CN102225923A - Method for preparing dihydromyricetin from Ampelopsis grossedentata - Google Patents
Method for preparing dihydromyricetin from Ampelopsis grossedentata Download PDFInfo
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- CN102225923A CN102225923A CN2011100933467A CN201110093346A CN102225923A CN 102225923 A CN102225923 A CN 102225923A CN 2011100933467 A CN2011100933467 A CN 2011100933467A CN 201110093346 A CN201110093346 A CN 201110093346A CN 102225923 A CN102225923 A CN 102225923A
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Abstract
A method for preparing dihydromyricetin from Ampelopsis grossedentata relates to the bio-medicine field, and comprises the following steps of: carefully choosing finished products with many white crystals on the surface; adding 30-90% concentration of an ethanol aqueous solution 5-20 times the weight of the Ampelopsis grossedentata for each extraction treatment; extracting for 25 times by heating reflux with 1-3 hours for each extraction; collecting an extract, recovering ethanol by decompressing method until there is no alcohol smell; letting cool until yellow primary crystals precipitate, carrying out pumping filtration to filter out yellow primary crystals; adding boiling water until the yellow primary crystals are completely dissolved; letting cool until new light yellow crystals precipitate; carrying out pumping filtration again to filter out light yellow crystals; dissolving by 70% of ethanol reflux, decolouring by the addition of 3% of active carbon, followed by pumping filtration; condensing the filtrate by decompressing until there is no alcohol; letting cool until white crystals precipitate; finally crystallizing repeatedly by the use of methanol until white crystals precipitate, followed by vacuum drying to obtain a white powder. The method provided by the invention can be adopted to extract 98% of dihydromyricetin from Ampelopsis grossedentata, extract high-content effective compositions with good curative effect and stable quality.
Description
Technical field:
The present invention relates to biomedicine field, be specifically related to the preparation method of dibydro myricetrin in the vine tea.
Background technology:
Vine tea is commonly called as Maoyanmei tea, Dragon Boat Festival tea, rattan mother-in-law tea (claiming the sweet tea in mountain, dragon palpus tea again), is the young stem and leaf of the Vitaceae Ampelopsis Ampelopsis grossedentata W.T.wang of section.Pluck browse summer, put that rapid iron picks up immediately in the boiling water once, drain away the water, spread the ventilation and dry up, during existing asterism bloom, can dry collection to the surface.Mainly be distributed in provinces and regions such as Guangdong, Guangxi, Yunnan, Guizhou, Hunan, Jiangxi, Fujian.The sweet length of mildly bitter flavor promotes the production of body fluid to quench thirst, and its flavor is sweet cool in nature; Have clearing heat and detoxicating, antisepsis and anti-inflammation, dispel rheumatism, effect such as strengthening the bones and muscles, hypotensive, reducing blood-fat, liver protecting.Be usually used in the control of diseases such as essential hypertension, cold, fever, cardiovascular and cerebrovascular diseases, dermatitis, eczema.Modern study shows that mainly effectively becoming in the vine tea is flavonoid compound, and be the highest with the content of dibydro myricetrin.Many biological activitys such as dibydro myricetrin has anti-inflammatory, cough-relieving, eliminates the phlegm, analgesia, antibacterial, lipopenicillinase, antitumor, hypertension, liver protecting, great exploitation potential for its.
Summary of the invention:
The preparation method who the purpose of this invention is to provide dibydro myricetrin in the vine tea, it can extract content in the vine tea is 98% dibydro myricetrin, extracts the effective component content height, good effect, steady quality; Can be hypotensive, blood fat reducing and cholesterol, microcirculation improvement, the enhance immunity ability, anti-oxidant, anti-ageing, antitumor, antisepsis and anti-inflammation, anti-arrhythmia, resist myocardial ischemia, prevention alcoholic liver, fatty liver, inhibition liver cell worsen, reduce the sickness rate of liver cancer, liver protecting, Dealcoholic sobering-up and SOD activity improving suppress the formation of external platelet set and thrombus in vivo.
