CN103074253A - Composite flora and additive cellulose preparation method by using the same - Google Patents
Composite flora and additive cellulose preparation method by using the same Download PDFInfo
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- CN103074253A CN103074253A CN2012103425555A CN201210342555A CN103074253A CN 103074253 A CN103074253 A CN 103074253A CN 2012103425555 A CN2012103425555 A CN 2012103425555A CN 201210342555 A CN201210342555 A CN 201210342555A CN 103074253 A CN103074253 A CN 103074253A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
- Y02A40/20—Fertilizers of biological origin, e.g. guano or fertilizers made from animal corpses
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/30—Fuel from waste, e.g. synthetic alcohol or diesel
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
Abstract
The present invention relates to a composite flora and an additive cellulose preparation method by using the composite flora. The composite flora comprises rheinheimera tangshanensis with a preservation number of CGMCC No.5972, acinetobacter lwoffii with a preservation number of CGMCC No.5973, pseudomonas fluorescens with a preservation number of CGMCC No.5974, and Wickerhamomyces anomalus with a preservation number of CGMCC No.5975. The method comprises the following steps: preparing a bacterial liquid; treating raw materials; and preparing fibers, wherein fiber preparation comprises fiber kneading or squeezing rolling, biodegradation, steam sterilization, fiber obtaining, sterilization and grinding. According to the present invention, the method does not pollute the environment, a waste liquid is directly converted into an organic fertilizer so as to achieve zero emission and zero pollution, the biological treatment process provides a protection effect for fibers, advantages of low production cost and high economic benefits are provided compared with the traditional chemical method, and characteristics of energy source saving and environmental protection are provided.
Description
Technical field
The present invention relates to a kind of additive Mierocrystalline cellulose, relate in particular to a kind of composite flora and prepare the cellulosic method of additive with it.
Background technology
Mierocrystalline cellulose is compound of polysaccharide, is the natural reproducible resource of human beings preciousness.Before cellulose chemistry and industry start from more than 160 year, polymer chemistry birth and the main study subject of developing period, cellulosic major physiological effect is the absorption large quantity of moisture, increase the ight soil amount, promote intestinal peristalsis, accelerate the drainage of ight soil, the residence time of carcinogenic substance in enteron aisle shortened, pessimal stimulation to enteron aisle reduces, thereby can the prevention of intestinal oncogenesis.Prepare Mierocrystalline cellulose in the prior art, how by chemical process, the waste liquor contamination sublimity that produces during chemical process is produced, damage to land, polluted air, and also power consumption is high, and power consumption and water consumption are large.Do not meet national energy-saving and reduce discharging policy.Material can not reach the Efficient Cycle recycling.Chemicals can't separate from waste liquid, and organism and chemicals mix, and organism also can't be reused, and causes a large amount of losses.
Therefore, be necessary to develop biological cellulose technology processed, fundamentally solve an above-mentioned pollution difficult problem, energy-saving and emission-reduction, water-saving reduces production costs and improves the rate of utilization of material.
Summary of the invention
The objective of the invention is to overcome the cellulosic defects of chemical legal system of prior art, a kind of new preparation Mierocrystalline cellulose biological bacteria is provided.The contriver has obtained to be suitable for preparing the cellulosic biological bacteria that can realize the foregoing invention purpose by long screening operation.
Particularly, the invention provides a kind of composite flora, it comprises the silent Salmonella (Rheinheimera tangshanensis) of Tangshan Lay mattress that preserving number is CGMCC No.5972, preserving number is the acinetobacter lwoffii (Acinetobacter lwoffii) of CGMCC No.5973, preserving number is the Pseudomonas fluorescens (Pseudomonas fluoscens) of CGMCC No.5974, preserving number be this Wickerhamomyces anomalus bacterium of Wickerhamomyces anomalus(of CGMCC No.5975 in malt juice liquid medium 25 ℃ cultivated three days, cell is spherical, avette, sausage shape, size is (4.8-14.4) * (3.6-7.2) μ m, has precipitation to form.25 ℃ of cultivations in wort agar inclined-plane one month, bacterium colony cheese shape, oyster white, surface smoothing, not reflective, edge root shape.Corn Meal Agar Dalmau is dull and stereotyped to be cultivated, and produces pseudohypha).
