CN103030618A - Naringenin extraction technology - Google Patents
Naringenin extraction technology Download PDFInfo
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- CN103030618A CN103030618A CN2011102905508A CN201110290550A CN103030618A CN 103030618 A CN103030618 A CN 103030618A CN 2011102905508 A CN2011102905508 A CN 2011102905508A CN 201110290550 A CN201110290550 A CN 201110290550A CN 103030618 A CN103030618 A CN 103030618A
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- extraction
- naringenin
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- peach leaf
- extraction technology
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P20/00—Technologies relating to chemical industry
- Y02P20/50—Improvements relating to the production of bulk chemicals
- Y02P20/54—Improvements relating to the production of bulk chemicals using solvents, e.g. supercritical solvents or ionic liquids
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Abstract
The invention relates to a flavonoid substance extraction technology, and specifically relates to a naringenin extraction technology. The naringenin extraction technology has the advantages of short time consumption, small consumed energy, and high extraction rate. The naringenin extraction technology utilizes a supercritical carbon dioxide extraction technique, treats peach leaves as a raw material to extract naringenin, and comprises the steps of peach leaf drying, crushing, sieving, extracting, concentrating, and drying to obtain a finished product.
Description
Technical field:
The present invention relates to a kind of extraction process of Flavonoid substances, more particularly, relate to the extraction process of naringenin.
Background technology:
Naringenin belongs to Flavonoid substances, and it is the glucoside unit of naringin.Naringenin is the white crystals fine powder, belongs to flavanone kind composition, white, needle-shaped crystals, and 260 ℃ of fusing points, naringenin is dissolved in ethanol, and ether and benzene are water-soluble hardly, and molecular formula is C
15H
12O
5, molecular weight is 272.25, structural formula is as shown below.
Naringenin
That naringenin has is antibiotic, anti-inflammatory, anticancer, spasmolysis and choleretic effect:
1. antibiotic: that streptococcus aureus, large intestine, dysentery and Corynebacterium diphtheriae are had stronger anti-microbial effect.Naringenin also has effect to fungi, and 1000ppm is sprayed on and can reduces rice blast fungus infection 40-90% on the rice, and people and domestic animal are not had toxicity.
2. anti-inflammatory: rat abdominal cavity is injected 20mg/kg every day, obviously suppresses to implant the caused inflammatory process of wool ball.
3. anticancer: that large murine leukemia L1210 and sarcoma are all had activity.
4. spasmolysis and cholagogic: pretend the user for having in the flavonoid compound.Naringenin also has the effect of stronger increase laboratory animal choleresis.
Present stage, also less to the exploitation of naringenin, the extraction process of naringenin also rests on power consumption length large, consuming time, primary stage that extraction yield is low.
Summary of the invention:
The present invention is exactly for the problems referred to above, and the extraction process of a kind of weak point consuming time, power consumption is little, extraction yield is high naringenin is provided.
In order to realize above-mentioned purpose of the present invention, the present invention adopts following technical scheme, and the present invention utilizes supercritical carbon dioxide extraction method, carries out the extraction of naringenin take peach leaf as raw material, and processing step is:
Peach leaf dry, pulverize, sieve
The peach leaf that the present invention selects to dry extracts;
2. extraction
Add solid-liquid ratio in the peach leaf powder and be 1: 2～1: 5 ethanol, join in the extraction kettle after stirring, pressure is made as 25～35MPa in the still, at 40～60 ℃ of lower extraction 2～6h;
3. concentrated, dry, finished product.
Beneficial effect of the present invention:
The present invention's weak point simple to operate, consuming time, the power consumption little;
2. extraction yield of the present invention is 2.18%.
Embodiment:
Processing step of the present invention is:
Peach leaf dry, pulverize, sieve
The peach leaf that the present invention selects to dry extracts;
2. extraction
Add solid-liquid ratio in the peach leaf powder and be 1: 2～1: 5 ethanol, join in the extraction kettle after stirring, pressure is made as 25～35MPa in the still, at 40～60 ℃ of lower extraction 2～6h;
3. concentrated, dry, finished product.
The present invention selects solid-liquid ratio, extracting pressure, extraction temperature, extraction time to affect the most the factor of naringenin extraction effect, can see Table 1, table 2.
