CN103039539A - Compound preparation for resisting melon vegetables plant virus disease, as well as preparation method and application of compound preparation - Google Patents
Compound preparation for resisting melon vegetables plant virus disease, as well as preparation method and application of compound preparation Download PDFInfo
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Abstract
The invention provides a compound preparation for resisting melon vegetables plant virus disease, as well as a preparation method and an application of the compound preparation. The preparation comprises 40-90% by weight of effective constituent consisting of plant extract and chemical pesticide, and the balance being agricultural pharmacology permitted and acceptable general auxiliary materials, wherein the effective constituent consists of the following components in percentage by weight: 5-50% of pastel extract, 5-50% of garlic extract, 2-40% of albizzia julibrissin extract, 0.01-10% of imidacloprid and 0.01-5% of antitoxic Feng. The compound preparation provided by the invention can resist melon vegetables plant virus diseases, consists of plant extract including agricultural wastes and pesticide mixture, is high in efficiency, low in residue and low in production cost, and is capable greatly reducing the usage amount of chemical pesticides.
Description
Technical field
The present invention relates to a kind of anti-plant virus agent of technical field of pesticide, preparation and the application of the concrete a kind of compound that relates to a kind of plant extracts for the anti-gourd vegetables viroses of plant and agriculture chemical compounding.
Background technology
The viroses of plant are the important diseases of a class that harm the crops, and, control difficulty great because of its harm has the title of plant cancer.Bring about great losses to agricultural, the loss of global annual crops is up to multi-million dollar throughout the year.In recent years, the viroses of plant endanger seriously day by day, especially to economic crops.Picture is such as the gourd, fruit and vegetable class plant such as pumpkin, custard squash, fragrant sponge gourd and flowers etc.The gourd vegetables plant can present more typical virus disease symptom after dying virus disease being arranged, shrinkage, deformity in various degree appears in incidence of leaf, the blade variable color, produce floral leaf, spot, arteries and veins band and yellow, and on blade, also present necrotic plaque and arteries and veins necrosis, at the downright bad bar of the normal appearance in the surface of stem and root, in addition, sugar-preserved gourd presents dark green and light green alternate pattern, surface irregularity, sugar-preserved gourd deformity.Picture is such as main gourd vegetables planting base-Qingpu District, Shanghai, plant and be implanted with a large amount of gourd vegetables such as fragrant sponge gourd, pumpkin and custard squash, cultivated area is up to 550 mu, and serious virus disease occured in this planting base in 2010, caused a large amount of gourd vegetables leaf-shrinkages, the melon and fruit deformity, in addition, this viroid disease spread speed is very fast, and the incidence of disease is almost up to 100%, greatly reduce like this Quality and yield of gourd vegetables, make plantation peasant household suffer tremendous economic loss.
In order effectively to control the gourd vegetables viroses of plant; researchers have adopted various prophylactico-therapeutic measuress such as shift of crops, breeding for disease resistance, prevention and control to pass virus mediator, attenuated strain cross protection etc.; also carried out the development of chemical agent and natural, synthetic anti-gourd vegetables plant virus agent; but up to the present; the medicament kind that is applied to gourd vegetables phytopathy murder by poisoning control is also few; mainly be morpholine biguanidine hydrochloride, crystal aerugo, octicin solution and mixed agent thereof; and effect is all undesirable, and dosage and cost constantly increase.In addition, chemical pesticide uses the problem such as caused a series of environment to cause people's great attention in a large number, for this reason, develops a kind of novel gourd vegetables Control of Plant Virus Disease medicament combination and seems very urgent.Wherein, from plant, seek the material with antiviral activity, become an important channel of current novel anti-gourd vegetables plant virus agent initiative.In addition, arbitrarily abandoning of current a large amount of agricultural wastes, not only caused the idle and waste of ample resources, but also caused certain burden to environment, therefore, the present invention utilizes agricultural wastes and chemical pesticide composite, when guaranteeing drug effect, greatly reduce the use of chemical pesticide, and realized the recycling of agriculture waste resource, fully idle resource is brought into play its due society and economy and be worth, realized agriculture sustainable development.
