CN103074269B - A kind of composite flora and application thereof preparing cellulosic additive for biology - Google Patents
A kind of composite flora and application thereof preparing cellulosic additive for biology Download PDFInfo
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- CN103074269B CN103074269B CN201210344067.8A CN201210344067A CN103074269B CN 103074269 B CN103074269 B CN 103074269B CN 201210344067 A CN201210344067 A CN 201210344067A CN 103074269 B CN103074269 B CN 103074269B
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
- Y02A40/20—Fertilizers of biological origin, e.g. guano or fertilizers made from animal corpses
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/30—Fuel from waste, e.g. synthetic alcohol or diesel
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
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Abstract
The present invention relates to a kind of composite flora and the application thereof of preparing cellulosic additive for biology.Does described composite flora comprise preserving number CGMCC? the genus bacillus of No.5971, CGMCC? is the Tangshan Lay mattress of No.5972 write from memory Salmonella, CGMCC? the Wickerhamomyces of No.5975? anomalus.The cellulosic technological process of additive of preparing provided by the invention comprises step: the configuration of bacterium liquid; Feedstock treating; The preparation of fiber, it comprises discongests, biological degradation, wet sterilization, the acquisition of fiber, sterilizing, grinding.Aforesaid method is free from environmental pollution, and waste liquid directly changes into organic fertilizer, reaches zero release, no pollution.Biological treatment process can play a protective role to fiber, and compared with traditional chemical process, production cost is low, high financial profit.Not only saved the energy but also environmental protection.
Description
Technical field
The present invention relates to a kind of additive Mierocrystalline cellulose, particularly relate to a kind of composite flora and the application thereof of preparing cellulosic additive for biology.
Background technology
Mierocrystalline cellulose is compound of polysaccharide, is the most valuable natural reproducible resources of the mankind.Before cellulose chemistry and industry start from more than 160 year, polymer chemistry birth and the main study subject of developing period, cellulosic major physiological effect is absorption large quantity of moisture, increase ight soil amount, promote intestinal peristalsis, accelerate the excretion of ight soil, make the shorter residence time of carcinogenic substance in enteron aisle, the pessimal stimulation of enteron aisle is reduced, thus can prevention of intestinal carcinogenesis.Prepare Mierocrystalline cellulose in prior art, how by chemical process, the waste liquor contamination sublimity that chemical process produces in producing, damage to land, polluted air, and it is high to consume energy, power consumption and water consumption large.Do not meet national energy-saving and reduce discharging policy.Material can not reach Efficient Cycle recycling.Chemicals cannot be separated from waste liquid, and organism and chemicals mix, and organism also cannot be reused, and causes a large amount of loss.
Therefore, be necessary to develop biological cellulose technology processed, fundamentally solve an above-mentioned pollution difficult problem, energy-saving and emission-reduction, water-saving, reduce production cost and the rate of utilization of raising material.
Summary of the invention
The object of the invention is to overcome the cellulosic above-mentioned defect of chemical legal system of prior art, provide a kind of newly prepare Mierocrystalline cellulose biological bacteria.Contriver, by long screening operation, obtains and is suitable for preparing the cellulosic biological bacteria that can realize foregoing invention object.
Particularly, the invention provides a kind of composite flora, the Tangshan Lay mattress that it comprises genus bacillus (Bacillussp.) that preserving number is CGMCCNo.5971, preserving number is CGMCCNo.5972 write from memory Salmonella (Rheinheimeratangshanensis), preserving number be this Wickerhamomycesanomalus bacterium of Wickerhamomycesanomalus(of CGMCCNo.5975 in malt juice liquid medium 25 DEG C cultivate three days, cell is spherical, avette, sausage shape, size is (4.8-14.4) × (3.6-7.2) μm, has precipitation to be formed.25 DEG C, wort agar inclined-plane is cultivated one month, bacterium colony cheese shape, oyster white, and surface smoothing is not reflective, edge root shape.Corn Meal Agar Dalmau slat chain conveyor, produces pseudohypha).
