CN103040729A - Chitosan gel for removing acne scars as well as preparation method and medical application thereof - Google Patents
Chitosan gel for removing acne scars as well as preparation method and medical application thereof Download PDFInfo
- Publication number
- CN103040729A CN103040729A CN2013100405997A CN201310040599A CN103040729A CN 103040729 A CN103040729 A CN 103040729A CN 2013100405997 A CN2013100405997 A CN 2013100405997A CN 201310040599 A CN201310040599 A CN 201310040599A CN 103040729 A CN103040729 A CN 103040729A
- Authority
- CN
- China
- Prior art keywords
- chitosan
- gel
- acidulant
- acne
- acetic acid
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Medicinal Preparation (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Abstract
The invention relates to chitosan gel for removing acne scars. The chitosan gel for removing acne scars is obtained by mixing chitosan with specific molecular weight and degree of deacetylation, glycerol, acidifier and purified water in a gelatinization manner and then sterilizing. The chitosan gel for removing acne scars, provided by the invention, has good adhesive power, uniform colour and lustre and stability, and has the advantages of good curative effect, safety, natural property, good biocompatibility and the like when used for treating acnes, pimples and seborrheic dermatitis and preventing acne scars. The invention also relates to a preparation method and application of the chitosan gel for removing acne scars.
Description
Technical field
The present invention relates to the medical product field, concrete, the present invention relates to a kind of chitosan gel rubber, it is the treatment for acne, skin sore, seborrheic dermatitis, and the generation etc. that is used for preventing Acne Scar, have the advantages such as good curative effect, safe, natural, good biocompatibility, the invention still further relates to its preparation method and medical applications.
Background technology
The external medical product of desirable Acne treatment and inhibition Acne Scar should have the functions such as good antimicrobial antiphlogistic, safety, biocompatibility, ease of use, will be easy to simultaneously storage, easy to use.
Chitosan (Chitosan), chemistry by name poly-(1,4)-2-amino-2-deoxidation-β-D-Glucose, that chitin partly or entirely takes off a kind of natural biological polymer that obtains behind the acetyl, it is the only macromolecule alkalescence polysaccharide that nature exists, be called again soluble chitin, Chitosan, chitosan, chitosan, have good biocompatibility, the characteristics such as abundant that have no side effect, easily are absorbed, originate, obtain widely development and application in the field such as synthetic of food industry, chemical industry, medical macromolecular materials.
Technology and the product of the gel take chitosan as main constituent are more, and China Patent Publication No. CN102626524A discloses a kind of take chitosan as main constituent, the chitosan anti-bacteria gel take poloxamer as thickening agent; China Patent Publication No. CN101507731A discloses a kind of chitosan anti-bacteria gel (film) take chitosan as main constituent take gelatin as thickening agent; China Patent Publication No. CN 102247616A discloses a kind of take chitosan as main constituent, the gel preparation take carbomer and Polyethylene Glycol as thickening agent.
Above-mentioned disclosed chitosan gel rubber, make chitosan and other macromolecular compound (such as carbomer, gelatin, Polyethylene Glycol or poloxamer etc.) compatibility, it mainly is the angle from thickening, tackify and film property, expectation obtains good gel delivery agent, but from the weak point aspect following three:
The first point, because chitosan just had good crosslinked adhesive reaction and gelling characteristics originally under sour environment, the adding of the polymer chemistry material that these biocompatibility are poor, hindered the cross-linking reaction of chitosan linear molecule, also reduced the effective content of chitosan, so that the content of chitosan is all less, generally all less than 3%, even less, make the gel character variation of product;
Second point, although the adding of these polymer substances has the effect of thickening agent, but the adhesion property variation of chitosan, human body self body fluid (such as perspiration, acne secretions, oils and fats, vaginal secretions etc.) under the effect, the adhesive capacity of chitosan and people's body surface diminishes, and is easy to run off from body surface, greatly reduce action time, so that therapeutic effect is had a greatly reduced quality;
Thirdly, for patients with acne is arranged, the adding of the material that these biocompatibility are poor tends to cause stimulation to wound surface, stickingly touches property injury and heterologous reaction, and the nursing of wound is caused adverse effect.
The invention provides a kind of new chitosan gel rubber, specifically a kind of anti-acne, the chitosan gel rubber of trace and preparation method thereof of dispelling, it only contains a kind of main constituent of chitosan, and has good viscosity, adhesiveness and stability.
