CN103554076A - Method for rapidly extracting high-purity dihydromyricetin in ampelopsis grossedentata leaves - Google Patents
Method for rapidly extracting high-purity dihydromyricetin in ampelopsis grossedentata leaves Download PDFInfo
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- CN103554076A CN103554076A CN201310536277.1A CN201310536277A CN103554076A CN 103554076 A CN103554076 A CN 103554076A CN 201310536277 A CN201310536277 A CN 201310536277A CN 103554076 A CN103554076 A CN 103554076A
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07D—HETEROCYCLIC COMPOUNDS
- C07D311/00—Heterocyclic compounds containing six-membered rings having one oxygen atom as the only hetero atom, condensed with other rings
- C07D311/02—Heterocyclic compounds containing six-membered rings having one oxygen atom as the only hetero atom, condensed with other rings ortho- or peri-condensed with carbocyclic rings or ring systems
- C07D311/04—Benzo[b]pyrans, not hydrogenated in the carbocyclic ring
- C07D311/22—Benzo[b]pyrans, not hydrogenated in the carbocyclic ring with oxygen or sulfur atoms directly attached in position 4
- C07D311/26—Benzo[b]pyrans, not hydrogenated in the carbocyclic ring with oxygen or sulfur atoms directly attached in position 4 with aromatic rings attached in position 2 or 3
- C07D311/28—Benzo[b]pyrans, not hydrogenated in the carbocyclic ring with oxygen or sulfur atoms directly attached in position 4 with aromatic rings attached in position 2 or 3 with aromatic rings attached in position 2 only
- C07D311/32—2,3-Dihydro derivatives, e.g. flavanones
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07D—HETEROCYCLIC COMPOUNDS
- C07D311/00—Heterocyclic compounds containing six-membered rings having one oxygen atom as the only hetero atom, condensed with other rings
- C07D311/02—Heterocyclic compounds containing six-membered rings having one oxygen atom as the only hetero atom, condensed with other rings ortho- or peri-condensed with carbocyclic rings or ring systems
- C07D311/04—Benzo[b]pyrans, not hydrogenated in the carbocyclic ring
- C07D311/22—Benzo[b]pyrans, not hydrogenated in the carbocyclic ring with oxygen or sulfur atoms directly attached in position 4
- C07D311/26—Benzo[b]pyrans, not hydrogenated in the carbocyclic ring with oxygen or sulfur atoms directly attached in position 4 with aromatic rings attached in position 2 or 3
- C07D311/40—Separation, e.g. from natural material; Purification
Abstract
The invention discloses a method for rapidly extracting high-purity dihydromyricetin in ampelopsis grossedentata leaves. The method comprises the following steps of (1) smashing dried leaves into 10-20 mesh; (2) immersion cleaning: putting into water and steeping for 6 hours at normal temperature, wherein the massic volume ratio of the material and liquid is 1:100(g/mL); (3) extracting: adding water again, extracting for 60 minutes at 95 DEG C, and separating the heat extracting solution, wherein the massic volume ratio of the material and the liquid is 1:25(g/mL); (4) crystallizing: cooling the extracting solution through refrigeration or at room temperature, promoting crystallization at 4-10 DEG C, filtering, thus obtaining crude product; and (5) recrystallizing: adding boiling water till the crude product is dissolved completely, recrystallizing, filtering mother liquid, thus obtaining the product. In step (1), if the raw material is fresh leaves, the fresh leaves need to be cut into pieces of 1-2mm, in step(2), the massic volume ratio of material and liquid for immersion cleaning is 1:80(g/mL). According to the method, the requirement on rapid preparation of high-purity dihydromyricetin product can be met through steps of immersion cleaning for once, extracting for once, and recrystallizing for once.
Description
Technical field
The invention belongs to Chinese medicinal ingredients extracting method, concrete is a kind of from Ampelopsis grossedentata the method for high efficiency extraction dibydro myricetrin.
