CN102988469A - Traditional Chinese medicine composition for preventing and treating influenza virus infection as well as preparation method and application thereof - Google Patents
Traditional Chinese medicine composition for preventing and treating influenza virus infection as well as preparation method and application thereof Download PDFInfo
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Abstract
The invention relates to a traditional Chinese medicine composition for preventing and treating influenza virus infection and a preparation method thereof. The traditional Chinese medicine composition is composed of 10g of roasted tatarian aster root, 10g of purslane and 5.0g of liquorice and can be used for preventing and treating the infection of influenza virus. The traditional Chinese medicine composition can be used for treating flu and can also be used for improving the resistance of patients to the influenza virus. The traditional Chinese medicine composition is preferably suitable for the patients at the beginning of the infection and people might be inflected, can serve as a medicine to be taken at the beginning of the flu and can serve as a health-care product to improve the resistance to the influenza virus. The ethanol extract of the traditional Chinese medicine composition can also be prepared into medical masks and nose drops to prevent the infection of the influenza virus.
Description
Technical field
The present invention relates to a kind of Chinese medicine composition, and the extraction of this Chinese medicine composition, preparation and purposes, the Medicines and Health Product field belonged to.
Background technology
Influenza (abbreviation influenza) is a kind of Acute respiratory infectious disease that is caused by influenza virus, its serious harm human health even life.15～20% people is arranged approximately by influenza infection global every year, worldwide about 500,000 people are dead directly or indirectly to cause every year.From influenza virus in 1918 world wide be very popular cause 2,000 ten thousand people's death since, how to obtain the infection that effective medicine is controlled influenza, be various countries scientist's a important topic.
The process of influenza infection body: influenza virus enters body breeding by steps such as adsorbing → invade → shell → copy → assemble → discharge and infects human body, makes that a large amount of respiratory tract ciliated epithelial cells are infected, degeneration, necrosis and come off; Behind the influenza infection airway epithelial, can form rapidly progeny virus diffusion and infect peripheral cell, cause the vacuolar degeneration of peripheral cell, cause the symptoms such as headache, nasal obstruction, rhinorrhea appear in the infected, unable, the cough of one felting all fagged out, heating.
The Influenza Virus Orthomyxoviridae is a class has special affinity to mucin RNA viruses.The infection of influenza virus mainly relies on hemagglutinin (Hemagglutinin, H) and the neuraminidase (Neuramindase, N) on the peplos.Hemagglutinin is the trimer that is polymerized with non-covalent form by 3 glycosylated polypeptides molecules, and tool is infectious behind protease cracking.The sialic acid receptors bind of HA1 and surface epithelial cell, the HA2 hydrophobic region has the film fusion activity, is necessary to the Virus entry host cell.Neuraminidase has enzymatic activity by the mushroom tetramer that 4 identical glycosylated polypeptides form, and the N-acetyl-neuraminate of hydrolyzable host cell surface glycoprotein end is conducive to ripe Virus release.
The inherent immunity mechanism that participates in the resisiting influenza virus infection mainly contains two kinds, and a kind of is directly to stimulate the host cell that infects to produce IFN-γ behind the influenza infection, and IFN-γ can suppress viral to be copied in host cell; Another kind is the direct killing action of NK cell infected by influenza infection cell; Influenza virus infection can stimulate body to produce corresponding immunne response, thereby various cytokine secretions are increased, and the proinflammatory cytokine that body produces mainly comprises IL-1, IL-2, IL-6, TNF-α, IFN-γ etc.; Granulocyte, IL-6 content is improved, IL-6 is a kind of multifunctional cytokine, and immunity-regulating is replied and activation, growth and the differentiation of the T cell relevant with inflammatory reaction, and can promote the inflammatory reaction that T is cell-mediated.
Chinese medicine is with a long history as traditional medicine, and its significant curative effect that shows in great epidemic disease is at present more and more by concerned.The respiratory tract infection that influenza virus causes belongs to the category of traditional medicine influenza and pathogenic wind-warm, its Chinese traditional treatment comprise direct antivirus action and blocking virus to Normocellular absorption, penetrate, copy etc. and reach antiviral effect, directly antivirus action mainly refers to Chinese medicine directly killing virus, have the single medicinal material great majority of direct antivirus action for the antipyretic and antidotal type medicine, such as Flos Lonicerae, Fructus Forsythiae, Radix Isatidis etc.Chinese medicine also can pass through the apoptosis of the host cell of inhibition influenza infection, and alleviates immunologic injury, keeps the integrity of respiratory mucosa, and performance is to the body protective effect.Therefore, developing some has become inevitable to the medicine that influenza has extensive preventive and therapeutic action.
