CN103043804A - Method for treating soybean processing wastewater by utilizing photosynthetic bacteria and recycling wastewater - Google Patents
Method for treating soybean processing wastewater by utilizing photosynthetic bacteria and recycling wastewater Download PDFInfo
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- CN103043804A CN103043804A CN2012105742422A CN201210574242A CN103043804A CN 103043804 A CN103043804 A CN 103043804A CN 2012105742422 A CN2012105742422 A CN 2012105742422A CN 201210574242 A CN201210574242 A CN 201210574242A CN 103043804 A CN103043804 A CN 103043804A
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Abstract
The invention discloses a method for treating soybean processing wastewater by utilizing photosynthetic bacteria and recycling the wastewater, and belongs to the technical field of wastewater treatment. The method comprises the steps as follows: adding small molecular carbon source substances with the final concentration of 400-1200 mg/L and small molecular nitrogen source substances with the final concentration of 200-2000 mg/L into the soybean processing wastewater at first; adjusting the pH value of the wastewater to be 7.0-9.0; adding rhodobacter sphaeroides with the final concentration of 400-1500 mg/L during logarithmic growth phase into the wastewater; treating for 96-120 h at the temperature of 25-30 DEG C; and micro-aerating in the wastewater and controlling the dissolved oxygen concentration in the soybean processing wastewater to be 0.5-1.0 mg/L during the treatment process. According to the method, the energy consumption is reduced, the process of treating sewage with the photosynthetic bacteria is simplified, a large amount of bacterial resources capable of being directly and comprehensively utilized are produced, and the problem of secondary pollution is avoided, so that the sewage recycling rate is increased.
Description
Technical field
The invention belongs to technical field of waste water processing, particularly a kind of method of utilizing photosynthetic bacterium processed soybeans processing waste water and realizing waste water reclaiming.
Background technology
Soybean processing waste water is the larger class waste water of quantity discharged in the food-processing industry.Soybean processing waste water is nontoxic, is rich in the macromole nutritive substances such as a large amount of polysaccharide, protein and Mierocrystalline cellulose.Known soybean processing waste water treatment process has coagulating sedimentation, catalyzed oxidation, materialization-acidication-contact oxidation method, upflow anaerobic sludge blanket process etc., but these methods in use can produce a large amount of excess sludges, it is high that it processes disposal costs, the resource utilization degree is low, easily causes secondary pollution.
Use photosynthetic bacterium processed soybeans processing waste water can realize simultaneously the removal of Pollutants in Wastewater and the recovery of resource.The life condition of photosynthetic bacterium is wide in range, and it has two cover energy metabolism systems, can survive under illumination anaerobism and dark aerobic condition, thereby the assimilation low molecule organic matter is realized removal of pollutants; Simultaneously, photosynthetic bacterial thallus is nontoxic, can directly be fully utilized in industries such as feed processing without processing, and has the great potential of resource utilization.Traditional photosynthetic bacterium sewage treatment process need to add pretreatment section before the photosynthetic bacterium processing section, purpose is that the macromolecular substance that first a part of photosynthetic bacterium in the waste water can not directly be utilized is converted into that carbochain is short, the easier small-molecule substance that is utilized by photosynthetic bacterium, such as volatile acid etc.General pretreatment process is anaerobic digestion at present.Although the anaerobic digestion link has certain contribution to removal of pollutants in whole technical process, whole technique also there is certain disadvantageous effect.At first, the employed device operational administrative of anaerobic digestion is complicated, and energy consumption is higher, disagrees with the low-carbon (LC) sewage treatment process purport that existing country advocates; Secondly, a large amount of anerobes that anaerobic process is introduced are caused infection to photosynthetic bacterium easily, thereby can weaken the dominant microflora status of photosynthetic bacterium, reduce the ability of photosynthetic bacterium degradation of contaminant and the resource utilization degree of technique; At last, anaerobic process still can produce excess sludge, can not avoid secondary pollution.
Summary of the invention
For solve pretreatment process brings in traditional photosynthetic bacterium waste water treatment process complex process, the problem such as the sewage recycling degree is low, secondary pollution is serious, the present invention proposes a kind of method of utilizing photosynthetic bacterium processed soybeans processing waste water and realizing waste water reclaiming.
