CN103076422B - Ion chromatograph for multichannel detection and usage method thereof - Google Patents
Ion chromatograph for multichannel detection and usage method thereof Download PDFInfo
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- CN103076422B CN103076422B CN201310010399.7A CN201310010399A CN103076422B CN 103076422 B CN103076422 B CN 103076422B CN 201310010399 A CN201310010399 A CN 201310010399A CN 103076422 B CN103076422 B CN 103076422B
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- 238000001514 detection method Methods 0.000 title claims abstract description 48
- 238000000034 method Methods 0.000 title claims abstract description 20
- 238000005070 sampling Methods 0.000 claims abstract description 120
- 150000002500 ions Chemical class 0.000 claims abstract description 118
- 210000002700 urine Anatomy 0.000 claims abstract description 96
- 239000008280 blood Substances 0.000 claims abstract description 89
- 210000004369 blood Anatomy 0.000 claims abstract description 89
- MUBZPKHOEPUJKR-UHFFFAOYSA-N Oxalic acid Chemical compound OC(=O)C(O)=O MUBZPKHOEPUJKR-UHFFFAOYSA-N 0.000 claims abstract description 33
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 claims abstract description 33
- 238000002211 ultraviolet spectrum Methods 0.000 claims abstract description 19
- JLVVSXFLKOJNIY-UHFFFAOYSA-N Magnesium ion Chemical compound [Mg+2] JLVVSXFLKOJNIY-UHFFFAOYSA-N 0.000 claims abstract description 17
- LEHOTFFKMJEONL-UHFFFAOYSA-N Uric Acid Chemical compound N1C(=O)NC(=O)C2=C1NC(=O)N2 LEHOTFFKMJEONL-UHFFFAOYSA-N 0.000 claims abstract description 17
- TVWHNULVHGKJHS-UHFFFAOYSA-N Uric acid Natural products N1C(=O)NC(=O)C2NC(=O)NC21 TVWHNULVHGKJHS-UHFFFAOYSA-N 0.000 claims abstract description 17
- 229910001425 magnesium ion Inorganic materials 0.000 claims abstract description 17
- 229940116269 uric acid Drugs 0.000 claims abstract description 17
- 239000007788 liquid Substances 0.000 claims abstract description 14
- 229910001424 calcium ion Inorganic materials 0.000 claims abstract description 13
- 235000006408 oxalic acid Nutrition 0.000 claims abstract description 11
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 claims abstract description 9
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 claims abstract description 5
- 239000011575 calcium Substances 0.000 claims abstract description 5
- 238000004587 chromatography analysis Methods 0.000 claims description 58
- 238000002347 injection Methods 0.000 claims description 49
- 239000007924 injection Substances 0.000 claims description 49
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 claims description 32
- 238000002203 pretreatment Methods 0.000 claims description 24
- 229910000147 aluminium phosphate Inorganic materials 0.000 claims description 16
- 238000004140 cleaning Methods 0.000 claims description 16
- 229910019142 PO4 Inorganic materials 0.000 claims description 14
- YZCKVEUIGOORGS-IGMARMGPSA-N Protium Chemical compound [1H] YZCKVEUIGOORGS-IGMARMGPSA-N 0.000 claims description 14
- NBIIXXVUZAFLBC-UHFFFAOYSA-M dihydrogenphosphate Chemical compound OP(O)([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-M 0.000 claims description 14
- 239000010452 phosphate Substances 0.000 claims description 14
- -1 phosphate anion Chemical class 0.000 claims description 14
- BHPQYMZQTOCNFJ-UHFFFAOYSA-N Calcium cation Chemical compound [Ca+2] BHPQYMZQTOCNFJ-UHFFFAOYSA-N 0.000 claims description 12
- 229910052791 calcium Inorganic materials 0.000 claims description 4
- 239000012530 fluid Substances 0.000 claims description 4
- 230000001681 protective effect Effects 0.000 claims description 4
- 239000000356 contaminant Substances 0.000 claims description 3
- 230000033001 locomotion Effects 0.000 claims 1
- 239000011574 phosphorus Substances 0.000 abstract description 4
- 229910052698 phosphorus Inorganic materials 0.000 abstract description 4
- 230000002485 urinary effect Effects 0.000 abstract 2
- 230000000694 effects Effects 0.000 description 3
- 208000015924 Lithiasis Diseases 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 125000005587 carbonate group Chemical group 0.000 description 2
- 230000001276 controlling effect Effects 0.000 description 2
- 230000007547 defect Effects 0.000 description 2
- 150000002431 hydrogen Chemical class 0.000 description 2
