CN103053403A - Functional eucommia ulmoides bean sprouts and production method thereof - Google Patents
Functional eucommia ulmoides bean sprouts and production method thereof Download PDFInfo
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Abstract
The invention belongs to the technical field of bean sprouts and in particular discloses functional eucommia ulmoides bean sprouts and a production method thereof. The method comprises the following steps of: 1, extracting the following components in percentage by weight: 30-90 percent of eucommia ulmoides leaves, 0-60 percent of eucommia ulmoides bark and 0-12 percent of eucommia ulmoides male flower by adding water or ethanol, filtering, adding vitamin C which accounts for 0.01-0.1 mass percent into the filtrate, regulating the pH value of the solution to be between 3.0 and 6.0 by using alimentary acetic acid, and obtaining an eucommia ulmoides nutrient solution; and 2, soaking the bean seeds by using the eucommia ulmoides nutrient solution, spraying the beans by using the eucommia ulmoides nutrient solution or/and water, and cultivating to obtain the bean sprouts according to the conventional bean sprout cultivation method. According to the method, the eucommia ulmoides resources such as eucommia ulmoides leaves, eucommia ulmoides bark (branches) and eucommia ulmoides male flower are fully utilized, a novel path for integrated eucommia ulmoides utilization is developed, the prepared eucommia ulmoides bean sprouts contain chlorogenic acid and other active ingredients which contribute to body health, a novel green functional food is provided, and the health-care effect of the traditional bean sprouts is improved.
Description
Technical field
The invention belongs to the bean sprouts production technical field, particularly a kind of functional form bark of eucommia bean sprouts and production method thereof.
Background technology
China is production bean sprouts country the earliest, and seedling obbbin bed, the layer stereos of adopting are put cultivation mode and carried out soilless culture more now.The bean sprouts is as a kind of Vegetables, with its delicious flavour, nutritious, have the good characteristic such as health-care efficacy and very popular, its production method is easy, production technology is easy to grasp, production efficiency is high.Bean sprouts's growth cycle is short, needn't apply fertilizer in the process of growth, and suitable temperature, the sufficient moisture of assurance can rely on the nutrient growth of seed self storage, endanger without agricultural chemicals.
Especially like with the darkest consumer of being subjected to of mung bean sprouts among the bean sprouts that Japan, a large amount of mung beans of the annual import of the U.S. are used for the production of mung bean sprouts.Mung bean amino acid content in germination process increases substantially (content of glutamic acid is the highest); Lipoid material is resolved into glycerine and fatty acid by enzyme, generates at last the carbohydrate that can be absorbed by the body; Carbohydrate then is hydrolyzed to the monose that is easy to absorption of human body, has improved absorbability, and total sugar content generally reduces; Vitamin kind (Vc, VA, nicotinic acid, carotin etc.) content all increases; Mineral matter (potassium, magnesium, calcium, iron, phosphorus) content increases substantially after soaking germination.
Mung bean sprouts has very high medical value.The traditional Chinese medical science is thought, mung bean sprouts flavor cool in nature is sweet, the clear hot summer weather of energy, qualcomm meridian, all poison of solution, kidney tonifying, diuresis, detumescence, nourishing and strengthening vital, transfer the five internal organs, U.S. skin, dampness removing heat, be applicable to damp and hot stasis, eat the patients such as few body is tired, pyreticosis polydipsia, constipation, difficult urination, red eye, swell pain, the living sore of mouth and nose, can also reducing blood lipid and softening blood vessel.
Eucommiaceae plant bark of eucommia Eucommia ulmoides Oliv. is that the Jue that the fourth season, the glacier carried over loses plant, is China second class protection plant.Bark of Eucommia Ulmoides is China's tradition rare traditional Chinese medicine, the energy filling liver kidney, and strengthening the bones and muscles, it is anti-old to make light of one's life by commiting suicide, and is used for kidney deficiency and liver, ache of the spinal column, muscles and bones is unable, has a dizzy spell etc.Folium cortex eucommiae is for " Chinese pharmacopoeia one one of version in 2010 is recorded kind, and eucommia bark, leaf and male flower contain similar active component and effect.Modern pharmacological research shows: the health-care effect of the bark of eucommia have strengthen immunity, delay senility, the effect such as prevention of osteoporosis.Folium cortex eucommiae and male flower come off naturally with seasonal variation, seldom are exploited, and have caused a large amount of wastes of eucommia resource.
