CN103053808A - Silage additive and preparation method and application thereof - Google Patents
Silage additive and preparation method and application thereof Download PDFInfo
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Abstract
The invention discloses a silage additive and a preparation method and application of the silage additive. The active ingredient of the silage additive is a composition of (a) or (b) as follows: a composition consisting of an extract of radix astragali and an extract of fructus crataegi; and the two extracts are mixed together or separately packed. The prepared silage has at least one of the following characteristics: (1) the content of crude protein is higher than that in the control group; (2) the ratio of ammoniacal nitrogen to silage additive is lower than that in the control group; (3) the content of neutral detergent fiber is lower than that in the control group; (4) the content of acid detergent fiber is lower than that in the control group; (5) the pH value is lower than that in the control group; and (6) the content of lactic acid is higher than that in the control group, and the content of butyric acid is lower than that in the control group.
Description
Technical field
The present invention relates to a kind of Chinese herbal medicine type ensilage bio-additive and application, particularly a kind of silage additive and preparation method thereof and application.
Background technology
Ensilage directly utilized or made hay than similar greenfeed digestibility is high, and digestible crude protein matter, total nutrient and energy content in the ensilage dry are also higher, also can obviously improve through ensilage cell wall constituent digestibility behind the silage fermentation.But the buffer value of greenfeed is high under the nature, and water-soluble carbohydrate content is low, ensiling difficulty relatively under the nature, and this phenomenon is particularly evident in leguminous forage.Additives for ensiling can be accelerated silage fermentation speed, improves the ensilage quality.In recent years, the kind of additives for ensiling both domestic and external mainly contains ammonium compounds, organic acid, urea and biological preservative etc., but inorganic chemistry class additive has certain corrosivity, and there are potential safety hazard in people and domestic animal, and the biological species additive is many from external import, and cost is higher.
At present, it is more common to add antibiotic, hormone, chemical synthetic drug in the prevention of livestock and poultry and treatment in the feed, although its successful, but usually in product, have residual, the harm consumer health, it is serious three to cause (carcinogenic, teratogenesis, mutagenesis) phenomenon, easily develops immunity to drugs simultaneously.Efficient in order to obtain, safe, non-harmful livestock products add Chinese herbal medicine and become a kind of new trend in animal and fowl fodder.But in producing both at home and abroad at present; mix rear Direct-fed mainly with Chinese medicine meal and feed; this uses very inconvenient in the situation of intensive culture; thereby greatly having limited Chinese herbal medicine uses in the scale that prevents and treats aspect the Animal diseases; and the annual production of Chinese herbal and crude drugs preparations is also very limited, does not satisfy actual needs at all.Chinese herbal and crude drugs preparations added in the ensiling raw material ferment, when improving Silage Quality, also provide effective way for applying of Chinese herbal medicine.
Summary of the invention
The purpose of this invention is to provide a kind of silage additive and preparation method thereof and application.
Silage additive provided by the invention, its active component be following a) or b) composition:
The composition that is formed by the extract of the extract of the Radix Astragali and hawthorn; Wherein, two kinds of extract mixtures are combined;
The composition that is formed by the extract of the extract of the Radix Astragali and hawthorn; Wherein, two kinds of respectively independent packagings of extract.
Described a) and b) in the composition, the Radix Astragali has another name called yellow over sixty years of age, is the root of leguminous herbaceous plant's astragalus mongolicus, Astragalus membranacus; Hawthorn is the ripening fruits of rosaceous plant large-fruited Chinese hawthorn or fructus crataegi cuneatae.
Described a) and b) in the composition, the mass ratio of the extract of the Radix Astragali and the extract of hawthorn is 0.75-1.25: 0.75-1.25, is specially 0.75: 0.75 or 1: 1 or 1.25: 1.25.
