CN103041882A - Microfluidic chip for detecting polycyclic aromatic hydrocarbon in atmosphere and preparation method of microfluidic chip - Google Patents

Microfluidic chip for detecting polycyclic aromatic hydrocarbon in atmosphere and preparation method of microfluidic chip Download PDF

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CN103041882A
CN103041882A CN2012105896079A CN201210589607A CN103041882A CN 103041882 A CN103041882 A CN 103041882A CN 2012105896079 A CN2012105896079 A CN 2012105896079A CN 201210589607 A CN201210589607 A CN 201210589607A CN 103041882 A CN103041882 A CN 103041882A
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chip
aromatic hydrocarbon
polycyclic aromatic
sample
micro
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沙俊
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SUZHOU WENHAO CHIP TECHNOLOGY Co Ltd
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SUZHOU WENHAO CHIP TECHNOLOGY Co Ltd
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    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/01Arrangements or apparatus for facilitating the optical investigation
    • G01N21/03Cuvette constructions
    • G01N21/07Centrifugal type cuvettes
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502707Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the manufacture of the container or its components
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/17Systems in which incident light is modified in accordance with the properties of the material investigated
    • G01N21/25Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
    • G01N21/31Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
    • G01N21/35Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry using infrared light
    • G01N21/3577Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry using infrared light for analysing liquids, e.g. polluted water
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00Additional constructional details
    • B01L2300/08Geometry, shape and general structure
    • B01L2300/0803Disc shape
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00Additional constructional details
    • B01L2300/08Geometry, shape and general structure
    • B01L2300/0861Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/087Multiple sequential chambers
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00Additional constructional details
    • B01L2300/08Geometry, shape and general structure
    • B01L2300/0887Laminated structure
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00Moving or stopping fluids
    • B01L2400/04Moving fluids with specific forces or mechanical means
    • B01L2400/0403Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0409Moving fluids with specific forces or mechanical means specific forces centrifugal forces

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  • Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
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Abstract

The invention relates to a microfluidic chip for detecting polycyclic aromatic hydrocarbon in atmosphere and a preparation method of the microfluidic chip. The microfluidic chip is a disk-shaped centrifugal microfluidic chip with microstructures and microchannels and is formed by sealing a plurality of chips and double-layer sticky films, wherein the chips are engraved with micron-sized microstructures and micro-channels; centrifugal force is adopted as driving force for sample microfluid to fulfil the mixing, extracting and separating processes of a sample to be detected and an extracting agent; and an infrared spectrometer is adopted as a detection device to quantificationally or qualitatively detect the contents of oil and grease in the sample. Through the microfluidic chip and the method, a plurality of groups of samples can be treated and detected simultaneously; the consumption of reagents and samples is low; integration, microminiaturization and automation in detection of polycyclic aromatic hydrocarbon are realized; the characteristics of economy, quickness, portability and high efficiency are achieved; and a brand-new analysis technology platform is provided for convenient, quick and accurate detection of polycyclic aromatic hydrocarbon.