Ascorbic pharmacological action: 1. promote the biosynthesis of collagen, be beneficial to the faster healing of tissue injury mouth, strengthen skin elasticity; 2. have anti-oxidantly, the effect of detoxifcation reduces side effect that cigarette, wine, medicine produce and environmental pollution to the infringement of health; 3. promote absorption and the utilization of iron, calcium, folic acid; 4. improve fat and the particularly metabolism of cholesterol of lipoid, the prevention cardiovascular disease; 5. promote the growth of tooth and bone, prevent that gum is hemorrhage; 6. strengthen body to external world stress ability and the immunity of environment.
At present, the bean sprouts quality is single, and function bean sprouts with health role is less, along with the enhancing of people's health care consciousness, and the particularly quickening of China's aging population process, market in urgent need has the bean sprouts of health care.
Summary of the invention
For overcoming deficiency of the prior art, the object of the present invention is to provide a kind of functional form bark of eucommia bean sprouts and production method thereof.
For achieving the above object, the technical scheme taked of the present invention is as follows:
The production method of a kind of functional form bark of eucommia bean sprouts may further comprise the steps:
The first step: in weight percentage, add water or alcohol extract by folium cortex eucommiae 30 ~ 90%, Bark of Eucommia Ulmoides 0 ~ 60%, eucommia Bark male flower 0 ~ 12%, filter, add the vitamin C that accounts for its percentage by weight 0.01 ~ 0.1% in the filtrate, modulate this liquid pH value between 3.0 ~ 6.0 with alimentary acetic acid, get nutritive eucommia bark liquid;
Second step: with nutritive eucommia bark liquid soaking beans kind,, cultivate and get final product according to conventional bean sprout cultivation method or/and water sprays with nutritive eucommia bark liquid.
Preferably, the first step: in weight percentage, add water or alcohol extract by folium cortex eucommiae 50 ~ 70%, Bark of Eucommia Ulmoides 20 ~ 40%, eucommia Bark male flower 4 ~ 10%, filter, add the vitamin C that accounts for its percentage by weight 0.05% in the filtrate, modulate this liquid pH value between 3.0 ~ 6.0 with alimentary acetic acid, get nutritive eucommia bark liquid.
Preferably, described folium cortex eucommiae obtains by the following method: gather green folium cortex eucommiae annual 6～September, dries or oven dry below 60 ℃; Described Bark of Eucommia Ulmoides obtains by the following method: the branch that annual 4 ~ September, the bark of eucommia was pruned is peelled off Bark of Eucommia Ulmoides, dries; Described eucommia Bark male flower obtains by the following method: gather that the eucommia Bark male flower bud completes, drying annual March.
Preferably, extract 1 ~ 4 time, and 5 ~ 50 volume/weight of at every turn pressing folium cortex eucommiae, Bark of Eucommia Ulmoides, eucommia Bark male flower total amount extraordinarily water or ethanol, wherein volume/weight is in ml/g.
Further, extracting mode extracts for soaking extraction, heating and refluxing extraction or sudden strain of a muscle formula, soaks extraction conditions: 4～80 ℃ of temperature, time 0.5～12h; Refluxing extraction condition: time 0.2～3h; Sudden strain of a muscle formula extraction conditions: 15～60 ℃ of temperature, time 0.1～30min.
Preferably, described beans kind is mung bean, soya bean, French beans, pea, black soya bean, peanut, green soya bean, Chinese toon seed or Semen Raphani.
A kind of functional form bark of eucommia bean sprouts of aforementioned production method preparation.
