CN103081810A - Composite cotton and hemp stalk bioactive padding and preparation method thereof - Google Patents
Composite cotton and hemp stalk bioactive padding and preparation method thereof Download PDFInfo
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Images
Abstract
The invention provides composite cotton and hemp straw bioactive padding and a preparation method thereof. The composite cotton and hemp stalk bioactive padding comprises a cotton stalk crushed material, a ramie stalk crushed material, a composite microbial agent and water, wherein the composite cotton and hemp stalk bioactive padding has the advantages of: 1) efficiently decomposing excrements of livestock and poultry; 2) improving resistance of livestock and poultry; 3) speeding up growth speed of livestock and poultry; and 4) being used for a long time, generally for 4-5 years, and promoting sustainable development of breeding industry as the time and effect of the cotton and hemp stalk bioactive padding are 2-3 times higher than the time and effect of a common biological padding.
Description
Technical field
The present invention relates to the microbial fermentation field, relate in particular to a kind of composite cotton fabric waste of flax biologically active bedding and padding that utilize microbial fermentation to make, and its preparation method.
Background technology
General biological pad main component is bacterial classification, and peanut shell, maize straw and straw etc., its preparation method are by with peanut shell, and maize straw and straw first mix, and then adds a certain amount of bacterial classification and drinking water to be mixed with to form.The bacterial classification that uses is generally Bacillus natto, bacillus licheniformis.But this biological pad service time not very long, easily rot; Decompose DeGrain.
Summary of the invention
The object of the invention is to overcome the defective of existing biological pad, providing a kind of can use for a long time, NEW TYPE OF COMPOSITE linen-cotton bar biologically active bedding and padding and its preparation method that capacity of decomposition is higher.
Another object of the present invention also is to provide a kind of a kind of complex micro organism fungicide that uses in described composite cotton fabric waste of flax biologically active bedding and padding, and its preparation method.
To achieve these goals, a kind of complex micro organism fungicide provided by the invention is that mixed culture fermentation liquid by bacillus subtilis and saccharomyces cerevisiae is adsorbed onto on analysis for soybean powder and makes; Effective total viable count of described mixed culture fermentation liquid is hundred million/ml of 80-100, and wherein the living bacteria count of bacillus subtilis is 70-80%, and the living bacteria count of saccharomyces cerevisiae is 20-30%; Described mixed culture fermentation liquid with the volume mass ratio of analysis for soybean powder is: (30-40): (1-2).
Wherein, described mixed culture fermentation liquid is preferably with the volume mass ratio of analysis for soybean powder: 35: 1.
The volume mass ratio of above-mentioned mixed culture fermentation liquid and analysis for soybean powder is according to L: g calculates.
Described complex micro organism fungicide can also comprise other carriers, as corn flour or wheat bran etc.
The preparation method of complex micro organism fungicide of the present invention, saccharomyces cerevisiae, bacillus subtilis to be carried out respectively the inclined-plane cultivate, first order seed is cultivated and secondary seed is cultivated, then carries out adsorbing analysis for soybean powder after mixing fermentation culture in fermentation tank, obtains described complex micro organism fungicide.
Particularly, comprise the steps:
1) inclined-plane is cultivated, and comprises saccharomyces cerevisiae, bacillus subtilis original strain are inoculated on solid culture medium respectively and cultivate;
2) first order seed is cultivated, and comprises step 1) saccharomyces cerevisiae, each bacterial classification of bacillus subtilis of cultivating be inoculated in respectively on medium, makes the first order seed of saccharomyces cerevisiae, bacillus subtilis;
3) secondary seed is cultivated, and comprises step 2) saccharomyces cerevisiae, the bacillus subtilis first order seed cultivated be inoculated into respectively in the fermentation tank that contains medium and cultivate, and makes the secondary seed of saccharomyces cerevisiae, bacillus subtilis;
4) mixing fermentation culture comprises step 3) saccharomyces cerevisiae, the bacillus subtilis secondary seed cultivated be inoculated in the fermentation tank that contains culture fluid one by one, carry out high density fermentation cultivate after the absorption analysis for soybean powder, obtain complex micro organism fungicide of the present invention.