Wherein, the extract of the described Radix Astragali is that water extracts the water-soluble substances that obtains from the Radix Astragali; Concrete, the extract of the described Radix Astragali gets according to the method preparation that comprises the steps: boil after the Radix Astragali is soaked 30min, carrying out the first time after the slow fire boiling filters, obtain for the first time filtrate and filter residue, after adding behind the water 150ml again slow fire boiling in the gained filter residue, carry out the second time and filter, with gained for the second time filtrate and for the first time filtrate merge, it is 0.96-1.04g/ml that slow fire boiling is concentrated into solid content, obtains the extract of the described Radix Astragali;
The extract of described hawthorn is that water extracts the water-soluble substances that obtains from hawthorn; Concrete, the extract of described hawthorn gets according to the method preparation that comprises the steps: boil after hawthorn is soaked 30min, carrying out the first time after the slow fire boiling filters, obtain for the first time filtrate and filter residue, after adding behind the water 150ml again slow fire boiling in the gained filter residue, carry out the second time and filter, with gained for the second time filtrate and for the first time filtrate merge, it is 0.96-1.04g/ml that slow fire boiling is concentrated into solid content, obtains the extract of described hawthorn;
The amount ratio of the described Radix Astragali or hawthorn and immersion water specifically is 24.5-25.5g: 249-251mL, more specifically is 25g: 250mL;
In the described decoction step, the slow fire boiling time is specially 25-35 minute, more specifically is 30 minutes;
In the described filtration step, the filter cloth aperture is specially 0.9mm-1.1mm, more specifically is 1mm;
Described solid content all is specially 1g/mL.
Described silage additive is specially ensilage fermentation promoter.Described ensilage fermentation promoter is a kind of the adding before fermentation in the ensiling raw material, thereby carries out the silage additive that anaerobic fermentation promotes fermentation with described ensiling raw material.
The method of the described silage additive of preparation provided by the invention comprises the steps: the extract of the aforementioned Radix Astragali and the extract of hawthorn are mixed or independent packaging, obtains the active component of described silage additive.
The method for preparing ensilage provided by the invention is carried out anaerobic fermentation after comprising the steps: to add aforementioned silage additive in the ensiling raw material, obtains described ensilage.
In the said method, the ensiling raw material is for making the raw material of ensilage, be greenfeed (also being green forage, greenfeed material), refer to and as the fresh cauline leaf of plant of feed, mainly to comprise native grass, tame forage grass, weeds in field, dish leaf class, water plant, spray leaf etc.
Described ensilage is the feed that greenfeed is fermented and obtains under the condition of anaerobism.
The water content of described ensiling raw material is 60%-75%, is specially 60% or 65% or 70% or 60-70% or 65-70%;
The addition of described silage additive with described a) or b) dry weight basis of composition, the 0.75%-1.25% of described ensiling material quality, be specially water content and be 60%-75% (60% or 65% 70% or 60-70% or 65-70%) 0.75%-1.25% of quality of ensiling raw material.
In the described anaerobic fermentation step, temperature is 15-30 ℃, and the time is 30-90 days, is specially 45 days.
Ensilage according to preceding method prepares also belongs to protection scope of the present invention.Wherein, described ensilage has following at least a characteristic:
1) crude protein content is higher than contrast;
2) ratio of ammoniacal nitrogen and total nitrogen is lower than contrast;
3) neutral detergent fiber content is lower than contrast;
4) acid detergent fiber content is lower than contrast;
5) the pH value is lower than contrast;
6) lactic acid is higher than contrast, and butyric acid is lower than contrast.
Described contrast is the feed through following method preparation: except not adding described silage additive, other condition is all identical with preceding method.
Described ensilage is the leguminous forage ensilage, is specially clover and belongs to ensilage, more specifically the ensilage for preparing take alfalfa as the ensiling raw material;
Described ensiling raw material is leguminous forage, is specially clover and belongs to herbage, more specifically is alfalfa (Medicago sativa).