Describedly utilize photosynthetic bacterium processed soybeans processing waste water and realize that the method for waste water reclaiming comprises the steps:
At first adding the small molecules carbon source material in the soybean processing waste water is that 400 ~ 1200 mg/L and small molecules nitrogen source are 200 ~ 2000 mg/L to final concentration to final concentration;
The pH value of re-adjustment soybean processing waste water is 7.0 ~ 9.0;
Then adding the red bacterium of class ball that is in logarithmic phase in the soybean processing waste water is 400 ~ 1500 mg/L to final concentration;
Treatment condition are natural lighting, and treatment temp is 25 ~ 30 ℃, and the treatment time is 96 ~ 120 hours; In soybean processing waste water, carry out little aeration by aeration pump in the treating processes, use traffic meter control aeration intensity, and use the dissolved oxygen instrument Real-Time Monitoring, dissolved oxygen concentration is at 0.5 ~ 1.0 mg/L in the control soybean processing waste water.
Wherein, described small molecules carbon source material is one or more in glucose, fructose, Seignette salt, succsinic acid, sodium succinate, oxysuccinic acid, tartrate, glycerine, yeast extract paste extractive substance, ethanol, sodium acetate, pyruvic acid, lactic acid, the mannitol;
Described small molecules nitrogen source is one or more in ammonium sulfate, ammonium chloride, L-glutamic acid, peptone, aspartic acid and the urea;
Described soybean processing waste water is a kind of waste water that is rich in the macromole Carbon and nitrogen sources materials such as macromolecular polysaccharide, protein and fat, mainly exists with macromolecular substance states such as particle nutrition, colloids; The chemical oxygen demand (COD) of soybean processing waste water (COD) is 8000 ~ 30000 mg/L, and total nitrogen (TN) is 70 ~ 80 mg/L, and total phosphorus (TP) is 40 ~ 50mg/L, and dissolved organic carbon (DOC) is 200 ~ 250mg/L, and the pH value is 4.0 ~ 6.0;
The Rhodobacter Sphaeroides that the red bacterium of class ball that the present invention uses belongs in the photosynthetic bacterium belongs to, (address: Yard 1, BeiChen xi Road, Chaoyang District, Beijing City institute of microbiology of the Chinese Academy of Sciences), deposit number is CGMCC No.1.2174 available from Chinese common micro-organisms culture presevation administrative center.
Beneficial effect of the present invention is:
Provided by the inventionly utilize photosynthetic bacterium processed soybeans processing waste water and realize that the method for waste water reclaiming can greatly improve sewage treating efficiency, realize sewage recycling.COD and protein removal rate in the final sewage can reach respectively more than 90% and 85%, and the thalline rate of increase is 200% ~ 600%, than COD clearance in traditional photosynthetic bacterium sewage water treatment method and thalline output high 20 ~ 40% and 200 ~ 700%.
The present invention is except adding small molecules carbon source material sodium succinate in soybean processing waste water, utilize the ability of macromole nitrogen source in the sewage in order to increase photosynthetic bacterium, also in soybean processing waste water, added small molecules nitrogen source ammonium sulfate, for the growth of photosynthetic bacterium provides initial carbon source, nitrogenous source and the energy.Suitable illumination, Oxygen Condition have strengthened the cytoactive of photosynthetic bacterium, stimulate the key enzyme of degraded macromolecular substance to produce, thereby the macromolecular substance such as polysaccharide, protein and fat in the soybean processing waste water are further utilized by photosynthetic bacterium, realize degraded and the conversion of macromolecular substance in the soybean processing waste water.This mode more is conducive to the photosynthetic bacterium degradation of contaminant and improves photosynthetic bacterial thallus output.Therefore, in the processing disposal that solves excess sludge, secondary pollution problems, produced useful thalline resource a large amount of, that can be used for plant husbandry, animal husbandry and fishery.
In addition, the present invention adopts natural lighting to add the means of limited aeration, and the dissolved oxygen content in the waste water is controlled at 0.5 ~ 1.0 mg/L, has saved energy consumption than traditional photosynthetic bacterium sewage treatment process.The method is easy and simple to handle, can omit the pretreatment section of traditional photosynthetic bacterium sewage treatment process, thus simplification of flowsheet; Meanwhile, the present invention can also improve waste water treatment efficiency and waste water the resource utilization degree, avoid the environmental problems such as secondary pollution.
Description of drawings
Fig. 1 be embodiment 1, embodiment 2, embodiment 3 with Comparative Examples 1 and Comparative Examples 2 in COD degradation rate trend map over time in the soybean processing waste water;
Wherein X-coordinate is the time, and unit is hour; Ordinate zou is the COD clearance, and unit is per-cent.
Fig. 2 be embodiment 1, embodiment 2, embodiment 3 with Comparative Examples 1 and Comparative Examples 2 in protein degradation rate trend map over time in the soybean processing waste water;
Wherein X-coordinate is the time, and unit is hour; Ordinate zou is the protein removal rate, and unit is per-cent.
