CN104478841A - Method for extracting flavone from vine tea - Google Patents
Method for extracting flavone from vine tea Download PDFInfo
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- CN104478841A CN104478841A CN201410691068.9A CN201410691068A CN104478841A CN 104478841 A CN104478841 A CN 104478841A CN 201410691068 A CN201410691068 A CN 201410691068A CN 104478841 A CN104478841 A CN 104478841A
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- Prior art keywords
- vine tea
- extract
- ultrasonic
- microwave
- powder
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07D—HETEROCYCLIC COMPOUNDS
- C07D311/00—Heterocyclic compounds containing six-membered rings having one oxygen atom as the only hetero atom, condensed with other rings
- C07D311/02—Heterocyclic compounds containing six-membered rings having one oxygen atom as the only hetero atom, condensed with other rings ortho- or peri-condensed with carbocyclic rings or ring systems
- C07D311/04—Benzo[b]pyrans, not hydrogenated in the carbocyclic ring
- C07D311/22—Benzo[b]pyrans, not hydrogenated in the carbocyclic ring with oxygen or sulfur atoms directly attached in position 4
- C07D311/26—Benzo[b]pyrans, not hydrogenated in the carbocyclic ring with oxygen or sulfur atoms directly attached in position 4 with aromatic rings attached in position 2 or 3
- C07D311/28—Benzo[b]pyrans, not hydrogenated in the carbocyclic ring with oxygen or sulfur atoms directly attached in position 4 with aromatic rings attached in position 2 or 3 with aromatic rings attached in position 2 only
- C07D311/30—Benzo[b]pyrans, not hydrogenated in the carbocyclic ring with oxygen or sulfur atoms directly attached in position 4 with aromatic rings attached in position 2 or 3 with aromatic rings attached in position 2 only not hydrogenated in the hetero ring, e.g. flavones
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07D—HETEROCYCLIC COMPOUNDS
- C07D311/00—Heterocyclic compounds containing six-membered rings having one oxygen atom as the only hetero atom, condensed with other rings
- C07D311/02—Heterocyclic compounds containing six-membered rings having one oxygen atom as the only hetero atom, condensed with other rings ortho- or peri-condensed with carbocyclic rings or ring systems
- C07D311/04—Benzo[b]pyrans, not hydrogenated in the carbocyclic ring
- C07D311/22—Benzo[b]pyrans, not hydrogenated in the carbocyclic ring with oxygen or sulfur atoms directly attached in position 4
- C07D311/26—Benzo[b]pyrans, not hydrogenated in the carbocyclic ring with oxygen or sulfur atoms directly attached in position 4 with aromatic rings attached in position 2 or 3
- C07D311/40—Separation, e.g. from natural material; Purification
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07G—COMPOUNDS OF UNKNOWN CONSTITUTION
- C07G99/00—Subject matter not provided for in other groups of this subclass
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- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Extraction Or Liquid Replacement (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Abstract
The invention discloses a method for extracting flavone from vine tea. The method comprises the steps of pretreating, extracting, filtering, centrifuging and drying. The method has high extraction efficiency, is low in cost and can realize industrial production easily.
Description
Technical field
The invention belongs to technical field of agricultural product process, particularly relate to a kind of method extracting flavones from vine tea.
Background technology
Vine tea, formal name used at school is Ampelopsis grossedentata [Ampelopsis grossedentata (Hand-Mazz) W.T.Wang], for the wild wooden defoliation liana of one of Vitaceae (Vitaceae Michx) Ampelopsis (Ampelopsis), belong to typical class tea plant, be commonly called as the sweet tea in mountain, white tea, manna tea, white hair monkey, white tea etc., be mainly distributed in the ground such as China Hunan, Hubei, Yunnan, Guizhou, Guangdong, Guangxi, Fujian.The former plant-growth of vine tea is between height above sea level 400-1300m, and concentrate or scatteredly mix in woods on hillside, wild reserves are large, distribution and growth rhythm more clear, be the wild plant resource that can directly utilize.
Vine tea is not only a kind of green drink of pure natural, wonderful work in the tea that a kind of drug effect health properties is very strong especially, it contains the trace element such as iron, zinc, calcium, magnesium, potassium, sodium of the protein of needed by human body, food fibre, fat, carotene, VITAMIN and needed by human body, Sheng, pharyngolaryngitis, hypertension etc. are crossed to prevention irascibility and has effect unique, also there is clearing heat and detoxicating, the beautifying and antisenility effect of waiting for a long time, the people not high concerning sleep quality, especially can improving water flood quality.