The present invention will be fermented as silage additive and ensiling raw material jointly by the mixture that the Radix Astragali and hawthorn form, can promote the fermentation quality of lactobacter growth breeding and raising ensilage, prepared ensilage, compare with the contrast that does not add described silage additive, its crude protein content raises, and/or the reduction of the ratio of ammoniacal nitrogen and total nitrogen, and/or the neutral detergent fiber content, and/or the acid detergent fiber content, and/or the pH value reduces.
The specific embodiment
The present invention is further elaborated below in conjunction with specific embodiment, but the present invention is not limited to following examples.Described method is conventional method if no special instructions.Described raw material all can get from open commercial sources if no special instructions.The below take alfalfa (Medicago sativa) as the ensiling raw material as example, illustrate technical scheme of the present invention.
The preparation of embodiment 1, silage additive
Take by weighing Radix Astragali 25g, the 250mL deionized water decocts after soaking 30min, the rear slow fire boiling 30min that boils is that 4 layers of gauze of 1mm carry out filtering and obtain for the first time filtrate and the dregs of a decoction first time with the aperture, adds 150ml water in the dregs of a decoction, carrying out the second time behind the slow fire boiling 30min filters, after the gained filtrate second time and for the first time filtrate merging, slow fire boiling is concentrated into 25ml, and making its solid content is 1g/ml, be 100% Chinese herbal medicine stoste, this 100% Chinese medicine stoste is the extract of the Radix Astragali.
According to upper identical method, the Radix Astragali is replaced with hawthorn, obtain the extract of hawthorn.
The extract of the above-mentioned Radix Astragali and the extract of hawthorn were mixed with mass ratio respectively in 0.75: 0.75,1: 1 and 1.25: 1.25, obtain successively silage additive 1)-3).
Embodiment 2, prepare the Medicago Sativa Silage feed with the silage additive of embodiment 1
One, material
Ensiling raw material: get the bright grass of alfalfa (Medicago sativa) that initial bloom stage cradles and carry out airing, carry out during this time multi-point sampling of many time, measure water content with the micro-wave oven rapid dry process, when its moisture reaches 75% respectively, be cut to 2-3cm with chopper, obtain the ensiling raw material.
Two, experimental technique
The ensiling raw material is divided into four groups, comprises silage additive 1-3 test group and control group.
Wherein, 1 group of silage additive 1 of pressing the embodiment 1 of the 15g that adds dry weight basis in the bright grass of 1kg of silage additive);
2 groups of silage additives 2 of pressing the embodiment 1 of the 20g that adds dry weight basis in the bright grass of 1kg of silage additive);
3 groups of silage additives are pressed the silage additive 3 among the embodiment 1 of the 25g that adds dry weight basis in the bright grass of 1kg);
Control group does not add any material.
The liquid of as stated above preparation evenly is sprayed on the alfalfa, brighter grass is put in the Polypropylene Bag, about every bag of 200g, with the vacuum sealer sealing, (25-38 ℃) carries out silage fermentation at ambient temperature, opens bag after 45 days.In air, expose sampling in the 1st, 3,5,7 day ensiling 45 days and after opening bag, carry out lab analysis, Main Analysis pH value, dry, crude protein, ammoniacal nitrogen, neutral detergent fiber, acid detergent fiber are opened a bag rear mouldy degree, and experimental result sees Table 1, table 2 and table 3.
The assay method of each index of reaction ensilage quality is as follows:
(1) dry is measured:
Measure with the oven for drying method: get the bright sample of ensiling raw material or ensilage and weigh and write down weight, 65 ℃ of dry 48h in baking oven are until constant weight, and record alleged weight to calculate dry (DM) content.
(2) pH pH-value determination pH:
Accurately take by weighing the ensiling 10.0g that shreds, add the 90mL distilled water rear usefulness that stirs and organize pulper to stir 1 minute.Qualitative filter paper and four layers of filtered through gauze are with the pH value of pH analyzer mensuration filtrate.