Table 1 four level of factor tables
Table 2 four factors are on the impact of naringenin extraction effect
| Sequence number | A | B | C | D | Naringenin yield/% |
| 1 | 1 | 1 | 1 | 1 | 0.24 |
| 2 | 1 | 2 | 2 | 2 | 0.45 |
| 3 | 1 | 3 | 3 | 3 | 0.67 |
| 4 | 2 | 1 | 2 | 3 | 0.81 |
| 5 | 2 | 2 | 3 | 1 | 1.91 |
| 6 | 2 | 3 | 1 | 2 | 1.44 |
| 7 | 3 | 1 | 3 | 2 | 1.74 |
| 8 | 3 | 2 | 1 | 3 | 1.69 |
| 9 | 3 | 3 | 2 | 1 | 1.67 |
As a kind of preferred version, processing step of the present invention is: solid-liquid ratio is 1: 2.0; Extracting pressure is 30MPa, and extraction temperature is 60 ℃, and extraction time is 6h.
As can be seen from Table 2, extraction temperature has the greatest impact to the result.Temperature raises, and the mass transfer velocity of fluid increases, and is beneficial to mass transfer; Temperature raises, and the volatility that is extracted material increases, and is beneficial to the stripping of composition.When temperature was increased to 50 ℃ by 40 ℃, the naringenin yield had a more substantial increase; Be increased to 60 ℃ and work as temperature by 50 ℃, the amplitude that yield increases is just large less than the former.This is because when the increase of temperature, CO
2Fluid density has also reduced thereupon, and this has produced a negative impact to extraction.The amplitude that temperature increases is larger, and the effect of this negative impact is also more obvious.
Extraction time is also larger on the impact of extraction results.When extraction time reached 6h, it is maximum that extraction results reaches.Extracting pressure is on the impact of the extraction results impact inferior to extraction time.The increase of pressure, supercritical CO
2The density of fluid increases, and is conducive to the stripping of composition; Pressure increases, supercritical CO
2The spread coefficient of fluid also reduces thereupon, is unfavorable for the stripping of composition.When pressure was increased to 30MPa by 25MPa, extraction results obviously increased; But when pressure was increased to 35MPa by 30MPa, extraction results had but descended, and this is because the negative impact that this moment, spread coefficient reduced has play a part larger.
Claims (3)
1. the extraction process of naringenin is characterized in that, the present invention utilizes supercritical carbon dioxide extraction method, carries out the extraction of naringenin take peach leaf as raw material, and processing step is:
(1) peach leaf dry, pulverize, sieve
The peach leaf that the present invention selects to dry extracts;
(2) extraction
Add solid-liquid ratio in the peach leaf powder and be 1: 2～1: 5 ethanol, join in the extraction kettle after stirring, pressure is made as 25～35MPa in the still, at 40～60 ℃ of lower extraction 2～6h;
(3) concentrated, dry, finished product.
2. the extraction process of naringenin according to claim 1 is characterized in that, processing step of the present invention is: the solid-liquid ratio of peach leaf and ethanol is 1: 2.0.
3. the extraction process of naringenin according to claim 1 is characterized in that, processing step of the present invention is: extracting pressure is 30MPa, and extraction temperature is 60 ℃, and extraction time is 6h.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011102905508A CN103030618A (en) | 2011-09-29 | 2011-09-29 | Naringenin extraction technology |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011102905508A CN103030618A (en) | 2011-09-29 | 2011-09-29 | Naringenin extraction technology |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN103030618A true CN103030618A (en) | 2013-04-10 |
Family
ID=48018065
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2011102905508A Pending CN103030618A (en) | 2011-09-29 | 2011-09-29 | Naringenin extraction technology |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103030618A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103896898A (en) * | 2014-03-07 | 2014-07-02 | 中国计量科学研究院 | Naringenin standard substance as well as preparation and application thereof |
| CN104277024A (en) * | 2014-09-29 | 2015-01-14 | 桂林莱茵生物科技股份有限公司 | Method for extracting naringenin from grapefruits |
| CN105713757A (en) * | 2016-01-28 | 2016-06-29 | 佛山市聚成生化技术研发有限公司 | Grape wine for female and containing peach leaf naringenin |
-
2011
- 2011-09-29 CN CN2011102905508A patent/CN103030618A/en active Pending
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103896898A (en) * | 2014-03-07 | 2014-07-02 | 中国计量科学研究院 | Naringenin standard substance as well as preparation and application thereof |
| CN103896898B (en) * | 2014-03-07 | 2016-04-20 | 中国计量科学研究院 | A kind of naringenin reference material and Synthesis and applications thereof |
| CN104277024A (en) * | 2014-09-29 | 2015-01-14 | 桂林莱茵生物科技股份有限公司 | Method for extracting naringenin from grapefruits |
| CN104277024B (en) * | 2014-09-29 | 2016-02-10 | 桂林莱茵生物科技股份有限公司 | A kind of method extracting naringenin from shaddock |
| CN105713757A (en) * | 2016-01-28 | 2016-06-29 | 佛山市聚成生化技术研发有限公司 | Grape wine for female and containing peach leaf naringenin |
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Application publication date: 20130410 |