In order to solve the existing problem of background technology, the present invention is by the following technical solutions: its preparation method is: the more finished product of selected surperficial white crystals, and it is that 5-20 concentration doubly is the 30-90% aqueous ethanolic solution that each extraction adding accounts for vine tea weight, heating and refluxing extraction 2-5 time, the each extraction 1-3 hour, collect extracting solution, decompression recycling ethanol is put to be chilled to and is separated out yellow primary crystal to there not being the alcohol flavor, suction filtration, leach yellow primary crystal, add boiling water paper and dissolve fully, put to be chilled to and separate out new light yellow crystallization, suction filtration again, leach light yellow crystallization,, add about 3% activated carbon decolorizing with the dissolving of 70% alcohol reflux, suction filtration, filtrate decompression being concentrated into does not have alcohol again, puts to be chilled to and separates out off-white color crystallization, suction filtration, leach the off-white color crystallization, at last with the methyl alcohol periodic crystallisation to separating out white crystals, after the vacuum-drying white powder, be dibydro myricetrin.
The present invention has following beneficial effect: can extract content in the vine tea and be 98% dibydro myricetrin, extract the effective component content height, good effect, steady quality; Can be hypotensive, blood fat reducing and cholesterol, microcirculation improvement, the enhance immunity ability, anti-oxidant, anti-sorrow is old, antitumor, antisepsis and anti-inflammation, anti-arrhythmia, resist myocardial ischemia, prevention alcoholic liver, fatty liver, inhibition liver cell worsen, reduce the sickness rate of liver cancer, liver protecting, Dealcoholic sobering-up and SOD activity improving suppress the formation of external platelet set and thrombus in vivo.
Embodiment:
This embodiment is by the following technical solutions: its preparation method is: the more finished product of selected surperficial white crystals, it is that 5-20 concentration doubly is the 30-90% aqueous ethanolic solution that each extraction adding accounts for vine tea weight, heating and refluxing extraction 2-5 time was extracted 1-3 hour at every turn, collected extracting solution, decompression recycling ethanol is to there not being the alcohol flavor, put to be chilled to and separate out yellow primary crystal, suction filtration leaches yellow primary crystal, adding boiling water paper dissolves fully, put to be chilled to and separate out new light yellow crystallization, suction filtration leaches light yellow crystallization again, dissolve with 70% alcohol reflux, add about 3% activated carbon decolorizing, suction filtration, filtrate decompression is concentrated into does not have alcohol again, put to be chilled to and separate out the off-white color crystallization, suction filtration leaches the off-white color crystallization, at last with the methyl alcohol periodic crystallisation to separating out white crystals, get white powder after the vacuum-drying, be dibydro myricetrin.
It is 98% dibydro myricetrin that this embodiment can be extracted content in the vine tea, extracts the effective component content height, good effect, steady quality; Can be hypotensive, blood fat reducing and cholesterol, microcirculation improvement, the enhance immunity ability, anti-oxidant, anti-ageing, antitumor, antisepsis and anti-inflammation, anti-arrhythmia, resist myocardial ischemia, prevention alcoholic liver, fatty liver, inhibition liver cell worsen, reduce the sickness rate of liver cancer, liver protecting, Dealcoholic sobering-up and SOD activity improving suppress the formation of external platelet set and thrombus in vivo.