Designer of the present invention is engaged in the research of medicine resisiting influenza virus for many years.In our experience.The key of influenza infection is the hemagglutinin on the influenza virus peplos, and influenza virus relies on hemagglutinin to be combined with host cell receptor, thereby enters host cell, carries out virus replication, causes pathological changes.The Chinese medicine composition that this test is used is mainly for the hemagglutinin on the property influenza virus peplos, and Chinese medicine composition is with after hemagglutinin contacts, and in conjunction with hemagglutinin, makes hemagglutinin lose ability with the host cell receptor infection, thereby reaches the termination influenza infection.Consult existing document, honey-fried RADIX ASTERIS, Herba Portulacae, Radix Glycyrrhizae are without special toxicity in the Chinese medicine composition, and low dose of avirulence does not produce harm to health.
Summary of the invention
1, the invention provides a kind of Chinese medicine composition of flu-prevention viral infection.This Chinese medicine composition is comprised of honey-fried RADIX ASTERIS 10.0g, Herba Portulacae 1.0g, Radix Glycyrrhizae 5.0g.
2, the present invention further provides the preparation method of this Chinese medicine composition: take by weighing in proportion raw material, soak, decocting boils secondary and filter, filtrate merging concentrates, adds anhydrous edible ethanol, filtration, filtrate distillation, adding distil water are settled to 1g crude drug/ml.
3, further, this preparation method is: claim in proportion honey-fried RADIX ASTERIS 100g, Herba Portulacae 100g, Radix Glycyrrhizae 50.0g.Soak 30min in distilled water, and add 1000ml left and right sides distilled water (liquid level submergence prepared slices of Chinese crude drugs 2-3cm) intense fire and boil, slow fire is fried in shallow oil 30min slowly after its boiling, and medicinal liquid with three layers of filtered through gauze, is got about filtrate 500ml; Continue again to add distilled water to medical material surface in the crude drug and again boil, get about filtrate 500ml, be concentrated into 300-500ml after will twice filtrate merging, and put it into 4 ℃ of refrigerator coolings, treat that it cools off the anhydrous edible ethanol 1000-1200ml of adding in the backward concentrated solution, hold over night fully, next day, filtered through gauze and with filtrate distillation, got the medicinal liquid without edible ethanol, filtrate is settled to 350ml with distilled water, final concentration is 1g crude drug/ml, packing, sterilization, deepfreeze, for subsequent use.
Last three kinds of use of medicinal liquid:
(1) oral, be used for the treatment of influenza virus and the resistance of raising body infected by influenza.
(2) be prepared into the Drug mask: 28 layers of hospital gauze mask are immersed in respectively in the Chinese medicine extraction liquid stock solution, behind the 1h mask taking-up is wrung out medicinal liquid and put it into respectively in the baking oven, 60 ℃-80 ℃ oven dry (24h), cover again one deck without the gauze of medicine on the surface, for subsequent use, (requiring the sterile working) is used for the flu-prevention Viral infection.
(3) be prepared into nasal drop, concentration 1g crude drug/ml, little and packing, for subsequent use after the sterilization, every nostril is dripped 1-2 and is dripped, and is used for the flu-prevention Viral infection.
In the raw material of the present invention, Herba Portulacae is the herb of portulacaceous plant Herba Portulacae; Radix Asteris is the root and rhizome of feverfew Radix Asteris; Radix Glycyrrhizae is the root and rhizome of glycyrrhizic legume, Glycyrrhiza inflata Bat., Glycyrrhiza glabra L..
4, advantage of the present invention:
(1) safety non-toxic of the present invention, inexpensive has practicality.The composition of said composition is clinical Chinese herbal medicine commonly used, and consumption is large, and the toxicity of medicine own is little, and health is not produced harm.
(2) reliable in function of flu-prevention viral infection of the present invention.The present invention is by the inside and outside experimentation of body of this Chinese medicine composition resisiting influenza virus.The result show Chinese medicine composition can make influenza virus liquid (or HA) lose infection activity, suppress its virus replication, enhancing body resistance.
