CN103054045A - Health-care plant oil composition with blood fat reduction function - Google Patents
Health-care plant oil composition with blood fat reduction function Download PDFInfo
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Abstract
The invention discloses a health-care plant oil composition of herb medicine resource. The health-care plant oil composition comprises the following raw materials in percentage by weight: 40-50% of fructus cannabis oil, 40-50% of linseed oil, 5-6% of walnut oil, 3.5-6% of safflower oil and 0.45-0.55% of vitamin E. The ratio of omega-3 to omega-6 of the health-care plant oil composition is within 1:1-1:4, and animal experiments show that the health-care plant oil composition has good blood fat reduction effects.
Description
Technical field
The invention belongs to the healthy oils field, specifically relate to the health plant fat or oil composition that has hypolipemic function with a kind of.
Background technology
High fat of blood, high cholesterol, hypertension, fatty liver are present epidemic diseases, and reducing blood lipid is hypotensive so become a hot issue.How reducing blood lipid? a lot of people have selected health products, just be popular such as deep sea fish oil, some (comprising some doctors), " worship " its lipid-lowering effect once very all, but deep sea fish oil is very expensive in China.And in daily life, if our daily diet structure of the fine balance of energy just can obtain the effect of good reducing blood lipid, the particularly picked-up of balancing fatty-acid in the vegetable fat that we the most often eat.
According to U.S.'s diet structure analysis result in 1987, the picked-up of ω in the current mankind meals-6 type aliphatic acid and ω-3 type aliphatic acid was unbalanced, and the daily intake of ω-6 is 10-20 times of ω-3.Although ω-6 type aliphatic acid is one of necessary aliphatic acid of human body, but taking in, excessive ω-6 aliphatic acid can play ill-effect to health on the contrary, the Other diseases (as: illnesss such as heart stalk, cerebral infarction, atherosclerotic, diabetes, function of immune system imbalance) that produces high fat of blood, the sticking disease of high blood and derive from thus.
Associating United States Department of Agriculture of the World Health Organization (WHO) (USDA), PAHO (PAHO) and FAO (Food and Agriculture Organization of the United Nation) (FAO) held special meeting in 1999, several great institutions are reached common understanding with regard to the dietary fatty acid ratio, and the recommendation adult absorbs ω-6 type aliphatic acid in daily meals and the optimal ratio of ω-3 type aliphatic acid is between the 2:1-3:1; China then recommends the adult to absorb ω-6 type aliphatic acid in daily meals by " DRIs " the inside of Chinese Soclety of Nutrition's writing and the optimal ratio of ω-3 type aliphatic acid is between the 4:1-6:1.But there are some researches show, the scale dimension of ω in the daily meals-6 type aliphatic acid and ω-3 type aliphatic acid is held in a lower value (in the situation of n-6:n-3≤4:1), can make the incidence of disease of angiocardiopathy and part chronic disease reduce.
At present, the ready-mixed oil of many researchs various reduction ω-6 type aliphatic acid and ω-3 type fatty acid ratio is arranged also, the grease of regulating blood fat is as functional component substantially take Ω-3 composition.The grease in animal class source mainly is fish oil and seal oil, plant origin main take linseed oil, purple perilla wet goods as main, but the prescription ubiquity Ω-3 of these products and the irrational problem of Ω-6 ratio (are taken in for a long time Ω 3 and can be caused level of inflammation rising in the body with the irrational grease of Ω 6 ratios, cause comprising the series of problems of cardiovascular disease), these single greases and simple composite can not addressing this problem.
For example Chinese patent application 201010173183.9, this health-care oil is to be mixed by fish oil and vegetable oil, and described vegetable oil is one or more in sunflower oil, olive oil, sesame oil, cottonseed oil, coconut oil, tea-seed oil, rice bran oil, linseed oil, safflower oil, walnut oil, grape-kernel oil and the pumpkin seed oil.This health-care oil mainly relies on DHA contained in the fish oil (DHA) and EPA (EPA), can improve cranial capacity, strengthens memory and comprehension.