- 229910052739 hydrogen Inorganic materials 0.000 description 2
- 239000001257 hydrogen Substances 0.000 description 2
- 238000004255 ion exchange chromatography Methods 0.000 description 2
- 238000002386 leaching Methods 0.000 description 2
- 230000001105 regulatory effect Effects 0.000 description 2
- 238000012163 sequencing technique Methods 0.000 description 2
- 230000001684 chronic effect Effects 0.000 description 1
- 238000004440 column chromatography Methods 0.000 description 1
- 238000004132 cross linking Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000007865 diluting Methods 0.000 description 1
- 238000004128 high performance liquid chromatography Methods 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 239000011347 resin Substances 0.000 description 1
- 229920005989 resin Polymers 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000012085 test solution Substances 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
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- Investigating Or Analysing Biological Materials (AREA)
Abstract
The invention relates to an ion chromatograph for multichannel detection. The ion chromatograph comprises an ion chromatograph body and an automatic sampler. The ion chromatograph body comprises a plurality of channels, and the automatic sampler performs automatic sampling to the plurality of the channels. A usage method of the ion chromatograph for multichannel detection comprises steps that: (1) urinary oxalic acid and urinary citric acid in urine samples are detected in a first ion chromatograph channel; (2) blood phosphorus in blood samples is detected in a second ion chromatograph channel; (3) calcium and magnesium ions in the blood samples and the urine samples are detected in a third ion chromatograph channel; and (4) uric acid in the blood samples and the urine samples is detected in ultraviolet spectrum detection channels. The ion chromatograph for multichannel detection has the advantages that various relevant components of liquid samples are detected simultaneously, and contents of relevant components and elements of calculus are obtained through intelligent calculation, so that the whole detection time can be shortened.
Description
Technical field
The present invention relates to a kind of ion chromatograph, particularly relating to one can detect indices data in plurality of liquid by hyperchannel simultaneously, shortens ion chromatograph and the using method thereof of detection time.
Background technology
Chromatography of ions is the one of high performance liquid chromatography, also known as high-efficient ion chromatogram (HPIC) or Modern Ion Chromatography, its difference being different from conventional ion exchange chromatography column chromatography mainly utilizes resin to have very high degree of crosslinking and lower exchange capacity, sampling volume is very little, usually on-line automatic continuous Conductivity detection is carried out to pouring fluid with ram pump conveying leacheate, thus the ion component in test solution and concentration can be detected.
Ion chromatograph of the prior art also also exists defect, such as in the application of reality, all need to detect to the every data target of sufferer, when namely needing to measure different kinds of ions composition in plurality of liquid and concentration, such as need to detect the urine oxalic acid in urine specimen and the calcium ion in serium inorganic phosphorus, blood sample and the urine specimen in urine citric acid, blood sample and the uric acid etc. in magnesium ion, blood sample and urine specimen simultaneously, traditional method can only by tested liquid successively sample introduction in ion chromatograph, what need is chronic, and efficiency is not high.So for this defect in ion chromatograph, the ion chromatograph be badly in need of now after a kind of improvement satisfies the demands.
Summary of the invention
The present invention is exactly to solve the problem, and overcomes problem existing in prior art intermediate ion chromatograph, the invention provides one can chemically examine indices data in plurality of liquid by hyperchannel simultaneously, shortens the ion chromatograph of chemical examination time to satisfy the demands.
