CN102784673A - Centrifugal micro-fluidic chip for detecting oil and grease and preparation method of centrifugal micro-fluidic chip - Google Patents
Centrifugal micro-fluidic chip for detecting oil and grease and preparation method of centrifugal micro-fluidic chip Download PDFInfo
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- CN102784673A CN102784673A CN2012102854483A CN201210285448A CN102784673A CN 102784673 A CN102784673 A CN 102784673A CN 2012102854483 A CN2012102854483 A CN 2012102854483A CN 201210285448 A CN201210285448 A CN 201210285448A CN 102784673 A CN102784673 A CN 102784673A
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- 238000002360 preparation method Methods 0.000 title claims abstract description 22
- 238000001514 detection method Methods 0.000 claims abstract description 32
- 238000000605 extraction Methods 0.000 claims abstract description 14
- 238000000034 method Methods 0.000 claims abstract description 14
- 239000004809 Teflon Substances 0.000 claims abstract description 11
- 229920006362 Teflon® Polymers 0.000 claims abstract description 11
- 238000005516 engineering process Methods 0.000 claims abstract description 11
- 238000004458 analytical method Methods 0.000 claims abstract description 8
- 239000003153 chemical reaction reagent Substances 0.000 claims abstract description 5
- 230000003287 optical effect Effects 0.000 claims abstract description 5
- 238000007789 sealing Methods 0.000 claims abstract description 5
- 238000002156 mixing Methods 0.000 claims abstract description 4
- 239000003795 chemical substances by application Substances 0.000 claims abstract 2
- 239000000853 adhesive Substances 0.000 claims description 12
- 230000001070 adhesive effect Effects 0.000 claims description 12
- 239000012528 membrane Substances 0.000 claims description 12
- 238000012545 processing Methods 0.000 claims description 12
- 239000010453 quartz Substances 0.000 claims description 9
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N silicon dioxide Inorganic materials O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims description 9
- 238000012360 testing method Methods 0.000 claims description 9
- 238000013461 design Methods 0.000 claims description 7
- 239000000126 substance Substances 0.000 claims description 6
- 239000000284 extract Substances 0.000 claims description 4
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- 238000011282 treatment Methods 0.000 claims description 3
- 238000000608 laser ablation Methods 0.000 claims description 2
- 238000000465 moulding Methods 0.000 claims description 2
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- 230000033772 system development Effects 0.000 claims description 2
- 239000000523 sample Substances 0.000 claims 20
- 239000013068 control sample Substances 0.000 claims 1
- 238000003672 processing method Methods 0.000 claims 1
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- 238000012546 transfer Methods 0.000 claims 1
- 239000002355 dual-layer Substances 0.000 abstract 1
- 239000012530 fluid Substances 0.000 abstract 1
- 230000010354 integration Effects 0.000 abstract 1
- 239000003921 oil Substances 0.000 description 37
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 7
- IAZDPXIOMUYVGZ-UHFFFAOYSA-N Dimethylsulphoxide Chemical compound CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 description 4
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 4
- UHOVQNZJYSORNB-UHFFFAOYSA-N Benzene Chemical compound C1=CC=CC=C1 UHOVQNZJYSORNB-UHFFFAOYSA-N 0.000 description 3
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- 239000000203 mixture Substances 0.000 description 3
- 239000003208 petroleum Substances 0.000 description 3
- AFABGHUZZDYHJO-UHFFFAOYSA-N 2-Methylpentane Chemical compound CCCC(C)C AFABGHUZZDYHJO-UHFFFAOYSA-N 0.000 description 2
- 239000004215 Carbon black (E152) Substances 0.000 description 2
- 238000010521 absorption reaction Methods 0.000 description 2
- 239000002390 adhesive tape Substances 0.000 description 2
- 239000012153 distilled water Substances 0.000 description 2
- 150000002148 esters Chemical class 0.000 description 2
- DCAYPVUWAIABOU-UHFFFAOYSA-N hexadecane Chemical compound CCCCCCCCCCCCCCCC DCAYPVUWAIABOU-UHFFFAOYSA-N 0.000 description 2
- 229930195733 hydrocarbon Natural products 0.000 description 2
- 150000002430 hydrocarbons Chemical class 0.000 description 2
