CN103053804B - Farming method capable of improving immunity of live pigs - Google Patents
Farming method capable of improving immunity of live pigs Download PDFInfo
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- CN103053804B CN103053804B CN201210377039.6A CN201210377039A CN103053804B CN 103053804 B CN103053804 B CN 103053804B CN 201210377039 A CN201210377039 A CN 201210377039A CN 103053804 B CN103053804 B CN 103053804B
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Abstract
The invention discloses a farming method capable of improving immunity of live pigs. The method comprises the steps of performing self-help feeding, water-drinking, immunization, disinfection, feeding and management according to conventional ways of a pig farm, and simultaneously adding 0.08% (weight/weight) of traditional Chinese medicine micro-ecological preparation into a basic ration for feeding. According to the farming method disclosed by the invention, the productivity of fattening pigs is significantly promoted, the content of immunoglobulin in serum and the concentration of a complement in the serum are simultaneously increased, and the immune function of the fattening pigs is improved.
Description
Technical field
The invention belongs to livestock and poultry cultivation technical field, in particular to a kind of cultural method that improves live pig immunity.
Background technology
In herding is produced, feeding antibiotic is used oneself to surpass half a century as growth accelerator, its in prevention from suffering from the diseases, improve the aspects such as production performance and have extremely important effect,, feeding environment poor, managerial skills fall behind area large at cultivation density particularly, antibiotic use is more general, and additive capacity is larger.But along with abuse of antibiotics, its negative interaction also manifests thereupon.Antibiotic long-term use causes the immunity degradation of animal, causes the generation of harmful bacteria drug resistance.Meanwhile antibiotic is residual in livestock products, also serious threat is to the mankind's health, increasing researcher thinks, the raising of mankind's certain cancers and the lopsided incidence of disease, drug-fast generation and some food poisoning phenomenon is residual relevant with antibiotic, hormone and some other additive in livestock products all.
These problems form potential threat to human health, are more and more subject to people's attention.Along with improving constantly of people's Consciousness of food security, people use Antibiotic Additive for standard, or even the hope of forbidding Antibiotic Additive is more and more stronger.In developed countries such as European Union, Japan, in succession promulgated and forbidden and limit and use the application measure of Antibiotic Additive in feed.World Health Organization has sent warning in the special meeting in October, 1997: the antibiotic property system that comprises four kinds of bacteriums of salmonella has passed to the mankind from animal, the mankind are being faced with not and can, to the antibiotic aitiogenic risk of infection of curing the disease, appealing livestock and poultry to be cautious use of antibiotic.Therefore, very pay attention to the research of natural disease-resistant substance and growth promoter class feed addictive both at home and abroad, finding non-harmful Substitutes For Antibiotic has become the problem that countries in the world are competitively studied.
In order to adapt to the trend requirement of feed addictive future development, adopt environmental type feed addictive to replace antibiotic to carry out livestock and poultry cultivation, thereby improve immunity of livestock, this seems more and more urgent.
Summary of the invention
Order of the present invention is, by various green feed additives are studied, to provide a kind of method that adopts Chinese medicine microecological preparation to carry out pig-breeding.
The object of the present invention is achieved like this:
Improve a cultural method for live pig immunity, the method is included in adds 0.05%-0.1%(w/w on basal diet) Chinese medicine microecological preparation fed; Described Chinese medicine microecological preparation is prepared as follows and is obtained:
(1) take clean and dry dried orange peel 10-18 part, root of herbaceous peony 10-18 part, cordate houttuynia 8-14 part, rhizoma atractylodis 6-10 part, Radix Astragali 6-10 part, be ground into particle, add for the first time the 8-12 times of water gaging that mixes medicinal material gross weight, decoct 2 hours, add for the second time the 6-8 times of water gaging that mixes medicinal material gross weight, decoct collecting decoction 1 hour, be down to room temperature, standby;
(2) press the CFU ratio of 1-5:1, get synanthrin lactobacillus (
sporolactobacillus inulinus) CGMCC No.2185, Rhizopus oryzae (
rhizopus oryzae) CCTCC No.M209191 bacterium liquid adds in decocting liquid, in 40-42 ℃ of aerobic fermentation 44-60 hour, 37-40 ℃ of oven dry, obtains Chinese medicine microecological preparation.
