CN103074260B - Composite flora and application of the same in additive cellulose preparation - Google Patents
Composite flora and application of the same in additive cellulose preparation Download PDFInfo
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- CN103074260B CN103074260B CN201210343134.4A CN201210343134A CN103074260B CN 103074260 B CN103074260 B CN 103074260B CN 201210343134 A CN201210343134 A CN 201210343134A CN 103074260 B CN103074260 B CN 103074260B
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
- Y02A40/20—Fertilizers of biological origin, e.g. guano or fertilizers made from animal corpses
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/30—Fuel from waste, e.g. synthetic alcohol or diesel
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
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- Micro-Organisms Or Cultivation Processes Thereof (AREA)
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Abstract
The present invention relates to a composite flora and an application of the composite flora in additive cellulose preparation. The composite flora comprises rheinheimera tangshanensis with a preservation number of CGMCC No.5972, acinetobacter lwoffii with a preservation number of CGMCC No.5973, and pseudomonas fluorescens with a preservation number of CGMCC No.5974. The process for preparing the additive cellulose comprises the following steps: preparing a bacterial liquid; treating raw materials; and preparing fibers, wherein fiber preparation comprises fiber kneading or squeezing rolling, biodegradation, steam sterilization, fiber obtaining, sterilization and grinding. According to the present invention, the method does not pollute the environment, a waste liquid is directly converted into an organic fertilizer so as to achieve zero emission and zero pollution, the biological treatment process provides a protection effect for fibers, advantages of low production cost and high economic benefits are provided compared with the traditional chemical method, and characteristics of energy source saving and environmental protection are provided.
Description
Technical field
The present invention relates to a kind of additive Mierocrystalline cellulose, relate in particular to a kind of composite flora and preparing the application in Mierocrystalline cellulose for additive.
Background technology
Mierocrystalline cellulose is compound of polysaccharide, is the most valuable natural reproducible resources of the mankind.Cellulose chemistry and industry started from before more than 160 year, polymer chemistry birth and the main study subject of developing period, cellulosic major physiological effect is absorption large quantity of moisture, increase ight soil amount, promote intestinal peristalsis, accelerate the excretion of ight soil, the residence time of carcinogenic substance in enteron aisle shortened, the pessimal stimulation of enteron aisle is reduced, thus can prevention of intestinal carcinogenesis.In prior art, prepare Mierocrystalline cellulose, how by chemical process, the waste liquor contamination sublimity that chemical process produces in producing, damage to land, polluted air, and also it is high to consume energy, and power consumption and water consumption are large.Do not meet national energy-saving and reduce discharging policy.Material can not reach Efficient Cycle recycling.Chemicals cannot be separated from waste liquid, and organism and chemicals mix, and organism also cannot be reused, and causes a large amount of losses.
Therefore, be necessary to develop biological cellulose technology processed, fundamentally solve an above-mentioned pollution difficult problem, energy-saving and emission-reduction, water-saving, reduces production costs and improves the rate of utilization of material.
Summary of the invention
The object of the invention is to overcome the cellulosic above-mentioned defect of chemical legal system of prior art, a kind of new Mierocrystalline cellulose biological bacteria of preparing is provided.Contriver, by long screening operation, has obtained and has been suitable for preparing the cellulosic biological bacteria that can realize foregoing invention object.
Particularly, the invention provides a kind of composite flora, it comprises preserving number is that the silent Salmonella (Rheinheimera tangshanensis) of Tangshan Lay mattress, the preserving number of CGMCC No.5972 is the acinetobacter lwoffii (Acinetobacter lwoffii) of CGMCC No.5973, the Pseudomonas fluorescens (Pseudomonas fluorescens) that preserving number is CGMCC No.5974.
Described composite flora can be used in prepares additive Mierocrystalline cellulose.
