CA2780757A1 - Recombinant bacteria for producing glycerol and glycerol-derived products from sucrose - Google Patents

Recombinant bacteria for producing glycerol and glycerol-derived products from sucrose Download PDF

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Publication number
CA2780757A1
CA2780757A1 CA2780757A CA2780757A CA2780757A1 CA 2780757 A1 CA2780757 A1 CA 2780757A1 CA 2780757 A CA2780757 A CA 2780757A CA 2780757 A CA2780757 A CA 2780757A CA 2780757 A1 CA2780757 A1 CA 2780757A1
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CA
Canada
Prior art keywords
seq
sucrose
glycerol
set forth
polypeptide
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA2780757A
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English (en)
French (fr)
Inventor
Andrew C. Eliot
Anthony A. Gatenby
Tina K. Van Dyk
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
EIDP Inc
Original Assignee
EI Du Pont de Nemours and Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by EI Du Pont de Nemours and Co filed Critical EI Du Pont de Nemours and Co
Publication of CA2780757A1 publication Critical patent/CA2780757A1/en
Abandoned legal-status Critical Current

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20Bacteria; Culture media therefor
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/24Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405Glucanases
    • C12N9/2451Glucanases acting on alpha-1,6-glucosidic bonds
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/11DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52Genes encoding for enzymes or proenzymes
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10Transferases (2.)
    • C12N9/12Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1205Phosphotransferases with an alcohol group as acceptor (2.7.1), e.g. protein kinases
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00Preparation of oxygen-containing organic compounds
    • C12P7/02Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00Preparation of oxygen-containing organic compounds
    • C12P7/02Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/18Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic polyhydric
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00Preparation of oxygen-containing organic compounds
    • C12P7/02Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/18Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic polyhydric
    • C12P7/20Glycerol
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00Preparation of oxygen-containing organic compounds
    • C12P7/40Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
    • C12P7/42Hydroxy-carboxylic acids

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
CA2780757A 2009-12-04 2010-12-03 Recombinant bacteria for producing glycerol and glycerol-derived products from sucrose Abandoned CA2780757A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US26660509P 2009-12-04 2009-12-04
US61/266,605 2009-12-04
PCT/US2010/058832 WO2011069033A1 (en) 2009-12-04 2010-12-03 Recombinant bacteria for producing glycerol and glycerol-derived products from sucrose

Publications (1)

Publication Number Publication Date
CA2780757A1 true CA2780757A1 (en) 2011-06-09

Family

ID=43499925

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2780757A Abandoned CA2780757A1 (en) 2009-12-04 2010-12-03 Recombinant bacteria for producing glycerol and glycerol-derived products from sucrose

Country Status (10)

Country Link
US (1) US20110136190A1 (enExample)
EP (1) EP2507377A1 (enExample)
JP (1) JP2013512675A (enExample)
KR (1) KR20120099116A (enExample)
CN (1) CN103080326A (enExample)
AU (1) AU2010325895B2 (enExample)
BR (1) BR112012012719A2 (enExample)
CA (1) CA2780757A1 (enExample)
SG (1) SG181152A1 (enExample)
WO (1) WO2011069033A1 (enExample)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8129170B1 (en) * 2010-12-06 2012-03-06 E.I. Du Pont De Nemours And Company Recombinant bacteria having the ability to metabolize sucrose
US8673602B2 (en) * 2011-08-16 2014-03-18 E I Du Pont De Nemours And Company Recombinant bacteria having improved sucrose utilization
US8629243B2 (en) 2011-08-16 2014-01-14 E I Du Pont De Nemours And Company Variant sucrose transporter polypeptides that enable faster sucrose utilization in bacteria
US9017961B2 (en) * 2012-03-05 2015-04-28 E.I. Du Pont De Nemours And Company Recombinant bacteria comprising novel sucrose transporters
US8686114B2 (en) * 2012-03-05 2014-04-01 E I Du Pont De Nemours And Company Variant sucrose transporter polypeptides

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US5686276A (en) 1995-05-12 1997-11-11 E. I. Du Pont De Nemours And Company Bioconversion of a fermentable carbon source to 1,3-propanediol by a single microorganism
JP4330041B2 (ja) * 1996-11-13 2009-09-09 イー・アイ・デュポン・ドウ・ヌムール・アンド・カンパニー 組換え生物体によるグリセロールの製造方法
JP4327909B2 (ja) * 1996-11-13 2009-09-09 イー・アイ・デユポン・ドウ・ヌムール・アンド・カンパニー 組み換え生物による1,3―プロパンジオールの生産方法
BR9815361B1 (pt) 1997-12-02 2010-10-05 método para produção de glicerol a partir de um microorganismo recombinante e microorganismo hospedeiro transformado.
CA2380616C (en) 1999-08-18 2011-05-24 E.I. Du Pont De Nemours And Company Process for the biological production of 1,3-propanediol with high titer
RU2212447C2 (ru) * 2000-04-26 2003-09-20 Закрытое акционерное общество "Научно-исследовательский институт Аджиномото-Генетика" Штамм escherichia coli - продуцент аминокислоты (варианты) и способ получения аминокислот (варианты)
CA2481414C (en) 2002-04-22 2013-04-09 E.I. Du Pont De Nemours And Company Promoter and plasmid system for genetic engineering
AU2003287028B2 (en) 2002-10-04 2008-09-04 E.I. Du Pont De Nemours And Company Process for the biological production of 1,3-propanediol with high yield
EP1546312B1 (en) * 2002-10-04 2014-07-30 Danisco US Inc. Glucose transport mutants for production of biomaterial
US7524660B2 (en) 2005-05-05 2009-04-28 E.I. Du Pont De Nemours And Company Utilization of fructose in microbial production strains
JP5546870B2 (ja) * 2007-03-23 2014-07-09 メタボリック エクスプローラー 1,2−プロパンジオールおよびアセトールの製造のための微生物および方法
ES2429305T3 (es) * 2008-11-07 2013-11-14 Metabolic Explorer Utilización de la sacarosa como sustrato para la producción fermentativa de 1,2-propanodiol

Also Published As

Publication number Publication date
AU2010325895B2 (en) 2014-11-06
SG181152A1 (en) 2012-07-30
JP2013512675A (ja) 2013-04-18
WO2011069033A1 (en) 2011-06-09
CN103080326A (zh) 2013-05-01
US20110136190A1 (en) 2011-06-09
EP2507377A1 (en) 2012-10-10
BR112012012719A2 (pt) 2015-09-29
AU2010325895A1 (en) 2012-05-31
KR20120099116A (ko) 2012-09-06

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Legal Events

Date Code Title Description
EEER Examination request

Effective date: 20150820

FZDE Discontinued

Effective date: 20171120