Advantage of the present invention is:
1, by enforcement of the present invention, take full advantage of the eucommia resources such as folium cortex eucommiae, Bark of Eucommia Ulmoides (branch), eucommia Bark male flower, open up the new way of bark of eucommia comprehensive utilization, provide a kind of novel green functional food to people, promoted the health-care effect of traditional bean sprouts.
2, the present invention is directed to the single problem of bean sprouts production kind, utilize Bark of Eucommia Ulmoides, leaf, male flower to make nutritive eucommia bark liquid for raw material, cultivate a kind of functional form " bark of eucommia bean sprouts ", by to bark of eucommia bean sprouts active ingredient contrast test, find that " bark of eucommia bean sprouts " contain the active component that chlorogenic acid etc. is conducive to health, for the exploitation of functional bean sprouts provides effective approach and Research Thinking.
Description of drawings
Fig. 1: chlorogenic acid reference substance HPLC figure;
Fig. 2: embodiment 1 functional form bark of eucommia bean sprouts HPLC figure;
Fig. 3: embodiment 2 functional form bark of eucommia bean sprouts HPLC figure;
Fig. 4: embodiment 3 functional form bark of eucommia bean sprouts HPLC figure.
Embodiment
Below in conjunction with specific embodiment technical scheme of the present invention is done further in detail introduction, but protection scope of the present invention is not limited to this:
The first step: gather green folium cortex eucommiae annual July, dries; The branch that annual May, the bark of eucommia was pruned is peelled off Bark of Eucommia Ulmoides, dries.Gather that the eucommia Bark male flower bud completes, drying annual March.
Second step: following raw material is ground into fine powder by folium cortex eucommiae 60g, Bark of Eucommia Ulmoides 32g, eucommia Bark male flower 8g, extract 1 time with the 2500ml water soaking, extraction time is 8 hours, extracting temperature is 30 ℃, filter, account for the vitamin C of its percentage by weight 0.01% filtrate adding, modulates this liquid pH value 4.0 with alimentary acetic acid, namely gets nutritive eucommia bark liquid.
The 3rd step: replace the water soaking mung bean with nutritive eucommia bark liquid, with nutritive eucommia bark liquid spray, cultivate and get final product according to conventional bean sprout cultivation method.As: get the 200g mung bean and put into nutritive eucommia bark liquid 600ml, 25 ℃ of constant temperature soaked 12 hours, after the abundant imbibition of seed, were transferred in the case of bean sprouts, cultivated the bean sprouts, and temperature keeps 25 ℃.Every day according to the above ratio with method preparation nutritive eucommia bark liquid, keeps fresh, every day is with per 6 hours sprays of nutritive eucommia bark liquid bean sprouts 1 time, generally in the case of bean sprouts 3 ~ 4 days, during radicle plumular axis length to the 3 centimetre left and right sides, just obtains functional form bark of eucommia mung bean sprouts after washing with clear water.
The first step: gather green folium cortex eucommiae, 55 ℃ of oven dry annual August; Gather that the eucommia Bark male flower bud completes, drying annual March.
Second step: following raw material is pressed folium cortex eucommiae 90g, eucommia Bark male flower 10g, adopt flash extracter (ZL 200520031604.9) to extract 4 times, each 1500ml water of using, extracted 5 minutes, extracting temperature is 28 ℃, merges extract, filter, account for the vitamin C of its percentage by weight 0.1% filtrate adding, modulates this liquid pH value 5.5 with edible malic acid, namely gets nutritive eucommia bark liquid.
The 3rd step: replace the water soaking mung bean with nutritive eucommia bark liquid, with nutritive eucommia bark liquid spray, cultivate and get final product according to conventional bean sprout cultivation method.
The first step: gather green folium cortex eucommiae annual June, dries; The branch that annual April, the bark of eucommia was pruned is peelled off Bark of Eucommia Ulmoides, dries.