Summary of the invention
The inventor studies discovery, affects the quality of anti-acne trace chitosan gel rubber of the present invention, and many factors are arranged, and direct influence factor wherein has following three factors:
The one, the molecular weight of chitosan and deacetylation, the chitosan that molecular weight is little, deacetylation is high, its anti-microbial property is better, but its gel viscosity and adhesiveness are poor;
The 2nd, when the pH value of gel, the pH value of gel were low, viscosity and adhesion diminished, and when the pH value of gel was higher, although preferably viscosity and adhesion are arranged, the less stable of gel can not form stable, transparent gel;
The 3rd, the content of chitosan in the gel, the content of chitosan is for viscosity and the adhesion of gel, affects greatlyr, and chitosan content is high, although the viscosity of gel and adhesion are better, gel is easy to occur layering and separates out the chitosan solid;
In addition, affect the indirect factor of anti-acne trace chitosan gel rubber quality of the present invention, prior have two factors:
The one, such medical product all is aseptic usually, all need to sterilize for Product Terminal, usually employing cobalt-60 irradiation is penetrated processing or is carried out high-temperature heat sterilization, after adopting cobalt-60 irradiation to penetrate processing or carrying out high-temperature heat sterilization, viscosity and the adhesion of chitosan gel rubber tend to variation, when especially adopting cobalt-60 irradiation to inject capable sterilization treatment, the dosage size of radiation is crucial influence factor;
The 2nd, the selection of acidulant, under the relative rigid condition of pH value of gel, with different acidulant, the gel after the terminal sterilization that obtains, its viscosity and adhesion, color and luster homogeneous, stability, abnormal smells from the patient have significantly difference, and be very large to the quality of anti-acne trace chitosan gel rubber of the present invention.
Based on above many directly and indirectly influence factors, the invention provides a kind of aseptic anti-acne trace chitosan gel rubber, contain chitosan 7%～12%, glycerol 5%～10%, acidulant and purified water, the viscosity-average molecular weight of wherein said chitosan be 4～100,000 and its deacetylation greater than 85%, described acidulant is the mixture of lactic acid, acetic acid or hydrochloric acid-acetic acid; And the viscosity of described gel after cobalt-60 irradiation is penetrated processing or carried out high-temperature heat sterilization is greater than 55Pa.s, and the pH value of described gel is 3.8～5.8.
Preferably, the present invention selects the mixture of hydrochloric acid-acetic acid as acidulant, adopt concentration be 36%～38% hydrochloric acid and glacial acetic acid take volume ratio as (1～5): 1 mixture is as acidulant, and especially selecting concentration is that 36%～38% hydrochloric acid and the mixture of glacial acetic acid take volume ratio as 5:1 are as acidulant.
Common, anti-acne trace chitosan gel rubber of the present invention adopts packing and the sealings such as plastic-aluminum flexible pipe, plastic tube, cillin bottle, aluminum pipe, is conducive to preserve and patient's use, and is preferred, uses the plastic-aluminum flexible pipe.
One skilled in the art will appreciate that chitosan is a kind of high molecular polymer, belong to the mixture category, the assay method of molecular weight is a lot, surveys relative molecular weight, i.e. viscosity-average molecular weight (M such as viscosity method
η); Low angle laser light scattering method check weighing average molecular weight (Mw); Size exclusion chromatography (SES) records the relative molecular weight of different component; Mass spectrography accurately records weight average molecular weight (Mw); In addition, also have end group measurement, ebulliometry, vapor-pressure osmometry.The characteristics such as wherein, viscosity method adopts Ubbelohde viscometer, and it is simple to have equipment, easy to operate are methods commonly used at present.Because chitosan is a kind of high molecular polymer, its molecular weight is its assembly average, and is common, represents with viscosity-average molecular weight.
Need to particularly point out and those skilled in the art can be understood that, chitosan molecule amount of the present invention, be often referred to the relative molecular weight of measuring with viscosimetry, although molecular weight of the present invention is the value of determining, for the chitosan of high molecular polymer, be actually relative molecular weight, be a value range, this value range is molecular weight determined value ± 10% normally, for example, molecular weight is 50,000 chitosan, and the molecular weight ranges of in fact this chitosan is 4.5～5.5 ten thousand; Molecular weight is 60,000 chitosan, and the molecular weight ranges of in fact this chitosan is 5.4～6.6 ten thousand; Molecular weight is 100,000 chitosan, and the molecular weight ranges of in fact this chitosan is 9～110,000, etc.
Concrete, the invention provides a kind of aseptic anti-acne trace chitosan gel rubber, it is characterized in that:
(1) contain viscosity-average molecular weight be 4～100,000 and its deacetylation greater than 85% chitosan 7%～12%, glycerol 5%～10%, acidulant and purified water, wherein said acidulant is the mixture of lactic acid, acetic acid or hydrochloric acid-acetic acid;
(2) viscosity of described gel after cobalt-60 irradiation is penetrated processing or carried out high-temperature heat sterilization is greater than 55Pa.s;
(3) pH value of described gel is 3.8～5.8.