Described composite flora can be used in preparing additive Mierocrystalline cellulose.
The present invention also provides one to prepare the cellulosic method of additive, and it mainly comprises step:
1) configuration of bacterium liquid: above-mentioned composite flora is configured to the composite bacteria aqueous solution according to following mass ratio, is bacterium liquid:
Genus bacillus: Lay mattress Salmonella: the Wickerhamomycesanomalus that writes from memory in Tangshan is 2-3:1-2:2-3;
2) Feedstock treating: by section after woody raw material peeling, or herbaceous material is cut into chunks, the raw material cut is put into fermentation vat swollen;
3) preparation of fiber, it comprises step:
Discongest: by the raw material thread rolling of swollen and/or crowded stone roller;
Biological degradation: by the raw material soaking after discongesting in the bacterium liquid configured;
Wet sterilization: to be pulled out from bacterium liquid by the raw material after above-mentioned biological degradation, draining, passes into water vapor sterilizing;
The acquisition of fiber: the raw material after sterilizing is carried out one section of corase grind, become fibrous bundle; Above-mentioned one section of corase grind is carried out two sections of fine grindings, makes fibrous bundle be dispersed into single fiber; Screening, be filtered through one section corase grind and two sections of fine grindings after slurries in fibrous bundle, defibrination makes it make single fiber again;
Sterilizing: above-mentioned obtained fiber is soaked in warm water, then oven dry, sterilizing;
Grinding: the fiber after sterilizing is ground to form Mierocrystalline cellulose, as additive.
Wherein, the density of the bacterium liquid formed in step 1) is more than 6,000 ten thousand/ml bacterium.
Wherein, step 2) in, the woody raw material length cut is 3-4cm, and the herbaceous material length cut is 4-5cm.The described swollen time is 10-12h.
Wherein, the biological degradation temperature described in step 3) remains on 35-40 DEG C, time 32-36 hour.The mass ratio of the raw material after discongesting and bacterium liquid is 1:6-8.Described steam sterilizing is water at atmospheric pressure steam sterilizing 10-30 minute.
Further, step 2) in soak the soak solution after raw material through flocculation, precipitation, supernatant liquor recycling, the fermentation of throw out input methane-generating pit produces biogas.
Biological method provided by the invention prepares cellulosic advantage: 1) free from environmental pollution: waste liquid directly changes into organic fertilizer, reaches zero release, no pollution.2) biological method can play a protective role to fiber, and compared with traditional chemical process, Whole fiber and half fiber can all reclaim by present method, therefore improve yield.3) biological method carries out lignin degradation at ambient pressure, energy-conservation, reduce discharging, low-carbon (LC).4) production cost is low, high financial profit.
Byproduct of the present invention is transported to settling tank flocculation, precipitation, and supernatant liquor returns second stage employ, remakes presoak and uses.Containing nutrient for plants such as gas chromatography and N, P, K such as abundant xylogen in throw out, throw out mixes, acidifying with old bacterium liquid (raw material thickness bacterium liquid of repeatedly degrading, also containing N, P, K, Fe and trace element) again, then together enter in marsh gas fermentation pool, produce biogas.By the Boiler Ash mixing granulation of natural pond slag, natural pond liquid and pulverizing, make granular organic fertilizer, finally dispatch from the factory, realize zero release.
The biological bacteria that the present invention obtains through creating work for a long time by providing above-mentioned contriver, the perfect further cellulosic technology of existing preparation, reduce the reaction times, improve purity and the yield of obtained fiber, this technology can be applied on a large scale in actual production.Applying biological bacterium of the present invention at short notice in degrading plant body xylogen obtain fiber, byproduct twice transformation becomes biogas, biogas coal supply, gas boiler for dual purpose combustion heating, saves coal consumption.Finally, natural pond slag makes organic fertilizer, defines the economic circulation new model of " material organic transformation ", reaches no waste discharge, namely zero release.Fundamentally solve a pollution difficult problem for chemical preparation fiber of the prior art.Energy-saving and emission-reduction, water-saving, reduces production cost, improves the rate of utilization of material.