Main component in vine tea is flavonoid compound.In recent years, more existing scholars are studied the method extracting flavones from vine tea, and they take organic solvent as extraction agent usually, and take the method for water-bath lixiviate, and this class methods efficiency is low, and cost is high, and organic solvent easily causes environmental pollution.
Summary of the invention
In order to overcome the deficiencies in the prior art, the object of this invention is to provide a kind of method extracting flavones from vine tea, the problem that solve improves the extraction efficiency of flavones, reduces extraction cost.Technical scheme provided by the present invention is:
From vine tea, extract a method for flavones, it is characterized in that, the method comprises the following steps:
(1) pre-treatment: dried vine tea is crushed to 60-200 order, obtains vine tea powder.
(2) extract: be that 1:5-1:40 adds vine tea powder and water respectively according to solid-to-liquid ratio, add buffered soln and adjust pH7.0-7.5, soak, be then placed in microwave-ultrasonic in combination and extract 3-5 time, obtain vine tea crude extract;
(3) filter: by gained vine tea crude extract after filtration, collect filter residue;
(4) dry: by spray-dried for the filter residue collected in step (3), namely to obtain flavone powder.
Wherein, in step (1), dried sweet tea powder is broken to 120 orders.
Wherein, the buffered soln described in step (2) is acetic acid or sodium carbonate buffer.
Wherein, the time of step (2) described immersion is 30-60min.
Wherein, during the described microwave of step (2)-ultrasonic in combination extracts, microwave power is 80-280W, and ultrasonic power is 100-250W, and extraction time is 1-2h.
beneficial effect of the present invention
1, the microwave-ultrasonic in combination extraction method of the present invention's employing, effectively can shorten extraction time and improve extraction efficiency.
2, the present invention adopts water as extraction solution, adopts acetic acid and sodium-acetate as buffered soln, greatly reduces production cost, and can improve the extraction yield of flavones.
Embodiment
To be easier to understand the present invention with reference to the following example, the embodiment provided does not limit the scope of the invention.
embodiment 1
Take the vine tea powder that 1g crosses 60 mesh sieves, water is added with solid-to-liquid ratio 1:5, sodium carbonate solution regulates pH to be 7.0, after cold soaking 30min, and 43 DEG C, in adopting microwave-ultrasonic in combination to extract, microwave power is 80W, ultrasonic power is 250W, and extraction time is 2h, repeats 3 times, filter residue is collected, the spray-dried powder obtaining flavones after suction filtration.After measured, the flavones that the method obtains is 0.32g, and extraction rate reached is to 32%.
embodiment 2
Take the vine tea powder that 1g crosses 200 mesh sieves, water is added with solid-to-liquid ratio 1:40, sodium carbonate solution regulates pH to be 7.5, after cold soaking 60min, and 43 DEG C, in adopting microwave-ultrasonic in combination to extract, microwave power is 280W, ultrasonic power is 100W, and extraction time is 1h, repeats 3 times, filter residue is collected, the spray-dried powder obtaining flavones after suction filtration.After measured, the flavones that the method obtains is 0.35g, and extraction rate reached is to 35%.
embodiment 3
Take the vine tea powder that 1g crosses 150 mesh sieves, water is added with solid-to-liquid ratio 1:20, acetum regulates pH to be 7.3, after cold soaking 40min, and 43 DEG C, in adopting microwave-ultrasonic in combination to extract, microwave power is 200W, ultrasonic power is 150W, and extraction time is 1.5h, repeats 3 times, filter residue is collected, the spray-dried powder obtaining flavones after suction filtration.After measured, the flavones that the method obtains is 0.38g, and extraction rate reached is to 38%.
The aforementioned description to concrete exemplary of the present invention is to illustrate and the object of illustration.These descriptions not want the present invention to be defined as disclosed precise forms, and obviously, according to above-mentioned instruction, can much change and change.The object selected exemplary embodiment and describe is to explain certain principles of the present invention and practical application thereof, thus those skilled in the art can be realized and utilize various different exemplary of the present invention and various different selection and change.Scope of the present invention is intended to limited by claims and equivalents thereof.