Fig. 3 be embodiment 1, embodiment 2, embodiment 3 with Comparative Examples 1 and Comparative Examples 2 in photosynthetic bacterium output trend map over time in the soybean processing waste water;
Wherein X-coordinate is the time, and unit is hour; Ordinate zou is thalline output, and unit is mg/L.
Embodiment
The present invention will be further described in detail below in conjunction with accompanying drawing and specific embodiment:
Embodiment 1:
At first adding sodium succinate in the soybean processing waste water is that 725 mg/L and ammonium sulfate are 400mg/L to final concentration to final concentration;
The pH value of re-adjustment soybean processing waste water is 7.0;
Then adding the red bacterium of class ball in the soybean processing waste water is 400 mg/L to final concentration;
Treatment condition are natural lighting, and treatment temp is 25 ~ 30 ℃, and the treatment time is 96 hours; In soybean processing waste water, carry out little aeration by aeration pump in the treating processes, use traffic meter control aeration intensity, and use the dissolved oxygen instrument Real-Time Monitoring, dissolved oxygen concentration is at 0.5 ~ 1.0 mg/L in the control soybean processing waste water.The numerical value of COD, protein and thalline output in sewage of 24 hours mensuration.
Embodiment 2:
At first adding sodium succinate in the soybean processing waste water is that 450 mg/L and ammonium sulfate are 300mg/L to final concentration to final concentration;
The pH value of re-adjustment soybean processing waste water is 7.0;
Then adding the red bacterium of class ball in the soybean processing waste water is 819 mg/L to final concentration;
Treatment condition are natural lighting, and treatment temp is 25 ~ 30 ℃, and the treatment time is 120 hours; In soybean processing waste water, carry out little aeration by aeration pump in the treating processes, use traffic meter control aeration intensity, and use the dissolved oxygen instrument Real-Time Monitoring, dissolved oxygen concentration is at 0.5 ~ 1.0 mg/L in the control soybean processing waste water.The numerical value of COD, protein and thalline output in sewage of 24 hours mensuration.
Embodiment 3:
At first adding sodium succinate in the soybean processing waste water is that 1000 mg/L and ammonium sulfate are 1500mg/L to final concentration to final concentration;
The pH value of re-adjustment soybean processing waste water is 7.0;
Then adding the red bacterium of class ball in the soybean processing waste water is 1214 mg/L to final concentration;
Treatment condition are natural lighting, and treatment temp is 25 ~ 30 ℃, and the treatment time is 120 hours; In soybean processing waste water, carry out little aeration by aeration pump in the treating processes, use traffic meter control aeration intensity, and use the dissolved oxygen instrument Real-Time Monitoring, dissolved oxygen concentration is at 0.5 ~ 1.0 mg/L in the control soybean processing waste water.The numerical value of COD, protein and thalline output in sewage of 24 hours mensuration.
Comparative Examples 1:
Compare with embodiment 1, do not add small molecules carbon source material sodium succinate and small molecules nitrogen source ammonium sulfate, all the other operation stepss and condition are with embodiment 1.
Comparative Examples 2:
Compare with embodiment 1, the Dissolved Oxygen concentration Control in the soybean processing waste water is at 2.0 ~ 2.5 mg/L, and all the other operation stepss and condition are with embodiment 1.
Result and analysis:
As shown in Figure 1: after 96 ~ 120 hours processing, the COD clearance of soybean processing waste water reaches respectively 95.2%, 96.0%, 97.5%, 61.2% and 68.3% in embodiment 1, embodiment 2, embodiment 3, Comparative Examples 1 and the Comparative Examples 2.Illustrate and added after small molecules carbon source material sodium succinate and the small molecules nitrogen source ammonium sulfate that the red bacterium of class ball has improved 34.0 ~ 36.3% to the COD degradation rate of soybean processing waste water; In addition, when dissolved oxygen content was controlled at 0.5 ~ 1.0 mg/L in the soybean processing waste water, the red bacterium of class ball was higher by 26.9% ~ 29.2% when 2.0 ~ 2.5 mg/L than dissolved oxygen content to the degradation rate of COD; Small molecules carbon source material sodium succinate and small molecules nitrogen source ammonium sulfate that this explanation is added have strengthened the activity of photosynthetic bacterium, have effectively improved sewage treating efficiency; Simultaneously, the oxygen of trace more is conducive to the degraded of pollutent.