To achieve these goals, technical scheme of the present invention is as follows:
An ion chromatograph for multi-channel detection, comprises ion chromatograph and automatic sampler, it is characterized in that, described ion chromatograph comprises the first sampling pump, oxalic acid is urinated for detecting in urine specimen, first chromatography of ions passage of urine citric acid, second sampling pump, for detecting phosphate anion in blood sample, second chromatography of ions passage of phosphoric acid one hydrogen radical ion and dihydrogen phosphate ions concentration, 3rd sampling pump, for detecting the 3rd chromatography of ions passage of calcium in urine specimen and blood sample and magnesium ion, 4th sampling pump and the ultraviolet spectrum sense channel for detecting the uric acid in urine specimen and blood sample, described automatic sampler connects the first sampling pump respectively, second sampling pump, 3rd sampling pump and the 4th sampling pump, described first sampling pump connects the first chromatography of ions passage, second sampling pump connects the second chromatography of ions passage, 3rd sampling pump connects the 3rd chromatography of ions passage, and the 4th sampling pump connects ultraviolet spectrum sense channel, between described first sampling pump and the first chromatography of ions passage, between second sampling pump and the second chromatography of ions passage, between 3rd sampling pump and the 3rd chromatography of ions passage, the first sampling valve is respectively arranged with between 4th sampling pump and ultraviolet spectrum sense channel, second sampling valve, 3rd sampling valve, 4th sampling valve.
The ion chromatograph of above-mentioned a kind of multi-channel detection; it is characterized in that, be provided with for the protection of chromatographic column, protective device sample being carried out to contaminant filter between the first sampling valve and the first chromatography of ions passage, between the second sampling valve and the second chromatography of ions passage, between the 3rd sampling valve and the 3rd chromatography of ions passage, between the 4th sampling valve and ultraviolet spectrum sense channel.
The ion chromatograph of above-mentioned a kind of multi-channel detection, is characterized in that, described automatic sampler comprises electronic machine mechanical arm, sample introduction needle and the sample injection disc that three direction of principal axis move, and described sample introduction needle is connected on mechanical arm, and described sample injection disc is arranged at below sample introduction needle.
The ion chromatograph of above-mentioned a kind of multi-channel detection, is characterized in that, described sample introduction needle is micro-sampling pin.
The ion chromatograph of above-mentioned a kind of multi-channel detection, is characterized in that, also comprise the cleaning device for cleaning sample introduction needle, described cleaning device comprises housing, housing is provided with the rinse bath cleaned sample introduction needle for splendid attire cleaning fluid.
The ion chromatograph of above-mentioned a kind of multi-channel detection, is characterized in that, described sample injection disc comprises base and the sample injection bottle for splendid attire sample to be tested, and described base is provided with a plurality of pickup groove for placing sample injection bottle, described sample injection bottle is arranged in pickup groove.
The ion chromatograph of above-mentioned a kind of multi-channel detection, is characterized in that, described sample injection disc is square, and described pickup groove is that geometry is arranged on square sample injection disc.
The ion chromatograph of above-mentioned a kind of multi-channel detection, it is characterized in that, described urine specimen be have passed through pre-treatment urine specimen and without one of them of the urine specimen of pre-treatment, described blood sample be have passed through pre-treatment blood sample and without one of them of the blood sample of pre-treatment.