Preferably, the cultural method of described raising live pig immunity, carries out free choice feeding, drinking-water by pig farm usual manner, and 0.08%(w/w is added in immunity and sterilization feeding and management simultaneously on basal diet) described Chinese medicine microecological preparation fed.
Further preferably, described Chinese medicine microecological preparation is prepared as follows and is obtained:
(1) take 15 parts of clean and dry dried orange peels, 15 parts of the root of herbaceous peonys, 11 parts of cordate houttuynias, 8 parts of rhizoma atractylodis, 8 parts of the Radixs Astragali, be ground into particle, add for the first time 8 times of water gagings that mix medicinal material gross weight, decoct 2 hours, add for the second time 6 times of water gagings that mix medicinal material gross weight, decoct collecting decoction 1 hour, be down to room temperature, standby;
(2) press the CFU ratio of 3:1, get synanthrin lactobacillus (
sporolactobacillus inulinus) CGMCC No.2185, Rhizopus oryzae (
rhizopus oryzae) CCTCC No.M209191 bacterium liquid adds in decocting liquid, in 40-42 ℃ of aerobic fermentation 52 hours, 37-40 ℃ of oven dry, obtained Chinese medicine microecological preparation.
It is a kind of for improving the Chinese medicine microecological preparation of live pig immunity that the present invention also provides, it be prepare as follows and:
(1) take clean and dry dried orange peel 10-18 part, root of herbaceous peony 10-18 part, cordate houttuynia 8-14 part, rhizoma atractylodis 6-10 part, Radix Astragali 6-10 part, be ground into particle, add for the first time the 8-12 times of water gaging that mixes medicinal material gross weight, decoct 2 hours, add for the second time the 6-8 times of water gaging that mixes medicinal material gross weight, decoct collecting decoction 1 hour, be down to room temperature, standby;
(2) press the CFU ratio of 1-5:1, get synanthrin lactobacillus (
sporolactobacillus inulinus) CGMCC No.2185, Rhizopus oryzae (
rhizopus oryzae) CCTCC No.M209191 bacterium liquid adds in decocting liquid, in 40-42 ℃ of aerobic fermentation 44-60 hour, 37-40 ℃ of oven dry, obtains Chinese medicine microecological preparation.
Preferably, described Chinese medicine microecological preparation is prepared as follows and is obtained:
(1) take 15 parts of clean and dry dried orange peels, 15 parts of the root of herbaceous peonys, 11 parts of cordate houttuynias, 8 parts of rhizoma atractylodis, 8 parts of the Radixs Astragali, be ground into particle, add for the first time 8 times of water gagings that mix medicinal material gross weight, decoct 2 hours, add for the second time 6 times of water gagings that mix medicinal material gross weight, decoct collecting decoction 1 hour, be down to room temperature, standby;
(2) press the CFU ratio of 3:1, get synanthrin lactobacillus (
sporolactobacillus inulinus) CGMCC No.2185, Rhizopus oryzae (
rhizopus oryzae) CCTCC No.M209191 bacterium liquid adds in decocting liquid, in 40-42 ℃ of aerobic fermentation 52 hours, 37-40 ℃ of oven dry, obtained Chinese medicine microecological preparation.
Compared with prior art, the pig-breeding method tool the present invention relates to has the following advantages and is significant progressive:
(1) the alternative antibiotic of Chinese medicine microecological preparation, avoids medicament residue, solves the problem of drug resistance.(2) Performance of Finishing Pigs is had to comparatively significant facilitation.(3) improve the concentration of complement in Immunoglobulin in Serum content and serum, improved the immunologic function of growing and fattening pigs.
The specific embodiment
Be below concrete preparation example of the present invention, technical scheme of the present invention is done to further description, but protection scope of the present invention is not limited to these embodiment.Every do not deviate from the change of the present invention design or be equal to substitute include within protection scope of the present invention.