- 238000002329 infrared spectrum Methods 0.000 description 2
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- 238000002798 spectrophotometry method Methods 0.000 description 2
- VZGDMQKNWNREIO-UHFFFAOYSA-N tetrachloromethane Chemical compound ClC(Cl)(Cl)Cl VZGDMQKNWNREIO-UHFFFAOYSA-N 0.000 description 2
- 230000001988 toxicity Effects 0.000 description 2
- 231100000419 toxicity Toxicity 0.000 description 2
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 description 1
- 239000004713 Cyclic olefin copolymer Substances 0.000 description 1
- VVQNEPGJFQJSBK-UHFFFAOYSA-N Methyl methacrylate Chemical compound COC(=O)C(C)=C VVQNEPGJFQJSBK-UHFFFAOYSA-N 0.000 description 1
- CYTYCFOTNPOANT-UHFFFAOYSA-N Perchloroethylene Chemical group ClC(Cl)=C(Cl)Cl CYTYCFOTNPOANT-UHFFFAOYSA-N 0.000 description 1
- XUIMIQQOPSSXEZ-UHFFFAOYSA-N Silicon Chemical compound [Si] XUIMIQQOPSSXEZ-UHFFFAOYSA-N 0.000 description 1
- 239000004411 aluminium Substances 0.000 description 1
- XAGFODPZIPBFFR-UHFFFAOYSA-N aluminium Chemical compound [Al] XAGFODPZIPBFFR-UHFFFAOYSA-N 0.000 description 1
- 229910052782 aluminium Inorganic materials 0.000 description 1
- 244000145845 chattering Species 0.000 description 1
- 229910052802 copper Inorganic materials 0.000 description 1
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- 238000011161 development Methods 0.000 description 1
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- 239000011521 glass Substances 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000011017 operating method Methods 0.000 description 1
- 239000012994 photoredox catalyst Substances 0.000 description 1
- 239000004417 polycarbonate Substances 0.000 description 1
- 239000004800 polyvinyl chloride Substances 0.000 description 1
- 230000005180 public health Effects 0.000 description 1
- 229910052710 silicon Inorganic materials 0.000 description 1
- 239000010703 silicon Substances 0.000 description 1
- 229910001220 stainless steel Inorganic materials 0.000 description 1
- 239000010935 stainless steel Substances 0.000 description 1
- 238000010561 standard procedure Methods 0.000 description 1
- 229950011008 tetrachloroethylene Drugs 0.000 description 1
- 238000009423 ventilation Methods 0.000 description 1
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
- G01N21/31—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
- G01N21/35—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry using infrared light
- G01N21/3577—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry using infrared light for analysing liquids, e.g. polluted water
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/01—Arrangements or apparatus for facilitating the optical investigation
- G01N21/03—Cuvette constructions
- G01N21/07—Centrifugal type cuvettes
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- Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Spectroscopy & Molecular Physics (AREA)
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Abstract
The invention relates to a centrifugal micro-fluidic chip for detecting oil and grease and a preparation method of the centrifugal micro-fluidic chip. The centrifugal micro-fluidic chip is a disk type centrifugal micro-fluidic chip with a microstructure and a microchannel; the centrifugal micro-fluidic chip is formed by sealing multiple layers of chips on which micro structures and micro channels with micro levels are etched, a Teflon film used as an infrared optical view window and a dual-layer sticky film; the mixing, extracting and separating process of a sample to be measured and an extraction agent is finished by taking a centrifugal force as a driving force of sample micro fluid; and the contents of the oil and the grease in the sample on each chip are quantitatively or qualitatively detected by taking an infrared spectrometer as a detection device. According to the micro-fluidic chip and the method, a plurality of groups of samples can be simultaneously treated and detected; reagents and samples are fewer, and thus the integration, the micromation and the automation of the oil and the grease are realized; and the centrifugal micro-fluidic chip has the characteristics of economy, quickness, portability and high efficiency and provides a brand-new analysis technology for detecting the oil and the grease conveniently, quickly and quantitatively.