Preferably, the invention provides a kind of aseptic anti-acne trace chitosan gel rubber, it is characterized in that:
(1) contain viscosity-average molecular weight be 5～80,000 and its deacetylation greater than 90% chitosan 8%～10%, glycerol 6%～8%, acidulant and purified water, described acidulant is the mixture of lactic acid, acetic acid or hydrochloric acid-acetic acid;
(2) viscosity of described gel after cobalt-60 irradiation is penetrated processing or carried out high-temperature heat sterilization is greater than 95Pa.s;
(3) pH value of described gel is 3.8～5.8.
Those skilled in the art should understand, chitosan of the present invention also comprises the derivant of chitosan, for example include but not limited to: carboxymethyl chitosan, chitosan hydrochlorate, chitosan lactate, chitosan acetate, chitosan sulfate fat, chitosan quaternary amine, Chitosan oligosaccharide, hyaluronic acid-like chitosan or chitin, etc.
The present invention further provides the preparation method of this anti-acne trace chitosan gel rubber, it is included in cleanliness factor is in 100,000 grades the environment, chitosan is added in the purified water, stir and the adding acidulant, regulate pH of latex gel to 3.8～5.8, preferred pH of latex gel to 4～5.5 of regulating, adding glycerol stirs, optionally can be heated to 35～90 ℃ stirs and is down to room temperature, degassed, filling and sealing, sterilization get final product, and wherein acidulant is the mixture of lactic acid, acetic acid or hydrochloric acid-acetic acid, and sterilization is usually adopted cobalt-60 irradiation to penetrate processing or carried out high-temperature heat sterilization.
Concrete, the preparation method of anti-acne trace chitosan gel rubber of the present invention, it is included in cleanliness factor is in 100,000 grades the environment, chitosan is added in the purified water, stir and the adding acidulant, regulate pH of latex gel to 3.8～5.8, adding glycerol stirs, optionally can be heated to 35～90 ℃ stirs and is down to room temperature, degassed, fill is in the plastic-aluminum flexible pipe, and sealing is further penetrated processing with cobalt-60 irradiation of 7.5～13KGy dosage, namely obtain anti-acne trace chitosan gel rubber of the present invention, wherein acidulant is lactic acid, the mixture of acetic acid or hydrochloric acid-acetic acid, preferred cobalt-60 irradiation dose is 9.5～12.5KGy, for example 10KGy, 10.5KGy, 11KGy, 11.5KGy, 12KGy etc.
Perhaps, concrete, the preparation method of anti-acne trace chitosan gel rubber of the present invention, it is included in cleanliness factor is in 100,000 grades the environment, chitosan is added in the purified water, stir and the adding acidulant, regulate pH of latex gel to 3.8～5.8, add glycerol and stir, optionally can be heated to 35～90 ℃ and stir and be down to room temperature, degassed, fill is in cillin bottle, and 121 ℃ of moist heat sterilization 10～15min are further used in sealing, namely obtain anti-acne trace chitosan gel rubber of the present invention, wherein acidulant is the mixture of lactic acid, acetic acid or hydrochloric acid-acetic acid.
The screening study of correlation technique factor is as follows in the anti-acne trace chitosan gel rubber of the present invention:
One, the selection of the molecular weight of chitosan and deacetylation
Selecting respectively viscosity-average molecular weight is 20,000,30,000,40,000,60,000,80,000,100,000,120,000,140,000 chitosan, making content is 7%, pH value is 4.5 gel, find that viscosity-average molecular weight is 20,000,30,000 chitosan gel rubber viscosity and adhesion are too small, molecular weight is that 120,000 chitosan gel rubber has chitosan to separate out when room temperature is placed 43 days (about 1.5 months), less stable, molecular weight is that 140,000 chitosan gel rubber can't be made clear gel, be suspendible shape mastic, so the selection viscosity-average molecular weight is 4～100,000 chitosan, preferred viscosity-average molecular weight is 6～80,000 chitosan;
Selecting respectively deacetylation is 75%, 80%, 85%, 90%, 95% chitosan, make content and be 7%, pH value is 4.5 gel, find that deacetylation is that dissolubility is bad in the preparation for 75%, 80% chitosan, and the room temperature placement had chitosan particle in 6 months and separates out, it is muddy to be that gel becomes, so select deacetylation greater than 85% chitosan.Show viscosity-average molecular weight be 4～100,000 and deacetylation have good stability greater than the gel of the present invention of 85% chitosan preparation.