Embodiment 1
(1) take clean and dry dried orange peel 15kg, the root of herbaceous peony 15 kg, cordate houttuynia 11 kg, rhizoma atractylodis 8 kg, the Radix Astragali 8 kg, be ground into particle, add for the first time 8 times of water gagings that mix medicinal material gross weight, decoct 2 hours, add for the second time 6 times of water gagings that mix medicinal material gross weight, decoct collecting decoction 1 hour, be down to room temperature, standby;
(2) press the CFU ratio of 3:1, get synanthrin lactobacillus (
sporolactobacillus inulinus) CGMCC No.2185, Rhizopus oryzae (
rhizopus oryzae) CCTCC No.M209191 bacterium liquid adds in decocting liquid, in 40-42 ℃ of aerobic fermentation 52 hours, 37-40 ℃ of oven dry, obtained Chinese medicine microecological preparation.
By pig farm usual manner, carry out free choice feeding, drinking-water, 0.08%(w/w is added in immunity and sterilization feeding and management on basal diet simultaneously) above-mentioned Chinese medicine microecological preparation fed.Powder dry mixing material is fed, and the crib of take is principle without surplus material.Regularly clean colony house 2 times every day, keeps clean in circle.Colony house is taked gravity-flow ventilation, and irregularly sterilizes.
Embodiment 2
(1) take clean and dry dried orange peel 15kg, the root of herbaceous peony 15 kg, cordate houttuynia 11 kg, rhizoma atractylodis 8 kg, the Radix Astragali 8 kg, be ground into particle, add for the first time 8 times of water gagings that mix medicinal material gross weight, decoct 2 hours, add for the second time 6 times of water gagings that mix medicinal material gross weight, decoct collecting decoction 1 hour, be down to room temperature, standby;
(2) press the CFU ratio of 5:1, get synanthrin lactobacillus (
sporolactobacillus inulinus) CGMCC No.2185, Rhizopus oryzae (
rhizopus oryzae) CCTCC No.M209191 bacterium liquid adds in decocting liquid, in 40-42 ℃ of aerobic fermentation 52 hours, 37-40 ℃ of oven dry, obtained Chinese medicine microecological preparation.
By pig farm usual manner, carry out free choice feeding, drinking-water, 0.08%(w/w is added in immunity and sterilization feeding and management on basal diet simultaneously) above-mentioned Chinese medicine microecological preparation fed.Powder dry mixing material is fed, and the crib of take is principle without surplus material.Regularly clean colony house 2 times every day, keeps clean in circle.Colony house is taked gravity-flow ventilation, and irregularly sterilizes.
Embodiment 3
(1) take clean and dry dried orange peel 18kg, the root of herbaceous peony 10 kg, cordate houttuynia 10 kg, rhizoma atractylodis 10 kg, the Radix Astragali 10 kg, be ground into particle, add for the first time 8 times of water gagings that mix medicinal material gross weight, decoct 2 hours, add for the second time 6 times of water gagings that mix medicinal material gross weight, decoct collecting decoction 1 hour, be down to room temperature, standby;
(2) press the CFU ratio of 3:1, get synanthrin lactobacillus (
sporolactobacillus inulinus) CGMCC No.2185, Rhizopus oryzae (
rhizopus oryzae) CCTCC No.M209191 bacterium liquid adds in decocting liquid, in 40-42 ℃ of aerobic fermentation 52 hours, 37-40 ℃ of oven dry, obtained Chinese medicine microecological preparation.
By pig farm usual manner, carry out free choice feeding, drinking-water, 0.08%(w/w is added in immunity and sterilization feeding and management on basal diet simultaneously) above-mentioned Chinese medicine microecological preparation fed.Powder dry mixing material is fed, and the crib of take is principle without surplus material.Regularly clean colony house 2 times every day, keeps clean in circle.Colony house is taked gravity-flow ventilation, and irregularly sterilizes.