The present invention also provides a kind of cellulosic method of additive of preparing, and it mainly comprises step:
1) configuration of bacterium liquid: above-mentioned composite flora is configured to the composite bacteria aqueous solution according to following mass ratio, is bacterium liquid:
The Tangshan Lay mattress Salmonella of writing from memory: acinetobacter lwoffii: Pseudomonas fluorescens is 1-2:1-2:1-2;
2) raw material is processed: by section after woody raw material peeling, or draft raw material is cut into chunks, the raw material cutting is put into fermentation vat swollen;
3) preparation of fiber, it comprises step:
Discongest: by the raw material thread rolling of swollen and/or crowded stone roller;
Biological degradation: by the raw material soaking after discongesting in the bacterium liquid configuring;
Wet sterilization: by the raw material after above-mentioned biological degradation from bacterium liquid, pull out, draining, pass into water vapor sterilizing;
Obtaining of fiber: the raw material after sterilizing is carried out to one section of corase grind, become fibrous bundle; Above-mentioned one section of corase grind is carried out to two sections of fine grindings, make fibrous bundle disperse into single fiber; Fibrous bundle in screening, the slurries of filtration after one section of corase grind and two sections of fine grindings, defibrination makes it make single fiber again;
Sterilizing: the above-mentioned fiber making is soaked in warm water, then oven dry, sterilizing;
Grind: the fiber after sterilizing is ground to form to Mierocrystalline cellulose, as additive.
The density of the bacterium liquid wherein, forming in step 1) be 6,000 ten thousand/more than ml bacterium.
Wherein, step 2) in, the woody raw material length cutting is 3-4cm, the draft raw material length cutting is 4-5cm.The described swollen time is 10-12h.
Wherein, the biological degradation temperature described in step 3) remains on 35-40 ℃, time 32-36 hour.The mass ratio of the raw material after discongesting and bacterium liquid is 1:6-8.Described steam sterilizing is water at atmospheric pressure steam sterilizing 10-30 minute.
Further, step 2) in, soak soak solution after raw material through flocculation, precipitation, supernatant liquor recycling, the fermentation of throw out input methane-generating pit produces biogas.
Biological method provided by the invention is prepared cellulosic advantage: 1) free from environmental pollution: waste liquid directly changes into organic fertilizer, reaches zero release, and zero pollutes.2) biological method can play a protective role to fiber, compares with traditional chemical process, and present method can all reclaim full fiber and half fiber, has therefore improved yield.3) biological method carries out lignin degradation under normal pressure, energy-conservation, reduction of discharging, low-carbon (LC).4) production cost is low, and economic benefit is high.
Byproduct of the present invention is transported to settling tank flocculation, precipitation, and supernatant liquor returns to second stage employ, remakes presoak and uses.In throw out, contain the nutrient for plants such as the gas chromatographies such as abundant xylogen and N, P, K, throw out mixes with old bacterium liquid (the raw material thickness bacterium liquid of repeatedly degrading, also contains N, P, K, Fe and trace element) again, acidifying, then together enter in marsh gas fermentation pool, produce biogas.By the Boiler Ash mixing granulation of natural pond slag, natural pond liquid and pulverizing, granulation fertilizer, finally dispatches from the factory, and realizes zero release.
The present invention is by the biological bacteria that provides above-mentioned contriver to obtain through long creation work, the further perfect cellulosic technology of existing preparation, reduced the reaction times, improved the purity and the yield that make fiber, this technology can be applied on a large scale in actual production.Applying biological bacterium of the present invention at short notice in degrading plant body xylogen obtain fiber, byproduct twice transformation becomes biogas, biogas coal supply, gas boiler for dual purpose combustion heating are saved coal consumption.Finally, natural pond slag is made organic fertilizer, has formed the economic circulation new model of " material organic transformation ", reaches no waste discharge, namely zero release.Fundamentally solved a pollution difficult problem for chemical preparation fiber of the prior art.Energy-saving and emission-reduction, water-saving, has reduced production cost, has improved the rate of utilization of material.
For above and other objects of the present invention, feature and advantage can be become apparent, preferred embodiment cited below particularly, and coordinate accompanying drawing, be described in detail below.
Accompanying drawing explanation
Fig. 1 prepares the cellulosic schema of additive according to one embodiment of the invention;
Fig. 2 be according to another embodiment of the present invention prepare the cellulosic schema of additive.
