6 gram mix homogeneously, with paper bag or plastic bag packaging composition, MAXINGSHIGAN TANG side.
After the MAXINGSHIGAN TANG side 45g of common decoction pieces preparation and MAXINGSHIGAN TANG decoction made from powder particulate composition 15g were added respectively 5 times of water gagings and decoct 30min, the extracting juice that removes slag was concentrated into 50ml.In continuous one week of gastric infusion of kunming mice 0.15ml, the blank group is respectively at experiment the 1st, 7,14 and 21d Intraperitoneal injection normal saline 0.2ml; 28d begins to atomize with normal saline and sucks 30min, 60min normal saline 0.5ml gavage, 7d continuously before atomizing sucks.Model group is got the normal saline 0.2ml that contains egg protein 10 μ g, aluminium hydroxide 20mg, in the 1st, 7,14 and the 21d Intraperitoneal injection; 28d begins to atomize with the normal saline that mass fraction is 5% egg protein and sucks 30min, 60min normal saline 0.5ml gavage, 7d continuously before atomizing sucks.Each administration group mouse sensitization with excite same asthma group.60min medicine gavage before atomizing sucks, 7d continuously.Test the disconnected marrow of 35d and put to death mice, dissect and take out lungs, measure IFN-γ, IL-4.
Table 19 asthmatic mouse IFN-γ, IL-4 are relatively
| Group | N | Dosage (g/kg) | IFN-γ | IL-4 |
| The blank group | 10 | —— | 3.4248±0.9986 | 2.1675±0.2262 |
| Model group | 10 | —— | 14.3124±2.7473 | 21.9564±7.8421 |
| MAXINGSHIGAN TANG side | 10 | 6 | 25.7474±3.6481* | 4.3116±1.2099** |
| MAXINGSHIGAN TANG decoction made from powder granule | 10 | 2 | 26.2537±3.7144* | 4.1857±1.1635** |
Annotate: compare * P＜0.05**P＜0.01 with model group
By as seen from Table 19, tradition decoction pieces MAXINGSHIGAN TANG side and MAXINGSHIGAN TANG decoction made from powder granule can improve the mouse asthma inflammatory states, both the basic zero difference of drug effect, confirm that the MAXINGSHIGAN TANG decoction made from powder granular recipe of common decoction pieces 1/3 dosage and regular convention formula drug effect are substantially suitable.
Embodiment 21
Daochi powder decoction made from powder particulate composition
1) Radix Rehmanniae decoction made from powder granule (crossing 10 orders) 6 grams, Caulis Akebiae decoction made from powder granule (crossing 60 orders) 6 grams, Radix Glycyrrhizae tip decoction made from powder granule (crossing 40 orders) 6 grams, Folium Bambusae decoction made from powder granule (crossing 20 orders) 6 grams, mix homogeneously, with paper bag or plastic bag packaging composition, daochi powder decoction made from powder particulate composition.
2) get step 1) daochi powder decoction made from powder particulate composition, open plastic bag and pour container into, add 12 times of amount cold water soak 20min, straight fire boils, and boils rear slow fire boiling 10min, leaches medicinal liquid;
3) with step 2) the gained medicinal residues add 10 times of water gagings, and straight fire boils, and uses slow fire boiling 10min after boiling instead, leaches medicinal liquid;
4) with step 2) and step 3) gained medicinal liquid merge the extracting juice that removes slag.
5) the daochi powder decoction made from powder particulate composition of paper bag packing can be by step 2), 3) and 4) method directly whole decoct a drug wrapped boil after, take out paper bag and get final product with juice.
Get common decoction pieces Radix Rehmanniae 6 grams, Caulis Akebiae 6 grams, the Radix Glycyrrhizae tip 6 grams, Folium Bambusae 6 gram mix homogeneously, with paper bag or plastic bag packaging composition, daochi powder side.
After the daochi powder side 24g of common decoction pieces preparation and daochi powder decoction made from powder particulate composition 8g were added respectively 5 times of water gagings and decoct 25min, the extracting juice that removes slag was concentrated into 25ml.2 times (normal saline) of water load after water 18h can't help in the fasting of Wista rat, and capacity is 2ml/100g, and every minor tick 1h gives 1h gastric infusion after the water load, and the normal saline group gives distilled water.Each treated animal gavage capacity is 2ml/100g, observes urine amount in 2h, records 1 time every 1h.The results are shown in Table 20.