Two, the selection of the pH value of gel
Selecting viscosity-average molecular weight is 80,000 chitosan, make content and be 7%, pH value is 2.5,3.5,3.8,4.5,5.5,6.0 gel, find when pH value less than 3.8 the time, viscosity and the adhesion of gel diminish, and the pH value of gel was greater than 6 o'clock, and the room temperature placement had chitosan particle in 6 months and separates out, that gel becomes muddiness, being attached to the pH value scope is ± 1, is 3.8～5.8 so select the pH value of gel, and the pH value that shows gel is 3.8～5.8 to have good stability.
Three, the selection of chitosan content in the gel
Select viscosity-average molecular weight to be respectively 40,000,60,000,80,000,100,000 chitosan, making respectively content is 4%, 5%, 6%, 7%, 8%, 10%, 12%, 13%, and pH value is 4.5 gel, find when content less than 7% the time, viscosity and the adhesion of gel are relatively poor, when content greater than 12% the time, can't make clear gel, be suspendible shape mastic, show that gel has good stability and adhesion when selecting chitosan content to be 7%～12%.
Four, the selection of acidulant
Use respectively hydrochloric acid, lactic acid, the mixture of acetic acid or hydrochloric acid-acetic acid is done acidulant, selecting viscosity-average molecular weight is 80,000 chitosan, making content is 7%, 8%, 9%, pH value is 4.5 gel, the gel of acidulant is done in discovery with hydrochloric acid, after penetrating processing with cobalt-60 irradiation or carrying out high-temperature heat sterilization, viscosity significantly descends, viscosity become the sterilization before 27%～32%, may be that hydrochloric acid belongs to strong acid, cobalt-60 irradiation penetrate process or when carrying out high-temperature heat sterilization the crosslinked action to chitosan gel rubber produce strong destruction so that viscosity declines to a great extent, so select lactic acid, the mixture of acetic acid or hydrochloric acid-acetic acid is done acidulant.
Five, the mixture of hydrochloric acid-acetic acid is done determining of acidulant
When doing acidulant with acetic acid, the gel that obtains has strong acetic acid stink, select the mixture of the different volumes proportioning formation of acetate and hydrochloride to do acidulant, can obtain cobalt-60 irradiation penetrate process or carry out high-temperature heat sterilization after viscosity change little, and the gel that does not have obvious acetic acid stink only has extremely slight acetic acid flavor.
Adopt concentration be 36%～38% hydrochloric acid and glacial acetic acid take volume ratio as (1～5): 1 mixture is as acidulant, in this scope, adopt the mixture acidulant of different volume proportions, the gel that obtains is transparent, good stability, after penetrating processing with cobalt-60 irradiation or carrying out high-temperature heat sterilization, viscosity changes less.
Preferably, the present invention selects the mixture of hydrochloric acid-acetic acid as acidulant, especially selecting concentration is that 36%～38% hydrochloric acid and the mixture of glacial acetic acid take volume ratio as 5:1 are as acidulant, the gel that obtains is transparent, good stability, room temperature was placed 6 months, it is consistent with 0 month that the viscosity of gel, pH value, color and luster homogeneous, abnormal smells from the patient all keep, gel shows good stability, show the mixture of selecting hydrochloric acid-acetic acid as acidulant, especially selecting concentration is that 36%～38% hydrochloric acid and the mixture of glacial acetic acid take volume ratio as 5:1 are as acidulant.
Six, viscosity determines
Gel of the present invention is the external preparation for skin administration, and this class medicine tends to run off from skin very soon under the effect of acne secretions (such as perspiration, oils and fats etc.), so that the retention time of gel on acne is shorter, greatly affects therapeutic effect.
Make external model and carry out gel adhesion test of the present invention.Get commercially available Radix Polygalae Crotalarioidis, Radix Polygalae Crotalarioidis is turned over, the oil of the inside and soil are all plucked totally, crumple 2～3 times with vinegar and Alumen, again with flushing with clean water for several times, sliced with the rectangle that is cut into long 6cm, wide 2cm, blot water and the residual attached oils and fats on surface with filter paper, be weighed as M
0Be fixed on glass, the large intestine inner surface outwardly, tilt 30 °, smear the gel of 3g different viscosities, per five minutes with aerosol apparatus to gel surface spray 0.5ml normal saline, continuous spraying 10 times, spray the gel that takes off Radix Polygalae Crotalarioidis section and surface adhesion thereof behind five minutes after the 10th time, adsorbed to walk gently the water on surface with filter paper, be weighed as M
1, then the adhesive capacity of gel is
Get different viscosities 5Pa.s of the present invention, 25Pa.s, 35Pa.s, 55Pa.s, 75Pa.s, 95Pa.s, 150Pa.s chitosan gel rubber, according to above-mentioned external model, measure its adhesive capacity W, discovery viscosity is 5Pa.s, 25Pa.s and the W value of 35Pa.s is all less than 10%, viscosity is 55Pa.s, 75Pa.s, 95Pa.s and the W value of 105Pa.s is all greater than 25%, respectively 28.7%, 39.2%, 47.7%, 52.0%, 55.9%, show good adhesiveness, show that adhesion is better, show that viscosity has good adhesion greater than the chitosan gel rubber of the present invention of 55Pa.s.