A using method for the ion chromatograph of multi-channel detection, is characterized in that, comprises the following steps:
(1) mechanical arm drives sample introduction needle to extract urine specimen in sample injection bottle, urine specimen is injected in the first sampling pump, and urine specimen is delivered in the first chromatography of ions passage the urine oxalic acid detected in urine specimen, urine citric acid by the first sampling pump;
(2) mechanical arm drives sample introduction needle to extract blood sample in sample injection bottle, blood sample is injected in the second sampling pump, blood sample is delivered in the second chromatography of ions passage by the second sampling pump, detect one of them of the concentration of phosphoric acid one hydrogen radical ion, phosphate anion and dihydrogen phosphate ions in blood sample, be then converted into the content of element phosphor in blood by the molecular formula of known phosphoric acid one hydrogen radical ion, phosphate anion and dihydrogen phosphate ions and molecular weight.Here it is to be noted that, we can adjust blood sample by the pH value controlling ion chromatogram eluate is exist in which form after entering chromatography of ions passage, thus determine again to carry out content detection to the element of its existence form, the content of element phosphor in blood is converted into finally by the molecular formula of this known element and molecular weight.In one of them optimal way of the present invention, the ion chromatogram eluate that we use is carbonate system, its pH value is stablized and can be regulated, after we can make detection sample enter chromatography of ions passage by the pH value of Non-leaching liquid, phosphorus contained by it mainly exists with the form of phosphoric acid one hydrogen root, detects only go out a peak at chromatography of ions;
(3) mechanical arm drives sample introduction needle to extract urine specimen in sample injection bottle, urine specimen is injected in the 3rd sampling pump, urine specimen is delivered in the 3rd chromatography of ions passage and detects calcium ion in urine specimen and magnesium ion by the 3rd sampling pump, after calcium ion in urine specimen to be detected and magnesium ion terminate, then mechanical arm drives sample introduction needle to extract blood sample in sample injection bottle, be injected into by blood sample in the 3rd sampling pump, blood sample is delivered in the 3rd chromatography of ions passage and detects calcium ion in blood sample and magnesium ion by the 3rd sampling pump;
(4) mechanical arm drives sample introduction needle to extract urine specimen in sample injection bottle, urine specimen is injected in the 4th sampling pump, urine specimen is delivered in ultraviolet spectrum sense channel the uric acid detected in urine specimen by the 4th sampling pump, after uric acid in urine specimen to be detected terminates, then mechanical arm drives sample introduction needle to extract blood sample in sample injection bottle, be injected into by blood sample in the 4th sampling pump, blood sample is delivered in ultraviolet spectrum sense channel the uric acid detected in blood sample by the 4th sampling pump.
The using method of the ion chromatograph of above-mentioned a kind of multi-channel detection, it is characterized in that, in described (2) step, blood sample is delivered in the second chromatography of ions passage by the second sampling pump, detects wherein more than any one of the concentration of phosphoric acid one hydrogen radical ion, phosphate anion and dihydrogen phosphate ions in blood sample.
The using method of the ion chromatograph of above-mentioned a kind of multi-channel detection, is characterized in that, described sample introduction needle each sample introduction 3-10 microlitre.
The using method of the ion chromatograph of above-mentioned a kind of multi-channel detection, is characterized in that, after the complete detected liquid of described sample introduction needle per injection, mechanical arm drives in sample introduction needle to cleaning device and cleans.
The using method of the ion chromatograph of above-mentioned a kind of multi-channel detection, is characterized in that, the order of described step (1) to (4) can be upset and carries out.
The using method of the ion chromatograph of above-mentioned a kind of multi-channel detection, it is characterized in that, described urine specimen be have passed through pre-treatment urine specimen and without one of them of the urine specimen of pre-treatment, described blood sample be have passed through pre-treatment blood sample and without one of them of the blood sample of pre-treatment.
The invention has the beneficial effects as follows: owing to detecting the multiple Related Component of plurality of liquid sample simultaneously, and pass through Intelligent Calculation, provide the content of calculus Related Component or element, so the time of whole detection can be shortened, enhance the work efficiency of ion chromatograph greatly, simplify clinical workflow, also simultaneously for the control patient of lithiasis patient has striven for the valuable time.
Accompanying drawing explanation
Fig. 1 is structured flowchart of the present invention.
Embodiment
The technological means realized to make the present invention, creation characteristic, reaching object and effect is easy to understand, below in conjunction with concrete diagram, setting forth the present invention further.
Referring to Fig. 1, a kind of ion chromatograph of multi-channel detection, comprises ion chromatograph 100 and automatic sampler 200, by automatic sampler 200 sample introduction, is then transported in ion chromatograph 100 by detection sample and detects.
