AU2010325895B2 - Recombinant bacteria for producing glycerol and glycerol-derived products from sucrose - Google Patents

Recombinant bacteria for producing glycerol and glycerol-derived products from sucrose Download PDF

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Publication number
AU2010325895B2
AU2010325895B2 AU2010325895A AU2010325895A AU2010325895B2 AU 2010325895 B2 AU2010325895 B2 AU 2010325895B2 AU 2010325895 A AU2010325895 A AU 2010325895A AU 2010325895 A AU2010325895 A AU 2010325895A AU 2010325895 B2 AU2010325895 B2 AU 2010325895B2
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ala
gly
val
ile
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AU2010325895A
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AU2010325895A1 (en
Inventor
Andrew C. Eliot
Anthony A. Gatenby
Tina K. Van Dyk
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EIDP Inc
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EI Du Pont de Nemours and Co
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2451—Glucanases acting on alpha-1,6-glucosidic bonds
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1205—Phosphotransferases with an alcohol group as acceptor (2.7.1), e.g. protein kinases
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/18—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic polyhydric
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/18—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic polyhydric
    • C12P7/20—Glycerol
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
    • C12P7/42—Hydroxy-carboxylic acids

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
AU2010325895A 2009-12-04 2010-12-03 Recombinant bacteria for producing glycerol and glycerol-derived products from sucrose Ceased AU2010325895B2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2014274643A AU2014274643B2 (en) 2009-12-04 2014-12-12 Recombinant bacteria for producing glycerol and glycerol-derived products from sucrose

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US26660509P 2009-12-04 2009-12-04
US61/266,605 2009-12-04
PCT/US2010/058832 WO2011069033A1 (en) 2009-12-04 2010-12-03 Recombinant bacteria for producing glycerol and glycerol-derived products from sucrose

Related Child Applications (1)

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AU2014274643A Division AU2014274643B2 (en) 2009-12-04 2014-12-12 Recombinant bacteria for producing glycerol and glycerol-derived products from sucrose

Publications (2)

Publication Number Publication Date
AU2010325895A1 AU2010325895A1 (en) 2012-05-31
AU2010325895B2 true AU2010325895B2 (en) 2014-11-06

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AU2010325895A Ceased AU2010325895B2 (en) 2009-12-04 2010-12-03 Recombinant bacteria for producing glycerol and glycerol-derived products from sucrose

Country Status (10)

Country Link
US (1) US20110136190A1 (enExample)
EP (1) EP2507377A1 (enExample)
JP (1) JP2013512675A (enExample)
KR (1) KR20120099116A (enExample)
CN (1) CN103080326A (enExample)
AU (1) AU2010325895B2 (enExample)
BR (1) BR112012012719A2 (enExample)
CA (1) CA2780757A1 (enExample)
SG (1) SG181152A1 (enExample)
WO (1) WO2011069033A1 (enExample)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8129170B1 (en) * 2010-12-06 2012-03-06 E.I. Du Pont De Nemours And Company Recombinant bacteria having the ability to metabolize sucrose
US8673602B2 (en) * 2011-08-16 2014-03-18 E I Du Pont De Nemours And Company Recombinant bacteria having improved sucrose utilization
US8629243B2 (en) 2011-08-16 2014-01-14 E I Du Pont De Nemours And Company Variant sucrose transporter polypeptides that enable faster sucrose utilization in bacteria
US9017961B2 (en) * 2012-03-05 2015-04-28 E.I. Du Pont De Nemours And Company Recombinant bacteria comprising novel sucrose transporters
US8686114B2 (en) * 2012-03-05 2014-04-01 E I Du Pont De Nemours And Company Variant sucrose transporter polypeptides

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6013494A (en) * 1996-11-13 2000-01-11 E. I. Du Pont De Nemours And Company Method for the production of 1,3-propanediol by recombinant microorganisms
EP1149911A2 (en) * 2000-04-26 2001-10-31 Ajinomoto Co., Ltd. Amino acid producing strains belonging to the genus Escherichia and method for producing amino acid

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US5686276A (en) 1995-05-12 1997-11-11 E. I. Du Pont De Nemours And Company Bioconversion of a fermentable carbon source to 1,3-propanediol by a single microorganism
JP4330041B2 (ja) * 1996-11-13 2009-09-09 イー・アイ・デュポン・ドウ・ヌムール・アンド・カンパニー 組換え生物体によるグリセロールの製造方法
BR9815361B1 (pt) 1997-12-02 2010-10-05 método para produção de glicerol a partir de um microorganismo recombinante e microorganismo hospedeiro transformado.
CA2380616C (en) 1999-08-18 2011-05-24 E.I. Du Pont De Nemours And Company Process for the biological production of 1,3-propanediol with high titer
CA2481414C (en) 2002-04-22 2013-04-09 E.I. Du Pont De Nemours And Company Promoter and plasmid system for genetic engineering
AU2003287028B2 (en) 2002-10-04 2008-09-04 E.I. Du Pont De Nemours And Company Process for the biological production of 1,3-propanediol with high yield
EP1546312B1 (en) * 2002-10-04 2014-07-30 Danisco US Inc. Glucose transport mutants for production of biomaterial
US7524660B2 (en) 2005-05-05 2009-04-28 E.I. Du Pont De Nemours And Company Utilization of fructose in microbial production strains
JP5546870B2 (ja) * 2007-03-23 2014-07-09 メタボリック エクスプローラー １，２−プロパンジオールおよびアセトールの製造のための微生物および方法
ES2429305T3 (es) * 2008-11-07 2013-11-14 Metabolic Explorer Utilización de la sacarosa como sustrato para la producción fermentativa de 1,2-propanodiol

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6013494A (en) * 1996-11-13 2000-01-11 E. I. Du Pont De Nemours And Company Method for the production of 1,3-propanediol by recombinant microorganisms
EP1149911A2 (en) * 2000-04-26 2001-10-31 Ajinomoto Co., Ltd. Amino acid producing strains belonging to the genus Escherichia and method for producing amino acid

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
D3 : JAHERIS ET AL: "Adaption of sucrose metabolism in the Escherichia coli wild-type strain EC3132". Journal of Bacteriology, 2002, vol. 184, no. 19, pages 5307-5316 *
D4 : CHOTANI ET AL: "The commercial production of chemicals using pathway engineering". Biochimica et Biophysica Acta, 2000, vol. 1543, no. 2, pages 434-455 *
D5 EDGAR ETAL Biosynthesis in E. coli of sn-glycerol 3-phosphate a precursor of phospholipid. Kinetic characterization of wild type & feedback-resistant forms of biosynthetic snglycerol- 3phosphate dehydrogenase JBC, 1978, v. 253,pg 6354-63 *
D6 : YEH ET AL: "Structure of glycerol-3-phosphate dehydrogenase, an essential monotopic membrane enzyme involved in repiration and metabolism". The Proceedings of the National Academy of Sciences, 2008, vol. 105, no. 9, pages 3280-3285 *

Also Published As

Publication number Publication date
SG181152A1 (en) 2012-07-30
JP2013512675A (ja) 2013-04-18
WO2011069033A1 (en) 2011-06-09
CN103080326A (zh) 2013-05-01
US20110136190A1 (en) 2011-06-09
EP2507377A1 (en) 2012-10-10
CA2780757A1 (en) 2011-06-09
BR112012012719A2 (pt) 2015-09-29
AU2010325895A1 (en) 2012-05-31
KR20120099116A (ko) 2012-09-06

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