Wherein, step 1) during the inclined-plane was cultivated, the solid culture medium of described saccharomyces cerevisiae, bacillus subtilis was: beef extract 3g, and peptone 10g, NaCl 5g, agar 18g adds water to 1000ml.
Wherein, step 1) during the inclined-plane is cultivated, will cultivate 3 days under 30 ℃ of conditions of saccharomyces cerevisiae.
Wherein, step 1) during the inclined-plane is cultivated, will cultivate 2 days under 37 ℃ of conditions of bacillus subtilis.
Wherein, step 2) during first order seed was cultivated, the medium of described saccharomyces cerevisiae was: tryptone 10g, and yeast extract 5g, sodium chloride 5g adds water to 1000ml.
Wherein, step 2) during first order seed was cultivated, the medium of described bacillus subtilis was: beef extract 3g, and peptone 10g, NaCl 5g, agar 18g adds water to 1000ml.
Wherein, step 2) during first order seed was cultivated, under 30 ℃ of conditions of saccharomyces cerevisiae, the 150r/min shaking table was cultivated 3 days.
Wherein, step 2) during first order seed was cultivated, under 37 ℃ of conditions of bacillus subtilis, static cultivation was 2 days.
Wherein, step 2) during first order seed was cultivated, when finishing to cultivate, saccharomyces cerevisiae, each bacteria suspension optical density (OD600) value of bacillus subtilis all reached 4.0, and surperficial growth situation is better.
Wherein, step 3) during secondary seed was cultivated, the inoculum concentration of first order seed was step 2) described liquid nutrient medium volume 10%.
Wherein, step 3) during secondary seed was cultivated, in fermentation tank, the medium of saccharomyces cerevisiae was tryptone 10g, yeast extract 5g, and sodium chloride 5g adds water to 1000ml.
Wherein, step 3) during secondary seed was cultivated, in fermentation tank, the medium of bacillus subtilis was beef extract 3g, peptone 10g, and NaCl 5g, agar 18g adds water to 1000ml.
Wherein, step 3) during secondary seed was cultivated, in fermentation tank, the cumulative volume of culture fluid was 60L.
Wherein, step 3) during secondary seed was cultivated, under 30 ℃ of conditions of saccharomyces cerevisiae, mixing speed was 150r/min, and throughput is 1: 1, cultivates 3 days.
Wherein, step 3) during secondary seed is cultivated, cultivated 2 days under 37 ℃ of conditions of bacillus subtilis.
Wherein, step 4) in mixing fermentation culture, the inoculum concentration of secondary seed is step 3) described liquid nutrient medium volume 15%.
Wherein, step 4) in mixing fermentation culture, analysis for soybean powder joins in culture fluid in fermentation tank as adsorbent, and the quality of each composition of culture fluid and analysis for soybean powder is to calculate on their gross mass basis.In saccharomyces cerevisiae, bacillus subtilis bacteria culture fluid, the mass percent of each composition and analysis for soybean powder is: analysis for soybean powder 20%, and brown sugar 1-3%, peptone 0.1-0.5% and wheat bran 30%, surplus is water.
The culture fluid of this step saccharomyces cerevisiae, bacillus subtilis can also be tryptone 10g, yeast extract 5g, and sodium chloride 5g adds water to 1000ml; Similarly, adding the quality of analysis for soybean powder is 20g.
Wherein, step 4) in mixing fermentation culture, the culture fluid cumulative volume in fermentation tank is 600-700L.
Wherein, step 4) in mixing fermentation culture, concrete high density fermentation is cultivated and is adopted the batch feeding training method, and wherein feed supplement carbon source is: glucose, glycerine, sucrose or brown sugar; Nitrogenous source is: ammonium sulfate or peptone.
Wherein, the quality of described supplementary carbon source is 5-10g, preferred 8g.
Wherein, the quality of described additional nitrogenous source is 1-2g, preferred 2g.
Wherein, step 4) the mixing fermentation culture process comprises: a, aerobic cultivation stage; B, slightly soluble oxygen and anaerobism cultivation stage.
Wherein, step 4) in mixing fermentation culture, the ratio of bacillus subtilis secondary seed is 70-80%, and the secondary seed ratio of saccharomyces cerevisiae is 20-30%.
The preparation method of above-mentioned complex micro organism fungicide can also comprise concentrated and spray-dired processing, carries out after mixing fermentation culture.