Ion chromatograph 100 comprises the first sampling pump 111, oxalic acid is urinated for detecting in urine specimen, first chromatography of ions passage 112 of urine citric acid, second sampling pump 121, for detecting phosphate anion in blood sample, second chromatography of ions passage 122 of phosphoric acid one hydrogen radical ion and dihydrogen phosphate ions concentration, 3rd sampling pump 131, for detecting the 3rd chromatography of ions passage 132 of calcium in urine specimen and blood sample and magnesium ion, 4th sampling pump 141 and the ultraviolet spectrum sense channel 142 for detecting the uric acid in urine specimen and blood sample, automatic sampler 200 connects the first sampling pump 111 respectively, second sampling pump 121, 3rd sampling pump 131 and the 4th sampling pump 141, first sampling pump 111 connects the first chromatography of ions passage 112, second sampling pump 121 connects the second chromatography of ions passage 122, 3rd sampling pump 131 connects the 3rd chromatography of ions passage 132, 4th sampling pump 141 connects ultraviolet spectrum sense channel 142.Carry out sample introduction by automatic sampler 200 when work, and then be transported to respectively in each passage in ion chromatograph 100 and detect respectively.In the process detected, detect multiple object time overall length depend on detection time of the project wherein needing detection time the longest, i.e. short-board effect, and in the operation of reality, detect in urine specimen and urinate oxalic acid, the time of urine citric acid is approximately 30 minutes, detect phosphate anion in blood sample, the time of phosphoric acid one hydrogen radical ion and dihydrogen phosphate ions concentration is approximately 10-15 minute, calcium ion in detection urine specimen and the time of magnesium ion are approximately 10 minutes, calcium ion in detection blood sample and the time of magnesium ion are approximately 10 minutes, the time detecting the uric acid in urine specimen is approximately 10 minutes, the time detecting the uric acid in blood sample is approximately 10 minutes, therefore comprehensive above various detection time, the urine oxalic acid that the present invention will need spended time the longest, urine citric acid arranges separately a passage and detects, by phosphate anion in blood sample, the detection of phosphoric acid one hydrogen radical ion and dihydrogen phosphate ions concentration arranges separately a passage and detects, calcium in urine specimen and blood sample and magnesium ion are put into a passage according to sequencing detect, uric acid in urine specimen and blood sample is put into a passage according to sequencing detect, such four passages work simultaneously, except detecting urine oxalic acid, the passage of urine citric acid needs outside cost 30 minutes, the detection time of other passage is all within 30 minutes, that is, whole testing all completed in 30 minutes.
Be respectively arranged with the first sampling valve 113, second sampling valve 123, the 3rd sampling valve 133, the 4th sampling valve 143, first sampling valve 113, second sampling valve 123, the 3rd sampling valve 133 and the 4th sampling valve 143 between first sampling pump 111 and the first chromatography of ions passage 112, between the second sampling pump 121 and the second chromatography of ions passage 122, between the 3rd sampling pump 131 and the 3rd chromatography of ions passage 132, between the 4th sampling pump 141 and ultraviolet spectrum sense channel 142 and control opening and closedown of each passage.
Between the first sampling valve 113 and the first chromatography of ions passage 112, between the second sampling valve 123 and the second chromatography of ions passage 122, be provided with for the protection of chromatographic column, the protective device 150 sample being carried out to contaminant filter between the 3rd sampling valve 133 and the 3rd chromatography of ions passage 132, between the 4th sampling valve 143 and ultraviolet spectrum sense channel 142.In wherein a kind of optimal way of the present invention, protective device 150 is guard column.
In the present invention, urine specimen to be detected be have passed through pre-treatment urine specimen and without one of them of the urine specimen of pre-treatment, blood sample also for have passed through pre-treatment blood sample and without one of them of the blood sample of pre-treatment.
Automatic sampler 200 comprises electronic machine mechanical arm 210, sample introduction needle 220 and the sample injection disc 230 that three direction of principal axis move, and sample introduction needle 220 is connected on mechanical arm 210, and sample injection disc 230 is arranged at below sample introduction needle 220.When work, mechanical arm 210 drives sample introduction needle 220 can along X, Y, Z three-axis moving, and sample injection disc 230 is arranged at the below of sample introduction needle 220, samples so sample introduction needle 220 can stretch in sample injection disc 230 under the drive of mechanical arm 210.