The impact of embodiment 4 Chinese medicine microecological preparations on growing and fattening pigs immune indexes
Select 80 of healthy commodity growing and fattening pigs (average 92 ages in days), be divided at random 2 processing, each processes 4 repetitions, and each repeats 10, and between each group, body weight difference is not remarkable.The 1st group is control group, and the basal diet of feeding processes 2 for test group, adds the Chinese medicine microecological preparation of 0.08% embodiment 1 preparation, respectively at on-test with while finishing, weighs.Experimental design is as following table.
Table 1 test grouping and Feed formulation
| Process | Diet forms |
| Control group | Basis diet |
| Test group | Basis diet+0.08% Chinese medicine microecological preparation |
The diet of test group is prepared as follows: the Chinese medicine microecological preparation 0.8g of 1kg basis diet and the embodiment of the present invention 1 preparation fully mixes, and obtains.
With reference to the nutritional need of NRC (1998) pig, the basic diet of growing and fattening pigs has the one-tenth of following percentage by weight to be grouped into: corn 65.5%, dehulled soybean meal 20%, wheat bran 10%, soya-bean oil 0.5%, premix 4%.Wherein premix is the growing and fattening pigs vitamin premix FV518 that Beijing Fuwish Biotechnology Co., Ltd produces.
In 28 days formal test cycles, blood sampling when feeding experiment finishes, adopts full automatic biochemical apparatus to measure every blood parameters, and Immunoglobulin in Serum IgA, IgG, IgM and complement C3, C4 content adopt immune turbidimetry to measure.
Each organizes complement C3 in Immunoglobulin in Serum content and serum, C4 content in Table 2.Result of the test shows, adds the concentration that Chinese medicine microecological preparation has improved complement in its Immunoglobulin in Serum content and serum in daily ration.Immunoglobulin (Ig) is also referred to as antibody, refer to a class globulin with antigentic specificity, or there is the globulin of similar chemical constitution, and the main biological function of immunoglobulin (Ig) comprises conjugated antigen and activating complement etc., it is extensively present in animal blood, tissue fluid.IgA, lgM, lgG are three major types immunoglobulin (Ig)s main in mammalian blood serum.Complement refers to an albuminoid with similar enzymatic activity, its major function be participation protective immune response to maintain body homeostasis, can help phagocyte and antibody eliminating pathogen.And C3 is the key components of complement performance functional effect, be the core component of complement system, have that conditioning promotes to engulf, immune adherence, and immunoregulatory function; C4 Main Function is the deposition that participates in regulate complement activation, neutralizes a toxin and prevent immune complex.
Table 2 Chinese medicine microecological preparation is on the impact of growing and fattening pigs immune indexes (g/L)
| Index | Control group | Test I group |
| IgA | 0.17±0.08 | 0.62±0.11 ** |
| IgG | 3.20±0.34 | 4.39±0.28 ** |
| IgM | 0.27±0.06 | 0.53±0.09 ** |
| Complement C3 | 0.09±0.02 | 0.11±0.02 |
| Complement C4 | 0.04±0.01 | 0.08±0.02 * |
With control group comparison,
* p< 0.05,
* p< 0.01.
In addition, the present invention has also verified and in daily ration, has added the impact of Chinese medicine microecological preparation on Performance of Finishing Pigs.Result shows, compares control group, adds after Chinese medicine microecological preparation in daily ration, has improved daily gain (improving 7.81%) and the daily ingestion amount (improving 6.15%) of growing and fattening pigs, has reduced material anharmonic ratio simultaneously, has effectively improved the production performance of growing and fattening pigs.
Claims (3)
1. a cultural method that improves live pig immunity, is characterized in that: the method is included in adds 0.05%-0.1%(w/w on basal diet) Chinese medicine microecological preparation fed; Described Chinese medicine microecological preparation is prepared as follows and is obtained:
(1) take clean and dry dried orange peel 10-18 part, root of herbaceous peony 10-18 part, cordate houttuynia 8-14 part, rhizoma atractylodis 6-10 part, Radix Astragali 6-10 part, be ground into particle, add for the first time the 8-12 times of water gaging that mixes medicinal material gross weight, decoct 2 hours, add for the second time the 6-8 times of water gaging that mixes medicinal material gross weight, decoct collecting decoction 1 hour, be down to room temperature, standby;
(2) press the CFU ratio of 1-5:1, get synanthrin lactobacillus (
sporolactobacillus inulinus) CGMCC No.2185, Rhizopus oryzae (
rhizopus oryzae) CCTCC No.M209191 bacterium liquid adds in decocting liquid, in 40-42 ℃ of aerobic fermentation 44-60 hour, 37-40 ℃ of oven dry, obtains Chinese medicine microecological preparation.