Description
Technical field
The present invention relates to a kind of micro-fluidic chip that detects oil and grease and preparation method thereof; This chip is a disk shape centrifugal type microfludic chip; In surface preparation micro-structural and microchannel, the centrifugal force that produces in centrifuge rotation drives down through micro-processing technology, the mixing of realization testing sample and extractant, extraction and separation process; With infrared spectrometer quantitatively or qualitative detection go out on the chip oil and content of oil and grease in the sample, be mainly used in association areas such as environmental protection and water quality detection.
Background technology
Serious " leakage " accident usually takes place in the commercial production, reveals in the material hydrocarbon the most commonly, like oil product; Though toxicity is little usually to reveal thing; But in a large amount of leakages of inappropriate zone generation, will cause pollution, aerobic/anaerobism disorder takes place in the biological chain that causes revealing the zone; Also can pollute, threaten and jeopardize people's health underground water.Therefore, fast detecting oil and grease have crucial meaning aspect environmental protection and the water quality detection.
GB analytical method and American Public Health Association regulation is to the analyzing detecting method of petroleum substance concentration different per sample; Infrared spectrophotometer, gravimetric method, non-dispersion infrared AAS are arranged; Wherein infrared spectrophotometer is a kind of analyzing detecting method the most commonly used, also is the standard method that the definite oily substance of State Bureau of Environmental Protection is measured.Petroleum substance is because CH
2, CH
3, CH hydrocarbon key chattering, in specific wave number characteristic absorption is arranged, available in view of the above infrared spectrophotometric determination sample carries out detection by quantitative in the absorption at specific wave number place; Highly sensitive with the infrared spectrophotometric determination petroleum substance, it is accurate to measure the result, the method good linearity; But this method exists amount of samples big, and sample pre-treatments is complicated, the shortcoming that cost is higher; And the sample separation process is separately independently carried out with detecting; Be prone to cause sample loss and pollution, be not suitable for field quick detection, also do not meet the direction of micro-total analysis system development.
Therefore, press for the novel oil and the grease detection method of a kind of quick, efficient, convenient, low-cost, automation of development, collection sample pretreatment and detection one.In recent years, micro-fluidic chip is as a kind of novel analysis platform, has microminiaturization, automation, advantage such as integrated, convenient and quick, obtained to use widely in the environment measuring association area.Yet; Adopt disk shape chip; Utilize micro-processing technology to be used for analyzing in its surface preparation micro-structural and microchannel; Rely on centrifugal force to drive the sample microfluid, accomplish the centrifugal type microfludic chip analytical technology of a plurality of sample detection simultaneously, in the fast detecting related application field of oil and grease substantial breakthrough is not arranged as yet at present.
Summary of the invention
The purpose of this invention is to provide a kind of centrifugal type microfludic chip that detects oil and grease and preparation method thereof; It is characterized in that this chip is the centrifugal type microfludic chip of disk shape band micro-structural and microchannel; By multilayer be carved with micro-structural and the microchannel of micron level chip, form as the teflon film and the double-deck adhesive membrane involution of optical window; With the driving force of centrifugal force as the sample microfluid; Accomplishing the mixing of sample and extractant, extraction and separation process, is checkout gear with the infrared spectrometer, quantitatively or qualitatively detects on the chip oil and content of oil and grease in the sample.Micro-structural and microchannel comprise little sample cell, abstraction pool, detection cell, little valve, microchannel, microflute and micropore through the micro-processing technology preparation.The rotation platform of centrifuge aims at disk shape sample design, and the center is with sucker, draw-in groove or screw-nut fixed wafer shape centrifugal type microfludic chip.