For above and other objects of the present invention, feature and advantage can be become apparent, preferred embodiment cited below particularly, and coordinate accompanying drawing, be described in detail below.
Accompanying drawing explanation
Fig. 1 prepares the cellulosic schema of additive according to one embodiment of the invention;
Fig. 2 be according to another embodiment of the present invention prepare the cellulosic schema of additive.
Embodiment
The configuration of embodiment 1 bacterium liquid
The biological bacteria adopted in the present invention on April 6th, 2012 at China Committee for Culture Collection of Microorganisms's common micro-organisms center (CGMCC, No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City) preservation, write from memory Salmonella (Rheinheimeratangshanensis), preserving number of the Tangshan Lay mattress that it comprises genus bacillus (Bacillussp.) that preserving number is CGMCCNo.5971, preserving number is CGMCCNo.5972 is the Wickerhamomycesanomalus of CGMCCNo.5975.
Above-mentioned composite flora is configured to the composite bacteria aqueous solution according to following mass ratio, is bacterium liquid:
Genus bacillus: Lay mattress Salmonella: the Wickerhamomycesanomalus that writes from memory in Tangshan is 2-3:1-2:2-3; The density of the bacterium liquid formed is more than 6,000 ten thousand/ml bacterium, for subsequent use.
Embodiment 2 prepares fiber from woody raw material
Be raw material with caragana microphylla, illustrate Mierocrystalline cellulose preparation method when adopting woody raw material.All the other woody raw materials, the Mierocrystalline cellulose preparation method of such as willow, willow can refer to this technique and carries out.When wherein adopting caragana microphylla, composite flora is configured according to following mass ratio: genus bacillus: Lay mattress Salmonella: the Wickerhamomycesanomalus that writes from memory in Tangshan is 3:1:2; When adopting willow, composite flora is configured according to following mass ratio: genus bacillus: Lay mattress Salmonella: the Wickerhamomycesanomalus that writes from memory in Tangshan is 2:1:3; When adopting willow, composite flora is configured according to following mass ratio: genus bacillus: Lay mattress Salmonella: the Wickerhamomycesanomalus that writes from memory in Tangshan is 3:2:2.
Specifically please refer to Fig. 1, the flow process of Mierocrystalline cellulose preparation method is divided into three phases: preparatory stage, Mierocrystalline cellulose workshop section and byproduct workshop section.
(1) preparatory stage: 1-4
1)-2): the caragana microphylla that harvesting is returned is carried out the separation of skin bar.Adopt winnowing machine or other mechanical separation.After skin bar is separated, skin is inputted organic feed workshop processing feed, the bar after decortication inputs next program.
3) by bar segment on cutting machine, length is 3-4 ㎝, angle as well, to increase infiltrating area.
4) the branch section input after decortication is soaked in storehouse or fermentation vat carry out washing, cold soaking, first washed away by the foreign material such as raw material appearance earth, soak, water temperature is natural temperature simultaneously, the time to soak into, swollen is as the criterion, 10-12h.After liquid muddiness after repeatedly soaking, carry out flocculating, supernatants after precipitation can also reuse.The fermentation of throw out input methane-generating pit, produces biogas.
Above four steps can be intermittent types also can be linkage type.
(2) Mierocrystalline cellulose workshop section: 5-12
5) discongest: the branch section of swollen input thread-rolling machine or squeeze is ground, kneading machine carrys out innovation wood segment structure, makes it loose one-tenth wood wool shape, be conducive to biological bacteria infiltration, play its Degradation.
6) biological degradation: the raw material input biological bacteria after discongesting degraded in storehouse or tank, be immersed in the bacterium liquid of embodiment 1 preparation, the mass ratio of the raw material after discongesting and bacterium liquid is 1:6, and temperature remains on 35-40 DEG C, time 32-36 hour.Under the condition of biological bacteria, there is xylogen degradation reaction, play its single-minded effect.
7) wet sterilization: after raw materials pretreatment completes, pulls out raw material from bacterium liquid, draining, is input in steam cabin, passes into the sterilizing in 10-30 minute of water at atmospheric pressure steam.Raw material is inputted paste roller mill by lower end.