201110169851.5 disclose a kind of family balance formula oil, by camellia seed oil, corn oil, grape-kernel oil, linseed oil 15%-30%, walnut oil, mixed tocopherol 0.04-0.2%.This family's balance formula oil is cooked good stability, is had enhancing development effect and auxiliary antilipemic effect.But find through research, its auxiliary antilipemic effect still has to be strengthened.
Fructus cannabis is one of integration of drinking and medicinal herbs kind of Ministry of Public Health's approval, and use among the people is with a long history.The Compendium of Material Medica record, fructus cannabis has the effect of tonifying middle-Jiao and Qi.From fructus cannabis, extract the fiery sesame oil that obtains and be called as " long life oil ".Fire sesame oil has without cholesterol, essential fatty acid content high, particularly have ω-6 family's linoleic acid and ω-3 family alpha-linolenic acid and be about the ratio of 2.4:1, meeting the characteristics less than or equal to the 4:1 optimum ratio that WHO is recommended, is one of grease of tool nutrient balance.The health that fire sesame oil effectively replenishes needed by human nutrient alpha-linolenic acid, moisturize laxation, the useful heart, brain and the blood vessel effects such as also having the blood fat and blood pressure of reduction blood sugar of promoting longevity.
Chinese patent application 200810235299.3 discloses a kind of ready-mixed oil, and it is comprised of 8-85% cannabis oil and other edible vegetable oil of 15-92%; Wherein, edible vegetable oil is safflower seed oil, corn oil, flax wet goods one or more formations in totally 13 kinds; The weight ratio of n-6 and n-3 is 4-6:1 in this ready-mixed oil, and saturated fatty acid is no more than 15%.Its balanced fatty acid is still reasonable not, and the hypolipemic function of the ready-mixed oil of its disclosed concrete composition is also good not.
Although, the compound grease that keeps healthy all is that the grease interworking by those common plant sources forms mostly, but, different combinations, the performance of the product that obtains but differs greatly, respectively lay particular stress on, according to the dietary requirement of present China, the compound grease of health care with good accent blood fat effect still has multiple product to be developed.
Summary of the invention
Based on this, the present invention proposes a kind of aliphatic acid and forms rationally the health plant fat or oil composition with good accent blood fat effect.
The technical scheme that realizes above-mentioned purpose is as follows:
A kind of health plant fat or oil composition, its raw material by following percentage by weight forms:
Fructus cannabis oil: 40-50%, linseed oil: 40-50%, walnut oil: 5-6%, safflower seed oil: 3.5-6%, vitamin E: 0.45-0.55%.
Preferably, described health plant fat or oil composition is comprised of the raw material of following percentage by weight: fructus cannabis oil: 43-48%, and linseed oil: 42-46%, walnut oil: 5-6%, safflower seed oil: 4.5-5.5%,
Vitamin E: 0.45-0.55%.
Preferably, described health plant fat or oil composition, its raw material by following percentage by weight forms: fructus cannabis oil: 45%, linseed oil: 44%, walnut oil: 5.5%, safflower seed oil: 5%, vitamin E: 0.5%.
Preferably, the formulation of described health plant fat or oil composition is soft capsule or capsule.
Health plant fat or oil composition of the present invention is by inventor's experience and a large amount of experiment screenings, from numerous vegetable fat, through meticulously selecting, obtained good combination from raw material and proportion of composing aspect, accurately control the ratio of Ω-3 and Ω-6, reach the most suitable human body between 1:1-1:4, and have good reduction blood fat effect through the animal experiment checking, for Antilipemic health provides more choices.
Figure of description
Fig. 1 HMR soft capsule is on the impact of rat hyperlipoidemia TC level;
Fig. 2 HMR soft capsule is on the impact of rat hyperlipoidemia TG level;
Fig. 3 HMR soft capsule is on the impact of rat hyperlipoidemia HDL-C level;
Fig. 4 HMR soft capsule is on the impact of rat hyperlipoidemia LDL-C level;
Fig. 5 HMR soft capsule is on the impact of disorders of lipid metabolism rat LDL-C/HDL-C ratio;
Fig. 6 HMR soft capsule is on the impact of disorders of lipid metabolism rat LDL-C/TC ratio.
The specific embodiment
Below by specific embodiment, the present invention is further elaborated.