Claims (1)
1. the preparation method of dibydro myricetrin in the vine tea, the preparation method who it is characterized in that it is: the more finished product of selected surperficial white crystals, and it is that 5-20 concentration doubly is the 30-90% aqueous ethanolic solution that each extraction adding accounts for vine tea weight, heating and refluxing extraction 2-5 time, the each extraction 1-3 hour, collect extracting solution, decompression recycling ethanol is put to be chilled to and is separated out yellow primary crystal to there not being the alcohol flavor, suction filtration, leach yellow primary crystal, add boiling water paper and dissolve fully, put to be chilled to and separate out new light yellow crystallization, suction filtration again, leach light yellow crystallization,, add about 3% activated carbon decolorizing with the dissolving of 70% alcohol reflux, suction filtration, filtrate decompression being concentrated into does not have alcohol again, puts to be chilled to and separates out off-white color crystallization, suction filtration, leach the off-white color crystallization, at last with the methyl alcohol periodic crystallisation to separating out white crystals, after the vacuum-drying white powder, be dibydro myricetrin.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN2011100933467A CN102225923A (en) | 2011-04-14 | 2011-04-14 | Method for preparing dihydromyricetin from Ampelopsis grossedentata |
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| Application Number | Priority Date | Filing Date | Title |
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| CN2011100933467A CN102225923A (en) | 2011-04-14 | 2011-04-14 | Method for preparing dihydromyricetin from Ampelopsis grossedentata |
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| CN102225923A true CN102225923A (en) | 2011-10-26 |
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| CN2011100933467A Pending CN102225923A (en) | 2011-04-14 | 2011-04-14 | Method for preparing dihydromyricetin from Ampelopsis grossedentata |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2014012075A3 (en) * | 2012-07-13 | 2014-03-06 | Phyto Tech Corp. | Treating hair loss and delaying aging of skin |
| CN103772338A (en) * | 2014-01-10 | 2014-05-07 | 吉首大学 | Vacuum pulse type method of preparing dihydromyricetin |
| CN106967030A (en) * | 2017-05-23 | 2017-07-21 | 西安天丰生物科技有限公司 | A kind of extraction and purification process of dihydromyricetin |
| CN107496415A (en) * | 2017-10-24 | 2017-12-22 | 四川农业大学 | A kind of medicine of the anti-aging based on dihydromyricetin |
| CN109173335A (en) * | 2018-10-18 | 2019-01-11 | 湖北民族学院 | A kind of extraction preparation method visualizing vine tea active constituent |
| CN111303107A (en) * | 2020-03-16 | 2020-06-19 | 重庆骄王天然产物股份有限公司 | Method for extracting dihydromyricetin from vine tea |
| WO2021031622A1 (en) * | 2018-08-23 | 2021-02-25 | 宋昆元 | Method for extracting and separating dihydromyricetin from ampelopsis grossedentata tea |
Citations (1)
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| CN101824018A (en) * | 2010-04-30 | 2010-09-08 | 韩山师范学院 | Method for purifying dihydromyricetin |
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2011
- 2011-04-14 CN CN2011100933467A patent/CN102225923A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101824018A (en) * | 2010-04-30 | 2010-09-08 | 韩山师范学院 | Method for purifying dihydromyricetin |
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Cited By (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2014012075A3 (en) * | 2012-07-13 | 2014-03-06 | Phyto Tech Corp. | Treating hair loss and delaying aging of skin |
| AU2013289963B2 (en) * | 2012-07-13 | 2017-08-24 | Phyto Tech Corp. | Treating hair loss and delaying aging of skin |
| US9789047B2 (en) | 2012-07-13 | 2017-10-17 | Phyto Tech Corp. | Treating hair loss and delaying aging of skin |
| US9949912B2 (en) | 2012-07-13 | 2018-04-24 | Phyto Tech Corp. | Compositions and methods for treating hair loss and delaying aging of skin |
| TWI669116B (en) * | 2012-07-13 | 2019-08-21 | 美商費托科技公司 | Compositions and methods for treating hair loss and delaying aging of skin |
| US10426718B2 (en) | 2012-07-13 | 2019-10-01 | Phyto Tech Corp. | Compositions and methods for treating hair loss and delaying aging of skin |
| CN103772338A (en) * | 2014-01-10 | 2014-05-07 | 吉首大学 | Vacuum pulse type method of preparing dihydromyricetin |
| CN106967030A (en) * | 2017-05-23 | 2017-07-21 | 西安天丰生物科技有限公司 | A kind of extraction and purification process of dihydromyricetin |
| CN107496415A (en) * | 2017-10-24 | 2017-12-22 | 四川农业大学 | A kind of medicine of the anti-aging based on dihydromyricetin |
| WO2021031622A1 (en) * | 2018-08-23 | 2021-02-25 | 宋昆元 | Method for extracting and separating dihydromyricetin from ampelopsis grossedentata tea |
| CN109173335A (en) * | 2018-10-18 | 2019-01-11 | 湖北民族学院 | A kind of extraction preparation method visualizing vine tea active constituent |
| CN111303107A (en) * | 2020-03-16 | 2020-06-19 | 重庆骄王天然产物股份有限公司 | Method for extracting dihydromyricetin from vine tea |
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Application publication date: 20111026 |