8) acquisition of fiber: the raw material after sterilizing is carried out one section of corase grind, become fibrous bundle; Above-mentioned one section of corase grind is carried out two sections of fine grindings, makes fibrous bundle be dispersed into single fiber; Screening, be filtered through one section corase grind and two sections of fine grindings after slurries in fibrous bundle, defibrination makes it make single fiber again.
9) sterilizing: the fiber after corase grind and fine grinding, by mechanical friction, major part is bended, torsional deformation, soaks the fiber flexure eliminated defibrination and cause, make it to unfold, then oven dry, sterilizing in warm water.
10) grind: by the fiber after sterilizing through the known routine techniques means of those skilled in the art (such as diluted alkaline method) except after delignification, again sterilizing and grind to form Mierocrystalline cellulose 11), as food with or medical or daily-use chemical industry additive 12).
(3) byproduct stage: A-C
In A, 3, isolated caragana microphylla skin contains abundant nutritive ingredient, and microbe conversion becomes ox, sheep feed.
After liquid muddiness through repeatedly soaking, washing in B, 4, through flocculation, precipitation, supernatant liquor recycling, the fermentation of throw out input methane-generating pit produces biogas, passes into coal gas boiler for dual purpose and does fuel, can play effect of energy.
C, biological organic fertilizer: the natural pond slag after methane-generating pit fermentation, natural pond liquid are abundant biological organic fertilizers, liquid topdresses as farm crop and flower nutrient solution uses, and solid granulation is made base manure and used, and is all green fertilizers.
The cellulosic physical performance index measurement result obtained please refer to following table 1:
Table 1
Embodiment 3 prepares Mierocrystalline cellulose from herbaceous material
Be raw material with wheat straw, illustrate Mierocrystalline cellulose preparation method when adopting herbaceous material.All the other herbaceous material, the Mierocrystalline cellulose preparation method of such as straw, reed can refer to this technique and carries out.
When wherein adopting wheat straw, composite flora is configured according to following mass ratio: genus bacillus: Lay mattress Salmonella: the Wickerhamomycesanomalus that writes from memory in Tangshan is 3:2:2; When adopting straw, composite flora is configured according to following mass ratio: genus bacillus: Lay mattress Salmonella: the Wickerhamomycesanomalus that writes from memory in Tangshan is 2:2:2; When adopting reed, composite flora is configured according to following mass ratio: genus bacillus: Lay mattress Salmonella: the Wickerhamomycesanomalus that writes from memory in Tangshan is 3:1:2.
Specifically please refer to Fig. 2, the flow process of Mierocrystalline cellulose preparation method is divided into three phases: preparatory stage, Mierocrystalline cellulose workshop section and byproduct workshop section.
(1) preparatory stage: 1,4
Wheat straw is cut into the segment of 4-5cm, input soak in storehouse or fermentation vat carry out washing, cold soaking, first washed away by the foreign material such as raw material appearance earth, soak, water temperature is natural temperature simultaneously, the time to soak into, swollen is as the criterion, 10-12h.After liquid muddiness after repeatedly soaking, carry out flocculating, supernatants after precipitation can also reuse.The fermentation of throw out input methane-generating pit, produces biogas.
(2) Mierocrystalline cellulose workshop section: 5-12 and (three) byproduct stage: A-C is with embodiment 2, and wherein used biological bacteria such as embodiment 1 does not repeat them here, and wherein during biological degradation, the mass ratio of the raw material after discongesting and bacterium liquid is 1:8.
The cellulosic physical performance index measurement result obtained please refer to following table 2:
Table 2
Although the present invention discloses as above with preferred embodiment; so itself and be not used to limit the present invention; any person of ordinary skill in the field; without departing from the spirit and scope of the present invention; when doing a little change and improvement, therefore protection scope of the present invention is when being as the criterion depending on the claim person of defining.