Composite cotton fabric waste of flax biologically active bedding and padding of the present invention comprise: cotton stem crushed material, ramie stalk crushed material, described complex micro organism fungicide and water.
Wherein, in the composite cotton fabric waste of flax biologically active bedding and padding of every cubic metre, described cotton stem crushed material, ramie stalk crushed material, the mass ratio of water and complex micro organism fungicide are (4-5): (3-4): 1.9: 0.1.
Wherein, described water can use drinking water.
Wherein, the thickness of described composite cotton fabric waste of flax biologically active bedding and padding is 80cm-90cm.
A kind of preparation method of composite cotton fabric waste of flax biologically active bedding and padding comprises: first the preparation method according to described complex micro organism fungicide obtains complex micro organism fungicide, then adds respectively ramie stalk crushed material, cotton stem crushed material and water mixing manufacture to form.
Wherein, in the preparation process of composite cotton fabric waste of flax biologically active bedding and padding, described ramie stalk crushed material, the cotton stem crushed material that adds is to be pulverized and obtained by ramie stalk, cotton stem respectively.The consumption of each component that adds is with the corresponding restriction in the described composite cotton fabric waste of flax of content of the present invention biologically active bedding and padding.The water that adds is that drinking water gets final product.
The present invention adopts biological inoculum (saccharomyces cerevisiae and bacillus subtilis), inoculate bacillus subtilis in take analysis for soybean powder as main fermentation liquid material during mixing fermentation culture, saccharomyces cerevisiae ferments, with mixed culture fermentation liquid absorption analysis for soybean powder and be deployed into pure bacterium liquid after processing and store.And then add ramie stalk crushed material, cotton stem crushed material and water to mix.
Its preparation technology's flow process is as follows:
Inclined-plane cultivation → shaker fermentation (first order seed cultivate and secondary seed cultivate) → mixing fermentation culture → mixed culture fermentation liquid absorption analysis for soybean powder and process (concentrating and atomized drying) → allotment → can → sterilize → store → add ramie stalk crushed material, cotton stem crushed material and water.Wherein, after the mixed culture fermentation liquid of absorption analysis for soybean powder is processed, the sequencing that adds material is not required.
Select bacillus subtilis, saccharomyces cerevisiae bacterial classification as fermented bacterium, first select the bacterial classification of separate sources, by separation and purification, and fermentability is compared, then select bacillus subtilis, the saccharomyces cerevisiae bacterial classification that to make.
The present invention is after atomized drying, and the preservation condition of mixed culture fermentation liquid and correlation index are:
Temperature: 30 ℃-37 ℃; Time: 2 days-4 days; Effective hundred million/ml of total viable count: 80-100, wherein that bacillus subtilis is 70-80%, saccharomyces cerevisiae be 20-30%; PH:6.0-7.0; Check: no wonder smell, the dull and stereotyped cultivation without any miscellaneous bacteria.
Key problem in technology point of the present invention is:
1, new microbial inoculum, i.e. complex micro organism fungicide have been adopted;
2, avoided the fermentation condition under high temperature;
3, the long-acting raw material of high-quality have been adopted;
4, service time is long;
5, capacity of decomposition is strong.
Therefore, the invention provides a kind of active bedding and padding of the biofermentation that can use for a long time in column home that cultivate, its effect has: 1, can effectively decompose the excreta of livestock and poultry, 2, improve the resistance of livestock and poultry body, 3, accelerate the growth rate of livestock and poultry, 4, can use for a long time, be generally 4-5, time and effect are all than the high 2-3 of general biological pad doubly; On the whole, can promote the sustainable development of aquaculture.
Description of drawings
Fig. 1 is composite cotton fabric waste of flax biologically active bedding and padding production technological process.
Embodiment
Following examples are used for explanation the present invention, but are not used for limiting the scope of the invention.
The present invention's saccharomyces cerevisiae used, bacillus subtilis original strain derive from: independent development, screening in cow dung, purification, rejuvenation.
Embodiment 1
1, the preparation of complex micro organism fungicide.