In traditional sample injection method; be not corroded to protect the parts in sense channel; the method taked is all need first to dilute detected sample; and then by being injected in sense channel manually; so not only unusual trouble; inefficiency, also add the workload of operator simultaneously.And in the present invention, sample introduction needle 220 is micro-sampling pin, each sample introduction content is only 3-10 microlitre, completely can when not diluting sample, directly be delivered in sense channel by sample introduction needle, do not need operator manually to inject simultaneously, reach the effect of full-automatic sample introduction.
Owing to extract a sample injection sense channel in each sample introduction needle 220 after, the residual liquid of last sample also may be remained in sample introduction needle 220, so the present invention also comprises the cleaning device 240 for cleaning sample introduction needle 220, cleaning device 240 comprises housing, housing is provided with rinse bath sample introduction needle cleaned for splendid attire cleaning fluid, after each sample introduction needle 220 sample drawn sample introduction, drive by mechanical arm 210 in the rinse bath of sample introduction needle 220 to cleaning device 240 and once clean, wash the residual liquid in most sample introduction needle 220.
Sample injection disc 230 comprises base and the sample injection bottle for splendid attire sample to be tested, and base is provided with a plurality of pickup groove for placing sample injection bottle, sample injection bottle is arranged in pickup groove.And in one of them preferred implementation of the present invention, sample injection disc 230 is square, pickup groove is that geometry is arranged on square sample injection disc 230.
A using method for the ion chromatograph of multi-channel detection, is characterized in that, comprises the following steps:
(1) mechanical arm 210 drives sample introduction needle 220 to extract urine specimen in sample injection bottle, urine specimen be injected in the first sampling pump 111, urine specimen is delivered in the first chromatography of ions passage 112 the urine oxalic acid detected in urine specimen, urine citric acid by the first sampling pump 111;
(2) mechanical arm 210 drives sample introduction needle 220 to extract blood sample in sample injection bottle, blood sample is injected in the second sampling pump 121, blood sample is delivered in the second chromatography of ions passage 122 by the second sampling pump 121, detect one of them of the concentration of phosphoric acid one hydrogen radical ion, phosphate anion and dihydrogen phosphate ions in blood sample, be then converted into the content of element phosphor in blood by the molecular formula of known phosphoric acid one hydrogen radical ion, phosphate anion and dihydrogen phosphate ions and molecular weight.Here it is to be noted that, we can adjust blood sample by the pH value controlling ion chromatogram eluate is exist in which form after entering chromatography of ions passage, thus determine again to carry out content detection to the element of its existence form, the content of element phosphor in blood is converted into finally by the molecular formula of this known element and molecular weight.In one of them optimal way of the present invention, the ion chromatogram eluate that we use is carbonate system, its pH value is stablized and can be regulated, after we can make detection sample enter chromatography of ions passage by the pH value of Non-leaching liquid, phosphorus contained by it mainly exists with the form of phosphoric acid one hydrogen root, detects only go out a peak at chromatography of ions;
(3) mechanical arm 210 drives sample introduction needle 220 to extract urine specimen in sample injection bottle, urine specimen is injected in the 3rd sampling pump 131, urine specimen is delivered in the 3rd chromatography of ions passage 132 and detects calcium ion in urine specimen and magnesium ion by the 3rd sampling pump 131, after calcium ion in urine specimen to be detected and magnesium ion terminate, then mechanical arm 210 drives sample introduction needle 220 to extract blood sample in sample injection bottle, blood sample is injected in the 3rd sampling pump 131, blood sample is delivered in the 3rd chromatography of ions passage 132 and detects calcium ion in blood sample and magnesium ion by the 3rd sampling pump 131,
(4) mechanical arm 210 drives sample introduction needle 220 to extract urine specimen in sample injection bottle, urine specimen is injected in the 4th sampling pump 141, urine specimen is delivered in ultraviolet spectrum sense channel 142 uric acid detected in urine specimen by the 4th sampling pump 141, after uric acid in urine specimen to be detected terminates, then mechanical arm 210 drives sample introduction needle 220 to extract blood sample in sample injection bottle, be injected into by blood sample in the 4th sampling pump 141, blood sample is delivered in ultraviolet spectrum sense channel 142 uric acid detected in blood sample by the 4th sampling pump 141.