Background technology
Ampelopsis grossedentata (
ampelopsisgrossedentata(Hand.-Mazz.) be W. T. Wang) Vitaceae Ampelopsis, be mainly distributed in the area, mountain region, south China of 200～1000 meters of height above sea level, all there is extensive distribution in the provinces such as ,E, Guizhou Province, ,Yue, osmanthus, ,Gan, Hunan, Fujian.Stem is slightly cylindrical, diameter 0.5～3mm, and surperficial yellow-green colour is to yellowish brown, and tool is indulged rib, and matter is crisp, and section shows slightly fibering; Blade is oblong, narrow rhombus, water chestnut shape is avette or lanceolar, long 2～5cm, and wide 1～2cm, there is sawtooth at edge, base portion wedge shape.Among the peoplely drink practice and scientific research result shows, that Ampelopsis grossedentata has is clearing heat and detoxicating, antisepsis and anti-inflammation, dispel rheumatism, the effect such as fat reducing step-down, relieving alcoholism and protecting liver.
Ampelopsis grossedentata leaf is rich in chromocor compound, dibydro myricetrin ((2R wherein, 3R)-3,5,7-trihydroxy--2-(3,4,5-trihydroxy-phenyl) chromanone, Dihydromyricetin) account for general flavone content 80% left and right, already prove core effective component, there is very high exploitation and be worth.Relate to the extraction of dibydro myricetrin, purifying, the Chinese and foreign documents of the aspect such as structural modification and pharmacology reaches 546 pieces, patent of invention has 38, the patent of invention that wherein relates to extractive technique has 14 (a kind of purification process CN201010167579.2 of dibydro myricetrin, a kind of method 200710026288.X that extracts dibydro myricetrin from trifoliate orange seed, the preparation method and its usage 99119123.4 of dibydro myricetrin, extraction of dihydromyricetin from microwave and composition 200410027782.4 thereof, the method 201110458347.7 of while separating dihydromyricetin and ampelopsin from ampelopsis, with porcelain ampelopsis, produce the method CN01114564.1 of dihydromyricetin, a kind of method CN200510032918.5 that extracts dibydro myricetrin from vine tea, a kind of method CN200510102129.4 that extracts dibydro myricetrin from vine tea, dibydro myricetrin new preparation process, pharmaceutical preparation and purposes CN200910066518.4, from vine tea, extract dibydro myricetrin, the method CN201010284838.X of vine tea polysaccharide and vine tea polyphenol, the preparation method CN201110093346.7 of dihydromyricetin from Ampelopsis grossedentata, a kind of method CN201210229991.1 that extracts dibydro myricetrin from vine tea, a kind of method CN201010149014.1 that extracts dibydro myricetrin and vine tea polysaccharide from vine tea, a kind of processing method CN201210401620.7 that extracts high-content dibydro myricetrin from Ampelopsis grossedentata).Document and patent document adopt hydro-thermal extraction, alcohol water extracting method more, coordinate the impurity removal process such as crystallization, column chromatography, purifying after first extracting, step is relatively many, and the soluble impurity of dibydro myricetrin crude extract (polysaccharide, protein, amino acid etc.) is more, need remove by chromatography technique, expend more organic solvent, high expensive.
Carbohydrate, amino acid and other multiple phenoloids are main impurity components.The Polysaccharide A GP-3 being separated in Ampelopsis grossedentata etc. has higher water solubility (Luo Zuyou, Cheng Chao, Li Wei, Wu Moucheng. the separation and purification of Water-soluble Polysaccharides from Ampelopsis grossedentata and the research of character [J]. Food science. 2007,28 (01): 151-154), amino acid and tea polyphenolic compounds have larger solubleness than dibydro myricetrin in water.The solubleness of dibydro myricetrin in cold water is very low, 25 ℃ of solubleness are 0.20mg/mL (Li-Ping Ruan, Bo-Yang Yu, Guang-Miao Fu, Dan-ni Zhu. Improving the solubility of ampelopsin by solid dispersions and inclusion complexes[J]. Journal of Pharmaceutical and Biomedical Analysis. 2005, 38 (3): 457 – 464.), and boiling water solubleness reaches the refined English of 15.90mg/mL(woods. the extraction purifying of dibydro myricetrin and anti-oxidant activity research [D] in Ampelopsis grossedentata. Ph D dissertation, 2004, P51), thereby be convenient to crystallization purifying.The present invention is based on above document and related invention patent, introduce a kind of from Ampelopsis grossedentata leaf the technique of rapid extraction higher degree dibydro myricetrin.
Summary of the invention
The object of the present invention is to provide a kind of method of easy and simple to handle, production cost is low from Ampelopsis grossedentata leaf rapid extraction high purity dibydro myricetrin.