As shown in Figure 2: after 96 ~ 120 hours processing, the clearance of the protein of soybean processing waste water reaches respectively 89.7%, 92.6%, 93.7%, 58.1% and 64.6% in embodiment 1, embodiment 2, embodiment 3, Comparative Examples 1 and the Comparative Examples 2.Illustrate and added after small molecules carbon source material sodium succinate and the small molecules nitrogen source ammonium sulfate that the red bacterium of class ball has improved 31.6% ~ 35.6% to the degradation rate of protein in the soybean processing waste water.In addition, when dissolved oxygen content was controlled at 0.5 ~ 1.0 mg/L in the soybean processing waste water, the red bacterium of class ball was higher by 25.1% ~ 29.1% when 2.0 ~ 2.5 mg/L than dissolved oxygen content to the degradation rate of protein.
As shown in Figure 3: after 96 ~ 120 hours processing, thalline output in embodiment 1, embodiment 2, embodiment 3, Comparative Examples 1 and the Comparative Examples 2 reaches respectively 2512.5,4168.9,4729.6,827.0 and 800.4 mg/L, productive rate is respectively 528.1%, 409.0%, 289.6%, 106.8% and 100.1%.Illustrate and added after small molecules carbon source material sodium succinate and the small molecules nitrogen source ammonium sulfate, and under the condition that trace oxygen exists, thalline output is the highest.
Claims (6)
1. the method utilizing photosynthetic bacterium processed soybeans processing waste water and realize waste water reclaiming is characterized in that described method comprises the steps:
At first adding the small molecules carbon source material in the soybean processing waste water is that 400 ~ 1200 mg/L and small molecules nitrogen source are 200 ~ 2000 mg/L to final concentration to final concentration;
The pH value of re-adjustment soybean processing waste water is 7.0 ~ 9.0;
Then adding the red bacterium of class ball in the soybean processing waste water is 400 ~ 1500 mg/L to final concentration;
Treatment condition are natural lighting, and treatment temp is 25 ~ 30 ℃, and the treatment time is 96 ~ 120 hours; In soybean processing waste water, carry out little aeration by aeration pump in the treating processes, use traffic meter control aeration intensity, and use the dissolved oxygen instrument Real-Time Monitoring, dissolved oxygen concentration is at 0.5 ~ 1.0 mg/L in the control soybean processing waste water.
2. method according to claim 1 is characterized in that, the chemical oxygen demand (COD) of described soybean processing waste water is 8000 ~ 30000 mg/L, and total nitrogen is 70 ~ 80 mg/L, and total phosphorus is 40 ~ 50mg/L, and dissolved organic carbon is 200 ~ 250mg/L, and the pH value is 4.0 ~ 6.0.
3. method according to claim 1, it is characterized in that described small molecules carbon source material is one or more in glucose, fructose, Seignette salt, succsinic acid, sodium succinate, oxysuccinic acid, tartrate, glycerine, yeast extract paste extractive substance, ethanol, sodium acetate, pyruvic acid, lactic acid, the mannitol.
4. method according to claim 1 is characterized in that, described small molecules nitrogen source is one or more in ammonium sulfate, ammonium chloride, L-glutamic acid, peptone, aspartic acid and the urea.
5. method according to claim 1 is characterized in that, the red bacterium of described class ball is in logarithmic phase.
6. method according to claim 1 is characterized in that, the Rhodobacter Sphaeroides that the red bacterium of described class ball belongs in the photosynthetic bacterium belongs to, and available from Chinese common micro-organisms culture presevation administrative center, deposit number is CGMCC No.1.2174.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN105461082A (en) * | 2016-01-06 | 2016-04-06 | 中国人民大学 | Method for processing and recycling soybean processing wastewater through photosynthetic bacteria |
| CN105502684A (en) * | 2016-01-05 | 2016-04-20 | 中国人民大学 | Method for treating high-ammonia nitrogen wastewater through multiple microorganisms |
| CN110127854A (en) * | 2018-02-02 | 2019-08-16 | 中国农业大学 | Utilize the method for graphene quantum dot resource utilization waste water |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN105502684A (en) * | 2016-01-05 | 2016-04-20 | 中国人民大学 | Method for treating high-ammonia nitrogen wastewater through multiple microorganisms |
| CN105502684B (en) * | 2016-01-05 | 2018-08-14 | 中国人民大学 | A kind of method of composite flora processing high ammonia-nitrogen wastewater |
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| CN110127854A (en) * | 2018-02-02 | 2019-08-16 | 中国农业大学 | Utilize the method for graphene quantum dot resource utilization waste water |
| CN110127854B (en) * | 2018-02-02 | 2021-05-28 | 中国农业大学 | Method for recycling wastewater by utilizing graphene quantum dots |
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Application publication date: 20130417 |