Summary of the invention
For the prior art above shortcomings, the object of the invention is to provide agricultural wastes have the anti-gourd vegetables viroses of plant at interior plant extracts and agriculture chemical compounding compound preparation and preparation and the application that include that a kind of efficient, low-residual, production cost are low, can greatly reduce the chemical pesticide usage amount.
According to an aspect of the present invention, a kind of compound preparation of the anti-gourd vegetables viroses of plant is provided, be active ingredient (40%~90%) by plant extracts and chemical pesticide, all the other are to allow in the agricultural chemicals to use and the conventional auxiliary material of acceptable (10%~60%) composition, and the weight percentage of described active ingredient component and shared active ingredient total amount is: the blue extract 5~50% of Song, garlic P.E 5~50%, albizzia julibrissin extract 2~40%, Imidacloprid 0.01~10%, antitoxin rich 0.01~5%.
Described Song blue (Isatis tinctoria L.) takes from the blue stem of Song, leaf, flower or its mixture.The blue extract of described Song is to clay into power after the blue oven dry of Song, again through lixiviate, to filter, and reduced pressure concentration obtains.
Described garlic (Allium sativum L.) is taken from stem, the leaf of discarding after garlic is pruned or gathers in the crops; Described garlic P.E is with claying into power after the garlic oven dry, again through lixiviate, filters, and reduced pressure concentration obtains.
Described silk tree (Albizia julibrissin D.) is taken from discarded branch, leaf or the root that silk tree is pruned or naturally comes off; Described albizzia julibrissin extract is with claying into power after the silk tree oven dry, again through lixiviate, filters, and reduced pressure concentration obtains.
Described Imidacloprid and the antitoxin rich commercial product that is.Wherein Imidacloprid generalization formal name used at school is called 1-(6-chloropyridine-3-pyridylmethyl)-N-Nitroimidazoline-2-base amine, antitoxin rich be 0.5% mushroom derived protein amylase.
Described conventional auxiliary material refers to promote extract dissolving or the solubilizer (tween, polyethylene glycol, neopelex, Potassium dodecylbenzenesulfonate etc.), emulsifier (odium stearate, lauryl sodium sulfate, APEO etc.) and/or the suspending agent that suspend, synergist (Tea Saponin etc.), bleeding agent (emulsifier BP etc.) etc.
According to another aspect of the present invention, the preparation method of the compound preparation of the above-mentioned anti-gourd vegetables viroses of plant is provided, be specially: respectively with the blue stem of Song, leaf, flower or its mixture and garlic are pruned or the rear discarded stem of results, leaf, the discarded branch that silk tree is pruned or naturally comes off, leaf, flower or its mixture, clay into power after the oven dry, with ethanolic solution as extraction solvent, lixiviate, filter, filtrate adopts the method for reduced pressure concentration to obtain respectively medicinal extract, and then add Imidacloprid and antitoxin rich, and add at last conventional auxiliary material, make the compound preparation of the anti-gourd vegetables viroses of plant of the present invention.
Preferably, described oven dry refers to: 30-50 ℃ of lower oven dry.
Preferably, described powder refers to: 20-40 purpose granulated powders.
Preferably, described ethanol refers to ethanolic solution or the absolute ethyl alcohol of 10%-95%v/v, and its consumption is 10-20 times of powder quality.
Preferably, described lixiviate, its time is 1-7 days.
Preferably, described reduced pressure concentration refers to: the crude extract that obtains after the lixiviate is vacuumized decompression to-0.1 atmospheric pressure and places 30-80 ℃ of lower water-bath, make crude extract simmer down to medicinal extract.