Object of the present invention and solve its technical problem underlying and realize by the following technical solutions: a kind of method of high purity dibydro myricetrin in rapid extraction Ampelopsis grossedentata leaf, includes in operation steps:
(1) pulverize: cured leaf, is crushed to 10～20 orders;
(2) embathe: put into water, feed liquid mass volume ratio is 1:100(g/mL), soak at room temperature 6 hours;
(3) extract: again add water, with 1:25(g/mL) feed liquid mass volume ratio, 95 ℃ are extracted 60 minutes, and separation and Extraction liquid while hot;
(4) crystallization: room temperature or the cooling extracting solution that freezes, promote crystallization, filtration acquisition crude product in 4-10 ℃;
(5) recrystallization: add boiling water to crude product and all dissolve, recrystallization, filter mother liquor, obtains product.
Described operation steps (1), if the bright leaf of starting material, plucking time is no more than 2 days and does not lose large quantity of moisture, must shred to 1-2mm, the feed liquid mass volume ratio that embathes of operation steps (2) is 1:100(g/mL).
The embathing liquid and can reuse 4-5 time of described operation steps (2).
The mother liquor that described operation steps (5) obtains can be recycled and reused for heat of solution crude product, uses continuously five times.
The present invention compared with prior art has obvious advantage and beneficial effect.From above technical scheme,
The invention has the advantages that: adopt and to pulverize-to embathe-extractions-crystallization-recrystallization, first embathe the method for hot water extraction after removal of impurities, rather than removal of impurities after extracting, make the reduction of extract foreign matter content, can carry out crystallization without enrichment step; Technique is simple, easy to operate, and energy consumption is low, has reduced the soluble impurity in dibydro myricetrin hot water extraction liquid, is convenient to be further purified.Wherein:
1, operation steps (2), it is 1:100(g/mL that cured leaf embathes feed liquid mass volume ratio), or bright leaf to embathe feed liquid mass volume ratio be 1:80(g/mL), impurity such as lixiviate carbohydrate that can be relatively large, amino acid, and can not lose a large amount of dibydro myricetrins.
2, operation steps (4) has promotion crystallization, in room temperature is slowly cooling, can improve crystal uniformity coefficient, forms relatively a large amount of nucleus, and treatment capacity greatly needs to freeze cooling, and 4-10 ℃ is conducive to crystal growth, improves yield.
3, the mother liquor that operation steps (5) obtains can be recycled and reused for heat of solution crude product, uses continuously five impacts of the purity on product little.
Embodiment
Below in conjunction with embodiment, method embodiment, feature and effect thereof to high purity dibydro myricetrin in a kind of rapid extraction Ampelopsis grossedentata leaf proposing according to the present invention, be described in detail as follows.
Embodiment 1:
A method for high purity dibydro myricetrin in rapid extraction Ampelopsis grossedentata leaf, it comprises the following steps:
(1) get the dry blade of Ampelopsis grossedentata, be crushed to 10～20 orders;
(2) get the broken leaf 1.0g of preparation, with filter paper, wrap, put into 100mL water, soak at room temperature 6 hours;
(3) take out material bag, drain, add water with 1:25(g/mL) feed liquid mass volume ratio, 95 ℃ are extracted 60 minutes, while hot separation and Extraction liquid;
(4) cooling extracting solution, promotes crystallization, filters and obtains 254mg crude product;
(5) add boiling water to crude product and all dissolve, crystallisation by cooling, filtering mother liquor, obtains 144mg product.
Above-mentioned filter paper is middling speed analytical paper, and above-mentioned dry vanes HPLC detects, and dihydromyricetin cellulose content is 35.27%.
The purity that said process obtains crude product is 69.83%, and the purity of product is 89.01%, and the yield of above-mentioned technique is 36.34%.
Embodiment 2:
A method for high purity dibydro myricetrin in rapid extraction Ampelopsis grossedentata leaf, it comprises the following steps:
(1) get the bright leaf of Ampelopsis grossedentata, cut the broken leaf to 1-2mm;
(2) the broken leaf 100g that gets preparation puts into 8L water, soak at room temperature 6 hours;
(3) liquid is embathed in filtering, adds 2.5L water in 95 ℃ of extractions 60 minutes, while hot separation and Extraction liquid;
(4) cooling extracting solution, promotes crystallization, filters and obtains 9.14g crude product;
(5) add boiling water to crude product and all dissolve, crystallisation by cooling, filtering mother liquor, obtains 4.71g product.