For realizing above-mentioned purpose, the present invention adopts following operating procedure:
1. design and draw the micro-structural and the microchannel figure of each layer chip in the centrifugal type microfludic chip with computer aided design software;
2. pass through micro-processing technology in required micro-structural and the microchannel of each layer micro-fluidic chip substrate surface processing and preparing, comprise little sample cell, abstraction pool, detection cell, little valve, microchannel, microflute and micropore;
3. testing sample is put into abstraction pool;
4. utilize double-deck adhesive membrane, with each leafing core type micro-fluidic chip align, bonding, pressurizing and sealing, form centrifugal type microfludic chip;
5, behind the chip involution, in abstraction pool, add extractant;
6. startup centrifuge, testing sample mixes under centrifugal action, extracts and separate with extractant;
Sample treatment good after, on infrared spectrometer, detect extraction solution, obtain oil and content of oil and grease.
Among the present invention, the test sample that detects the centrifugal type microfludic chip of oil and grease can be water body example, also but pedotheque.
Among the present invention, the chip substrates that detects the centrifugal type microfludic chip of oil and grease can be PMMA, PC, PVC, COC, silicon chip, glass, copper, aluminium, stainless steel disk, also commercially available all kinds of CD CDs.
Among the present invention, detect the micro-structural and the microchannel of the centrifugal type microfludic chip of oil and grease and can mill quarter, laser ablation, LIGA technology, method of molding, pressure sintering, chemical attack preparation through numerical control, also available soft lithographic technique preparation.
Among the present invention, with double-deck adhesive membrane involution, double-deck adhesive membrane can be that power causes double-deck adhesive membrane, also common double faced adhesive tape film between each layer chip of the centrifugal type microfludic chip of detection oil and grease.
Among the present invention; Detect the width and the degree of depth that are connected the microchannel between abstraction pool and the detection cell of centrifugal type microfludic chip of oil and grease and be 380 μ m; The quartz capillary of inserting external diameter 375 μ m is as little valve; The internal diameter of quartz capillary can be 25,50,75,100 μ m, also can be 150 μ m.
Among the present invention, the standard oil sample that detects the centrifugal type microfludic chip of oil and grease is a benzene, and isohexane, three kinds of reagent of hexadecane by volume percentage (1: 1: 1.5) mixed preparing form.
Among the present invention; The centrifugal type microfludic chip that detects oil and grease need not any external pump and valve; Utilize centrifugal force to drive the sample microfluid; When extractant and sample mix extraction, centrifuge speed is 150~300 rev/mins, and extraction solution is 700~1000 rev/mins through the centrifugal rotational speed of quartz capillary.
Among the present invention, detecting the oil and the centrifugal type microfludic chip extraction oil of grease and the reagent of grease is tetrachloro-ethylene and carbon tetrachloride, and toxicity is littler than carrene and dimethyl sulfoxide (DMSO), has reduced the influence to environment.
Among the present invention, the centrifugal type microfludic chip that detects oil and grease adopts teflon film as the infrared optics form, and teflon film is difficult for by the organic extractant dissolving, and infrared light district transmitance is good, is easy to processing and fabricating.
The detection oil that the present invention proposes and the disk shape centrifugal type microfludic chip of grease; Can realize the parallel detection of a plurality of samples; Reduce the consumption of reagent and sample, simplified the pre-treatment of testing sample, shortened detection time; Have portable, economic, characteristics fast and efficiently, have a good application prospect in the association area of fast detecting oil and grease.
Description of drawings
Fig. 1. the nine layers of chip of the centrifugal type microfludic chip of detection oil and grease and the structural representation of one group of sample analysis detecting unit.