According to a further aspect of the invention, provide the application of the compound preparation of the above-mentioned anti-gourd vegetables viroses of plant, refer to: under stirring at normal temperature, water is added in the compound preparation, its active ingredient that is diluted to preparation is accounted for spray after the concentration of 0.5-5wt% and use.
It is sick that the compound preparation of the anti-gourd vegetables viroses of plant of the present invention can be applied in the fragrant sponge gourd of control, pumpkin, custard squash etc. gourd vegetables Plant virus.
Compared with prior art, the present invention has following beneficial effect:
(1) synergy can strengthen the Antiphytoviral effect on the one hand between the primary raw material of composition product of the present invention, on the other hand because the acting in conjunction of the active ingredient of the different mechanisms of action can delay the pesticide resistance generation of medicament; In addition, can also greatly reduce the use of chemical pesticide.
(2) raw material be easy to get, process simple, low for equipment requirements, extremely be beneficial to suitability for industrialized production, and the solvent after reclaiming also can reuse.Three Plants raw materials have plantation in the most of place of China, and garlic of the present invention and silk tree all belong to agricultural wastes, and therefore, the present invention has realized the recycling of resource, has avoided secondary pollution, is conducive to the sustainable development of agricultural.
(3) the main active ingredient of the compound preparation of these anti-gourd vegetables viroses of plant derives from natural plant extracts; be easy under field conditions (factors) decompose; to environment and ecosystem safety on every side; compare with single chemical pesticide; pollution to environment reduces greatly, and tool has certain effect for preserving the ecological environment.
Embodiment
The present invention is described in detail below in conjunction with specific embodiment.Following examples will help those skilled in the art further to understand the present invention, but not limit in any form the present invention.Should be pointed out that to those skilled in the art, without departing from the inventive concept of the premise, can also make some distortion and improvement.These all belong to protection scope of the present invention.
Colonize in fragrant sponge gourd with antivirotic control of the present invention, pumpkin, virus disease on the custard squash plant is example, illustrate that the present invention prevents and treats the effect of the gourd vegetables viroses of plant, in following examples, conventional auxiliary material is take tween as example, should be understood that conventional auxiliary material can adopt other can promote extract dissolving or the solubilizer (tween that suspends equally, polyethylene glycol, neopelex, Potassium dodecylbenzenesulfonate etc.), emulsifier (odium stearate, lauryl sodium sulfate, APEO etc.) and/or suspending agent, synergist (Tea Saponin etc.), bleeding agent (emulsifier BP etc.) etc.Imidacloprid and antitoxin rich the purchase in Shanghai City Sapporo town Agricultural Materials Company.Other unaccounted conditions operate according to general common technology.
Embodiment 1
The compound preparation of the anti-gourd vegetables viroses of plant that active ingredient 80% and conventional auxiliary material 20% form
With discarded stem, leaf after the blue stem of Song, leaf, flower or its mixture and garlic pruning or the results, branch, leaf, flower or its mixture discarded that silk tree is pruned or naturally comes off are clayed into power after the 30-50 ℃ of lower oven dry.Take by weighing respectively the blue stem of Song, leaf, flower or its mixture and garlic are pruned or the rear discarded stem of results, leaf, the discarded branch that silk tree is pruned or naturally comes off, leaf, each 200g of powder (20 order particle) of flower, ethanolic solution 500ml with 95% floods 2d in room temperature, filter, the filtrate decompression concentration and recovery solvent of getting three Plants obtains extracting medicinal extract, then mix these three kinds and extract medicinal extract (wherein, the blue medicinal extract that extracts of Song occupies effect composition total amount 14.95%, garlic 40%, silk tree 40%), 0.05% Imidacloprid of rear adding active ingredient total amount and 5.0% antitoxin rich, and then adding weight accounts for the tween of whole antivirus compound formulation 20%, stir according to common process, it is mixed, make the compound preparation of the anti-gourd vegetables viroses of plant.And then dilute with water, being mixed with active ingredient concentration is the compound preparation of the anti-gourd vegetables viroses of plant of 2wt%.This compound preparation is carried out field control effectiveness test to fragrant sponge gourd virus disease.The fragrant sponge gourd virus disease initial stage of origination in the field, with the medicament for preparing, foliar spray, statistical results after 7 days, preventive effect reaches 60.4%.