1) inclined-plane is cultivated: will be inoculated in respectively on solid culture medium under saccharomyces cerevisiae, each 10g aseptic condition of bacillus subtilis original strain, will cultivate 3 days under 30 ℃ of conditions of saccharomyces cerevisiae, cultivated 2 days under 37 ℃ of conditions of bacillus subtilis; The solid culture medium of saccharomyces cerevisiae, bacillus subtilis is: beef extract 3g, and peptone 10g, NaCl 5g, agar 18g adds water to 1000ml;
2) be inoculated in respectively medium under the bacterial classification aseptic condition of first order seed cultivation: with step 1) cultivating, under 30 ℃ of conditions of saccharomyces cerevisiae, the 150r/min shaking table was cultivated 3 days, under 37 ℃ of conditions of bacillus subtilis, static cultivation is 2 days, make first order seed, when finishing to cultivate, saccharomyces cerevisiae, each bacteria suspension optical density of bacillus subtilis OD600 value all reach 4.0; The medium of saccharomyces cerevisiae is: tryptone 10g, and yeast extract 5g, sodium chloride 5g adds water to 1000ml; The medium of bacillus subtilis is: beef extract 3g, and peptone 10g, NaCl 5g, agar 18g adds water to 1000ml.
3) secondary seed is cultivated: be 10% inoculum concentration by the volume ratio of liquid nutrient medium, first order seed is inoculated into respectively in the fermentation tank of 100L, in fermentation tank, the cumulative volume of medium is 60L, under 30 ℃ of conditions of saccharomyces cerevisiae, mixing speed is 150r/min, and throughput is 1: 1, cultivates 3 days, cultivated 2 days under 37 ℃ of conditions of bacillus subtilis, make secondary seed; In fermentation tank, the medium of saccharomyces cerevisiae is tryptone 10g, yeast extract 5g, and sodium chloride 5g adds water to 1000ml; The medium of bacillus subtilis is beef extract 3g, peptone 10g, and NaCl 5g, agar 18g adds water to 1000ml;
4) mixing fermentation culture: be 15% inoculum concentration by the volume ratio of liquid nutrient medium, secondary seed is inoculated into one by one in the fermentation tank of 1 ton (the secondary seed ratio of bacillus subtilis and saccharomyces cerevisiae is 7: 3), culture fluid cumulative volume in fermentation tank is 700L, carry out high density fermentation and cultivate, obtain complex micro organism fungicide; The medium that saccharomyces cerevisiae, bacillus subtilis are used and the analysis for soybean powder formula that adds are by mass percentage: analysis for soybean powder 20%, and brown sugar 2%, peptone 0.3% and wheat bran 30%, surplus is water.
Above-mentioned concrete high density fermentation is cultivated and is adopted the batch feeding training method, and wherein supplementary carbon source is in the culture fluid of 700L: glucose 8g; Nitrogenous source is: ammonium sulfate 2g.The batch feeding training method refer in fermentation process with certain specifically placing restrictions on property bottoms stream be added in reactor, the mode of operation that the purpose product just extracts in the time of will be to results.
The mixing fermentation culture process comprises: a, aerobic cultivation stage: in initial 0-24 hour, the interval ventilation remains on the aerobic conditions fermentation, throughput 1: 1.2, regulation and control fermentation dissolved oxygen 10%, speed of agitator 180r/min, 2 hours mixing chamber intervals, stirred 30 ℃ of temperature 2 minutes; B, slightly soluble oxygen and anaerobism cultivation stage: 60 hours, keep zymotic fluid upper strata slightly soluble oxygen condition, static cultivation, stir at the interval, 4 hours mixing chamber intervals, stirred use sulfate sulfatase ammonium water modification stability pH 4.0,30 ℃ of temperature 4 minutes.
2, the preparation of composite cotton fabric waste of flax biologically active bedding and padding.
With the complex micro organism fungicide that obtains, add respectively ramie stalk crushed material, cotton stalk powder to mince and the water mixing manufacture forms, its proportioning is: every cubic metre adds cotton stem 50kg, ramie stalk 30kg, 19kg and complex micro organism fungicide 1kg.The biological pad thickness that makes is 80cm.
The implementation result tables of data:
Show from the experiment effect data, the biologically active bedding and padding of the present invention made from the linen-cotton bar are stronger than common biological pad fecaluria capacity of decomposition, long 2-3 times of service time.