Seven, sterilising conditions determines
In cleanliness factor is 100,000 grades environment, the gel for preparing different chitosan content, penetrate processing or moist heat sterilization by cobalt-60 irradiation, find all gels cobalt-60 irradiation of 13KGy dosage penetrate process or with 121 ℃ of moist heat sterilization 10～15min after, carry out immediately the bacterium inspection, result after culture period finishes is aseptic, and after cobalt-60 irradiation of 7.5 KGy dosage is penetrated processing, although partial gel carries out the bacterium inspection immediately, after finishing, culture period shows the bacterium survival, but after placing 3 days, carry out the bacterium inspection, result after the inspection culture period finishes is aseptic, may be the basic deactivation of antibacterial after cobalt-60 irradiation is penetrated processing, under the antibacterial action of gel itself, make its thorough deactivation.Show in cleanliness factor is 100,000 grades environment, prepare the gel of different chitosan content, through cobalt-60 irradiation of 7.5～13KGy dosage penetrate process or with 121 ℃ of moist heat sterilization 10～15min after can obtain aseptic gel.
The non-antibiotic class antibacterial of a kind of natural, safety and environmental protection of chitosan, have good antiinflammatory, antibacterial action, propionibacterium, staphylococcus aureus, escherichia coli, Candida albicans are had obvious inhibitory action, and gel is applied in the generation that can promote the acne healing behind the acne skin ulcer face and suppress Acne Scar.
Further, chitosan gel rubber of the present invention is as the direct administration of skin, in preparation Acne treatment, skin sore, seborrheic dermatitis and the application of medicine that is used for preventing the generation of Acne Scar.
The specific embodimentIn implementation process of the present invention, various embodiments that those of ordinary skills produce on the basis that does not depart from the scope of the present invention with spirit and modify apparent and be to carry out easily, the invention is not restricted to the scope of specific embodiment as herein described, really, various modification of the present invention is except those that narrated are to be understood by those skilled in the art from the narration of front.Come the present invention done further specifying by the following examples, but do not represent the embodiment limitation of the present invention.
Embodiment 1, anti-acne trace chitosan gel rubber and preparation method thereof
Prescription: chitosan 10g (viscosity-average molecular weight is 60,000, deacetylation 〉=90%)
Glycerol 8g
37% hydrochloric acid and glacial acetic acid mixture (V:V=5:1) are an amount of
Purified water adds to 100g.
Preparation method: in cleanliness factor is 100,000 grades environment, get the 80ml purified water, the molecular weight that adds recipe quantity is 60,000 chitosan, stirring also adds an amount of hydrochloric acid-acetic acid mixed solution, make reactant form gel, and the pH value that makes gel is 4.2～4.5, adding glycerol stirs, degassed, fill is further penetrated sterilization treatment with cobalt-60 irradiation of 10～11KGy dosage in the sealing of plastic-aluminum flexible pipe, namely gets anti-acne trace chitosan gel rubber of the present invention, viscosity is 152Pa.s, and gel does not almost have the acetic acid flavor.
Embodiment 2, anti-acne trace chitosan gel rubber and preparation method thereof
Prescription: chitosan 9g (viscosity-average molecular weight is 50,000, deacetylation 〉=95%)
Glycerol 8g
37% hydrochloric acid and glacial acetic acid mixture (V:V=5:1) are an amount of
Purified water adds to 100g.
Preparation method: in cleanliness factor is 100,000 grades environment, get the 80ml purified water, the molecular weight that adds recipe quantity is 50,000 chitosan, stirring also adds an amount of hydrochloric acid-acetic acid mixed solution, make reactant form gel, and the pH value that makes gel is 4.2～4.5, adding glycerol stirs, degassed, fill is further penetrated sterilization treatment with cobalt-60 irradiation of 10～11KGy dosage in the sealing of plastic-aluminum flexible pipe, namely gets anti-acne trace chitosan gel rubber of the present invention, viscosity is 91Pa.s, and gel does not almost have the acetic acid flavor.