(3) mensuration of ammoniacal nitrogen/total nitrogen value (Broderick and Kang, 1980) adopts phenol-clorox colorimetric method for determining:
Phenol reagent: with 0.05g sodium nitroprusside (Na
2Fe (CN)
5NO2H
2O, 297.95, chemical pure (25g)) be dissolved in the 0.5L distilled water, add again 11mL phenol solution or 9.9g crystallization phenol (C
6H
5O, 94.11, Phenol A.R., 500g analyzes pure), constant volume is stored in the brown glass reagent bottle behind 1L.
Clorox reagent: 5gNaOH is dissolved in the distilled water of 2/3L, adds again 20.1gNa
2HPO
4Be heated to dissolving.Add 14.7ml after the cooling and contain liquor natrii hypochloritis (74.44, analyzes purely, Active Chlorine does not hang down 7%) and the mixing of 8.5% Active Chlorine, constant volume is to 1L.
Standard ammonia solution: take by weighing 0.6607g through the (NH of 100 ℃ of oven dry in 24 hours
4)
2SO
4(132.14, chemical pure) are dissolved in the distilled water, and are settled to 100ml, are mixed with the ammonia storing solution of 100mmol/L, above-mentioned storing solution dilution are mixed with the titer of five kinds of variable concentrations gradients of 1.0,2.0,3.0,4.0,5.0mmol/L.
The brief operation step: add 50ul through sample liquid or the titer of suitable multiple dilution in every test tube, blank is 50ul distilled water; Add the phenol reagent of 2.5ml in every test tube, shake up; In every test tube, add 2ml clorox reagent again, and mixing; Mixed liquor was heated chromogenic reaction 5 minutes in 95 ℃ of water-baths; After the cooling, colorimetric under the 630nm wavelength.
(4) acid detergent fiber (ADF), neutral detergent fiber (NDF) are measured and are adopted Fan Shi washing the fibre method to measure;
The preparation of NDF medicine (a Fibre Bag 0.5g sample): take by weighing 1.86g disodium ethylene diamine tetraacetate (EDTA, 372.24), 0.68g Boratex (Na
2B
4O
710H
2O, chemical pure 381.37), 3g lauryl sodium sulfate (chemical pure 288.38), 1ml ethylene glycol ethyl ether (chemical pure 90.12); 0.456g ADSP (chemical pure 141.96), the 0.5g anhydrous sodium sulfite.Dissolve in the 100ml distilled water.
The brief operation step: after the weight peeling of weighing filter bag, weighing 0.4500-0.5500g sample is put into filter bag, seals at distance sack 4mm place with sealing machine.Accurate blank filter bag of weighing, filter bag is placed on the filter bag frame, put into the fibre analysis instrument, put into weight and guarantee that the superiors' filter bag can be immersed under the liquid level, before beginning, to the Alpha-amylase of adding 4.0ml in the fiber instrument, put into amylase liquid feeding (the Alpha-amylase of 8.0ml is diluted to 250ml) at B mouth place.The fiber instrument washed, and filter bag is taken out, and at 105 ℃ of bone dry 2-4 hours, cooling was weighed.
The preparation of ADF medicine: take by weighing 20g hexadecane trimethyl ammonia bromide (CTAB, chemical pure 364.47) and be dissolved in (0.5mol/L sulfuric acid: get 55.74ml concentrated sulfuric acid adding distilled water and be settled to 1L) in the 1000ml0.5mol/L sulfuric acid solution.
The brief operation step: after the weight peeling of weighing filter bag, weighing 0.4500-0.5500g sample is put into filter bag, seals at distance sack 4mm place with sealing machine.Accurately blank filter bag of weighing is placed on filter bag on the filter bag frame, puts into the fibre analysis instrument, puts into weight and guarantees that the superiors' filter bag can be immersed under the liquid level.The fiber instrument washed, and filter bag is taken out, and at 105 ℃ of bone dry 2-4 hours, cooling was weighed.
