CN103080326A - 用于从蔗糖产生甘油和甘油衍生产物的重组细菌 - Google Patents

用于从蔗糖产生甘油和甘油衍生产物的重组细菌 Download PDF

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Publication number
CN103080326A
CN103080326A CN201080002241XA CN201080002241A CN103080326A CN 103080326 A CN103080326 A CN 103080326A CN 201080002241X A CN201080002241X A CN 201080002241XA CN 201080002241 A CN201080002241 A CN 201080002241A CN 103080326 A CN103080326 A CN 103080326A
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China
Prior art keywords
seq
sucrose
polypeptide
glycerol
activity
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Pending
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CN201080002241XA
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English (en)
Chinese (zh)
Inventor
A·C·埃利奥特
A·A·加滕比
T·K·范戴克
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EIDP Inc
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EI Du Pont de Nemours and Co
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Publication of CN103080326A publication Critical patent/CN103080326A/zh
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2451—Glucanases acting on alpha-1,6-glucosidic bonds
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1205—Phosphotransferases with an alcohol group as acceptor (2.7.1), e.g. protein kinases
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/18—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic polyhydric
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/18—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic polyhydric
    • C12P7/20—Glycerol
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
    • C12P7/42—Hydroxy-carboxylic acids

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
CN201080002241XA 2009-12-04 2010-12-03 用于从蔗糖产生甘油和甘油衍生产物的重组细菌 Pending CN103080326A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US26660509P 2009-12-04 2009-12-04
US61/266,605 2009-12-04
PCT/US2010/058832 WO2011069033A1 (en) 2009-12-04 2010-12-03 Recombinant bacteria for producing glycerol and glycerol-derived products from sucrose

Publications (1)

Publication Number Publication Date
CN103080326A true CN103080326A (zh) 2013-05-01

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Family Applications (1)

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CN201080002241XA Pending CN103080326A (zh) 2009-12-04 2010-12-03 用于从蔗糖产生甘油和甘油衍生产物的重组细菌

Country Status (10)

Country Link
US (1) US20110136190A1 (enExample)
EP (1) EP2507377A1 (enExample)
JP (1) JP2013512675A (enExample)
KR (1) KR20120099116A (enExample)
CN (1) CN103080326A (enExample)
AU (1) AU2010325895B2 (enExample)
BR (1) BR112012012719A2 (enExample)
CA (1) CA2780757A1 (enExample)
SG (1) SG181152A1 (enExample)
WO (1) WO2011069033A1 (enExample)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103620026A (zh) * 2010-12-06 2014-03-05 纳幕尔杜邦公司 具有代谢蔗糖能力的重组细菌

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8629243B2 (en) 2011-08-16 2014-01-14 E I Du Pont De Nemours And Company Variant sucrose transporter polypeptides that enable faster sucrose utilization in bacteria
US8673602B2 (en) * 2011-08-16 2014-03-18 E I Du Pont De Nemours And Company Recombinant bacteria having improved sucrose utilization
US8686114B2 (en) * 2012-03-05 2014-04-01 E I Du Pont De Nemours And Company Variant sucrose transporter polypeptides
US9017961B2 (en) * 2012-03-05 2015-04-28 E.I. Du Pont De Nemours And Company Recombinant bacteria comprising novel sucrose transporters

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1239511A (zh) * 1996-11-13 1999-12-22 金克克国际有限公司 1,3－丙二醇的重组生产方法
EP1149911A2 (en) * 2000-04-26 2001-10-31 Ajinomoto Co., Ltd. Amino acid producing strains belonging to the genus Escherichia and method for producing amino acid

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US5686276A (en) 1995-05-12 1997-11-11 E. I. Du Pont De Nemours And Company Bioconversion of a fermentable carbon source to 1,3-propanediol by a single microorganism
EP0948628B1 (en) * 1996-11-13 2010-06-23 E.I. Du Pont De Nemours And Company Method for the production of glycerol by recombinant organisms
AU1619199A (en) 1997-12-02 1999-06-16 E.I. Du Pont De Nemours And Company Method for the production of glycerol by recombinant organisms
DK1204755T3 (da) 1999-08-18 2006-12-11 Du Pont Proces til biologisk produktion af 1,3-propandiol
CN100366730C (zh) 2002-04-22 2008-02-06 纳幕尔杜邦公司 用于遗传工程的启动子和质粒系统
ATE493490T1 (de) 2002-10-04 2011-01-15 Du Pont Verfahren zur biologischen herstellung von 1,3- propandiol mit hoher ausbeute
CA2500741C (en) * 2002-10-04 2017-02-28 Genencor International, Inc. Glucose transport mutants for production of biomaterial
US7524660B2 (en) 2005-05-05 2009-04-28 E.I. Du Pont De Nemours And Company Utilization of fructose in microbial production strains
US9051588B2 (en) * 2007-03-23 2015-06-09 Metabolic Explorer Microorganisms and methods for production of 1,2-propanediol and acetol
BRPI0823256B8 (pt) * 2008-11-07 2020-05-26 Metabolic Explorer Sa uso de sacarose como substrato para produção fermentativa de 1,2-propanodiol

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1239511A (zh) * 1996-11-13 1999-12-22 金克克国际有限公司 1,3－丙二醇的重组生产方法
US6013494A (en) * 1996-11-13 2000-01-11 E. I. Du Pont De Nemours And Company Method for the production of 1,3-propanediol by recombinant microorganisms
EP1149911A2 (en) * 2000-04-26 2001-10-31 Ajinomoto Co., Ltd. Amino acid producing strains belonging to the genus Escherichia and method for producing amino acid

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
KUNT JAHREIS ET AL.: "Adaptation of Sucrose Metabolism in the Escherichia coli Wild-Type Strain EC3132", 《JOURNAL OF BACTERIOLOGY》 *
R.K.SAXENA ET AL.: "Microbial production of 1,3-propanediol: Recent developments and emerging opportunities", 《BIOTECHNOLOGY ADVANCES》 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103620026A (zh) * 2010-12-06 2014-03-05 纳幕尔杜邦公司 具有代谢蔗糖能力的重组细菌

Also Published As

Publication number Publication date
WO2011069033A1 (en) 2011-06-09
BR112012012719A2 (pt) 2015-09-29
AU2010325895A1 (en) 2012-05-31
AU2010325895B2 (en) 2014-11-06
SG181152A1 (en) 2012-07-30
CA2780757A1 (en) 2011-06-09
EP2507377A1 (en) 2012-10-10
JP2013512675A (ja) 2013-04-18
US20110136190A1 (en) 2011-06-09
KR20120099116A (ko) 2012-09-06

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Application publication date: 20130501