The impact of table 20 on the rat urine amount
| Group | N | Dosage (g/kg) | 1h urine amount (ml) | 2h urine amount (ml) |
| Matched group | 10 | —— | 0.46±0.91 | 0.25±0.46 |
| Daochi powder side | 10 | 3 | 1.40±0.84* | 0.91±0.69** |
| Daochi powder decoction made from powder granule | 10 | 1 | 1.37±0.71* | 0.97±0.63** |
Annotate: compare * P＜0.05**P＜0.01 with model group
By as seen from Table 20, traditional decoction pieces daochi powder side and daochi powder decoction made from powder granule can increase the mouse retention amount, and both the basic zero difference of drug effect, confirm that the daochi powder decoction made from powder granular recipe of common decoction pieces 1/3 dosage and regular convention formula drug effect are substantially suitable.
Embodiment 22
Herba Artemisiae Scopariae Decoction decoction made from powder particulate composition and using method
1) Herba Artemisiae Scopariae decoction made from powder granule (cross 10 orders) 12 grams, Fructus Gardeniae decoction made from powder granule (crossing 60 orders) 9 grams, Radix Et Rhizoma Rhei decoction made from powder granule (crossing 20 orders) 9 grams, mix homogeneously, with paper bag or plastic bag packaging composition, Herba Artemisiae Scopariae Decoction decoction made from powder particulate composition.
2) get step 1) Herba Artemisiae Scopariae Decoction decoction made from powder particulate composition, open plastic bag and pour container into, add 12 times of amount cold water soak 20min, straight fire boils, and boils rear slow fire boiling 10min, leaches medicinal liquid;
3) with step 2) the gained medicinal residues add 10 times of water gagings, and straight fire boils, and uses slow fire boiling 10min after boiling instead, leaches medicinal liquid;
4) with step 2) and step 3) gained medicinal liquid merge the extracting juice that removes slag.
5) the Herba Artemisiae Scopariae Decoction decoction made from powder particulate composition of paper bag packing can be by step 2), 3) and 4) method directly whole decoct a drug wrapped boil after, take out paper bag and get final product with juice.
Embodiment 23
Revolve and cover Haematitum soup decoction made from powder particulate composition and using method
1) Flos Inulae decoction made from powder granule (crossing 10 orders) 9g, Haematitum decoction made from powder granule (crossing 200 orders) 6g, Rhizoma Pinelliae decoction made from powder granule (crossing 20 orders) 9g, Radix Ginseng preparata's scattered seed (crossing 20 orders) 6g, Radix Glycyrrhizae Preparata decoction made from powder granule (crossing 20 orders) 9g, Fructus Jujubae decoction made from powder granule (crossing 10 orders) 12 grams, mix homogeneously, with paper bag or plastic bag packaging composition, revolve and cover Haematitum soup decoction made from powder particulate composition, add Rhizoma Zingiberis Recens 15 grams when taking, fry in shallow oil altogether.
2) get step 1) and revolve and cover Haematitum soup decoction made from powder particulate composition, open plastic bag and pour container into, add 12 times of amount cold water soak 20min, straight fire boils, and boils rear slow fire boiling 10min, leaches medicinal liquid;
3) with step 2) the gained medicinal residues add 10 times of water gagings, and straight fire boils, and uses slow fire boiling 10min after boiling instead, leaches medicinal liquid;
4) with step 2) and step 3) gained medicinal liquid merge the extracting juice that removes slag.
5) paper bag packing revolve cover Haematitum soup decoction made from powder particulate composition can be by step 2), 3) and 4) method directly whole decoct a drug wrapped boil after, take out paper bag and get final product with juice.
Embodiment 24
QIANZHENGSAN decoction made from powder particulate composition and using method
1) Rhizoma Typhonii decoction made from powder granule (cross 10 orders), each 6 grams of Bombyx Batryticatus decoction made from powder granule (crossing 40 orders), Scorpio decoction made from powder granule (crossing 60 orders) 4 grams, mix homogeneously, with paper bag or plastic bag packaging composition, QIANZHENGSAN decoction made from powder particulate composition.
2) get step 1) QIANZHENGSAN decoction made from powder particulate composition, open plastic bag and pour container into, add 12 times of amount cold water soak 20min, straight fire boils, and boils rear slow fire boiling 10min, leaches medicinal liquid;
3) with step 2) the gained medicinal residues add 10 times of water gagings, and straight fire boils, and uses slow fire boiling 10min after boiling instead, leaches medicinal liquid;
4) with step 2) and step 3) gained medicinal liquid merge the extracting juice that removes slag.
5) the QIANZHENGSAN decoction made from powder particulate composition of paper bag packing can be by step 2), 3) and 4) method directly whole decoct a drug wrapped boil after, take out paper bag and get final product with juice.