A. the 1st layer (bottom chip, involution is used), the 2nd layer of B. (double-deck adhesive membrane, bonding usefulness), the 3rd layer of (teflon film of C.; Optical window), the 4th layer of D. (double-deck adhesive membrane, bonding usefulness), the 5th layer of E. (middle master chip has micro-structural and microchannel); F. the 6th layer (double-deck adhesive membrane, bonding usefulness), the 7th layer of G. (teflon film, optical window); H. the 8th layer (double-deck adhesive membrane, bonding usefulness), the 9th layer of I. (the upper strata chip has sample holes and passage); J. the detection cell structural representation of one group of sample analysis detecting unit: 1) abstraction pool, 2), 3) quartz capillary, 4) ventilation microchannel and hole.
Specific embodiments
Embodiment 1
Design and draw the micro-structural and the microchannel figure of nine layers of chip of centrifugal type microfludic chip with computer aided design software.On three strata methyl methacrylate (PMMA) chips, prepare micro-structural and microchannel through numerical control CNC micro-machining system, clean each layer chip with running water, distilled water respectively, and with spots such as the residual fingerprint of ethanol wiping chip surface, oil stains.On two-layer teflon film and four layers of double faced adhesive tape film, with carving machine processing and preparing required micro-structural and microchannel.5mg pedotheque or standard oil sample are positioned in the abstraction pool, with nine layers of chip carefully align, bonding, pressurizing and sealing, process the centrifugal type microfludic chip that detects oil and grease.Centrifugal type microfludic chip is fixed on the centrifuge rotation platform through sucker, adds 100 μ L C from the sample hand-hole
2Cl
4, mix with 300 rev/mins rotating speeds, behind the extraction 10min, improve rotating speed to 1000 rev/min, extraction solution in the sample cell is all moved to detection cell, at 2855cm
-1The place carries out infrared spectrum to be detected, and obtains oil and content of oil and grease in the sample at last.
Embodiment 2
Design and draw the micro-structural and the microchannel figure of nine layers of chip of centrifugal type microfludic chip with computer aided design software.On three strata carbonic ester (PC) chips, prepare micro-structural and microchannel through numerical control CNC micro-machining system, clean each layer chip with running water, distilled water respectively at last, and with spots such as the residual fingerprint of ethanol wiping chip surface, oil stains.Cause on the adhesive membrane in two-layer teflon film and four layers of power, with carving machine processing and preparing required micro-structural and microchannel.5mL water sample or standard oil sample are positioned in the abstraction pool, with nine layers of chip carefully align, bonding, pressurizing and sealing, process the centrifugal type microfludic chip that detects oil and grease.Centrifugal type microfludic chip is fixed on the centrifuge rotation platform through draw-in groove, adds 100 μ L C from the sample hand-hole
2Cl
4, mix with 150 rev/mins rotating speeds, behind the extraction 10min, improve rotating speed to 700 rev/min, extraction solution in the sample cell is all moved to detection cell, at 2855cm
-1The place carries out infrared spectrum to be detected, and obtains oil and content of oil and grease in the sample at last.
Claims (10)
1. one kind is detected oil and centrifugal type microfludic chip of grease and preparation method thereof; It is characterized in that; This chip is the centrifugal type microfludic chip of disk shape band micro-structural and microchannel; By multilayer be carved with micro-structural and the microchannel of micron level chip, form as the teflon film and the double-deck adhesive membrane involution of optical window, with the driving force of centrifugal force, accomplish the mixing of testing sample and extractant, extraction and separation process as the sample microfluid; With the infrared spectrometer is checkout gear, quantitatively or qualitatively detects on the chip oil and content of oil and grease in the sample.Micro-structural and microchannel comprise little sample cell, abstraction pool, detection cell, little valve, microchannel, microflute and micropore through the micro-processing technology preparation.The rotation platform of centrifuge aims at disk shape sample design, and the center is with sucker, draw-in groove or screw-nut fixed wafer shape centrifugal type microfludic chip.