Above-mentioned bright leaf is to be no more than two days the collecting period and not lose too juicy vegetative phase blade or tender leaf; The described liquid that embathes also can be used for embathing of step (2), and described mother liquor also can be used for the recrystallization of step (5).
Above-mentioned bright leaf 5.0g is weighed as 1.212g in 60 ℃ of oven dry, and it is 31.72% that HPLC records cured leaf dihydromyricetin cellulose content, and amounting to dihydromyricetin cellulose content in bright leaf is 13.08%.
The purity that said process obtains crude product is 71.53%, and the purity of product is 94.17%, and the yield of above-mentioned technique is 33.91%.
Embodiment 3:
A method for high purity dibydro myricetrin in rapid extraction Ampelopsis grossedentata leaf, it comprises the following steps:
(1) get the dry blade of Ampelopsis grossedentata, be crushed to 10～20 orders;
(2) get broken leaf 100g and put into 10L water, soak at room temperature 6 hours;
(3) liquid is embathed in filtering, adds 2.5L solvent in 95 ℃ of extractions 60 minutes, while hot separation and Extraction liquid;
(4) cooling extracting solution, promotes crystallization, filters and obtains 26.155g crude product;
(5) add boiling water to crude product and all dissolve, crystallisation by cooling, filtering mother liquor, obtains 13.097g product.
The above-mentioned liquid that embathes also can be used for embathing of step (2), and described mother liquor also can be used for the recrystallization of step (5); Above-mentioned dry vanes HPLC detects, and dihydromyricetin cellulose content is 35.27%.
The purity that said process obtains crude product is 74.51%, and the purity of product is 94.77%; The yield of above-mentioned technique is 35.19%.
Embodiment 4:
A method for high purity dibydro myricetrin in rapid extraction Ampelopsis grossedentata leaf, it comprises the following steps:
(1) get the bright leaf of Ampelopsis grossedentata, cut the broken leaf to 1-2mm;
(2) the broken leaf 1000g that gets preparation puts into 80L water, soak at room temperature 6 hours;
(3) liquid is embathed in filtering, adds 25L water and extracts 60 minutes with 95 ℃, while hot separation and Extraction liquid;
(4) cooling extracting solution, promotes crystallization, filters and obtains 97.58g crude product;
(5) add boiling water to crude product and all dissolve, crystallisation by cooling, filtering mother liquor, obtains 49.90g product.
Above-mentioned bright leaf is to be no more than two days the collecting period and not lose too juicy vegetative phase blade or tender leaf.Described solvent is water, and the described liquid that embathes also can be used for embathing of step (2), and described mother liquor also can be used for the recrystallization of step (5).
Above-mentioned bright leaf 5.0g is weighed as 1.212g in 60 ℃ of oven dry, and it is 31.72% that HPLC records cured leaf dihydromyricetin cellulose content, and amounting to dihydromyricetin cellulose content in bright leaf is 13.08%.
The purity that said process obtains crude product is 68.22%, and the purity of product is 91.84%; The yield of above-mentioned technique is 35.04%.
Embodiment 5:
A method for high purity dibydro myricetrin in rapid extraction Ampelopsis grossedentata leaf, it comprises the following steps:
(1) get the dry blade of Ampelopsis grossedentata, be crushed to 10～20 orders;
(2) the broken leaf 1000g that gets preparation puts into 100L water, soak at room temperature 6 hours;
(3) liquid is embathed in filtering, adds 25L water and extracts 60 minutes with 95 ℃, while hot separation and Extraction liquid;
(4) cooling extracting solution, promotes crystallization, filters and obtains 269.8g crude product;
(5) add boiling water to crude product and all dissolve, crystallisation by cooling, filtering mother liquor, obtains 130.1g product.
The above-mentioned liquid that embathes also can be used for embathing of step (2), and above-mentioned mother liquor also can be used for the recrystallization of step (5).Described dry vanes HPLC detects, and dihydromyricetin cellulose content is 35.27%.