Embodiment 3, anti-acne trace chitosan gel rubber and preparation method thereof
Prescription: chitosan 7g (viscosity-average molecular weight is 90,000, deacetylation 〉=85%)
Glycerol 8g
Lactic acid is an amount of
Purified water adds to 100g.
Preparation method: in cleanliness factor is 100,000 grades environment, get the 80ml purified water, the molecular weight that adds recipe quantity is 90,000 chitosan, stirs and adds an amount of lactic acid, make reactant form gel, and the pH value that makes gel is 4.0～4.5, adds glycerol and stirs, and is degassed, fill seals in the plastic-aluminum flexible pipe, further penetrate sterilization treatment with cobalt-60 irradiation of 8～9.5KGy dosage, namely get anti-acne trace chitosan gel rubber of the present invention, viscosity is 79 Pa.s; Perhaps degassed rear fill is in cillin bottle, and sealing further with 121 ℃ of sterilization 15min, namely obtains anti-acne trace chitosan gel rubber of the present invention, and viscosity is 81Pa.s.As seen, adopt different sterilizing methods, the aseptic gel that obtains, its viscosity approaches.
Embodiment 4, anti-acne trace chitosan gel rubber and preparation method thereof
Prescription: chitosan 8g (viscosity-average molecular weight is 80,000, deacetylation 〉=90%)
Glycerol 9g
Lactic acid is an amount of
Purified water adds to 100g.
Preparation method: in cleanliness factor is 100,000 grades environment, get the 80ml purified water, the molecular weight that adds recipe quantity is 80,000 chitosan, stirs and adds an amount of lactic acid, make reactant form gel, and the pH value that makes gel is 4～4.5, adds glycerol and stirs, and is degassed, fill seals in the plastic-aluminum flexible pipe, further penetrate sterilization treatment with cobalt-60 irradiation of 9～10KGy dosage, namely get anti-acne trace chitosan gel rubber of the present invention, viscosity is 87Pa.s.
Embodiment 5, anti-acne trace chitosan gel rubber and preparation method thereof
Prescription: chitosan 9g (viscosity-average molecular weight is 60,000, deacetylation 〉=90%)
Glycerol 8g
Acetic acid is an amount of
Purified water adds to 100g.
Preparation method: in cleanliness factor is 100,000 grades environment, get the 80ml purified water, the molecular weight that adds recipe quantity is 60,000 chitosan, stirring also adds an amount of vinegar milk acid, make reactant form gel, and the pH value that makes gel add glycerol and stir 4.5～5, degassed, fill is further penetrated sterilization treatment with cobalt-60 irradiation of 9～10KGy dosage in the sealing of plastic-aluminum flexible pipe, namely gets anti-acne trace chitosan gel rubber of the present invention, viscosity is 109Pa.s, and gel has the acetic acid stink.
Embodiment 6, anti-acne trace chitosan gel rubber and preparation method thereof
Prescription: chitosan 10g (viscosity-average molecular weight is 50,000, deacetylation 〉=90%)
Glycerol 8g
Lactic acid is an amount of
Purified water adds to 100g.
Preparation method: in cleanliness factor is 100,000 grades environment, get the 80ml purified water, the molecular weight that adds recipe quantity is 50,000 chitosan, stirs and adds an amount of lactic acid, make reactant form gel, and the pH value that makes gel is 4.5～5, adds glycerol and stirs, and is degassed, fill seals in the plastic-aluminum flexible pipe, further penetrate sterilization treatment with cobalt-60 irradiation of 10～11KGy dosage, namely get anti-acne trace chitosan gel rubber of the present invention, viscosity is 141Pa.s.
Embodiment 7, anti-acne trace chitosan gel rubber and preparation method thereof
Prescription: chitosan 11g (viscosity-average molecular weight is 40,000, deacetylation 〉=95%)
Glycerol 9g
Lactic acid is an amount of
Purified water adds to 100g.
Preparation method: in cleanliness factor is 100,000 grades environment, get the 80ml purified water, the molecular weight that adds recipe quantity is 40,000 chitosan, stirs and adds an amount of lactic acid, make reactant form gel, and the pH value that makes gel is 4.5～5, adds glycerol and stirs, and is degassed, fill seals in the plastic-aluminum flexible pipe, further penetrate sterilization treatment with cobalt-60 irradiation of 10～11KGy dosage, namely get anti-acne trace chitosan gel rubber of the present invention, viscosity is 177Pa.s.