(5) mensuration of thick protein: adopt triumphant formula nitriding
1% the BAS that contains bromocresol green and methyl red indicator: with 100g boric acid (Boric cid, 61.83, H
3BO
3, analyze pure, (500g)) be dissolved in 10 liters of distilled water and be made into 1% BAS.Add 100ml bromocresol green (Bromo cresol green, C
21H
14O
5Br
4S, 698.02, (5g)) solution (the 100mg bromocresol green is dissolved in 100ml ethanol).Add 70ml methyl red solution (100mg is dissolved in 100ml ethanol).0.1N HCl:16.6ml hydrochloric acid adds in the 1000ml distilled water.Add again 40%NaOH; Catalyst is K
2SO
4: CuSO
4=15: 1.
Concise and to the point operating procedure: careless sample is dried careless sample pulverized 1mm sieve, about weighing grass sample 5.0g, putting it into disappears boils the catalyst that adds 5.0g in the pipe and the concentrated sulfuric acid of 10.0ml, being placed on disappears boils on the stove, boil about 1 hour in disappearing under 410 ℃, the cooked rear cooling that disappears is measured with triumphant formula azotometer.
(6) organic acid is measured:
Adopt high performance liquid chromatography SHIMADZE-10A to measure lactic acid and volatile fatty acid (acetic acid, propionic acid and butyric acid).Chromatographic column: Shodex Rspak KC-811S-DVB gel Column30 * 8mm; Detector: SPD-M10AVp; Mobile phase: 3mmol/L perchloric acid; Flow velocity: 1ml/min; Column temperature: 50 ℃; Detect wavelength: 210nm; Sample size: 5 μ l.
Concise and to the point operating procedure: accurately take by weighing the ensiling 10.0g that shreds, add the 90mL distilled water rear usefulness that stirs and organize pulper to stir 1 minute.Qualitative filter paper and four layers of filtered through gauze are filtered filtrate with filter again, can be placed under-20 ℃ the condition for subsequent usely, use hplc determination.
(7) observation of mouldy degree:
After ensiling was opened bag in 45 days, observe the mouldy situation of each group, be divided into 0 (having no mildew), 1 (slightly mouldy), 2 (seriously mouldy) by mouldy degree.
The test the data obtained is processed with Excel software, and with the one-way analysis of variance program in the SPSS17.0 software data is carried out variance analysis and multiple ratio.
Three, experimental result
Table 1, silage additive are on the impact (the gained percentage composition is all take dry as the basis in the table) of Alfalfa Silage Fermentation Quality
| Group | The pH value | Ammoniacal nitrogen/total nitrogen (%) | Lactic acid (%) | Butyric acid (%) |
| Contrast | 5.71±0.06a | 18.90±1.61a | 3.66±0.28b | 1.33±0.18a |
| 1 | 4.93±0.02b | 13.40±1.33b | 5.91±0.94a | 0.00±0.00b |
| 2 | 4.85±0.01c | 12.22±0.36b | 5.93±0.50a | 0.00±0.00b |
| 3 | 4.82±0.02c | 11.48±0.58b | 6.24±0.29a | 0.00±0.00b |
Annotate: the different significance degree of The English alphabet differential of each columns value back, have between the processing of at least one same letter in 0.05 level without significant difference, do not have that there were significant differences in 0.05 level between the processing of same letter.