In the present invention, urine specimen to be detected be have passed through pre-treatment urine specimen and without one of them of the urine specimen of pre-treatment, blood sample also for have passed through pre-treatment blood sample and without one of them of the blood sample of pre-treatment.
In (2) step, blood sample is delivered in the second chromatography of ions passage 122 by the second sampling pump 121, detects wherein more than any one of the concentration of phosphoric acid one hydrogen radical ion, phosphate anion and dihydrogen phosphate ions in blood sample.
In order to protect the parts in sense channel not to be corroded, each sample introduction 3-10 microlitre of sample introduction needle 220.After the complete detected liquid of sample introduction needle 220 per injection simultaneously, mechanical arm 210 drives in sample introduction needle 220 to cleaning device 240 and cleans.
In the present invention, the order of step (1) to (4) can be upset and carries out.
The invention has the beneficial effects as follows: owing to detecting the multiple Related Component of plurality of liquid sample simultaneously, and pass through Intelligent Calculation, provide the content of calculus Related Component or element, so the time of whole detection can be shortened, enhance the work efficiency of ion chromatograph greatly, simplify clinical workflow, also simultaneously for the control patient of lithiasis patient has striven for the valuable time.
More than show and describe ultimate principle of the present invention, principal character and advantage of the present invention.The technician of the industry should understand; the present invention is not restricted to the described embodiments; what describe in above-described embodiment and instructions just illustrates principle of the present invention; the present invention also has various changes and modifications without departing from the spirit and scope of the present invention, and these changes and improvements all fall in the claimed scope of the invention.Application claims protection domain is defined by appending claims and equivalent thereof.
Claims (9)
1. an ion chromatograph for multi-channel detection, comprises ion chromatograph and automatic sampler, it is characterized in that, described ion chromatograph comprises the first sampling pump, oxalic acid is urinated for detecting in urine specimen, first chromatography of ions passage of urine citric acid, second sampling pump, for detecting phosphate anion in blood sample, second chromatography of ions passage of phosphoric acid one hydrogen radical ion and dihydrogen phosphate ions concentration, 3rd sampling pump, for detecting the 3rd chromatography of ions passage of calcium in urine specimen and blood sample and magnesium ion, 4th sampling pump and the ultraviolet spectrum sense channel for detecting the uric acid in urine specimen and blood sample, described automatic sampler connects the first sampling pump respectively, second sampling pump, 3rd sampling pump and the 4th sampling pump, described first sampling pump connects the first chromatography of ions passage, second sampling pump connects the second chromatography of ions passage, 3rd sampling pump connects the 3rd chromatography of ions passage, and the 4th sampling pump connects ultraviolet spectrum sense channel, between described first sampling pump and the first chromatography of ions passage, between second sampling pump and the second chromatography of ions passage, between 3rd sampling pump and the 3rd chromatography of ions passage, the first sampling valve is respectively arranged with between 4th sampling pump and ultraviolet spectrum sense channel, second sampling valve, 3rd sampling valve, 4th sampling valve, described automatic sampler comprises the electronic machine mechanical arm of three direction of principal axis motions, sample introduction needle and sample injection disc, described sample introduction needle is connected on mechanical arm, described sample injection disc is arranged at below sample introduction needle, described sample introduction needle is micro-sampling pin, also comprise the cleaning device for cleaning sample introduction needle, described cleaning device comprises housing, housing is provided with the rinse bath cleaned sample introduction needle for splendid attire cleaning fluid.
2. the ion chromatograph of a kind of multi-channel detection according to claim 1; it is characterized in that, be provided with for the protection of chromatographic column, protective device sample being carried out to contaminant filter between the first sampling valve and the first chromatography of ions passage, between the second sampling valve and the second chromatography of ions passage, between the 3rd sampling valve and the 3rd chromatography of ions passage, between the 4th sampling valve and ultraviolet spectrum sense channel.
3. the ion chromatograph of a kind of multi-channel detection according to claim 2, it is characterized in that, described sample injection disc comprises base and the sample injection bottle for splendid attire sample to be tested, described base is provided with a plurality of pickup groove for placing sample injection bottle, described sample injection bottle is arranged in pickup groove, described sample injection disc is square, and described pickup groove is that geometry is arranged on square sample injection disc.