Described composite flora can be used in preparation additive Mierocrystalline cellulose.
The present invention also provides a kind of cellulosic method of additive for preparing, and it mainly comprises step:
1) configuration of bacterium liquid: above-mentioned composite flora is configured to the composite bacteria aqueous solution according to following mass ratio, is bacterium liquid:
The Tangshan Lay mattress Salmonella of writing from memory: acinetobacter lwoffii: Pseudomonas fluorescens: Wickerhamomyces anomalus is 1-2:1-2:1-2:2-3;
2) raw material is processed: will cut into slices after the woody raw material peeling, or the draft raw material is cut into chunks, the raw material that cuts is put into the fermentation vat swollen;
3) preparation of fiber, it comprises step:
Discongest: with raw material thread rolling and/or crowded stone roller of swollen;
Biological degradation: the raw material soaking after will discongesting is in the bacterium liquid that configures;
Wet sterilization: with the raw material after the above-mentioned biological degradation from bacterium liquid, pull out, draining, pass into water vapor sterilization;
Obtaining of fiber: the raw material after will sterilizing carries out one section corase grind, becomes fibrous bundle; Above-mentioned one section corase grind is carried out two sections fine grindings, make fibrous bundle disperse into single fiber; Screening, filter through the fibrous bundle in the slurries after one section corase grind and the two sections fine grindings, defibrination makes it make single fiber again;
Sterilization: the above-mentioned fiber that makes is soaked in warm water, then dry, sterilize;
Grind: the fiber after will sterilizing grinds to form Mierocrystalline cellulose, as additive.
The density of the bacterium liquid that wherein, forms in the step 1) is 6 * 10
7Individual/more than the ml bacterium.
Wherein, step 2) in, the woody raw material length that cuts is 3-4cm, the draft raw material length that cuts is 4-5cm.The described swollen time is 10-12h.
Wherein, the described biological degradation temperature of step 3) remains on 35-40 ℃, time 30-34 hour.The mass ratio of the raw material after discongesting and bacterium liquid is 1:6-8.Described steam sterilizing is water at atmospheric pressure steam sterilizing 10-30 minute.
Further, the soak solution step 2) behind the immersion raw material is through flocculating, precipitate, supernatant liquor recycling, throw out input methane-generating pit fermentation generation biogas.
Biological method provided by the invention prepares cellulosic advantage: 1) free from environmental pollution: waste liquid directly changes into organic fertilizer, reaches zero release, and zero pollutes.2) biological method can play a protective role to fiber, compares with traditional chemical process, and present method can all reclaim full fiber and half fiber, has therefore improved yield.3) biological method carries out lignin degradation under normal pressure, energy-conservation, reduction of discharging, low-carbon (LC).4) production cost is low, and economic benefit is high.
Byproduct of the present invention is transported to settling tank flocculation, precipitation, and supernatant liquor returns second stage employ, remakes presoak and uses.Contain gas chromatography and the nutrient for plants such as N, P, K such as abundant xylogen in the throw out, throw out mixes with old bacterium liquid (the raw material thickness bacterium liquid of repeatedly degrading also contains N, P, K, Fe and trace element) again, acidifying, then together enter in the marsh gas fermentation pool, produce biogas.With the Boiler Ash mixing granulation of natural pond slag, natural pond liquid and pulverizing, the granulation fertilizer dispatches from the factory at last, realizes zero release.
The biological bacteria of the present invention by providing above-mentioned contriver to obtain through long creation work, the further perfect cellulosic technology of existing preparation, this technology reduced the reaction times, improved the purity and the yield that make fiber, so that can be applied in actual production on a large scale.Applying biological bacterium of the present invention at short notice in the degrading plant body xylogen obtain fiber, the byproduct twice transformation becomes biogas, biogas coal supply, gas boiler for dual purpose combustion heating are saved coal consumption.At last, the natural pond slag is made organic fertilizer, has formed the economic circulation new model of " material organic transformation ", reaches the no waste discharging, namely zero release.Fundamentally solved the pollution difficult problem of chemical preparation fiber of the prior art.Energy-saving and emission-reduction, water-saving has reduced production cost, has improved the rate of utilization of material.
For above and other objects of the present invention, feature and advantage can be become apparent, preferred embodiment cited below particularly, and cooperation accompanying drawing are described in detail below.