Table 2, silage additive are on the impact of alfalfa ensilage nutritional labeling
| Group | Dry (%) | Neutral detergent fiber (%) | Acid detergent fiber (%) | Crude protein (%) |
| Contrast | 23.94±0.09b | 41.56±0.63a | 31.08±0.28a | 21.70±0.34b |
| 1 | 23.60±0.43b | 41.03±0.78a | 30.26±0.41ab | 23.26±0.75a |
| 2 | 24.93±0.67a | 40.27±0.18a | 28.82±0.31b | 24.16±0.45a |
| 3 | 24.87±0.49a | 40.95±0.30a | 29.98±0.44ab | 23.67±0.21a |
Annotate: the different significance degree of The English alphabet differential of each columns value back, have between the processing of at least one same letter in 0.05 level without significant difference, do not have that there were significant differences in 0.05 level between the processing of same letter.
Table 3, silage additive are on the impact of alfalfa ensilage aerobic stability
Annotate: the different significance degree of The English alphabet differential of each columns value back, have between the processing of at least one same letter in 0.05 level without significant difference, do not have that there were significant differences in 0.05 level between the processing of same letter.
In the table 1, the pH of test group 1-3 significantly is lower than control group, and the ammoniacal nitrogen of test group 1-3 significantly is lower than control group, and the lactic acid content of test group 1-3 is significantly higher than control group, and the butyric acid content of test group 1-3 is 0, significantly is lower than control group.In the ensilage, the breeding of the harmful bacterias such as clostridium, bacillus, Escherichia coli is so that protein and amino acid are decomposed into ammoniacal nitrogen, thereby causes the nutritional labeling of ensilage to run off in a large number, greatly reduces the Feeding Value of ensilage.Clostridium produces butyric acid in the time of fermentation, makes ensilage distribute stench, affects its palatability.Lactic acid bacteria is the main advantage flora in the ensiling, its generation lactic acid that can ferment, and the pH of fast reducing ensilage suppresses the growth of harmful bacteria, thereby reduces the loss of protein, amino acid, dry etc.Therefore, ammoniacal nitrogen, butyric acid content are higher, and Silage Quality is poorer.Lactic acid content is high, and the low explanation of pH value Silage Quality is good.Hence one can see that, and above-mentioned silage additive can effectively improve the fermentation quality of alfalfa ensilage, improves protein utilization.
In the table 2, test group 2,3 dry matter content are significantly higher than control group; The neutral detergent fiber of test group 1-3 is lower than control group, but difference is not remarkable; The acid detergent fiber of test group 1-3 is lower than control group, and wherein test group 2 significantly is lower than control group; The crude protein of test group 1-3 is significantly higher than control group.Plant feed boils processing through neutral detergent, and undissolved residue is neutral detergent fiber, comprises hemicellulose, cellulose, lignin and silicate.After neutral detergent fiber stem acid detergent was processed, residual residue was acid detergent fiber, comprises cellulose, lignin and silicate.Neutral detergent fiber and acid detergent fiber digest slowly or indigestion in animal body, and its too high levels can affect feed intake and the digestibility of animal.Hence one can see that, and above-mentioned silage additive can effectively be preserved the nutritional labeling of ensiling raw material, and the cellulose in the degrading plant cell membrane improves digestibility.
In the table 3, aerobic exposes 1,3,5,7 day test group 2,3 dry matter content is significantly higher than control group; Aerobic exposes the acid detergent fiber of 1,3,5,7 day test group 1-3 and the content of neutral detergent fiber all is lower than control group, and there were significant differences with control group at the 3rd, 5,7 day; The crude protein content that aerobic exposes 1,3,5,7 day test group 1-3 is significantly higher than control group.Hence one can see that, and above-mentioned silage additive can improve the aerobic stability of alfalfa ensilage, prolong its Kaifeng after holding time.
Claims (10)
1. silage additive, its active component be following a) or b) composition:
The composition that is formed by the extract of the extract of the Radix Astragali and hawthorn; Wherein, two kinds of extract mixtures are combined;
The composition that is formed by the extract of the extract of the Radix Astragali and hawthorn; Wherein, two kinds of respectively independent packagings of extract.