Embodiment 2
The compound preparation of the anti-gourd vegetables viroses of plant that active ingredient 70% and conventional auxiliary material 30% form
Take by weighing respectively the blue stem of Song, leaf, flower or its mixture and garlic are pruned or the rear discarded stem of results, leaf, the discarded branch that silk tree is pruned or naturally comes off, leaf, each 200g of powder (20 order particle) of flower, ethanolic solution 1000ml with 10% floods 4d in room temperature, filter, the filtrate decompression concentration and recovery solvent of getting three Plants obtains extracting medicinal extract, then mix these three kinds and extract medicinal extract (wherein, the blue medicinal extract that extracts of Song occupies effect composition total amount 35%, garlic 24.94%, silk tree 40%), 0.01% Imidacloprid of rear adding active ingredient total amount and 0.05% antitoxin rich, and then adding weight accounts for the tween of whole antivirus compound formulation 30%, stir according to common process, it is mixed, make the compound preparation of the anti-gourd vegetables viroses of plant.And then dilute with water, being mixed with active ingredient concentration is the compound preparation of the anti-gourd vegetables viroses of plant of 1wt%.This compound preparation is carried out field control effectiveness test to squash virus.At field squash virus initial stage of origination, with the medicament for preparing, foliar spray, statistical results after 7 days, preventive effect reaches 51.2%.
Embodiment 3
The compound preparation of the anti-gourd vegetables viroses of plant that active ingredient 90% and conventional auxiliary material 10% form
Take by weighing respectively the blue stem of Song, leaf, flower or its mixture and garlic are pruned or the rear discarded stem of results, leaf, the discarded branch that silk tree is pruned or naturally comes off, leaf, each 300g of powder (20 order particle) of flower, ethanolic solution 1500ml with 50% floods 3d in room temperature, filter, the filtrate decompression concentration and recovery solvent of getting three Plants obtains extracting medicinal extract, then mix these three kinds and extract medicinal extract (wherein, the blue medicinal extract that extracts of Song occupies effect composition total amount 25%, garlic 35%, silk tree 34.95%), 5.0% Imidacloprid of rear adding active ingredient total amount and 0.05% antitoxin rich, and then adding weight accounts for the tween of whole antivirus compound formulation 10%, stir according to common process, it is mixed, make the compound preparation of the anti-gourd vegetables viroses of plant.And then dilute with water, being mixed with active ingredient concentration is the compound preparation of the anti-gourd vegetables viroses of plant of 5wt%.This compound preparation is carried out field control effectiveness test to Summer squash virus.At field Summer squash virus initial stage of origination, with the medicament for preparing, foliar spray, statistical results after 7 days, preventive effect reaches 72.9%.
Embodiment 4
The anti-gourd vegetables viroses of plant compound preparation that active ingredient 40% and conventional auxiliary material 60% form
Take by weighing respectively the blue stem of Song, leaf, flower or its mixture and garlic are pruned or the rear discarded stem of results, leaf, the discarded branch that silk tree is pruned or naturally comes off, leaf, each 200g of powder (20 order particle) of flower, ethanolic solution 2000ml with 60% floods 3d in room temperature, filter, the filtrate decompression concentration and recovery solvent of getting three Plants obtains extracting medicinal extract, then mix these three kinds and extract medicinal extract (wherein, the blue medicinal extract that extracts of Song occupies effect composition total amount 5%, garlic 50%, silk tree 34.95%), 10.0% Imidacloprid of rear adding active ingredient total amount and 0.05% antitoxin rich, and then adding weight accounts for the tween of whole antivirus compound formulation 60%, stir according to common process, it is mixed, make the compound preparation of the anti-gourd vegetables viroses of plant.And then dilute with water, being mixed with active ingredient concentration is the compound preparation of the anti-gourd vegetables viroses of plant of 5wt%.This compound preparation is carried out field control effectiveness test to fragrant sponge gourd virus disease.The fragrant sponge gourd virus disease initial stage of origination in the field, with the medicament for preparing, foliar spray, statistical results after 7 days, preventive effect reaches 60.3%.
