CN103080321A - 具有提高外源基因表达活性的dna元件 - Google Patents

具有提高外源基因表达活性的dna元件 Download PDF

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Publication number
CN103080321A
CN103080321A CN2011800430998A CN201180043099A CN103080321A CN 103080321 A CN103080321 A CN 103080321A CN 2011800430998 A CN2011800430998 A CN 2011800430998A CN 201180043099 A CN201180043099 A CN 201180043099A CN 103080321 A CN103080321 A CN 103080321A
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CN
China
Prior art keywords
polynucleotide
sequence
seq
polynucleotide sequence
dna
Prior art date
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Pending
Application number
CN2011800430998A
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English (en)
Chinese (zh)
Inventor
西宫大祐
井上龙也
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Daiichi Sankyo Co Ltd
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Daiichi Sankyo Co Ltd
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Application filed by Daiichi Sankyo Co Ltd filed Critical Daiichi Sankyo Co Ltd
Priority to CN201610282337.5A priority Critical patent/CN105950621B/zh
Priority to CN201410141682.8A priority patent/CN103865931B/zh
Publication of CN103080321A publication Critical patent/CN103080321A/zh
Pending legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/10—Immunoglobulins specific features characterized by their source of isolation or production
    • C07K2317/14—Specific host cells or culture conditions, e.g. components, pH or temperature
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/20—Immunoglobulins specific features characterized by taxonomic origin
    • C07K2317/24—Immunoglobulins specific features characterized by taxonomic origin containing regions, domains or residues from different species, e.g. chimeric, humanized or veneered
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/51—Complete heavy chain or Fd fragment, i.e. VH + CH1
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/515—Complete light chain, i.e. VL + CL
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/10—Plasmid DNA
    • C12N2800/106—Plasmid DNA for vertebrates
    • C12N2800/107—Plasmid DNA for vertebrates for mammalian
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/46—Vector systems having a special element relevant for transcription elements influencing chromatin structure, e.g. scaffold/matrix attachment region, methylation free island

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  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Immunology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
CN2011800430998A 2010-07-07 2011-07-06 具有提高外源基因表达活性的dna元件 Pending CN103080321A (zh)

Priority Applications (2)

Application Number Priority Date Filing Date Title
CN201610282337.5A CN105950621B (zh) 2010-07-07 2011-07-06 具有提高外源基因表达活性的dna元件
CN201410141682.8A CN103865931B (zh) 2010-07-07 2011-07-06 具有提高外源基因表达活性的dna元件

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP2010154782 2010-07-07
JP2010-154782 2010-07-07
PCT/JP2011/065916 WO2012005378A2 (en) 2010-07-07 2011-07-06 Dna element having the activity of enhancing foreign gene expression

Related Child Applications (2)

Application Number Title Priority Date Filing Date
CN201610282337.5A Division CN105950621B (zh) 2010-07-07 2011-07-06 具有提高外源基因表达活性的dna元件
CN201410141682.8A Division CN103865931B (zh) 2010-07-07 2011-07-06 具有提高外源基因表达活性的dna元件

Publications (1)

Publication Number Publication Date
CN103080321A true CN103080321A (zh) 2013-05-01

Family

ID=44630215

Family Applications (3)

Application Number Title Priority Date Filing Date
CN2011800430998A Pending CN103080321A (zh) 2010-07-07 2011-07-06 具有提高外源基因表达活性的dna元件
CN201410141682.8A Active CN103865931B (zh) 2010-07-07 2011-07-06 具有提高外源基因表达活性的dna元件
CN201610282337.5A Active CN105950621B (zh) 2010-07-07 2011-07-06 具有提高外源基因表达活性的dna元件

Family Applications After (2)

Application Number Title Priority Date Filing Date
CN201410141682.8A Active CN103865931B (zh) 2010-07-07 2011-07-06 具有提高外源基因表达活性的dna元件
CN201610282337.5A Active CN105950621B (zh) 2010-07-07 2011-07-06 具有提高外源基因表达活性的dna元件

Country Status (14)

Country Link
US (3) US9371543B2 (cg-RX-API-DMAC7.html)
EP (3) EP2591113B1 (cg-RX-API-DMAC7.html)
JP (2) JP5859988B2 (cg-RX-API-DMAC7.html)
KR (3) KR101856927B1 (cg-RX-API-DMAC7.html)
CN (3) CN103080321A (cg-RX-API-DMAC7.html)
AU (3) AU2011274864B2 (cg-RX-API-DMAC7.html)
BR (1) BR112012033780A2 (cg-RX-API-DMAC7.html)
CA (2) CA2916937A1 (cg-RX-API-DMAC7.html)
ES (3) ES2565428T3 (cg-RX-API-DMAC7.html)
IL (1) IL243763A0 (cg-RX-API-DMAC7.html)
RU (1) RU2013104989A (cg-RX-API-DMAC7.html)
SG (4) SG10201705118RA (cg-RX-API-DMAC7.html)
TW (2) TW201604280A (cg-RX-API-DMAC7.html)
WO (1) WO2012005378A2 (cg-RX-API-DMAC7.html)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11555204B2 (en) 2016-10-03 2023-01-17 Daiichi Sankyo Company, Limited Promoter of Hspa5 gene
CN116555264A (zh) * 2018-08-14 2023-08-08 上海药明生物技术有限公司 转录调节元件及其在增强异源蛋白表达中的应用