2. additive according to claim 1 is characterized in that: described a) and b) in the composition, the mass ratio of the extract of the Radix Astragali and the extract of hawthorn is 0.75-1.25: 0.75-1.25, is specially 0.75: 0.75 or 1: 1 or 1.25: 1.25.
3. additive according to claim 1 and 2, it is characterized in that: the extract of the described Radix Astragali is that water extracts the water-soluble substances that obtains from the Radix Astragali;
The extract of described hawthorn is that water extracts the water-soluble substances that obtains from hawthorn.
4. arbitrary described additive according to claim 1-3, it is characterized in that: described silage additive is ensilage fermentation promoter.
5. method for preparing the arbitrary described silage additive of claim 1-4, comprise the steps: the extract of the arbitrary described Radix Astragali of claim 1-4 and the extract of hawthorn are mixed or independent packaging, obtain the active component of described silage additive.
6. a method for preparing ensilage is carried out anaerobic fermentation after comprising the steps: to add the arbitrary described silage additive of claim 1-4 in the ensiling raw material, obtains described ensilage.
7. method according to claim 6, it is characterized in that: the water content of described ensiling raw material is 60%-75%;
The addition of described silage additive with described a) or b) dry weight basis of composition, be the 0.75%-1.25% of described ensiling material quality; Or,
In the described anaerobic fermentation step, temperature is 15-30 ℃, and the time is 30-90 days, is specially 45 days.
8. the ensilage for preparing of claim 7 or 8 arbitrary described methods.
9. ensilage according to claim 8, it is characterized in that: described ensilage has following at least a characteristic:
1) crude protein content is higher than contrast;
2) ratio of ammoniacal nitrogen and total nitrogen is lower than contrast;
3) neutral detergent fiber content is lower than contrast;
4) acid detergent fiber content is lower than contrast;
5) the pH value is lower than contrast;
6) lactic acid is higher than contrast, and butyric acid is lower than contrast.
Described contrast is the feed through following method preparation: except not adding described silage additive, other condition is all identical with claim 6 or 7 described methods.
10. according to claim 1-4 arbitrary described silage additive, described method, claim 6 or 7 described method, claim 8 or the 9 described silage additives that prepare ensilage that prepare silage additive of claim 5, it is characterized in that: described ensilage is the leguminous forage ensilage, be specially clover and belong to ensilage, more specifically the ensilage for preparing take alfalfa as the ensiling raw material;
Described ensiling raw material is leguminous forage, is specially clover and belongs to herbage, and more specifically be alfalfa.
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| CN103535564A (en) * | 2013-10-21 | 2014-01-29 | 河北省农林科学院农业资源环境研究所 | Method for improving of high-moisture alfalfa ensiling quality by adding feed jujube powder |
| WO2015041556A1 (en) | 2013-09-23 | 2015-03-26 | Ivetić Aleksandra | The method and the use of additives in feed preservation |
| CN106036043A (en) * | 2016-05-26 | 2016-10-26 | 北京助尔生物科学研究院（有限合伙） | Method for improving quality of sorghum hybrid sudangrass silage by utilizing southernwood extracting solution |
| CN106954739A (en) * | 2017-04-07 | 2017-07-18 | 榆林学院 | A kind of processing method of alfalfa silage |
| CN107912605A (en) * | 2017-12-12 | 2018-04-17 | 湖南德人牧业科技有限公司 | A kind of milk cow alfalfa silage and preparation method thereof |
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| CN111690556A (en) * | 2020-05-25 | 2020-09-22 | 河北省农林科学院农业资源环境研究所 | Rapid enrichment agent for lactic acid bacteria on surface of alfalfa plant leaf and use method thereof |
| CN114532445A (en) * | 2022-04-08 | 2022-05-27 | 灵武市同心农业综合开发有限公司 | Additive for pasture silage |
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| CN114532445A (en) * | 2022-04-08 | 2022-05-27 | 灵武市同心农业综合开发有限公司 | Additive for pasture silage |
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