2. improve according to claim 1 the cultural method of live pig immunity, it is characterized in that: by pig farm usual manner, carry out free choice feeding, drinking-water, 0.08%(w/w is added in immunity and sterilization feeding and management on basal diet simultaneously) described Chinese medicine microecological preparation fed.
3. improve according to claim 2 the cultural method of live pig immunity, it is characterized in that: described Chinese medicine microecological preparation is prepared as follows and obtained:
(1) take 15 parts of clean and dry dried orange peels, 15 parts of the root of herbaceous peonys, 11 parts of cordate houttuynias, 8 parts of rhizoma atractylodis, 8 parts of the Radixs Astragali, be ground into particle, add for the first time 8 times of water gagings that mix medicinal material gross weight, decoct 2 hours, add for the second time 6 times of water gagings that mix medicinal material gross weight, decoct collecting decoction 1 hour, be down to room temperature, standby;
(2) press the CFU ratio of 3:1, get synanthrin lactobacillus (
sporolactobacillus inulinus) CGMCC No.2185, Rhizopus oryzae (
rhizopus oryzae) CCTCC No.M209191 bacterium liquid adds in decocting liquid, in 40-42 ℃ of aerobic fermentation 52 hours, 37-40 ℃ of oven dry, obtained Chinese medicine microecological preparation.
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| CN103704482B (en) * | 2013-12-31 | 2016-01-13 | 南方医科大学 | A kind of poultry Chinese herbal feed additive and its preparation method and application |
| CN105146216A (en) * | 2015-10-15 | 2015-12-16 | 格特生物制药（天津）有限公司 | Fermented traditional Chinese medicine preparation capable of reducing diarrhea ratio of piglets |
| CN105707488A (en) * | 2016-02-29 | 2016-06-29 | 河南牧业经济学院 | Traditional Chinese medicine microecologics for enhancing piglet immunity and preparing method of traditional Chinese medicine microecologics |
| CN105670967A (en) * | 2016-03-04 | 2016-06-15 | 朝阳市德凤生态养殖有限责任公司 | Microecological ferment for fattening pig feeds and preparation method thereof, and active fermentation feed |
| CN107223780A (en) * | 2017-06-12 | 2017-10-03 | 金乡县春柳生猪定点屠宰厂 | A kind of pig Chinese herbal feed additive of alternative antibiotic |
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| CN100581378C (en) * | 2006-08-01 | 2010-01-20 | 济南亿民动物药业有限公司 | Method for preparing fermented Chinese herbal medicine preparations for feed |
| CN102232998B (en) * | 2011-04-15 | 2013-01-30 | 北京康华远景科技有限公司 | Micro-ecological traditional Chinese medicine preparation for enhancing immunity of livestock and poultry, and preparation method thereof |
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Denomination of invention: Farming method capable of improving immunity of live pigs Effective date of registration: 20190125 Granted publication date: 20140122 Pledgee: China Merchants Bank Limited by Share Ltd Hefei branch Pledgor: Anhui Changfeng Animal Husbandry Technology Co., Ltd. Registration number: 2019340000062 |
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Date of cancellation: 20190308 Granted publication date: 20140122 Pledgee: China Merchants Bank Limited by Share Ltd Hefei branch Pledgor: Anhui Changfeng Animal Husbandry Technology Co., Ltd. Registration number: 2019340000062 |
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Denomination of invention: Farming method capable of improving immunity of live pigs Effective date of registration: 20190409 Granted publication date: 20140122 Pledgee: China Merchants Bank Limited by Share Ltd Hefei branch Pledgor: Anhui Changfeng Animal Husbandry Technology Co., Ltd. Registration number: 2019340000190 |