Embodiment 25
Radix Gentianae Macrophyllae Carapax Trionycis scattered seed compositions and using method
1) Carapax Trionycis (process by shortcake, with nine rib persons) decoction made from powder granule (crossing 80 orders) and each 30g of Cortex Lycii decoction made from powder granule (crossing 20 orders), Radix Gentianae Macrophyllae decoction made from powder granule (crossing 60 orders), Radix Angelicae Sinensis decoction made from powder granule (crossing 40 orders) and each 15g of Rhizoma Anemarrhenae decoction made from powder granule (crossing 20 orders), mix homogeneously, with paper bag or plastic bag packaging composition, the loose decoction made from powder particulate composition of Radix Gentianae Macrophyllae Carapax Trionycis.
2) get the loose decoction made from powder particulate composition of step 1) Radix Gentianae Macrophyllae Carapax Trionycis, open plastic bag and pour container into, add 12 times of amount cold water soak 20min, straight fire boils, and boils rear slow fire boiling 10min, leaches medicinal liquid;
3) with step 2) the gained medicinal residues add 10 times of water gagings, and straight fire boils, and uses slow fire boiling 10min after boiling instead, leaches medicinal liquid;
4) with step 2) and step 3) gained medicinal liquid merge the extracting juice that removes slag.
5) the loose decoction made from powder particulate composition of the Radix Gentianae Macrophyllae Carapax Trionycis of paper bag packing can be by step 2), 3) and 4) method directly whole decoct a drug wrapped boil after, the taking-up paper bag gets final product with juice.
Claims (11)
1. a Chinese medicine decoction made from powder particulate composition, is characterized in that, in described decoction made from powder particulate composition, the particle size range of the prepared slices of Chinese crude drugs is:
1) root, stem, Seed-fruit Chinese drugs are 10 ~ 100 orders, are preferably 20 orders;
2) floral leaf, careless class medical material are 10 ~ 50 orders, are preferably 20 orders;
3) animal drugs is 10 ~ 100 orders, is preferably 20 orders; And/or
4) Ore shellfish Class A medicine is 60 ~ 200 orders, is preferably 100 orders.
2. Chinese medicine decoction made from powder particulate composition according to claim 1, wherein:
Described, stem, Seed-fruit Chinese drugs are selected from: Radix Notoginseng, Radix Puerariae, Rhizoma Coptidis, the Radix Astragali, Ramulus Cinnamomi, Semen Nelumbinis, Fructus Corni, Radix Salviae Miltiorrhizae, Hericium erinaceus (Bull. Ex Fr.) Pers., Radix Rehmanniae Preparata, Radix Angelicae Sinensis, the Radix Paeoniae Alba, Rhizoma Chuanxiong, Radix Ginseng, Radix Ophiopogonis, Radix Glycyrrhizae, Radix Rehmanniae, Radix Et Rhizoma Rhei, the Rhizoma Pinelliae, Cortex Lycii, Radix Gentianae Macrophyllae, the Rhizoma Anemarrhenae, Caulis Akebiae, Rhizoma Typhonii, Fructus Schisandrae Chinensis, Fructus Gardeniae, Fructus Jujubae and Semen Armeniacae Amarum;
Described floral leaf, careless class medical material are selected from: Herba Hedyotidis Diffusae, Flos Lonicerae, Folium Perillae, Flos Inulae, Folium Bambusae, Herba Ephedrae and Herba Artemisiae Scopariae;
Described animal drugs is selected from: Pheretima, Placenta Hominis, Hirudo, Serpentis, Bombyx Batryticatus and Scorpio; And/or
Described Ore shellfish Class A medicine is selected from: Carapax Et Plastrum Testudinis, Talcum, Concha Ostreae, Gypsum Fibrosum, Haematitum and Carapax Trionycis.
3. Chinese medicine decoction made from powder particulate composition according to claim 1 and 2, wherein, described decoction made from powder particulate composition becomes the fractional pack of 1 ~ 10 gram or the large of 500 ~ 1000 grams to pack with non-woven fabrics, paper bag or plastic bag packaging; The Chinese medicine decoction made from powder particulate composition that contains 1 gram, 2 grams, 3 grams, 5 grams, 6 grams or 10 grams in preferred single fractional pack.
4. the preparation method of the described Chinese medicine decoction made from powder of claim 1 or 2 particulate composition, is characterized in that, the prepared slices of Chinese crude drugs of described each composition medical material are crushed to the particle size range described in claim 1.
5. preparation method according to claim 4, further comprise described Chinese medicine decoction made from powder particulate composition is become 1 ~ 10 gram with non-woven fabrics, paper bag or plastic bag packaging, the fractional pack of preferred 1 gram, 2 grams, 3 grams, 5 grams, 6 grams or 10 grams, or be packaged into the potion recipe quantity by each prescription composition, or be packaged into the large packing of 500 ~ 1000 grams.