(3) the applicable crowd of the present invention is extensive.In influenza pandemic season, the patient takes this Chinese medicine composition for influenza, can be used as treatment, can be used as health product for population and be used for improving resistance to influenza.Drug mask and nasal drop that Chinese medicine composition is prepared into can be used for the flu-prevention viral infection, reduce the chance of suffering from influenza.
The specific embodiment
1, Chinese medicine composition forms: honey-fried RADIX ASTERIS, Herba Portulacae, Radix Glycyrrhizae.
2, each Chinese medicine ratio of Chinese medicine composition: honey-fried RADIX ASTERIS 10g, Herba Portulacae 10g, Radix Glycyrrhizae 5.0g
3, the extraction of Chinese medicine composition: take by weighing in proportion honey-fried RADIX ASTERIS 100g, Herba Portulacae 100g, Radix Glycyrrhizae 50.0g.Soak 30min in distilled water, and add 1000ml left and right sides distilled water (liquid level submergence prepared slices of Chinese crude drugs 2-3cm) intense fire and boil, slow fire is fried in shallow oil 30min slowly after its boiling, and medicinal liquid with three layers of filtered through gauze, is got about filtrate 500ml; Continue again to add distilled water to medical material surface in the crude drug and again boil, get about filtrate 500ml, be concentrated into 300-500ml after will twice filtrate merging, and put it into 4 ℃ of refrigerator coolings, treat that it cools off the anhydrous edible ethanol 1000-1200ml of adding in the backward concentrated solution, hold over night fully, next day, filtered through gauze and with filtrate distillation, got the medicinal liquid without edible ethanol, filtrate is settled to 350ml with distilled water, final concentration is 1g crude drug/ml, packing, sterilization, deepfreeze, for subsequent use.
Effect embodiment 1
The experiment in vitro research of this Chinese medicine composition resisiting influenza virus hemagglutinin (HA)
The HA that this experiment is used is influenza virus cracking liquid, is produced by Shanghai Vaccine and Serum Institute, and its composition is: contain first 1 type, first 3 types and B-mode influenza virus strain hemagglutinin.After adding Chinese medicine, the hemagglutinative titer of the hemagglutinative titer of medicine group and influenza virus cracking liquid group relatively if hemagglutinative titer reduces, shows that then medicine has the effect of inhibition HA (destroying HA or absorption HA); Do not reduce or change if hemagglutinative titer does not appear in the medicine group, then show medicine infected by influenza HA unrestraint effect.The significant degree of medicine is judged in the change of tiring by red cell agglutination, and the hemagglutinative titer of titration influenza virus cracking liquid just can roughly be estimated the quantity of influenza virus cracking liquid HA integrity.
Operating procedure:
1, medicine two-fold dilution: add normal saline 0.05ml in 96 orifice plate 2-8 holes, add medicine 0.05ml in the 1-2 hole, the 2nd hole mixing is got 0.05ml and is added the 3rd hole, two-fold dilution like this, and then drug level is stock solution, 2
-1, 2
-2, 2
-3, 2
-4, 2
-5, 2
-6, 2
-7
2, the dilution of influenza virus cracking liquid: with the dilution of influenza virus cracking liquid, the agglutination titer of employing virus cracking liquid is 2 external
-8, during experiment, with 2
-7The employing virus cracking liquid of concentration carries out hemagglutination test, and it is 2 that every hole adds concentration
-7Employing virus cracking liquid 0.05ml.
3, add the 0.5%RBC suspension: every hole adds 0.5%RBC suspension 0.05ml, and effect is 2 hours under the room temperature condition, medicine is made effect judge.
Do respectively the erythrocyte matched group, medicine matched group and virus control group under the normal condition of matched group, are made curative effect to medicine and are judged.
4, result
Chinese medicine and influenza virus cracking liquid hemagglutination test result
The specific receptor of HA on the chicken red blood cell film on the+expression influenza virus peplos is combined, thereby red cell agglutination occurs, and namely hemagglutination test is positive.(positive explanation medicine infected by influenza under this concentration is invalid.)
The HA on the-expression influenza virus peplos not specific receptor on chicken red blood cell is combined, and red cell agglutination do not occur, and namely hemagglutination test is negative.(negative explanation medicine infected by influenza under this concentration is inhibited)
The result of the test of hemagglutination test behind the Chinese medicine composition effect different time
Medicine inhibition influenza virus is described, and it doesn't matter with the time that contacts influenza virus HA, also is that the inhibition influenza virus was the same from the effect of 0min-1h after Chinese medicine composition was made the filtration of Drug mask.