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2591113B1 (en) * 2010-07-07 2016-01-20 Daiichi Sankyo Company, Limited Dna element having the activity of enhancing foreign gene expression
CN104066839B (zh) * 2011-11-28 2018-02-16 第一三共株式会社 衍生自人基因的启动子
CN107881174B (zh) * 2017-11-22 2022-06-03 张宝会 一种翻译水平基因表达调控的方法及应用
US20230192893A1 (en) * 2020-05-11 2023-06-22 Samsung Bioepis Co., Ltd. Human genome-derived polynucleotide and method for producing polypeptide of interest using same
WO2023210607A1 (ja) 2022-04-26 2023-11-02 第一三共株式会社 Ｅｎｏ１遺伝子のプロモーター

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1989007644A1 (en) * 1988-02-12 1989-08-24 Commonwealth Scientific And Industrial Research Or Pox virus vectors

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20070161003A1 (en) * 2003-09-29 2007-07-12 Morris David W Novel therapeutic targets in cancer
EP2105742A1 (en) * 2008-03-26 2009-09-30 Sanofi-Aventis Use of cathepsin C
JP5344682B2 (ja) * 2008-12-26 2013-11-20 グローブライド株式会社 釣糸ガイド、及び釣糸ガイドの製造方法
EP2591113B1 (en) * 2010-07-07 2016-01-20 Daiichi Sankyo Company, Limited Dna element having the activity of enhancing foreign gene expression
CN104066839B (zh) * 2011-11-28 2018-02-16 第一三共株式会社 衍生自人基因的启动子

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1989007644A1 (en) * 1988-02-12 1989-08-24 Commonwealth Scientific And Industrial Research Or Pox virus vectors

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
CORI GORMAN ET AL.: "Use of MAR Elements to Increase the Production of Recombinant Proteins", 《CELL LINE DEVELOPMENT》 *
WILSON R.: "EMI Database accession no. AC010724", 《EMI DATABASE》 *

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11555204B2 (en) 2016-10-03 2023-01-17 Daiichi Sankyo Company, Limited Promoter of Hspa5 gene
TWI812596B (zh) * 2016-10-03 2023-08-21 日商第一三共股份有限公司 Hspa5基因的啓動子
CN116555264A (zh) * 2018-08-14 2023-08-08 上海药明生物技术有限公司 转录调节元件及其在增强异源蛋白表达中的应用

Also Published As

Publication number Publication date
US20160333371A1 (en) 2016-11-17
US10407694B2 (en) 2019-09-10
CN103865931B (zh) 2018-10-02
KR101920187B1 (ko) 2018-11-19
US20130171694A1 (en) 2013-07-04
US11060108B2 (en) 2021-07-13
EP3026117B1 (en) 2018-09-12
KR101856927B1 (ko) 2018-05-10
JP2013531967A (ja) 2013-08-15
TW201604280A (zh) 2016-02-01
CA2916937A1 (en) 2012-01-12
CN105950621B (zh) 2019-07-30
SG10201705118RA (en) 2017-07-28
AU2018200686A1 (en) 2018-02-22
AU2011274864B2 (en) 2016-10-06
WO2012005378A3 (en) 2012-04-12
CN103865931A (zh) 2014-06-18
CN105950621A (zh) 2016-09-21
JP2016054741A (ja) 2016-04-21
WO2012005378A2 (en) 2012-01-12
IL243763A0 (en) 2016-04-21
JP5859988B2 (ja) 2016-02-16
KR20180021246A (ko) 2018-02-28
US20190345515A1 (en) 2019-11-14
AU2011274864A1 (en) 2013-02-07
ES2565428T3 (es) 2016-04-04
CA2804381A1 (en) 2012-01-12
TWI507523B (zh) 2015-11-11
SG10201505354PA (en) 2015-08-28
EP2684961A1 (en) 2014-01-15
KR20130111514A (ko) 2013-10-10
JP6039048B2 (ja) 2016-12-07
KR20180021247A (ko) 2018-02-28
EP3026117A1 (en) 2016-06-01
CA2804381C (en) 2016-03-22
AU2018200686B2 (en) 2020-02-13
KR101833570B1 (ko) 2018-03-02
BR112012033780A2 (pt) 2016-05-31
TW201207103A (en) 2012-02-16
EP2684961B1 (en) 2016-01-20
AU2016203681A1 (en) 2016-06-23
SG186788A1 (en) 2013-02-28
ES2564227T3 (es) 2016-03-21
EP2591113B1 (en) 2016-01-20
ES2701091T3 (es) 2019-02-20
SG2014009187A (en) 2014-04-28
US9371543B2 (en) 2016-06-21
AU2016203681B2 (en) 2018-04-19
EP2591113A2 (en) 2013-05-15
RU2013104989A (ru) 2014-08-20

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Application publication date: 20130501