Embodiment 1:
The described health plant fat or oil composition of the present embodiment, its raw material by following percentage by weight forms: fructus cannabis oil: 45%, linseed oil: 44%, walnut oil: 5.5%, safflower seed oil: 5%, vitamin E: 0.5%.
With above-mentioned raw materials, according to the soft capsule technology of preparing, make Ω 3 through batch mixing, change glue, pelleting, rotating cage setting, drying, technology of the package: Ω 6=1:1 soft capsule.
Embodiment 2
The described health plant fat or oil composition of the present embodiment, its raw material by following percentage by weight forms: fructus cannabis oil: 50%, linseed oil: 40%, walnut oil: 6%, safflower seed oil: 3.5%, vitamin E: 0.5%.
With above-mentioned raw materials, according to the soft capsule technology of preparing, make Ω 3 through batch mixing, change glue, pelleting, rotating cage setting, drying, technology of the package: Ω 6=1:3 soft capsule.
Embodiment 3
The described health plant fat or oil composition of the present embodiment, its raw material by following percentage by weight forms: fructus cannabis oil: 40%, linseed oil: 49%, walnut oil: 5%, safflower seed oil: 5.5%, vitamin E: 0.5%.
With above-mentioned raw materials, according to the soft capsule technology of preparing, make Ω 3 through batch mixing, change glue, pelleting, rotating cage setting, drying, technology of the package: Ω 6=1:4 soft capsule.
Embodiment 4
1 experiment purpose
Set up bait rat hyperlipoidemia, the soft capsule (being called for short the HMR soft capsule) that the raw material of observation embodiment 1 described health plant fat or oil composition is made is to the accent blood fat effect of rat experiment hyperlipoidemia.
2 trial
Carry out " testing and calibration laboratories capability Approval criterion " that CNAS promulgates in January, 2007 and in the microorganism detection field, the application note in field of chemical detection, animal quarantine field.
3 test samples
3.1 with the raw material of the compound grease of embodiment 1 described health care, according to prior art, make soft capsule.
4 positive reference substances
Title (or code name): fenofibrate (French Li Bofuni drugmaker, lot number 93640), seal oil (providing lot number 11F18A01 without thresold China Co., Ltd).
5 animals used as test
5.1 strain and rank: SD rat, SPF level;
5.2 quantity and sex: 80 (mixed type model experiment), male, animal used as test production licence number SCXK (Guangdong) 2008-0002, Quality of Experimental Animals quality certification № 0110204;
5.3 body weight during the experiment beginning: the model experiment of 180-220g(mixed type);
5.4 the unit of providing: provided by Guangdong Medical Lab Animal Center;
5.5 recognition methods: the SD rat is adopted by the wool dyeing method, uses saturated picric acid that cavy is numbered, rat body surface different parts by the hair be coated with the specking point, to show jack per station.With rat skin dyeing and the identification of cage tool dualism number mark;
5.6 feeding and management:
The SD rat feeding is at the SPF of Academy of Traditional Chinese Medicine, Guangdong Province level Animal Lab., animal used as test occupancy permit number: SYXK(Guangdong) 2010-0059, annual test is all qualified.The animal feeding condition: 5/case, group support, raising temperature and humidity: 20~25 ℃, 40~70%, adopt 10h:14h to be interrupted round the clock illumination; The receptacle condition remains stable, with guarantee test result's reliability.Blank group rat feeds with full-valence pellet feed (being provided by Guangdong Medical Lab Animal Center), and the hyperlipidemia model rat feeds with high lipid food (by Guangdong Medical Lab Animal Center's preparation).Ad lib drinking-water, drinking water is freely absorbed by drinking bottle for the pure water that the second Drug Manufacturing Room of institute of traditional Chinese medicine of Guangdong Province provide;
5.7 quarantine: to the SPF level SD rat of the buying 7d that quarantines.The daily check animal once as finds that unsound animal rejects immediately during this time, selects healthy animal to test.