5, analysis makes HA lose coagulation function when Chinese medicine composition dilutes (31.25mg/ml) at 1: 64.Illustrate that Chinese medicine composition can suppress the activity of HA under 31.25mg/ml concentration, and with the time-independent that contacts.
Effect embodiment 2
The experiment in vitro research of this Chinese medicine composition resisiting influenza virus
1, the operating procedure hemagglutinative titer is 2
-8Influenza virus liquid by the Drug mask, collect Drug mask filtrate and carry out hemagglutination test, experimental technique is the same, establishes blank normal saline matched group and influenza virus liquid matched group.
2, result
Mask filtrate hemagglutination test result
The specific receptor of HA on the chicken red blood cell film on the+expression influenza virus peplos is combined, thereby red cell agglutination occurs, and namely hemagglutination test is positive.(positive illustrates that filtrate has influenza virus to be combined with RBC and forms the hemagglutination test positive under this concentration)
-expression is combined without the specific receptor of the HA on the influenza virus peplos on chicken red blood cell, red cell agglutination do not occur, and namely hemagglutination test is negative.(negative explanation filtrate influenza virus HA under this concentration has lost activity, and namely medicine has the effect that suppresses influenza virus.)
3, analyze: the hemagglutinative titer of the influenza virus that Medical air spoken parts in traditional operas cover filters is 2
-8The hemagglutinative titer of the influenza virus that the Drug mask filters is from 2
-8Be reduced to 2
-4, can reduce 8 times in conjunction with the quantity of the HA integrity of erythrocyte receptor.Illustrate Chinese medicine composition Drug mask can with the hemagglutinin structure function of influenza virus, suppress preferably the activity of influenza virus.
Effect embodiment 3
The Embryo Gallus domesticus experimentation of this Chinese medicine composition resisiting influenza virus
Influenza virus can be at the chick chorioallantoic membrane cell proliferation, influenza virus after the propagation can be discharged in the allantoic fluid, can make some animal or human's erythrocyte that macroscopic blood clotting phenomenon occurs under certain condition, behind injection of medicine in the influenza virus infection Embryo Gallus domesticus, the blood clotting phenomenon does not appear in allantoic fluid, represents that then medicine has antivirus action.Judge the significant degree of medicine by the change that red cell agglutination is tired, thereby filter out infected by influenza HA inhibiting medicine is arranged, the hemagglutinative titer of titration influenza virus just can roughly be estimated the quantity of influenza virus.
1, medicine is on the toxicity test of Embryo Gallus domesticus: determination experiment is with the impact of medicine on Embryo Gallus domesticus, the result, Embryo Gallus domesticus is all survived after 72 hours, illustrate medicine to Embryo Gallus domesticus without Acute Toxicity.
2, viral EID
50Mensuration: calculate and look into antilogarithm table and get Embryo Gallus domesticus 50 3nfective dose (EID
50) be 10
-5.84/ 0.1ml, namely 10
-5.84Looking into antilogarithm table gets virus liquid and should dilute 140000 times.
3, medicine suppresses the Embryo Gallus domesticus experiment of influenza virus
(1) experiment one
Grouping: select 10 age in days live chickens embryos, be divided at random medicine group, normal saline matched group, normal Embryo Gallus domesticus matched group.Add medicine: except normal Embryo Gallus domesticus matched group is not done any processing, all the other each groups are processed by following method: medicine group: infect viral 20ul (20EID through allantoic cavity first
50), put Embryo Gallus domesticus in 37 ℃ of incubators 60 minutes, then take out, every embryo allantoic cavity is injectable drug 20ul again.Normal saline matched group: in allantoic cavity saline injection 20ul, put embryo in 37 ℃ of incubators.Virus control group: infect virus liquid 20ul (20EID prior to allantoic cavity
50), put embryo and, take out in allantoic cavity saline injection 20ul after 60 minutes in 37 ℃ of incubators.Cultivate: after above egg injection is complete, all with dissolving paraffin wax closed injection hole, puts 37 ℃ of incubators and cultivate.Ovum lamp under one by one observe 2 time picking up every day, and died discards in 24 hours, to 48 hours, the Embryo Gallus domesticus of survival moved to 4 ℃ of refrigerator overnight, gathers in the crops respectively allantoic fluid next day.Hemagglutination test detects: carry out hemagglutination test (HA test) with 0.5% chicken red blood cell.