Description

A kind of centrifugal type microfludic chip that detects polycyclic aromatic hydrocarbon in the atmosphere and preparation method thereof
Technical field
The present invention relates to a kind of micro-fluidic chip that detects polycyclic aromatic hydrocarbon in the atmosphere and preparation method thereof; this chip is the disc-shaped centrifugal type microfludic chip; prepare micro-structural and microchannel on the surface by micro-processing technology; under the centrifugal force that the centrifuge rotation produces drives; realize testing sample and extractant mixing, extract, separate and filter process; with infrared spectrometer quantitatively or qualitative detection go out the content of polycyclic aromatic hydrocarbon in the chip loading product, be mainly used in the association areas such as environmental protection and water quality detection.
Background technology
Polycyclic aromatic hydrocarbon (polycyclic aromatic hydrocarbon) is the hydrocarbon that contains two above phenyl ring in the molecule, comprises that naphthalene, anthracene, phenanthrene, pyrene etc. more than 150 plant compound, and it is present in crude oil, creosote; tar, dyestuff, plastics; rubber, lubricating oil, antirust oil; remover, gasoline anticoagulant, electric capacity electrolyte; mineral oil in the petroleum chemicals such as pitch, also is present in agricultural chemicals; charcoal, bactericide is in the daily chemical products such as mosquito-repellent incense.People are in the polycyclic aromatic hydrocarbons contaminated environment for a long time, can cause acute or chronic injury.And polycyclic aromatic hydrocarbon is also confirmed by test already to the carcinogenesis of animal.Therefore, the fast detecting of polycyclic aromatic hydrocarbon is of great significance at tool aspect environmental science, the food security science.
The standard method of polycyclic aromatic hydrocarbon routine forms by designing sampling, transportation, storage, at last extraction processing and GC/MS quantitative analysis, and these class methods exist amount of samples large, and sample pre-treatments is complicated, and the shortcoming that cost is higher needs to spend plenty of time and cost.And sample separation process and detect and separately independently to carry out, easily cause sample loss and pollution.By above reason, set up a kind of small-sized integrating device, quick, portable, accurate polycyclic aromatic hydrocarbon checkout gear is very important.
In recent years, micro-fluidic chip is as a kind of novel analysis platform, has microminiaturization, automation, the advantage such as integrated, convenient and quick, obtains a wide range of applications in the environment measuring association area.Yet, adopt the disc-shaped chip, utilize micro-processing technology to be used for analyzing in its surface preparation micro-structural and microchannel, rely on centrifugal force to drive the sample microfluid, finish simultaneously the centrifugal type microfludic chip analytical technology of a plurality of sample detection, in the fast detecting related application field of polycyclic aromatic hydrocarbon substantial breakthrough is arranged not yet at present.
Summary of the invention
The purpose of this invention is to provide a kind of centrifugal type microfludic chip that detects polycyclic aromatic hydrocarbon in the atmosphere and preparation method thereof, it is characterized in that this chip is the centrifugal type microfludic chip of disc-shaped band micro-structural and microchannel, the micro-structural and the chip of microchannel, the double-deck adhesive membrane involution that are carved with micron level by multilayer form, with the driving force of centrifugal force as the sample microfluid, finish sample and extractant mixing, extract, separate and the filter process process, take infrared spectrometer as checkout gear, quantitatively or qualitatively detect on the chip content of polycyclic aromatic hydrocarbon in the sample.Micro-structural and microchannel prepare by micro-processing technology, comprise abstraction pool, filtering ponds, detection cell, little valve, microchannel, microflute and micropore.The rotation platform of centrifuge aims at the disc-shaped sample design, and the center is with sucker, draw-in groove or screw-nut fixed wafer shape centrifugal type microfludic chip.
For achieving the above object, the present invention adopts following operating procedure:
1. Computer-aided design Software for Design and draw micro-structural and the microchannel figure of each layer chip in the centrifugal type microfludic chip;
2. prepare required micro-structural and microchannel by micro-processing technology in the processing of each layer micro-fluidic chip substrate surface, comprise abstraction pool, filtering ponds, detection cell, little valve, microchannel, microflute and micropore;
3. testing sample is put into abstraction pool;
4. utilize double-deck adhesive membrane, with each leafing core type micro-fluidic chip align, bonding, pressurizing and sealing, form centrifugal type microfludic chip;
5. behind the chip involution, in abstraction pool, add extractant;
6. startup centrifuge, testing sample and extractant mix under centrifugal action, extraction and fractionation;
7. change centrifugal speed, extract shift by little valve and enter filtering ponds, carry out Separation of Solid and Liquid in this pond;
8. again change centrifugal speed, clarification extract after filtering shifts by little valve and enters detection cell;
Sample treatment good after, detect extraction solution at infrared spectrometer, obtain the content of polycyclic aromatic hydrocarbon.
Among the present invention, the chip substrates that detects the centrifugal type microfludic chip of polycyclic aromatic hydrocarbon in the atmosphere can be PMMA, PC, PVC, COC, also commercially available all kinds of CD CDs.