Embodiment 5
The anti-gourd vegetables viroses of plant compound preparation that active ingredient 50% and conventional auxiliary material 50% form
Take by weighing respectively the blue stem of Song, leaf, flower or its mixture and garlic are pruned or the rear discarded stem of results, leaf, the discarded branch that silk tree is pruned or naturally comes off, leaf, each 300g of powder (30 order particle) of flower, ethanolic solution 2000ml with 90% floods 7d in room temperature, filter, the filtrate decompression concentration and recovery solvent of getting three Plants obtains extracting medicinal extract, then mix these three kinds and extract medicinal extract (wherein, the blue medicinal extract that extracts of Song occupies effect composition total amount 50%, garlic 5%, silk tree 39%), the Imidacloprid of rear adding active ingredient total amount 5.0% and 1.0% antitoxin rich, and then adding weight accounts for the tween of whole antivirus compound formulation 50%, stir according to common process, it is mixed, make the compound preparation of the anti-gourd vegetables viroses of plant.And then dilute with water, being mixed with active ingredient concentration is the compound preparation of the anti-gourd vegetables viroses of plant of 5wt%.This compound preparation is carried out field control effectiveness test to squash virus.At field squash virus initial stage of origination, with the medicament for preparing, foliar spray, statistical results after 7 days, preventive effect reaches 69.8%.
Embodiment 6
The anti-gourd vegetables viroses of plant compound preparation that active ingredient 60% and conventional auxiliary material 40% form
Take by weighing respectively the blue stem of Song, leaf, flower or its mixture and garlic are pruned or the rear discarded stem of results, leaf, the discarded branch that silk tree is pruned or naturally comes off, leaf, each 300g of powder (30 order particle) of flower, ethanolic solution 2000ml with 10% floods 3d in room temperature, filter, the filtrate decompression concentration and recovery solvent of getting three Plants obtains extracting medicinal extract, then mix these three kinds and extract medicinal extract (wherein, the blue medicinal extract that extracts of Song occupies effect composition total amount 25%, garlic 50%, silk tree 19%), the Imidacloprid of rear adding active ingredient total amount 3.0% and 3.0% antitoxin rich, and then adding weight accounts for the tween of whole antivirus compound formulation 40%, stir according to common process, it is mixed, make the compound preparation of the anti-gourd vegetables viroses of plant.And then dilute with water, being mixed with active ingredient concentration is the compound preparation of the anti-gourd vegetables viroses of plant of 0.5wt%.This compound preparation is carried out field control effectiveness test to Summer squash virus.At field Summer squash virus initial stage of origination, with the medicament for preparing, foliar spray, statistical results after 7 days, preventive effect reaches 50.4%.
More than specific embodiments of the invention are described.It will be appreciated that the present invention is not limited to above-mentioned particular implementation, those skilled in the art can make various distortion or modification within the scope of the claims, and this does not affect flesh and blood of the present invention.