(2) experiment two
Use 1000EID
50(i.e. 1000 times EID
50Virus dosage) filter the Drug mask, filtrate is inoculated in Embryo Gallus domesticus, measures EID
50, the effect of judgement Drug mask killing influenza virus.
(3) experiment three
Be 2 with hemagglutinative titer
-12Influenza virus liquid by the Drug mask, collect filtrate, consider the liquid inoculated into chick embryo, get allantoic fluid behind the 48h and do hemagglutination test, judge the situation that copies of considering influenza virus in the liquid.
4, result and analysis
The blood coagulation tests result of resisiting influenza virus H1N1 strain in experiment one, the Chinese medicine composition Embryo Gallus domesticus
The specific receptor of HA on the chicken red blood cell film on the+expression influenza virus peplos is combined, thereby red cell agglutination occurs, and namely hemagglutination test is positive.(positive explanation has the specific receptor of influenza virus HA on the chicken red blood cell film to be combined under this allantoic fluid dilution factor, thereby red cell agglutination occurs)
-expression is combined without the specific receptor of the HA on the influenza virus peplos on chicken red blood cell, red cell agglutination do not occur, and namely hemagglutination test is negative.(feminine gender is bright is combined without the specific receptor of influenza virus HA on the chicken red blood cell film under this allantoic fluid dilution factor, thereby red cell agglutination do not occur)
Analyze: the hemagglutinative titer of influenza virus allantoic fluid is 2
-7, honey-fried RADIX ASTERIS, Herba Portulacae, Radix Glycyrrhizae compositions are tired from 2 hirst's hemagglutination
-7Reduce to 2
-4, copying greatly of influenza virus reduced, its quantity has reduced 64 times, illustrates that Chinese medicine composition can suppress copying of influenza virus preferably in Embryo Gallus domesticus.
Experiment two, Drug mask are considered liquid inoculated into chick embryo EID
50Experimental result
Analyze: the EID that is seeded to Embryo Gallus domesticus by the filtrate of Drug mask
50Be 10
-2.25/ 0.1ml is seeded to the EID of Embryo Gallus domesticus by the filtrate of blank mask
50Be 10
-5.84/ 0.1ml, the EID of medicine group
50Reduced 3900 times, illustrated that the influenza virus quantity in the Drug mask filtrate reduces in a large number, the influenza virus quantity of survival is blank mask filtrate 1/3900.Illustrate that the Drug mask can make influenza virus lose activity effectively.
Experiment three, Drug mask are considered the experimental result of liquid inoculated into chick embryo blood clotting
Analyze: be 2 with hemagglutinative titer
-12Influenza virus liquid by blank mask, filtrate is seeded to Embryo Gallus domesticus, hemagglutinative titer is from 2
-12→ 2
13, almost have no change.Be 2 with hemagglutinative titer
-12Influenza virus liquid by the Drug mask, filtrate is seeded to Embryo Gallus domesticus, its hemagglutinative titer is from 2
-12Be reduced to 2
6, the quantity of influenza virus has reduced 64 times.Illustrate that the Drug mask can make influenza virus lose activity effectively.
Effect embodiment 4
This Chinese medicine composition acts on the mdck cell experimentation that H1N1 infects
Mdck cell model after this research adopts influenza virus H1N1 to infect, change by medicine group, normal cell group and influenza virus H1N1 group cell proliferation and drug effect are after influenza virus H1N1 infects mdck cell, the variation of IL-2, IL-6, TNF-α, IFN-γ content in the supernatant, whether enhancing body opposing influenza virus immunity judges to Chinese medicine composition, simultaneously by this supernatant is carried out hemagglutination test, the hemagglutinative titer of titration influenza virus is roughly estimated the quantity of influenza virus.
1, the cultivation of mdck cell: mdck cell is attached cell, adopts 0.25% pancreatin+EDTA had digestive transfer culture.After the mdck cell that covers with monolayer sucked former culture medium, wash one time with D-Hanks liquid, add 3ml 0.25% pancreatin+EDTA, microscopically control digestion time, when treating that cellular contraction change circle comes off nearly, pancreatin inclines, add fresh culture, the cell that blows off gently, low speed centrifuge 1000 turn 5 minutes centrifugal, supernatant and add fresh medium and make packing behind its Eddy diffusion inclines.37 ℃, 5%CO
2Cultivated 24 hours in the incubator.