Among the present invention, detecting the micro-structural of the centrifugal type microfludic chip of polycyclic aromatic hydrocarbon in the atmosphere and microchannel can be by numerical control mill quarter, laser ablation, LIGA technology, method of molding, pressure sintering, chemical attack preparation, also available soft lithographic technique preparation.
Among the present invention, detect between each layer chip of the centrifugal type microfludic chip of polycyclic aromatic hydrocarbon in the atmosphere with double-deck adhesive membrane involution, double-deck adhesive membrane can be that power causes double-deck adhesive membrane, also common double faced adhesive tape film.
Among the present invention, the width and the degree of depth that are connected the microchannel between the abstraction pool that detects the centrifugal type microfludic chip of polycyclic aromatic hydrocarbon in the atmosphere and the filtering ponds are 380 μ m, insert the quartz capillary of external diameter 375 μ m as little valve, the internal diameter of quartz capillary can be 100 μ m, 150 μ m.
Among the present invention, the width and the degree of depth that are connected the microchannel between the filtering ponds that detect the centrifugal type microfludic chip of polycyclic aromatic hydrocarbon in the atmosphere and the detection cell are 380 μ m, insert the quartz capillary of external diameter 375 μ m as little valve, the internal diameter of quartz capillary can be 25,50,75,100 μ m.
Among the present invention, the centrifugal type microfludic chip that detects polycyclic aromatic hydrocarbon in the atmosphere need not any external pump and valve, utilize centrifugal force to drive the sample microfluid, when extractant and sample mix extraction, centrifuge speed is 150~300 rev/mins, the centrifugal rotational speed that extraction solution enters filtering ponds is 500~700 rev/mins, and the centrifugal rotational speed that enters detection cell is 800~1000 rev/mins.
Among the present invention, the reagent that detects the centrifugal type microfludic chip extracting polyaromatic hydrocarbon of polycyclic aromatic hydrocarbon in the atmosphere is tetrachloro-ethylene and carbon tetrachloride, and toxicity is less than carrene and dimethyl sulfoxide (DMSO), has reduced the impact on environment.
The disc-shaped centrifugal type microfludic chip of polycyclic aromatic hydrocarbon in the detection atmosphere that the present invention proposes, can realize the Parallel testing of a plurality of samples, reduced the consumption of reagent and sample, simplified the pre-treatment of testing sample, shortened detection time, have portable, economic, characteristics fast and efficiently, the association area of polycyclic aromatic hydrocarbon has a good application prospect in the fast detecting atmosphere.
Description of drawings
Fig. 1. detect five layers of chip of the centrifugal type microfludic chip of polycyclic aromatic hydrocarbon in the atmosphere and the structural representation of one group of sample analysis detecting unit.
A. the 1st layer (bottom chip, involution is used), the 2nd layer of (double-deck adhesive membrane of B., bonding usefulness), the 3rd layer of C. (middle master chip has micro-structural and microchannel), D. the 4th layer (double-deck adhesive membrane, bonding usefulness), the 5th layer of (upper strata chip of E., involution usefulness) structural representation of one group of sample analysis detecting unit of F.: 1) abstraction pool, 2) filtering ponds, 3) detection cell, 4) the rectangular capillary passage, 5) quartz capillary passage, 6) gas microchannel and hole.
Specific embodiments
Embodiment 1
Micro-structural and the microchannel figure of five layers of chip of Computer-aided design Software for Design and drafting centrifugal type microfludic chip.In each strata methyl methacrylate (PMMA) chip preparation micro-structural and microchannel, clean each layer chip with running water, distilled water by numerical control CNC micro-machining system respectively, and with spots such as the residual fingerprint of ethanol chip surface, oil stains.30mg atmospheric sample to be measured is positioned in the abstraction pool, with five layers of chip carefully align, bonding, pressurizing and sealing, make the centrifugal type microfludic chip that detects polycyclic aromatic hydrocarbon in the atmosphere.Centrifugal type microfludic chip is fixed on the centrifuge rotation platform by sucker, adds 100 μ L C from the sample hand-hole 2Cl 4, mix with 150 rev/mins rotating speeds, behind the extraction 10min, improve rotating speed to 500 rev/min, extraction solution in the sample cell is all moved to filtering ponds; Improve subsequently centrifugal rotational speed to 800 rev/min, will clarify extract and all be transferred to detection cell, carry out absorbance detection at the 334nm place, obtain at last the content of polycyclic aromatic hydrocarbon in the sample.
Embodiment 2
Micro-structural and the microchannel figure of five layers of chip of Computer-aided design Software for Design and drafting centrifugal type microfludic chip.In each strata carbonic ester (PC) chip preparation micro-structural and microchannel, clean each layer chip with running water, distilled water respectively by numerical control CNC micro-machining system at last, and with spots such as the residual fingerprint of ethanol chip surface, oil stains.30mg atmospheric sample to be measured is positioned in the abstraction pool, with five layers of chip carefully align, bonding, pressurizing and sealing, make the centrifugal type microfludic chip that detects polycyclic aromatic hydrocarbon in the atmosphere.Centrifugal type microfludic chip is fixed on the centrifuge rotation platform by sucker, adds 100 μ L C from the sample hand-hole 2Cl 4, mix with 300 rev/mins rotating speeds, behind the extraction 5min, improve centrifuge speed to 700 rev/min, extraction solution in the sample cell is all moved to filtering ponds; Improve subsequently centrifuge speed to 1000 rev/min, will clarify extract and all be transferred to detection cell, carry out absorbance detection at the 334nm place, obtain at last the content of polycyclic aromatic hydrocarbon in the sample.