6. the decocting method of the described Chinese medicine decoction made from powder of any one particulate composition in claims 1 to 3 comprises the following steps:
1) get described decoction made from powder particulate composition and pour container into or with the non-woven bag parcel, add cold water soak, then straight fire boils, and with slow fire boiling, leaches medicinal liquid after boiling;
2) add entry in step 1) gained medicinal residues, straight fire boils, and uses slow fire boiling instead after boiling, and leaches medicinal liquid;
3) with step 1) and step 2) merging of gained medicinal liquid, and get final product.
7. method according to claim 6, is characterized in that, is incorporated as the cold water soak of 8 ~ 12 times of weight of described decoction made from powder particulate composition weight in described step 1).
8. according to claim 6 or 7 described methods, is characterized in that, soaks 20 ~ 60min after adding cold water in described step 1).
9. the described method of any one according to claim 6-8, is characterized in that, in described step 1), the slow fire decocting time is 20 ~ 40min.
10. the described method of any one according to claim 6-9, is characterized in that described step 2) in be incorporated as the water of 6 ~ 10 times of weight of decoction made from powder particle weight; Preferably, described step 2), the slow fire decocting time is 10 ~ 30min.
11. the relation between the using dosage of the using dosage of the described Chinese medicine decoction made from powder of claim 1 or 2 particulate composition and the prepared slices of Chinese crude drugs of identical component, it is characterized in that, the using dosage of described Chinese medicine decoction made from powder particulate composition is 30 % by weight ~ 40 % by weight of using dosage of the prepared slices of Chinese crude drugs of identical component; Preferably, the using dosage of described Chinese medicine decoction made from powder particulate composition be identical component the prepared slices of Chinese crude drugs using dosage 1/3.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103704833A (en) * | 2013-12-13 | 2014-04-09 | 青岛安信医疗器械有限公司 | Traditional Chinese medicine beverage capable of clearing internal heat and relieving summer heat, and preparation method thereof |
| CN108078913A (en) * | 2017-12-26 | 2018-05-29 | 重庆希尔安药业有限公司 | Fluonex and preparation method thereof |
| CN110870897A (en) * | 2018-08-30 | 2020-03-10 | 湖北香连药业有限责任公司 | Cortex lycii radicis formula particle and preparation method and application thereof |
| CN113559135A (en) * | 2021-09-01 | 2021-10-29 | 王建波 | Preparation method of round brick boiling powder decoction pieces with uniform quality |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1193511A (en) * | 1997-03-19 | 1998-09-23 | 张敖何 | Manufacture of Chinese medicinal pills for prescriptions |
| CN1679888A (en) * | 2005-02-03 | 2005-10-12 | 徐云裳 | Decoction Chinese medicine sterilizing antiphlogistic, preparation and use thereof |
| US8202553B2 (en) * | 2009-12-31 | 2012-06-19 | Guihua Lan | Methods and compositions for treating blood circulation disorders |
-
2013
- 2013-01-25 CN CN2013100302717A patent/CN103099788A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1193511A (en) * | 1997-03-19 | 1998-09-23 | 张敖何 | Manufacture of Chinese medicinal pills for prescriptions |
| CN1679888A (en) * | 2005-02-03 | 2005-10-12 | 徐云裳 | Decoction Chinese medicine sterilizing antiphlogistic, preparation and use thereof |
| US8202553B2 (en) * | 2009-12-31 | 2012-06-19 | Guihua Lan | Methods and compositions for treating blood circulation disorders |
Non-Patent Citations (1)
| Title |
|---|
| 董霄汉等: "中药煮散的临床应用研究(五)- 药材不同粒度入煎总煎出物的比较", 《河南中医》, vol. 10, no. 1, 31 December 1990 (1990-12-31) * |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103704833A (en) * | 2013-12-13 | 2014-04-09 | 青岛安信医疗器械有限公司 | Traditional Chinese medicine beverage capable of clearing internal heat and relieving summer heat, and preparation method thereof |
| CN108078913A (en) * | 2017-12-26 | 2018-05-29 | 重庆希尔安药业有限公司 | Fluonex and preparation method thereof |
| CN110870897A (en) * | 2018-08-30 | 2020-03-10 | 湖北香连药业有限责任公司 | Cortex lycii radicis formula particle and preparation method and application thereof |
| CN110870897B (en) * | 2018-08-30 | 2022-03-25 | 湖北香连药业有限责任公司 | Preparation method of cortex lycii radicis formula particles |
| CN113559135A (en) * | 2021-09-01 | 2021-10-29 | 王建波 | Preparation method of round brick boiling powder decoction pieces with uniform quality |
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