2. by described centrifugal type microfludic chip that detects oil and grease of claim 1 and preparation method thereof, it is characterized in that its making step is following:
1) with computer aided design software design with draw the micro-structural and the microchannel figure of each layer chip in the centrifugal type microfludic chip;
2) pass through micro-processing technology in required micro-structural and the microchannel of each layer micro-fluidic chip substrate surface processing and preparing, comprise little sample cell, abstraction pool, detection cell, little valve, microchannel, microflute and micropore;
3) testing sample is put into abstraction pool;
4) utilize double-deck adhesive membrane, with each leafing core type micro-fluidic chip align, bonding, pressurizing and sealing, form centrifugal type microfludic chip;
5) behind the chip involution, in abstraction pool, add extractant;
6) start centrifuge, testing sample mixes under centrifugal action, extracts and separate with extractant;
7) sample treatment good after, on infrared spectrometer, detect extraction solution, obtain oil and content of oil and grease.
3. by claim 1 or 2 described centrifugal type microfludic chips that detect oil and grease and preparation method thereof; It is characterized in that; The Core Feature device of this oil and grease detection chip is the centrifugal type microfludic chip that the disk shape has micro-structural and microchannel; The size of micro-structural and microchannel is a micron level, and the micro-processing method that mills quarter, laser ablation, LIGA technology, method of molding, pressure sintering, chemical attack, soft lithographic technique through numerical control is in the chip substrates surface preparation.Disk shape centrifugal type microfludic chip can be produced in batches, repeatedly utilization, flexible design and assembling.
4. by claim 1 or 2 described centrifugal type microfludic chips that detect oil and grease and preparation method thereof; It is characterized in that; This oil and grease detection chip adopt teflon film as the infrared optics form; Teflon film is difficult for by the organic extractant dissolving, and infrared light district transmitance is good, is easy to processing and fabricating.
5. by claim 1 or 2 described centrifugal type microfludic chips that detect oil and grease and preparation method thereof; It is characterized in that; This oil need not any external pump that is connected with the grease detection chip, and the sample microfluid is under centrifugal force drives, to mix with extraction agent, extract and separate.
6. by claim 1 or 2 described centrifugal type microfludic chips that detect oil and grease and preparation method thereof; It is characterized in that; This oil and grease detection chip need not the sample introduction pre-treatment; Sample pretreatment and detection be can on chip, directly accomplish, and oil and content of oil and grease in the sample detected simultaneously.
7. by claim 1 or 2 described centrifugal type microfludic chips that detect oil and grease and preparation method thereof; It is characterized in that; This oil and grease detection chip can be made many group micro-structurals and microchannel on chip piece; Constitute many group sample analysis detecting units, improve the parallel detectability of unit interval.
8. by claim 1 or 2 described centrifugal type microfludic chips that detect oil and grease and preparation method thereof; It is characterized in that; This oil need not any external valve that is connected with the grease detection chip; Little valve of control sample microfluidic flow is embedded in the microchannel by quartz capillary and constitutes, and regulates and control the flow regime of microfluid through the internal diameter of control quartz capillary.
9. by claim 1 or 2 described centrifugal type microfludic chips that detect oil and grease and preparation method thereof; It is characterized in that; This oil and grease detection chip are through the rotating speed of change centrifuge, make extract be transferred to detection cell from abstraction pool through quartz capillary, and the internal diameter of quartz capillary is more little; The sample microfluid is just big more through the required centrifugal force of this quartz capillary, and required centrifugal rotational speed is just high more.
10. by claim 1 or 2 described centrifugal type microfludic chips that detect oil and grease and preparation method thereof; It is characterized in that; The design of this oil and grease detection chip is ingenious, equipment simple, be easy to carry, Direct Sampling, need not little, the parallel detectability of sample treatment, sample and reagent dosage high, be prone to be connected with detector, the pre-treatment and the testing process of sample are accomplished on same chip, sample need not transfer, sample cross pollution probability is little; Satisfy the needs of micro-total analysis system development well, be with a wide range of applications in association area convenient, that detect oil and grease quickly and accurately.
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CN113893958A (en) * | 2021-12-10 | 2022-01-07 | 深圳市易瑞生物技术股份有限公司 | Centrifuge tube taking and placing method, device and computer readable storage medium |
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