Embodiment
The configuration of embodiment 1 bacterium liquid
The biological bacteria adopting in the present invention is in China Committee for Culture Collection of Microorganisms common micro-organisms center (CGMCC April 6 in 2012, No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City) preservation, it comprises preserving number is that the silent Salmonella (Rheinheimera tangshanensis) of Tangshan Lay mattress, the preserving number of CGMCC No.5972 is the acinetobacter lwoffii (Acinetobacter lwoffii) of CGMCC No.5973, the Pseudomonas fluorescens (Pseudomonas fluorescens) that preserving number is CGMCC No.5974.
Above-mentioned composite flora is configured to the composite bacteria aqueous solution according to following mass ratio, is bacterium liquid:
The Tangshan Lay mattress Salmonella of writing from memory: acinetobacter lwoffii: Pseudomonas fluorescens: be 1-2:1-2:1-2; The density of the bacterium liquid forming be 6,000 ten thousand/more than ml bacterium, standby.
Embodiment 2 prepares fiber from woody raw material
Take caragana microphylla as raw material, illustrate the Mierocrystalline cellulose preparation method while adopting woody raw material.All the other woody raw materials, for example the Mierocrystalline cellulose preparation method of willow, willow can carry out with reference to this technique.While wherein adopting caragana microphylla, composite flora is configured according to following mass ratio: the Tangshan Lay mattress Salmonella of writing from memory: acinetobacter lwoffii: Pseudomonas fluorescens is 1:2:2; While adopting willow, composite flora is configured according to following mass ratio: the Tangshan Lay mattress Salmonella of writing from memory: acinetobacter lwoffii: Pseudomonas fluorescens is 1:2:2; While adopting willow, composite flora is configured according to following mass ratio: the Tangshan Lay mattress Salmonella of writing from memory: acinetobacter lwoffii: Pseudomonas fluorescens is 2:1:2.
Specifically please refer to Fig. 1, Mierocrystalline cellulose preparation method's flow process is divided into three phases: preparatory stage, Mierocrystalline cellulose workshop section and byproduct workshop section.
(1) preparatory stage: 1-4
1)-2): it is separated that the caragana microphylla that harvesting is returned carries out skin bar.Adopt winnowing machine or other mechanical separation all can.After the separation of skin bar, skin is inputted to organic feed workshop processing feed, the bar after decortication is inputted next program.
3) by bar segment on cutting machine, length is 3-4 ㎝, and angle is for well, to increase infiltrating area.
4) the branch section input after decortication is soaked in storehouse or fermentation vat wash, cold soaking, first the foreign material such as raw material appearance earth are washed away, soak simultaneously, water temperature is natural temperature, the time soaking into, swollen is as the criterion, 10-12h.After liquid muddiness after repeatedly soaking, after flocculating, precipitating, supernatant liquor can also reuse.The fermentation of throw out input methane-generating pit, produces biogas.
Above four steps can be that intermittent type can be also linkage type.
(2) Mierocrystalline cellulose workshop section: 5-12
5) discongest: by the branch section input thread-rolling machine of swollen or squeeze grind, kneading machine carrys out innovation wood segment structure, makes it loose one-tenth wood wool shape, is conducive to biological bacteria infiltration, brings into play its Degradation.
6) biological degradation: by the raw material input biological bacteria degraded storehouse after discongesting or tank, be immersed in the bacterium liquid of embodiment 1 preparation, the mass ratio of the raw material after discongesting and bacterium liquid is 1:6, and temperature remains on 35-40 ℃, time 32-36 hour.Under the condition of biological bacteria, there is xylogen degradation reaction, bring into play its single-minded effect.
7) wet sterilization: after raw materials pretreatment completes, by raw material from bacterium liquid, pull out, draining, be input in steam storehouse, pass into the sterilizing in 10-30 minute of water at atmospheric pressure steam.Paste roller mill is inputted by raw material in lower end.
8) obtaining of fiber: the raw material after sterilizing is carried out to one section of corase grind, become fibrous bundle; Above-mentioned one section of corase grind is carried out to two sections of fine grindings, make fibrous bundle disperse into single fiber; Fibrous bundle in screening, the slurries of filtration after one section of corase grind and two sections of fine grindings, defibrination makes it make single fiber again.