Claims (5)
1. from vine tea, extract a method for flavones, it is characterized in that, the method comprises the following steps:
(1) pre-treatment: dried vine tea is crushed to 60-200 order, obtains vine tea powder.
(2) extract: be that 1:5-1:40 adds vine tea powder and water respectively according to solid-to-liquid ratio, add buffered soln and adjust pH7.0-7.5, soak, be then placed in microwave-ultrasonic in combination and extract 3-5 time, obtain vine tea crude extract;
(3) filter: by gained vine tea crude extract after filtration, collect filter residue;
(4) dry: by spray-dried for the filter residue collected in step (3), namely to obtain flavone powder.
2. method according to claim 1, is characterized in that: dried sweet tea powder is broken to 120 orders in (1) by step.
3. method according to claim 1, is characterized in that: the buffered soln described in step (2) is acetic acid or sodium carbonate buffer.
4. method according to claim 1, is characterized in that: the time of step (2) described immersion is 30-60min.
5. method according to claim 1, is characterized in that: during the described microwave of step (2)-ultrasonic in combination extracts, microwave power is 80-280W, and ultrasonic power is 100-250W, and extraction time is 1-2h.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410691068.9A CN104478841A (en) | 2014-11-25 | 2014-11-25 | Method for extracting flavone from vine tea |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410691068.9A CN104478841A (en) | 2014-11-25 | 2014-11-25 | Method for extracting flavone from vine tea |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN104478841A true CN104478841A (en) | 2015-04-01 |
Family
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410691068.9A Pending CN104478841A (en) | 2014-11-25 | 2014-11-25 | Method for extracting flavone from vine tea |
Country Status (1)
| Country | Link |
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| CN (1) | CN104478841A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106581319A (en) * | 2016-12-28 | 2017-04-26 | 贵阳中医学院 | Ultrasonic extraction technology of ampelopsis grossedentata total flavonoids |
| CN112390773A (en) * | 2020-12-02 | 2021-02-23 | 河南智盛优品生物科技有限公司 | Extraction process of vine tea flavone |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101810735A (en) * | 2010-04-16 | 2010-08-25 | 安徽省瑞森生物科技有限责任公司 | Method for extracting total flavone from ampelopsis grossedentata |
| CN102106931A (en) * | 2010-12-30 | 2011-06-29 | 张家界茅岩莓有限公司 | Method for producing diverse extracts of berry tea |
| CN102796069A (en) * | 2012-07-04 | 2012-11-28 | 贵州省生物研究所 | Method for extracting dihydromyricetin and Ampelopsis grossedentata polysaccharide from Ampelopsis grossedentata |
| CN103467427A (en) * | 2013-09-02 | 2013-12-25 | 江西省科学院应用化学研究所 | Method for comprehensively extracting dihydromyricetin and polysaccharide in vine tea at low temperature |
-
2014
- 2014-11-25 CN CN201410691068.9A patent/CN104478841A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101810735A (en) * | 2010-04-16 | 2010-08-25 | 安徽省瑞森生物科技有限责任公司 | Method for extracting total flavone from ampelopsis grossedentata |
| CN102106931A (en) * | 2010-12-30 | 2011-06-29 | 张家界茅岩莓有限公司 | Method for producing diverse extracts of berry tea |
| CN102796069A (en) * | 2012-07-04 | 2012-11-28 | 贵州省生物研究所 | Method for extracting dihydromyricetin and Ampelopsis grossedentata polysaccharide from Ampelopsis grossedentata |
| CN103467427A (en) * | 2013-09-02 | 2013-12-25 | 江西省科学院应用化学研究所 | Method for comprehensively extracting dihydromyricetin and polysaccharide in vine tea at low temperature |
Non-Patent Citations (1)
| Title |
|---|
| 曾云想 等.: "藤茶总黄酮提取方法的优选", 《山地农业生物学报》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106581319A (en) * | 2016-12-28 | 2017-04-26 | 贵阳中医学院 | Ultrasonic extraction technology of ampelopsis grossedentata total flavonoids |
| CN112390773A (en) * | 2020-12-02 | 2021-02-23 | 河南智盛优品生物科技有限公司 | Extraction process of vine tea flavone |
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Application publication date: 20150401 |