Embodiment 8,
The viscosity of the gel of embodiment 1～7 and adhesion, color and luster homogeneous, stability all show well; And propionibacterium, staphylococcus aureus, escherichia coli, Candida albicans are had obvious inhibiting bacteriostasis rate all greater than 85%, especially for the bacteriostasis rate of Candida albicans greater than 93%, demonstrate stronger antibiotic property; As the direct administration of skin, in preparation Acne treatment, skin sore, seborrheic dermatitis and the application of medicine that is used for preventing the generation of Acne Scar.
Claims (9)
1. aseptic anti-acne trace chitosan gel rubber is characterized in that:
(1) contain viscosity-average molecular weight be 4～100,000 and its deacetylation greater than 85% chitosan 7%～12%, glycerol 5%～10%, acidulant and purified water, wherein said acidulant is the mixture of lactic acid, acetic acid or hydrochloric acid-acetic acid;
(2) viscosity of described gel after cobalt-60 irradiation is penetrated processing or carried out high-temperature heat sterilization is greater than 55Pa.s;
(3) pH value of described gel is 3.8～5.8.
2. aseptic anti-acne trace chitosan gel rubber is characterized in that:
(1) contain viscosity-average molecular weight be 5～80,000 and its deacetylation greater than 90% chitosan 8%～10%, glycerol 6%～8%, acidulant and purified water, described acidulant is the mixture of lactic acid, acetic acid or hydrochloric acid-acetic acid;
(2) viscosity of described gel after cobalt-60 irradiation is penetrated processing or carried out high-temperature heat sterilization is greater than 95Pa.s;
(3) pH value of described gel is 3.8～5.8.
3. claim 1 or anti-acne trace chitosan gel rubber claimed in claim 2, it is characterized in that described acidulant be concentration be 36%～38% hydrochloric acid and glacial acetic acid take volume ratio as (1～5): 1 mixture.
4. claim 1 or anti-acne trace chitosan gel rubber claimed in claim 2 is characterized in that described acidulant is that concentration is 36%～38% hydrochloric acid and the mixture of glacial acetic acid take volume ratio as 5:1.
5. claim 1 or anti-acne trace chitosan gel rubber claimed in claim 2, wherein said chitosan also comprises: carboxymethyl chitosan, chitosan hydrochlorate, chitosan lactate, chitosan acetate, chitosan sulfate fat, chitosan quaternary amine or Chitosan oligosaccharide.
6. the preparation method of claim 1 or anti-acne trace chitosan gel rubber claimed in claim 2, it is included in cleanliness factor is in 100,000 grades the environment, chitosan is added in the purified water, stir and the adding acidulant, regulate pH of latex gel to 3.8～5.8, adding glycerol stirs, optionally being heated to 35～90 ℃ stirs and is down to room temperature, degassed, filling and sealing, sterilization get final product, wherein acidulant is the mixture of lactic acid, acetic acid or hydrochloric acid-acetic acid, and sterilization is adopted cobalt-60 irradiation to penetrate processing or carried out high-temperature heat sterilization.
7. the preparation method of claim 1 or anti-acne trace chitosan gel rubber claimed in claim 2, it is included in cleanliness factor is in 100,000 grades the environment, chitosan to be added in the purified water, stirs also to add acidulant, regulate pH of latex gel to 3.8～5.8, add glycerol and stir, optionally can be heated to 35～90 ℃ and stir and be down to room temperature, degassed, fill is in the plastic-aluminum flexible pipe, sealing is further penetrated processing with cobalt-60 irradiation of 7.5～13KGy dosage, and be get final product.
8. the preparation method of claim 1 or anti-acne trace chitosan gel rubber claimed in claim 2, it is included in cleanliness factor is in 100,000 grades the environment, chitosan to be added in the purified water, stirs also to add acidulant, regulate pH of latex gel to 3.8～5.8, add glycerol and stir, optionally can be heated to 35～90 ℃ and stir and be down to room temperature, degassed, fill is in cillin bottle, sealing is further used 121 ℃ of moist heat sterilization 10～15min, and be get final product.