9) sterilizing: the fiber after corase grind and fine grinding, be subject to mechanical friction, major part is bended, torsional deformation, in warm water, soaks and eliminates the fiber deflection that defibrination causes, and makes it to unfold, then oven dry, sterilizing.
10) grind: by the fiber after sterilizing for example, through the known routine techniques means of those skilled in the art (diluted alkaline method) except after delignification, again sterilizing and grind to form Mierocrystalline cellulose 11), as food with or medical or additive 12 for daily-use chemical industry).
(3) byproduct stage: A-C
In A, 3, isolated caragana microphylla skin contains abundant nutritive ingredient, and fermentation changes into ox, sheep feed.
After liquid muddiness through repeatedly soaking, washing in B, 4, through flocculation, precipitation, supernatant liquor recycling, the fermentation of throw out input methane-generating pit produces biogas, passes into coal gas boiler for dual purpose and does fuel, can play effect of energy.
C, biological organic fertilizer: natural pond slag, natural pond liquid after methane-generating pit fermentation are abundant biological organic fertilizers, liquid topdresses as farm crop and flower nutrient solution is used, and solid granulation is made base manure and is used, and is all green fertilizers.
The cellulosic physical performance index measurement result obtaining please refer to following table 1:
Table 1
Embodiment 3 prepares Mierocrystalline cellulose from draft raw material
Take wheat straw as raw material, illustrate the Mierocrystalline cellulose preparation method while adopting draft raw material.All the other draft raw materials, for example the Mierocrystalline cellulose preparation method of straw, reed can carry out with reference to this technique.
While wherein adopting wheat straw, composite flora is configured according to following mass ratio: the Tangshan Lay mattress Salmonella of writing from memory: acinetobacter lwoffii: Pseudomonas fluorescens is 2:1:2; While adopting straw, composite flora is configured according to following mass ratio: the Tangshan Lay mattress Salmonella of writing from memory: acinetobacter lwoffii: Pseudomonas fluorescens is 2:2:2; While adopting reed, composite flora is configured according to following mass ratio: the Tangshan Lay mattress Salmonella of writing from memory: acinetobacter lwoffii: Pseudomonas fluorescens is 1:1:3.
Specifically please refer to Fig. 2, Mierocrystalline cellulose preparation method's flow process is divided into three phases: preparatory stage, Mierocrystalline cellulose workshop section and byproduct workshop section.
(1) preparatory stage: 1,4
Wheat straw is cut into the segment of 4-5cm, input soak in storehouse or fermentation vat wash, cold soaking, first the foreign material such as raw material appearance earth are washed away, soak simultaneously, water temperature is natural temperature, the time soaking into, swollen is as the criterion, 10-12h.After liquid muddiness after repeatedly soaking, after flocculating, precipitating, supernatant liquor can also reuse.The fermentation of throw out input methane-generating pit, produces biogas.
(2) Mierocrystalline cellulose workshop section: 5-12 and (three) byproduct stage: A-C be with embodiment 2, and the biological bacteria wherein used does not repeat them here as embodiment 1, and wherein during biological degradation, the mass ratio of the raw material after discongesting and bacterium liquid is 1:8.
The cellulosic physical performance index measurement result obtaining please refer to following table 2:
Table 2
Although the present invention discloses as above with preferred embodiment; so it is not in order to limit the present invention; any person of ordinary skill in the field; without departing from the spirit and scope of the present invention; when doing a little change and improvement, so protection scope of the present invention is when being as the criterion depending on the claim person of defining.