2, medicine is to the toxic action of cell: with cell by 2 * 10
5Individual/hole joins in 24 orifice plates, cultivates 18h in 37 ℃, 5%CO2 incubator.With the culture fluid that does not contain serum with the different gradients 1/2,1/2 of Chinese medicine two-fold dilution
2, 1/2
3, 1/2
4, 1/2
5, 1/2
6, 1/2
7, and will dilute good Chinese medicine and add respectively 24 orifice plates, 20ul/ hole, last every hole add respectively 10% serum free culture system liquid to the 1mL/ hole, and each group is established multiple hole, cultivates 18h, observation of cell pathological changes situation in 37 ℃, 5%CO2 incubator.The blank group is set up in experiment.The result shows: thus the Chinese medicine extraction liquid that mdck cell is not added into destroys generation cytopathy (CPE), and the concentration of the used Chinese medicine ethanol extract of this illustrative experiment is to the mdck cell avirulence.
3, influenza virus TCID
50Cell is pressed 2 * 10
5Individual/hole joins in 24 orifice plates, cultivates 18h in 37 ℃, 5%CO2 incubator.Discard original fluid, and clean cell 2 times with D-Hanks liquid, use again the culture fluid that does not contain serum with the different gradients 1/2,1/2 of influenza virus H1N1 two-fold dilution
2, 1/2
3, 1/2
4, 1/2
5, 1/2
6, 1/2
7, and the influenza virus H1N1 of dilution added respectively 24 orifice plates, the 20ul/ hole,, last every hole adds respectively 10% serum free culture system liquid to the 1ml/ hole, and each concentration is established 3 multiple holes, cultivates 18h, observation of cell pathological changes situation in 37 ℃, 5%CO2 incubator.The blank group is set up in experiment.
The TCID that this experiment causes with Reed-MuenchShi method Flow Influenza Virus H1N1
50, by above-mentioned, 50%MDCK cytopathy concentration is 10
-4-10
-5Between, calculate the TCID in the mdck cell
50Be 10
-4.333/ 0.1ml, so virus liquid should dilute 27800 times, during experiment, virus liquid concentration is 20TCID
50, namely virus liquid should dilute 1350 times.
4, after the collection mdck cell of supernatant covers with and cultivates the culture bottle wall, add the good influenza virus H of dilution
1N
1(virus liquid concentration is 20TCID
50), siphon away original culture fluid behind the cultivation 2h, add medicinal liquid, (medicine two-fold dilution 1/2,1/2
2, 1/2
3, 1/2
4, 1/2
5, 1/2
6), last every hole adds respectively 2% serum maintenance medium to the 1ml/ hole, collects supernatant after cultivating 24h in 37 ℃, 5%CO2 incubator.
5, the ELISA test kit detects the content of IL-6, IL-2, IFN-γ and TNF-α
Collect supernatant, the by specification operation.
6, hemagglutination test: the cell conditioned medium liquid of collecting is carried out hemagglutination test (HA test), and test method is the same.
7, result and analysis
Chinese medicine acts on the impact of cytokine behind the mdck cell that H1N1 infects
Analyze: honey-fried RADIX ASTERIS, Herba Portulacae, Radix Glycyrrhizae compositions TNF-α and virus control group be (p＜0.05) relatively, and explanation can significantly reduce the TNF-alpha content, suppresses the apoptosis of the host cell of influenza infection, thereby alleviates immunologic injury; Honey-fried RADIX ASTERIS, Herba Portulacae, Radix Glycyrrhizae compositions are compared (P＜0.05) with virus control group IFN-γ content, illustrate that honey-fried RADIX ASTERIS, Herba Portulacae, Radix Glycyrrhizae compositions can promote the secretion of IFN-γ, the exosmose immunne response of blood monocytes and I type helper T lymphocyte (Th1) of acceleration, thereby various cytokine secretions are increased, the enhancing human body immunity function.