Description of drawings
Fig. 1 is the cellulosic schema of preparation additive according to one embodiment of the invention;
Fig. 2 is the cellulosic schema of preparation additive according to another embodiment of the present invention.
Embodiment
The configuration of embodiment 1 bacterium liquid
The biological bacteria that adopts among the present invention on April 6th, 2012 at the common micro-organisms center (CGMCC of China Committee for Culture Collection of Microorganisms, No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City) preservation, it comprises the silent Salmonella (Rheinheimera tangshanensis) of Tangshan Lay mattress that preserving number is CGMCC No.5972, preserving number is the acinetobacter lwoffii (Acinetobacter lwoffii) of CGMCC No.5973, preserving number is the Pseudomonas fluorescens (Pseudomonas fluorescens) of CGMCC No.5974, preserving number is the Wickerhamomyces anomalus of CGMCC No.5975.
Above-mentioned composite flora is configured to the composite bacteria aqueous solution according to following mass ratio, is bacterium liquid:
The Tangshan Lay mattress Salmonella of writing from memory: acinetobacter lwoffii: Pseudomonas fluorescens: Wickerhamomyces anomalus is 1-2:1-2:1-2:2-3; The density of the bacterium liquid that forms is 6 * 10
7Individual/more than the ml bacterium, for subsequent use.
Embodiment 2 prepares fiber from woody raw material
Take caragana microphylla as raw material, specify the Mierocrystalline cellulose preparation method when adopting woody raw material.All the other woody raw materials, for example the Mierocrystalline cellulose preparation method of willow, willow can carry out with reference to this technique.
When wherein adopting caragana microphylla, composite flora is configured according to following mass ratio:
The Tangshan Lay mattress Salmonella of writing from memory: acinetobacter lwoffii: Pseudomonas fluorescens: Wickerhamomyces anomalus is 1:2:2:2;
When adopting willow, composite flora is configured according to following mass ratio:
The Tangshan Lay mattress Salmonella of writing from memory: acinetobacter lwoffii: Pseudomonas fluorescens: Wickerhamomyces anomalus is 1:2:2:3;
When adopting willow, composite flora is configured according to following mass ratio:
The Tangshan Lay mattress Salmonella of writing from memory: acinetobacter lwoffii: Pseudomonas fluorescens: Wickerhamomyces anomalus is 2:1:2:2.
Specifically please refer to Fig. 1, Mierocrystalline cellulose preparation method's flow process is divided into three phases: preparatory stage, Mierocrystalline cellulose workshop section and byproduct workshop section.
(1) preparatory stage: 1-4
1)-2): the caragana microphylla that harvesting is returned carries out the separation of skin bar.Adopt winnowing machine or other mechanical separation all can.After the skin bar separates, skin is inputted organic feed workshop processing feed, the bar after the decortication is inputted next program.
3) with bar segment on cutting machine, length is 3-4 ㎝, and angle is for well, to increase infiltrating area.
4) the branch section input after will peeling soak in storehouse or the fermentation vat wash, cold soaking, at first with foreign material flush awaies such as raw material appearance earth, soak simultaneously, water temperature is natural temperature, the time with soak into, swollen is as the criterion 10-12h.After the liquid muddiness after repeatedly soaking, supernatant liquor can also reuse after flocculating, precipitating.Biogas is produced in the fermentation of throw out input methane-generating pit.
More than four the step can be that intermittent type also can be linkage type.
(2) Mierocrystalline cellulose workshop section: 5-12
5) discongest: with the branch section input thread-rolling machine of swollen or squeeze grind, kneading machine comes the innovation wood segment structure, makes it loose one-tenth wood wool shape, is conducive to the biological bacteria infiltration, brings into play its Degradation.
6) biological degradation: in the raw material input biological bacteria degraded storehouse or tank after will discongesting, be immersed in the bacterium liquid of embodiment 1 preparation, the mass ratio of the raw material after discongesting and bacterium liquid is 1:6, and temperature remains on 35-40 ℃, time 30-34 hour.The xylogen degradation reaction occurs under the condition of biological bacteria, brings into play its single-minded effect.
7) wet sterilization: after raw materials pretreatment is finished, with raw material from bacterium liquid, pull out, draining, be input in the steam storehouse, pass into water at atmospheric pressure steam 10-30 minute sterilization.Paste roller mill is inputted with raw material in the lower end.