The purity that said process obtains crude product is 70.07%, and the purity of product is 93.08%; The yield of above-mentioned technique is 34.33%.
Embodiment 6:
A method for high purity dibydro myricetrin in rapid extraction Ampelopsis grossedentata leaf, it comprises the following steps:
(1) get the bright leaf of Ampelopsis grossedentata, cut the broken leaf to 1-2mm;
(2) get broken leaf prepared by 10Kg and put into 800L water, soak at room temperature 6 hours;
(3) liquid is embathed in filtering, adds 250L water in 95 ℃ of extractions 60 minutes, while hot separation and Extraction liquid;
(4) cooling extracting solution, promotes crystallization, filters and obtains 901.5g crude product;
(5) add boiling water to crude product and all dissolve, crystallisation by cooling, filtering mother liquor, obtains 475.4g product.
Above-mentioned bright leaf is to be no more than two days the collecting period and not lose too juicy vegetative phase blade or tender leaf; The above-mentioned liquid that embathes also can be used for embathing of step (2), and above-mentioned mother liquor also can be used for the recrystallization of step (5).
Above-mentioned bright leaf 5.0g is weighed as 1.212g in 60 ℃ of oven dry, and it is 31.72% that HPLC records cured leaf dihydromyricetin cellulose content, and amounting to dihydromyricetin cellulose content in bright leaf is 13.08%.
The purity that said process obtains crude product is 77.09%, and the purity of product is 94.18%; The yield of above-mentioned technique is 34.23%.
Embodiment 7:
A method for high purity dibydro myricetrin in rapid extraction Ampelopsis grossedentata leaf, it comprises the following steps:
(1) get the dry blade of Ampelopsis grossedentata, be crushed to 10-20 order;
(2) get broken leaf prepared by 10Kg and put into 1000L water, soak at room temperature 6 hours;
(3) liquid is embathed in filtering, adds 250L water in 95 ℃ of extractions 60 minutes, while hot separation and Extraction liquid;
(4) cooling extracting solution, promotes crystallization, filters and obtains 247.2g crude product;
(5) add boiling water to crude product and all dissolve, crystallisation by cooling, filtering mother liquor, obtains 132.0g product.
The above-mentioned liquid that embathes also can be used for embathing of step (2), and above-mentioned mother liquor also can be used for the recrystallization of step (5).Above-mentioned dry vanes HPLC detects, and dihydromyricetin cellulose content is 35.27%.
The purity that said process obtains crude product is 71.54%, and the purity of product is 92.26%; The yield of above-mentioned technique is 34.53%.
Above embodiment explanation, the present invention has reduced the soluble impurity in extract to greatest extent, make dibydro myricetrin purity in hot water crude extract dry product reach 68.22%-74.51%, far above literature value 40%(, opening friend's victory. isolation identification and the DMY of class tea plant Ampelopsis grossedentata chemical composition obtain through refining technical study [D]. Ph D dissertation, 2001, P67), through recrystallization, obtaining purity is 89.01%-94.77% dibydro myricetrin, and yield is 33.91%-36.34%.The quick preparation requirement that the present invention once embathes, once extraction, a recrystallization can meet higher degree dibydro myricetrin product.
The above, it is only preferred embodiment of the present invention, not the present invention is done to any pro forma restriction, any technical solution of the present invention content that do not depart from, any simple modification, equivalent variations and the modification above embodiment done according to technical spirit of the present invention, all still belong in the scope of technical solution of the present invention.
Claims (4)
1. a method for high purity dibydro myricetrin in rapid extraction Ampelopsis grossedentata leaf, is characterized in that: in operation steps, include:
(1) pulverize: cured leaf, is crushed to 10～20 orders;
(2) embathe: put into water, feed liquid mass volume ratio is 1:100(g/mL), soak at room temperature 6 hours;
(3) extract: again add water, with 1:25(g/mL) feed liquid mass volume ratio, 95 ℃ are extracted 60 minutes, and separation and Extraction liquid while hot;
(4) crystallization: room temperature or the cooling extracting solution that freezes, promote crystallization, filtration acquisition crude product in 4-10 ℃;
(5) recrystallization: add boiling water to crude product and all dissolve, recrystallization, filter mother liquor, obtains product.