Claims (10)
1. the compound preparation of anti-gourd vegetables viroses of plant, it is characterized in that described preparation is active ingredient by plant extracts and chemical pesticide, described active ingredient weight content is 40%~90%, and all the other are to allow in the agricultural chemicals to use and acceptable conventional auxiliary material composition;
The weight percentage of the component of described active ingredient and shared active ingredient total amount is: the blue extract 5~50% of Song, garlic P.E 5~50%, albizzia julibrissin extract 2~40%, Imidacloprid 0.01~10%, antitoxin rich 0.01~5%; Wherein:
Described Song blue (Isatis tinctoria L.) takes from the blue stem of Song, leaf, flower or its mixture; The blue extract of described Song is to clay into power after the blue oven dry of Song, again through lixiviate, to filter, and reduced pressure concentration obtains;
Described garlic (Allium sativum L.) is taken from stem, the leaf of discarding after garlic is pruned or gathers in the crops; Described garlic P.E is with claying into power after the garlic oven dry, again through lixiviate, filters, and reduced pressure concentration obtains;
Described silk tree (Albizia julibrissin D.) is taken from discarded branch, leaf or the root that silk tree is pruned or naturally comes off; Described albizzia julibrissin extract is with claying into power after the silk tree oven dry, again through lixiviate, filters, and reduced pressure concentration obtains.
2. the compound preparation of the anti-gourd vegetables viroses of plant according to claim 1 is characterized in that, described conventional auxiliary material refers to promote extract dissolving or solubilizer, emulsifier and/or the suspending agent, synergist, the bleeding agent that suspend.
3. the preparation method of the compound preparation of the compound preparation of anti-gourd vegetables viroses of plant claimed in claim 1, it is characterized in that, described method is by respectively with the blue stem of Song, leaf, flower or its mixture and garlic are pruned or the rear discarded stem of results, leaf, the discarded branch that silk tree is pruned or naturally comes off, leaf, flower or its mixture, clay into power after the oven dry, with ethanolic solution as extraction solvent, lixiviate, filter, filtrate adopts the method for reduced pressure concentration to obtain respectively medicinal extract, and then add Imidacloprid and antitoxin rich, and add at last conventional auxiliary material, make the compound preparation of the described anti-gourd vegetables viroses of plant.
4. the preparation method of the compound preparation of the anti-gourd vegetables viroses of plant according to claim 3 is characterized in that, described oven dry refers to: 30-50 ℃ of lower oven dry.
5. the preparation method of the compound preparation of the anti-gourd vegetables viroses of plant according to claim 3 is characterized in that, described powder refers to: 20-40 purpose granulated powders.
6. the preparation method of the compound preparation of the anti-gourd vegetables viroses of plant according to claim 3 is characterized in that, described ethanol refers to ethanolic solution or the absolute ethyl alcohol of 10%-95%v/v, and its consumption is 10-20 times of powder quality.
7. the preparation method of the compound preparation of the anti-gourd vegetables viroses of plant according to claim 3 is characterized in that, described lixiviate, and its time is 1-7 days.
8. the preparation method of the compound preparation of the anti-gourd vegetables viroses of plant according to claim 3, it is characterized in that, described reduced pressure concentration refers to: the crude extract that obtains after the lixiviate is vacuumized decompression to-0.1 atmospheric pressure and places 30-80 ℃ of lower water-bath, make crude extract simmer down to medicinal extract.
9. the application of the compound preparation of anti-gourd vegetables viroses of plant claimed in claim 1 is characterized in that, under stirring at normal temperature water is added in the described compound preparation, its active ingredient that is diluted to preparation is accounted for spray after the concentration of 0.5-5wt% to use.
10. the application of the compound preparation of the anti-gourd vegetables viroses of plant according to claim 9 is characterized in that, it is sick that described compound preparation is applied in control gourd vegetables Plant virus.
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CN106333009A (en) * | 2016-10-11 | 2017-01-18 | 福建八马茶业有限公司 | Method for preparing isatis tinctoria honey tea |
CN108041095A (en) * | 2017-12-08 | 2018-05-18 | 柳州雅瑞科技有限公司 | A kind of botanical fungicide for preventing edible lily virosis and preparation method thereof |
CN110477054A (en) * | 2018-08-28 | 2019-11-22 | 郑州海绿王绿色科技有限公司 | Plant extracts composite growth regulator containing guaiacol |
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