8) obtaining of fiber: the raw material after will sterilizing carries out one section corase grind, becomes fibrous bundle; Above-mentioned one section corase grind is carried out two sections fine grindings, make fibrous bundle disperse into single fiber; Screening, filter through the fibrous bundle in the slurries after one section corase grind and the two sections fine grindings, defibrination makes it make single fiber again.
9) sterilization: the fiber after corase grind and fine grinding, be subjected to mechanical friction, major part is bended, torsional deformation, soaks in warm water and eliminates the fiber deflection that defibrination causes, and makes it to unfold, and then dries, sterilizes.
10) grind: the fiber after will sterilize, is again sterilized and is ground to form Mierocrystalline cellulose 11 except behind the delignification through the known routine techniques means of those skilled in the art (for example diluted alkaline method)), as food with or medical or daily-use chemical industry usefulness additive 12).
(3) byproduct stage: A-C
Isolated caragana microphylla skin contains abundant nutritive ingredient among the A, 3, and fermentation changes into ox, sheep feed.
After the liquid muddiness through repeatedly soaking, washing among the B, 4, through flocculation, precipitation, the supernatant liquor recycling, the fermentation of throw out input methane-generating pit produces biogas, passes into the coal gas boiler for dual purpose and does fuel, can play effect of energy.
C, biological organic fertilizer: natural pond slag, natural pond liquid after the methane-generating pit fermentation are the biological organic fertilizers that enriches, and liquid topdresses as farm crop and flower nutrient solution uses, and the solid granulation is made base manure and used, and all is green fertilizers.
The cellulosic physical performance index measurement result that obtains please see following table 1 for details:
Table 1
Embodiment 3 prepares Mierocrystalline cellulose from the draft raw material
Take wheat straw as raw material, specify the Mierocrystalline cellulose preparation method when adopting the draft raw material.All the other draft raw materials, for example the Mierocrystalline cellulose preparation method of straw, reed can carry out with reference to this technique.
When wherein adopting wheat straw, composite flora is configured according to following mass ratio:
The Tangshan Lay mattress Salmonella of writing from memory: acinetobacter lwoffii: Pseudomonas fluorescens: Wickerhamomyces anomalus is 2:1:2:2;
When adopting straw, composite flora is configured according to following mass ratio:
The Tangshan Lay mattress Salmonella of writing from memory: acinetobacter lwoffii: Pseudomonas fluorescens: Wickerhamomyces anomalus is 2:2:2:2;
When adopting reed, composite flora is configured according to following mass ratio:
The Tangshan Lay mattress Salmonella of writing from memory: acinetobacter lwoffii: Pseudomonas fluorescens: Wickerhamomyces anomalus is 1:1:3:2.
Specifically please refer to Fig. 2, Mierocrystalline cellulose preparation method's flow process is divided into three phases: preparatory stage, Mierocrystalline cellulose workshop section and byproduct workshop section.
(1) preparatory stage: 1,4
The segment that wheat straw is cut into 4-5cm, input soak in storehouse or the fermentation vat wash, cold soaking, at first with foreign material flush awaies such as raw material appearance earth, soak simultaneously, water temperature is natural temperature, the time with soak into, swollen is as the criterion 10-12h.After the liquid muddiness after repeatedly soaking, supernatant liquor can also reuse after flocculating, precipitating.Biogas is produced in the fermentation of throw out input methane-generating pit.
(2) Mierocrystalline cellulose workshop section: 5-12 and (three) byproduct stage: A-C are with embodiment 2, and wherein employed biological bacteria such as embodiment 1 do not repeat them here, and wherein during biological degradation, the mass ratio of the raw material after discongesting and bacterium liquid is 1:8.
The cellulosic physical performance index measurement result that obtains please see following table 2 for details:
Table 2
Although the present invention discloses as above with preferred embodiment; so it is not to limit the present invention; any person of ordinary skill in the field; without departing from the spirit and scope of the present invention; when can doing a little change and improvement, so protection scope of the present invention is as the criterion when looking the claim person of defining.