6 main laboratory apparatus and reagent
6.1 main laboratory apparatus: JJ3000 animal electronic scale, G﹠amp; G company product; BS224S electronic balance (1/0,000), German SARTORIUS product; HH-6 digital display thermostat water bath, Jintan City's Fuhua Instr Ltd. product; The 3K30 centrifuge, SIGMA company product; 752N type ultraviolet specrophotometer, Shanghai Precision Scientific Apparatus Co., Ltd's product;
6.2 main agents: Cholesterol Kit, glad biotechnology research institute of Shanghai section, lot number 20120612; The Triglyceride Reagent box, glad biotechnology research institute of Shanghai section, lot number 20120612; Direct HDL-C kit, Zhongsheng Beikong Biological Science ﹠ Technology Co., Ltd., lot number 120451; Direct LDL-C kit, Zhongsheng Beikong Biological Science ﹠ Technology Co., Ltd., lot number 120451;
6.3 keep feed and model feed: provided by Guangdong Medical Lab Animal Center;
6.4 model feed: in keeping feed, add 20.0% sucrose, 15% lard, 1.2% cholesterol, 0.2% sodium taurocholate, an amount of casein, calcium monohydrogen phosphate, stone flour etc.Except crude fat, the moisture of model feed, crude protein, crude fat, crude fibre, coarse ash, calcium, phosphorus, calcium: phosphorus all reaches the national standard of keeping feed.
The design of 7 dosage and grouping
7.1 design considerations: Ministry of Health of the People's Republic of China 2010 editions " health food check and assessment technique standard ".
7.2 dosage design and grouping:
Modeling success SPF level SD rat is carried out random packet (seeing Table 1) according to serum total cholesterol (TC) level, and 10/group, concrete dosage sees Table 1.
Table 1 given the test agent group test plan table
8 experimental techniques
8.1HMR soft capsule is on the impact experiment of disorders of lipid metabolism mixed type model
8.1.1 the laundering period
SPF level SD rat, 80, male, 180-220g feeds to raise and keeps feed, freely drinks water, and 7d is observed in quarantine under barrier system.
8.1.2 the modeling phase
Eyeground vein clump blood sampling after quarantine finishes, the level of TC, TG, HDL-C, LDL-C in the mensuration serum.
Begin 80 rats from formal test and divide 2 groups at random by body weight, 10 rats are kept feed as the blank group, and 70 rats give the model feed as model control group.Weigh in weekly 1 time.The model control group rat gives the model feed after 2 weeks, and blank group and the blood sampling of the equal non-fasting eyeground vein of model control group rat clump are measured serum TC, TG, HDL-C, LDL-C level, to determine whether to form hyperlipemia model.
According to the TC level model control group rat is divided into 7 groups at random, 10/group, i.e. model control group, fenofibrate group, seal oil group, extremely low, the basic, normal, high dosage group of HMR soft capsule.
8.1.3 given the test agent gives
After the grouping, each administration group is gastric infusion 30 days according to dosage, and blank group and model control group give simultaneously with volume distilled water; The blank group continues to keep feed, and model control group and each administration group continue to give the model feed, and regularly weigh in, non-fasting blood sampling when experiment finishes, and separation of serum is measured serum TC, TG, HDL-C, LDL-C level.
8.1.4 detection index
Serum TC, TG, HDL-C, LDL-C level.
9 data are processed and the result judges
9.1 respectively organize experimental data all with number plus-minus standard deviation
Represent, adopt variance analysis, but need to carry out first homogeneity test of variance by the program of variance analysis, variance is neat, calculates the F value, 36F value<F0.05, and conclusion: each organizes no significant difference between mean; F value 〉=F0.05, P≤0.05 is added up with the in twos comparative approach of mean between a plurality of experimental group and control group; The data of abnormal or heterogeneity of variance are carried out suitable variable conversion, after satisfying the neat requirement of normal state or variance, add up with the data after the conversion; If do not reach yet normal state or the neat purpose of variance after the variable conversion, use rank test instead and add up.
9.2 results of animal is judged:
Blood-fat-decreasing function result judges: model control group and blank group compare, and serum triglyceride raises, and serum total cholesterol or LDL-C raise, and difference all has conspicuousness, and decision model is set up.
(1) each dosage group and model control group are relatively, arbitrary dosage group serum total cholesterol or LDL-C reduce, and arbitrary dosage group serum triglyceride reduces, difference all has conspicuousness, each dosage group serum High Density Lipoprotein Cholesterol significantly is not lower than model control group simultaneously, can judge that this given the test agent blood-fat-decreasing function results of animal is positive.