Claims (10)
1. a complex micro organism fungicide, is characterized in that, is that mixed culture fermentation liquid by bacillus subtilis and saccharomyces cerevisiae is adsorbed onto on analysis for soybean powder and makes; Effective total viable count of described mixed culture fermentation liquid is hundred million/ml of 80-100, and wherein the living bacteria count of bacillus subtilis is 70-80%, and the living bacteria count of saccharomyces cerevisiae is 20-30%; Described mixed culture fermentation liquid with the volume mass ratio of analysis for soybean powder is: (30-40): (1-2).
2. the preparation method of complex micro organism fungicide claimed in claim 1, it is characterized in that, comprise that saccharomyces cerevisiae, bacillus subtilis are carried out respectively the inclined-plane to be cultivated, first order seed is cultivated and secondary seed is cultivated, carry out again adsorbing analysis for soybean powder after mixing fermentation culture in fermentation tank, obtain described complex micro organism fungicide.
3. the preparation method of complex micro organism fungicide according to claim 2, it is characterized in that, in described mixing fermentation culture, in culture fluid in fermentation tank, the mass percent of each composition and analysis for soybean powder is: analysis for soybean powder 20%, brown sugar 1-3%, peptone 0.1-0.5%, wheat bran 30%, surplus is water; Be perhaps: tryptone 10g, yeast extract 5g, sodium chloride 5g, analysis for soybean powder 20g adds water to 1000ml.
4. the preparation method of complex micro organism fungicide according to claim 2, is characterized in that, during described inclined-plane was cultivated, the medium of saccharomyces cerevisiae, bacillus subtilis was: beef extract 3g, and peptone 10g, NaCl 5g, agar 18g adds water to 1000ml.
5. the preparation method of complex micro organism fungicide according to claim 2, is characterized in that, during described first order seed was cultivated, the medium of saccharomyces cerevisiae was: tryptone 10g, and yeast extract 5g, sodium chloride 5g adds water to 1000ml; The medium of bacillus subtilis is: beef extract 3g, and peptone 10g, NaCl 5g, agar 18g adds water to 1000ml.
6. the preparation method of complex micro organism fungicide according to claim 2, is characterized in that, during described secondary seed was cultivated, the medium of saccharomyces cerevisiae was tryptone 10g, yeast extract 5g, and sodium chloride 5g adds water to 1000ml; The medium of bacillus subtilis is beef extract 3g, peptone 10g, and NaCl 5g, agar 18g adds water to 1000ml.
7. composite cotton fabric waste of flax biologically active bedding and padding, is characterized in that, comprising: cotton stem crushed material, ramie stalk crushed material, complex micro organism fungicide claimed in claim 1 and water.
8. composite cotton fabric waste of flax biologically active bedding and padding according to claim 7, it is characterized in that, in the composite cotton fabric waste of flax biologically active bedding and padding of every cubic metre, described cotton stem crushed material, ramie stalk crushed material, the mass ratio of water and complex micro organism fungicide are (4-5): (3-4): 1.9: 0.1.
9. according to claim 7 or 8 described composite cotton fabric waste of flax biologically active bedding and padding, is characterized in that, the thickness of described composite cotton fabric waste of flax biologically active bedding and padding is 80cm-90cm.
10. the preparation method of the described composite cotton fabric waste of flax of claim 7-9 any one biologically active bedding and padding, comprise: first the preparation method according to the described complex micro organism fungicide of claim 2-6 any one obtains complex micro organism fungicide, then adds respectively ramie stalk crushed material, cotton stem crushed material and water mixing manufacture to form.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201110346659.9A CN103081810B (en) | 2011-11-04 | 2011-11-04 | Composite cotton and hemp stalk bioactive padding and preparation method thereof |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201110346659.9A CN103081810B (en) | 2011-11-04 | 2011-11-04 | Composite cotton and hemp stalk bioactive padding and preparation method thereof |
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| Publication Number | Publication Date |
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| CN103081810A true CN103081810A (en) | 2013-05-08 |
| CN103081810B CN103081810B (en) | 2014-11-26 |
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| CN112205307A (en) * | 2020-10-12 | 2021-01-12 | 山东汇邦渤海农业开发有限公司 | Bedding layer for dairy cow breeding and manufacturing and using method thereof |
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| CN103081810B (en) | 2014-11-26 |
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