2. according to the method for high purity dibydro myricetrin in a kind of rapid extraction Ampelopsis grossedentata leaf claimed in claim 1, it is characterized in that: described operation steps (1), if the bright leaf of starting material, plucking time is no more than 2 days and does not lose large quantity of moisture, must shred to 1-2mm, the feed liquid mass volume ratio that embathes of operation steps (2) is 1:80(g/mL).
3. according to the method for high purity dibydro myricetrin in a kind of rapid extraction Ampelopsis grossedentata leaf claimed in claim 1, it is characterized in that: the embathing liquid and can reuse 4-5 time of operation steps (2).
4. according to the method for high purity dibydro myricetrin in a kind of rapid extraction Ampelopsis grossedentata leaf claimed in claim 1, it is characterized in that: the mother liquor that operation steps (5) obtains can be recycled and reused for heat of solution crude product, uses continuously five times.
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104054962A (en) * | 2014-06-17 | 2014-09-24 | 贵州师范大学 | Application of ampelopsis grossedentata crude extract as pig feed additive |
| CN105153091A (en) * | 2015-08-25 | 2015-12-16 | 华中科技大学同济医学院附属同济医院 | Method for increasing yield of dihydromyricetin in ampelopsis grossedentata |
| CN107892679A (en) * | 2017-11-24 | 2018-04-10 | 贵州大学 | A kind of method of the separating and purifying high-purity dihydromyricetin from ampelopsis |
| CN108047184A (en) * | 2018-01-25 | 2018-05-18 | 张家界久瑞生物科技有限公司 | A kind of method that dihydromyricetin is extracted from ampelopsis grossdentata |
| CN109053666A (en) * | 2018-09-05 | 2018-12-21 | 湖南中茂生物科技有限公司 | A kind of extracting method of dihydromyricetin |
| CN111072618A (en) * | 2019-12-17 | 2020-04-28 | 华南农业大学 | Method for conveniently and rapidly purifying dihydromyricetin from Ampelopsis grossedentata leaves |
| CN111892566A (en) * | 2019-05-05 | 2020-11-06 | 首都医科大学 | Ampelopsis grossedentata water extract, preparation method thereof and application of water extract as acetylcholinesterase inhibitor |
| CN111903812A (en) * | 2020-07-20 | 2020-11-10 | 上海中医药大学 | Instant strawberry tea extract, preparation method and application thereof |
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2013
- 2013-11-04 CN CN201310536277.1A patent/CN103554076A/en active Pending
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Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104054962A (en) * | 2014-06-17 | 2014-09-24 | 贵州师范大学 | Application of ampelopsis grossedentata crude extract as pig feed additive |
| CN105153091A (en) * | 2015-08-25 | 2015-12-16 | 华中科技大学同济医学院附属同济医院 | Method for increasing yield of dihydromyricetin in ampelopsis grossedentata |
| CN105153091B (en) * | 2015-08-25 | 2018-06-12 | 华中科技大学同济医学院附属同济医院 | The method for improving dihydromyricetin yield in ampelopsis grossdentata |
| CN107892679A (en) * | 2017-11-24 | 2018-04-10 | 贵州大学 | A kind of method of the separating and purifying high-purity dihydromyricetin from ampelopsis |
| CN108047184A (en) * | 2018-01-25 | 2018-05-18 | 张家界久瑞生物科技有限公司 | A kind of method that dihydromyricetin is extracted from ampelopsis grossdentata |
| CN109053666A (en) * | 2018-09-05 | 2018-12-21 | 湖南中茂生物科技有限公司 | A kind of extracting method of dihydromyricetin |
| CN111892566A (en) * | 2019-05-05 | 2020-11-06 | 首都医科大学 | Ampelopsis grossedentata water extract, preparation method thereof and application of water extract as acetylcholinesterase inhibitor |
| CN111072618A (en) * | 2019-12-17 | 2020-04-28 | 华南农业大学 | Method for conveniently and rapidly purifying dihydromyricetin from Ampelopsis grossedentata leaves |
| CN111072618B (en) * | 2019-12-17 | 2023-09-05 | 华南农业大学 | Method for conveniently and rapidly purifying dihydromyricetin from self-display ampelopsis grossedentata leaves |
| CN111903812A (en) * | 2020-07-20 | 2020-11-10 | 上海中医药大学 | Instant strawberry tea extract, preparation method and application thereof |
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Application publication date: 20140205 |