(2) each dosage group and model control group are relatively, arbitrary dosage group serum total cholesterol or LDL-C reduce, difference all has conspicuousness, each dosage group serum triglyceride is not significantly higher than model control group simultaneously, each dosage group serum High Density Lipoprotein Cholesterol significantly is not lower than model control group, can judge the auxiliary serum cholesterol function results of animal positive that reduces of this given the test agent.
(3) each dosage group and model control group are relatively, arbitrary dosage group serum triglyceride reduces, difference all has conspicuousness, each dosage group serum total cholesterol and LDL-C are not significantly higher than model control group simultaneously, serum High Density Lipoprotein Cholesterol significantly is not lower than model control group, can judge the auxiliary serum triglyceride function results of animal positive that reduces of this given the test agent.
10 experimental results
10.1 TC, TG, HDL-C, LDL-C level in the rat blood serum before on-test
Table 2 shows, respectively organizes TC in the rat blood serum, TG, HDL-C before on-test, the LDL-C level is all basically identical, more equal no significant difference between each group (P〉0.05).
Annotate: compare * P<0.05, * * P<0.01 with the blank group; Compare #P<0.05, ##P<0.01 with model control group.
10.2 respectively organize TC in the rat blood serum, TG, HDL-C, LDL-C level before the administration
Table 3 shows, compares with the blank group, and all the other respectively organize TC in the rat blood serum, TG, the LDL-C level significantly raises (P<0.01), and the HDL-C level obviously reduces (P<0.01).Prompting hyperlipidemia rats hyperlipoidemia is set up.
Except the blank group, mutually compare between extremely low, basic, normal, high each group of fenofibrate, seal oil, HMR soft capsule, TC, TG, HDL-C, the equal no significant difference of LDL-C level (P〉0.05).
Respectively organize before table 3 administration TC in the rat blood serum, TG, HDL-C, LDL-C level (
N=10)
Annotate: compare * P<0.05, * * P<0.01 with the blank group; Compare #P<0.05, ##P<0.01 with model control group.
10.3HMR soft capsule is on the impact of TC, TG, HDL-C, LDL-C level in the disorders of lipid metabolism rat blood serum
Table 4 shows, compare with the blank group, TC, TG, LDL-C level significantly raise (P<0.01) in the model control group rat blood serum, and the HDL-C level has to a certain degree reduction, and prompting keeps stable at 30 days administration after date hyperlipidemia rats hyperlipoidemias.
Table 4 and Fig. 1~Fig. 4 shows, compare with model control group, TC, TG, LDL-C level obviously reduce (P<0.01 or P<0.05) in extremely low, the basic, normal, high dosage group of the HMR soft capsule rat blood serum, HDL-C level significantly raise (P<0.01 or P<0.05) in the middle and high dosage group of the HMR soft capsule rat blood serum.The auxiliary blood fat function results of animal of transferring of prompting HMR soft capsule is positive.
With the fenofibrate group relatively, the HMR soft capsule is extremely low, low dose group HDL-C level obviously reduces (P<0.05), all the other each groups respectively detect index there are no significant difference (P〉0.05).
With the seal oil group relatively, TC, TG, LDL-C level there are no significant difference in extremely low, the basic, normal, high dosage group of the HMR soft capsule rat blood serum (P〉0.05).
Mutually compare between extremely low, the basic, normal, high dosage group of HMR soft capsule, HMR soft capsule high dose group TC level reduces (P<0.01) than the horizontal conspicuousness of HMR soft capsule very low dose group TC, though all the other each groups respectively detect index value there are no significant difference not of uniform size (P〉0.05).
Table 4HMR soft capsule on the impact of TC, TG, HDL-C, LDL-C level in the disorders of lipid metabolism rat blood serum (
N=10)
Annotate: compare * P<0.05, * * P<0.01 with the blank group; Compare #P<0.05 with model control group,
##P<0.01; Compare △ P<0.05, △ △ P<0.01 with the fenofibrate group; Compare ◇ P<0.05 with the seal oil group,
◇ ◇ P<0.01; Compare ☆ P<0.05, ☆ ☆ P<0.01 with the extremely low group of HMR soft capsule.