4. the ion chromatograph of a kind of multi-channel detection according to claim 1, it is characterized in that, described urine specimen be have passed through pre-treatment urine specimen and without one of them of the urine specimen of pre-treatment, described blood sample be have passed through pre-treatment blood sample and without one of them of the blood sample of pre-treatment.
5. a using method for the ion chromatograph of multi-channel detection, is characterized in that, comprises the following steps:
(1) mechanical arm drives sample introduction needle to extract urine specimen in sample injection bottle, urine specimen is injected in the first sampling pump, and urine specimen is delivered in the first chromatography of ions passage the urine oxalic acid detected in urine specimen, urine citric acid by the first sampling pump;
(2) mechanical arm drives sample introduction needle to extract blood sample in sample injection bottle, blood sample is injected in the second sampling pump, blood sample is delivered in the second chromatography of ions passage by the second sampling pump, detect one of them of the concentration of phosphoric acid one hydrogen radical ion, phosphate anion and dihydrogen phosphate ions in blood sample, be then converted into the content of element phosphor in blood by the molecular formula of known phosphoric acid one hydrogen radical ion, phosphate anion and dihydrogen phosphate ions and molecular weight;
(3) mechanical arm drives sample introduction needle to extract urine specimen in sample injection bottle, urine specimen is injected in the 3rd sampling pump, urine specimen is delivered in the 3rd chromatography of ions passage and detects calcium ion in urine specimen and magnesium ion by the 3rd sampling pump, after calcium ion in urine specimen to be detected and magnesium ion terminate, then mechanical arm drives sample introduction needle to extract blood sample in sample injection bottle, be injected into by blood sample in the 3rd sampling pump, blood sample is delivered in the 3rd chromatography of ions passage and detects calcium ion in blood sample and magnesium ion by the 3rd sampling pump;
(4) mechanical arm drives sample introduction needle to extract urine specimen in sample injection bottle, urine specimen is injected in the 4th sampling pump, urine specimen is delivered in ultraviolet spectrum sense channel the uric acid detected in urine specimen by the 4th sampling pump, after uric acid in urine specimen to be detected terminates, then mechanical arm drives sample introduction needle to extract blood sample in sample injection bottle, be injected into by blood sample in the 4th sampling pump, blood sample is delivered in ultraviolet spectrum sense channel the uric acid detected in blood sample by the 4th sampling pump.
6. the using method of a kind of ion chromatograph of multi-channel detection according to claim 5, is characterized in that, described in
step(2) in, blood sample is delivered in the second chromatography of ions passage by the second sampling pump, detects wherein more than any one of the concentration of phosphoric acid one hydrogen radical ion, phosphate anion and dihydrogen phosphate ions in blood sample.
7. the using method of a kind of ion chromatograph of multi-channel detection according to claim 6, it is characterized in that, described sample introduction needle each sample introduction 3-10 microlitre, after the complete detected liquid of described sample introduction needle per injection, mechanical arm drives in sample introduction needle to cleaning device and cleans.
8. the using method of a kind of ion chromatograph of multi-channel detection according to claim 5, is characterized in that, the order of described step (1) to (4) can be upset and carries out.
9. the using method of a kind of ion chromatograph of multi-channel detection according to claim 5, it is characterized in that, described urine specimen be have passed through pre-treatment urine specimen and without one of them of the urine specimen of pre-treatment, described blood sample be have passed through pre-treatment blood sample and without one of them of the blood sample of pre-treatment.
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CN201310010399.7A CN103076422B (en) | 2013-01-11 | 2013-01-11 | Ion chromatograph for multichannel detection and usage method thereof |
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Effective date of registration: 20161201 Address after: 430079 A7 Exhibition Center, No. 858, high tech Avenue, East Lake Development Zone, Hubei, Wuhan, China Patentee after: Five channel medical technology (Wuhan) Co., Ltd. Address before: 310013 Hangzhou, Zhejiang Province, Xihu District, Zhejiang Road, No. 38, No. Patentee before: Liu Guanlin |