Claims (9)

1. centrifugal type microfludic chip that detects polycyclic aromatic hydrocarbon in the atmosphere and preparation method thereof, it is characterized in that, this chip is the centrifugal type microfludic chip of disc-shaped band micro-structural and microchannel, the micro-structural and the chip of microchannel, the double-deck adhesive membrane involution that are carved with micron level by multilayer form, with the driving force of centrifugal force as the sample microfluid, finish testing sample and extractant mixing, extract, separate and filter process, take infrared spectrometer as checkout gear, quantitatively or qualitatively detect on the chip content of polycyclic aromatic hydrocarbon fat in the sample.Micro-structural and microchannel prepare by micro-processing technology, comprise abstraction pool, filtering ponds, detection cell, little valve, microchannel, microflute and micropore.The rotation platform of centrifuge aims at the disc-shaped sample design, and the center is with sucker, draw-in groove or screw-nut fixed wafer shape centrifugal type microfludic chip.
2. press centrifugal type microfludic chip of polycyclic aromatic hydrocarbon in the detection atmosphere claimed in claim 1 and preparation method thereof, it is characterized in that its making step is as follows:
1) micro-structural and the microchannel figure of each layer chip in Computer-aided design Software for Design and the drafting centrifugal type microfludic chip;
2) prepare required micro-structural and microchannel by micro-processing technology in the processing of each layer micro-fluidic chip substrate surface, comprise abstraction pool, filtering ponds, detection cell, little valve, microchannel, microflute and micropore;
3) testing sample is put into abstraction pool;
4) utilize double-deck adhesive membrane, with each leafing core type micro-fluidic chip align, bonding, pressurizing and sealing, form centrifugal type microfludic chip;
5) behind the chip involution, in abstraction pool, add extractant;
6) start centrifuge, testing sample and extractant mix under centrifugal action, extraction and fractionation;
7) change centrifugal speed, extract shifts by little valve and enters filtering ponds, carries out Separation of Solid and Liquid in this pond;
8) again change centrifugal speed, clarification extract after filtering shifts by little valve and enters detection cell;
9) sample treatment good after, detect extraction solution at infrared spectrometer, obtain the content of polycyclic aromatic hydrocarbon.
3. press centrifugal type microfludic chip of polycyclic aromatic hydrocarbon in claim 1 or the 2 described detection atmosphere and preparation method thereof, it is characterized in that, the Core Feature device of this polycyclic aromatic hydrocarbon detection chip is the centrifugal type microfludic chip that disc-shaped has micro-structural and microchannel, the size of micro-structural and microchannel is micron level, and the micro-processing method by numerical control mill quarter, laser ablation, LIGA technology, method of molding, pressure sintering, chemical attack, soft lithographic technique is in the preparation of chip substrates surface.The disc-shaped centrifugal type microfludic chip can be produced in batches, repeatedly utilization, flexible design and assembling.
4. press centrifugal type microfludic chip of polycyclic aromatic hydrocarbon in claim 1 or the 2 described detection atmosphere and preparation method thereof, it is characterized in that, this polycyclic aromatic hydrocarbon detection chip need not the pump of any external connection, the sample microfluid be under centrifugal force drives, mix with extraction agent, extraction and fractionation.
5. press centrifugal type microfludic chip of polycyclic aromatic hydrocarbon in claim 1 or the 2 described detection atmosphere and preparation method thereof, it is characterized in that, this polycyclic aromatic hydrocarbon detection chip need not the sample introduction pre-treatment, can on chip, directly finish sample pretreatment and detection, and detect simultaneously the content of polycyclic aromatic hydrocarbon in the sample.
6. press centrifugal type microfludic chip of polycyclic aromatic hydrocarbon in claim 1 or the 2 described detection atmosphere and preparation method thereof, it is characterized in that, this polycyclic aromatic hydrocarbon detection chip can be made many group micro-structurals and microchannel at chip piece, consist of many group sample analysis detecting units, improve the Parallel testing ability of unit interval.
7. press centrifugal type microfludic chip of polycyclic aromatic hydrocarbon in claim 1 or the 2 described detection atmosphere and preparation method thereof, it is characterized in that, little valve of Quality control microfluidic flow is embedded in the microchannel by quartz capillary and consists of, and regulates and control the flow regime of microfluid by the internal diameter of control quartz capillary.
8. press centrifugal type microfludic chip of polycyclic aromatic hydrocarbon in claim 1 or the 2 described detection atmosphere and preparation method thereof, it is characterized in that, this polycyclic aromatic hydrocarbon detection chip is by changing step by step the rotating speed of centrifuge, make extract be transferred to filtering ponds from abstraction pool by rectangular capillary, be transferred to detection cell from filtering ponds by quartz capillary subsequently, the internal diameter of quartz capillary is less, and the sample microfluid is just larger by the required centrifugal force of this quartz capillary, and required centrifugal rotational speed is just higher.
9. press centrifugal type microfludic chip of polycyclic aromatic hydrocarbon in claim 1 or the 2 described detection atmosphere and preparation method thereof, it is characterized in that, this polycyclic aromatic hydrocarbon detection chip design is ingenious, equipment is simple, be easy to carry, Direct Sampling, need not sample treatment, sample and reagent dosage are little, the Parallel testing ability is high, easily be connected with detector, determination and testing process are finished at same chip, sample need not to shift, sample cross pollution probability is little, satisfy well the needs of micro-total analysis system development, convenient, fast, the association area that detects exactly polycyclic aromatic hydrocarbon is with a wide range of applications.
CN2012105896079A 2012-12-31 2012-12-31 Microfluidic chip for detecting polycyclic aromatic hydrocarbon in atmosphere and preparation method of microfluidic chip Pending CN103041882A (en)