Chinese medicine acts on the mdck cell supernatant hemagglutination test that H1N1 infects
The specific receptor of HA on the chicken red blood cell film on the+expression influenza virus peplos is combined, thereby red cell agglutination occurs, and namely hemagglutination test is positive.(positive explanation medicine has hemagglutinin to be combined with RBC under this dilution factor)
The HA on the-expression influenza virus peplos not specific receptor on chicken red blood cell is combined, and red cell agglutination do not occur, and namely hemagglutination test is negative.(positive explanation medicine is combined with RBC without hemagglutinin under this dilution factor)
Analyze: to tire be 2 to hirst's hemagglutination in the cell conditioned medium liquid hemagglutination test
-8, honey-fried RADIX ASTERIS, Herba Portulacae, Radix Glycyrrhizae compositions group hemagglutination test Chinese medicine diluted concentration (1: 2
3) when being 0.125g/ml, influenza virus is lost activity, can judge that medicine can suppress influenza virus and copy when 0.125g/ml.Illustrate that honey-fried RADIX ASTERIS, Herba Portulacae, Radix Glycyrrhizae compositions can make the influenza virus inactivation effectively in cell model.
Claims (7)
1. a Chinese medicine composition that is used for controlling in advance influenza infection is characterized in that described Chinese medicine composition is by being comprised of honey-fried RADIX ASTERIS, Herba Portulacae, Radix Glycyrrhizae, and its ratio is honey-fried RADIX ASTERIS 10g, Herba Portulacae 10g, Radix Glycyrrhizae 5.0g.
2. the preparation method of Chinese medicine composition for the control influenza infection according to claim 1, it is characterized in that: take by weighing in proportion raw material, soak, decocting boils secondary and filter, filtrate merging concentrates, adds anhydrous edible ethanol, filtration, filtrate distillation, adding distil water are settled to 1g crude drug/ml.
3. the preparation method of Chinese medicine composition for the control influenza infection according to claim 2 is characterized in that, prepares in accordance with the following steps and gets: claim in proportion honey-fried RADIX ASTERIS 100g, Herba Portulacae 100g, Radix Glycyrrhizae 50g.Soak 30min in distilled water, and add 1000ml left and right sides distilled water (liquid level submergence prepared slices of Chinese crude drugs 2-3cm) intense fire and boil, slow fire is fried in shallow oil 30min slowly after its boiling, and medicinal liquid with three layers of filtered through gauze, is got about filtrate 500ml; Continue again to add distilled water to medical material surface in the crude drug and again boil, get about filtrate 500ml, be concentrated into 300-500ml after will twice filtrate merging, and put it into 4 ℃ of refrigerator coolings, treat that it cools off the anhydrous edible ethanol 1000-1200ml of adding in the backward concentrated solution, hold over night fully, next day, filtered through gauze and with filtrate distillation, got the medicinal liquid without edible ethanol, filtrate is settled to 350ml with distilled water, final concentration is 1g crude drug/ml, packing, sterilization, deepfreeze, for subsequent use.
4. Chinese medicine composition according to claim 1 is in the medicine of preparation control influenza virus or the purposes in the medical supplies.
5. medicine according to claim 4 is characterized in that said composition is prepared into decoction oral, is used for the treatment of influenza infection.
6. medicine according to claim 4 is characterized in that said composition is prepared into nasal drop, and concentration is 1g crude drug/ml, little and packing, and for subsequent use after the sterilization, every nostril is dripped 1-2 and is dripped, and is used for the flu-prevention Viral infection.
7. medical supplies according to claim 4 is characterized in that said composition is prepared into the external of Drug mask, are used for the flu-prevention Viral infection.
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| CN104116802A (en) * | 2014-07-10 | 2014-10-29 | 扬中牧乐药业有限公司 | Aster and fructus forsythiae respiratory disease dysentery rehabilitation mixture for chicken |
| CN105687674A (en) * | 2016-03-29 | 2016-06-22 | 张渝 | Traditional Chinese medicine composition and preparation method thereof |
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| CN104116802A (en) * | 2014-07-10 | 2014-10-29 | 扬中牧乐药业有限公司 | Aster and fructus forsythiae respiratory disease dysentery rehabilitation mixture for chicken |
| CN105687674A (en) * | 2016-03-29 | 2016-06-22 | 张渝 | Traditional Chinese medicine composition and preparation method thereof |
| CN108433214A (en) * | 2018-02-08 | 2018-08-24 | 海宁爱康医疗科技有限公司 | One kind being used for medicated gauze mask welding packing apparatus application method |
| CN108936891A (en) * | 2018-09-20 | 2018-12-07 | 合肥巧织纺织科技有限公司 | A kind of anti-mite antibacterial nonwoven cloth medical mask |
| CN111214624A (en) * | 2020-04-08 | 2020-06-02 | 国华鹏源股份有限公司 | Novel virus cold tablet |
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