10.4HMR soft capsule is on the impact of disorders of lipid metabolism rat LDL-C/HDL-C ratio, LDL-C/TC ratio
Table 5 shows, with the blank group relatively, model control group rat LDL-C/HDL-C ratio significantly raises (P<0.01), LDL-C/TC ratio decreases but there was no significant difference (P〉0.05).
Compare with model control group, extremely low, the basic, normal, high dosage group of HMR soft capsule rat LDL-C/HDL-C ratio obviously reduces (P<0.01), difference that LDL-C/TC ratio raises to some extent but there are no significant (P〉0.05).
Compare with the fenofibrate group, extremely low, the basic, normal, high dosage group of HMR soft capsule rat LDL-C/HDL-C ratio there was no significant difference (P〉0.05), LDL-C/TC ratio there was no significant difference (P〉0.05).
Compare with the seal oil group, extremely low, the basic, normal, high dosage group of HMR soft capsule rat LDL-C/HDL-C ratio there was no significant difference (P〉0.05), LDL-C/TC ratio there was no significant difference (P〉0.05).
Mutually compare between extremely low, the basic, normal, high dosage group of HMR soft capsule, LDL-C/HDL-C ratio, LDL-C/TC ratio there are no significant difference (P〉0.05).
Fig. 5, Fig. 6 demonstration, extremely low, the basic, normal, high dosage group of HMR soft capsule rat LDL-C/HDL-C ratio increases on a declining curve with dosage, and LDL-C/TC ratio increases in rising trend with dosage.
Table 5HMR soft capsule on the impact of disorders of lipid metabolism rat LDL-C/HDL-C ratio, LDL-C/TC ratio (
N=10)
Annotate: compare * P<0.05, * * P<0.01 with the blank group; Compare #P<0.05, ##P<0.01 with model control group; Compare △ P<0.05, △ △ P<0.01 with the fenofibrate group; Compare ◇ P<0.05, ◇ ◇ P<0.01 with the seal oil group; Compare ☆ P<0.05, ☆ ☆ P<0.01 with the extremely low group of HMR soft capsule.
Model control group relatively, TC, TG, LDL-C level obviously reduce (P<0.01 or P<0.05) in extremely low, the basic, normal, high dosage group of the HMR soft capsule rat blood serum, HDL-C level significantly raise (P<0.01 or P<0.05) in the middle and high dosage group of the HMR soft capsule rat blood serum.Health plant fat or oil composition of the present invention has good accent blood fat effect.
The above embodiment has only expressed several embodiment of the present invention, and it describes comparatively concrete and detailed, but can not therefore be interpreted as the restriction to claim of the present invention.Should be pointed out that for the person of ordinary skill of the art, without departing from the inventive concept of the premise, can also make some distortion and improvement, these all belong to protection scope of the present invention.Therefore, the protection domain of patent of the present invention should be as the criterion with claims.
Claims (4)
1. a health plant fat or oil composition is characterized in that, its raw material by following percentage by weight forms:
Fructus cannabis oil: 40-50%, linseed oil: 40-50%, walnut oil: 5-6%, safflower seed oil: 3.5-6%, vitamin E: 0.45-0.55%.
2. health plant fat or oil composition according to claim 1 is characterized in that, its raw material by following percentage by weight forms: fructus cannabis oil: 43-48%, linseed oil: 42-46%, walnut oil: 5-6%, safflower seed oil: 4.5-5.5%, vitamin E: 0.45-0.55%.
3. health plant fat or oil composition according to claim 1 is characterized in that, its raw material by following percentage by weight forms: fructus cannabis oil: 45%, and linseed oil: 44%, walnut oil: 5.5%, safflower seed oil: 5%, vitamin E: 0.5%.
4. each described health plant fat or oil composition is characterized in that according to claim 1-3, and the formulation of described health plant fat or oil composition is soft capsule or capsule.
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CN109007840A (en) * | 2018-09-18 | 2018-12-18 | 广东雪芝康生物科技有限公司 | A kind of nutritive plant powder and preparation method thereof by adjusting gene reducing weight and blood fat |
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