Second step: following raw material is pressed folium cortex eucommiae 53g, Bark of Eucommia Ulmoides 47g, extract 3 times with the 40v% alcohol reflux, each 1200ml 40v% ethanol, extraction time is 2 hours, merges extract, filter, decompression filtrate recycling ethanol filters to without the alcohol flavor, accounts for the vitamin C of its percentage by weight 0.05% filtrate adding, modulate this liquid pH value 4.5 with edible citric acid, namely get nutritive eucommia bark liquid.
The 3rd step: soak mung bean with nutritive eucommia bark liquid, the water spray is cultivated and be get final product according to conventional bean sprout cultivation method.
Functional form bark of eucommia mung bean sprouts Content of Chlorogenic Acid assay method
1, instrument and reagent
The Shimadzu(Shimadzu) LC ﹣ 2010AHT high performance liquid chromatograph, the Shimadzu(Shimadzu) SPD ﹣ 10A UV-detector; Zhejiang University's intelligence reaches the N2000 chromatographic work station; Diamonsil
TMC
18Chromatographic column (5 μ m, 150mm * 4.6mm) (Di Ma company).AG135 type electronic balance (METILER TOLEDO); Ultrasonic cleaner (KQ5200DA type, Kunshan Ultrasonic Instruments Co., Ltd.); The chlorogenic acid reference substance (National Institute for Food and Drugs Control provides, for assay, and lot number: 110753-200413); Methyl alcohol is chromatographically pure (Merk company); Water is ultra-pure water, and other reagent are pure for analyzing.
Chromatographic condition and system suitability octadecylsilane chemically bonded silica are filler; Mobile phase: methanol-water-glacial acetic acid (volume ratio, 15:85:1.5); Detect wavelength: 237nm; Flow velocity: 1mL/min; Column temperature: 25 ℃.Number of theoretical plate calculates by the chlorogenic acid peak and is not less than 2000.
It is an amount of that the chlorogenic acid reference substance is got in the preparation of reference substance solution, accurately weighed, puts in the brown measuring bottle, adds 50v% methyl alcohol and make the solution that every 1ml contains 50 μ g, and get final product.
The preparation of need testing solution is got this product fresh sample and is ground, and accurately weighed about 5 g put in the tool plug conical flask, add 50 v % methyl alcohol 25ml, weighed weight added hot reflux 30 minutes, let cool, filter, filtrate decompression is concentrated into and is less than 10ml, let cool, weighed weight adds 50v% methyl alcohol and supplies the weight that subtracts mistake again, shake up, filter, get subsequent filtrate, and get final product.
Determination method is accurate reference substance solution and each 10 μ l of need testing solution of drawing respectively, and the injection liquid chromatography is measured, and be get final product.
The content of reference substance solution and embodiment 1-3 functional form bark of eucommia mung bean sprouts Content of Chlorogenic Acid sees Table 1, Fig. 1-4.
Claims (7)
1. the production method of a functional form bark of eucommia bean sprouts is characterized in that may further comprise the steps:
The first step: in weight percentage, add water or alcohol extract by folium cortex eucommiae 30 ~ 90%, Bark of Eucommia Ulmoides 0 ~ 60%, eucommia Bark male flower 0 ~ 12%, filter, add the vitamin C that accounts for its percentage by weight 0.01 ~ 0.1% in the filtrate, modulate this liquid pH value between 3.0 ~ 6.0 with food acids, get nutritive eucommia bark liquid;
Second step: with nutritive eucommia bark liquid soaking beans kind,, cultivate and get final product according to conventional bean sprout cultivation method or/and water sprays with nutritive eucommia bark liquid.
2. production method as claimed in claim 1, it is characterized in that the first step: in weight percentage, add water or alcohol extract by folium cortex eucommiae 50 ~ 70%, Bark of Eucommia Ulmoides 20 ~ 40%, eucommia Bark male flower 4 ~ 10%, filter, add the vitamin C that accounts for its percentage by weight 0.05% in the filtrate, modulate this liquid pH value between 3.0 ~ 6.0 with food acids, get nutritive eucommia bark liquid.