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Cited By (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103055983A (en) * 2012-12-31 2013-04-24 苏州汶颢芯片科技有限公司 Centrifugal micro-fluidic chip for detecting polycyclic aromatic hydrocarbon in soil and preparation method thereof
CN103055979A (en) * 2012-12-31 2013-04-24 苏州汶颢芯片科技有限公司 Centrifugal micro-fluidic chip for detecting polycyclic aromatic hydrocarbon in water and preparation method thereof
CN105331528A (en) * 2014-08-14 2016-02-17 天津市农业质量标准与检测技术研究所 Microfluidics detecting chip based on nucleic acid amplification technology and preparation method thereof
CN108043478A (en) * 2017-12-04 2018-05-18 国家纳米科学中心 A kind of micro-fluidic chip, manual centrifugal device and nucleic acid detection method
CN109655585A (en) * 2018-12-29 2019-04-19 佛山市云米电器科技有限公司 A kind of kitchen ventilator that can identify kitchen air quality
CN109681937A (en) * 2018-12-29 2019-04-26 佛山市云米电器科技有限公司 A kind of kitchen ventilator that can identify harmful substance in oil smoke
CN109882898A (en) * 2018-12-29 2019-06-14 佛山市云米电器科技有限公司 It is a kind of with identification oil smoke in pernicious gas and the kitchen ventilator of particulate matter function
CN110237775A (en) * 2019-06-18 2019-09-17 金华职业技术学院 A kind of micro liquid hybrid reaction method
EP3632563A4 (en) * 2017-05-22 2020-05-27 Tianjin Mnchip Technologies Co. Ltd. Chip for sample detection and packaging method thereof
WO2020248203A1 (en) * 2019-06-13 2020-12-17 王锦弘 Centrifugal reaction microtube, centrifugal reaction device and centrifugal test method therefor

Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2423266A (en) * 2004-11-25 2006-08-23 Ind Tech Res Inst A structure and method for detecting an analyte in a microfluidic chip
CN101561391A (en) * 2009-06-04 2009-10-21 中国航空工业集团公司西安飞机设计研究所 Gas concentration measuring device and measuring method thereof
CN101884941A (en) * 2009-12-30 2010-11-17 复旦大学 Biological detection microfluidic chip based on immune reaction and preparation method thereof
EP2490020A1 (en) * 2011-02-18 2012-08-22 Koninklijke Philips Electronics N.V. Measurement chip, microfluidic device and method of measurement chip manufacture
CN102784673A (en) * 2012-08-13 2012-11-21 苏州汶颢芯片科技有限公司 Centrifugal micro-fluidic chip for detecting oil and grease and preparation method of centrifugal micro-fluidic chip
CN103055979A (en) * 2012-12-31 2013-04-24 苏州汶颢芯片科技有限公司 Centrifugal micro-fluidic chip for detecting polycyclic aromatic hydrocarbon in water and preparation method thereof
CN103055983A (en) * 2012-12-31 2013-04-24 苏州汶颢芯片科技有限公司 Centrifugal micro-fluidic chip for detecting polycyclic aromatic hydrocarbon in soil and preparation method thereof

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2423266A (en) * 2004-11-25 2006-08-23 Ind Tech Res Inst A structure and method for detecting an analyte in a microfluidic chip
CN101561391A (en) * 2009-06-04 2009-10-21 中国航空工业集团公司西安飞机设计研究所 Gas concentration measuring device and measuring method thereof
CN101884941A (en) * 2009-12-30 2010-11-17 复旦大学 Biological detection microfluidic chip based on immune reaction and preparation method thereof
EP2490020A1 (en) * 2011-02-18 2012-08-22 Koninklijke Philips Electronics N.V. Measurement chip, microfluidic device and method of measurement chip manufacture
CN102784673A (en) * 2012-08-13 2012-11-21 苏州汶颢芯片科技有限公司 Centrifugal micro-fluidic chip for detecting oil and grease and preparation method of centrifugal micro-fluidic chip
CN103055979A (en) * 2012-12-31 2013-04-24 苏州汶颢芯片科技有限公司 Centrifugal micro-fluidic chip for detecting polycyclic aromatic hydrocarbon in water and preparation method thereof
CN103055983A (en) * 2012-12-31 2013-04-24 苏州汶颢芯片科技有限公司 Centrifugal micro-fluidic chip for detecting polycyclic aromatic hydrocarbon in soil and preparation method thereof

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
席永清,庄惠生: "离心式微流控液相萃取法测定水和土壤中油和油脂", 《农业环境科学学报》, vol. 29, no. 10, 31 October 2010 (2010-10-31), pages 2045 - 2048 *

Cited By (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103055983A (en) * 2012-12-31 2013-04-24 苏州汶颢芯片科技有限公司 Centrifugal micro-fluidic chip for detecting polycyclic aromatic hydrocarbon in soil and preparation method thereof
CN103055979A (en) * 2012-12-31 2013-04-24 苏州汶颢芯片科技有限公司 Centrifugal micro-fluidic chip for detecting polycyclic aromatic hydrocarbon in water and preparation method thereof
CN105331528A (en) * 2014-08-14 2016-02-17 天津市农业质量标准与检测技术研究所 Microfluidics detecting chip based on nucleic acid amplification technology and preparation method thereof
CN105331528B (en) * 2014-08-14 2018-05-08 天津市农业质量标准与检测技术研究所 A kind of micro-fluidic detection chip based on nucleic acid amplification technologies and preparation method thereof
EP3632563A4 (en) * 2017-05-22 2020-05-27 Tianjin Mnchip Technologies Co. Ltd. Chip for sample detection and packaging method thereof
CN108043478A (en) * 2017-12-04 2018-05-18 国家纳米科学中心 A kind of micro-fluidic chip, manual centrifugal device and nucleic acid detection method
CN109655585A (en) * 2018-12-29 2019-04-19 佛山市云米电器科技有限公司 A kind of kitchen ventilator that can identify kitchen air quality
CN109681937A (en) * 2018-12-29 2019-04-26 佛山市云米电器科技有限公司 A kind of kitchen ventilator that can identify harmful substance in oil smoke
CN109882898A (en) * 2018-12-29 2019-06-14 佛山市云米电器科技有限公司 It is a kind of with identification oil smoke in pernicious gas and the kitchen ventilator of particulate matter function
CN109655585B (en) * 2018-12-29 2021-08-31 佛山市云米电器科技有限公司 Range hood capable of identifying kitchen air quality
WO2020248203A1 (en) * 2019-06-13 2020-12-17 王锦弘 Centrifugal reaction microtube, centrifugal reaction device and centrifugal test method therefor
CN110237775A (en) * 2019-06-18 2019-09-17 金华职业技术学院 A kind of micro liquid hybrid reaction method

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Application publication date: 20130417