9. claim 1 or anti-acne trace chitosan gel rubber claimed in claim 2 be as the direct administration of skin, in preparation Acne treatment, skin sore, seborrheic dermatitis and the application of medicine that is used for preventing the generation of Acne Scar.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2013100405997A CN103040729A (en) | 2013-02-03 | 2013-02-03 | Chitosan gel for removing acne scars as well as preparation method and medical application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2013100405997A CN103040729A (en) | 2013-02-03 | 2013-02-03 | Chitosan gel for removing acne scars as well as preparation method and medical application thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN103040729A true CN103040729A (en) | 2013-04-17 |
Family
ID=48053743
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2013100405997A Pending CN103040729A (en) | 2013-02-03 | 2013-02-03 | Chitosan gel for removing acne scars as well as preparation method and medical application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103040729A (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103877555A (en) * | 2014-04-16 | 2014-06-25 | 广西信业生物技术有限公司 | Chitosan cicatrix biological medical adhesive and preparation method thereof |
| CN105250153A (en) * | 2015-10-30 | 2016-01-20 | 石道林 | Cream for eye skin care |
| CN108434064A (en) * | 2018-06-05 | 2018-08-24 | 广州好迪集团有限公司 | Have both the skin care item of skin anti-inflammatory Eradicates acnes and repair function |
| CN109731021A (en) * | 2019-03-20 | 2019-05-10 | 武汉市思泰利医疗器械发展有限公司 | It is a kind of for treat repair stoma skin aquagel and preparation method thereof |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102818744A (en) * | 2012-08-24 | 2012-12-12 | 福州乾正药业有限公司 | Method for measuring chitosan content in preparation |
-
2013
- 2013-02-03 CN CN2013100405997A patent/CN103040729A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102818744A (en) * | 2012-08-24 | 2012-12-12 | 福州乾正药业有限公司 | Method for measuring chitosan content in preparation |
Non-Patent Citations (1)
| Title |
|---|
| 李晶等: "以芦荟、壳聚糖为原料制备面膜", 《河南化工》, no. 8, 31 December 2004 (2004-12-31), pages 19 - 20 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103877555A (en) * | 2014-04-16 | 2014-06-25 | 广西信业生物技术有限公司 | Chitosan cicatrix biological medical adhesive and preparation method thereof |
| CN105250153A (en) * | 2015-10-30 | 2016-01-20 | 石道林 | Cream for eye skin care |
| CN108434064A (en) * | 2018-06-05 | 2018-08-24 | 广州好迪集团有限公司 | Have both the skin care item of skin anti-inflammatory Eradicates acnes and repair function |
| CN109731021A (en) * | 2019-03-20 | 2019-05-10 | 武汉市思泰利医疗器械发展有限公司 | It is a kind of for treat repair stoma skin aquagel and preparation method thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103040728A (en) | Chitosan gel for women as well as preparation method and medical application thereof | |
| Liakos et al. | Controlled antiseptic release by alginate polymer films and beads | |
| Szymańska et al. | Stability of chitosan—a challenge for pharmaceutical and biomedical applications | |
| CN104257639B (en) | A kind of liniment for Wound treating and preparation method thereof | |
| CN105107008B (en) | A kind of hydroxyl butyl chitosan/oxidized sodium alginate/nano silver composite hydrogel dressing patch | |
| TWI233801B (en) | Ophthalmic compositions containing galactomannan polymers and borate | |
| CN105816911A (en) | Repairing gel containing growth factors and preparation method of repairing gel | |
| CN107007876A (en) | Hyaluronic acid medical aseptic dressing and preparation method thereof | |
| CN102755662B (en) | Medical gel film prepared by compounding chitosan and preparation method of same | |
| CN103263434B (en) | Anti-adhesion liquor for wound washing and surgery and preparation method and application of anti-adhesion liquor | |
| CN107412847A (en) | A kind of Wound dressing of liquid form and preparation method thereof | |
| CN105380924B (en) | A kind of chitosan oligosaccharide capsule agent and preparation method thereof | |
| CN104902954B (en) | Wound care products with superacid composite | |
| CN103830737B (en) | Functional starch matrix Capsules | |
| CN103040729A (en) | Chitosan gel for removing acne scars as well as preparation method and medical application thereof | |
| EA034053B1 (en) | Pharmaceutical compositions comprising collagen and sodium hyaluronate | |
| CN106178111B (en) | A kind of gel filled preparation of rhinology Thermo-sensitive and preparation method thereof and the application in nasal surgery | |
| KR20160146259A (en) | Method for preparing hydro gel containing bio cellulose | |
| CN103463125B (en) | Gel preparation for treating gynecological diseases and preparation method thereof | |
| CN107149688A (en) | The sterile production method of hyalomitome acids product | |
| CN103211753A (en) | Gel matrix | |
| CN111973801B (en) | Hydrogel for anti-inflammatory repair and preparation method thereof | |
| CN103446177B (en) | Cleansing solution for curing gynecological disease and preparation method thereof | |
| CN100540066C (en) | Medical chitosan dressing and production method thereof | |
| CN105107018B (en) | A kind of preparation method of sterile injection material |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20130417 |