3. production method as claimed in claim 1, it is characterized in that: described folium cortex eucommiae obtains by the following method: gather green folium cortex eucommiae annual 6～September, dries or oven dry below 60 ℃; Described Bark of Eucommia Ulmoides obtains by the following method: the branch that annual 4 ~ September, the bark of eucommia was pruned is peelled off Bark of Eucommia Ulmoides, dries; Described eucommia Bark male flower obtains by the following method: gather that the eucommia Bark male flower bud completes, drying annual March.
4. production method as claimed in claim 1 is characterized in that: extracts 1 ~ 4 time, and 5 ~ 50 volume/weight of at every turn pressing folium cortex eucommiae, Bark of Eucommia Ulmoides, eucommia Bark male flower total amount extraordinarily water or ethanol, wherein volume/weight is in ml/g.
5. production method as claimed in claim 4 is characterized in that: extracting mode extracts for soaking extraction, heating and refluxing extraction or sudden strain of a muscle formula, soaks extraction conditions: 4～80 ℃ of temperature, time 0.5～12h; Refluxing extraction condition: time 0.2～3h; Sudden strain of a muscle formula extraction conditions: 15～60 ℃ of temperature, time 0.1～30min.
6. production method as claimed in claim 1, it is characterized in that: described beans kind is mung bean, soya bean, French beans, pea, black soya bean, peanut, green soya bean, Chinese toon seed or Semen Raphani.
7. the functional form bark of eucommia bean sprouts of the described production method preparation of any one of a utilization such as claim 1 ~ 6.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104739928A (en) * | 2013-12-25 | 2015-07-01 | 李春启 | Composition having blood sugar-lowering and lipid-lowering effects |
| CN105104135A (en) * | 2015-08-21 | 2015-12-02 | 贵州茅台流域东升农场有限公司 | Culture method for accelerating bean sprouting |
| CN112673747A (en) * | 2020-12-19 | 2021-04-20 | 胡宪明 | Cultivation method of bean seedling vegetables with specific efficacy |
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| KR20050058840A (en) * | 2003-12-12 | 2005-06-17 | 한국과학기술연구원 | Method for growing nutraceutical bean sprout using circular cultivation system, bean sprout produced thereby, method for preparing dried product therefrom and its use |
| KR100826233B1 (en) * | 2007-01-05 | 2008-04-30 | 코지 하세가와 | The manufacturing method of the bean sprout |
| KR20090002554A (en) * | 2007-07-02 | 2009-01-09 | 김두호 | The method for bean sprouts from kalopanax sap |
| CN102726277A (en) * | 2012-07-14 | 2012-10-17 | 黄仁洙 | Preparation method of chitin bean sprout |
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- 2013-01-31 CN CN201310037956.4A patent/CN103053403B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20050058840A (en) * | 2003-12-12 | 2005-06-17 | 한국과학기술연구원 | Method for growing nutraceutical bean sprout using circular cultivation system, bean sprout produced thereby, method for preparing dried product therefrom and its use |
| KR100826233B1 (en) * | 2007-01-05 | 2008-04-30 | 코지 하세가와 | The manufacturing method of the bean sprout |
| KR20090002554A (en) * | 2007-07-02 | 2009-01-09 | 김두호 | The method for bean sprouts from kalopanax sap |
| CN102726277A (en) * | 2012-07-14 | 2012-10-17 | 黄仁洙 | Preparation method of chitin bean sprout |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104739928A (en) * | 2013-12-25 | 2015-07-01 | 李春启 | Composition having blood sugar-lowering and lipid-lowering effects |
| CN105104135A (en) * | 2015-08-21 | 2015-12-02 | 贵州茅台流域东升农场有限公司 | Culture method for accelerating bean sprouting |
| CN112673747A (en) * | 2020-12-19 | 2021-04-20 | 胡宪明 | Cultivation method of bean seedling vegetables with specific efficacy |
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