Claims (4)
1. prepare the cellulosic method of additive, it is characterized in that, comprise step:
1) configuration of bacterium liquid: be the Bacillussp. of CGMCCNo.5971 by preserving number, preserving number is the Rheinheimeratangshanensis of CGMCCNo.5972, Wickerhamomycesanomalus that preserving number is CGMCCNo.5975 is configured to the composite bacteria aqueous solution according to following mass ratio, be bacterium liquid:
Bacillussp.:Rheinheimeratangshanensis:Wickerhamomycesano malus is 2-3: 1-2: 2-3, and the density of bacterium liquid is more than 6,000 ten thousand/ml bacterium;
2) Feedstock treating: by section after woody raw material peeling, or herbaceous material is cut into chunks, the raw material cut is put into fermentation vat swollen, the described swollen time is 10-12h, wherein, described woody raw material is caragana microphylla, willow or willow, and herbaceous material is wheat straw, straw or reed;
3) preparation of fiber, it comprises step:
Discongest: by the raw material thread rolling of swollen and/or crowded stone roller;
Biological degradation: by the raw material soaking after discongesting in the bacterium liquid configured, the mass ratio of the raw material after discongesting and bacterium liquid is 1: 6-8, and degradation temperature is 35-40 DEG C, and degradation time is 32-36 hour;
Wet sterilization: to be pulled out from bacterium liquid by the raw material after above-mentioned biological degradation, draining, passes into water vapor sterilizing;
The acquisition of fiber: the raw material after sterilizing is carried out one section of corase grind, become fibrous bundle; Above-mentioned one section of corase grind is carried out two sections of fine grindings, makes fibrous bundle be dispersed into single fiber; Screening, be filtered through one section corase grind and two sections of fine grindings after slurries in fibrous bundle, defibrination makes it make single fiber again;
Sterilizing: above-mentioned obtained fiber is soaked in warm water, then oven dry, sterilizing;
Grinding: the fiber after sterilizing is ground to form Mierocrystalline cellulose, as additive.
2. method according to claim 1, is characterized in that, step 2) in, the woody raw material length cut is 3-4cm, and the herbaceous material length cut is 4-5cm.
3. method according to claim 1, is characterized in that, step 3) described in steam sterilizing be water at atmospheric pressure steam sterilizing 10-30 minute.
4. method according to claim 1, is characterized in that, step 2) in soak the soak solution after raw material through flocculation, precipitation, supernatant liquor recycling, the fermentation of throw out input methane-generating pit produces biogas.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201210344067.8A CN103074269B (en) | 2012-09-17 | 2012-09-17 | A kind of composite flora and application thereof preparing cellulosic additive for biology |
| PCT/CN2013/001091 WO2014040390A1 (en) | 2012-09-17 | 2013-09-16 | Complex microbial flora and application thereof |
| CA2885486A CA2885486A1 (en) | 2012-09-17 | 2013-09-16 | Complex microbial flora and application thereof |
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| CN201210344067.8A CN103074269B (en) | 2012-09-17 | 2012-09-17 | A kind of composite flora and application thereof preparing cellulosic additive for biology |
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101838613A (en) * | 2009-09-30 | 2010-09-22 | 河南省恒隆态生物工程股份有限公司 | Agricultural straw fast-degradation decomposition agent strain composition |
| CN102174399A (en) * | 2010-12-31 | 2011-09-07 | 广西赛尔纸业有限公司 | Method for preparing herbal biological fiber and biological paper pulp |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101838613A (en) * | 2009-09-30 | 2010-09-22 | 河南省恒隆态生物工程股份有限公司 | Agricultural straw fast-degradation decomposition agent strain composition |
| CN102174399A (en) * | 2010-12-31 | 2011-09-07 | 广西赛尔纸业有限公司 | Method for preparing herbal biological fiber and biological paper pulp |
Non-Patent Citations (1)
| Title |
|---|
| 水稻根相关细菌的多相分类及Rheinheimera tangshanenesis sp. Nov.的描述;张晓霞;《中国优秀博士学位论文全文数据库 农业科技辑》;20091015(第10期);D043-4 * |
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