Claims (10)
1. composite flora, it comprises preserving number is that the silent Salmonella (Rheinheimera tangshanensis) of Tangshan Lay mattress, the preserving number of CGMCC No.5972 is that the acinetobacter lwoffii (Acinetobacter lwoffii) of CGMCC No.5973, Pseudomonas fluorescens (Pseudomonas fluorescens), the preserving number that preserving number is CGMCC No.5974 are the Wickerhamomyces anomalus of CGMCC No.5975.
2. the application of composite flora claimed in claim 1 in preparation additive usefulness Mierocrystalline cellulose.
3. one kind prepares the cellulosic method of additive, it is characterized in that, comprises step:
1) configuration of bacterium liquid: composite flora claimed in claim 1 is configured to the composite bacteria aqueous solution according to following mass ratio, is bacterium liquid:
The Tangshan Lay mattress Salmonella of writing from memory: acinetobacter lwoffii: Pseudomonas fluorescens: Wickerhamomyces anomalus is 1-2:1-2:1-2:2-3;
2) raw material is processed: will cut into slices after the woody raw material peeling, or the draft raw material is cut into chunks, the raw material that cuts is put into the fermentation vat swollen;
3) preparation of fiber, it comprises step:
Discongest: with raw material thread rolling and/or crowded stone roller of swollen;
Biological degradation: the raw material soaking after will discongesting is in the bacterium liquid that configures;
Wet sterilization: with the raw material after the above-mentioned biological degradation from bacterium liquid, pull out, draining, pass into water vapor sterilization;
Obtaining of fiber: the raw material after will sterilizing carries out one section corase grind, becomes fibrous bundle; Above-mentioned one section corase grind is carried out two sections fine grindings, make fibrous bundle disperse into single fiber; Screening, filter through the fibrous bundle in the slurries after one section corase grind and the two sections fine grindings, defibrination makes it make single fiber again;
Sterilization: the above-mentioned fiber that makes is soaked in warm water, then dry, sterilize;
Grind: the fiber after will sterilizing grinds to form Mierocrystalline cellulose, as additive.
4. method according to claim 3 is characterized in that, the density of the bacterium liquid that forms in the step 1) is 6 * 10
7Individual/more than the ml bacterium.
5. method according to claim 3 is characterized in that step 2) in, the woody raw material length that cuts is 3-4cm, the draft raw material length that cuts is 4-5cm.
6. according to claim 3 or 5 described methods, it is characterized in that step 2) the described swollen time is 10-12h.
7. method according to claim 3 is characterized in that, the described biological degradation temperature of step 3) remains on 35-40 ℃, time 30-34 hour.
8. method according to claim 3 is characterized in that, in the described biological degradation of step 3), the mass ratio of the raw material after discongesting and bacterium liquid is 1:6-8.
9. method according to claim 3 is characterized in that, the described steam sterilizing of step 3) is water at atmospheric pressure steam sterilizing 10-30 minute.
10. method according to claim 3 is characterized in that step 2) in soak soak solution behind the raw material through flocculation, precipitation, the supernatant liquor recycling, the fermentation of throw out input methane-generating pit produces biogas.
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| CN201210342555.5A CN103074253B (en) | 2012-09-17 | 2012-09-17 | Composite flora and additive cellulose preparation method by using same |
| PCT/CN2013/001088 WO2014040387A1 (en) | 2012-09-17 | 2013-09-16 | Biological bacterial solution and application thereof |
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| WO2014040387A1 (en) * | 2012-09-17 | 2014-03-20 | 北京天安生物科技有限公司 | Biological bacterial solution and application thereof |
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| CN101838613A (en) * | 2009-09-30 | 2010-09-22 | 河南省恒隆态生物工程股份有限公司 | Agricultural straw fast-degradation decomposition agent strain composition |
| CN102002468A (en) * | 2010-08-13 | 2011-04-06 | 东华大学 | Fluorescent pseudomonad DA4 strain as well as acquisition method and application thereof |
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| CN101838613A (en) * | 2009-09-30 | 2010-09-22 | 河南省恒隆态生物工程股份有限公司 | Agricultural straw fast-degradation decomposition agent strain composition |
| CN102002468A (en) * | 2010-08-13 | 2011-04-06 | 东华大学 | Fluorescent pseudomonad DA4 strain as well as acquisition method and application thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| WO2014040387A1 (en) * | 2012-09-17 | 2014-03-20 | 北京天安生物科技有限公司 | Biological bacterial solution and application thereof |
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