Claims (5)
1. prepare the cellulosic method of additive, it is characterized in that, comprise step:
1) configuration of bacterium liquid: by preserving number be CGMCC No.5972 the silent Salmonella of Tangshan Lay mattress (
rheinheimera tangshanensis), preserving number be CGMCC No.5973 acinetobacter lwoffii (
acinetobacter lwoffii), preserving number be CGMCC No.5974 Pseudomonas fluorescens (
pseudomonas fluorescens) according to following mass ratio, be configured to the composite bacteria aqueous solution, be bacterium liquid:
The Tangshan Lay mattress Salmonella of writing from memory: acinetobacter lwoffii: Pseudomonas fluorescens is 1-2:1-2:1-2, the density of bacterium liquid be 6,000 ten thousand/more than ml bacterium;
2) raw material is processed: by section after woody raw material peeling, or draft raw material is cut into chunks, the raw material cutting is put into fermentation vat swollen, wherein, described woody raw material is caragana microphylla, willow or willow, and draft raw material is wheat straw, straw or reed;
3) preparation of fiber, it comprises step:
Discongest: by the raw material thread rolling of swollen and/or crowded stone roller;
Biological degradation: in the bacterium liquid configuring, the mass ratio of the raw material after discongesting and bacterium liquid is 1:6-8 by the raw material soaking after discongesting, and degradation temperature is 35-40 ℃, and degradation time is 32-36 hour;
Wet sterilization: by the raw material after above-mentioned biological degradation from bacterium liquid, pull out, draining, pass into water vapor sterilizing;
Obtaining of fiber: the raw material after sterilizing is carried out to one section of corase grind, become fibrous bundle; Above-mentioned one section of corase grind is carried out to two sections of fine grindings, make fibrous bundle disperse into single fiber; Fibrous bundle in screening, the slurries of filtration after one section of corase grind and two sections of fine grindings, defibrination makes it make single fiber again;
Sterilizing: the above-mentioned fiber making is soaked in warm water, then oven dry, sterilizing;
Grind: the fiber after sterilizing is ground to form to Mierocrystalline cellulose, as additive.
2. method according to claim 1, is characterized in that step 2) in, the woody raw material length cutting is 3-4cm, the draft raw material length cutting is 4-5cm.
3. method according to claim 1 and 2, is characterized in that step 2) the described swollen time is 10-12h.
4. method according to claim 1, is characterized in that, the steam sterilizing described in step 3) is water at atmospheric pressure steam sterilizing 10-30 minute.
5. method according to claim 1, is characterized in that step 2) in soak soak solution after raw material through flocculation, precipitation, supernatant liquor recycling, the fermentation of throw out input methane-generating pit produces biogas.
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| CN201210343134.4A CN103074260B (en) | 2012-09-17 | 2012-09-17 | Composite flora and application of the same in additive cellulose preparation |
| PCT/CN2013/001095 WO2014040394A1 (en) | 2012-09-17 | 2013-09-16 | Complex microbial flora and application thereof |
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| CN201210343134.4A CN103074260B (en) | 2012-09-17 | 2012-09-17 | Composite flora and application of the same in additive cellulose preparation |
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Citations (2)
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|---|---|---|---|---|
| CN102002468A (en) * | 2010-08-13 | 2011-04-06 | 东华大学 | Fluorescent pseudomonad DA4 strain as well as acquisition method and application thereof |
| CN102113622A (en) * | 2011-03-17 | 2011-07-06 | 南开大学 | Straw and pot ale mixed fermented feed and production method thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102002468A (en) * | 2010-08-13 | 2011-04-06 | 东华大学 | Fluorescent pseudomonad DA4 strain as well as acquisition method and application thereof |
| CN102113622A (en) * | 2011-03-17 | 2011-07-06 | 南开大学 | Straw and pot ale mixed fermented feed and production method thereof |
Non-Patent Citations (4)
| Title |
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| 张晓霞.水稻根相关细菌的多相分类及Rheinheimera tangshanensis sp.nov.的描述.《中国博士学位论文全文数据库 农业科技辑》》.2009,(第10期),摘要，引言第5页. |
| 水稻根相关细菌的多相分类及Rheinheimera tangshanensis sp.nov.的描述;张晓霞;《中国博士学位论文全文数据库 农业科技辑》》;20091015(第10期);摘要，引言第5页 * |
| 苎麻微生物脱胶菌株筛选及脱胶效果评价;陈杨栋 等;《纺织学报》;20100531;第31卷(第5期);69页摘要，72页右栏 * |
| 陈杨栋 等.苎麻微生物脱胶菌株筛选及脱胶效果评价.《纺织学报》.2010,第31卷(第5期),69页摘要，72页右栏. |
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